Redacted for Privacy Kenton Lee Chambers

AN ABSTRACT OF THE THESIS OF

RONALD JAY TYRL for the DOCTOR OF PHILOSOPHY (Name) (Degree) in BOTANY (Systematic Botany) presented on December 10, 1969 (Major) (Date) Title:ORIGIN AND DISTRIBUTION OF POLYPLOID ACHILLEA

(COMPOSITAE) IN WESTERN NORTH AMERICA Abstract approved:Redacted for privacy Kenton Lee Chambers

A cytogeographical study was made of populations of tetraploid (n=18) and hexaploid (n=27) Achillea millefolium (Compositae- Anthemideae) from the Pacific Coast states.There is a complex distributional pattern, involving overlapping ranges, mixed populations, migration of one form into areas occuplied by the other, pentaploid hybrids, and septaploid and octoploid plants.The distributions of the coastal tetraploids in the areas of southwestern Oregon, northwestern California, and northwestern Washington, are corre- lated with the distinctive geological regions of the Klamath and the Olympic Mountains.Tetraploid plants of these areas frequently produce unreduced micros pores with a chromosome number ofn=36. On the basis of these observations, several inferences are advanced.First, the distributions of the two chromosome forms are not static, but rather dynamic, with man being instrumental in dispersal and establishment of Achillea populations.Second, the North American hexaploid is indigenous, having its origins in the tetraploid through the union of unreduced or tetraploid gametes with normal diploid gametes.Evidence for this hypothesis is found in the reproductive barriers between American and Eurasian hexaploids; the meiotic configurations of the pentaploid hybrids; the existence of mixed populations in which single hexaploid plants occur among tetraploids that are producing unreduced gametes; and the morphological and ecological similarities indicative of an autoploid system of evolution. From the distributional patterns detected, it is believed that the tetraploid, established during Tertiary times in the Klamath and Olympic Mountains, gave rise to the hexaploids at the geological margins of these areas. Due to these observations and the complete intergrading morphological variation characterizing the genus, it is suggested that Achillea in western North America is adequately and meaningfully described as a single species, highly polymorphic and with two or more ploidy levels. Origin and Distribution of Polyploid Achillea (Compositae) in Western North America

by Ronald Jay Tyr'

A THESIS submitted to Oregon State University

in partial fulfillment of the requirements for the degree of Doctor of Philosophy June 1970 APPROVED: Redacted for privacy Professor of Botany in charge of major

Redacted for privacy

Head a/Department tBotany and Plant Pathology

Redacted for privacy

Dean 6f Graduate School .1.1,

Date thesis is presented December 10, 1969 Typed by Donna L. Olson for Ronald Jay Tyrl ACKNOWLEDGMENT

I wish to express my thanks to Dr. Kenton L. Chambers for his guidance and encouragement during my graduate work. Appreciated are the suggestions and constructive criticisms made during the research and writing, especially those words and phrases which so often transformed my awkwardly phrased thoughts into gems of wis- dom. Thanks are also due Mrs. La Rea Dennis Johnston, from whom I have learned much of taxonomy. I gratefully acknowledge those individuals, who searching in vain for the elusive diploids, collected plants and achenes for me -- Dr. James Estes, Mr. Wayland Ezell, Mr. Alan Legge, and Mr. Robert Carr. Appreciated also is Dr. Frank Smith, who has contri- buted time and thought to the research compiled in this thesis. To Lynda, my wife, a special thanks. Adept at distinguishing Achillea from Daucus and Anaphalis, she was invaluable on collect- ing trips.The detection of the heretofore unreported hexaploids in interior southwestern Oregon may be attributed to her persistence. This research was aided in part by The Northwest Scientific Association, the Department of Botany and Plant Pathology at Oregon State University and a Grant-in-Aid of Research from the Society of the Sigma. Xi. TABLE OF CONTENTS

Page

INTRODUCTION 1

MATERIALS AND METHODS 5

RESULTS AND OBSERVATIONS 7

DISCUSSION 19

Distributional Patterns 19 C ytogenetic Relationships 24 Cytogeographic Patterns 27 Taxonomic Treatment 29

BIBLIOGRAPHY 37

APPENDIX I 42 LIST OF FIGURES

Figure Page

1. Distribution of polyploid Achillea in western North America as determined by previous investigators. 2

2. Distribution of Achillea in Alaska, southwestern British Columbia, and western Washington. 8

3. Distribution of Achillea in western Oregon. 9

4. Distribution of Achillea in the Klamath Moun- tains of southwestern Oregon and northwestern California. 10

5. Distribution of Achillea in central and southern California. 11

6. Photomicrographs of reduced chromosome complements of Achillea; metaphase of first mitotic division in microspores. 15

7. Photomicrographs of unreduced microspores of Achillea; metaphase of first mitotic division in microspores. 15

8. Photomicrographs of unreduced microspores among reduced spores in anther sacs of tetraploid and hexaploid Achillea. 16

9. Photomicrograph of tetraploid Achillea microspore quartets; two with only three microspores. 16

10. Distribution of tetraploid Achillea plants producing unreduced microspores. 17

11. Comparison of internal pollen grain diameters of Achillea. 34 ORIGIN AND DISTRIBUTION OF POLYPLOID ACHILLEA (COMPOSITAE) IN WESTERN NORTH AMERICA

INTRODUCTION

The Achillea millefolium L. complex (Compositae-Anthemideae) is a polyploid complex composed of diploids, tetraploids, hexaploids, and octoploids with a base chromosome number of nine.The group is cosmopolitan in the northern hemisphere having greatest diversity in southeastern Europe and southwestern to central Asia.In North America, tetraploids (211=36) and hexaploids (2n=54) have been reported to occur. From chromosome counts by Clausen, Keck & Hiesey (1938, 1940, 1948), Turesson (1949), Lawrence (1947), Ehrendorfer (1952b), Ehrle (1958), and Mulligan & Bassett (1959), a general distributional map was developed (Figure 1).The tetraploid was thought to be continental except for the coastal areas of northwestern Washington and southwestern Oregon.In these two regions the hexaploids, believed to occupy only the coastal strip from northern Alaska to Baja California, were thought to be completely replaced by the tetraploids.In central California, hexaploids were reported far inland, while tetraploids were found in the marshlands of the San Francisco Bay area. On the basis of pollen grain diameter as an indicator of chromosome number, Mulligan & Bassett believed Figure 1.Distribution of polyploid Achillea in western North America as determined by previous investigators (see text).Circles, tetraploids (n=18); triangles, hexaploids (n=27); square, octoploid (n=36), the hexaploids to occur also along the Arctic Coast of Alaska and Canada, while the scattered 6x plants of the Canadian Atlantic Coast were thought to be recent introductions from Europe. Interpreting their counts, Lawrence, Clausen, and Ehrendorfer emphasized a relatively simple pattern of distribution, the tetraploids and hexaploids replacing one another in different areas and very infrequently occurring together.However, following a study of Achillea in western Oregon (1967, 1969), I reported the distributional pattern of the two ploidy levels to be more complex than previously described.This involved the discovery of overlapping ranges of 4x and 6x populations, the occurrence of mixed populations, and the presence of disjunct populations of one form in areas occupied by the other. A naturally occurring pentaploid hybrid showing 18 II's and 9 Its was found, which could not be distinguished morphologically from the presumed parents.The northern limits of the coastal tetraploids in southwestern Oregon coincide with the northern limits of the Klamath Mountains.Plants of this geologically distinctive area, especially those at its geographical margins, produce a large number of viable unreduced pollen grains.These data suggest that the hexaploids under study are indigenous to North America and originated by autopolyploidy from earlier existing tetraploids. As a result of these contrasting distributional patterns, in- vestigations of tetraploid and hexaploid Achillea were continued.The objectives of the study were three.First was to develop a broader and more accurate distributional pattern for the Pacific. Coast states.Three areas were of major interest.One was southern California and a determination of whether the coastal hexaploids continue southward into Baja California or whether the tetraploids occur on the coast.The second area was northwestern California to clarify the southern distributional limits of the coastal tetraploids, noting possible correlations with the Klamath Mountains as was found on the north.The third area of interest was the distribution of tetraploids and hexaploids on the Olympic Peninsula and surrounding areas. A second objective was to determine whether unreduced pollen grains, and 6x or 5x plants, are being produced at the southern limits of the coastal tetraploids in northwestern California, on the Olympic Peninsula, and in the area from which a single octoploid plant has been reported (Lawrence, 1947). The final objective was to develop a theory as to the origin(s) of the coastal hexaploids in western North America. 5

MATERIALS AND METHODS

Distribution of the two chromosome forms of Achillea was determined by chromosome counts of 587 populations in Alaska, British Columbia, Washington, Oregon, and California (Appendix I; Tyrl, 1967).Population samples normally consisted of material from two or three plants and, unless otherwise noted, were in the general proximity of the highway right of way, i.e., the roadside embankments, ditches, or adjacent fence rows and fields.Each locality was assigned an accession number and located by range, township, and section.Cities and waterways provided reference points, and mileages were calculated from the city limit signs or the bridge centers. Heads in various stages of flowering were fixed in a modified Carnoy's solution (chloroform, 95% ethanol, glacial acetic acid; 6:3:1) for a minimum of 24 hours, washed in 70% ethanol, stained in bulk using Snow's carmine stain (Snow, 1963) for 24 hours at 60°C., and then washed again in 70% ethanol. Antherswere then mounted and squashed in Hoyer's medium. Achenes were germinated on moist filter paper in Petri dishes.Root tips were then treated with 0.2% aqueous colchicine for three hours at 6°C. ,fixed in 95% ethanol and glacial acetic acid (3: 1), washed in 70% ethanol, stained in Snow's carmine, and washed again. 6 Counts were made primarily from squash mounts of microspores, in which the chromosomes could readily be distinguished at metaphase in the first mitotic division (Figure 6).Microsporocyte meiosis was examined only when abnormalities were noted in the microspore mitosis.Counts were obtained from three or four microspores per plant. 7

RESULTS AND OBSERVATIONS

Chromosome numbers occurring in each examined population are presented in Figures 2-5 and Appendix I.In general, the hexaploids occupy habitats on the coasts of Washington and Oregon, while the tetraploids occur in the interior except for the coastal regions of the Klamath and Olympic Mountains.In Washington, the Olympic Mountains cover the Olympic Peninsula, extending south to the middle of Grays Harbor and Mason Counties and the upper drainages of Chehalis River tributaries (Campbell, 1953).Tetraploids occupy the Peninsula, also being present on Vancouver Island and adjacent islands.The hexaploids occur farther north in the Queen Charlotte Islands (Taylor & Mulligan, 1968) and in the Chehalis River drainage to the south.In southwestern Oregon and northwestern California, the transition between 4x and 6x plants occurs between the Coquille

Riverand.Coos Bay on the north and in the Humboldt Bay-Trinity River region on the south.These areas are considered to be the geological limits of the Klamath Mountains, a general term describ- ing a complex of mountain ranges in southwestern Oregon and northern California (Baldwin, 1945, 1964). It appears that the hexaploids continue southward along the Pacific Coast from the southern limits of the Klamath Mountains into Mexico (Figure 4).In central California, the hexaploids are present 8

CAMPBELL RIVER REGION, VANCOUVER I.

Vancouver Nanai mo

BRITISH COLUMBIA

W04 WASHINGTON 607A *603 411 VANCOUVER ISLAND

*6 0 1 Tir611,

CD 2C Hoh R 58 OLYMPIC "m MOUNTAINS COD

Seatt5I

A*378 AA

Grays Harbo

Chehalis R. Mt. Rainier

590

Figure 2.Distribution of Achilles in Alaska, southwestern British Columbia and western Washington.Circles, tetraploid populations (n=18); triangles, hexaploid populations (n=27); squares, mixed populations; stars, pentaploid plants (211=45). 9

Cowlitz R.

Columbia R.

Nestucca R.

II Ri chl and

Willamette R. The Dallas

COLUMBIA RIVER IN EASTERN OREGON MD WASHINGTON

Yaqui na R.

,56

Al sea R.

Si slaw

Umpqua R.

s Bay

Coqui 1 le R. Roseburg

Figure 3.Distribution of Achillea in western Oregon.Circles, tetraploid populations (n=18); triangles, hexaploid populations (n=27); squares, mixed populations. 10

AA, et v\--N. Fork Umpqua Coquille R. Roseburg

ALtA../-t, AA S. Fork repcitoR.

4/59A ale Rogue R. S.

445 A.II! III nois R. 428 AA

Medford \--\''lA 512 Klamath Falls t A OREGON CALIFORNIA

AACrescent City

Shasta R.

Klamath R. 1

Scott R.

ity R.

Eel R.

Figure 4,Distribution of Achillea in the Klamath Mountains of southwestern Oregon and northwestern California,Circles, tetraploid populations (n=18); triangles, hexaploid populations (n=27); squares, mixed populations; stars, pentaploid plants (2n°45). 11

S. Fork Eel R.

Sacramento R.

San Joaquin R.

11a Fresno AAA W---332,.. -331 7.. 04 .. ir... ../.

San Luis Obispo

Barstow

os Angeles

*201 pn Diego

Figure 5.Distribution of Achillea in central arxd southern California.Circles, tetraploid populations (n=18); triangles, hexaploid populations (n=27); stars, pentaploid plants (2n=45). 12 throughout the interior, occurring in the Sierra Nevada foothills. For example, accession 331 from Squaw Valley in Fresno County is at an elevation of 1650 ft.The tetraploids also appear to continue southward, being found in the San Bernardino Mountains and, as reported by Lawrence, in the Palomar Mountains,They were not found in the marshlands of the San Francisco Bay area, where they had been reported by Ehrendorfer.However, tetraploid populations (229, 244, 246, 247, 255) were found on the California coast among hexaploids. Twelve pentaploid plants, 2n=45, were discovered. Seven (85-4, 201-2, 445-1, 601-1, 603-1, 611-2, 612-1) were among tetraploid plants; two (262-4, 481-1) were among hexaploids; and three (332-1, 378-1, 459-1) were growing alone but with tetraploid and hexaploid populations nearby.Collected in the course of random samp- ling, the plants were not morphologically distinctive.Usually there were 18 bivalents and 9 univalents at the first meiotic metaphase in the microsporocytes. However, in plants 378-1 and 481-1 there were consistently one or two trivalents per cell, and in plant 611-2 more than nine univalents occurred. Chromosome numbers in the microspores of all pentaploids at the first mitotic division ranged from 18 to 27, suggesting random segregation of the univalents. Almost all of the pollen grains appeared to be viable, the cytoplasm staining readily. Numerous achenes were collected from these 5x 13 plants in nature.Seventeen were germinated and root tip chromosome counts made. Somatic numbers obtained were:36(4), 37, 38(2), 39(2), 40, 42, 43(2), 44, 46, 48, and 54.Other 5x progeny have been established in greenhouse facilities at Oregon State Univer- sity for subsequent examinations of meiosis. Two morphologically indistinguishable plants with different chromosome numbers were found in hexaploid population 399 from southwestern British Columbia. One plant was a septaploid (2n=63). In each of the six microsporocytes that could be analyzed clearly at the first meiotic metaphase, there were 31 bivalents, each with one chiasma, and one univalent.Chromosome numbers in the microspores were 31, 32, and 33.The microspores were judged to be viable on the basis of cytoplasmic staining and morphology.The other plant proved to be octoploid (2n=72).Metaphase I configurations in each of four cells showed 36 bivalents with one chiasma each. Cells at anaphase I and at post-meiotic metaphase contained 36 chromosomes and the microspores appeared to be viable.It was also noted that one of the 12 hexaploid plants collected was producing viable unreduced pollen grains in abundance.In some anthers, there were 40 to 50 among the 150 to 200 reduced spores. At the first mitotic metaphase in the microspores, 54 chromosomes rather than the normal 27 were observed. The phenomenon of unreduced gamete production was not 14 uncommon in Achillea.Eight other hexaploid plants from throughout the study area produced unreduced spores (Figure 7b).Tetraploids were also observed to have unreduced microspores with36 chromosomes among the reduced spores with 18 (Figure 7a).Only three microspores were produced following meiosis in some microsporocytes (Figure 9).Studies to determine the nature of the irregularity were not carried out at this time, though it is known thataberrations in synapsis, chromosome reproduction, spindle action, and cell wall formation characterize the complex [ See. Ehrendorfer (1959b) for a detailed discussion of such phenomena],. These 4x and 6x spores were easily distinguished by their large diameters, about twice normal size, and numbered from two to twenty among 250-300 reduced spores in each anther sac (Figure 8).Based on cytoplasmic staining and morphology, the grains appeared to be viable.The plants producing them were morphologically indistinguishable from other tetraploids and hexaploids which were producing only reduced microspores. Fourteen percent of the 480 tetraploid plants examined were producing unreduced microspores. However, tetraploids exhibiting this phenomenon were centered in several areas (Figure 10):(1) the Klamath Mountains of southwestern Oregon and northwestern Cali- fornia with 23. 5% of the sampled plants producing 4x spores; (2) coastal Coos County in Oregon, especially the Coquille River valley 15

