DOCSLIB.ORG

Effect of Diatom Based Biofilms on Paracentrotus Lividus Competent Larvae Marta Castilla-Gavilán, Meshi Reznicov, Vincent Turpin, Priscilla Decottignies, Bruno Cognie

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Effect of Diatom Based Biofilms on Paracentrotus Lividus Competent Larvae Marta Castilla-Gavilán, Meshi Reznicov, Vincent Turpin, Priscilla Decottignies, Bruno Cognie Journal Pre-proof Sea urchin recruitment: Effect of diatom based biofilms on Paracentrotus lividus competent larvae Marta Castilla-Gavilán, Meshi Reznicov, Vincent Turpin, Priscilla Decottignies, Bruno Cognie To cite this version: Marta Castilla-Gavilán, Meshi Reznicov, Vincent Turpin, Priscilla Decottignies, Bruno Cognie. Jour- nal Pre-proof Sea urchin recruitment: Effect of diatom based biofilms on Paracentrotus lividus com- petent larvae. Aquaculture, Elsevier, 2020, 515, pp.734559. 10.1016/j.aquaculture.2019.734559. hal-02378591 HAL Id: hal-02378591 https://hal.archives-ouvertes.fr/hal-02378591 Submitted on 25 Nov 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Journal Pre-proof Sea urchin recruitment: Effect of diatom based biofilms on Paracentrotus lividus competent larvae Marta Castilla-Gavilán, Meshi Reznicov, Vincent Turpin, Priscilla Decottignies, Bruno Cognie PII: S0044-8486(19)31658-8 DOI: https://doi.org/10.1016/j.aquaculture.2019.734559 Reference: AQUA 734559 To appear in: Aquaculture Received Date: 5 July 2019 Revised Date: 13 September 2019 Accepted Date: 1 October 2019 Please cite this article as: Castilla-Gavilán, M., Reznicov, M., Turpin, V., Decottignies, P., Cognie, B., Sea urchin recruitment: Effect of diatom based biofilms on Paracentrotus lividus competent larvae, Aquaculture (2019), doi: https://doi.org/10.1016/j.aquaculture.2019.734559. This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2019 Published by Elsevier B.V. 1 Sea urchin recruitment: effect of diatom based biofilms on Paracentrotus lividus 2 competent larvae 3 Marta Castilla-Gavilán*┼, Meshi Reznicov ┼, Vincent Turpin, Priscilla Decottignies, 4 Bruno Cognie 5 Université de Nantes, Institut Universitaire Mer et Littoral, EA 2160 Mer Molécules 6 Santé, 2 rue de la Houssinière BP 92208, 44322 Nantes cedex 3 (France) 7 ┼ These authors contributed equally 8 *Corresponding author: [email protected] 9 Abstract 10 Eight different experimental substrates were tested on Paracentrotus lividus competent 11 larvae in order to evaluate their potential for inducing metamorphosis and enhance 12 survival after recruitment. Two benthic diatoms species, Nitzschia laevis (NL) and 13 Halamphora coffeaeformis (HC), were selected according to their capacity to adhere 14 and to form strong biofilms. They were tested in monocultures and in a mixed biofilm 15 (MIX) that was also tried in combination with Gamma-Aminobutyric Acid, involved in 16 triggering some invertebrate metamorphosis (MIX+GABA). Histamine (HIS) was also 17 used as a treatment according to the high metamorphosis rates that have been recorded 18 for this compound on other sea urchin species. Finally, a natural microphytobenthic 19 biofilm (NATURAL) and oyster shells particles colonized by epiphytic diatoms 20 (SHELL) were sampled from the mud of a refining oyster pond. Batches of 21 days-old 21 larvae were placed on each experimental substrate and their effect was compared to a 22 negative control of filtered sea water (without any treatment; FSW). Metamorphosis rate 23 was daily recorded in each treatment. The sea urchin larvae on substrates NL, 24 NATURAL, GABA+MIX and SHELL showed significantly higher metamorphosis 25 rates than larvae on the other treatments ( P < 0.001), reaching more than 90% in 72h. -1- 26 Survival rate was assessed at 10 days post-metamorphosis in these four treatments. No 27 difference was observed between them in terms of metamorphosis rate or survival rate 28 (more than 60% for the four experimental substrates). Results demonstrate that the 29 transition from planktonic larvae to benthic juvenile could be promoted through diatom- 30 based biofilms. These substrates represent efficient metamorphosis inducers for P. 31 lividus larviculture but we suggest to use preferably N. laevis biofilms in order to 32 promote practical and safe solutions for farmers. 