Succession in the Petroleum Reservoir Microbiome Through an Oil Field Production Lifecycle
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Coal and Oil: the Dark Monarchs of Global Energy – Understanding Supply and Extraction Patterns and Their Importance for Futur
nam et ipsa scientia potestas est List of Papers This thesis is based on the following papers, which are referred to in the text by their Roman numerals. I Höök, M., Aleklett, K. (2008) A decline rate study of Norwe- gian oil production. Energy Policy, 36(11):4262–4271 II Höök, M., Söderbergh, B., Jakobsson, K., Aleklett, K. (2009) The evolution of giant oil field production behaviour. Natural Resources Research, 18(1):39–56 III Höök, M., Hirsch, R., Aleklett, K. (2009) Giant oil field decline rates and their influence on world oil production. Energy Pol- icy, 37(6):2262–2272 IV Jakobsson, K., Söderbergh, B., Höök, M., Aleklett, K. (2009) How reasonable are oil production scenarios from public agen- cies? Energy Policy, 37(11):4809–4818 V Höök M, Söderbergh, B., Aleklett, K. (2009) Future Danish oil and gas export. Energy, 34(11):1826–1834 VI Aleklett K., Höök, M., Jakobsson, K., Lardelli, M., Snowden, S., Söderbergh, B. (2010) The Peak of the Oil Age - analyzing the world oil production Reference Scenario in World Energy Outlook 2008. Energy Policy, 38(3):1398–1414 VII Höök M, Tang, X., Pang, X., Aleklett K. (2010) Development journey and outlook for the Chinese giant oilfields. Petroleum Development and Exploration, 37(2):237–249 VIII Höök, M., Aleklett, K. (2009) Historical trends in American coal production and a possible future outlook. International Journal of Coal Geology, 78(3):201–216 IX Höök, M., Aleklett, K. (2010) Trends in U.S. recoverable coal supply estimates and future production outlooks. Natural Re- sources Research, 19(3):189–208 X Höök, M., Zittel, W., Schindler, J., Aleklett, K. -
Norwegian Petroleum Technology a Success Story ISBN 82-7719-051-4 Printing: 2005
Norwegian Academy of Technological Sciences Offshore Media Group Norwegian Petroleum Technology A success story ISBN 82-7719-051-4 Printing: 2005 Publisher: Norwegian Academy of Technological Sciences (NTVA) in co-operation with Offshore Media Group and INTSOK. Editor: Helge Keilen Journalists: Åse Pauline Thirud Stein Arve Tjelta Webproducer: Erlend Keilen Graphic production: Merkur-Trykk AS Norwegian Academy of Technological Sciences (NTVA) is an independent academy. The objectives of the academy are to: – promote research, education and development within the technological and natural sciences – stimulate international co-operation within the fields of technology and related fields – promote understanding of technology and natural sciences among authorities and the public to the benefit of the Norwegian society and industrial progress in Norway. Offshore Media Group (OMG) is an independent publishing house specialising in oil and energy. OMG was established in 1982 and publishes the magazine Offshore & Energy, two daily news services (www.offshore.no and www.oilport.net) and arranges several petro- leum and energy based conferences. The entire content of this book can be downloaded from www.oilport.net. No part of this publication may be reproduced in any form, in electronic retrieval systems or otherwise, without the prior written permission of the publisher. Publisher address: NTVA Lerchendahl gaard, NO-7491 TRONDHEIM, Norway. Tel: + (47) 73595463 Fax: + (47) 73590830 e-mail: [email protected] Front page illustration: FMC Technologies. Preface In many ways, the Norwegian petroleum industry is an eco- passing $ 160 billion, and political leaders in resource rich nomic and technological fairy tale. In the course of a little oil countries are looking to Norway for inspiration and more than 30 years Norway has developed a petroleum guidance. -
Structure and Function of Benzylsuccinate Synthase and Related Fumarate-Adding Glycyl Radical Enzymes
