ANTICANCER RESEARCH 31: (2011)

Instructions to Authors

General Policy. ANTICANCER RESEARCH (AR) will accept original high quality works and reviews on all aspects of experimental and clinical cancer research. The Editorial Policy suggests that priority will be given to papers advancing the understanding of cancer causation, and to papers applying the results of basic research to cancer diagnosis, prognosis, and therapy. AR will also accept the following for publication: (a) Abstracts of scientific meetings on cancer, following consideration and approval by the Editorial Board; (b) Announcements of meetings related to cancer research; (c) Short reviews (of approximately 120 words) and announcements of newly received books and journals related to cancer, and (d) Announcements of awards and prizes. The principal aim of AR is to provide for the prompt publication of original works of high quality, generally within 1-2 months from final acceptance. Manuscripts will be accepted on the understanding that they report original unpublished works on the cancer problem that are not under consideration for publication by another journal, and that they will not be published again in the same form. All material submitted to AR will be subject to review, when appropriate, by two members of the Editorial Board and by one suitable outside referee. The Editors reserve the right to improve manuscripts on grammar and style. The Editors and Publishers of AR accept no responsibility for the contents and opinions expressed by the contributors. Authors should warrantee due diligence in the creation and issuance of their work. NIH Open Access Policy. The journal acknowledges that authors of NIH funded research retain the right to provide a copy of the final manuscript to the NIH four months after publication in ANTICANCER RESEARCH, for public archiving in PubMed Central. Copyright. 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Examples: 1 Sumner AT: The nature of chromosome bands and their significance for cancer research. Anticancer Res 1: 205-216, 1981. 2 McGuire WL and Chamnes GC: Studies on the oestrogen receptor in breast cancer. In: Receptors for Reproductive Hormones (O' Malley BW, Chamnes GC (eds.). New York, Plenum Publ Corp., pp 113-136, 1973. ANTICANCER RESEARCH 31: (2011)

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Copyright © 2011 - International Institute of Anticancer Research (J.G. Delinassios). All rights reserved (including those of translation into other languages). No part of this journal may be reproduced, stored in a retrieval system, or transmitter in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher. 22th ANNUAL MEETING OF THE ITALIAN SOCIETY OF URO-ONCOLOGY (SIUrO) Bologna, June 13-15, 2012 Savoia Hotels, Via del Pilastro, 2 Bologna, Italy

Deadline for abstract submission: February 3, 2012

Chair Giuseppe Martorana

Advisory Committee Alessandro Bertaccini Eugenio Brunocilla Marco Garofalo Fabio Manferrari Riccardo Schiavina

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Italian Society of Uro-Oncology (SIUrO) President: Giuseppe Martorana, Bologna, Italy CANCER

GENOMICS & www.iiar-anticancer.org, www.iiarjournals.org Print ISSN: 1109-6535 Online ISSN: 1790-6245 ROTEOMICS VOL. 1 (2004), VOL. 2 (2005), VOL. 3 (2006), VOL. 4 (2007), P VOL. 5 (2008), VOL. 6 (2009), VOL. 7 (2010), VOL. 8 (2011) General Policy. CANCER GENOMICS & PROTEOMICS (CGP) welcomes submissions of original high quality articles and reviews on all aspects of the application of genomic and proteomic technologies to experimental and clinical cancer research. The journal’s scientific spectrum includes: (a) molecular causes of carcinogenesis, cancer progression and metastasis; (b) structural and functional aspects of genes in the cancer cell; (c) advances in genomic and proteomic technologies applicable to cancer research; (d) anticancer drug design and drug development. CGP will also accept abstracts and proceedings of scientific meetings for publication, following consideration and approval by the Editorial Board. A main aim of CGP is to ensure the prompt and confidential review, and rapid publication of original works and reviews, generally within 1-3 months from submission or 1-2 months from acceptance. International Institute of Anticancer Research Stanford University CGP is published bimonthly by the and is available online with HighWire Press: For more information please visit our websites www.iiar-anticancer.org and www.iiarjournals.org. Editorial Office

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A Selection of Recent Papers

Meta-data Analysis as a Strategy to Evaluate Individual and Common Features of Proteome Changes in Breast Cancer. O. ZAKHARCHENKO, (Stockholm; Uppsala, Sweden; Chelmsford, C. GREENWOOD, A. LEWANDOWSKA, U. HELLMAN, L. ALLDRIDGE, S. SOUCHELNYTSKYI UK; Olsztyn, Poland; Gold Coast, QLD, Australia)

