Diversity and Co-Occurrence Patterns of microbial Communities in the Fertilized Eggs of Thitarodes Insect, the Implication on the Occurrence of Chinese cordyceps Lian-Xian Guo Guangdong Medical University Xiao-Shan Liu Guangdong Medical University Zhan-Hua Mai Guangdong Medical University Yue-Hui Hong Guangdong Hospital of Traditional Chinese Medicine Qi-Jiong Zhu Guangdong Medical University Xu-Ming Guo Guangdong Medical University Huan-Wen Tang (
[email protected] ) Guangdong Medical University Research article Keywords: Bacterial, Fungal, Fertilized Eggs, Thitarodes, Chinese cordyceps, Wolbachia Posted Date: April 28th, 2020 DOI: https://doi.org/10.21203/rs.3.rs-24686/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/20 Abstract Background The large-scale articial cultivation of Chinese cordyceps has not been widely implemented because the crucial factors triggering the occurrence of Chinese cordyceps have not been fully illuminated. Methods In this study, the bacterial and fungal structure of fertilized eggs in the host Thitarodes collected from 3 sampling sites with different occurrence rates of Chinese cordyceps (Sites A, B and C: high, low and null Chinese cordyceps, respectively) were analyzed by performing 16S RNA and ITS sequencing, respectively. And the intra-kingdom and inter-kingdom network were analyzed. Results For bacterial community, totally 4671 bacterial OTUs were obtained. α-diversity analysis revealed that the evenness of the eggs from site A was signicantly higher than that of sites B and C, and the dominance index of site A was signicantly lower than that of sites B and C ( P < 0.05).