Figure 6. Photomicrographs of reduced chromosome complements of Achillea; metaphase of first mitotic division in microspores. X 1250.(A) Tetraploid (n=18).(B) Hexaploid (n=27). Figure 7. Photomicrographs of unreduced microspores of Achillea; metaphase of first mitotic division in microspores. X 1000.(A) Unreduced tetraploid (n=36).(B) Unreduced hexaploid (n=54). 16

Figure 8. Photomicrographs of unreduced microspores among reduced spores in anther sacs of tetraploid and hexaploid Achillea. X 400.(A) Tetraploid.(B) Hexaploid. Figure 9. Photomicrograph of tetraploid Achillea microspore quartets; two with only three microspores. 17

Figure 10.Distribution of tetraploid plants producing unreduced microspores 18 with 43%; (3) the Rogue and Umpqua River valleys of interior southwestern Oregon with 19. 2 %; (4) the Klamath, Salmon, and Trinity River valleys of northwestern California with 24.6%; (5) the eastside of the Puget Sound basin in northwestern Washington with 24.2%; and (6) isolated populations in the Alsea River valley, the Columbia River valley, and at the southern limits of the Olympic Mountains, where all plants examined were producing some 4x spores. 19

DISCUSSION

Distributional Patterns

Previous interpretations of the distribution of tetraploid and hexaploid Achillea in western North America indicated that the two forms were more or less distinct, although the apparent lack of ecological barriers between them was noted.The additional counts reported here clarify the pattern, revealing that the distribution of tetraploid and hexaploid Achillea are overlapping rather than distinct. It is a complex pattern which may be related to the cytogenetic and cytogeographical history of the genus. This complexity of 4x-6x distribution is illustrated by the discovery of numerous hexaploid populations in areas previouslythought to contain only tetraploids.Ehrendorfer (1952b) described northwest Washington as a region where the tetraploids extended from the interior westward onto the Olympic Peninsula, the hexaploids occurring farther north and south along the coast.However, hexaploids were commonly found on the east side of the Puget Sound basin among tetraploids (Figure 2).Also unexpected was the occurrence of the supposedly coastal hexaploids at 4400 feet elevation in the Cascade Mountains near Mt. Rainier.Lawrence (1947) had reported a hexaploid 50 miles inland from the coast at Chehalis in Lewis

County.However, these populations (589, 590) were approximately 20 65 miles farther Eastor about 115 miles from the coast. A third area of unexpected hexaploids exists in southwestern Oregon.Hexa- ploids occurred abundantly in theUmpqua and Illinois River drainages, areas previously reported to be regions of tetraploiddistribu- tion.Hexaploids were also discovered in the SmithRiver valley and on the coast of Del Norte County in northwestern California (Figure 4).In these heretofore unreported contactzones as well as throughout the study area, mixed populations, pentaploidhybrids, and disjunct populations were found.Four populations with tetraploid and hexaploid plants growing togetherwere noted in Washington (604, 627) and Oregon (56, 428). As mentionedpreviously, 12 pentaploid plants were found.Undoubtedly many more mixed populations and hybrids exist in nature, butgo undetected because they lack morphological distinctness.Disjunct populations were more common than reported by Lawrence, who mentioned theoccurrence of one tetraploid plant in a hexaploidarea of northwest California.For example, isolated 4x populationswere found in coastal Washington at Grays Harbor and along the coast of California.Raven's (1963) re- port of a tetraploid from San Clemente Island off thesouthern Cali- fornia coast is another apparent disjunct.Similarly hexaploids occurred in predominantly 4xareas, for example, in the Hoh River valley on the Olympic Peninsula,on Vancouver Island, and in the Scott River valley of northern California. 21 Also unexpected was the discovery of septaploid and octoploid plants among hexaploids (399) in a supposedly tetraploid area near Langley, British Columbia. The octoploid reported by Lawrence to occur at Squaw Valley in Fresno County, California was not located, though a pentaploid (332-1) and hexaploids (331) were found there. The penetration of the coastal hexaploids inland in some river valleys in Oregon, and the presence of tetraploids in close proximity to the coast in others are further evidence that the two forms are not segregated ecologically according to coastal or inland climatic regimes.In the Siuslaw, Nestucca, and Columbia River valleys, hexaploids were found 26, 16, and 45 miles inland.In the Yaquina River valley, tetraploids were found eight miles from the river mouth. At the mouth of the Columbia River, large populations of tetraploids (338, 628, 627) were established along the river bluffs. As a general pattern, it appears that the distributions of 4x and 6x Achillea are closely related to the river systems.In Washington, the inland extension of the coastal hexaploids coincided with the Chehalis and Cowlitz River drainages (Figure 2).In northern California (Figure 4), a more striking correlation is observed. Tetraploids were found in the Scott, Salmon, and upper Klamath River canyons, while the hexaploids were found in the Mad, Van Duzen, Trinity and lower Klamath River valleys.The transition between 4x and 6x occurred at the merger of the Klamath and Trinity 22 Rivers.Tetraploid plants of this transition area were producing unreduced spores. A similar situation is observed in southwestern Oregon.Tetraploids were present in the Rogue River valley and hexaploids were in the Umpqua (South Fork) and Illinois valleys. These three river systems are separated by mountains 3500 to 4000 feet high. The complex distributions of tetraploid and hexaploid Achillea described above appear to result from basic patterns, established far in the past, that have been modified by more recent events.It is suggested that much of the complexity has resulted recently by the interaction of three factors--the reproductive system of Achillea, the availability of habitats, and man's involvement in the creation of such habitats. Achillea's genetic, reproductive, and vegetative characteris- tics favor movement of plants throughout a region.High chromosome number, normal pairing, and obligate outcrossing promote genetic variability and thus ecological diversity.The prolonged flowering period, the large number of achenes that a single plant produces, and the simple germination requirements of the fruits contribute to its dispersibility.Establishment and survival of progeny are aided by vigorous vegetative growth and perenniality.Such features are considered by Ehrendorfer (1965) to be those of colonizing plants.Indeed, species of the A. millefolium complex are widely 23 distributed in varying environmental conditions throughout the northern hemisphere. However in western North America, the availability of suitable habitats appears to limit the movement of Achillea.Tetraploids and hexaploids are found in habitats of open sites,i. e. ,forest clearings, bald areas, or river banks and sand bars.In western Oregon, inso- lation was thought to be a principal controlling factor in habitat availability (Tyrl, 1969).It was noted that while vegetative plants were occasionally observed under a forest canopy, flowering plants were present only in the more open areas.Further observations of Achillea in Washington and California confirmed this.The dense coniferous forests of the Coast, Olympic, and southern Klamath Mountains then form a barrier to migration except that by long range dispersal. As is immediately apparent, the river systems provide natural open habitats through the mountains. In addition to its natural habitats, A chillea occurs abundantly in habitats created by man.Lumbering, farming, highway and railroad construction, and conservation practices have created and are creating numerous open habitats.Plants are no longer barred from stepwise migration. Man is apparently also instruMental in dispersal.Railroad and highway construction, especially With the advent of the interstate freeway, frequently involves movement of soil for long distances, and with it, the dispersal of plant seeds and fruits. 24 Indications of this in Achillea are the disjunct tetraploids (60, 261) and hexaploids (358, 512, 607), which in each case were established in the graveled margin of the highway.

Cytogenetic Relationships

The hexaploids studied are considered to be indigenous to North America and arise from tetraploid progenitors by the union of reduced with unreduced gametes. The following observations are cited as evidence for this conclusion:

1.There are strong reproductive barriers between the Old World and New World hexaploids.Following attempts to hybridize the European hexaploid with the North American one, Clausen et al. (1940) reported that no viable progeny were obtained.Subsequent hybridizations (Hiesey & Nobs, 1952; Ehrendorfer, 1952a; Mulligan & Bassett, 1959) resulted in the production of sterile F1 hybrids.In contrast to Hiesey and Nob's interpretation that the barrier is due to a divergence of the genetic systems, this lack of viable progeny probably indicates the absence of any close phylogenetic connection between the two hexaploids.This suggests, therefore, that the North American form is not directly derived from the European form.

2.The marked similarities observed in morphological characters, habitats, and environmental responses between the North 25

American tetraploids and hexaploids indicate autopolyploidy.The morphological and ecological variability of Achillea has been extensively documented (Clausen, Keck & Hiesey, 1940, 1948; Hiesey, 1953; Hiesey & Nobs, 1952; Ehrendorfer, 1952b).In each study, the investigators recognized and commented on the complete intergradation or apparent parallelism between the tetraploids and hexaploids, and on the lack of well defined differentiating characters. Such phenomena are described as being characteristic of autoploid changes in chromosome number (Stebbins, 1950; Davis & Heywood, 1963). This complete morphological and ecological intergradation may be attributed to autoploidy and the continuing relative ease of gene exchange between the tetraploids and hexaploids.Reproductive isolation or diminished gene flow between the different ploidy levels do not appear to be significant in Achillea.In theory, such isolation should result from the sterility of intermediate hybrids (triploids) between diploids and tetraploids.However in Achillea, gene flow is at higher ploidy levels, the chromosomes are apparently homologous, and there is a high degree of pairing, resulting in a fertile pentaploid intermediate.The occurrence of such 5x plants in nature, and their production of 4x and 6x, as well as aneuploid, progeny indicates possible gene flow in both directions. As a further point, the formation of unreduced gametes can result in direct gene transfer from the tetraploid level to the hexaploid level.This has been reported in Achillea 26 (Ehrendorfer, 1959) as well as in other genera, for example, Artemisia (Estes, 1969), Solanum (Marks, 1966), Delphinium (Lewis et al., 1951), and Dactylis (Carroll & Borrill, 1965).Finally, gene flow is not prevented by the lack of a hybrid habitat.The tetraploids, pentaploids, and hexaploids grow in the same ecological niche--open, usually disturbed habitats.Ploidy is not a determinant in habitat success.The ready germination of the achenes produced by 4x, 5x, and 6x plants, combined with perenniality and vigorous growth, facili- tate immediate survival and subsequent gene exchange.These data indicate that the 4x and bx genetic systems are neither isolated nor incompatible.

3.In the Alsea and Columbia River valleys, two tetraploid populations (56, 627) were producing unreduced spores.In each population a single hexaploid plant was found, being morphologically indistinguishable. Adjacent 4x populations did not have such gametes. Similarly the discovery of septaploid and octoploid plants suggests that the production of unreduced spores is the mechanism of increase in chromosome number. These plants were among hexaploids (399), some of which were producing unreduced spores.Tetra- ploids (400) also producing unreduced spores were nearby.It may be that the octoploid plant is the result of the union of 2x and 6x gametes and then backcrossing with surrounding hexaploids to produce a septaploid hybrid. A second possibility is that the septaploid is not a 27 hybrid, but the result of a union of 4x and 3x gametes.In addition, numerous hexaploid populations are found among tetraploid populations producing these spores.Examples are the previously mentioned populations in southwestern Oregon and northwestern Washington.

Cytogeographic Patterns

Relating the distribution of tetraploid and hexaploid Achillea and the cytogenetic data, favoring autoploid origins of the hexaploid, a sequence of events in the development of the present cytogeographic pattern may be hypothesized.I previously speculated (1969) that originally only tetraploid Achillea migrated into North America from Eurasia.Following establishment in the Klamath Mountains, populations to the north were eliminated or reduced. This loss was perhaps associated with Pleistocene climatic changes.The tetraploids, by means of unreduced gametes, then gave rise to hexaploids at the northern coastal limits of the mountains.The hexaploids, possibly having a momentary selective advantage, became established, and dispersed northward along the Oregon Coast.Questions which I raised as to the significance of the tetraploids on the Olympic Peninsula and at the southern limits of the Klamath Mountains are partly clarified by the results of this further study. This pattern of coastally restricted and geographically separated 28 hexaploid distribution, is suggested to be the result of 6x formation in two regions.The Klamath and Olympic Mountains appear to be areas of original tetraploid colonization and subsequent centers of hexaploid formation.The tetraploid distribution in these areas closely coin- cides with independently defined geological boundaries.Tetraploid populations producing unreduced spores are concentrated at the margins of these mountain masses (Figure 10).The occurrence of 6x populations among these tetraploids is an indication of active forma tion of hexaploid plants, their establishment probably being aided by vigorous vegetative growth and perenniality.In general, the hexaploids migrated along the coasts and the tetraploids inland. The restriction of the hexaploids to the coasts of Oregon, Washington, and British Columbia and the tetraploids to the interior may be accounted for by the lack of suitable habitats, a distributional factor stressed earlier.The dense coniferous forests of the Coast Range mountains, in which few natural open sites occur, formed a barrier between the coastal hexaploids and the interior tetraploids. The importance of the river systems in the continual creation of open habitats is apparent.The wide distribution of the hexaploid in central California, a region characterized by extensive open grasslands and oak savannah, supports this suggestion that habitat availability is a principal determinant of distribution.The advent of man and his creation of habitats has facilitated the dispersal of both tetraploids 29 and hexaploids, leading to the dynamic distributional pattern that is now revealed.