Circadian Transcription Profile of Mouse Breast Cancer under LD and DD Conditions. E.-Y. OH, X. YANG , A. FRIEDMAN, C.M. ANSELL, J. DU- (Columbia, SC; St. Louis, MO, USA) QUITON, D.F. QUITON, P.A.WOOD, W.J.M. HRUSHESKY Interleukin 6/Interleukin 6 Receptor Interaction and its Role as a Therapeutic Target for Treatment of Cachexia and Cancer. U.H. WEIDLE, (Munich, Germany) S. KLOSTERMANN, D. EGGLE, A. KRÜGER A Biotin Label-based Antibody Array for High-content Profiling of Protein Expression. R. HUANG, W. JIANG, J. YANG, Y.Q. MAO, Y. ZHANG, (Norcross, GA, USA; Guangzhou, P.R. China) W. YANG, D. YANG, B. BURKHOLDER, R.F. HUANG. R.-P. HUANG TGF-β1 Interactome: Metastasis and Beyond. M. PERERA, C.S. TSANG, R.J. DISTEL, J.N. LACY, L. OHNO-MACHADO, V. RICCHIUTI, L.P. (Hong Kong, Hong Kong; Boston, MA; Ormond Beach, FL; SAMARANAYAKE, G.B. SMEJKAI, M.G. SMITH, A.J. TRACHTENBERG, W.P. KUO La Jolla, CA, USA)

Epigenetic Inactivation of the Placentally Imprinted Tumor Suppressor Gene TFPI2 in Prostate Carcinoma. T. RIBARSKA, M. INGENWERTH, (Düsseldorf, Germany) W. GOERING, R. ENGERS, W.A. SCHULZ Molecular Profiling Using Tissue Microarrays as a Tool to Identify Predictive Biomarkers in Laryngeal Cancer Treated with Radiotherapy. G. HOL- GERSSON, S. EKMAN, J. REIZENSTEIN, M. BERGQVIST, F. PONTÉN, M. UHLÉN, K. MAGNUSSON, P. JONNALAGADDA, A. ASPLUND, (Uppsala; Ørebro; Gävle; S. STRÖMBERG, A. LINDER, E. BLOMQUIST, M. LILJEHOLM, B. LÖDÉN, K. HELLSTRÖM, S. BERGSTRÖM Karlstad; Stockholm; Sweden; Mumbai, India)

Proteomic Study of Sera from Patients with Bladder Cancer: Usefulness of S100A8 and S100A9 Proteins. S. MINAMI, Y. SATO, T. MATSUMOTO, (Kanagawa, ) T. KAGEYAMA, Y. KAWASHIMA, K. YOSHIO, J.-I. ISHII, K. MATSUMOTO, R. NAGASHIO, I. OKAYASU Analysis of Protein Phosphorylation in Cisplatin-treated Human Cells Following Annexin V-based Separation and Multi-Antibody Screening. (Galway, Ireland) E. O’MEARA, S. CRUET-HENNEQUART, M.P. CARTY Identification of Melanoma Antigens Using a Serological Proteome Approach (SERPA). A. SUZUKI, A. IIZUKA, M. KOMIYAMA, M. TAKIKAWA, A. KUME, S. TAI, C. OHSHITA, A. KURUSU, Y. NAKAMURA, A. YAMAMOTO, N. YAMAZAKI, S. YOSHIKAWA, Y. KIYOHARA, Y. AKIYA- (; ; , Japan) MA DNA-based Assay for EPHB6 Expression in Breast Carcinoma Cells as a Potential Diagnostic Test for Detecting Tumor Cells in Circulation. B.P. FOX, (Pomona, CA, USA) R.P. KANDPAL Discovery of Serum Protein Biomarkers for Prostate Cancer Progression by Proteomic Analysis. J.A. ALRUWAILI, S.ET. LARKIN, B.A. ZEIDAN, (Hampshire, UK; Riyadh, Kingdom of Saudi Arabia; Boston, MA, USA) M.G. TAYLOR, C.N. ADRA, C.L. AUKIM-HASTIE, P.A. TOWNSEND Effects of Artesunate on Cytokinesis and G2/M Cell Cycle Progression of Tumour Cells and Budding Yeast. L. STEINBRÜCK, G. PEREIRA, (Heidelberg; Mainz, Germany) T. EFFERTH Use of NG2 (7.1) in AML as a Tumor Marker and its Association with a Poor Prognosis. K. PETROVICI, M. GRAF, K. HECHT, S. REIF, K. PFISTER, (Regensburg; Munich, Germany) H. SCHMETZER Contents continued

Salvage Chemotherapy for Ovarian Carcinoma Recurring During or After Consolidation Chemotherapy with Paclitaxel. Y. MIYOSHI, Y. UEDA, A. MORIMOTO, T. YOKOYAMA, S. MATSUZAKI, E. KOBAYASHI, KIMURA TOSHIHIRO, K. YOSHINO, M. FUJITA, T. ENOMOTO, KIMURA TADASHI (, , Japan) ...... 4613

Clinical Significance of BRAF Gene Mutations in Patients with Non-small Cell Lung Cancer. M. KOBAYASHI, M. SONOBE, T. TAKAHASHI, A. YOSHIZAWA, M. ISHIKAWA, R. KIKUCHI, K. OKUBO, C.-L. HUANG, H. DATE (, Japan) ...... 4619

