Indole-3 Butyric Acid
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Auxins Cytokinins and Gibberellins TD-I Date: 3/4/2019 Cell Enlargement in Young Leaves, Tissue Differentiation, Flowering, Fruiting, and Delay of Aging in Leaves
Informational TD-I Revision 2.0 Creation Date: 7/3/2014 Revision Date: 3/4/2019 Auxins, Cytokinins and Gibberellins Isolation of the first Cytokinin Growing cells in a tissue culture medium composed in part of coconut milk led to the realization that some substance in coconut milk promotes cell division. The “milk’ of the coconut is actually a liquid endosperm containing large numbers of nuclei. It was from kernels of corn, however, that the substance was first isolated in 1964, twenty years after its presence in coconut milk was known. The substance obtained from corn is called zeatin, and it is one of many cytokinins. What is a Growth Regulator? Plant Cell Growth regulators (e.g. Auxins, Cytokinins and Gibberellins) - Plant hormones play an important role in growth and differentiation of cultured cells and tissues. There are many classes of plant growth regulators used in culture media involves namely: Auxins, Cytokinins, Gibberellins, Abscisic acid, Ethylene, 6 BAP (6 Benzyladenine), IAA (Indole Acetic Acid), IBA (Indole-3-Butyric Acid), Zeatin and trans Zeatin Riboside. The Auxins facilitate cell division and root differentiation. Auxins induce cell division, cell elongation, and formation of callus in cultures. For example, 2,4-dichlorophenoxy acetic acid is one of the most commonly added auxins in plant cell cultures. The Cytokinins induce cell division and differentiation. Cytokinins promote RNA synthesis and stimulate protein and enzyme activities in tissues. Kinetin and benzyl-aminopurine are the most frequently used cytokinins in plant cell cultures. The Gibberellins is mainly used to induce plantlet formation from adventive embryos formed in culture. -
Addition of Abscisic Acid Increases the Production of Chitin Deacetylase By
Process Biochemistry 51 (2016) 959–966 Contents lists available at ScienceDirect Process Biochemistry jo urnal homepage: www.elsevier.com/locate/procbio Addition of abscisic acid increases the production of chitin deacetylase by Colletotrichum gloeosporioides in submerged culture a a b b Angelica Ramos-Puebla , Carolina De Santiago , Stéphane Trombotto , Laurent David , c a,∗ Claudia Patricia Larralde-Corona , Keiko Shirai a Universidad Autonoma Metropolitana-Iztapalapa, Biotechnology Department, Laboratory of Biopolymers and Pilot Plant of Bioprocessing of Agro-Industrial and Food By-Products, Av. San Rafael Atlixco, No. 186, 09340 Mexico City, Mexico b Ingénierie des Matériaux Polymères IMP@Lyon1 UMR CNRS 5223, Université Claude Bernard Lyon 1, Université de Lyon, 15 bd A. Latarjet, Villeurbanne Cedex, France c Centro de Biotecnología Genómica Instituto Politécnico Nacional, Tamaulipas, Mexico a r t i c l e i n f o a b s t r a c t Article history: The activity of chitin deacetylase from Colletotrichum gloeosporioides was studied by the addition of phy- Received 26 December 2015 tohormones (gibberellic acid, indole acetic acid, and abscisic acid) and amino sugars (glucosamine and Received in revised form 1 May 2016 N-acetyl glucosamine) in culture media. Abscisic acid exerted a positive and significant effect on enzyme Accepted 2 May 2016 −1 −1 production with 9.5-fold higher activity (1.05 U mg protein ) than the control (0.11 U mg protein ). Available online 3 May 2016 Subsequently, this phytohormone was used in batch culture with higher chitin deacetylase activity being found at acidic pH (3.5) than at neutral pH (7). Furthermore, the highest activity was determined at the Chemical compounds studied in this article: acceleration growth phase. -
The Effects of Cytokinin Types and Their Concentration on in Vitro Multiplication of Sweet Cherry Cv