33 Keywords: aquaculture diversification; echinoculture; Nitzschia laevis ; metamorphosis 34 rate; post-settlement survival rate 35 Introduction 36 Sea urchin roes are considered as a delicacy and they are among the most valued sea 37 food products. Sea urchin become highly trendy due to their unique taste and the spread 38 of Japanese food around the globe. The leading country in consumption of sea urchins is 39 Japan followed by France, the first market in Europe (Stefánsson et al., 2017). To fulfil 40 this demand, wild populations have been overexploited worldwide leading to a decline 41 since the 90’s (Ceccherelli et al., 2011; Couvray et al., 2015; McBride, 2005). Beyond 42 the economic impact, this depletion has ecological implications as sea urchins have a 43 key role in the infra-littoral rocky shore areas (Giakoumi et al., 2012; Kitching and 44 Thain, 1983; Privitera et al., 2011; Scheibling, 1986). 45 To deal with overexploitation, echinoculture of several species has been developed 46 worldwide (Andrew et al., 2002): for exemple Paracentrotus lividus in Europe, 47 Loxechinus albus in Chile, Strongylocentrotus spp. in China or S. depressus , S. 48 intermedius and S. nudus in Japan. In Europe, certain constraints remain to be solved for -2- 49 a sustainable and cost-effective industry, notably in the phase of transition between 50 planktonic larvae to a benthic juvenile (Grosjean et al., 1998). Settlement, 51 metamorphosis and post-metamorphosis survival rates are still not high enough to 52 produce juveniles in hatcheries at an industrial-scale (Carbonara et al., 2018; Hannon et 53 al., 2017; Zupo et al., 2018). Researches have been conducted on several sea urchin 54 species to find reliable metamorphosis-inducing-factors for this crucial development 55 stage. Various levels of success have been shown with different species of algae 56 (Carbonara et al., 2018; Castilla-Gavilán et al., 2018b; De la Uz et al., 2013), diatoms 57 and bacterial biofilms (Ab Rahim et al., 2004; Brundu et al., 2016; Dworjanyn and 58 Pirozzi, 2008; Rial et al., 2018; Xing et al., 2007; Zupo et al., 2018), conspecifics 59 (Dworjanyn and Pirozzi, 2008; Gosselin and Jangoux, 1996), and chemical compounds 60 (Carbonara et al., 2018; Pearce and Scheibling, 1990; Swanson et al., 2012). It seems 61 that the most successful metamorphosis-inducing signals are microbial biofilms, 62 whether or not they are linked to the surface of seaweed thalli or inorganic surfaces such 63 as rocks or shells (Hadfield and Paul, 2001). Recent studies have shown the 64 metamorphosis induction effect of benthic diatoms on the culture of P. lividus , 65 obtaining high settlement and survival rates (Rial et al., 2018; Zupo et al., 2018). This 66 zootechnics are also largely used in Japan, China and Chile for sea urchin production in 67 plates covered by natural benthic diatoms biofilms (Ab Rahim et al., 2004; Hagen, 68 1996; McBride, 2005; Rahman et al., 2014; Takahashi et al., 2002; Xing et al., 2007). 69 Moreover, neurotransmitters have been shown to regulate developmental transition in 70 sea urchins, as histamine (Sutherby et al., 2012; Swanson et al., 2012, 2004) and 71 Gamma-Aminobutyric Acid (GABA) (Pearce and Scheibling, 1990; Rahman and 72 Uehara, 2001). -3- 73 The main objective of the present study was to compare the effect of different inducing 74 cues on the metamorphosis of Paracentrotus lividus and to identify those that could be 75 of easy and cheap application. We tested histamine and GABA, two benthic diatoms 76 (Halamphora coffeaeformis and Nitzschia laevis ), a natural benthic biofilm and oyster 77 shells colonized by epiphytic diatoms. 