Plant Pathol. J. 33(4) : 370-381 (2017) https://doi.org/10.5423/PPJ.OA.01.2017.0017 The Plant Pathology Journal pISSN 1598-2254 eISSN 2093-9280 ©The Korean Society of Plant Pathology Research Article Open Access

Comparative Genome Analysis of Rathayibacter tritici NCPPB 1953 with Rathayibacter toxicus Strains Can Facilitate Studies on Mechanisms of Nematode Association and Host Infection

Jungwook Park1†, Pyeong An Lee2†, Hyun-Hee Lee1, Kihyuck Choi2, Seon-Woo Lee2*, and Young-Su Seo1* 1Department of Microbiology, Pusan National University, Busan 46241, Korea 2Department of Applied Bioscience, Dong-A University, Busan 49315, Korea

(Received on January 25, 2017; Revised on April 12, 2017; Accepted on April 23, 2017)

Rathayibacter tritici, which is a Gram positive, plant Furthermore, many specific genes of R. tritici NCPPB pathogenic, non-motile, and rod-shaped bacterium, 1953 are distributed in substrate-binding proteins causes spike blight in wheat and barley. For suc- for extracellular signals including saccharides, lipids, cessful pathogenesis, R. tritici is associated with An- phosphates, amino acids and metallic cations. These guina tritici, a nematode, which produces seed galls data provides clues on rapid and stable colonization of (ear cockles) in certain plant varieties and facilitates R. tritici for disease mechanism and nematode associa- spread of infection. Despite significant efforts, little tion. research is available on the mechanism of disease or bacteria-nematode association of this bacterium due Keywords : comparative genome, Rathayibacter toxicus, to lack of genomic information. Here, we report the Rathayibacter tritici NCPPB 1953 first complete genome sequence of R. tritici NCPPB 1953 with diverse features of this strain. The whole ge- Handling Associate Editor : Sohn, Kee Hoon nome consists of one circular chromosome of 3,354,681 bp with a GC content of 69.48%. A total of 2,979 genes were predicted, comprising 2,866 protein coding genes As a Gram positive plant pathogen, the genus Rathay- and 49 RNA genes. The comparative genomic analyses ibacter is a member of coryneform bacteria belonging to between R. tritici NCPPB 1953 and R. toxicus strains the family Microbacteriaceae of phylum Actinobacteria, identified 1,052 specific genes in R. tritici NCPPB 1953. previously attributed to genus Clavibacter (Davis et al., Using the BlastKOALA database, we revealed that 1984). Many coryneform phytopathogenic bacteria were the flexible genome of R. tritici NCPPB 1953 is highly initially classified as the genus Corynebacterium (Dow- enriched in ‘Environmental Information Processing’ son, 1942), while many properties of the coryneform system and metabolic processes for diverse substrates. plant pathogenic bacteria were different from those of Corynebacterium sensu stricto (Evtushenko and Doro- †These authors contributed equally to this work as first authors. feeva, 2012). Later, plant pathogenic Corynebacterium *Co-corresponding authors. species were reclassified into the genus Clavibacter due SW Lee to the presence of DAB, which is a particular component Phone) +82-51-200-7551, FAX) +82-51-200-7505 in peptidoglycan cell wall group B (Carlson and Vidaver, E-mail) [email protected] 1982; Davis et al., 1984). In 1993, Zgurskaya and col- YS Seo leagues proposed the genus Rathayibacter such as R. Phone) +82-51-510-2267, FAX) +82-51-514-1778 tritici, R. rathayi, and R. iranicus separating from the ge- E-mail) [email protected] cc This is an Open Access article distributed under the terms of the nus Clavibacter based on DNA-DNA hybridization, che- Creative Commons Attribution Non-Commercial License (http:// motaxonomic studies, and numerical analysis of bacterial creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non- phenotypes (Evtushenko and Dorofeeva, 2012; Zgurskaya commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. et al., 1993). Separation of Rathayibacter species from Clavibacter was further supported by the analyses of 16S Articles can be freely viewed online at www.ppjonline.org. Genomics of Rathayibacter Species 371 rRNA gene sequences (Rainey et al., 1994; Takeuchi and NCPPB 1953 and R. toxicus strains showed a lot of 1,052 Yokota, 1994). The species Rathayibacter toxicus was R. tritici NCPPB 1953-specific genes in flexible genome, also classified in this genus by Sasaki and colleagues in which refers to genes present in two or more organisms or 1998 (Sasaki et al., 1998). Furthermore, these species of specific to a single organism in a pan-genome (Medini et Rathayibacter have been shown to differ absolutely in al., 2005; Sternes and Borneman, 2016). We revealed that many physiological phenotypes, including cell-wall com- these genes are responsible for ‘Environmental Informa- positions, multilocus enzyme profiles, and pigmentations tion Processing’ system and metabolic processes for di- (Davis et al., 1984; De Bruyne et al., 1992; Lee et al., verse substrates, which might explain the rapid and stable 1997; Riley et al., 1988; Zgurskaya et al., 1993). growth of R. tritici rather than R. toxicus. Furthermore, R. tritici has been identified as a causative agent of distinctive genetic features of R. tritici NCPPB 1953 are spike blight, also called yellow ear rot, yellow slime distributed in substrate-binding proteins for extracellular rot, or Tundu disease, in wheat and barley (Paruthi and signals. More research is required to better understand Gupta, 1987). Plant diseases caused by R. tritici result in the pathogenesis of R. tritici and association with the economic losses in many countries worldwide, including nematode, and complete genomic information of R. tritici Australia (Riley and Reardon, 1995), China, Cyprus, Iran NCPPB 1953 will provide a valuable foundation for di- (Bradbury, 1986; Duveiller and Fucikovsky, 1997; Mehta, verse biological experiments. 