Review Article J Mol Microbiol Biotechnol 2016;26:29–44 Published online: March 10, 2016 DOI: 10.1159/000441656 Structure and Function of Benzylsuccinate Synthase and Related Fumarate-Adding Glycyl Radical Enzymes a d c a Johann Heider Maciej Szaleniec Berta M. Martins Deniz Seyhan a, b e Wolfgang Buckel Bernard T. Golding a Laboratory of Microbial Biochemistry, LOEWE Center for Synthetic Microbiology, Philipps University Marburg, b c and Max-Planck-Institut für terrestrische Mikrobiologie, Marburg , and Institut für Biologie, Strukturbiologie/ d Biochemie, Humboldt-Universität zu Berlin, Berlin , Germany; Jerzy Haber Institute of Catalysis and Surface e Chemistry, Polish Academy of Sciences, Kraków , Poland; School of Chemistry, Newcastle University, Newcastle upon Tyne , UK Key Words Anaerobic Toluene Degradation Benzylsuccinate synthase · Fumarate-adding enzyme · Glycyl radical · Toluene · Alkane · Anaerobic toluene Hydrocarbons were long believed to be resistant to degradation microbial degradation in the absence of oxygen. It was therefore surprising to find that many bacteria degrade these compounds anaerobically [Aeckersberg et al., 1991; Abstract Dolfing et al., 1990; Rabus et al., 1993; Vogel and Grbic- The pathway of anaerobic toluene degradation is initiated Galic, 1986; Zeyer et al., 1986]. After the initial reports by a remarkable radical-type enantiospecific addition of the dating from 1986, the degradation pathways employed chemically inert methyl group to the double bond of a fuma- by these bacteria remained enigmatic for a decade. How- rate cosubstrate to yield (R) -benzylsuccinate as the first in- ever, over the last 20 years, various oxygen-independent termediate, as catalyzed by the glycyl radical enzyme ben- reactions have been characterized for the activation of zylsuccinate synthase. -
Abstract Gas-Phase Chemistry of Tryptophan
ABSTRACT GAS-PHASE CHEMISTRY OF TRYPTOPHAN-BASED RADICALS Andrii Piatkivskyi, Ph.D. Department of Chemistry and Biochemistry Northern Illinois University, 2014 Victor Ryzhov, Director This work is devoted to the fundamental study of gas-phase tryptophan-based radical cations. It covers: mechanisms of the radical ion formation in the gas-phase; the description and contrast of the two types of tryptophan side chain radicals (π and N-indolyl) based on their reactivities, infrared spectra, structural energetics and fragmentation patterns; investigation of the N-indolyl tryptophan radical cation fragmentation pathways; formation of the two types of tryptophan radicals (π and N-indolyl) within short peptides; their characterization and comparison based on the fragmentation patterns and reactivity; and gas-phase study of intramolecular radical migration from tryptophan to cysteine and from tryptophan to tyrosine side chains within short peptides. Two different approaches were followed to regiospecifically form desired radicals on the tryptophan side chain. The tryptophan π-radical cation was formed via electron transfer during dissociation of ternary metal complex ([CuII(terpy)(Trp)] 2+), while N-indolyl radical was formed by the homolytic cleavage of the NO group from the N-nitrosylated tryptophan during gas-phase fragmentation. Both radicals were exposed to the low-energy collision-induced dissociation in order to elucidate and contrast their fragmentation. To estimate the fragmentation pathway of the N-indolyl tryptophan radical cation, additional experiments (including H/D exchange, dissociation of tryptophan derivatives and DFT calculations) were carried out. The radical reactivity has been tested via gas-phase ion-molecule reactions with benzeneselenol, 1-propanethiol and di-tert-butyl nitroxide. -
Annual Report 2019 the 2019 Partners
THE NATIONAL IOR CENTRE OF NORWAY Annual report 2019 The 2019 partners ConocoPhillips Observers Contents Management Team 4 Board Members 4 Technical Committee 5 Scientific Advisory Committee 5 About 6 Research Themes 7 Greetings from the Director 8 Greetings from the Chairman of the Board 9 Consortium Partners 10 Activity Highlights On the use of thermo-thickening polymers for in-depth mobility control – large scale testing 12 Developing the ensemble Kalman filter based approach for history matching 15 Coordinating Upscaling Workflow 16 Interpore – 3rd National Workshop on Porous Media 17 New management team 18 Great expectations from the Research Council of Norway 19 Researcher Profiles Mehul Vora 20 André Luís Morosov 21 Micheal Oguntola 21 Tine Vigdel Bredal 22 Arun Selvam 22 Panagiotis Aslanidis 23 William Chalub Cruz 23 Nisar Ahmed 24 Aleksandr Mamonov 24 Juan Michael Sargado 25 Runar Berge 25 Highlights from the PhD Defences PhD Defences 2019 26 Core scale modelling of EOR transport mechanisms 27 Smart Water for EOR from Seawater and Produced Water by Membranes 27 Wettability estimation by oil adsorption 28 CO2 Mobility Control with Foam for EOR and Associated Storage 28 Interaction between two calcite surfaces in aqueous solutions … 29 Research Dissemination, Conferences & Awards Lundin Norway shares all data from one field 30 PhD student Mehul Vora presented at OG21 31 Selected Papers 32 Media Contributions 2019 35 Who are we? 36 Publications 37 3 Management Team Ying Guo Tina Puntervold Randi Valestrand Centre Director Assistant -