Taxonomic Treatment

Achillea in North America is a challenging problem for the taxonomist.In developing a system of species and infraspecific categories, he must resolve in some way the contrasting factors of cytological discontinuity, due to polyploidy, and morphological con- tinuity, due to intergradant ecotypes and geographical races.Achillea in North America was first distinguished taxonomically from the European A. millefolium L. in the early 19th century.Bongard, in 1832, described plants from Sitka, Island as A. borealis and Nuttall, in 1834, described A. lanulosa from specimens collected by Meri- weather Lewis on the Clearwater River of Idaho. Around 1900, a series of additional species names were proposed by Pollard, Ryd- berg, Greene, and others.The genus became a model for the appli- cation of cytological and experimental techniques in systematics, when it was incorporated into the research program at the Carnegie Institution laboratories at Stanford University.There, it was the subject of a series of special studies on the nature of species by Clausen, Keck & Hiesey (1938, 1940, 1948, 1958) and their coworkers.The conclusion drawn by these workers was that there are three distinct ecospecies in North America, two hexaploids and one 30 tetraploid.One hexaploid, A. millefolium L.is an introduction from Europe and was believed to be established throughout the eastern portion of the United States.However, recent studies (Ehrle, 1958; Mulligan & Bassett, 1959) have indicated that this taxon has only a very limited occurrence in North America and that most of the East Coast populations of Achillea are tetraploid.The second hexaploid is A. borealis Bong., found along the Pacific Coast from Alaska to Baja California, and composed of three ecotypic subspecies.The tetraploid ecospecies, A. lanulosa Nutt. , was described as being highly polymorphic but divisible in the Pacific Coast states into two ecotypic subspecies. Such a classification is the result of emphasis by Clausen et al. on reproductive isolation, the limits to an ecospecies being defined in terms of free gene exchange without loss of fertility. A polyploid difference in chromosome number was thought to create a nearly complete barrier to this exchange.Thus to many workers, chromosome number becomes a convenient means of delimiting ecospecies in a polyploid complex. Achlys (Fukuda, 1967) and Kohlrauschia (Ball & Heywood, 1962) illustrate the formal taxonomic recognition of various ploidy levels when the difference in chromosome number is associ= ated with differences, however slight, in morphology, ecology, and geography. A more restrictive view is advanced by LOve (1951, 1960, 1962, 1964), who contends that cytological features that reduce or 31 prevent gene exchange should always take precedence over morphological differences, so that different ploidy levels should be classified as separate species. Chromosome number differences would be applied even when there are no morphological or ecological characters that allow taxonomists consistently to distinguish between plants at different ploidy levels.Examples of such an approach are in the genera Hierochloe (LOve & LOve, 1965) and Anthoxanthum (Liive & LOve, 1948, 1961; cf, Hedberg's 1961 study). The results of this study raise questions as to the validity of this assumption that a change in chromosome number prevents gene exchange. The discovery of fertile pentaploid hybrids that yield both 4x and 6x progeny, of mixed populations containing 6x plants among 4x plants that form unreduced garrietes, and of plants representing still higher polyploid levels suggest that Achillea in western North America should be characterized as one genetic system composed of two or more ploidy levels, rather than two distinct systems separated by genetic barriers.The use of the single criterion of chromosome number to define species obscures this basic biological relationship. Therefore, it is here suggested that the two ploidy levels of Achillea do not require taxonomic recognition, and that a single species en- compassing tetraploids and hexaploids adequately and meaningfully describes the genus in western North America. At the infraspecific level, attempts have been made to relate 32 ploidy level with morphology, ecology, and geography. Nobs (1960) proposed that there is one species, A. millefolium L. ,extremely polymorphic but with recognizable ecotypes which may be designated as varieties. On morphological characters and chromosome number, he described ten varieties, which were considered to be generally distinct but with intergrading intermediates.Thus the species and subspecies of Clausen, Keck & Hiesey were reduced to varietal rank. However, there are two problems inherent in such a classification.First, as pointed out above, the two ploidy levels are merely two phases of one genetic system and are not clearly sorted geographically or ecologically.Second, because there are no consistent differences in morphology between the 4x and 6x and there is con- siderable inter- and intra-population variation, recognition of varieties in the field is impossible or at best tenuous. As Clausen, Keck & Hiesey (1948, p.1) state:11... the series of variations within the complex have no morphological breaks but consist of trends that seem to be indissolubly linked together by finely graded morphological steps." Furthermore, there appears to be no single factor which can be used consistently to separate tetraploids and hexaploids.Lawrence (1947) noted that chromosome number provided the only dependable means of distinguishing 4x from 6x plants, as morphological characters were inadequate.Ehrendorfer (1952b) confirmed this, but reported that pollen grain size was a definite character for the 33 separation of tetraploids and hexaploids as herbarium specimens. This approach was employed by Mulligan & Bassett (1959) for distributional studies in North America and by Oswiecimska & Gawlowska (1967) for Europe. However, Figure 11 shows the variability in grain size in a sample of plants from my chromosome survey.Calculation of the coefficient of determination revealed a value of 0.533, indicat- ing that only 53. 3% of the variation in pollen grain size can be accounted for by the difference in ploidy level. A similar result was reported by Raven et al. (1968), who found that pollen diameters of Ambrosia dumosa could be used to separate diploids from polyploids but that separation of tetraploids and hexaploids was difficult.Other examples of ambiguous separations may be found in the literature, and Davis & Heywood (1963) question the use of such indicators of ploidy. Achillea has been one of the classic examples of ecotypic evolution, with Clausen, Keck & Hiesey (1948) dividing the genus into geographically distinct tetraploid and hexaploid ecospecies, each with parallel ecotypes.This concept (Clausen, Keck & Hiesey, 1939; Clausen & Hiesey, 1958;. Clausen, 1967) emphasized the physiological adaptions of populations to the environment and the free exchange of genes between populations.Differences in chromosome number within or between populations, therefore, were assumed to reduce gene flow. However, it appears from the evidence already discussed that 34

53-2 121-2

70-1

68-1

Tetraploids 51-3 (2n=36) 104-1

92-2 --ESE111113-- 54-1

127-3

83-3

59-1

84-1

78-1

64-1

Hexaploids 135-3 (2n=54) 108-2

113-1

98-1

61-2

73-1

Septaploid 399-9 (2n=63)

Octoploid 399-10 (2n=72)

1 F

16I I 21 181 19 20 21 22 23 24 25 26 27 28 29 311 32

Figure 11.Comparison of internal pollen grain diameters of Achillea. Range, mean, standard deviation, and standard error of ten grains.Sizes in microns. 35

Achillea requires an expansion of the ecotype definition developed by Clausen and coworkers to include plants of different ploidy levels capable of gene exchange.The rate of gene flow is not a crucial factor here, and plants at both levels are capable of contributing con- tinuously to the gene pool.If one does not expand the concept, the tetraploids and hexaploids must be designated two different ecotypes. Such an approach is arbitrary, focusing only on chromosome number and ignoring all other factors.The biological situation is not being fully described.In contrast, defining ecotypes in terms of climatic and edaphic conditions, and the morphological and physiological responses of different plant species to them, as proposed by13Ocher

(1967, p. 257),is readily applicable to Achillea. There are precedents for this recommendation that subspecific taxa not be recognized in Achillea.Epilobium latifolium (Small,

1968)and Ambrosia dumosa (Raven, et al.,1968)are species similar to Achillea in that different ploidy levels, morphologically insepar- able, were reported but not formally described. It must be noted that Achillea from northern Alaska, the Arctic regions, and the East Coast was not extensively examined.Until this has been done and the connections between North American and Eurasian material thoroughly investigated,it is suggested that a detailed revision of the taxonomy not be undertaken.. On the basis of present information, it is felt that the yarrows of western North 36 America can be described as a single species, highly polymorphic and with two or more ploidy levels. 37

BIBLIOGRAPHY

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Ball, P. W. and V. H. Heywood.1962.The taxonomic separation of the cytological races of Kohlrauschia prolifera (L.) Kunth sensu lat. Watsonia 5: 113 -116. liso.cher, T. W.1967.Continuous variation and taxonomy. Taxon 16: 255-258.

Campbell, C. D.1953.Washington geology and resources. Washington State University Research Studies 21: 114 -153.

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Clausen, J. and W. M. Hiesey.1958.Experimental studies on the nature of species.IV.Genetic structure of ecological races. Washington, D. C.312p.(Carnegie Institution of Washington. Publication 615) Clausen, J., D. Keck and W. M. Hiesey.1938. Chromosome number and environment.In:37th yearbook of the Carnegie Insti- tution of Washington.Washington, D. C.p. 220-221.

1939.The concept of species based on experiment. American Journal of Botany 26:103-106.

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Clausen, J., D. Keck and W. M. Hiesey.1948.Experimental studies on the nature of species.III.Environmental responses of climatic races of Achillea.Washington, D. C.129p. (Carnegie Institution of Washington.Publication 581)

Davis, P. H. and V. H. Heywood.1963.Principles of angiosperm taxonomy.Princeton, D. Van Nostrand.556p.

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1952b.Cytotaxonomic studies of Achillea.In: 51st yearbook of the Carnegie Institution of Washington. Washington, D. C.p. 125-131.

1959a.Differentiation-hybridization cycles and polyploidy in Achillea.Cold Spring Harbor Symposia on Quantitative Biology 24: 141 -152.

1959b.Spindeldefekte, Mangelhafte Zwellwandbil- dung und andere Meiosest5rungen bei polyploiden Sippen des Achillea millefolium Komplexes.(Zur Phylogenie der Gattung Achillea, III.) Chromosoma (Berlin) 10: 461-481. 1960. Akzessorische Chromosomen bie Achillea: Auswirkungen auf das Fortpflanzungssystem, Zahlenbalance und bedeutung Kir die Mikro-Evolution.(Zur Phylogenie der Gattung Achillea, VI.) Zeitschrift fur Vererbungslehre (Berlin) 91: 400-422.

1965.Dispersal mechanisms, genetic systems, and colonizing abilities in some flowering plant families.In: The genetics of colonizing species, ed. by H. G. Baker and G. L. Stebbins. New York, Academic.p. 331-352. Ehrle, E. B.1958. A cytotaxonomic study of the genus Achillea in Pennsylvania. Rhodora 60: 7 -9.

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Hiesey, W. M.1953.Comparative growth between and within climatic races of Achillea under controlled conditions.Evolution 7: 297-316.

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LOIve, A. and D. LOve.1965.Taxonomic remarks on some Ameri- can alpine plants.Boulder.43p.(University of Colorado Studies.Series in Biology no. 17)

Marks, G. E.1966.The origin and significance of intraspecific polyploidy:experimental evidence from Solanum chacoense. Evolution 20: 552-557. Mulligan, G. A. and I. J. Bassett. Achillea millefolium complex in Canada and portions of the United States.Canadian Journal of Botany 37: 73-79.

Nobs, M.1960.Achillea.In:Illustrated flora of the Pacific States, ed. by R. S. Ferris.Vol. 4.Stanford University. p. 390-391.

Oswiecimska, M. and M. Gawlowska.1967.Pomocnicze metody oznaczania taksonow swiorowego gatunku Achillea millefolium L. s.1.I.Korelacja miedzy floidalnoscia, ploidalnoscia, wielkoscia ziarn pylku i chemizmen rosliny.Herba Polonica le: 3 -11.(Abstracted in Biological Abstracts 49: no. 30983. 1968) Raven, P.1963. A flora of San Clemente Island, California.Aliso 5: 289-347.

Raven, P.,D. W. Kyhos, D. C. Breedlove and W. W. Payne.1968. Polyploidy in Ambrosia dumosa (Compositae: Ambrosieae). Brittonia 20:205-211.

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Snow, R.1963.Alcoholic hydrochloric acid-carmine as a stain for chromosomes in squash preparations.Stain Technology 38: 9- 13. 41

Stebbins, G. L.1950.Variation and evolution in plants. New York, Columbia University.643p.

Taylor, R. L. and G. A. Mulligan.1968.Flora of the Queen Charlotte Islands.Part II.Cytological aspects of the vascular plants.Ottawa.148p.(Canada. Department of Agriculture. Research Branch. Monograph no. 4) Turesson, G.1939. North American types of Achillea millefolium L.Botaniska Notiser (Lund), 1939, p. 813-816.

Tyrl, R. J.1967.Cytogeography of Achillea millefolium L. in western Oregon. Master's thesis.Corvallis, Oregon State University.79 numb. leaves.

1969.Cytogeography of Achillea millefolium in western Oregon.Brittonia 21:215-223. APPENDIX Appendix I.Collection data and chromosome number determinations forAchil lea accessions,

AccessionChromosome Number of numbernumber (n) counted State County Range Township Section Notes

45 18 1 Colorado Jackson 82W SN 15 East end of small lake along U. S. Hwy. 40, ;0.5 mi. W of Routt Nat.. For, boundary marker.

46 18 1 Colorado Eagle 80W 5S 13 Scattered plants just inside entrance to Gore Creek Campground. Arapahao Nat. For.

47 18 1 Colorado Gunnison 81W 14S Taylor Lake Rd., 0.5 mi. S of Cotton- wood Pass. Above timberline, Elev. 12,126 ft.Large population.

48 18 2 Colorado Gunnison lE 51N 27 Small population in Almont Camp- ground, Gunnison Nat. For. , 300 ft. N of outdoor facilities.

49 18 1 Colorado Gunnison 7W 49N 6 Road into Gunnison R. gorge, 0. 5 mi. E of junction with Black Canyon National Monument Rd.Large population.

136 18 1 California Siskiyou SW 17N 16 Start of Twin Valley Trail, E-side of Young's Valley, elev. ca. 4000 ft. R. L. Carr.

157 18 3 Alaska Rich Hwy.; Delta R. ; Black Rapids Glacier.A. Legge.

158 18 1 Alaska Lower Camp; Eagle Creek; Eagle Summit. A. Legge.

159 1 Alaska Vicinity of Morine Trail near McKin- ley Park Hotel, McKinley Park.A. Legge. 04, t.) Appendix I Continued. AccessionChromosome Number of number number (n) plants counted State County Range Township Section Notes 160 18 2 Alaska Mastedon Fork of Eagle Creek, Eagle Summit. A. Legge.

161 18 1 Alaska Mileage Marker 55, Deneli Hwy. "only one seen for miles". A. Legge.

162 18 1 Alaska Richardson Hwy. A. Legge.

163 18 1 Alaska -- Delta River; Richardson Hwy. ; Donnelly; Alaska Range. A. Legge. 164 18 3 Alaska Eagle Creek at 2700 ft. elevation, Eagle Summit. A. Legge.

165 18 1 Alaska Mammoth Creek; Eagle Summit; Steese Hwy. A. Legge.

166 18 1 Alaska Mammoth Creek; Steese Hwy. at (36) mileage marker 117, 104 mi. N of Fairbanks.A. Legge 8.

167 18 1 Alaska At junction of Mastedon and Miller Creeks; Eagle Summit; Steese Hwy. ca. 105 mi. N of Fairbanks.A. Legge 1.

168 18 1 Oregon Harney 33E 32S 24 Achenes; six plants ca. 7 nil. W of Fish Lake on Loop Rd.Elev. 6000 ft. Steens Mountains. W. L. Ezell.

169 18 1 Oregon Harney 34E 32S 31 Achenes, seven plants on rim of Kiger Gorge; Steens Mountains, Elev.. 8500 ft.W. L. Ezell

170 18 1 Oregon Harney 31E 23S 2 Achenes, four plants ca. 5 mi. E of Burns, on U. S. Hwy. 20. W. L. Ezell. Appendix I Continued. AccessionChromosome Number of number number (n) plants counted State County Range Township Section Notes

171 18 1 Oregon Harney 25E 24S 21 Achenes from one plant on Squaw Butte.W. L. Ezell.

172 18 1 WashingtonYakima 11E 8N 8 Achenes, two plants on S-slope of ridge between Mazama and Klickitat Glaciers.Elev. 6500 ft.A. Legge.

173 18 1 California San Bernardino lE 2N 27 Small population opposite Moonridge Club House and San Bernardino Nat. For, boundary marker 2N05. Big Bear Lake. 174 18 2 Alaska Mammoth Creek; Steese Hwy.- A. Legge.

180 18 2 California Siskiyou 6W 46N 18 Base of steep 5-facing roadside bank 0.7 mi. N of Klamath R. turnoff on U. S. Hwy. 99,

181 18 2 California Siskiyou 5W 41N 11 Roadside fill bank on U.S. Interstate 5, 10.0 mi. S of Weed city limits. 182 18 2 California Shasta 5W 36N 34 Small population, U. S. Interstate 5 right of way, 300 ft. W of Vollmer Chevron station, 2. 5 mi. N of Dog Cr. bridge. 183 18 2 California Nevada 7E 16N 24 Roadside bank on Calif. Hwy. 20, 0. 9 mi. W of Rough & Ready city limit sign. 184 18 2 California Nevada 8E 15N 11 Roadside bank, 150 ft. N of 2000 ft. elev. marker, 4. 4 mi. S of Grass Val- ley city limits on Calif. Hwy. 49. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

185 18 2 California Placer 8E 13N 5 Small population, between Calif. Hwy. 49 and fence row, 2.1 mi. S of Placer Co. line.

186 18 2 California El Dorado 8E 12N 12 E-facing roadside bank of Calif. Hwy. 49, 2.0 mi. S of El Dorado Co. line.

187 18 2 California El Dorado 9E 11N 6 Large population on steep E-facing bank 300 ft. S of Pilot City city limits on Calif. Hwy. 49. 188 18 2 California El Dorado 10E 11N 18 Culvert ditch under Calif. Hwy. 49, 2.0 mi. N of bridge over S. Fork of American R. 189 27 2 California El Dorado 10E 11N 17 Small population in Calif. Hwy. right of way 0.1 mi. S of Coloma city limitsign within the city. 190 18 2 California El Dorado 10E 11N 35 Roadside bank at turnout on ridge route of Calif. Hwy. 49, 4.9 mi. S of Colloma city limits. 191 18 2 California El Dorado 11E lON 18 E-facing bank on Calif. Hwy 49, 0. 5 mi. S of Placerville city limit sign.

192 18 3 California San Bernardino 1E 2N 22 Large population at edge of golf course at intersection of Moonridge Rd. and Sunset Rd. Big Bear Lake.

193 27 2 California El Dorado 10E 8N 14 Fence row W of Calif. Hwy. 49, 1. 1 mi. N of Amador Co. line. 194 27 2 California Amador 10E 7N 22 Grassy hillside adjacent to Calif. Hw5s 49, 0. 4 mi. S of junction with Calif. Hwy. 16, Appendix I Continued. Accession ChromosomeNumber of number number (n) plants counted State County Range Township Section Notes

195 27 2 California Amador 11E 6N 21 Grassy E-facing bank on Calif. Hwy. 49, 0.7 mi. S of junction with Calif. Hwy. 88.