2014) and Pakistan (Akhtar, 1987). Interestingly, R. tritici shows an association with Anguina tritici, a nematode, Materials and Methods which produces seed galls (ear cockles) in certain plant varieties and facilitates spread of R. tritici infection (Pa- Growth conditions and genomic DNA preparation. ruthi and Bhatti, 1985). Intact seed galls produced by Pure culture of R. tritici NCPPB 1953 was grown on nutri- nematodes display the greatest grain loss due to develop- ent broth yeast extract (NBY) media (8 g nutrient broth, 2 ment of R. tritici (Fattah, 1988). Similarly, a bacterium, R. g yeast extract, 2 g K2HPO4, 0.5 g KH2PO4, 2.5 g glucose toxicus, causing a gumming disease and ryegrass toxicity per liter, followed by autoclaving and supplementation is associated with a nematode vector (Riley and Ophel, with 1 ml of 1 M MgSO4·7H2O) media for 96 h at 25°C 1992). Compared to R. tritici, R. toxicus is commonly (Zgurskaya et al., 1993). For extraction of genomic DNA, found in annual ryegrass with A. funesta as a nematode the pellet of bacterial cells grown on the agar plate was vector, rabbit-foot grass and annual browngrass with un- harvested with sterilized water. DNA was subsequently described Anguina species. While host plants for R. toxi- isolated using the Promega Wizard Genomic DNA Puri- cus are limited to ryegrasses in nature, it is likely that R. fication Kit (Promega, Madison, WI, USA) following the toxicus is not host specific experimentally (Agarkova et standard protocol provided by the manufacturer. DNA al., 2006). In addition to unique host plants for R. toxicus, was visualized in ethidium bromide-stained 0.7% agarose R. toxicus is different from R. tritici for the production of gel, and the concentration and purity of DNA were deter- glycolipid toxins, known as corynetoxins, in the infected mined by a NanoDropTM spectrophotometer. ryegrass, and thus causes a lethal toxicosis in the animal that consumed the infected plants (Agarkova et al., 2006; Genome sequencing of R. tritici NCPPB 1953. Third Jago and Culvenor, 1987). Both in R. tritici and R. toxi- generation DNA sequencing of the SMRT technology cus, bacterial association with a nematode vector could be (Pacific Biosciences, Menlo Park, CA, USA) was used used as a good model of interaction between plant patho- to establish the complete genome sequence of R. tritici gens and nematodes for successful pathogenesis. Despite NCPPB 1953 (Chin et al., 2013). A total of 150,292 raw significant efforts and interesting association between reads containing an average of 7,236 bp were generated bacterial pathogen and nematodes, little research is avail- by a 20-kb insert SMRTbell standard library. To correct able on the mechanism of disease or bacteria-nematode errors, long reads were selected as seeds, and other short association of this bacterium due to lack of genomic in- reads were aligned into seeds by the basic local align- formation. ment with successive refinement step (Chaisson and Characterising the complete genome of R. tritici could Tesler, 2012). After filtration, post-filtered reads included help to resolve these gaps in our understanding. Here, we 86,775 reads and represented an average of 11,144 bp report the first whole genome sequence of R. tritici NCP- with a quality of 0.852. The de novo assembly of post- PB 1953 isolated from wheat seeds. The whole genome filtered reads was conducted using the HGAP pipeline of R. tritici NCPPB 1953 harbours one circular chromo- from SMRT-Analysis with default parameters (Chin et some of 3,354,681 bp with 2,866 protein coding genes. al., 2013). Circularization was verified, and overlapping The comparative genomic analysis between R. tritici ends were trimmed (Kopf et al., 2014). Assembly resulted 372 Park et al. in a single contig with a circular form of 3,354,681 bp. Comparative genome analysis. For this analyses, three Subsequently, to evaluate assembly quality, all reads were complete genome of R. tritici NCPPB 1953 (acces- mapped back to the chromosomal sequence according sion no. CP015515), R. toxicus WAC3373 (accession to the RS_Resequencing protocol. Final results indicate no. CP013292), and R. toxicus 70137 (accession no. bases called value of 99.88%, consensus concordance of CP010848) were obtained from GenBank (Benson et al., 100%, and 187.88-fold coverage. 2000). We employed the Mauve tool to compare whole genome sequences (Darling et al., 2004). The progressive Gene annotation of R. tritici NCPPB 1953. Genomic Mauve algorithm was used to identify genomic rearrange- rRNAs and tRNAs were predicted using RNAmmer (Lag- ments among whole genome sequences. The alignment esen et al., 2007) and tRNAscan-SE (Lowe and Eddy, was performed using default parameters. 1997), respectively. All open reading frames (ORFs) and pseudogenes were predicted using Glimmer (Delcher et Pan-genome analysis. Genomic information of R. al., 1999), GeneMarkHMM (Lukashin and Borodovsky, tritici NCPPB 1953 was formatted for a reference data- 1998) and Prodigal (Hyatt et al., 2010). Predicted ORFs base using the formatdb tool of BLAST (Altschul et al., with nucleotides were translated into amino acid sequenc- 1990). The local BLASTp compared total protein genes es and then searched using the BLAST algorithm (Altschul of R. toxicus WAC3373 and R. toxicus 70137 to refer- et al., 1990) against the NCBI-NR (Benson et al., 2000), ence database of R. tritici NCPPB 1953 through amino Pfam (Finn et al., 2016) and UniProt (UniProt Consor- acid sequences. The BLAST outputs were filtered with tium, 2013) databases for a description of each protein. significant criteria of 50% coverage, 50% identity, and In addition, the KEGG (Kanehisa and Goto, 2000) and an e-value cutoff of 1.0 × 10–5 to exclude random hits COG (Galperin et al., 2015) databases were used to con- (Anderson and Brass, 1998). By sorting e-value, the best struct functional categories in accordance with biological matched subject was selected from total results per each systems. Genes in internal clusters were detected using query. Subsequently, to investigate biological meaning BLASTclust (Alva et al., 2016) with significant cutoffs of R. tritici NCPPB 1953-specific genes, we used the of 70% coverage and 30% identity. Signal peptides and BlastKOALA tool as an automatic annotation server for transmembrane helices were based on SignalP (Petersen genome and metagenome sequences (Kanehisa et al., et al., 2011) and TMHMM (Krogh et al., 2001), respec- 2016). The BlastKOALA performed KEGG orthology tively. CRISPRFinder was used to identify the CRISPR assignments to characterize individual gene functions and (Grissa et al., 2007). Predicted genes were compared with reconstruction of KEGG pathways and modules in R. trit- the Pathogen-Host Interaction (PHI) database (Urban ici NCPPB 1953. Also, we constructed networks of some et al., 2015) using the BLASTp method and an e-value genes related to transport system using the Cytoscape tool cutoff of 1.0 × 10–5 (Buiate et al., 2017). The annotation (http://www.cytoscape.org/). results were verified using the Artemis (Rutherford et al., 2000). Results and Discussion