Constraint-Based Modeling Analysis of the Metabolism of Two Pelobacter Species Jun Sun1*, Shelley a Haveman2,3, Olivia Bui1, Tom R Fahland1,4, Derek R Lovley2
Sun et al. BMC Systems Biology 2010, 4:174 http://www.biomedcentral.com/1752-0509/4/174 RESEARCH ARTICLE Open Access Constraint-based modeling analysis of the metabolism of two Pelobacter species Jun Sun1*, Shelley A Haveman2,3, Olivia Bui1, Tom R Fahland1,4, Derek R Lovley2 Abstract Background: Pelobacter species are commonly found in a number of subsurface environments, and are unique members of the Geobacteraceae family. They are phylogenetically intertwined with both Geobacter and Desulfuromonas species. Pelobacter species likely play important roles in the fermentative degradation of unusual organic matters and syntrophic metabolism in the natural environments, and are of interest for applications in bioremediation and microbial fuel cells. Results: In order to better understand the physiology of Pelobacter species, genome-scale metabolic models for Pelobacter carbinolicus and Pelobacter propionicus were developed. Model development was greatly aided by the availability of models of the closely related Geobacter sulfurreducens and G. metallireducens. The reconstructed P. carbinolicus model contains 741 genes and 708 reactions, whereas the reconstructed P. propionicus model contains 661 genes and 650 reactions. A total of 470 reactions are shared among the two Pelobacter models and the two Geobacter models. The different reactions between the Pelobacter and Geobacter models reflect some unique metabolic capabilities such as fermentative growth for both Pelobacter species. The reconstructed Pelobacter models were validated by simulating published growth conditions including fermentations, hydrogen production in syntrophic co-culture conditions, hydrogen utilization, and Fe(III) reduction. Simulation results matched well with experimental data and indicated the accuracy of the models. Conclusions: We have developed genome-scale metabolic models of P. -
Metagenomes of Native and Electrode-Enriched Microbial Communities from the Soudan Iron Mine Jonathan P
Metagenomes of native and electrode-enriched microbial communities from the Soudan Iron Mine Jonathan P. Badalamenti and Daniel R. Bond Department of Microbiology and BioTechnology Institute, University of Minnesota - Twin Cities, Saint Paul, Minnesota, USA Twitter: @JonBadalamenti @wanderingbond Summary Approach - compare metagenomes from native and electrode-enriched deep subsurface microbial communities 30 Despite apparent carbon limitation, anoxic deep subsurface brines at the Soudan ) enriched 2 Underground Iron Mine harbor active microbial communities . To characterize these 20 A/cm µ assemblages, we performed shotgun metagenomics of native and enriched samples. enrich ( harvest cells collect inoculate +0.24 V extract 10 Follwing enrichment on poised electrodes and long read sequencing, we recovered Soudan brine electrode 20° C from DNA biodreactors current electrodes from the metagenome the closed, circular genome of a novel Desulfuromonas sp. 0 0 10 20 30 40 filtrate PacBio RS II Illumina HiSeq with remarkable genomic features that were not fully resolved by short read assem- extract time (d) long reads short reads TFF DNA unenriched bly alone. This organism was essentially absent in unenriched Soudan communities, 0.1 µm retentate assembled metagenomes reconstruct long read return de novo complete genome(s) assembly indicating that electrodes are highly selective for putative metal reducers. Native HGAP assembly community metagenomes suggest that carbon cycling is driven by methyl-C me- IDBA_UD 1 hybrid tabolism, in particular methylotrophic methanogenesis. Our results highlight the 3 µm prefilter assembly N4 binning promising potential for long reads in metagenomic surveys of low-diversity environ- borehole N4 binning read trimming and filtering brine de novo ments. -
26Sor Em2rray /Mmucd