196 27 2 California Amador 11E 5N 2 Grassy roadside cut on Calif. Hwy. 49, 0. 4 mi. N of Calaveras Co. line and North Fork of Mokelumne River bridge.

197 27 2 California Fresno 21E 14S 3 Bank above irrigation canal passing under Calif. Hwy. 180, 300 ft. W of junction with Highland Rd.

198 27 2 California Fresno 23E 14S 7 Irrigation canal bank, 50 ft. N of Calif. Hwy. 180, O. 8 mi. W of Centerville city limit sign. 199 27 2 California Orange 7W 7S 33 Steep roadside bank 3. 9 mi. E of junction with U. S. Hwy. 101 on Calif. Hwy. 74,

200 27 2 California Orange 6W 7S 19 Calif. Hwy. 74, 9.2 mi. E of junction with U. S. Hwy. 101, steep bank.

201 18 1, 1 California San Diego 4E 14S 16 Large population in Cuyamaca Rancho 2n = 45 State Park in campground area.

202 18 1 California San Bernardino lE 2N 19 Vacant lot, waste area at corner of Gay and Lakeview streets, Big Bear.

203 27 2 California San Luis Obispo 12E 30S 18 Grassy roadside bank on Calif. Hwy. 1, 3. 9 mi. S of entrance to Camp San Luis Obispo. Appendix I Continued. Accession ChromosomeNumber of number number (n) plants counted State County Range Township Section Notes

204 27 1 California San Luis Obispo 10E 29S 25 Sand dune beach area 300 ft. from high tide line, W of Sandlewood and Azure Street intersection, Morro Bay.

205 27 2 California San Luis Obispo 7E 26S 27 Roadside fill bank over deep gully, 0. 5 mi. N of San Simeon Nat. Monu- ment on Calif. Hwy. 1. 206 27 2 California San Luis Obispo 6E 26S 19 Exposed coastal bluffs on small point along Calif. Hwy. 1, 4.3 mi. S of Piedras Blancas Lighthouse Rd. junction. 207 27 2 California San Luis Obispo 6E 26S 14 Exposed roadside of Calif. Hwy. 1, 1.0 mi. N of Piedras Blancas Light- house Rd. junction.

208 27 2 California San Luis Obispo 6E 25S 9 Exposed W-facing coastal bluff.ca. 500 ft. above water on Calif. Hwy. 1, 1.0 mi. S of Monterey Co. line. With Stachys and Castellija.

209 27 2 California Monterey SE 24S 8 W-facing bank above Calif. Hwy. 1, 0. 9 mi. S of Gorda city limit sign.

210 27 2 California Monterey SE 23S 18 Steep roadside cut on Calif. Hwy. 1, 6.7 mi. N of Gorda city limit sign. 211 27 2 California Monterey 4E 22S 27 Coastal bluff 3.6 mi. N of Los Padres Nat. For, boundary marker near Lucia on Calif. Hwy. 1 and 2. 3 mi. S of Vincinte Cr. bridge.

212 27 2 California Monterey 3E 21S 22 Coastal bluffs 3. 6 mi. S of Hot Springs Cr. bridge on Calif. Hwy. 1. Appendix I Continued. Accession ChromosomeNumber of number number (n) plants counted State County Range Township Section Notes

213 27 2 California Monterey 3E 20S 30 Base of steep coastal bluff 5.0 mi. N of Hot Springs Cr. bridge on Calif. Hwy, 1.

214 27 1 California Monterey 2E 19S 30 E-facing roadside bluff at edge of dense forest directly opposite Big Sur city limit sign. 215 27 2 California Monterey 1E 18S 32 Coastal bluff on Calif. Hwy. 1, 9, 1 mi. N of Big Sur city limit sign. 216 27 2 California Monterey 1W 17S 25 Large population, roadside of Calif. Hwy. 1, 0.6 mi. N of Grappata Cr. bridge and 7. 9 mi. S of Point Lobos State Reserve. 217 27 2 California Santa Cruz 1E 12S 10 Population on lee side of stabilized dunes opposite campground area in Sunset Beach State Park. 218 27 2 California Santa Cruz 3W 11S 15 Coastal bank on Calif. Hwy. 1, 4. 8 mi. S of Bonny Doon-Felton turnoff, with Raphnus and Bromus. 219 27 2 California Santa Cruz 3W 10S 33 Coastal bluffs on Calif. Hwy. 1, 2.2 mi. N of Bonny Doon-Felton turnoff. 220 27 2 California San Mateo SW 9S 14 Roadside bank on Calif. Hwy, 1, 3. 3 mi. N of San Mateo-Santa Cruz Co. line.

221 27 2 California San Mateo SW 8S 8 Roadside cut in coastal bluff, 1. 3 mi. S of San Mateo County Park turnoff on Calif. Hwy. 1. Appendix I Continued. Accession ChromosomeNumber of number number (n) plants counted State County Range Township Section Notes

222 27 1 California San Mateo 5W 6S 5 1. 2 mi. N of Tunitas Creek bridge on Calif. Hwy. 1. 223 27 2 California San Mateo 6W 5S 15 Irrigation ditch, E side of Calif. Hwy. 1, 0.5 mi. S of Half Moon Bay airport. 224 27 2 California San Mateo 6W 4S 10 Drainage ditch one block west of Linda Mar Ave. and intersection with Calif. Hwy. 1, Pacifica, Calif. 225 27 2 California San Mateo 6W 3S 25 Steep roadside cut on Calif. Hwy. 35 (Skyline Dr.) 0.5 mi. N of intersection with Sneath Lane and 4. 9 mi. N of Burlingame city limits. 226 27 2 California San Mateo 5W 4S 15 Roadside bank on Calif. Hwy. 35 (Skyline Dr. ) 0. 4 mi. S of Burlingame city limit sign.

227 27 2 California San Mateo 5W 5S 1 Large population on banks above Calif. Hwy. 35 (Skyline Dr.) 0.2 mi. S of junction with Bunker Hill Rd. Area soon to be obliterated by freeway. 228 27 2 California San Mateo 4W 5S 17 Steep roadside cut on Canada Rd. 0. 8 mi. S of junction with Ralston Rd. 229 18 2 California San Mateo 4W 5S 27 Open area among Quercus, 0.1 mi. E of Canada Rd. intersection on Edge- wood Rd.

230 27 2 California San Mateo 4W 5S 23 Untended fence row, northside of Edge- wood 511: E bf Cadada Rd.. 41, intersection Suburbs of gedvd-ood City. Appendix I Continued. Accession ChromosomeNumber of number number (n) plants counted State County Range Township Section Notes

231 27 2 California Alameda 1W 4S 10 Base of steep hills, 0.2 mi. E of Union City city limits on Calif. Hwy. 84 and 0.2 mi. E of railroad underpass.

232 27 2 California Alameda 1W 3S 21 Between fence and roadside cut, southside of Calif. Hwy. 84, 1.4 mi. W of Arroyo del Valle and 5. 5 mi. W of Livermore city limits.

233 27 2 California Alameda 2E 2S 26 East-facing roadside bank along untended fence row 3.0 mi. N of junction with U.S. Hwy. 50 on Vasco Rd.

234 27 1 California Solano lE 4N ...- Isolated small population, roadside bank, eastside of Calif. Hwy. 12, 1.0 mi. S of Denverton turnoff.Just N of railroad museum and overpass.

235 27 2 California Napa 3W 4N 8 West facing grassy bank along fencerow on Calif. Hwy. 12, 2.3 mi. N of junction with Calif. Hwy. 29.

236 27 2 California Napa. SW 5N 26 Large population, roadside cut on Calif. Hwy. 12, 0.2 mi. E of Sonoma-Napa Co. line.

237 27 2 California Sonoma 5W 4N 8 Grassy roadside bank on Calif. Hwy. 121, 3.0 mi. S of junction with Calif. Hwy. 12.

238 27 2 California Sonoma SW 4N 30 Steep east-facing roadside cut 0. 2 mi. N of Calif. Hwy. 37 junction on Calif. Hwy. 121. Bank above u- marshlands; ca. 20 ft. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County RangeTownship Section Notes

239 27 1 California Mark 6W 3N 12 Population on grassy bank of freeway frontage road (Calif. Hwy 37) 0.6 mi. W of Marin- Sonoma Co. line.Edge of woods. 240 27 2 California Mark 6W 1N 33 Roadside bank, Calif. Hwy. 1, 400 ft. S of Tamalpais Rd. junction, 2.1 mi. N of junction with U. S. Hwy. 101.

241 27 2 California Mark 6W 1N 32 Open hillside 4.0 mi. W of junction with U. S. Hwy. 101 on Calif. Hwy. 1.

242 27 2 California Mark 7W 15 1 Open hillside just above ocean on leeward side of bluffs on Calif. Hwy. 1, 6.7 mi. W of junction with U.S. Hwy. 101.

243. 27 2 California Mark 7W 1N 30 Coastal cliffs ca. 800 ft. above ocean opposite large turnout area on Calif. Hwy. 1, 11.7 mi. W of U. S. Hwy: 101 junction.

244 18 2 California Mark 8W 2N 7 Open valley adjacent to Calif Hwy. 1, behind coastal hills, 2.7 mi. S of Olema city limit sign.

245 27 2 California Mark 9W 3N 8 Roadside ditch on Calif. Hwy. 1 above Tomales Bay 6.1 mi. N of Olema city limit sign.With Stachys. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

246 18 2 California Marin lOW 4N 13 Roadside of Calif. Hwy. 1 just above Tomales Bay beach a 0 mi. N of Marshall City city limit sign. 247 18 2 California Marin 9W SN 6 Open farmland, along fence row 4. L mi. N of Tomales city limit on Calif. Hwy. 1. 248 27 2 California Sonoma 11W 6N 27 Roadside cut on Calif. Hwy. 1, 0. 5 mi. S of Bodega Bay city limits. 249 27 2 California Sonoma 12W 7N 14 Coastal bluffs on Calif. Hwy. 1, 2. 5 mi. N of junction with Calif. Hwy. 116.

250 27 1 California Sonoma 13W 8N 26 Roadside waste area on coastal cliffs 0.7 mi. N of Fort Ross State Park on Calif. Hwy. 1. 251 27 2 California Sonoma 14W 9N 10 0. 4 mi. S of Stewarts Point city limits on Calif. Hwy. 1; coastal cliffs. 252 27 2 California Mendocino 15W 11N 33 Roadside bank opposite intersection of Calif. Hwy. 1 and Ocean Drive, Gualala, Calif. 253 27 2 California Mendocino 16W 12N 33 Calif. Hwy. 1 roadside at Iverson Rd. intersection, 8. 8 mi. S of Garcia R. bridge.

254 27 2 California Mendocino 4E 14N 12 Steep S-facing bank on Calif. Hwy. 1, 2. 9 mi. S of Glenwood Creek bridge. u-i tv Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

255 18 2 California Mendocino 17W 16N 33 Coastal cliff O. 8 mi. N of Calif. Hwy. 128 junction on Calif. Hwy. 1. 256 27 2 California Mendocino 4E 19N 30 Fence row on Calif. Hwy. 1, 3. 7 nit, N of Fort Bragg city limits. Open farm land. 257 27 2 California Mendocino 4E 20N 6 Coastal bluff highway cut on Calif. Hwy. 1, 2. 3 mi. S of Westport city limits. 258 27 2 California Mendocino 4E 23N 30 Grassy bank at edge of dense coniferous forest on Calif. Hwy. 1, 0.3 mi. N of Hardy Creek bridge; 21. 5 mi. S of U. S. Hwy. 101 junction.

259 27 4 California Mendocino 4E 24N 6 Large population in grassy open valley, E-side of U. S. Hwy. 101, 0. 9 mi. S of Piercy city limit sign. 260 27 6 California Humboldt 2E 1S 34 W-facing roadside cut on U. S. Hwy. 101, 1. 1 mi. S of the Avenue of the Giants turnoff. Open forest.

261 18 5 California Humboldt 2E 1S 9 U. S. Hwy. 101, edge of forest on banks above Eel R. 3.0 mi. S of Red- crest exit area opened by highway construction. 262 27, 3, 1 California Humboldt 1E 2N 19 Roadside cut on sharp curve of Calif. 2n = 45 Hwy. 36, 0.3 mi. W of Hydesville and 2.3 mi. E of U. S. Hwy. 101 intersection.Partially opened forest. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County RangeTownship Section Notes

263 27 3 California Humboldt 1E 2N 22 Drainage ditch between RR track and Calif. Hwy. 36, 0.2 mi. W of Car- lotta city limits. W-end of bridge over Van Duzen R.

264 27 4 California Humboldt 2E 1N 6 Roadside gravel of Calif. Hwy. 36 in open glade of dense redwood forest, 5.0 mi. W of Grizzly Creek campground entrance.

265 27 3 California Humboldt 3E IN 7 Open valley, roadside waste area N of Calif. Hwy. 36, 2. 9 mi. E of Grizzly Creek campground entrance.

266 18 3 California Humboldt 3E iN 8 East-facing bank on Calif. Hwy. 36, 5.1 mi. E of Grizzly Creek campground entrance. 267 27 3 California Humboldt 3E 1N 17 2. 3 mi. W of Bridgeville city limits. Roadside cut on Calif. Hwy. 36.

268 27 1 California Humboldt SE iN 7 North-facing open hillside on Calif. Hwy. 36, 0.3 mi. E of bridge over S Fork of Van Duzen R.

269 27 2 California Humboldt SE 1N 7 Open grassy hillside W of Calif. Hwy. 36, 2.6 mi. W of Dinsmore city limits.

270 27 1 California Humboldt SE IN 11 South-facing roadside bank at edge of oak woods 2.3 mi. E of Six Rivers Nat. For. boundary marker on Calif. Hwy. 36. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County RangeTownship Section Notes

271 27 2 California Trinity 6E IN 18 Large population among scattered oak trees, grassy banks of Calif. Hwy. 36, 0.3 mi. E of Trinity-Humboldt Co. line. 272 27 2 California Trinity 6E IN 27 Roadside banks at junction of Calif. Hwy. 36 and Ruth Dam Rd. Open grasslands.

273 27 2 California Trinity 8E 15 11 Rocky roadside cut on Calif. Hwy. 36, 0.7 mi. W of North Rattlesnake Creek bridge.

274 27 1 California Trinity 12W 30N 29 3. 1 mi. E of North Rattlesnake Creek bridge on Calif. Hwy. 36.5. 8 mi. W of junction with Calif. Hwy. 3. Open woods. 275 27 4 California Shasta 9W 29N 18 Calif. Hwy. 36 bank, 0.8 mi. E of Trinity Nat. For, boundary marker. 276 27 2 California Shasta 8W 29N 5 Open grassland agrea on Calif. Hwy. A16, 4. 5 mi. N of junction with Calif. Hwy. 36.Elev. 3000 ft.

277 27 1 California Shasta 7W 30N 19 7.7 nil. N of Calif. Hwy. 36 junction on Calif. Hwy. A16. Open grassland.Elev. 3000 ft.

278 27 1 California Shasta 6W 31N 27 Open oak forest on Calif. Hwy. A16, 0. 8 mi. W of junction with Igo-Gas Point Rd.Roadside bank, east side of road. Appendix I Continued, Accession Chromosome Number of number number (n) planb counted State County Range Township Section Notes

279 27 1 California Shasta SW 32N 27 Bank northside of Calif. Hwy. 299, 1.0 mi. E of Keswick turnoff.Fill embankment.

280 27 1 California Shasta 7W 33N 31 South-facing bank under Quercus & Pinus at 2000 ft. elev. marker on Calif. Hwy. 299, 4.3 mi. E of Trinity.. Shasta Co. line. 281 27 2 California Trinity 8W 32N 4 Isolated clump in clearing E of Calif. Hwy. 299, 2.4 mi. W of Trinity- Shasta Co. line.

282 27 1 California Trinity 9W 33N 31 South-facing rocky cut on Calif. Hwy, 299, 2.2 mi. E of Weaverville city limit sign.

283 27 1 California Trinity IOW 33N 8 Roadside bank 1.1 mi. E of Junction City city limit sign on N side of Calif. Hwy. 299.

284 27 1 California Trinity 12W 33N 6 Steep river bank in gorge of N Fork of Trinity R. below Calif. Hwy. 299, 3. 9 mi. E of Del Loma city limits.

285 27 3 California Trinity 8E 5N 34 Open sand-gravel bar of Trinity R. adj. to Calif. Hwy. 299, 0.4 mi. E of Big-French Creek Rd. Large population.