Nucleotide sequence accession number of R. tritici General features of R. tritici NCPPB 1953. R. tritici NCPPB 1953. The complete genome of R. tritici NCPPB NCPPB 1953 is a Gram positive, non-motile, and rod- 1953 has been deposited in GenBank (Benson et al., shaped bacterium. Its size is 1.1–1.6 μm long and 0.5–0.6 2000) under accession number CP015515. μm wide (Fig. 1). On an aerobic NBY agar plate, R. tritici NCPPB 1953 formed round colonies and produced yel- Phylogenetic analysis. To evaluate evolutionary dis- low pigment within 96 h at 25°C (Fig. 1D) and could tances among R. tritici NCPPB 1953 and closely related grow in a pH range of 6–8. This strain was a non-motile bacteria in the genus Rathayibacter, we collected all 16S bacterium and cells of R. tritici NCPPB 1953 were short, rRNA sequences of 26 bacteria from the SILVA database. straight or slightly curved rods with blunt ends. Cells oc- The ClustalW tool was used to align all sequences with curred singly, in pairs or sometimes in aggregates (Fig. default parameters (Larkin et al., 2007). Phylogenetic 1A, B). This is likely because divided cells by binary fis- tree was conducted by the Maximum Likelihood algo- sion failed to separate after septum formation. Cells were rithm based on the Tamura-Nei model as implemented in surrounded by a capsule and the capsular material some- MEGA 7.0 tool (Kumar et al., 2016). The corresponding times exhibited distinct extracellular outer layers (Fig. parameter of the Maximum Likelihood algorithm was 1C; black arrows). Bacterial colonies were often variable set as ‘complete deletion’. The evolutionary test was per- in size, probably because of capsules and bacterial cell formed by bootstrap analysis with 1,000 replications. aggregates. Genomics of Rathayibacter Species 373

A B

500 nm 500 nm

C D

1mm 1cm

Fig. 1. Image of transmission electron microscope (TEM) photomicrograph (A–C) and the appearance of colony morphology on solid media (D) of Rathayibacter tritici NCPPB 1953. Cells were grown in NBY agar plate for 96 h. The magnification rates of TEM for A–C were 15,000×, 25,000×, and 10,000×, respectively. Bacterial capsules with extracellular outer layer were observed (black ar- rows).