78 Materials and Methods 79 Hatchery of sea urchin larvae 80 Larvae of P. lividus were raised in the Benth’Ostrea Prod aquaculture farm (Bouin, 81 Vendée, France). They were fed on a combined diet consisting of three microalgae 82 species: Isochrysis aff. galbana (clone T-ISO), Rhodomonas sp. and Dunaliella 83 tertiolecta (Castilla-Gavilán et al., 2018a). Larvae were reared in continuous dark at a 84 density of 1 per ml, in 2-m3 conical PVC tanks filled with aerated seawater. A complete 85 water exchange and a thorough cleaning of the tanks were carried out every day. Prior 86 to the experiment, pre-competent larvae were transferred to the laboratory and kept at 87 the same density in an aerated 5 L glass reactor balloon, until competence was achieved. 88 Competence was considered when 75% of larvae had a developed rudiment that was 89 equal or larger than the stomach, as proposed by Kelly et al. (2000). 90 Experimental treatments 91 The chosen treatments for P. lividus competent larvae recruitment assay (see next 92 section) were: 93 - Nitzschia laevis biofilm (NL) 94 - Halamphora coffeaeformis biofilm (HC) 95 - Mix biofilm of both N. laevis and H. coffeaeformis (MIX) -4- 96 - Natural biofilm sampled from refining oyster ponds (NATURAL) 97 - Broken oyster shells 10 g (SHELL) 98 - Histamine 10 -6 M (HIS) 99 - GABA 10 -3 M (GABA) 100 - GABA 10 -3 M + Mix biofilm of both N. laevis and H. coffeaeformis 101 (GABA+MIX) 102 - Control of filtered seawater (FSW) 103 The diatoms N.
Load more

Recommended publications
  • The Gut Microbiome of the Sea Urchin, Lytechinus Variegatus, from Its Natural Habitat Demonstrates Selective Attributes of Micro
    FEMS Microbiology Ecology, 92, 2016, fiw146 doi: 10.1093/femsec/fiw146 Advance Access Publication Date: 1 July 2016 Research Article RESEARCH ARTICLE The gut microbiome of the sea urchin, Lytechinus variegatus, from its natural habitat demonstrates selective attributes of microbial taxa and predictive metabolic profiles Joseph A. Hakim1,†, Hyunmin Koo1,†, Ranjit Kumar2, Elliot J. Lefkowitz2,3, Casey D. Morrow4, Mickie L. Powell1, Stephen A. Watts1,∗ and Asim K. Bej1,∗ 1Department of Biology, University of Alabama at Birmingham, 1300 University Blvd, Birmingham, AL 35294, USA, 2Center for Clinical and Translational Sciences, University of Alabama at Birmingham, Birmingham, AL 35294, USA, 3Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA and 4Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, 1918 University Blvd., Birmingham, AL 35294, USA ∗Corresponding authors: Department of Biology, University of Alabama at Birmingham, 1300 University Blvd, CH464, Birmingham, AL 35294-1170, USA. Tel: +1-(205)-934-8308; Fax: +1-(205)-975-6097; E-mail: [email protected]; [email protected] †These authors contributed equally to this work. One sentence summary: This study describes the distribution of microbiota, and their predicted functional attributes, in the gut ecosystem of sea urchin, Lytechinus variegatus, from its natural habitat of Gulf of Mexico. Editor: Julian Marchesi ABSTRACT In this paper, we describe the microbial composition and their predictive metabolic profile in the sea urchin Lytechinus variegatus gut ecosystem along with samples from its habitat by using NextGen amplicon sequencing and downstream bioinformatics analyses. The microbial communities of the gut tissue revealed a near-exclusive abundance of Campylobacteraceae, whereas the pharynx tissue consisted of Tenericutes, followed by Gamma-, Alpha- and Epsilonproteobacteria at approximately equal capacities.