D4006- Gut Microbiota Analysis UC Davis MMPC - Microbiome & Host Response Core Contents 1 Methods: 1 1.1 Sequencing . .1 1.2 Data processing . .1 2 Summary of Findings: 2 2.1 Sequencing analysis . .2 2.2 Microbial diversity . .2 2.2.1 Alpha Diversity . .2 2.2.2 Beta Diversity . 10 2.3 Data analysis using taxa abundance data . 13 2.3.1 Stacked bar graphs of Taxa abundances at each level . 14 A Appendix 1 (Taxa Abundance Tables) 30 B Appendix 2 (Taxa removed from filtered datasets at each level) 37 References: 43 Core Contacts: Helen E. Raybould, Ph.D., Core Leader ([email protected]) Trina A. Knotts, Ph.D., Core Co-Leader ([email protected]) Michael L. Goodson, Ph.D., Core Scientist ([email protected]) Client(s): Kent Lloyd, DVM PhD ;MMRRC; UC Davis Project #: MBP-2079 MMRRC strain ID: MMRRC_043514 Animal Information: The strain was donated to the MMRRC by Russell Ray at Baylor College of Medicine. Fecal samples were obtained from animals housed under the care of Russell Ray at Baylor College of Medicine. 1 Methods: Brief Project Description: MMRRC strains are often contributed to the MMRRC to fulfill the resource sharing aspects of NIH grants. Since transporting mice to another facilty often causes a microbiota shift, having a record of the original fecal microbiota from the donor institution where the original phenotyping or testing was performed may prove helpful if a phenotype is lost after transfer. Several MMRRC mouse lines were selected for fecal microbiota profiling of the microbiota. Table 1: Animal-Strain Information X.SampleID TreatmentGroup Animal_ID Genotype Line Sex MMRRC.043514.Mx054 MMRRC.043514_Het_M Mx054 Het MMRRC.043514 M MMRRC.043514.Mx415 MMRRC.043514_Het_M Mx415 Het MMRRC.043514 M 1 1.1 Sequencing Frozen fecal or regional gut samples were shipped on dry ice to UC Davis MMPC and Host Microbe Systems Biology Core. -
Petroleum Production and Exploration
To my family List of Papers This thesis is based on the following papers, which are referred to in the text by their Roman numerals. I Jakobsson, K., Söderbergh, B., Höök, M., Aleklett, K. (2009) How reasonable are oil production scenarios from public agen- cies? Energy Policy, 37(11):4809-4818 II Jakobsson, K., Bentley, R., Söderbergh, B., Aleklett, K. (2011) The end of cheap oil. Bottom-up economic and geologic model- ing of aggregate oil production curves. Energy Policy, in press III Jakobsson, K., Söderbergh, B., Snowden, S., Aleklett, K. (2011) Bottom-up modeling of oil production. Review and sensitivity analysis. Manuscript. IV Jakobsson, K., Söderbergh, B., Snowden, S., Li, C.-Z., Aleklett, K. (2011) Oil exploration and perceptions of scarcity. The falla- cy of early success. Energy Economics, in press V Söderbergh, B., Jakobsson, K., Aleklett, K. (2009) European energy security. The future of Norwegian natural gas produc- tion. Energy Policy, 37(12):5037-5055 VI Söderbergh, B., Jakobsson, K., Aleklett, K. (2010) European energy security. An analysis of future Russian natural gas pro- duction and exports. Energy Policy, 38(12):7827-7843 VII Höök, M., Söderbergh, B., Jakobsson, K., Aleklett, K. (2009) The evolution of giant oil field production behavior. Natural Resources Research, 18(1):39-56 Reprints were made with permission from the respective publishers. The author’s contribution to the papers: Papers I-IV: principal authorship and major contribution to model- ing Papers V and VI: contribution to modeling Paper VII: minor contribution to modeling Complementary paper not included in the thesis: Aleklett, K., Höök, M., Jakobsson, K., Lardelli, M., Snowden, S., Söder- bergh, B. -
Development of UASB-DHS System for Treating Industrial Wastewater Containing Ethylene Glycol
Journal of Water and Environment Technology, Vol.13, No.2, 2015 Development of UASB-DHS System for Treating Industrial Wastewater Containing Ethylene Glycol Takahiro WATARI 1), Daisuke TANIKAWA2), Kyohei KURODA1), Akinobu NAKAMURA1), Nanako FUJII3), Fuminori YONEYAMA3), Osamu WAKISAKA3), Masashi HATAMOTO1), Takashi YAMAGUCHI 1) 1) Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan 2) Department of Civil and Environmental Engineering, National Institute of Technology, Kure College, 2-1-11 Agaminami, Kure, Hiroshima 737-8506, Japan 3) Fundamental Material Laboratory Office, Material Technology Research and Development Laboratory, Research and Development Headquarters, Sumitomo Riko Company Limited, 3-1 Higashi, Komaki, Aichi 485-8550, Japan ABSTRACT This study evaluated the performance of a novel treatment system consisting of an upflow anaerobic sludge blanket (UASB) and a downflow hanging sponge (DHS) for the treatment of industrial wastewater containing 8% ethylene glycol and 2% propylene glycol discharged from a rubber production unit. The system achieved high COD removal (91 ± 4.3%) and methane recovery (82 ± 20%) at an organic loading rate of 8.5 kg-COD/(m3·day). The UASB allowed an organic loading rate of 14 kg-COD/(m3·day) with a constant hydraulic retention time of 24 h. The COD of DHS effluent was 370 ± 250 mg-COD/L during the entire experimental period. Thus, the proposed system could be applicable for treating industrial wastewater containing ethylene glycol. Massively parallel 16S rRNA gene sequencing elucidated the microbial community structure of the UASB. The dominant family Pelobacteriaceae could mainly degrade the organic compounds of ethylene glycol and decomposed products of ethanol. -