286 27 2 California Trinity 7E 5N 19 South-facing bank of Calif. Hwy. 299, 0.1 mi. E of Don Juan Creek bridge and 3.5 mi. E of Burnt Ranch. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

287 27 2 California Trinity 6E 6N 18 2.9 mi. E of Salyer city limits on Calif. Hwy. 299 on steep north-facing bank above Trinity R. opp, open meadow.

288 18 1 California Humboldt 3E 6N 12 North-facing roadside bank 0.2 mi. S of atezan. Hill turnoff on Calif. Hwy. 299, 289 27 2 California Humboldt 2E 6N 12 Large population, south-facing open hillside on Calif. Hwy. 299, 3.0 mi. E of Kobel turnoff.

290 27 1 California Humboldt lE 7N 5 Small population at edge of North off-ramp to Clam Beach area on U. S. Hwy. 101.

291 27 1 California Humboldt lE 7N 6 Coastal bluff 0.2 mi. N of junction with U. S. Hwy. 101 on Brookhaven Drive towards Luffenholtz Beach, 292 18 1 California Humboldt 1E 8N 31 Coastal bluff on Brookhaven Drive, 1. 5 mi. N of Luffenholtz Beach Park entrance.

293 27 1 California Humboldt lE 9N 17 Graveled margin of U. S. Hwy. 101, opposite Big Lagoon marshland, 5.3 mi. N of entrance to Patrickis Point State Park. 294 27 2 California Humboldt 1E 11N 32 Graveled margin of U. S. Hwy. 101, 300 ft. N of Prairie Cr. bridge, 2. 4 mi. N of Orick. Appendix I Continued, Accession Chromosome Number of number number (n) plants counted State County RangeTownship Section. Notes

295 27 2 California Del Norte 1W 16N 29 Large population, East side of U. S. Hwy. 101, 30 ft. S of Sandmine Rd. intersection, 1.4 mi. S of Crescent City city limit sign. 296 18 3 California Del Norte lE 16N 9 1. 1 mi. W of Six Rivers Nat. For. boundary marker on U. S. Hwy. 199. Open valley at edge of dense redwood forest.. 297 27 2 California Del Norte IE 17N 26 Steep banks of Smith River canyon, 4, 4 mi. N of Six Rivers Nat. For. boundary on U. S. Hwy. 199 and 0.2 mi. E of Hardscrabble Cr. 298 27 1 California Del Norte 2E 17N 23 Smith River valley along fence row, 1. 7 mi. N of Gasquet city limits on U. S. Hwy, 199. 299 18 2 California Del Norte 3E 17N 17 Small population on rocky bank of Smith R. 0.6 mi. N of Howard Griffith Memorial bridge on U.S. Hwy. 199.

300 27 1 California Del Norte 4E 17N 5 Roadside cut of U. S. Hwy. 199, 7.3 mi. W of Siskiyou Nat. For. boundary marker, opposite Bar 0 Ranch mailbox. 301 27 3 Oregon Josephine 9W 41S 2 Large population along U. S. Hwy. 199 opposite Pacific Power substation 2.0 mi. N of Oregon-California state line. Appendix I Continued. Accession Chromosome Number of number number (n) plargs counted State County Range TownshipSection Notes

302 27 2 Oregon Josephine 9W 40S 25 Fence row, East side of U. S. Hwy. 199, 0.5 mi. S of West Fork of Illinois R. bridge. Large population, open pasture land. 303 27 2 Oregon Josephine 8W 39S 4 Open graveled waste area 3.1 mi. N of Cave Junction, Ore. at intersection of Kerby Highline and U.S. Hwy, 199,

304 27 1 Oregon Josephine 8W 37S 24 Single plant along East side of U. S. Hwy. 199 in open conifer-deciduous forest 0.7 mi. S of Hayes Hill Summit.

305 18 1 Oregon Josephine 7W 35S 35 Graveled margin of Robertson Bridge- Wilderville Rd,0. 9 nn. W of bridge.Bluffs of Rogue River valley. 306 18 3 Oregon Josephine 7W 35S 25 Bank opposite public boat landing at E-end of Robertson Bridge over Rogue R. 307 18 2 Oregon Josephine 6W. 35S 30 Pasture 1. 2 mi. N of Robertson Bridge on Merlin-Robertson Bridge Rd.

308 18 1 Oregon Josephine 6W 35S 22 RR embankment adjacent to Merlin- Galice Rd,1.4 mi. W of junction with Imerstate Hwy, 5,

309 27 2 Oregon Douglas 6W 32S 35 Open hillside W-side of Glendale- Azalea Rd.0. 8 mi. N of junction with Interstate Hwy. 5. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

310 27 1 Oregon Douglas 6W 32S 25 Open valley pasture, Azalea Rd. 1. 4 mi. N of junction with Interstate Hwy. 5. 311 27 1 Oregon Douglas SW 30S 33 Fence-row, Riddle Canyonville Rd. 2. 1 mi. W of junction with Interstate Hwy. 5. 312 27 1 Oregon Douglas 6W 30S 24 E-side of Riddle-Canyonville Rd.0. 8 mi. S of Riddle city limits. 313 27 2 Oregon Douglas 6W 30S 12 Top of roadside cut, opposite sawmill, 1. 1 mi. W of Interstate Hwy. 5 on Riddle-Tri City Rd. 314 18 1 Oregon Douglas SW 25S 31 Interstate Hwy. 5 at SE-end of cut bank 2. 9 mi. S of Sutherlin exit ramp.

315 18 1 California Siskiyou 7W 46N 13 Achenes collected from plants on bank above Calif. Hwy. 96, 0. 8 mi. W of junction with U. S. Hwy. 99. 316 18 1 California Siskiyou 7W 46N 14 0.6 mi. E of Klamath Nat. For, boundary marker on Calif. Hwy. 96; achenes, Klamath R. canyon. 317 18 1 California Siskiyou 9W 46N 12 Bank above Klamath R. on Calif. Hwy. 96, 1.9 mi. E of Oak Knoll Ranger Station; achenes collected.

318 18 1 California Siskiyou 8W 46N 1 Klamath R. bank adjacent to Calif. Hwy.96, 0. 3 mi. E of Empire Creek bridge. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

319 18 1 California Siskiyou tOW 46N 1S S-side of Klamath R. bridge on W- facing bank under forest canopy on Calif. Hwy. 96, 0. 9 mi. E of Horse Creek bridge.

320 18 1 California Siskiyou 1.1W 46N 35 1. 3 mi. W of Hamburg city limit sign on Calif. Hwy. 96 and 0.3 mi. W of Mill Creek bridge; by abandoned garage.

321 18 1 California Siskiyou 11W 46N 18 Achenes, roadside bank of Calif. Hwy. 96, 0. 8 mi. E of Siead city limit sign; Klamath R. valley. 322 18 1 California Siskiyou SE 16N 5 Achenes, 0. 2 mi. E of Slater Butte L. O. Rd. junction with Calif. Hwy. 96. Klamath R. valley 4.5 mi. E of Happy Camp.

323 18 1 California Siskiyou 8E 16N 6 Achenes, 2.5 mi. E of Brown's Creek bridge and 9. 8 mi. W of Happy Camp on Calif. Hwy. 96; Klamath R. valley. 324 18 1 California Siskiyou 6E 12N 21 Achenes from bank along old route of Calif. Hwy. 96, 2. 5 mi. E of Somes- bar Post Office. Klamath R. canyon.

325 18 1 California Humboldt 6E 11N 9 Achenes, Calif. Hwy. 96 bank, 6. 4 mi. E of Orleans city limit sign and 2. 9 mi. W of Somesbar. Klamath R. canyon.

326 18 1 California Humboldt 5E 10N 30 Achenes, Calif. Hwy. 96 roadside 0. 3 mi. E of Bluff Creek Bridge. Klamath R. valley. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

327 27 1 California Humboldt 4E 9N -- Achenes from plants under forest canopy, E-facing bank of Calif. Hwy. 96, 13. 1 mi. W of Cedar Camp Rd.

328 27 1 California Shasta 7W 33N 30 Achenes, Calif. Hwy. 299 roadside bank at turnout 0.1 mi. W of 2000 ft. elevation marker; E-side of pass.

329 27 1 California Fresno 22E 14S 12 Achenes, from irrigation canal bank intersecting Calif. Hwy. 180 at New Mark Rd.Plants more than four feet tall.

330 18 1 California Mono 25E 2N 31 0,5 mi. E of U. S. Hwy. 395 at Mono Lake. E of Sierras.A. Legge. 331 27 5 California Fresno 26E 14S 7 Large clone at edge of barn feedlot on Ruth Hill Rd.5.7 mi. W of Dun- lap; opposite Stoddard Ranch house; hills at E-end of Squaw valley.

332 2n = 45 1 California Fresno 26E 14S 3 Achenes, bank of old Calif. Hwy. 180 at W-side of Dunlap residential area. E of Squaw Valley in surrounding hills. 333 27 2 California Tulare 24E 16S 6 Irrigation ditch bank crossing Alta Rd. at Tulare-Fresno County line.

334 18 1 California San Bernardino 2E 2N 6 Small population in marshy area of dry Baldwin Lake bed along Calif. Hwy. 18, E of Big Bear Lake city limits. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

335 18 2 Utah Garfield 4W 36S 36 Scattered plants at Sunset Point ob- servation area; Bryce Canyon National Park.

336 27 1 Oregon Clatsop low 7N 34 1. 0 mi. N of Gearhart city limits at termination of four-lanes of U. S. Hwy. 101, roadside ditch. 337 27 2 Oregon Clatsop 10W 8N 28 Embankment at E-end of Skipanon R, bridge on U. S. Hwy. 101.

338 18 1 WashingtonPacific 10W 9N 24 River bluffs, N-end of Columbia R. - Astoria Toll bridge at junction of U. S. Hwy. 101 and Wash. Hwy. 401.

339 27 2 WashingtonPacific 10W 9N 6 Roadside of U. S. Hwy. 101, 5. 1 mi. S of liwaco city limit sign.

340 27 1 WashingtonPacific 10W 11N 29 7.6 mi. S of junction with Wash. Hwy. 4 on U. S. Hwy. 101 opposite bay and mudflats. 341 27 2 WashingtonPacific 9W 14N 27 U. S. Hwy. 101, N-edge of South Bend opposite Redi-Gas facility. 342 27 2 WashingtonPacific 9W 14N 16 Roadside ditch of Wash. Hwy. 105, 3. 5 mi. W of Raymond city limit sign.Wilapa R. flood plain. 343 27 2 WashingtonPacific 10W 15N 33 Slough bank 2. 5 mi. W of North R. bridge on Wash. Hwy. 105. 344 27 2 WashingtonGrays Harbor 12W 16N 25 Ditch at residential driveway on Wash. Hwy. 105, 2.5 mi. S of Westport city limits. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County RangeTownship Section Notes

345 18 1 WashingtonGrays Harbor 11W 16N 2 Waste area at E-end of Johns R. bridge on Wash. Hwy. 105. 346 18 2 WashingtonGrays Harbor 9W 17N 19 Large population along Wash. Hwy. 105, bay plain 1. 3 mi. W of Aber- deen city limits opposite Aberdeen Auto Wreckers business.

347 27 1 WashingtonGrays Harbor lOW 17N 4 Opposite Hoquiam H. S. in ditch of Wash. Hwy. 109 at W-edge of Hoquiam.

348 18 2 WashingtonGrays Harbor 11W 18N 15 0. 2 mi. W of Chenois Creek bridge along roadside of Wash. Hwy. 109.

349 27 1 WashingtonGrays Harbor 12W 18N 22 1.0 mi. N of Ocean City State Park entrance and 0. 3 mi. S of Wash. Hwy. 109 on park rd.

350 27 1 WashingtonGrays Harbor 12W 19N 27 Water-filled ditch, 0.1 mi. S of Copalis city limits on Wash. Hwy. 109. 351 27 2 WashingtonGrays Harbor 12W 19N 23 Bank of Copalis-Humptulip Rd. 1.1 mi. E of intersection with Wash. Hwy. 109. 352 27 2 WashingtonGrays Harbor 11W 19N 28 Roadside of Copalis-Humptuli Rd. 0. 7 mi. E of Copalis Crossing. 353 27 2 WashingtonGrays Harbor lOW 20N 9 Roadside of U. S. Hwy. 101, 1. 1 mi. N of Humptulip-Copalis Rd. junction. Appendix I Continued, Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

354 27 1 WashingtonGrays Harbor lOW 22N 26 Small population, open area adjacent to U. S. Hwy. 101, 1. 2 mi. N of Olympic Nat. For, boundary marker.

355 27 1 WashingtonGrays Harbor 11W 23N 23 Roadside U. S. Hwy. 101, 6.2 mi. S of Raft R. bridge and Jefferson- Grays Harbor Co. line marker. 356 18 2 WashingtonJefferson 13W 24N 10 Trail to beach opposite Kalaloch Ranger Station, Olympic Nat. Forest. 357 18 2 WashingtonJefferson 13W 25N 17 Beach parking lot No. 6 margin, Kalaloch Beach on U. S. Hwy. 101, S. 9 mi. N of Kalaloch Ranger Station.

358 27 WashingtonJefferson 12W 26N 8 Roadside of U. S. Hwy. 101, 3.3 mi. S of Hoh R. bridge.

359 18 1 WashingtonJefferson 13W 27N 12 2, 0 mi. S of Jefferson-Clallam Co. line marker on U. S. Hwy. 101. Open roadbank. 360 18 2 WashingtonClallam 13W 28N 21 Roadside of U. S. Hwy. 101, 4.1 mi. N of Jefferson-Clallam Co. line marker. 361 18 2 WashingtonClallam 13W 29N 28 0.6 mi. N of Soleduck R. bridge along widened right of way of U. S. Hwy. 101.

362 18 1 WashingtonClallam 12W 30N 20 On Sappho-Neah Bay Rd. 1. 5 mi. N of junction with U. S. Hwy. 101 at Beaver Creek bridge. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

363 18 2 WashingtonClallam 12W 30N 9 Shore of Beaver Lake on Sappho -Neap Bay Rd. by forest service outhouses.

364 18 1 WashingtonClallam 12W 31N 14 Wash. Hwy. 112, 0. 2 mi. E of junction with Sappho-Neah Bay Rd. 365 18 2 WashingtonClallam 11W 31N 14 Roadside of Wash. Hwy. 112, 0. 3 mi. W of Merritt-Ring State Recreational Area. 366 18 2 WashingtonClallam lOW 31N 23 0. 3 mi. E of West Twin River bridge on Wash. Hwy. 112.

367 18 1 WashingtonClallam 9W 31N 27 1. 1 mi. E of Lyre R. bridge at farm road intersection on Wash. Hwy. 112.

368 18 1 WashingtonClallam 8W 30N 2 1. 3 mi. E of Salt Creek bridge at roadside of Wash. Hwy. 112. 369 18 3 WashingtonClallam 4W 30N 19 Steep roaciside, cut on U. S.. Hwy. 101, 0. 1 mi. E of McDonnell Creek bridge; just above Texaco gas station. 370 18 2 WashingtonClallam 3W 29N 2 Waste area behind abandoned buildings adjacent to U. S. Hwy. 101, 0.1 mi. W of Blyn city limit sign.

371 18 1 Washington",Jefferson 2W 29N 1 Turnout on U. S. Hwy. 101, 3.5 mi. S of Jefferson-Clallam Co. line marker.

372 18 1 WashingtonJefferson 2W 26N 35 Waste area by house along U. S. Hwy. 101, at Elkhorn Camp Rd. junction. Appendix I Continued. Accession Chromosome Number of number numberjn) plants counted State County Range Township Section Notes

373 18 1 WashingtonMason 3W 23N 9 Achenes, waste area adjacent to U. S. Hwy. 101, 3. 4 mi. S of Hamma Hamma R. bridge and 0. 5 mi. N of Eagle Creek bridge.

374 18 1 WashingtonMason 3W 23N 17 Achenes, roadside bath of U. S. Hwy. 101, 0.6 mi. S of Eagle Creek bridge.

37S 18 1 WashingtonMason 4W 22N 23 Bank of U. S. Hwy. 101, 1. 8 mi. S of Hoodsport and 0. 2 mi. N of Pot- hatch.