    [Show full text]
  • (Psammechinus Miliaris) and Their Response to Phenanthrene Exposure
    Marine Environmental Research xxx (2010) 1e9 Contents lists available at ScienceDirect Marine Environmental Research journal homepage: www.elsevier.com/locate/marenvrev Sex-specific biochemical and histological differences in gonads of sea urchins (Psammechinus miliaris) and their response to phenanthrene exposure Sabine Schäfer*,1, Doris Abele, Ellen Weihe, Angela Köhler Alfred Wegener Institute for Polar and Marine Research within the Helmholtz Association, Am Handelshafen 12, 27570 Bremerhaven, Germany article info abstract Article history: Female and male individuals of the same species often differ with respect to their susceptibility to Received 16 August 2010 toxicant stress. In the present study, sea urchins (Psammechinus miliaris) of both sexes were exposed to À À Received in revised form high (150 mgL 1) and environmentally relevant (5 mgL 1) concentrations of phenanthrene over 10 days. 14 October 2010 À While food intake was significantly decreased following exposure to 150 mgL 1 phenanthrene, histo- Accepted 19 October 2010 logical indices (lipofuscin accumulation, fibrosis, oocyte atresia), energetic status (energy charge, sum adenylates, AMP/ATP ratio) as well as ascorbate levels in the gonads showed either little or no effect upon Keywords: phenanthrene exposure. However, most parameters (vitamin C, energy charge, sum adenylates, AMP/ATP Sea urchin fi fi Gonad ratio, ATP and ADP concentrations, lipofuscin content, brosis) signi cantly differed between male and fi Lipofuscin female animals. This study illustrates the dif culties to identify toxic injury in reproductive tissue as it Energy charge may be superimposed by gametogenesis and spawning of gametes. Phenanthrene Ó 2010 Elsevier Ltd. All rights reserved. Ascorbate Sex-specific differences ATP ATP/AMP ratio Gametogenesis 1.
    [Show full text]
  • Marlin Marine Information Network Information on the Species and Habitats Around the Coasts and Sea of the British Isles
    MarLIN Marine Information Network Information on the species and habitats around the coasts and sea of the British Isles Ophiothrix fragilis and/or Ophiocomina nigra brittlestar beds on sublittoral mixed sediment MarLIN – Marine Life Information Network Marine Evidence–based Sensitivity Assessment (MarESA) Review Eliane De-Bastos & Jacqueline Hill 2016-01-28 A report from: The Marine Life Information Network, Marine Biological Association of the United Kingdom. Please note. This MarESA report is a dated version of the online review. Please refer to the website for the most up-to-date version [https://www.marlin.ac.uk/habitats/detail/1068]. All terms and the MarESA methodology are outlined on the website (https://www.marlin.ac.uk) This review can be cited as: De-Bastos, E.S.R. & Hill, J., 2016. [Ophiothrix fragilis] and/or [Ophiocomina nigra] brittlestar beds on sublittoral mixed sediment. In Tyler-Walters H. and Hiscock K. (eds) Marine Life Information Network: Biology and Sensitivity Key Information Reviews, [on-line]. Plymouth: Marine Biological Association of the United Kingdom. DOI https://dx.doi.org/10.17031/marlinhab.1068.1 The information (TEXT ONLY) provided by the Marine Life Information Network (MarLIN) is licensed under a Creative Commons Attribution-Non-Commercial-Share Alike 2.0 UK: England & Wales License. Note that images and other media featured on this page are each governed by their own terms and conditions and they may or may not be available for reuse. Permissions beyond the scope of this license are available here.