Phylogenetic analysis of R. tritici NCPPB 1953 with Table 1). closely-related bacteria species. Phylogenetic analysis was performed using the 16S rRNA genes of 26 different General genomic features of R. tritici NCPPB 1953. bacteria in the genus Rathayibacter: 3 strains, R. caricis; The complete genome of R. tritici NCPPB 1953 consists 3 strains, R. festucae; 3 strains, R. iranicus; 3 strains, R. of a single circular form of 3,354,681 bp with 69.48% GC rathayi; 5 strains, R. toxicus; 9 strains, R. tritici (Fig. 2). content. There are 2 rRNA operons, 43 tRNAs, and 64 All 16S rRNA sequences were downloaded from the pseudogenes (Table 1). The chromosome contains 2,866 SILVA (Quast et al., 2013), providing information on protein-coding genes. Of these, 1,712 proteins are as- small (16S/18S, SSU) and large (23S/28S, LSU) subunit signed to functional annotations, whereas 1,154 proteins ribosomal RNA databases. Pairwise distances were es- are considered as hypothetical proteins. Table 2 shows the timated using the Maximum Composite Likelihood ap- number of genes associated with general COG functional proach (Supplementary Table 1). All positions containing categories. The ‘General function prediction only’ (9.70%) gaps and missing data were eliminated. There were a total represents the largest category, followed by ‘Amino acid of 994 positions in the final dataset. As a result, R. tritici transport and metabolism’ (8.27%), ‘Carbohydrate trans- NCPPB 1953 was grouped with most of R. tritici strains. port and metabolism’ (8.20%), ‘Transcription’ (6.84%), R. tritici ATCC 11402 and R. tritici DSM 7486T repre- and ‘Translation, ribosomal structure, and biogenesis’ sented the closest bacteria as both distance value 0.000, (6.42%). A complete genomic map of R. tritici NCPPB followed by R. rathayi DSM 7485T (distance value 0.001), 1953 was constructed using the CGView tool in Fig. 3. R. tritici ICPB70004 (distance value 0.001), and R. irani- So far, it is hard to find virulence factors of R. tritici cus DSM 7484T (distance value 0.003) (Supplementary NCPPB 1953, since there is a lack of research on the 374 Park et al.

Fig. 2. 16S rRNA phylogenetic tree showing the relationships of Rathayibacter tritici NCPPB 1953 (shown in bold print) with other members in the genus Rathayibacter. Sequences were aligned using ClustalW and the phylogeny was inferred from the alignment of 1,267 bp of 16S rRNA using the Maximum Likelihood method based on the Tamura-Nei model with MEGA 7.0 software. The boot- strap consensus tree from 1,000 replicates was performed to assess the support of the clusters. The scale bar indicates 0.01 nucleotide change per nucleotide position. The GenBank accession numbers are shown in parentheses. pathogenesis and virulence mechanisms of R. tritici. tary Table 2. Most of putative virulence genes in R. tritici We therefore performed detection of putative virulence NCPPB 1953 were distributed in many plant pathogenic genes in the R. tritici NCPPB 1953 genome using the bacteria and fungi. Magnaporthe oryzae, which causes a PHI database that archives the list of experimentally veri- serious disease affecting rice (Wilson and Talbot, 2009), fied pathogenicity, virulence, and effectors from diverse represented the largest category matched with 58 genes microbes (Urban et al., 2015). A total of 676 putative of R. tritici NCPPB 1953, followed by Xanthomonas virulence genes were identified by the significant cutoff citri (matched with 36 genes), Fusarium graminearum of 1.0 × 10–5 e-value (Table 1). R. tritici NCPPB 1953 had (matched with 32 genes), and Pseudomonas cichorii about 200 more virulence genes than R. toxicus strains (447 (matched with 21 genes). In particular, the X. citri mutant virulence genes in WAC3373 and 445 virulence genes of the gene pstB (encoding the ABC phosphate transport- in 70137). Detailed information with putative virulence er subunit), which are matched with 30 genes of R. tritici genes of R. tritici NCPPB 1953 was listed in Supplemen- NCPPB 1953, revealed a complete absence of symptoms Genomics of Rathayibacter Species 375

Table 1. Genome statistics in Rathayibacter tritici NCPPB 1953 and Rathayibacter toxicus strains Attribute R. tritici NCPPB 1953 R. toxicus WAC3373 R. toxicus 70137 Genome size (bp) 3,354,681 2,346,032 2,328,288 DNA coding (bp) 2,830,330 1,970,833 1,957,820 DNA G + C (bp) 2,330,883 1,442,241 1,431,482 DNA scaffolds 1 1 1 Total genes 2,979 2,137 2,120 Protein coding genes 2,866 1,999 1,993 RNA genes 49 51 51 Pseudo genes 64 75 70 Genes in internal clusters 780 309 303 Genes with functional prediction 1,712 1,174 1,158 Genes assigned to COGs 2,238 1,497 1,499 Genes with Pfam domains 2,159 1,575 1,572 Genes with signal peptides 218 117 117 Genes with transmembrane helices 694 497 495 CRISPR repeats 0 2 2 Putative virulence genes 676 447 445