Weekly Paclitaxel in Combination with Doxifluridine for Peritoneally Disseminated Gastric Cancer with Malignant Ascites. I. TAKEYOSHI, F. MAKITA, S. IWAZAKI, H. ISHIKAWA, S. KAKINUMA, Y. SATO, T. OHYA, K. NAKAGAMI, N. TOMIZAWA, M. IZUMI, I. KOBAYASHI, Y. TANAHASHI, J. KOBAYASHI, N. KAMOSHITA, S. KAWATE, Y. SUNOSE, I. SAKAMOTO, D. YOSHINARI, T. YAMADA, T. OKABE (; Shibukawa; Tatebayashi; ; Gunma; Fujiyoshida, Yamanashi; Usui; Koriyama, ; Ojiya, ; Ogawa, Saitama; Suto, , Japan) ...... 4625

The Prognostic Significance of HER2 Overexpression in Non-small Cell Lung Cancer. M. TAKENAKA, T. HANAGIRI, S. SHINOHARA, T. KUWATA, Y. CHIKAISHI, S. OKA, Y. SHIGEMATSU, Y. NAGATA, H. SHIMOKAWA, M. NAKAGAWA, H. URAMOTO, T. SO, F. TANAKA (, Japan) ...... 4631

Single-agent Paclitaxel in Advanced Anal Cancer after Failure of Cisplatin and 5-Fluorouracil Chemotherapy. A. ABBAS, E. NEHME, M. FAKIH (Buffalo, NY; Ann Arbor, MI, USA) ...... 4637

Clinical Characteristics and Treatment Outcomes of Hepatocellular Carcinoma with Inferior Vena Cava/Heart Invasion. Y.H. CHUN, S.H. AHN, J.Y. PARK, D.Y. KIM, K.-H. HAN, C.Y. CHON, S.J. BYUN, S.U. KIM (Seoul, South Korea) ...... 4641

Clinical Significance of Hypermethylation Status in NSCLC: Evaluation of a 30-Gene Panel in Patients with Advanced Disease. M. PESEK, M. KOPECKOVA, L. BENESOVA, A. MESZAROSOVA, P. MUKENSNABL, F. BRUHA, M. MINARIK (Pilsen; Prague, Czech Republic) ...... 4647

Growth Factors and Breast Tumors, Comparison of Selected Growth Factors with Traditional Tumor Markers. R. KUCERA, M. CERNA, A. NARSANSKA, S. SVOBODOVA, M. STRAKOVA, J. VRZALOVA, R. FUCHSOVA, I. TRESKOVA, T. KYDLICEK, V. TRESKA, L. PECEN, O. TOPOLCAN, P. PAZDIORA (Pilsen; Prague, Czech Republic) ...... 4653

Multi-center Phase II Study of FLOX for Advanced Colorectal Cancer Patients in Japan: SWIFT 3 Study. T. KATO, N. NAGATA, M. FUJII, H. TAKEMOTO, K. KONDO, Y. OKUYAMA, H. TOMINAGA, J. SAKAMOTO, H. MISHIMA (Hyogo; Kitakyushu; Osaka; ; ; Kyoto; , Japan) ...... 4657

Low Dihydropyrimidine Dehydrogenase Correlates with Prolonged Survival in Patients with Lung Adenocarcinoma Treated with 5-Fluorouracil. Y. SHINTANI, M. INOUE, Y. FUNAKOSHI, A. MATSUMURA, M. OHTA, H. MAEDA, M. OKUMURA (Osaka, Japan) ...... 4665

Index ...... 4673 * Reviews (pages 4083, 4359, 4369, 4387) Contents continued

Continuous Administration of EGFR-TKIs Following Radiotherapy after Disease Progression in Bone Lesions for Non-small Cell Lung Cancer. M. INOMATA, T. SHUKUYA, T. TAKAHASHI, A. ONO, Y. NAKAMURA, A. TSUYA, H. KENMOTSU, T. NAITO, H. MURAKAMI, H. HARADA, M. ENDO, N. YAMAMOTO (Shizuoka; ; Tokyo, Japan) ...... 4519

Hypersensitivity Reactions Associated with Platinum-containing Antineoplastic Agents for Thoracic Malignancies. M. TAMIYA, H. KUHARA, T. HIRASHIMA, Y. KONDO, M. SANTO, N. MORISHITA, H. SUZUKI, S. SASADA, N. OKAMOTO, M. KOBAYASHI, I. KAWASE (Osaka; Tokyo, Japan) ...... 4525

Carbonic Anhydrase IX, Hypoxia-inducible Factor-1 α, Ezrin and Glucose Transporter-1 as Predictors of Disease Outcome in Rectal Cancer: Multivariate Cox Survival Models following Data Reduction by Principal Component Analysis of the Clinicopathological Predictors. E.A. KORKEILA, J. SUNDSTRÖM, S. PYRHÖNEN, K. SYRJÄNEN (Turku, Finland) ...... 4529

Retrospective Study of Patients with Brain Metastases from Melanoma Receiving Concurrent Whole-brain Radiation and Temozolomide. N. DEVITO, M. YU, R. CHEN, E. PAN (Tampa, FL, USA) ...... 4537