    [Show full text]
  • The Marine Fauna of Lundy Ecidnodermata
    Rep. Lundy Fld Soc. 29 (1978) THE MARINE FAUNA OF LUNDY ECIDNODERMATA P. A. TYLER Department of Oceanography, University College, Swansea, S. Wales, U.K. INTRODUCTION The five classes of echinoderms are a conspicuous element of the fauna in truly marine areas. The British echinoderm fauna has been treated in detail by Mortensen (1927). In shelf sea areas they are usually found below LWN tide level with occasional species moving up into the littoral zone. Examples of the dominant extant groups are found in all types of substrates, the ophiuroids and the heart urchins being particularly important in the determination of soft substrate benthic communities (Thorson, 1947). SOURCES OF MATERIAL The collections made by divers during marine surveys of Lundy have pro­ duced a considerable record particularly of the conspicuous epifaunal asteroids, regular echinoids and holothurians. Observations of the less conspicuous in­ faunal ophiuroids and irregular echinoids have been obtained by divers and by benthic surveys using R.V. 'Ocean Crest'. THE LUNDY FAUNA- GENERAL CONSIDERATIONS To date, 24 species of echinoderm have been recorded around Lundy. Of these species only 8 were recorded by Harvey (1950, 1951) at Lundy. The most noteable exceptions to the fauna are Acrocnida brachiata and Spatangus purpureus, both of which have been found further up the Bristol Channel and may be supposed to be found round Lundy where a suitable substrate exists for these infaunal species. A number of species appear to be common all round the island. These include Asterias rubens, Marthasterias glacia/is, Luidia ciliaris, Echinus esculentus and Holothuria forskali. The very rare sea cucumber Lepto­ synapta decaria has been reported as occurring round Lundy (Hoare & Wilson, 1976).
    [Show full text]
  • Sea Urchin Aquaculture
    American Fisheries Society Symposium 46:179–208, 2005 © 2005 by the American Fisheries Society Sea Urchin Aquaculture SUSAN C. MCBRIDE1 University of California Sea Grant Extension Program, 2 Commercial Street, Suite 4, Eureka, California 95501, USA Introduction and History South America. The correct color, texture, size, and taste are factors essential for successful sea The demand for fish and other aquatic prod- urchin aquaculture. There are many reasons to ucts has increased worldwide. In many cases, develop sea urchin aquaculture. Primary natural fisheries are overexploited and unable among these is broadening the base of aquac- to satisfy the expanding market. Considerable ulture, supplying new products to growing efforts to develop marine aquaculture, particu- markets, and providing employment opportu- larly for high value products, are encouraged nities. Development of sea urchin aquaculture and supported by many countries. Sea urchins, has been characterized by enhancement of wild found throughout all oceans and latitudes, are populations followed by research on their such a group. After World War II, the value of growth, nutrition, reproduction, and suitable sea urchin products increased in Japan. When culture systems. Japan’s sea urchin supply did not meet domes- Sea urchin aquaculture first began in Ja- tic needs, fisheries developed in North America, pan in 1968 and continues to be an important where sea urchins had previously been eradi- part of an integrated national program to de- cated to protect large kelp beds and lobster fish- velop food resources from the sea (Mottet 1980; eries (Kato and Schroeter 1985; Hart and Takagi 1986; Saito 1992b). Democratic, institu- Sheibling 1988).