The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.) Dj. V. Ružić, T. I. Vujović Fruit Research Institute, Čačak, Republic of Serbia ABSTRACT: Determination of the most optimal types and concentrations of plant growth regulators as medium constituents is one of the most important aspects of successful micropropagation, among other in vitro factors. With the aim of optimization of in vitro multiplication of sweet cherry cv. Lapins the effect of following cytokinins has been studied: benzyladenine (BA), isopentenyl adenine (2iP), kinetin (KIN) and thidiazuron (TDZ) at concentrations of 1, 2, 5, 10 and 15μM, combined with auxine, indole-3-butyric acid (IBA) at concentrations of 0, 0.5, 2.5 and 5μM. Murashige and Skoog (1962) was the basic medium used in all the combinations. The following multiplication pa- rameters were monitored: multiplication index, length of axial and lateral shoots. Fresh and dry shoot weight (callus, stem and leaves – S + L) were determined. Some specific issues, such as colour, leaf and callus size, leaf roll, incidence of chlorosis or necrosis along with occurrence of rhizogenesis, i.e. roots unusual for this phase of micropropagation, were also monitored. The highest multiplication index as well as length of axial and lateral shoots was obtained on media with BA. Very poor multiplication, with large sized shoots and big leaves, was achieved on media with 2iP, TDZ and KIN, whereas in many combinations with 2iP, and particularly in those with KIN, rhizogenesis was induced. Obtained results suggest that the choice of cytokinins for the phase of multiplication of sweet cherry is limited to BA. -
The Effect of Crosstalk Between Abscisic Acid (ABA) And
Genetic Manipulation of the Cross-talk between Abscisic Acid and Strigolactones and Their Biosynthetic Link during Late Tillering in Barley Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften (Dr. rer. nat.) Der Naturwissenschaftlichen Fakultät I Biowissenschaften der Martin-Luther-Universität Halle-Wittenberg, vorgelegt von Herrn M.Sc. Hongwen Wang geb. am 10.02.1985 in Gaomi, China Gutachter: 1. Prof. Dr. Nicolaus von Wirén 2. Prof. Dr. Klaus Humbeck 3. Prof. Dr. Harro J. Bouwmeester Datum der Verteidigung: 27.04.2017, Halle (Saale) Contents 1. Introduction ............................................................................................................................ 1 1.1 Genetics of tillering in barley ........................................................................................... 1 1.2 Functional role of abscisic acid in branch or tiller development ...................................... 4 1.3 Abscisic acid biosynthesis and metabolism ...................................................................... 5 1.4 Functional role of strigolactones in branch or tiller development .................................... 7 1.5 Biosynthetic pathway of strigolactones ............................................................................ 8 1.6 The cross-talk between abscisic acid and strigolactones biosynthetic pathways ........... 10 1.7 Aim of the present study ..................................................................................................11 2. Materials and methods ........................................................................................................ -
5 1China Agricultural University, Beijing, China; 2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
Abscisic acid Jigang Li1, Yaorong Wu2, Qi Xie2, Zhizhong Gong1 5 1China Agricultural University, Beijing, China; 2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China Summary The classical plant hormone abscisic acid (ABA) was discovered over 50 years ago. ABA accumulates rapidly in plants in response to environmental stresses, such as drought, cold, or high salinity, and plays important roles in the adaptation to and survival of these stresses. This “stress hormone” also functions in many other processes throughout the plant life cycle, acting in embryo development and seed maturation, seed dormancy and germination, seed- ling establishment, vegetative development, root growth, stomatal movement, flowering, pathogen response, and senescence. It is transported in the vascular tissues to coordinate root and shoot development and function. Receptors for ABA have been identified as a family of soluble proteins, which upon binding ABA form coreceptor complexes with phosphoprotein phosphatase 2C (PP2C) phosphoprotein phosphatases. The resulting inhibition of activity of PP2C enzymes leads to changes in phosphorylation of protein kinases and transcription fac- tors, to mediate the multiple effects of ABA. The elucidation of ABA perception mechanisms and the core components of the signal transduction mechanisms from ABA perception to downstream gene expression has expanded our understanding of the functions of ABA. This chapter summarizes our current understanding of the key components of ABA metabolism, transport, physiological functions, signal transduction, gene expression, and proteolysis. 