Perioperative Non-tumorous Factors Associated with Survival in HCC Patients Who Underwent Hepatectomy. A. NANASHIMA, T. ABO, K. HAMASAKI, K. WAKATA, T. TOMINAGA, S. HIDAKA, H. TAKESHITA, T. NAGAYASU (, Japan) ...... 4545

Initial Experience with Hepatic Intraarterial Fotemustine and Interferon Alpha 2b Combination for Treatment of Liver Tumors. F.V. VITALE, P. ROMEO, E.M. DI MAGGIO, A. PARISI, E. MALAPONTE, S. CALÌ, F. VASTA, V. PANEBIANCO, D. ARCORIA, F. FERRAÙ (Taormina; Catania, Italy) ...... 4553

Prospective Study Evaluating the Plasma Concentrations of Twenty-six Cytokines and Response to Morphine Treatment in Cancer Patients. C. MAKIMURA, T. ARAO, H. MATSUOKA, M. TAKEDA, H. KIYOTA, J. TSURUTANI, Y. FUJITA, K. MATSUMOTO, H. KIMURA, M. OTSUKA, A. KOYAMA, C.K. IMAMURA, T. YAMANAKA, K. TANAKA, K. NISHIO, K. NAKAGAWA (Osaka, Sakai; Habikino, Osaka; Tokyo; , Japan) ...... 4561

Impact of Chemotherapy for Colorectal Cancer on Regulatory T-Cells and Tumor Immunity. K. MAEDA, S. HAZAMA, K. TOKUNO, S. KAN, Y. MAEDA, Y. WATANABE, R. KAMEI, Y. SHINDO, N. MAEDA, K. YOSHIMURA, S. YOSHINO, M. OKA (, Japan) ...... 4569

Radiofrequency Ablation for Intrahepatic Cholangiocarcinoma: Retrospective Analysis of a Single Centre Experience. A. GIORGIO, G. CALISTI, G. DE STEFANO, N. FARELLA, A. DI SARNO, F. AMENDOLA, U. SCOGNAMIGLIO, V. GIORGIO (Naples; Rome, Italy) ...... 4575

Trans-Arterial Chemoembolization of Metastatic Colorectal Carcinoma to the Liver Adopting DC Bead®, Drug-eluting Bead Loaded with Irinotecan: Results of a Phase II Clinical Study. C. ALIBERTI, G. FIORENTINI, P.C. MUZZIO, F. POMERRI, M. TILLI, S. DALLARA, G. BENEA (Padova; Pesaro; Ferrrara, Italy) ...... 4581

Docetaxel, Nedaplatin, and S-1 (DGS) Chemotherapy for Advanced Esophageal Carcinoma: A Phase I Dose- escalation Study. Y. TANAKA, K. YOSHIDA, S. OSADA, K. YAMAGUCHI, T. TAKAHASHI ( City, Gifu, Japan) ...... 4589

Long-term Survival Following Resection of Brain Metastases from Pancreatic Cancer. J. LEMKE, T.F.E. BARTH, M. JUCHEMS, T. KAPAPA, D. HENNE-BRUNS, M. KORNMANN (Ulm, Germany) ...... 4599

Comparison of Lymphangiogenesis between Primary Colorectal Cancer and Corresponding Liver Metastases. A. SCHOPPMANN, D. TAMANDL, B. HERBERGER, F. LANGLE, P. BIRNER, S. GELEFF, T. GRÜNBERGER, S.F. SCHOPPMANN (Vienna, Austria) ...... 4605 Contents continued on the preceding page Contents continued

Immunohistochemical Study of VEGF Expression in Oral Squamous Cell Carcinomas: Correlation with the mTOR-HIF-1 α Pathway. T. NARUSE, G. KAWASAKI, S. YANAMOTO, A. MIZUNO, M. UMEDA (Nagasaki, Japan) ...... 4429

Oxaliplatin, Irinotecan and Cetuximab in Advanced Gastric Cancer. A Multicenter Phase II Trial (Gastric- 2) of the Arbeitsgemeinschaft Medikamentose Tumortherapie (AGMT). E. WÖLL, R. GREIL, W. EISTERER, O. BECHTER, M.A. FRIDRIK, B. GRÜNBERGER, A. ZABERNIGG, B. MAYRBAURL, G. RUSS, M. DLASKA, P. OBRIST, J. THALER (Zams; Salzburg; Innsbruck; Linz; Vienna; Kufstein; Wels, Austria) ...... 4439

PCA3 Score vs PSA Free/Total Accuracy in Prostate Cancer Diagnosis at Repeat Saturation Biopsy. P. PEPE, F. ARAGONA (Catania, Italy) ...... 4445

The EMT Status in the Primary Tumor Does Not Predict Postoperative Recurrence or Disease-free Survival in Lung Adenocarcinoma. Y. CHIKAISHI, H. URAMOTO, F. TANAKA (Kitakyushu, Japan) ...... 4451