    [Show full text]
  • Annual Macrofauna Production in a Hard-Bottom Reef Community
    J. mar. biol. Ass. U.K. (1985), 65, 713-735 713 Printed in Great Britain ANNUAL MACROFAUNA PRODUCTION IN A HARD-BOTTOM REEF COMMUNITY C. L. GEORGE AND R. M. WARWICK Natural Environment Research Council, Institute for Marine Environment Research, Prospect Place, The Hoe, Plymouth PLi 3DH (Figs. 1-6) Estimates of annual production of the major component species of macrofauna comprising a hard-bottom community at a depth of 41 m in the Bristol Channel have been made. The sea-bed in this region is covered with extensive reefs of the tube-building polychaete Sabellaria spinulosa. Total production is 34-1 g dry wt m~2 y"1, the mean annual biomass 245 g m~2 and the P/B ratio 14. Production is overwhelmingly dominated by the suspension-feeding brittlestar Ophio- thrix fragilis, resulting in a strongly concave species production-rank curve. In addition, there is a diverse fauna of nestling species with slow growth rates which reach maturity at a small body size: production rates for these species are low, but accurate production estimates are sometimes difficult. It is suggested that slow growth rates and small size may result partly from the physical restriction of the reef interstices but principally from the fact that Ophiothrix monopolizes the suspended food resource with an umbrella of feeding arms, and that the infauna of the reef is thus resource-limited. The production ecology at this site is compared with that of other benthic communities in the Bristol Channel. We have limited the detailed presentation of data to a few typical examples; however, data for other species of size frequency, biomass and production are available on request from the authors.
    [Show full text]
  • Phd Lamprianidou Fani 2
    DEVELOPMENT OF A MODEL FOR EVALUATING AND OPTIMIZING THE PERFORMANCE OF INTEGRATED MULTITROPHIC AQUACULTURE (IMTA) SYSTEMS A THESIS SUBMITTED TO THE UNIVERSITY OF STIRLING FOR THE DEGREE OF DOCTOR OF PHILOSOPHY by FANI LAMPRIANIDOU UNIVERSITY OF STIRLING, INSTITUTE OF AQUACULTURE NOVEMBER 2015 In loving memory of Herta 2 ABSTRACT Earth’s population is expected to reach 9 billion by 2050. Ensuring food security for the growing world population is one of today’s society’s major challenges and responsibilities. Aquatic products have the potential to contribute significantly in the growing population’s dietary requirements. Since increasing the pressure on most natural fish stocks is now widely agreed not to be an option, the aquaculture sector needs to grow. The challenge is to increase aquaculture production without depleting natural resources or damaging the environment but also in a financially sustainable way. Integrated Multitrophic Aquaculture (IMTA) is one method of sustainable aquatic production. Integrating bioremediatory organisms that extract particulate organic matter or dissolved inorganic nutrients with monocultures of fed species has the potential of reducing the particulate and soluble waste loads from effluents, whilst producing a low-input protein source that may also increase the farm income. IMTA is a viable solution for mitigating the environmental impact of waste released from fish farms. The fish waste is exploited as a food source for lower trophic, extractive organisms giving an added value to the investment in feed. Studies up to now have shown that under experimental conditions as well as in small-scale commercial studies, various filter-feeding, deposit-feeding and grazing species can ingest fish waste particles.