376 27 1 Washington Mason 4W 20N 24 W-edge of Shelton, Wash. 0. 2 mi. E of Shelton Valley Rd. turnoff on Matlock-Shelton Rd.

377 27 1 Washington Mason 4W 20N 22 Matlock-Shelton Rd. 3. 5 mi. E of Dayton city limit sign.

378 2n = 45 1 Washington Mason 4W 20N 18 Fence row opposite Dayton community hall.

379 27 1 WashingtonGrays Harbor 7W 18N 12 Matlock-Satsop Rd. 1.6 mi. S of Mason-Grays Harbor Co. line marker. 380 27 2 WashingtonGrays Harbor 6W 18N 32 Margin of Wash. Hwy. 112, 2.1 mi. E of intersection with Matlock-Satsop Rd. between RR embankment and hwy.

381 27 2 WashingtonGrays Harbor SW 18N 2 2. 0 mi. E of McCleary city limit sign on Wash. Hwy. 108. 382 27 2 WashingtonMason 4W 19N 28 Large population on steep bank 2.0 mi. E of Grays Harbor-Mason Co. line marker on Wash. Hwy. 108. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

383 27 1 WashingtonMason 4W 19N 24 Wash. Hwy. 108, 1. 2 mi. W of junction with U. S. Hwy. 101 and 0. 2 mi. W of RR crossing.

384 27 2 WashingtonSnohomish 4E 28N 34 Roadside of U. S. Hwy. 99, 0. 2 mi. S of Wash. Hwy. 525 junction, suburban Seattle. 385 18 2 WashingtonIsland 2E 30N 33 Opposite county dump 5.0 mi. S of Greenbank city limit sign on Wash. Hwy. 525.Whidbey Island.

386 18 1 Oregon Deschutes 9E 21S 20 Achenes, N of Wickiup Dam, ca. 10 mi. E of U. S. Hwy. 97 on BLM Rd. 204.W. L. Ezell. 387 18 2 WashingtonIsland 2E 30N 21 Turnout, abandoned house yard on Wash. Hwy. 525, 2. 2 mi. S of Green- bank city limits. Whidbey Island.

388 18 2 WashingtonIsland 2E 31N 32 Wash. Hwy. 525 at unmarked intersection 2.0 mi. N of Greenbank city limits.Whidbey Island. 389 18 2 WashingtonIsland lE 32N 36 Waste area along Wash. Hwy. 525, opposite Rhododendron Park 4.0 mi. N of junction with Wash. Hwy. 113. Whidbey Island.

390 18 1 WashingtonIsland 1E 32N 16 Open fields 2. 4 mi. S of Oak Harbor city limits on Wash. Hwy. 525. Whid- bey Island. Appendix I Continued. Accession Chromosome Number of number number .lants counted State CO= Ran e Townshi. Section Notes

391 18 1 WashingtonIsland 1E 33N 2 2.0 mi. S of Deception Pass bridge on Wash. Hwy. 525 at Banta Rd. intersection. Whidbey Island. 392 18 2 WashingtonSkagit 2E 34N 7 Large population along Wash. Hwy. 536, 0. 5 mi. E of Wash. Hwy. 525 junction.Opposite Texaco refinery.

393 18 1 WashingtonSkagit 3E 34N 7 Small population at edge of Wash. Hwy. 536, 1. 2 mi. E of Swinomish Slough bridge.

394 18 1 WashingtonSkagit 3E 35N 32 Margin of cultivated field, 2. 1 mi. N of junction with Wash. Hwy. 536 on Wash. Hwy. 537. 395 18 2 WashingtonSkagit 3E 36N 33 Edison, Wash.Marshy slough directly in front of Edison elementary school.

396 18 1 WashingtonWhatcom 2E 37N 1 Achenes, bay bluff bank at intersection of State Ave. and Chuckanut Drive; urban Bellingham, Wash. 397 18 2 WashingtonWhatcom 2E 39N 31 Drainage ditch adjacent to county road to Mountain View 0.1 mi. W of termination of Wash. Hwy. 540.

398 18 1 WashingtonWhatcom 1E 41N 6 Waste area, opposite Chuck's Drive-In diner. 13, Peace Arch Blvd. Blaine, Wash. 399 27, 36 1, British -- Population along fence row and ditch 2n = 63 1 Columbia at intersection of New McClellan Rd. and Princess St., residential Langley, B. C. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

400 18 2 British ------Trans-Canada Hwy. 1 ca. 1. 5 mi. E Columbia of Langley city limits and 50 yd. W of Fort Langley Historical Marker sign. 401 27 2 British ...... -- ..... Waste area N-side of Esso Gas Station Columbia at junction of B. C. Hwy. 113 and Trans-Canada Hwy. 1. 402 27 1 WashingtonWhatcom 4E 40N 16 Fence row, Wash. Hwy. 9, O. 7 mi. N of junction with Wash. Hwy. 544. 403 18 1 WashingtonWhatcom 4E 39N 8 3. 5 mi. S of Wash. Hwy. 544 junction on Wash. Hwy. 9; roadside in farming area. 404 27 1 WashingtonWhatcom SE 38N 30 Culvert bridge embankment, Wash. Hwy. 9, 4. 1 mi. S of junction with Wash. Hwy. 542. 405 1 WashingtonWhatcom 5E 37N 20 Roadside of Wash. Hwy. 9, 3. 2 mi. S of Acme city limit sign and 300 ft. S of Ranch Tavern. 406 18 2 WashingtonSkagit 4E 36N 13 3.0 mi. S of Whatcom-Skagit Co. line marker at roadside of Wash. Hwy. 9. 407 18 2 WashingtonSkagit 4E 36N 24 RR embankment at Wash. Hwy. 9 crossing 4. 5 mi. S of Skagit-Whatcom line marker.

408 27 1 WashingtonSkagit SE 35N 18 Residential area, Sapp Rd. intersection with Wash. Hwy. 9, 0. 5 mi. N of Sedro Woolley city limit sign. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

409 27 2 WashingtonSnohomish 5E 31N 2 Railroad right of way opposite West St. and Gilman Rd. intersection suburban Arlington.

410 27 1 WashingtonSnohomish SE 31N 11 Residential area 0.1 mi. S of Wash. Hwy. 530 on Wash. Hwy. 9; S Arlington.

411 27 1 WashingtonSnohomish 6E 29N 9 Lake Stevens Rd. intersection with Wash. Hwy. 9, 1. 3 mi. N of junction with Wash. Hwy, 204, 412 18 2 WashingtonClallam 6W 29N 27 Mt. Angeles, Olympic Nat. Forest. Elev. 6000 ft.A. Legge.

413 18 1 WashingtonClallam 6W 29N 21 Mt. Angeles trail, Olympic Nat. Forest.Elev. 6200 ft.A. Legge.

414 18 1 WashingtonJefferson 6W 29N 30 Hurricane Ridge trail, Olympic Nat. Park.Elev. 5900 ft.A. Legge.

415 18 1 WashingtonYakima 19E 8N 6 Achenes, N.-side of Satus Pass, U.S. Hwy. 97 at bridge over Satus Creek. K. L. Chambers.

416 18 1 Oregon Wasco 12E 2N 11 Achenes, Rowers exit of Interstate Hwy. 80.Columbia R. flood plain bluffs.R. L. Carr.

417 18 1 Oregon Clackamas 7E 3S 11 Achenes, just S of Still Creek camp-. ground.Mt. Hood area,R. L. Carr. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

418 27 1 Oregon Clatsop lOW 8N 29 On jeep trail to the ocean, off of road from U. S. Hwy. 101 to Fort Stevens. Achenes only. Ammophila reclaiming dune area with Acbillea in thick stand on lee side of foredune. K. L. Chambers. 419 27 2 WashingtonPacific 10W 9N 6 U. S. Hwy. 101, 9.0 mi. N of Astoria bridge over Columbia R. Low marshy area near Chinook. K. L. Chambers. 420 27 3 WashingtonPacific 11W 13N 27 Ledbetter Pt. N of Oysterville 0. 3 mi. from turnaround at end of graveled rd.Bayside marshes and sandy soil.K. L. Chambers. 421 27 2 Oregon Clatsop 9W 5N 19 U. S. Hwy. 26, 4. 9 mi. W of Saddle Mt. Rd. junction. Low ditch adjacent to hwy. K. L. Chambers. 422 18 3 Oregon Douglas 6W 27S 12 Large population along fence row of Interstate Hwy. 5, 200 yds. N of Roseburg-Garden Valley exit ramp. 423 27 2 Oregon Douglas 6W 28S 24 Scattered plants, W bank of Interstate Hwy. 5, 5.5 mi. N of S Fork of Umpqua R. bridge. 424 27 3 Oregon Douglas SW 29S 19 Small population 0. 5 mi. N of Boomer Hill Rd. exit ramp behind signpost on Interstate Hwy. 5, W-side; ca. 1.7 mi. N of Myrtle Creek. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

42S 18 1 Oregon Josephine SW 36S 5 0. 5 mi. N of Grants Pass exit ramp on Interstate Hwy. 5; small popula. tion on. S-side embankment. 426 18 2 Oregon Josephine 6W 36S 19 Waste area, public boat landing on Robertson Bridge-Whitehorse Park Rd. ca. 3.0 mi. W of Grants Pass city limits. 427 18 2 Oregon Josephine 6W 36S 11 Robertson Bridge-Whitehorse Park Rd., cut bank 300 ft. E of Carl Womack driveway, 1.7 mi. E of Whitehorse Park entrance. 428 18, 1, Oregon Josephine 6W 36S 18 Gunnel Rd. to Matson Park, junction 27 1 with Robertson Bridge-Whitehorse Park Rd. 429 18 2 Oregon Josephine 6W 36S 6 Small population, RobertsonBridge- Whitehorse Park Rd. at Hugo-Merlin Rd. junction.

430 18 1 Oregon Josephine 7W 36S 11 Isolated plant at side of Robertson Bridge-Wilderville Rd.O. 3 mi. S of Griffen Park Rd. intersection. 431 27 2 Oregon Josephine 7W 37S 10 U. S. Hwy 199, 1.0 mi. N of Wonder, Ore, at edge of oak woods. 432 27 1 Oregon Josephine 7W 37S 17 Waste area at side of tavern-cafe on U. S. Hwy. 199, 1. 3 mi. S of Won- der, Ore. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range TownshipSection Notes

433 27 2 Oregon Josephine 8W 38S 33 Fence row population 0. 2 mi. S of Reeves Creek bridge on U. S. Hwy. 199, Illinois R. valley.

434 27 2 Oregon Josephine 8W 39S 32 Waste area at entrance to Gibbons Market 5.0 mi. S of Cave Junction city limit on U. S. Hwy. 199, Illinois R. valley.

435 27 2 Oregon Josephine 8W 40S 8 Scattered plants, E-side of U. S. Hwy. 199, 0. 2 mi. N of Airport Rd. intersection.Illinois R. valley. 436 27 2 Oregon Josephine 8W 40S 19 Fence row, small population, O. 1 mi. E of U.S. Hwy. 199 on Waldo-O'Brien Rd., Illinois R. valley.

437 27 2 Oregon Josephine 8W 40S 26 Isolated plants at ends of bridge over E Fork of Illinois R., Waldo O'Brien Rd. Embankment fill.

438 27 3 Oregon Josephine 8W 40S 27 Large population on hillside behind Waldo historical marker.Serpentine soil.

439 27 1 Oregon Josephine 8W 39S 34 Rockydale Rd. 1. 3 mi. S of junction with U. S. Hwy. 199,Isolated plant behind mailbox of F. E. Thayer, Illinois R. valley.

440 27 1 Oregon Josephine 8W 39S 21 Single plant behind Oregon Caves junction marker sign.S-edge of Cave Junction, Ore. Appendix I Continued. Accession Chromosome Number of number number (u) plants counted State County Range Township Section Notes

441 27 2 Oregon Josephine 8W 39S 16 Roadside, 200 ft. N of Laurel Rd. intersection on U. S. Hwy. 199. Illinois R. valley. 442 27 2 Oregon Josephine 8W 38S 11 Fence row, 1.2 mi. E of U.S. Hwy. 199 on Selmac Lake Rd., Illinois R. valley. 443 18 2 Oregon Josephine 6W 36S 30 Wilderville-Robertson Bridge Rd., 2.0 mi. N of junction with U. S. Hwy. 199.Fence row, Rogue R. valley bluffs. 444 18 2 Oregon Josephine 6W 36S 30 Clear area 2.3 mi. N of U. S. Hwy. 199 on Wilderville-Robertson Bridge Rd., old bluffs of Rogue R. 445 18, 2, Oregon Josephine 6W 36S 19 Edge of driveway, Harold Slayson 2n =45 1 No. 3081, Wilderville-Robertson Bridge Rd., 0. 3 mi. S of Marcy Loop Rd. 446 27 1 Oregon Josephine 7W 36S 14 Isolated plant, fence row, Wilder- ville-Robertson Bridge Rd., 0.6 mi. N of Marcy Loop Rd. junction. 447 18 3 Oregon Josephine 7W 35S 26 Bluff above E end of Robertson Bridge over Rogue R., large population. 448 18 3 Oregon Josephine 7W 35S 25 Fence row 0. 3 mi. N of Artlin Rd. junction on Merlin-Robertson Bridge Rd.scattered plants. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

449 18 2 Oregon Josephine 6W 35S 30 Entrance to driveway, Robertson Bridge Rd. RFD No. 12686.Large population in field 0.6 mi. N of Art lin Rd. intersection. 450 18 1 Oregon Josephine 6W 35S 30 Open field, 0.9 mi. N of Art lin Rd. on Robertson Bridge-Merlin Rd. 451 18 1 Oregon Josephine 6W 35S 20 Single isolated plant at junction of Robertson Bridge Rd. and Azalea Rd., 1.0 mi. S of intersection of Robert- son Bridge Rd. and Merlin - Galice Rd. 452 18 2 Oregon Josephine 6W 35S 20 Open field 0.2 mi. E of Hugo Rd. intersection on Merlin-Galice Rd., rural housing area, disturbed ground. 453 18 2 Oregon Josephine 8W 34S 36 0.5 mi. N of Galice on hillside above Merlin-Galice Rd. Large population, Rogue-R. canyon. 454 18 2 Oregon Josephine 8W 34S 36 Merlin-Galice Rd.1.0 mi. NW of Galice; Roadside gravel; scattered plants; Rogue R. canyon. 45S 18 2 Oregon Josephine 8W 34S 24 1.0 mi. N of Rand Ranger Station on Merlin-Galice Rd.; Rogue R. canyon.

456 18 1 Oregon Josephine 7W 34S 3 Lower Grave Creek Rd., 6.0 mi. E of Rogue R. bridge; isolated plant in waste area. 457 18 2 Oregon Josephine 7W 34S 2 Large population along roadside of Lower Grave Creek Rd., 6.5 mi. E of Rogue R. bridge. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

458 18 1 Oregon Josephine 7W 34S 2 Small population, roadside of Lower Grave Creek Rd. 2.0 mi. W of junction with Wolf Creek and Leland Rds.

459 2n = 45 1 Oregon Josephine 7W 33S 36 Roadside bank of Lower Grave Creek Rd. 0.3 mi. W of junction with Wolf Creek and Leland Rds.At edge of forest clearing. 460 18 2 Oregon Josephine 7W 33S 36 Small population, waste area 0.2 mi. E of junction with Leland Rd. and Lower Grave Creek Rd. on Wolf Creek Rd.

461 18 1 Oregon Josephine 6W 33S 30 Small population 1.8 mi. E of Leland Rd. and Lower Grave Creek Rd. junction on Wolf Creek Rd. 462 27 2 Oregon Josephine 6W 33S 21 Between Wolf Creek Rd. and RR track embankment 2.3 mi. E of Leland- Lower Grave Creek Rds, junction and 2.5 mi. W of Wold Creek, Ore. Opposite RFD No. 2389 mailbox.

463 27 1 Oregon Josephine 6W 33S 15 Old U. S. Hwy. 99 into Wolf Creek, Ore, directly opposite end of S off ramp of Interstate Hwy. 5. 464 27 2 Oregon Douglas 6W 32S 26 Top of roadside cut bank 0.2 mi. N of Azalea-Glendale Rds. junction on Azalea Rd.