5.1 Discovery and functions of abscisic acid Abscisic acid (ABA), a classic plant hormone, was isolated multiple times in differ- ent studies. Researchers in the early 1950s isolated acidic compounds, referred to as β-inhibitors, from plants; they separated these compounds by paper chromatography and showed that β-inhibitors inhibit coleoptile elongation in oat. -
On the Biosynthesis and Evolution of Apocarotenoid Plant Growth Regulators
On the biosynthesis and evolution of apocarotenoid plant growth regulators. Item Type Article Authors Wang, Jian You; Lin, Pei-Yu; Al-Babili, Salim Citation Wang, J. Y., Lin, P.-Y., & Al-Babili, S. (2020). On the biosynthesis and evolution of apocarotenoid plant growth regulators. Seminars in Cell & Developmental Biology. doi:10.1016/ j.semcdb.2020.07.007 Eprint version Post-print DOI 10.1016/j.semcdb.2020.07.007 Publisher Elsevier BV Journal Seminars in cell & developmental biology Rights NOTICE: this is the author’s version of a work that was accepted for publication in Seminars in cell & developmental biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Seminars in cell & developmental biology, [, , (2020-08-01)] DOI: 10.1016/j.semcdb.2020.07.007 . © 2020. This manuscript version is made available under the CC- BY-NC-ND 4.0 license http://creativecommons.org/licenses/by- nc-nd/4.0/ Download date 27/09/2021 08:08:14 Link to Item http://hdl.handle.net/10754/664532 1 On the Biosynthesis and Evolution of Apocarotenoid Plant Growth Regulators 2 Jian You Wanga,1, Pei-Yu Lina,1 and Salim Al-Babilia,* 3 Affiliations: 4 a The BioActives Lab, Center for Desert Agriculture (CDA), Biological and Environment Science 5 and Engineering (BESE), King Abdullah University of Science and Technology, Thuwal, Saudi 6 Arabia. -
ABSCISIC ACID Dr. Uttam Kumar Kanp
ABSCISIC ACID Dr. Uttam Kumar Kanp Contents: 1. Discovery of ABA 2. Chemical Nature 3. Bioassay of ABA 4. Physiological roles of Abscisic Acid 5. Biosynthesis of AB A in Plants 6. Degradation/Inactivation of AB A in Plants 7. Occurrence and Distribu tion of ABA in Plants and 8. ABA Transport in Plant. 1. Discovery of ABA: In 1963, a substance strongly antagonistic to growth was isolated by F. T. Add icott from young cotton fruits and named Abscisin II. Later on, this name was changed to Abscisic acid (ABA). The chemical name of abscisic acid whose structure is given in fig. 1. Is [3-methyl 5-1′ (1′- hydrox y, 4′-oxy-2′, 6′, 6′-trimethyl-2- cyclohexane-l-yl)-cis, trans-2,4- penta-dienoic acid]. Eagles and Wareing (1963, 64), at the same time pointed out the presence of a substance in birch leaves (Betula pubescens, a decidu ous plant) which inhibited growth and induced dormancy of buds and, therefore, named it ‘dormin’. But, very soon as a result of the work of Cornfort h et. al. (1965), it BOTANY: SEM – V, PAPE R-C12T: PLANT PHYSIOLOGY, UNIT -5: PLANT GROWTH REGULATORS – ABSCISIC ACID was found to be identical with absc isic acid. 2. Chemical Nature of ABA: Abscisic acid is a 15-C sesquiterpene compound (molecular formula C15H20O4) composed of three isoprene residues and having a cyclohexane ring with keto and one hydroxyl group and a side chain with a terminal car boxylic group in its structure. ABA resembles terminal portion of some carotenoids such as violaxanthin and neoxanthin (see Fig. -
Using Plant Growth Regulators (PGR) in Citrus