    [Show full text]
  • Annual Report 2000.Pdf
    REPORT AND ACCOUNTS OF The Scottish Association for Marine Science for the period 1 April 1999 to 31 March 2000 The Dunstaffnage Marine Laboratory, Firth of Lorn, with the Laboratory’s larger research vessel Calanus at the pontoon (right). © John Anderson, Highland Image Contents The cold water coral Lophelia - see page 12 for report Director’s introduction 6 Geochemistry North Sea drill cuttings 8 Baltic Sea System Study 9 Restricted Exchange Environments 10 Deep-Sea Benthic Dynamics Deep-Sea macrofauna 11 Cold water coral Lophelia 12 Continental slope environmental assessment 13 Deep-Water Fish Otolith microchemistry 14 West of Scotland angler fish 15 Deep-water elasmobranchs 16 Invertebrate Biology and Mariculture Sea urchin cultivation 17 Bioaccumulation of chemotherapeutants 18 Microalgal dietary supplements 19 Amnesic shellfish poisoning 19 Marine Biotechnology Natural products research 20 Marine carotenoids 21 Microbial ecotoxicology 21 Microbial Ecology Interannual variability in Antarctic waters 22 Coastal waters of Pakistan 23 Zooplankton Dynamics 24 Clyde time-series 25 Modelling vertical migration 25 Active flux 25 Effects of delousing chemicals 26 Marine Biogenic Trace Gases Light and the dimethylsulphide cycle 27 Sediment trap investigations 28 SAMS/UHIp Marine science degree team 29 Phytoplankton and protozoan dynamics 29 Marine geomorphology of Loch Etive 30 Marine Physics 31 Biogeochemistry 32 Report from the SAMS Honorary Fellows Marine ornithology 33 Geology 34 Data Warehouse progress report 35 4 Annual Report 1999-2000
    [Show full text]
  • The Final Published Version Is Available Direct from the Publisher Website At: 10.1016/J.Lwt.2014.05.016 Link to Author Version on UHI Research Database
    UHI Research Database pdf download summary Extraction and identification of antioxidant polyhydroxynaphthoquinone pigments from the sea urchin, Psammechinus miliaris Powell, C; Hughes, AD; Kelly, MS; Conner, S; McDougall, GJ Published in: LWT-Food Science and Technology Publication date: 2014 The Document Version you have downloaded here is: Early version, also known as pre-print The final published version is available direct from the publisher website at: 10.1016/j.lwt.2014.05.016 Link to author version on UHI Research Database Citation for published version (APA): Powell, C., Hughes, AD., Kelly, MS., Conner, S., & McDougall, GJ. (2014). Extraction and identification of antioxidant polyhydroxynaphthoquinone pigments from the sea urchin, Psammechinus miliaris. LWT-Food Science and Technology, 59(1), 455-460. https://doi.org/10.1016/j.lwt.2014.05.016 General rights Copyright and moral rights for the publications made accessible in the UHI Research Database are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights: 1) Users may download and print one copy of any publication from the UHI Research Database for the purpose of private study or research. 2) You may not further distribute the material or use it for any profit-making activity or commercial gain 3) You may freely distribute the URL identifying the publication in the UHI Research Database Take down policy If you believe that this document breaches copyright please contact us at [email protected] providing details; we will remove access to the work immediately and investigate your claim.
    [Show full text]
  • University of Southampton Research Repository Eprints Soton
    University of Southampton Research Repository ePrints Soton Copyright © and Moral Rights for this thesis are retained by the author and/or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This thesis cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder/s. The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders. When referring to this work, full bibliographic details including the author, title, awarding institution and date of the thesis must be given e.g. AUTHOR (year of submission) "Full thesis title", University of Southampton, name of the University School or Department, PhD Thesis, pagination http://eprints.soton.ac.uk UNIVERSITY OF SOUTHAMPTON FACULTY OF ENGINEERING, SCIENCE AND MATHEMATICS SCHOOL OF OCEAN AND EARTH SCIENCE ACCLIMATION AND PHENOTYPIC PLASTICITY OF ECHINODERM LARVAE IN A CHANGING OCEAN by Nadia Elisa Suárez Bosche Thesis for the degree of Doctor of Philosophy January 2011 A mis padres Mario Cesar Suárez-Arriaga & Elke Amanda Bosche Gracias a Ustedes con su apoyo, ejemplo y cariño este sueño fue posible SCHOOL OF OCEAN AND EARTH SCIENCE NATIONAL OCEANOGRAPHY CENTRE This PhD dissertation by Nadia Elisa Suárez Bosche has been produced under the supervision of the following persons Supervisors: Dr. Debora Iglesias-Rodriguez Prof. Paul Tyler UNIVERSITY OF SOUTHAMPTON ABSTRACT FACULTY OF ENGINEERING, SCIENCE AND MATHEMATICS SCHOOL OF OCEAN AND EARTH SCIENCE Doctor of Philosophy ACCLIMATION AND PHENOTYPIC PLASTICITY OF ECHINODERM LARVAE IN A CHANGING OCEAN Nadia Elisa Suárez Bosche Echinoderms are keystone organisms that have representatives in virtually every marine ecosystem.