Table 2. A distribution of genes associated with COG categories in Rathayibacter tritici NCPPB 1953 Code Value Percentage Description J 184 6.42 Translation, ribosomal structure and biogenesis A 1 0.03 RNA processing and modification K 196 6.84 Transcription L 126 4.40 Replication, recombination, and repair B 0 0.00 Chromatin structure and dynamics D 35 1.22 Cell cycle control, cell division, chromosome partitioning V 68 2.37 Defense mechanisms T 113 3.94 Signal transduction mechanisms M 149 5.20 Cell wall/membrane biogenesis N 16 0.56 Cell motility U 20 0.70 Intracellular trafficking and secretion O 109 3.80 Posttranslational modification, protein turnover, chaperones C 110 3.84 Energy production and conversion G 235 8.20 Carbohydrate transport and metabolism E 237 8.27 Amino acid transport and metabolism F 91 3.18 Nucleotide transport and metabolism H 147 5.13 Coenzyme transport and metabolism I 116 4.05 Lipid transport and metabolism P 130 4.54 Inorganic ion transport and metabolism Q 76 2.65 Secondary metabolites biosynthesis, transport, and catabolism R 278 9.70 General function prediction only S 130 4.54 Function unknown - 628 21.91 Not in COGs

(Moreira et al., 2015). It was also reported that the gene cope with the cytotoxic environment within host (Gupta ABC4 (matched with 16 genes of R. tritici NCPPB 1953) and Chattoo, 2008). This data suggests that many viru- of ABC superfamily of membrane transporters is required lence genes in R. tritici NCPPB 1953 are implicated to for the pathogenicity of M. oryzae, helping the fungus to play a role in the membrane transporters to control efflux 376 Park et al. and influx of host metabolites for their pathogenicity. major biological properties compared to R. tritici. For examples, a gumming disease was reported as a com- The comparative genomic analysis between R. tritici mon plant disease caused by both R. tritici and R. toxicus NCPPB 1953 and R. toxicus strains. R. toxicus shares (Agarkova et al., 2006; Arif et al., 2016). Also, R. toxicus uses several species of Anguina as the vector system to carry themselves into the plant host like R. tritici (Price et al., 1979; Riley, 1992). Riley and Reardon (1995) dis- cussed the potential of R. tritici as a biological control for R. toxicus because of co-occurrence of two bacteria. Thus, R. toxicus is the important source for comparative analysis with R. tritici and we intended to find distinc- tive genetic features of R. tritici NCPPB 1953 through

3,000 kbp comparative genomic analysis between R. tritici and R. 500 kbp toxicus. Among R. toxicus strains, there are two complete

2,500 kbp genomes in the GenBank (Benson et al., 2000) including 1,000 kbp R. toxicus WAC3373 and R. toxicus 70137 under acces-

2,000 kbp 1,500 kbp sion number CP013292 and CP010848, respectively. Based on the pairwise BLAST comparison of the 16S rRNA gene sequences, R. tritici NCPPB 1953 was closely related to R. toxicus, resulting in 100% coverage and 98% identity in both comparisons with R. toxicus WAC3373 and R. toxicus 70137. A similar distribution of rRNA and tRNA genes was maintained as follows: 6 rRNAs and 43 tRNAs, R. tritici NCPPB 1953; 6 rRNAs and 45 tRNAs, R. toxicus WAC3373; 6 rRNAs and 45 tRNAs, R. toxicus Fig. 3. Graphical circular map of Rathayibacter tritici NCPPB 70137. 1953 chromosome displaying relevant genetic features. From centre to outside: 1, positive (green) and negative (yellow) of However, there are different features between R. tritici GC skew; 2, GC content (black); 3, CDSs on reverse strand and R. toxicus in the genome-wide level. GC contents (blue); 4, CDSs on forward strand (red); 5, pseudogenes (grey); of whole genome sequence showed a large discrepancy 6, tRNAs (brown); 7, rRNAs (pink). This figure was built using between R. tritici (GC content 69.5%) and R. toxicus (GC the CGView tool. contents 61.5% in WAC3373 and 61.5% in 70137). Above

