Datasheet PB0639 Anti-KIN Antibody

Product datasheet Anti-KIN Antibody Catalog Number: PB0639

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-KIN Antibody Gene Name KIN Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,IHC-P Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human KIN recombinant protein (Position: K3-K202). Human KIN shares 98% amino acid (aa) sequence identity with mouse KIN. Purification Immunogen affinity purified. Observed MW 45KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry(Paraffin-embedded Section): 1:50-400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information DNA/RNA-binding protein KIN17, also known as BTCD or KIN17, is a protein that in humans is encoded by the KIN gene. This gene is mapped to 10p14. The protein encoded by this gene is a nuclear protein that forms intranuclear foci during proliferation and is redistributed in the nucleoplasm during the cell cycle. Short-wave ultraviolet light provokes the relocalization of the protein, suggesting its participation in the cellular response to DNA damage. Originally selected based on protein-binding with RecA antibodies, the mouse protein presents a limited similarity with a functional domain of the bacterial RecA protein, a characteristic shared by this human ortholog. Alternative splicing of this gene results in multiple transcript variants.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-KIN Antibody Catalog Number: PB0639

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. IHC analysis of analysis of KIN using anti- KIN using anti-KIN KIN antibody (PB0639). antibody (PB0639).KIN Electrophoresis was was detected in paraffin- performed on a 5-20% embedded section of SDS-PAGE gel at 70V Human Oesophagus (Stacking gel) / 90V Squama Cancer Tissue. (Resolving gel) for 2-3 Heat mediated antigen hours. The sample well of retrieval was performed each lane was loaded in citrate buffer (pH6, with 50ug of sample epitope retrieval solution) under reducing for 20 mins. The tissue conditions. Lane 1: HELA section was blocked with Whole Cell Lysate,Lane 2: 10% goat serum. The 22RV1 Whole Cell tissue section was then Lysate,Lane 3: COLO320 incubated with 1μg/ml Whole Cell Lysate,Lane 4: rabbit anti-KIN Antibody MCF-7 Whole Cell (PB0639) overnight at Lysate,Lane 5: U20S 4°C. Biotinylated goat Whole Cell Lysate,Lane 6: anti-rabbit IgG was used NIH3T3 Whole Cell as secondary antibody Lysate. After and incubated for 30 Electrophoresis, proteins minutes at 37°C. The were transferred to a tissue section was Nitrocellulose membrane developed using Strepavi at 150mA for 50-90 din-Biotin-Complex minutes. Blocked the (SABC)(Catalog # membrane with 5% Non- SA1022) with DAB as the fat Milk/ TBS for 1.5 hour chromogen. at RT. The membrane was incubated with rabbit anti- KIN antigen affinity purified polyclonal antibody (Catalog # PB0639) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-KIN Antibody Catalog Number: PB0639

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

specific band was detected for KIN at approximately 45KD. The expected band size for KIN is at 45KD.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3