    [Show full text]
  • And Psammechinus Miliaris
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by HAL-Université de Bretagne Occidentale The reproductive response of the sea urchins Paracentrotus lividus (G.) and Psammechinus miliaris (L.) to an hyperproteinated macrophytic diet Anne-Gaelle Jacquin, Anne Donval, Jacques Guillou, Sandra Leyzour, Eric Deslandes, Monique Guillou To cite this version: Anne-Gaelle Jacquin, Anne Donval, Jacques Guillou, Sandra Leyzour, Eric Deslandes, et al.. The reproductive response of the sea urchins Paracentrotus lividus (G.) and Psammechinus miliaris (L.) to an hyperproteinated macrophytic diet. Journal of Experimental Marine Biology and Ecology, Elsevier, 2006, 339, pp.43-54. <10.1016/j.jembe.2006.07.005>. <hal-00460554> HAL Id: hal-00460554 http://hal.univ-brest.fr/hal-00460554 Submitted on 1 Mar 2010 HAL is a multi-disciplinary open access L'archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destin´eeau d´ep^otet `ala diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publi´esou non, lished or not. The documents may come from ´emanant des ´etablissements d'enseignement et de teaching and research institutions in France or recherche fran¸caisou ´etrangers,des laboratoires abroad, or from public or private research centers. publics ou priv´es. 1 1 The reproductive response of the sea urchins Paracentrotus 2 lividus (G.) and Psammechinus miliaris (L.) to an 3 hyperproteinated macrophytic diet. 4 JACQUIN Anne-Gaëlle a.b, DONVAL Anne b, GUILLOU Jacques b, 5 LEYZOUR Sandra b, DESLANDES Eric a, GUILLOU Monique b,*. 6 7 a LEBHAM, Université de Bretagne Occidentale, Institut Universitaire 8 Européen de la Mer, Place Nicolas Copernic, 29280 Plouzané, France.
    [Show full text]
  • Maritime Traffic Effects on Biodiversity in the Mediterranean Sea Volume 1 - Review of Impacts, Priority Areas and Mitigation Measures
    Maritime traffic effects on biodiversity in the Mediterranean Sea Volume 1 - Review of impacts, priority areas and mitigation measures Edited by Ameer Abdulla, PhD and Olof Linden, PhD IUCN Centre for Mediterranean Cooperation / IUCN Global Marine Programme cover.indd 2 16/9/08 13:35:23 Maritime traffic effects on biodiversity in the Mediterranean Sea Volume 1 - Review of impacts, priority areas and mitigation measures Edited by Ameer Abdulla, PhD and Olof Linden, PhD portada.indd 1 16/9/08 13:24:04 The designation of geographical entities in this book, and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of the Italian Ministry of Environment, Land and Sea, or IUCN concerning the legal status of any country, territory, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. The views expressed in this publication do not necessarily reflect those of Italian Ministry of Environment, Land and Sea or IUCN. This publication has been made possible by funding from the Italian Ministry of Environment, Land and Sea. This review is a contribution of the Marine Biodiversity and Conservation Science Group of the IUCN Global and Mediterranean Marine Programme. Published by: IUCN, Gland, Switzerland and Malaga, Spain. Copyright: © 2008 International Union for Conservation of Nature and Natural Resources. Reproduction of this publication for educational or other non-commercial purposes is authorized without prior written permission from the copyright holder provided the source is fully acknowledged. Reproduction of this publication for resale or other commercial purposes is prohibited without prior written permission of the copyright holder.
    [Show full text]