Correlation between IDH1 Gene Mutation Status and Survival of Patients Treated for Recurrent Glioma. S. LV, E. TEUGELS, J. SADONES, E. QUARTIER, M. HUYLEBROUCK, S. DU FOUR, M. LE MERCIER, O. DE WITTE, I. SALMON, A. MICHOTTE, J. DE GRÈVE, B. NEYNS (Brussels, Belgium) ...... 4457

Effect of Maintenance Chemotherapy with Gemcitabine and Paclitaxel on Recurrent Squamous Cell Carcinoma of the Bladder: A Case Report. Y. MIYATA, S.-I. WATANABE, H. TAKAHASHI, Y. SAGARA, T. MATSUO, K. OHBA, H. SAKAI (Nagasaki, Japan) ...... 4465

External Validation of a Laparoscopic-based Score to Evaluate Resectability for Patients with Advanced Ovarian Cancer Undergoing Interval Debulking Surgery. E. CHEREAU, V. LAVOUE, M. BALLESTER, C. COUTANT, F. SELLE, A. CORTEZ, E. DARAÏ, J. LEVEQUE, R. ROUZIER (Paris; Rennes, France) ...... 4469

Anaplastic Oligodendroglial Tumors Harboring 1p/19q Deletion Can Be Successfully Treated without Radiotherapy. Y. IWADATE, T. MATSUTANI, N. SHINOZAKI, N. SAEKI ( City, Chiba, Japan) ...... 4475

Synchronous Double Tumor of Breast Cancer and Gastrointestinal Stromal Tumor in a Patient with Neurofibromatosis Type 1: Report of a Case. H. TAKEUCHI, S. HIROSHIGE, K. HASHIMOTO, T. KUSUMOTO, Y. YOSHIKAWA, Y. MUTO (Beppu, Japan) ...... 4481

Clinical and Histopathological Profile of Primary or Secondary Osteosarcoma of the Jaws. F. ANGIERO, F. MOLTRASIO, G. CATTORETTI, M.G. VALENTE (Genoa; Milan, Italy) ...... 4485

The Cancer Stem Cell Antigens CD133, BCRP1/ABCG2 and CD117/c-KIT Are Not Associated with Prognosis in Resected Early-stage Non-small Cell Lung Cancer. E. HERPEL, K. JENSEN, T. MULEY, A. WARTH, P.A. SCHNABEL, M. MEISTER, F.J.F. HERTH, H. DIENEMANN, M. THOMAS, S. GOTTSCHLING (Heidelberg, Germany) ...... 4491

Evidence for Acute Vascular Toxicity of Cisplatin-based Chemotherapy in Patients with Germ Cell Tumour. K.-P. DIECKMANN, W.J. STRUSS, U. BUDDE (Hamburg, Germany) ...... 4501

Circulating CD95-ligand as a Potential Prognostic Marker for Recurrence in Patients with Synchronous Colorectal Liver Metastases. F.J.H. HOOGWATER, N. SNOEREN, M.W. NIJKAMP, A.C. GUNNING, W.J. VAN HOUDT, M.T. DE BRUIJN, E.E. VOEST, R. VAN HILLEGERSBERG, O. KRANENBURG, I.H.M. BOREL RINKES (Utrecht, Netherlands) ...... 4507

Dissection of Unsuspicious Para-aortic Lymph Nodes Does Not Improve Prognosis of Advanced Endometrial Carcinoma with Intra- or Extra-abdominal Metastasis. Y. UEDA, M. OKAZAWA, T. ENOMOTO, T. EGAWA-TAKATA, KIMURA TOSHIHIRO, K. YOSHINO, M. FUJITA, Y. OHTA, S. KAMIURA, KIMURA TADASHI (Osaka, Japan) ...... 4513 Contents continued on the preceding page Contents continued

Differential Cytotoxic Effects of Sodium Meta-arsenite on Human Cancer Cells, Dental Papilla Stem Cells and Somatic Cells Correlate with Telomeric Properties and Gene Expression. B.-G. JEON, B.M KUMAR, E-J. KANG, G.-H. MAENG, Y.-M. LEE, Y.-S. HAH, S.-A OCK, D.-O. KWACK, B.-W. PARK, G.-J. RHO (Jinju, Republic of Korea) ...... 4315

Genome-wide Identification of Chemosensitive Single Nucleotide Polymorphism Markers in Gastric Cancer. Y.J. HA, S.N. YOON, Y.J. JEON, D.H. CHO, S.A. ROH, B.S. KIM, H.J. KIM, S.Y. KIM, Y.S. KIM, J.C. KIM (Seoul; Daejeon; Gyeoggi-Do, Republic of Korea) ...... 4329

De-alcoholization of Paclitaxel Injection for Clinical Application. I. TSUKIYAMA, M. TAKEUCHI, F. ABE, T. KISI, H. SAITO, Y. OBAYASHI, K. WATANABE, A. WAKATSUKI, K.-I. MIYAMOTO, T. HASEGAWA (Aichi-gun, Aichi; , Ishikawa, Japan) ...... 4339