Benzylsuccinate Synthase of Azoarcus Sp. Strain T: Cloning, Sequencing, Transcriptional Organization, and Its Role in Anaerobic Toluene and M-Xylene Mineralization
JOURNAL OF BACTERIOLOGY, Dec. 2001, p. 6763–6770 Vol. 183, No. 23 0021-9193/01/$04.00ϩ0 DOI: 10.1128/JB.183.23.6763–6770.2001 Copyright © 2001, American Society for Microbiology. All Rights Reserved. Benzylsuccinate Synthase of Azoarcus sp. Strain T: Cloning, Sequencing, Transcriptional Organization, and Its Role in Anaerobic Toluene and m-Xylene Mineralization 1 1 1,2 GYPSY R. ACHONG, ANA M. RODRIGUEZ, AND ALFRED M. SPORMANN * Environmental Engineering and Science, Department of Civil and Environmental Engineering,1 and Department of Biological Sciences,2 Stanford University, Stanford, California 94305-4020 Received 18 May 2001/Accepted 23 August 2001 Biochemical studies in Azoarcus sp. strain T have demonstrated that anaerobic oxidation of both toluene and m-xylene is initiated by addition of the aromatic hydrocarbon to fumarate, forming benzylsuccinate and 3-methyl benzylsuccinate, respectively. Partially purified benzylsuccinate synthase was previously shown to catalyze both of these addition reactions. In this study, we identified and sequenced the genes encoding benzylsuccinate synthase from Azoarcus sp. strain T and examined the role of this enzyme in both anaerobic toluene and m-xylene mineralization. Based on reverse transcription-PCR experiments and transcriptional start site mapping, we found that the structural genes encoding benzylsuccinate synthase, bssCAB, together with two additional genes, bssD and bssE, were organized in an operon in the order bssDCABE. bssD is believed to encode an activating enzyme, similar in function to pyruvate formate-lyase activase. bssE shows homology Downloaded from to tutH from Thauera aromatica strain T1, whose function is currently unknown. A second operon that is upstream of bssDCABE and divergently transcribed contains two genes, tdiS and tdiR. -
Evaluating Some Aspects of Gas Geochemistry of Some North Sea Oil Fields
ienc Sc es al J c o i u m r e n a Nwaogu, Chem Sci J 2015, 6:2 h l C Chemical Sciences Journal DOI: 10.4172/2150-3494.100093 ISSN: 2150-3494 Research Article Article OpenOpen Access Access Evaluating Some Aspects of Gas Geochemistry of some North Sea Oil Fields Chukwudi Nwaogu1*,Selegha Abrakasa2, Hycienth Nwankwoala2, Mmaduabuchi Uzoegbu2 and John Nwogu3 1Faculty of Environmental Sciences, Czech University of Life Sciences, Kamycka 129, 165 21 Suchdol, Praque 6, Czech Republic. 2Department of Geology, University of Port Harcourt, Rivers State. Nigeria. 3Rivers State College of Health Technology, Port-Harcourt, Rivers State, Nigeria. Abstract Gas geochemistry, an integral part of petroleum geosciences, has been used for evaluating source rocks potential for shale gas and in conventional exploration as a guide for determining potential productive formations. However, in contemporary times, new concepts of gas geochemistry have been applied in delineating the effectiveness of caprocks. Caprock is a vital element of a petroleum system, the volumes of rocks overlaying reservoirs are responsible for the configuration on which the accumulation sits and fosters essential preservation. In this study headspace gas served the purpose of delineating leakage and determining migration pathways, mechanism and discriminating gas types in the formations overlaying the reservoirs. Keywords: Gas geochemistry; Caprocks; Leakage; Thermogenic gas; lithology of the formations were modelled using Genesis 4.8. Thermogenic signature Methodology Introduction This study is part of a wider study on regional leakage evaluation of Gas geochemistry – a new comer in the series of geochemistry has the North Sea. Data for this study were sourced from well completion proved vital in delineating caprock leakage employing headspace gas.