Fig. 4. Whole genome alignment of Rathayibacter tritici NCPPB 1953, Rathayibacter toxicus WAC3373, and Rathayibacter toxicus 70137. The top, middle, and bottom sequences represent each genomic sequence of R. tritici NCPPB 1953, R. toxicus WAC3373, and R. toxicus 70137, respectively. Identically coloured boxes by same lines are locally collinear blocks, indicating homologous and conservative regions in both genomes. Coloured bars inside boxes are related to the level of sequence similarities. Numbers above the map show nucleotide positions. Genomics of Rathayibacter Species 377 all, R. tritici NCPPB 1953 harbours a larger genomic size were identified through the BlastKOALA algorithm to in- (about 3.35 Mbp) and more genes (2,979 genes), when fer high-level functions of gene clusters (Kanehisa et al., compared to R. toxicus WAC3373 (about 2.35 Mbp and 2016). As a result, the highest enriched system was ‘En- 2,137 genes) and R. toxicus 70137 (about 2.33 Mbp and vironmental Information Processing’ (72 genes) related to 2,120 genes). Indeed, we revealed the dynamic genomic sensing signals and responses to diverse stimuli (Fig. 5). rearrangement of whole genome sequence between R. Also, many metabolic processes for substrates were ob- tritici and R. toxicus using the Mauve aligner (Darling et served in enriched systems as follows: ‘Carbohydrate me- al., 2004). In Fig. 4, R. tritici NCPPB 1953 shared a total tabolism’, 28 genes; ‘Amino acid metabolism’, 21 genes; of 58 locally collinear blocks (LCBs), which are highly homologous regions among genome sequences, in com- parison to R. toxicus. Although these LCBs covered most Table 3. Modules related to ‘Environmental Information Pro- parts of the whole genome in R. tritici NCPPB 1953, cessing’ in Rathayibacter tritici NCPPB 1953-specific genes most of LCBs showed sporadic inversion and irregular Gene genomic rearrangement caused by local and/or large-scale KEGG ID Module counts changes in genetic loci. In addition to the variable rear- rangement, we confirmed additional genetic differences rtn_M00196 Multiple sugar transport system 8 in R. tritici NCPPB 1953 such as non-matched regions rtn_M00206 Cellobiose transport system 2 and some LCBs of low similarities. Medini et al. (2005) rtn_M00207 Putative multiple sugar transport 1 reported that conserved core regions of microbial genome system are responsible for basic aspects of the biology related to rtn_M00209 Osmoprotectant transport system 1 essential phenotypes and growth, whereas each flexible rtn_M00212 Ribose transport system 2 genome allows bacteria to obtain supplementary bio- rtn_M00216 Multiple sugar transport system 4 rtn_M00218 Fructose transport system 2 chemical functions as selective advantages. Consequently, rtn_M00221 Putative simple sugar transport 3 this data suggests that R. tritici NCPPB 1953 have needed system additional genetic elements and functions, which could rtn_M00233 Glutamate transport system 1 provide effective physiological activity in accordance rtn_M00236 Putative polar amino acid transport 1 with specific environment and conditions. system rtn_M00238 D-Methionine transport system 1 Functional analyses of flexible genome in R. tritici rtn_M00240 Iron complex transport system 1 NCPPB 1953 compared to R. toxicus strains. To inves- rtn_M00243 Manganese/iron transport system 1 tigate the biological meaning of distinctive genetic ele- rtn_M00252 Lipooligosaccharide transport system 2 ments in R. tritici NCPPB 1953, we performed the pan- rtn_M00254 ABC-2 type transport system 6 genome analysis of R. tritici NCPPB 1953 compared to rtn_M00299 Spermidine/putrescine transport 2 R. toxicus WAC3373 and R. toxicus 70137, leading to system identification of 1,052 R. tritici NCPPB 1953-specific rtn_M00436 Sulfonate transport system 2 genes (Supplementary Fig. 1). Furthermore, 1,052 genes rtn_M00813 Lantibiotic transport system 2

10 10 12 Environmental Information Processing Unclassified 14 72 Genetic information processing Carbohydrate metabolism 14 Amino acid metabolism Nucleotide metabolism Enzyme families 15 Metabolism of cofactors and vitamins Energy metabolism Fig. 5. Summary of biological sys- Cellular processes tems in Rathayibacter tritici NCPPB 21 Human diseases 1953-specific genes. A distribution of the KEGG systems is displayed 67 as a pie-chart. The number of gene 28 counts is shown inside each pie of 32 categories. Total 11 KEGG terms are displayed. 378 Park et al.

Substrate ATP binding Permease binding protein protein

Alkanesulfonate SsuA SsuC SsuB

Spermidine/Putrescine PotD PotB PotA

MsmF Raffinose/Stachyose/Melibiose MsmE MsmK MsmG

CebF Cellobiose CebE MsiK CebG

Multiple sugar ChvE GguB GguA Fig. 6. The network of Rathayibacter tritici NCPPB 1953 in KEGG trans- Ribose/Autoinducer2/D-Xylose RbsB RbsC RbsA porter. Pathway maps of KEGG transporter system were constructed Fructose FrcB FrcC by the Cytoscape tool. The KEGG database was used to download the GluC Glutamate GluB GluA data source. There are two color types GluD in the rectangle node: red, genes belonging to flexible genome of R. D-Methionine MetQ MetN tritici NCPPB 1953; green, genes Flexible genome Core genome belonging to the core genome among Iron complex FhuD FhuB FhuC Substrate R. tritici NCPPB 1953, R. toxicus WAC3373, and R. toxicus 70137.