465 27 1 Oregon Douglas 6W 32S 25 Scattered plants Azalea Rd. 1.0 mi. N of Azalea-Glendale Rds. junction. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

466 27 2 Oregon Douglas 6W 32S 25 Large population along fence row adjacent to Azalea Rd. 1.6 mi. N of Azalea-Glendale Rds. junction. 467 27 3 Oregon Douglas SW 32S 19 Drainage ditch adjacent to Azalea Rd. (Old Hwy. ) 2.5 mi. N of Azalea- Glendale Rds. junction. 468 27 1 Oregon Douglas 5W 32S 21 4.5 mi. N of Azalea-Glendale Rds. junction on Azalea Rd. (Old Hwy.); roadside bank. 469 27 2 Oregon Douglas 5W 32S 15 Roadside bank cut, 0.7 mi. S of Interstate Hwy. 5 junction on Azalea Rd.(Old Hwy. ) 470 27 2 Oregon Douglas SW 30S 33 Canyonville-Riddle Rd. 2.0 mi. W of Interstate Hwy. 5 exit ramp. Fence row, small population. 471 27 2 Oregon Douglas 5W 30S 30 Large population along banks of road fill on Canyonville-Riddle Rd. 2.6 mi. W of Interstate Hwy. 5 exit ramp. 472 27 2 Oregon Douglas SW 30S 30 Bank behind county water tower on Canyonville-Riddle Rd. 3.4 mi. W of Interstate Hwy. 5 exit ramp. 473 27 4 Oregon Douglas 6W 30S 14 Junction of Glendale Loop Rd. and Riddle Rd. Large population on banks at roadside. 474 27 3 Oregon Douglas 6W 30S 14 0.5 mi. E of Riddle-Canyonville Rd. on Glendale Loop Rd.Large population in fields. Co Appendix I Continued. Accession Chromosome Number of number number (al) plants counted State County Range Township Section Notes

475 27 1 Oregon Douglas 6W 30S 12 Cut banks of roadside 1. 2 mi. E of Riddle-Canyonville Rd. on Glendale Loop Rd. 476 27 2 Oregon Douglas 6W 30S 12 Bank above Glendale Loop Rd. 100 yds. W of RR crossing.Scattered plants. 477 18 2 California Del Norte 3E 17N 10 Small population, S-facing bank of roadside cut, U.S. Hwy. 199, 3. 9 mi. E of Gasquet Ranger Station. 478 27 3 California Del Norte 2E 17N 20 Flood plain of Smith R.Large population, intersection of Gasquet Flat Rd. and Gasquet Flat Side Rd. Behind power substation. 479 27 1 California Humboldt 4E 4S 17 Single isolated plant, edge of Garber- ville-Harris Rd. 1. 1 mi. E of Garber- ville-Redwood junction. Open grasslands with scattered oaks. 480 27 3 California Humboldt 2E 2N 31 Roadside of Calif. Hwy. 36, 200 ft. E of Carlotta Fire Station #2.Approx. 1. 0 mi. W of Strongs Station. 481 27 3, California Humboldt 3E 1N 8 S-facing roadside bank on Calif. Hwy. 2n = 45 1 36, 5. 2 mi. E of Grizzly Creek campground entrance. 482 27 2 California Humboldt 3E 1N 14 Kneeland-Bridgeville Rd. O. 3 mi. N of Bridgeville.Bluffs overlooking town. Edge of oakwoods.Scattered plants. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

483 27 1 California Humboldt 3E 1N 23 Single plant, bank cut 0. S mi. SW of Bridgeville on Alderpoint-Bridgeville Rd.

484 27 2 California Humboldt 3E 1N 13 Calif. Hwy. 36, 0.6 mi. W of Bridgeville city limit sign. 485 27 2 California Humboldt 3E 1N 22 1. 0 mi. W of Bridgeville city limit sign on Calif. Hwy. 36.Edge of oak- conifer woods; single plant in gravel margin or road. 486 27 2 California Humboldt 5E 7N 20 Calif. Hwy. 96, 1. 1 mi. N of Willow Creek bridge; waste area. 487 18 2 California Humboldt 5E 7N 5 Top of cut banks above Calif. Hwy. 96, 7. 2 mi. N of Willow Creek bridge. 488 18 2 California Humboldt 4E 8N 26 Residential area, Hoopa, Calif. 0. 5 mi. N of Trinity R. bridge on Calif. Hwy. 96.Waste area opposite Union 76 gas station. 489 27 2 California Humboldt 4E 9N 35 W-facing bank above Calif. Hwy. 96, 5. 3 mi. N of Hoopa city limit sign.

490 27 3 California Humboldt SE lON 30 0. 5 mi. N of Bluff Creek Rd. on Calif. Hwy. 96.Banks of road cut. 1. 5 mi. S of Bluff Creek campground entrance.

491 27 1 California Humboldt 5E iON 10 Calif. Hwy. 96, 2. 8 mi. S of Orleans city limit sign; SE-facing bank. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

492 18 2 California Humboldt 6E 11N 16 4, 7 mi. N of Klamath R. bridge at Orleans, Calif. on Calif. Hwy. 96. 493 18 2 California Humboldt 6E 11N 10 Calif. Hwy, 96, 6. 8 mi. N of Orleans and Klamath R. bridge; 0. 2 mi. S of Humboldt-Siskiyou County line.

494 18 2 California Siskiyou 6E 11N 1 Salmon R. Rd. 2.2 mi. E of junction with Calif. Hwy. 96, 495 18 2 California Siskiyou 7E 11N 22 Salmon R. Rd. 8. 5 mi. E of junction with Calif. Hwy. 96; 2, 8 mi. W of Lewis Creek bridge. 496 18 2 California Siskiyou 8E lON -- 3. 1 mi. W of Forks of Salmon; 0. 3 mi. E of Salmon R. bridge on Salmon R. Rd. 497 18 2 California Siskiyou 12W 39N 19 Scattered plants at edge of Cecilville Rd. 3.1 mi. E of Forks of Salmon. 498 18 2 California Siskiyou 7W 46N 36 Roadside of U. S. Hwy. 99, 2.3 mi. S of Klamath R. bridge in Shasta R. canyon. Large population on steep cut banks. 499 18 3 California Siskiyou 6W 46N 5 Klamath R. Rd. at entrance to Inter- state Hwy. 5, 2. 5 mi. N of Klamath R. bridge.E-facing bank. 500 18 3 California Siskiyou 7W 48N 13 Roadside of U. S. Hwy. 99, 300 ft. S of SP railroad overpass; 1. 1 mi. S of Hilts, Calif. Appendix I Continued. Accession Chromosome Number of number number n ants counted State Count Ran eTownshi. Section Notes

501 18 1 Oregon Jackson lE 39S 13 Siskiyou Rd. exit ramp off Interstate Hwy. 5; Ashland, Ore. Large clonal clump. 502 18 2 Oregon Jackson 4W 36S 27 Large population, N-side Interstate Hwy. 5, 1.6 mi. S of Rogue River, Ore, exit ramp. 503 27 2 Oregon Douglas SW 31S -- Interstate Hwy. 5 fencerow, 400 ft. S of Stanton Rd. turnoff sign; E-side of freeway. 504 27 3 Oregon Douglas 5W 30S 18 Base of bluffs of S. Umpqua R. valley, E-side of Interstate Hwy. 5, 1.0 mi. S of Tri City exit ramp.

505 27 3 Oregon Douglas 6W 28S 11 Banks E-side of Interstate Hwy. 5, 2.1 mi. S of Ore. Hwy. 42 junction.

506 27 1 Oregon Douglas 6W 26S 24 Underpass Winchester-N.Roseburg exit, E-side of Interstate Hwy. 5.

507 18 1 Oregon Josephine 5W 36S 16 Interstate Hwy. 5, 1.0 mi. N of N. Grants Pass exit ramp; opposite information, S-side at top of cut bank. 508 18 1 Oregon Jackson 4W 36S 30 South side of Interstate Hwy. 5, 1.0 mi. S of Josephine-Jackson Co. line sign; large population.

509 18 1 Oregon Jackson 3W 36S 21 Small population 0.6 mi. N of Jack- sonville-Gold Hill Rd. exit ramp on Interstate Hwy. 5; beneath large oak tree. co N.) Appendix I Continued. Accession Chromosome Number of number number n lanes counted State Count Ran eTow hi Section Notes

510 18 2 Oregon Jackson 3W 36S 27 Old Stage Rd. 0. 8 mi. W of junction with Interstate Hwy. 5; open field along fence row.

511 18 2 Oregon Jackson 2W 37S 16 Old Stage Rd. 3. 6 mi. N of Jackson- ville Post Office and 1. 1 mi. N of Hillside Rd. intersection.Fence row with Arctostaphylos. 512 27 2 Oregon Jackson 2W 37S 32 Jacksonville-Phoenix Rd. 0. 5 mi. E of Jacksonville; fence row at edge of madrone woods.

513 18 2 Oregon Jackson 6W 40S 9 W-side of Interstate Hwy. 5, 300 yd. N of Southern Pacific RR overpass.

514 18 1 California Siskiyou 7W 48N 25 Waste area, W-side of U. S. Hwy. 99, 0. 3 mi. S of Calif. Quarantine Station.

515 18 1 California Siskiyou 6W 46N 19 U. S. Hwy. 99, Shasta R. canyon O. 3 mi. S of first bridge over Shasta R. following junction with Klamath R.

516 18 2 California Siskiyou 7W 45N 34 0. 3 mi. S of junction with U. S. Hwy. 99 on Calif. Hwy. 3.Edge of feed lot opposite lumber mill entrance, S-side of Yreka.

517 18 2 California Siskiyou 7W 44N 7 Dry gulch banks on Calif. Hwy. 3, 3. 2 mi. S of U. S. Hwy. 99 junction. Elev. 3100 ft.Just below Pass.

518 18 1 California Siskiyou 8W 44N 33 Small clone, side of Calif. Hwy. 3.

N-end of Scott R. valley 2.0 mi. S oo of Moffett Creek bridge. r.a Appendix I Continued. Accession Chromosome Number of number number n lants counted State Court. Range Township Section Notes

519 18 2 California Siskiyou 9W 43N 2 400 ft. N of Scott R. bridge on Calif. Hwy. 3, 1.0 mi. S of Fort Jones city limit sign in ditch. 520 18 2 California Siskiyou 9W 42N 17 Fence row adjacent to Calif. Hwy. 3, 2. 3 mi. N of Etna, Calif.Large population in grasslands.

521 27 1 California Siskiyou 9W 42N 32 Residential area, W-side of Etna,, Calif.Fence row on Etna-Sawyer's Bar Rd.

522 18 1 California Siskiyou low 41N Clearing Etna-Sawyer's Bar Rd. 500 W of Cow Creek.3. 2 mi. E of Idle- wild campground and 16. 7 mi. W of Etna.Dense coniferous forests.

523 18 1 California Siskiyou 10W 40N 17 Open area Etna-Sawyer's Bar Rd. 0. 4 mi. E of Snoozer Ridge and Yellow Dog Peak trail and 0.6 mi. E of Idlewild campground. 524 18 2 California Siskiyou 12W 40N 24 Sawyer's Bar-Forks of Salmon Rd. 0. 7 mi. W of Kelley Creek bridge. Banks of N. Fork of Salmon R. 0. 5 mi, E of Little N. Fork Public camp. 525 18 2 California Siskiyou 12W 40N 32 Sawyer's Bar-Forks of Salmon Rd. 4.6 mi. W of Little N. Fork Public Camp. Dense forests with scattered plants along road only. 526 18 2 California Siskiyou 8E 10N 7 0.1 mi. E of Pollock Creek bridge on Sawyer's Bar-Forks of Salmon Rd. 1.7 mi. E of Forks of Salmon. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

527 18 1 California Siskiyou 7E lON 3 Forks of Salmon-Somesbar Rd. 3.1 mi. W of Forks of Salmon Guard Sta- tion and 1, 1 mi. E of Orleans Mt. L.O. Rd. Salmon R. canyon. 528 18 2 California Siskiyou 7E 11N 21 Forks of Salmon-Somesbar Rd. 6. 9 mi. W of Forks of Salmon Guard Sta- tion and 3. 3 mi. W of Orleans Mt. L. 0. Rd. Salmon R. canyon.

529 18 1 California Siskiyou 7E 11N 17 Somesbar-Forks of Salmon Rd. 4.6 mi. E of Oak Bottom campground entrance.Banks of Salmon R.

530 18 1 California Siskiyou 6E 11N 3 0. 2 mi. E of Calif. Hwy. 96 on Somesbar-Forks of Salmon Rd.Cut bank of Salmon R. canyon.

531 18 2 California Siskiyou 6E 11N 3 0. 4 mi. S of Salmon R. bridge on Calif. Hwy. 96.Large population on banks around timber scaling station. Klamath R. valley bluffs.

532 18 1 California Humboldt 5E 10N 16 Base of E-facing bluff of Klamath R. canyon 2. 2 mi. N of Slate Creek bridge on Calif. Hwy. 96.

533 27 2 California Humboldt 4E 9N 1 Roadside cut of Calif. Hwy. 96, 0. 5 mi. S of Six Rivers Nat. For. boundary marker.

534 18 1 California Humboldt 4E 9N 3 Pecwan Rd. 0.1 mi. W of junction with Calif. Hwy. 96. S-facing bank at edge of oak-conifer woods; bluffs of op Ln Klamath R. canyon. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

535 27 1 California Humboldt 4E 9N 5 Roadside banks of Pecwan Rd. 1.4 mi. W of Calif. Hwy. 96 junction. Klamath R. bluffs. 536 27 2 California Humboldt 4E 10S 18 Pecwan Rd. 0.1 mi. E of Coon Creek bridge.S-facing cut banks with large population. With Microseris, 537 27 2 California Humboldt 3E lON 3 Scattered plants, banks of Pecwan Rd. 4.8 mi. W of Coon Creek bridge. Klamath R. bluffs. 538 27 2 California Humboldt 3E 11N 28 Large population, side of Pecwan Rd. 8.6 mi. W of Coon Creer_c bridge. 539 27 2 California Humboldt 3E 11N 17 Large population on flood plain of Klamath R. 0.3 mi. W of Pecwan Creek bridge. S40 27 3 California Humboldt 3E 9N 11 Bald Hills Rd. 7,2 mi. W of Klamath R. bridge. Very old cut-over area; large population. Top of mountains of Klamath R. canyon; French Camp Ridge, Wiregrass Prairie. 541 27 2 California Humboldt 3E 9N 22 Large population; ridge top 0.6 mi. W of junction of Hoopa Rd. with Bald Hills Rd. on latter. 542 27 2 California Humboldt 2E 9N 24 Junction of 3 dirt roads, open grassland; ridge top 5.8 mi. W of Hoopa Rd. junction on Bald Hills Rd. Williams Ridge, Childs Hill Prairie. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

543 27 2 California Humboldt 2E 10N 33 Bald Hills Rd. 0. 2 mi. W of junction with Johnson Rd. Open grassland with scattered Pseudotsuga and Quercus. 544 27 1 California Humboldt 1E 11N 25 Single plant in margin of Bald Hills Rd. 2.6 mi. E of U. S. Hwy. 101 junction in dense redwood forest. 545 18 2 California Humboldt 1E 11N 35 Cut-over redwood forest; large population roadside of Bald Hills Rd. 1.6 mi. E of U. S. Hwy. 101.

546 18 2 California Humboldt lE lON 3 Levee of Redwood Creek opposite Orick city limit sign, N-side of city. 547 18 1 California Del Norte 1E 13N 33 Large clonal clump; bank cut of U.S. Hwy. 101, 0.6 mi. N of Del Norte- Humboldt Co. line; cut-over redwood area.

548 18 2 California Del Norte 1E 13N 3 Klamath R. valley 1.0 mi. N of bridge on U, S. Hwy. 101 and 0, 5 mi. S of Requa junction.Base of bluffs. 549 18 1 California Del Norte 1E 14N 18 Forested ocean bluffs, U. S. Hwy. 101, 9.0 mi. S of Bluff Rd. S.-side of Crescent City.