Using Plant Growth Regulators (PGR) in Citrus Ashraf El-Kereamy UCCE Specialist & UCLREC director Department of Botany and Plant Sciences UC Riverside [email protected] 08/19/2020 Presentation outline • Overview of Plant Growth Regulators (PGR) • Synthesis and function of different plant hormones • Plant hormones vs PGR • Categories and the mode of action of PGR • Handling of registered PGR in citrus • PGRs role in preventing fruit disorder • Using PGR to improve fruit set and fruit size • Reducing fruit drop by PGRs • PGR and alternate bearing in citrus • PGR to control suckering and tree size • Discussion and participants perspectives PGR vs Hormones Plant Growth Regulators (PGR): - Synthetic form of the plant hormones which can be used to control or modify plant growth, also called plant growth substances or growth factors Plant hormones: - Endogenous organic compounds active at very low concentration - Essential for regulating plant growth and development - Produced in one tissue and translocated to another tissue - Have a specific function at specific stages and concentrations - They act together in a complex pathway Classical plant hormones synthesis and function Hormone Where produced or found Function Auxin -Embryos -Stimulates stem elongation at low concentration -Meristems of apical buds and young leaves -Delays color and ripening -Retards abscission Cytokinin -Roots -Affects root growth -Stimulates cell division and branching -Delays ripening and senescence -Increases fruit set Gibberellins -Embryos -Promote bud growth and seed -
33Rd Meeting of the Association Was Held at Whistler, British Columbia
PROCEEDINGS OF THE THIRTY-THIRD MEETING OF THE CANADIAN FOREST GENETICS ASSOCIATION PART 1 Minutes and Members’ Reports PART 2 Symposium COMPTES RENDUS DU TRENTE-TROISIÈME CONGRÈS DE L’ASSOCIATION CANADIENNE DE GÉNÉTIQUE FORESTIÈRE 1ere PARTIE Procès-verbaux et rapports des membres 2e PARTIE Colloque National Library of Canada cataloguing in publication data Canadian Forest Genetics Association. Meeting (33rd : 2013 : Whistler, BC) Proceedings of the Thirty-third Meeting of the Canadian Forest Genetics Association Includes preliminary text and articles in French. Contents : Part 1. Minutes and Member's Reports. Part 2. Symposium. Fo1-16/2017-PDF 978-0-660-06674-5 1. Forest genetics – Congresses. 2. Trees – Breeding – Congresses. 3. Forest genetics – Canada – Congresses. I. Atlantic Forestry Centre. II. Title. III. Title : Proceedings of the Thirty-third Meeting of the Canadian Forest Genetics Association Données de catalogage avant publication de la Bibliothèque nationale du Canada l'Association canadienne de génétique forestière. Conférence (33e : 2013 : Whistler, C-B) Comptes rendus du trente-troisième congrès de l'Association canadienne de génétique forestière Comprend des textes préliminaires et des articles en français. Sommaire : 1ère Partie. Procès-verbaux et rapports des membres. 2e Partie. Colloque. Fo1-16/2017-PDF 978-0-660-06674-5 1. Génétiques forestières – Congrès. 2. Arbres – Amélioration – Congrès. 3. Génétiques forestières – Canada – Congrès. I. Centre de foresterie de l'Atlantique. II. Titre. III. Titre : Comptes rendus du trente-troisième congrès de l'Association canadienne de génétique forestière PROCEEDINGS OF THE THIRTY-THIRD MEETING OF THE CANADIAN FOREST GENETICS ASSOCIATION PART 1 Minutes and members’ reports Whistler, British Columbia July 22–25, 2013 Editor J.D. -
Plant Growth Regulator ANALYSIS (0.150% KINETIN, 0.075% IBA, 0.050% GIBBERELLIC ACID)
Plant Growth Regulator ANALYSIS (0.150% KINETIN, 0.075% IBA, 0.050% GIBBERELLIC ACID) WHAT IS IT? • Elite is a CFIA registered plant growth regulator (Fert. Act # 2016005A) • It contains auxins (Indole-3-Butyric Acid, IBA), cytokinins (6-Furfurylaminopurine, Kinetin) and gibberellins (Gibberellic acid, GA). • The product is available in 2 x 6L cases. WHEN & WHY USE IT? • Elite is recommended on a variety of field crops, vegetables and fruit trees. • Elite is for use with in-furrow applied starter fertilizer, through drip irrigation or as a foliar. • The product contains auxin, which is known to activate cell elongation by increasing the level of elasticity of the cell walls. • Auxins stimulate ethylene production and inhibit the growth of buds. • They also promote adventitious and lateral root growth and development. • Elite also contains kinetin, which is the only Canadian registered cytokinin that can be used on a variety of crop species. • Kinetin increases the rate of cell division, differentiation and growth. • It delays senescence in plant tissues, increases flower set, fruit formation and side branching. • The GA contained in Elite enables seed to overcome dormancy and promote the activity of the alpha-amylase, sprouting and emergence. Application Guidelines • GA affects cell elongation and can increase seed and fruit set when applied appropriately. • Rate varies from 62ml/ac to 162 ml/ac • The combination of these three families of plant growth regulators, at the right depending on application method dosage, under stress condition, can lead to a substantial enhancement of growth and stage of the crop. and development, seed and fruit set and ultimately yield. -
