Metabolomics - Measuring Metabolic Adaptation
MERIT Skill Training 3 11. – 17.3.2013
Organizers
Thomas Nägele, Ella Nukarinen and Wolfram Weckwerth Department Molecular Systems Biology University of Vienna Althanstr. 14 1090 Vienna, Austria
Contact information:
Tel. Office: 0043-1-4277-57702
Mobile (Thomas): 0043-680-4444590
Summary
The systematic description of living systems requires a substantial sample throughput in parallel with comprehensive and accurate analysis of as many constituents as possible. Metabolomics is currently one of the most promising techniques for such high-throughput analyses of biological systems. As a unique diagnostic technique to confirm rapid changes in metabolism it provides insights into gene function and plant metabolism. Metabolomics technology has nowadays a broad application range from plant sciences to biotechnology and microbiology to biomedical research. For metabolomics, GC/MS has evolved as an important technology. This workshop will provide insights into basic definitions of metabolomics technology, the various techniques used, and hands-on experience in the analysis of complex metabolomic samples with state-of-the-art GC/MS instruments, data mining strategies, statistics and biological interpretation. The attendees will analyze samples from a ‘day/night/extended night experiment’ on various bZip and SnRK mutant lines.
Accomodation
Hotel Alexander Wien Augasse 15, 1090 Wien T: +43 1 317 15 08 F: +43 1 317 15 08 82 www.alexander.co.at
1 How to Arrive & Transportation in Vienna
Arrival by Plane
The Vienna International Airport (http://www.viennaairport.com) is connected via S-Bahn (http://www.schnellbahn-wien.at/), the City Airport Train (http://www.cityairporttrain.com/) and the Airport Bus (http://www.postbus.at/de/Flughafenbus/Vienna_AirportLines/) to Vienna city center. The most convenient way is to take the S-Bahn S7 in direction of Wien Floridsdorf. Get out of the train at the station Landstraße Bahnhof Wien Mitte. Then take the metro line U4 in direction of Heiligenstadt and get out at the station Spittelau. Leave the metro station to the left and follow the plan to the hotel:
http://maps.google.de/maps?saddr=Unbekannte+Stra%C3%9Fe&daddr=wien+augasse+15&hl=de&ie=UTF8&ll=48.233121,16.359394&spn=0. 008347,0.01929&sll=48.228475,16.355209&sspn=0.016696,0.038581&geocode=FVoD4AIdU535AA%3BFaDz3wId3JX5ACkpXNZlywdtRzEgAs 0utOlbqA&dirflg=w&mra=dme&mrsp=0&sz=15&t=m&z=16
Arrival by Train
You arrive at Wien Westbahnhof. Change to the metro line U6 in direction of Floridsdorf and get out at the station Spittelau. Follow the plan to the hotel as described above.
Arrival by Car
Don’t do that – go back to “Arrival by Plane” or Arrival by Train”.
Otherwise, please take a look at Google Maps.
2 Program
Sunday, 10.03.2013
Time Description Details
Arrival and Registration Hotel Alexander Wien Augasse 15, 1090 Vienna
Monday, 11.03.2013
Time Description Details
09:00 Welcome and Introduction Department Molecular Systems Biology Althanstraße 14, 1090 Vienna
09:45 Coffee break
10:00 Extraction of samples
12:00 Lunch
14:00 Continue Extraction (if necessary)
15:00 Presentation: Techniques in Metabolomics
16:30 Coffee break
17:00 Presentation: Introduction to GC-MS
19:00 Dinner
Tuesday, 12.03.2013
Time Description Details
09:00 Building of 2 groups Group 1: GC-MS Introduction
Group 2: Derivatisation/Measurement
10:30 Coffee break
11:00 Continue with GC-MS Introduction and Derivatisation
12:00 Lunch
3 14:00 GC-MS Introduction/Derivatisation Group 1: Derivatisation/Measurement
Group 2: GC-MS Introduction
16:00 Coffee break
17:00 Summary/Discussion/Questions
19:00 Dinner & Socializing
Wednesday, 13.03.2013
Time Description Details
09:00 Introduction to Data evaluation Xcalibur/Statistics…
10:30 Coffee break
11:00 Continue introduction to data evaluation
12:00 Lunch
14:00 Data evaluation of measured samples
… Coffee break
19:00 Dinner & Socializing
Thursday, 14.03.2013
Time Description Details
09:00 Data evaluation Sample extraction/derivatisation/measurement
… Coffee break
12:00 Lunch
14:00 Data evaluation Sample extraction/derivatisation/measurement
15:30 Coffee break
4 16:00 Presentation: Introduction to statistics and modelling
17:00 Data preparation for statistics and modelling
19:00 Dinner
Friday, 15.03.2013
Time Description Details
09:00 Data evaluation/statistics/modelling
10:30 Coffee break
11:00 Statistics/modelling
12:00 Lunch
14:00 Statistics/Modelling
15:30 Coffee break
16:00 Statistics/Modelling
Summary of results for short presentation
19:00 Dinner & Socializing
Saturday, 16.03.2013
Time Description Details
09:00 Summary of results for short presentation
10:00 Presentation of Results & Final Discussion
12:30 Lunch
End of the Course/Departure
5