Modulation of PGE2-induced EP4 Expression on Snail Signaling and the Impact on Epithelial-Mesenchymal Transition: Significance of EP4 Antagonism. H.N. KIM, N.K. NARAYANAN, S. LASANO, B. NARAYANAN (Tuxedo, NY, USA) ...... 4347

Clinical Studies * Review: Impact of Cyclooxygenase-2 in Breast Cancer. F. HOELLEN, K. KELLING, C. DITTMER, K. DIEDRICH, M. FRIEDRICH, M. THILL (Luebeck; Krefeld, Germany) ...... 4359

* Review: Estrogen Pathway Polymorphisms and Mammographic Density. I. DUMAS, C. DIORIO (Quebec City, QC, Canada) ...... 4369 * Review: Targeting AKT Protein Kinase in Gastric Cancer. K. ALMHANNA, J. STROSBERG, M. MALAFA (Tampa, FL, USA) ...... 4387

Feasibility of Image-guided Radiotherapy Based on Tomotherapy for the Treatment of Locally Advanced Anal Carcinoma. N.P. NGUYEN, J. VOCK, T. SROKA, R. KHAN, S. JANG, A. CHI, M. BETZ, L. EWELL, D. COHEN, R.P. VO, M. MILLS, V. VINH-HUNG (Galveston, TX, USA; Bern; Geneva, Switzerland) ...... 4393

Primary Peritoneal Carcinoma in Complete Remission: a Case Report. J. RAMOS, K.S. GUNTURU, S. KRISHNAMOORTHY, B. KENNEY, M.W. SAIF (New Haven, CT; New York, NY, USA) ...... 4397 KIP1 Loss of p27 Expression in Fully-staged Node-negative Breast Cancer: Association with Lack of Hormone Receptors in T1a/b, but not T1c Infiltrative Ductal Carcinoma. D. MIRCHANDANI, D.F. ROSES, G. INGHIRAMI, A. ZELENIUCH-JACQUOTTE, J. CANGIARELLA, A. GUTH, R.A. SAFYAN, S.C. FORMENTI, M. PAGANO, F. MUGGIA (New York, NY, USA) ...... 4401

Triple Induction Chemotherapy and Chemoradiotherapy for Locally Advanced Esophageal Cancer. A Phase II Study. W. EISTERER, A. DE VRIES, D. KENDLER, B. SPECHTENHAUSER, A. KÖNIGSRAINER, H. NEHODA, I. VIRGOLINI, P. LUKAS, O. BECHTER, E. WÖLL, D. ÖFNER (Innsbruck; Feldkirch; Kufstein; St. Johann; Zams; Salzburg, Austria; Tübingen, Germany) ...... 4407

Intraepidermal Nerve Fibre Density in Cancer Patients Receiving Adjuvant Chemotherapy. M.J. KOSKINEN, A.-L. KAUTIO, M.L. HAANPÄÄ, H.K. HAAPASALO, P.-L. KELLOKUMPU-LEHTINEN, T. SAARTO, A.J. HIETAHARJU (Tampere; Helsinki, Finland) ...... 4413

First Study of Oral Artenimol-R in Advanced Cervical Cancer: Clinical Benefit, Tolerability and Tumor Markers. F. H. JANSEN, I. ADOUBI, K.C. COMOE, T. DE CNODDER, N. JANSEN, A. TSCHULAKOW, T. EFFERTH (Abidjan, Côte d’Ivoire; Liège, Belgium; Mainz, Germany) ...... 4417

Impaired Bone Microenvironment: Correlation between Bone Density and Presence of Disseminated Tumor Cells. B. KRAEMER, R. ROTHMUND, M. BANYS, N. KRAWCZYK, E.-F. SOLOMAYER, C. MACK, D. WALLWIENER, T. FEHM (Tuebingen; Hamburg; Homburg, Germany) ...... 4423 Contents continued on the preceding page Contents continued

Prognostic Significance of N-Cadherin Expression in Oral Squamous Cell Carcinoma. M. DI DOMENICO, G.M. PIERANTONI, A. FEOLA, F. ESPOSITO, L. LAINO, A. DE ROSA, R. RULLO, M. MAZZOTTA, M. MARTANO, F. SANGUEDOLCE, L. PERILLO, L. D’ANGELO, S. PAPAGERAKIS, S. TORTORELLA, P. BUFO, L. LO MUZIO, G. PANNONE, A. SANTORO (Naples; Foggia; Bari, Italy; Ann Arbor, MI, USA) ...... 4211 ® Circulating Tumor Cells and “Suspicious Objects” Evaluated Through CellSearch in Metastatic Renal Cell Carcinoma. A. GRADILONE, R. IACOVELLI, E. CORTESI, C. RAIMONDI, W. GIANNI, C. NICOLAZZO, A. PETRACCA, A. PALAZZO, F. LONGO, L. FRATI, P. GAZZANIGA (Rome, Italy) ...... 4219 κ Resveratrol Reduces TNF- α- induced U373MG Human Glioma Cell Invasion through Regulating NF- B Activation and uPA/uPAR Expression. J. RYU, B.M. KU, Y.K. LEE, J.Y. JEONG, S. KANG, J. CHOI, Y. YANG, D.H. LEE, G.S. ROH, H.J. KIM, G.J. CHO, W.S. CHOI, N. KIM, S.S. KANG (Jinju; Gimhae; Seoul, South Korea) ...... 4223