The Properties and Interaction of Auxins and Cytokinins Influence Rooting of Shoot Cultures of Eucalyptus
African Journal of Biotechnology Vol. 11(100), pp. 16568-16578, 13 December, 2012 Available online at http://www.academicjournals.org/AJB DOI: 10.5897/AJB12.1523 ISSN 1684–5315 ©2012 Academic Journals Full Length Research Paper The properties and interaction of auxins and cytokinins influence rooting of shoot cultures of Eucalyptus Muhammad Nakhooda1*, M. Paula Watt1 and David Mycock2 1School of Life Sciences, University of KwaZulu-Natal, Durban, South Africa. 2School of Animal, Plant and Environmental Sciences, University of the Witwatersrand, Johannesburg, South Africa. Accepted 16 October, 2012 Success in Eucalyptus micropropagation varies with genotype. Although some protocols have proven suitable for suites of clones, many genotypes are recalcitrant to rooting. Their micropropagation is addressed empirically through the manipulation of auxins and cytokinins, which work antagonistically to produce roots and shoots, respectively. Rooting success of three genotypes with 0.1 mg/l indole-3- butyric acid (IBA) was initially recorded as 87, 45 and 41% for clones 1, 2 and 3, respectively. Further studies using the auxin signal transduction inhibitor ρ-chlorophenoxyisobutyric acid (PCIB) or the auxin conjugation inhibitor dihydroxyacetophenone (DHAP) indicated that the poor rooting response of clone 2 was not due to deficient auxin signal perception or auxin conjugation. Omitting kinetin during elongation, followed by auxin-free rooting, significantly increased root production in clone 2 (from 45 to 80.3%), but had no effect on clone 1. Gas chromatography-mass spectrometry (GC-MS) analysis of auxins and kinetin of shoots, prior to rooting, revealed a strong relationship (R2 = 0.943) between rootability and the shoot kinetin:auxin. Replacing kinetin with the less stable trans-zeatin significantly increased rooting of clone 2 (from 19 to 45%) and clone 3 (31 to 52%). -
Give Your Turf a Boost
for Turf & Ornamentals Give Your Turf A Boost Agra-Rouse™ is a blend of bio-stimulants containing naturally-occurring and synthetic plant growth regulators and plant hormones that give you a boost of healthy, sustained growth on your turf, trees and ornamentals. How it works: Agra-Rouse is a mix of plant hormones and bio-stimulants known to enhance plant yields, promote cell division and encourage root development and propagation. It works with the plant’s own natural physiology to stimulate growth, vigor and health. Agra-Rouse may be used as a supplement to fertilizers either as part of the Performance Foliar Treatment™ What it does: program or applied as part of the Performance Soil Treatment™. • Stimulates plant growth and development Where to use it: • Enhances cell division Lawns • Trees • Golf Courses • Parks • Athletic Fields • Greenhouse & Field Ornamentals Turf Seed Production • Sod Production • Increases cell differentiation • Promotes cell enlargement Turf • Develops root growth Agra-Rouse has the ability to help turf recover after periods of heavy traffic or high stress. On newly applied sod, Agra-Rouse improves establishment by encouraging new root growth and • Aids nutrient use efficiency root penetration of the soil. It also aids to improve resistance to winter kill and frost damage. Agra-Rouse helps to break the dormancy of Bermudagrass, Zoysiagrass and Paspalums. In high traffic areas, in weak areas otherwise slow to recover, and on tee complexes, Agra- Benefits you may expect: Rouse promotes growth. When sprayed prior to aerification, Agra-Rouse helps core holes • Faster seed germination to close faster. It aids in the recovery from pesiticide damange and can even heal from the bronzing effect caused by some PGR’s.