550 18 2 California Del Norte 1W 15N 1 Forest bluffs, edge of Smith R. valley; U. S. Hwy. 101, 1.3 mi. S of Bluff Rd., S-side of Crescent City opposite vista point. Appendix I Continued. Accession Chromosome Number of number number n .lants counted State Count Ran e Townshi. Section Notes

551 27 2 California Del Norte 1W 16N 35 Large population, roadside and ditch; Bluff Rd. and U. S. Hwy. 101; 1.0 mi. S of Crescent City city limit sign. Smith R. valley. 552 27 2 Oregon Josephine 8W 40S 19 Old O'Brien-Takilma Rd. 1.0 mi. E of U. S. Hwy. 199.Fence row and open fields.Illinois R. valley. 553 27 2 Oregon Josephine 8W 40S 26 Happy Camp Rd. 0. 4 mi. E of junction with Takilma Rd. Fence row and open valley of Illinois R. 554 27 2 Oregon Josephine 7W 40S 32 Open woods 5. 2 mi. E of Takilma Rd. intersection on Happy Camp Rd. Scattered plants on hillsides. 555 18 2 California Siskiyou 7E 18N 29 Slag heaps of mining operation 0. 5 mi. S of bridge over West Branch Creek on Happy Camp Rd. 556 18 2 California Siskiyou 7E 17N 17 Happy Camp Rd. 4. 8 mi. W of Calif. Hwy. 96 junction. Open banks opposite abandoned lumber mills and access road to mill pond.

557 18 2 California Siskiyou 7E 16N 2 Large population in farm yard adjacent to Happy Camp Rd. 0. 4 mi. W of Calif. Hwy. 96 junction, 558 18 2 California Siskiyou 8E 17N 32 Calif. Hwy. 96, 4.6 mi. N of Happy Camp city limit sign; roadside banks. Klamath R. canyon. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

559 18 1 California Siskiyou 8E 17N 17 Banks of Thompson Creek, Klamath R. valley on Calif. Hwy. 96, 11.0 mi. E of Happy Camp city limits. 560 18 2 California Siskiyou 12W 46N 5 0. 8 mi. E of Fort Goff campground entrance on Calif. Hwy. 96.Bluffs of Klamath R. valley.

561 18 1 California Siskiyou 11W 46N 18 1. 5 mi. E of Seiad Valley Ranger Sta- tion on Calif. Hwy. 96. Klamath R. valley. 562 18 2 California Siskiyou 11W 46N 27 Gravel bar of Klamath R. adjacent to Calif. Hwy. 96, 0. 8 mi. E of O'Neil campground entrance. 563 18 2 California Siskiyou 10W 45N 6 Calif. Hwy. 96, 2.0 mi. E of Ham- burg city limits.Base of Klamath R. bluffs.

564 18 2 California Siskiyou lOW 45N 8 E-facing bluff, Scott R. canyon 1.6 mi. S of Calif. Hwy. 96 junction on Scott R. Rd. and 1, 7 mi. N of Scott Bar Ranger Station. 565 18 2 California Siskiyou lOW 45N 29 Banks of McGruffy Creek at Scott R. Rd. 2. 5 mi. S of Scott Bar Ranger Station.

566 18 2 California Siskiyou 11W 44N 2 Scott R. Rd. at Townsend Gulch; base of cut; large population.Scott R. canyon 4.0 mi. N of Kelsey Creek Ranger Station. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

567 18 2 California Siskiyou 11W 44N 21 Scott R. canyon 0.5 mi. S of Kelsey Creek Ranger Station on Scott R. Rd. Clearing in forest at road margin. 568 18 2 California Siskiyou IOW 44N 19 Scott R. Rd. 1.0 mi. E of Klamath Nat. For, boundary marker; Scott R. canyon; open pine forests.

569 18 1 California Siskiyou 9W 44N 29 Single plant in fence row; cultivated irrigated farmland, Scott R. valley 2. 5 mi. W of Fort Jones city limit sign on Scott R. Rd. 570 18 2 Oregon Multnomah 3E 1N 29 Edge of field 0. 5 mi. E of Troutdale W city limit sign on Columbia R. Scenic Hwy. and 0.6 mi. E of junction with Interstate Hwy. 80. 571 18 2 Oregon Multnomah 4E 1N 35 Columbia R. Scenic Hwy. at Little Page Rd. intersection 0. 8 mi. E of Corbett High School; bluff top of Columbia R. gorge. 572 18 2 Oregon Multnomah SE 1N 28 Columbia R. Scenic Hwy. 0. 4 mi. W of Wakeena Falls; base of Columbia R. cliffs in open areas. 573 18 2 Oregon Hood River 7E 2N 23 S-side of Interstate Hwy. 80,1. 5 mi. W of Cascade Locks exit ramp; open roadside bank.

574 18 1 Oregon Hood River 10E 3N 27 S-side of Interstate Hwy. 80 at base of Columbia R. cliffs 1.0 mi. W of Hood River west exit ramp. O Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

575 18 1 Oregon Wasco 13E 2N 17 Hillside behind rest area on Interstate Hwy. 80, 9. 5 mi. W of The Danes, Ore.

576 18 1 Oregon Wasco 13E 1N 2 Bank and ditch S-side of Interstate Hwy. 80 under "Dufur-Washington Points Next Right"; The Dalles, Ore. Between freeway and RR yards; Columbia R. valley. 577 18 1 Oregon Umatilla 27E 5N 13 Roadside of U. S. Hwy. 730, 2.0 mi. W of Umatilla city limits. Wet ditches both sides of roadway; Columbia R. valley. 578 18 2 WashingtonBenton 28E 5N 5 Large population, fence row adjacent to Wash. Hwy. 14 (12) 1.0 mi. N of Plymouth.Hills of Columbia R. valley. 579 18 1 WashingtonBenton 29E 8N 10 Large clone, bank of irrigation canal 0. 5 mi. S of Kennewick city limit sign on Wash. Hwy. 14 (12). 580 18 2 WashingtonBenton 29E 9N 29 South levee of Columbia R. adjacent to U. S. Hwy. 12 opposite Columbia Park 2. 4 mi. W of junction with Wash. Hwy. 14 (12). 581 18 2 WashingtonKlickitat 21E 3N 17 Wet bottoms behind RR embankment 0. 4 mi. E of Roosevelt train depot on Wash. Hwy. 14 (12); Columbia R. valley. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

582 18 2 WashingtonSkamania 7E 2N 14 Roadside cut of Wash. Hwy. 14 (12), 0.5 mi. W of the Bridge of the Gods Rd. junction; Columbia R. gorge. 583 18 2 WashingtonSkamania 6E 2N 25 Roadside opposite Beacon Rock State Park parking area on Wash. Hwy 14 (12). Columbia R. gorge bluffs.

584 18 1 WashingtonSkamania SE 1N 9 Roadside of Wash. Hwy. 14 (12), 3.6 mi. E of Clark-Skamania Co. line marker; Columbia R. bluffs.

585 27 1 WashingtonLewis 1W 12N 9 Roadside ditch, U. S. Hwy. 12, 0.4 mi. E of junction with Interstate Hwy. 5. 586 27 2 WashingtonLewis 2E 12N 17 Fence row, U. W. Hwy. 12, at May- field Dam Rd. junction; base of tele- phone pole.

587 27 1 WashingtonLewis 4E 12N 1 1. 3 mi. E of Wash. Hwy. 7 on U. S. Hwy. 12; roadside, opposite signpost 99; Cowlitz R. valley.

588 27 1 WashingtonLewis 8E 12N 9 Edge of woods, 50 yd. E of Davis Creek bridge on U. S. Hwy. 12, ca. mi. E of Maple Leaf campground entrance; Cowlitz R. valley.

589 27 1 WashingtonLewis 9E 13N 11 Cut-over Pseudotsuga forest0.6 mi. W of Lake Creek bridge on U. S. Hwy. 12; Cowlitz R. valley. Appendix I Continued. Accession Chromosome Number of number number n .lants counted State Ran e Townshi. Section Notes

590 27 1 WashingtonLewis 10E 14N 20 Wash. Hwy. 123, 0. 9 mi. N of junction with U. S. Hwy. 12,Roadside clearing in dense Pseudotsup forests. 591 18 1 WashingtonPierce 10E 16N 35 Roadside of Wash. Hwy. 123, 0. 2 mi. N of tunnel and 2.6 mi. S of junction with Wash. Hwy. 410.Dense coniferous forests. 592 18 1 WashingtonPierce 10E 18N 6 2.0 mi. S of Dallas Campground entrance on Wash. Hwy. 410.Isolated plant in hwy. right of way. 593 18 4 Oregon Wallowa 44E 3S 17 Achenes, ridge leading to Twin Peak above Fall Creek tributary.Elev. 7200-8500 ft.A. Legge, 594 27 2 WashingtonKing 7E 19N 12 Roadside of Wash. Hwy. 410 opposite mileage marker 31, 2, 5 mi. S of Mud Mountain Dam Rd. White R. valley. 595 18 1 WashingtonKing 6E 20N 13 Scattered plants, fence row, Wash. Hwy. 169,Suburban Enumclaw, Wash. opposite Rainier Stables O. 1 mi. S of Neawaukum Creek bridge. 596 27 1 WashingtonKing 6E 22N 27 Junction of Wash. Hwys. 516 and 169. Vacant lot, NE corner.

597 18 1 WashingtonKing 7E 25N S Roadside of Wash. Hwy, 203, O. 1 mi. N of Stillwater general store.Bluffs of Snohomish R. valley. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

598 18 2 WashingtonSnohomish 6E 27N 1 Railroad right of way to Western Farm Service at junction of Wash. Hwy. 203 and U. S. Hwy. 2; Monroe, Wash.

599 27 1 WashingtonSnohomish SE 29N 36 Junction of Wash. Hwy. 9 and U. S. Hwy. 2.Waste area of Y-intersection.

600 18 2 WashingtonSnohomish 5E 30N 29 Marsh at end of bridge on Marine Dr. 0. 7 mi. W of junction with Interstate Hwy. S.Large population on road embankment.

601 18 1, WashingtonSnohomish 4E 30N 22 Roadside of Marine Dr. opposite en- 2n = 45 1 trance to Tula lip Shores Resort 8.5 mi. W of junction with Interstate Hwy. 5.

602 18 1 WashingtonWhatcom 3E 38N 7 Undeveloped lot, corner of U. S. Hwy. 99 and Bakerview Rd. Suburban Bell- ingham, Wash.

603 18 1, WashingtonWhatcom 3E 39N 18 Roadside of U. S. Hwy. 99A ca. 1.0 2n = 45 1 mi. N of Laurel, Wash. city limits, opposite Draper Valley Chicken Hatch- ery.

604 18, 1, WashingtonWhatcom 2E 40N 13 0. 7 mi. N of Lynden, Wash, city limit 27 1 sign on U. S. Hwy. 99A, open farmland.

605 18 1 British -- -- ..... Roadside of B. C. Hwy. 13, 0. 9 mi. N Columbia of Aldergrove Canadian Customs Sta- tion; 3.1 mi. S of junction with Trans- Canada Hwy. 1. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

606 18 1 British Vancouver Island Roadside of Trans-Canada Hwy. 1 at Columbia Morden Rd. intersection, 1.6 mi. N of Nanaimo Lake Rd. junction.

607 27 1 British Vancouver Island Pink flowered clone in graveled margin Columbia of Trans-Canada Hwy. 1 at John Bo line's Ayshire Farm entrance; 30 mi. N of Victoria at mileage marker.

608 18 2 British Vancouver Island Roadside of Trans-Canada Hwy. 1, Columbia 0. 7 mi. N of Cobble Hill Rd. junction and 1.3 mi. N of Shawnigan Lake Rd. junction; large population.

609 18 1 British Vancouver Island Irrigation ditch in center of truck farm Columbia field on West Saanich Rd. 4.0 mi. NW of junction with B. C. Hwy. 17; large clone.

610 18 3 - WashingtonClallam 3W 29N 20 Palo Alto Rd. 0.1 mi. E of farm home and 1. 8 mi. S of Olympic Nat. For. boundary. Fence row in open valley, large population.

611 18 1, WashingtonClallam 3W 29N 2 Palo Alto Rd. 0.3 mi. S of junction 2n = 45 1 with U. S. Hwy. 101, roadside ditch. 612 18 1, WashingtonKitsap 1E 27N 26 Roadside of Wash. Hwy. 104, 3. 4 2n = 45 1 mi. S of Hood Canal Bridge toll gate.

613 18 2 WashingtonKitsap lE 24N 22 Wash. Hwy. 3 roadside, W-end of Bremerton Naval Yard.Waste area of Y-intersection with Charleston Beach Rd. .0 Ui Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

614 18 1 Washington Mason 1W 22N 20 S-end of Sherwood Creek bridge on Wash. Hwy. 3; Sedge of Allyn, Wash. 615 27 2 WashingtonMason 3W 21N 36 Banks of Cranberry Creek at Wash. Hwy. 3, tall plants (4. 5 ft.).

616 18 2 WashingtonMason 3W 20N 17 Drainage ditch adjacent to Wash. Hwy. 3 opposite Shelton, Wash. water treatment plant and dog pound.

617 27 1 WashingtonMason 4W 20N 18 Matlock-Shelton Rd. 300 yd. E of Dayton Community Hall at Little Egypt-Airport Rd. intersection. 618 27 1 WashingtonMason 3W 20N 12 Matlock-Shelton Rd. 8.` mi. E of Matlock general store and 1.5 mi. W of Dayton Community Hall.

619 27 1 WashingtonMason 6W 20N 15 Matlock-Satsop Rd. 1.5 mi. W, of Matlock general store; graveled margin of road.

620 27 1 WashingtonGrays Harbor 7W 20N 24 Grisdale Rd. and Matlock-Satsop Rd. intdrsection.

621 27 1 WashingtonGrays Harbor 8W 19N 11 Wynoochee R. valley Rd. 3.6 mi. S of Grisdale Rd. intersection and 8.2 mi. N of Wynoochee State Park entrance.

622 27 1 WashingtonGrays Harbor 8W 18N 4 Bluffs of Wynoochee R. on Wynoochee R. valley Rd. 0.8 mi. S of Wynoo- chee State Park entrance. Appendix I Continued. Accession Chromosome Number of number number n Cants counted S Count Ran -e Townshi. Section Notes

623 27 2 WashingtonGrays Harbor 8W 17N 2 Wynoochee R. bluf6 on Wynoochee R. valley Rd. 1. 2 mi. N of junction with U. S. Hwy. 410.

624 27 2 WashingtonGrays Harbor 7W 17N 19 Chehalis R. bluffs adjacent to Wash. Hwy. 107, 1. 7 mi. S of bridge at Melbourne, Wash.

625 18 1 WashingtonPacific 9W 10N 28 Graveled margin of Wash. Hwy. 401, 6. 8 mi. S of junction with Wash. 4.

626 18 2 WashingtonPacific 9W 9N 18 Marsh and embankment of Wash. Hwy. 401, 10. 3 mi. S of Wash. Hwy. 4 junction and 1. 8 mi. E N-end of Astoria-Columbia R. toil bridge; Columbia R. valley.

627 18, 1, WashingtonPacific 10W 9N 24 Banks of Columbia R. adjacent to 27 1 Wash. Hwy. 401, 0. 5 mi. E of N-end of Astoria-Columbia R. toll bridge-, Columbia R. valley.

628 18 2 WashingtonPacific lOW 9N 23 Bluffs of Columbia R. 100 yd. E of N-end of Astoria-Columbia R. toll bridge; large population.

629 27 2 WashingtonWahkiakum 8W lON 19 Wash. Hwy. 4 at Wainamo Rd. junction 3.0 mi. E of Salmon Creek bridge and 0. 8 mi. W of Deep R. bridge.

630 27 2 WashingtonWahkiakum 6W 9N 7 Fence row adjacent to Wash. Hwy. 4, 1.0 mi. W of Skamokawa Creek bridge. Appendix I Continued. Accession Chromosome Number of number number (n) plants counted State County Range Township Section Notes

631 27 1 WashingtonCowlitz 4W 8N 12 Bunker Hill Rd. intersection with Wash. Hwy. 4, 1. 4 mi. E of Aber- nathy Creek bridge.Columbia R. valley bluffs.

632 18 1 British Vancouver Island Near town of Campbell River.July Columbia 11, 1969. _J. H. Wales.

633 18 1 British Vancouver Island Kelsey Bay, 50 mi. N of Campbell Columbia River; Vancouver Island.July 4, 1969. J. H. Wales.

634 18 1 British Quadra Island Close to beach, 3.0 mi. N of Cape Columbia Mudges; E-side of Quadra Island. June 27, 1969. J. H. Wales.

635 18 1 British Quadra Island Gowlland Harbor, Quadra Island. Columbia June 30, 1969.J. H. Wales.