Quantitative Structure-Activity Relationship (QSAR) Analysis of Tumor-specificity of 1,2,3,4- Tetrahydroisoquinoline Derivatives. Y. UESAWA, K. MOHRI, M. KAWASE, M. ISHIHARA, H. SAKAGAMI (Tokyo; ; Saitama, Japan) ...... 4231

Cytoskeleton Network and Cellular Migration Modulated by Nuclear-localized Receptor Tyrosine Kinase ROR1. H.-C. TSENG, H.-W. KAO, M.-R. HO, Y.-R. CHEN, T.-W. LIN, P.-C. LYU, W.-C. LIN (Hsinchu; Taipei, Taiwan, ROC) ...... 4239

Novel Cytotoxic Isolated from Jamaican Hyptis verticillata jacq Induces Apoptosis and Overcomes Multidrug Resistance. Y. WHITE, T. HAMADA, M. YOSHIMITSU, M. NAKASHIMA, M. HACHIMAN, T. KOZAKO, K. MATSUSHITA, K. UOZUMI, S. SUZUKI, H. KOFUNE, T. FURUKAWA, N. ARIMA (; Fukuoka, Japan) ...... 4251

IGF1Ec Expression in MG-63 Human Osteoblast-like Osteosarcoma Cells. A. PHILIPPOU, A. ARMAKOLAS, Z. PANTELEAKOU, N. PISSIMISSIS, A. NEZOS, A. THEOS, M. KAPARELOU, N. ARMAKOLAS, S.G. PNEUMATICOS, M. KOUTSILIERIS (Athens, Greece) ...... 4259

Mutations of Mitochondrial DNA as Potential Biomarkers in Breast Cancer. F.F. CAI, C. KOHLER, B. ZHANG, W.J. CHEN, Z. BAREKATI, H.S.P. GARRITSEN, P. LENNER, P. TONIOLO, J.J. ZHANG, X.Y. ZHONG (Basel; Lausanne, Switzerland; Braunschweig, Germany; Umeä, Sweden; Hefei, Anhui, PR China) ...... 4267

Differential CD133 Expression Pattern during Mouse Colon Tumorigenesis. V. ARENA, E. CAREDDA, V. CUFINO, E. STIGLIANO, F. SCALDAFERRI, A. GASBARRINI, A. CITTADINI, A. SGAMBATO (Rome, Italy) ...... 4273

The Expression of Ki-67, but Not Proliferating Cell Nuclear Antigen, Predicts Poor Disease Free Survival in Patients with Adenocarcinoma of the Lung. S. OKA, H. URAMOTO, H. SHIMOKAWA, T. IWANAMI, F. TANAKA (Kitakyushu, Japan) ...... 4277

Synergism from the Combination of Oxaliplatin with Selected Phytochemicals in Human Ovarian Cancer Cell Lines. N.M. YUNOS, P. BEALE, J.Q. YU, F. HUQ (Kepong, Malaysia; Sydney, NSW, Australia) ...... 4283

Investigation of Cell Death Induced by N-Methyl- N-Nitrosourea in Cell Lines of Human Origin and Implication of RNA Binding Protein Alterations. A. KORYLLOU, M. PATRINOU-GEORGOULA, A. DIMOZI, S.A. KYRTOPOULOS, V. PLETSA (Athens, Greece) ...... 4291

Association of p53 and CDKN1A Genotypes with Endometriosis. T.-H. YING, C.-J. TSENG, S.-J. TSAI, S.- C. HSIEH, H.-Z. LEE, Y.-H. HSIEH, D.-T. BAU (Taichung, Taiwan, ROC) ...... 4301

Human Brain Endothelial Cell-derived COX-2 Facilitates Extravasation of Breast Cancer Cells Across the Blood-brain Barrier. K.Y. LEE, Y.-J. KIM, H. YOO, S.H. LEE, J.B. PARK, H.J. KIM (Goyang; Daejeon, Republic of Korea) ...... 4307 Contents continued on the preceding page Contents continued

Characterization of T-Cell Memory Phenotype after In Vitro Expansion of Tumor-infiltrating Lymphocytes from Melanoma Patients. J. ZHOU, P. NAGARKATTI, Y. ZHONG, M. NAGARKATTI (Columbia, SC, USA) ...... 4099

Effects of Artemisinin Dimers on Rat Breast Cancer Cells In Vitro and In Vivo . N.P. SINGH, H.C. LAI, J.S. PARK, T.E. GERHARDT, B.J. KIM, S. WANG, T. SASAKI (Seattle, WA, USA) ...... 4111