‘Nucleotide metabolism’, 15 genes; ‘Metabolism of co- Flexible genome of R. tritici NCPPB 1953 was particu- factors and vitamins’, 14 genes; ‘Energy metabolism’, 12 larly distributed in substrate-binding proteins of transport genes (Fig. 5). These results indicate that flexible genome system (Fig. 6), which is a key determinant of the extra- in R. tritici NCPPB 1953 have been mainly developed to cellular signals and high affinity of uptake systems (Maq- sense and process diverse substrates from the environ- bool et al., 2015). In this regard, there are interesting re- ment. ports on the flexible growth and colonization of R. tritici In general, the environment with host condition is far than R. toxicus (Bradbury, 1973; Riley and Ophel, 1992). away from the optimal growth conditions for bacteria In 1992, Riley and Ophel reported that R. tritici grows than experimental conditions with rich media due to more rapidly than R. toxicus in culture (Riley and Ophel, multiple stresses (Shimizu, 2013; Yadeta and Thomma, 1992). It was reported that R. tritici successfully colonizes 2013). The acquisition of nutrients of plant pathogens in in plant hosts such as the gummosis of seed heads rather hostile environment is a fundamental challenge against an than R. toxicus (Bradbury, 1973). Therefore, we suggests extremely low level of organic and inorganic compounds that distinctive genetic features in flexible genome help (Yadeta and Thomma, 2013). Pathogens have to need R. tritici NCPPB 1953 to establish the stable growth and the ability to sense, process, and respond to a variety of colonization in diverse environments through effective substrates and stresses for survival (Shimizu, 2013). Un- recognition of substrates. Further studies could lead to a til now, diverse mechanisms for sensing and processing better understanding of interactions between R. tritici and exogenous metabolites within host have been intensively nematode as well as R. tritici and plant hosts. studied in many pathogens (Divon et al., 2006; Jones and Wildermuth, 2011; Wooldridge and Williams, 1993). Our Acknowledgments module enrichments showed that most genes in the ‘Envi- ronmental Information Processing’ system are associated This research was supported by the Research of Animal with recognition and transport of many substrates, includ- and Plant Quarantine Agency, South Korea and with the ing saccharides, lipids, phosphates, amino acids, metallic support of “Cooperative Research Program for Agricul- cations, minerals, organic ions, and vitamins (Table 3). ture Science & Technology Development (PJ01110502)”, Genomics of Rathayibacter Species 379

