Metabolomics - Measuring Metabolic Adaptation MERIT Skill Training 3 11. – 17.3.2013 Organizers Thomas Nägele, Ella Nukarinen and Wolfram Weckwerth Department Molecular Systems Biology University of Vienna Althanstr. 14 1090 Vienna, Austria Contact information: Tel. Office: 0043-1-4277-57702 Mobile (Thomas): 0043-680-4444590 Summary The systematic description of living systems requires a substantial sample throughput in parallel with comprehensive and accurate analysis of as many constituents as possible. Metabolomics is currently one of the most promising techniques for such high-throughput analyses of biological systems. As a unique diagnostic technique to confirm rapid changes in metabolism it provides insights into gene function and plant metabolism. Metabolomics technology has nowadays a broad application range from plant sciences to biotechnology and microbiology to biomedical research. For metabolomics, GC/MS has evolved as an important technology. This workshop will provide insights into basic definitions of metabolomics technology, the various techniques used, and hands-on experience in the analysis of complex metabolomic samples with state-of-the-art GC/MS instruments, data mining strategies, statistics and biological interpretation. The attendees will analyze samples from a ‘day/night/extended night experiment’ on various bZip and SnRK mutant lines. Accomodation Hotel Alexander Wien Augasse 15, 1090 Wien T: +43 1 317 15 08 F: +43 1 317 15 08 82 www.alexander.co.at 1 How to Arrive & Transportation in Vienna Arrival by Plane The Vienna International Airport (http://www.viennaairport.com) is connected via S-Bahn (http://www.schnellbahn-wien.at/), the City Airport Train (http://www.cityairporttrain.com/) and the Airport Bus (http://www.postbus.at/de/Flughafenbus/Vienna_AirportLines/) to Vienna city center. The most convenient way is to take the S-Bahn S7 in direction of Wien Floridsdorf. Get out of the train at the station Landstraße Bahnhof Wien Mitte. Then take the metro line U4 in direction of Heiligenstadt and get out at the station Spittelau. Leave the metro station to the left and follow the plan to the hotel: http://maps.google.de/maps?saddr=Unbekannte+Stra%C3%9Fe&daddr=wien+augasse+15&hl=de&ie=UTF8&ll=48.233121,16.359394&spn=0. 008347,0.01929&sll=48.228475,16.355209&sspn=0.016696,0.038581&geocode=FVoD4AIdU535AA%3BFaDz3wId3JX5ACkpXNZlywdtRzEgAs 0utOlbqA&dirflg=w&mra=dme&mrsp=0&sz=15&t=m&z=16 Arrival by Train You arrive at Wien Westbahnhof. Change to the metro line U6 in direction of Floridsdorf and get out at the station Spittelau. Follow the plan to the hotel as described above. Arrival by Car Don’t do that – go back to “Arrival by Plane” or Arrival by Train”. Otherwise, please take a look at Google Maps. 2 Program Sunday, 10.03.2013 Time Description Details Arrival and Registration Hotel Alexander Wien Augasse 15, 1090 Vienna Monday, 11.03.2013 Time Description Details 09:00 Welcome and Introduction Department Molecular Systems Biology Althanstraße 14, 1090 Vienna 09:45 Coffee break 10:00 Extraction of samples 12:00 Lunch 14:00 Continue Extraction (if necessary) 15:00 Presentation: Techniques in Metabolomics 16:30 Coffee break 17:00 Presentation: Introduction to GC-MS 19:00 Dinner Tuesday, 12.03.2013 Time Description Details 09:00 Building of 2 groups Group 1: GC-MS Introduction Group 2: Derivatisation/Measurement 10:30 Coffee break 11:00 Continue with GC-MS Introduction and Derivatisation 12:00 Lunch 3 14:00 GC-MS Introduction/Derivatisation Group 1: Derivatisation/Measurement Group 2: GC-MS Introduction 16:00 Coffee break 17:00 Summary/Discussion/Questions 19:00 Dinner & Socializing Wednesday, 13.03.2013 Time Description Details 09:00 Introduction to Data evaluation Xcalibur/Statistics… 10:30 Coffee break 11:00 Continue introduction to data evaluation 12:00 Lunch 14:00 Data evaluation of measured samples … Coffee break 19:00 Dinner & Socializing Thursday, 14.03.2013 Time Description Details 09:00 Data evaluation Sample extraction/derivatisation/measurement … Coffee break 12:00 Lunch 14:00 Data evaluation Sample extraction/derivatisation/measurement 15:30 Coffee break 4 16:00 Presentation: Introduction to statistics and modelling 17:00 Data preparation for statistics and modelling 19:00 Dinner Friday, 15.03.2013 Time Description Details 09:00 Data evaluation/statistics/modelling 10:30 Coffee break 11:00 Statistics/modelling 12:00 Lunch 14:00 Statistics/Modelling 15:30 Coffee break 16:00 Statistics/Modelling Summary of results for short presentation 19:00 Dinner & Socializing Saturday, 16.03.2013 Time Description Details 09:00 Summary of results for short presentation 10:00 Presentation of Results & Final Discussion 12:30 Lunch End of the Course/Departure 5 .