Histone-modifier Gene Expression Profiles Are Associated with Pathological and Clinical Outcomes in Human Breast Cancer. N. PATANI, W.G. JIANG, R.F. NEWBOLD, K. MOKBEL (London; Cardiff, Wales, UK) ..... 4115

PINCH mRNA Overexpression in Colorectal Carcinomas Correlated with VEGF and FAS mRNA Expression. Z.Y. ZHANG, Y.F. TIAN, Y.Y. WANG, L.J. ZHANG, Z.R. ZHAO, X.F. SUN (Tangshan; Shijiazhuang, PR China; Linköping, Sweden) ...... 4127

ERCC1 Expression and Outcomes in Head and Neck Cancer Treated with Concurrent Cisplatin and Radiation. M. HAYES, C. LAN, J. YAN, Y. XIE, T. GRAY, R.H. AMIRKHAN, J.E. DOWELL (Miramar Beach, FL; Modesto, CA; Dallas, TX, USA) ...... 4135

Treatment of Bone Metastasis in Prostate Cancer: Efficacy of a Novel Poly bis phosphonate. F. DAUBINE, R. LE BOT, M. MÁRQUEZ, S. NILSSON, T. SCHRÖDER, A.R. HOLMBERG (Nantes, France; Stockholm, Sweden) ...... 4141

REG4, NEIL2, and BIRC5 Gene Expression Correlates with Gamma-radiation Sensitivity in Patients with Rectal Cancer Receiving Radiotherapy. T. KOBUNAI, T. WATANABE, T. FUKUSATO (Tokyo, Japan) ...... 4147

Overexpressed HER2 in NSCLC Is a Possible Therapeutic Target of EGFR Inhibitors. N. ISE, K. OMI, D. NAMBARA, S. HIGASHIYAMA, K. GOISHI (Tokyo; Ehime, Japan) ...... 4155

Acridine Orange Inhibits Pulmonary Metastasis of Mouse Osteosarcoma. H. SATONAKA, K. KUSUZAKI, K. AKEDA, M. TSUJII, T. IINO, T. UEMURA, T. MATSUBARA, T. NAKAMURA, K. ASANUMA, A. MATSUMINE, A. SUDO (Tsu City, Mie; Kyoto City, Kyoto, Japan) ...... 4163

Effects of Inorganic and Organic Arsenic Compounds on Growth and Apoptosis of Human T-Lymphoblastoid Leukemia Cells. E. HIKITA, M. ARAI, S. TANAKA, K. ONDA, H. UTSUMI, B. YUAN, H. TOYODA, T. HIRANO (Hachioji; Tokyo, Tokyo; , Kanagawa, Japan) ...... 4169

Mutational Screening of RET, HRAS, KRAS, NRAS, BRAF, AKT1, and CTNNB1 in Medullary Thyroid Carcinoma. H.-J. SCHULTEN, J. AL-MAGHRABI, K. AL-GHAMDI, S. SALAMA, S. AL-MUHAYAWI, A. CHAUDHARY, O. HAMOUR, A. ABUZENADAH, M. GARI, M. AL-QAHTANI (Jeddah, Saudi Arabia) ..... 4179

Inactivated Orf virus ( Parapoxvirus ovis ) Induces Antitumoral Activity in Transplantable Tumor Models. H.- H. FIEBIG, A. SIEGLING, H.-D. VOLK, A. FRIEBE, P. KNOLLE, A. LIMMER, O. WEBER (Freiburg; Wuppertal; Bonn, Germany) ...... 4185

Expression of the Apoptosis-related Genes Bcl-2 and p53 in Clinical Samples from Endometrial Carcinoma Patients. I. GONZÁLEZ-RODILLA, V. VERNA, A.-B. MUÑOZ, J. ESTÉVEZ, M. BOIX, J. SCHNEIDER (Santander, Spain) ...... 4191 positive Yeast-derived Beta-(1-3),(1-6)-D-glucan Induces Up-regulation of CD86 on Dectin-1- Human B-Lymphoma Cell Lines. U. HARNACK, U. KELLERMANN, G. PECHER (Berlin, Germany) ...... 4195

Thioridazine Induces Apoptosis of Multidrug-resistant Mouse Lymphoma Cells Transfected with the Human ABCB1 and Inhibits the Expression of P-Glycoprotein. G. SPENGLER, J. MOLNAR, M. VIVEIROS, L. AMARAL (Szeged, Hungary; Lisboa, Portugal; Brussels, Belgium) ...... 4201

Reliability of Direct Sequencing of EGFR: Comparison between Cytological and Histological Samples from the Same Patient. P. BRUNO, S. MARIOTTA, A. RICCI, E. DURANTI, D. SCOZZI, A. NOTO, R. MANCINI, E. GIARNIERI, M.R. GIOVAGNOLI (Rome, Italy) ...... 4207 Contents continued on the preceding page