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Pairwise distance matrix derived from 26 different bacteria in the genus Rathayibacter 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 1 R. tritici NCPPB 1953 [CP015515] 2 R. caricis 0312MAR25Y2 [LN774722] 0.004034 3 R. caricis 1RN-3C1 [EU379250] 0.015280 0.013234 4 R. caricis VKM Ac-1799T [AF159364] 0.006062 0.004039 0.015297 5 R. festucae 3A6-2009 [KC469952] 0.004040 0.006071 0.017374 0.008109 6 R. festucae DSM 15932T [AM410683] 0.005056 0.007092 0.018419 0.009134 0.001007 7 R. festucae VKM Ac-1390 [AF159365] 0.005056 0.007092 0.018419 0.009134 0.001007 0.002017 8 R. iranicus DSM 7484T [AM410684] 0.003026 0.006065 0.017354 0.008100 0.006073 0.007094 0.007094 9 R. iranicus ICPB70005 [FJ595101] 0.003026 0.006065 0.017354 0.008100 0.006073 0.007094 0.007094 0.000000 10 R. iranicus VKM Ac-1602 [KC188341] 0.003026 0.006065 0.017354 0.008100 0.006073 0.007094 0.007094 0.000000 0.000000 11 R. rathayi ATCC 13659 [U96186] 0.076029 0.079426 0.091022 0.081724 0.080645 0.081825 0.081825 0.077144 0.077144 0.077144 12 R. rathayi DSM 7485T [X77439] 0.001006 0.005045 0.016303 0.007075 0.005052 0.006069 0.006069 0.004035 0.004035 0.004035 0.077124 13 R. rathayi JCM9307 [D45062] 0.008117 0.012206 0.023629 0.014265 0.012223 0.013257 0.013257 0.011188 0.011188 0.011188 0.082985 0.007100 14 R. toxicus 70137 [CP010848] 0.025615 0.028753 0.038305 0.030861 0.028794 0.029858 0.029858 0.025625 0.025625 0.025625 0.086048 0.024583 0.030918 15 R. toxicus DSM 7488T [AUDF01000011] 0.025615 0.028753 0.038305 0.030861 0.028794 0.029858 0.029858 0.025625 0.025625 0.025625 0.086048 0.024583 0.030918 0.000000 16 R. toxicus FH142 [LBFI01000038] 0.025615 0.028753 0.038305 0.030861 0.028794 0.029858 0.029858 0.025625 0.025625 0.025625 0.086048 0.024583 0.030918 0.000000 0.000000 17 R. toxicus JCM 9669 [LC066140] 0.025615 0.028753 0.038305 0.030861 0.028794 0.029858 0.029858 0.025625 0.025625 0.025625 0.086048 0.024583 0.030918 0.000000 0.000000 0.000000 18 R. toxicus WAC3373 [CP013292] 0.025615 0.028753 0.038305 0.030861 0.028794 0.029858 0.029858 0.025625 0.025625 0.025625 0.086048 0.024583 0.030918 0.000000 0.000000 0.000000 0.000000 19 R. tritici OS-16.a [AM237343] 0.004040 0.006071 0.017374 0.008109 0.000000 0.001007 0.001007 0.006073 0.006073 0.006073 0.080645 0.005052 0.012223 0.028794 0.028794 0.028794 0.028794 0.028794 20 R. tritici 6J-5a [EU379299] 0.010140 0.008107 0.019448 0.010152 0.012209 0.013242 0.013242 0.012195 0.012195 0.012195 0.077082 0.011158 0.018408 0.020387 0.020387 0.020387 0.020387 0.020387 0.012209 21 R. tritici ATCC 11402 [U96185] 0.000000 0.004034 0.015280 0.006062 0.004040 0.005056 0.005056 0.003026 0.003026 0.003026 0.076029 0.001006 0.008117 0.025615 0.025615 0.025615 0.025615 0.025615 0.004040 0.010140 22 R. tritici DSM 7486T [AM410685] 0.000000 0.004034 0.015280 0.006062 0.004040 0.005056 0.005056 0.003026 0.003026 0.003026 0.076029 0.001006 0.008117 0.025615 0.025615 0.025615 0.025615 0.025615 0.004040 0.010140 0.000000 23 R. tritici ICPB70004 [FJ595102] 0.001007 0.005049 0.016318 0.007081 0.005056 0.006074 0.006074 0.004039 0.004039 0.004039 0.077198 0.002015 0.009142 0.026672 0.026672 0.026672 0.026672 0.026672 0.005056 0.011168 0.001007 0.001007 24 R. tritici IHBB 9484 [KR085826] 0.005045 0.003025 0.014257 0.005050 0.007086 0.008107 0.008107 0.007077 0.007077 0.007077 0.076029 0.006055 0.013227 0.029789 0.029789 0.029789 0.029789 0.029789 0.007086 0.009124 0.005045 0.005045 0.006061 25 R. tritici SAFR-009 [AY167853] 0.004040 0.006071 0.017374 0.008109 0.000000 0.001007 0.001007 0.006073 0.006073 0.006073 0.080645 0.005052 0.012223 0.028794 0.028794 0.028794 0.028794 0.028794 0.000000 0.012209 0.004040 0.004040 0.005056 0.007086 26 R. tritici Tibetlhz-21 [JX827198] 0.004035 0.006065 0.017354 0.008100 0.006073 0.007094 0.007094 0.006066 0.006066 0.006066 0.078240 0.005046 0.012209 0.026641 0.026641 0.026641 0.026641 0.026641 0.006073 0.010138 0.004035 0.004035 0.005050 0.007077 0.006073 Supplementary Table 2. Gene list of putative virulence in R. tritici NCPPB 1953 R. tritici NCPPB 1953 Hits on the PHI-base database E -valueb Locus ID Product Gene Pathogen Mutant phenotype Accession IDa A6122_0007 DNA gyrase subunit A GyrA Burkholderia glumae chemistry target: sensitivity to chemical PHI:824 0 A6122_0011 DNA mismatch repair protein MutT PSPTO_2696 Pseudomonas syringae unaffected pathogenicity PHI:3119 0.00000007 A6122_0013 peptidylprolyl isomerase BCP1 Botrytis cinerea reduced virulence PHI:277 8.00E-32 A6122_0019 hypothetical protein pabaA Aspergillus fumigatus reduced virulence PHI:178 6.00E-14 A6122_0021 serine/threonine protein kinase stk Streptococcus suis reduced virulence PHI:4155 1.00E-74 A6122_0022 serine/threonine protein kinase stk Streptococcus suis reduced virulence PHI:4155 2.00E-58 A6122_0033 ABC transporter ATP-binding protein ABC3 Magnaporthe oryzae loss of pathogenicity PHI:2042 2.00E-11 A6122_0036 potassium transporter KtrB kdpB Pseudomonas aeruginosa reduced virulence PHI:4712 0 A6122_0038 histidine kinase rcsC11 Salmonella enterica mixed PHI:3011 5.00E-19 A6122_0039 two-component system response regulator PhoP Xanthomonas oryzae reduced virulence PHI:1144 5.00E-41 A6122_0040 S-ribosylhomocysteine lyase AI-2 Enterococcus faecalis increased virulence (hypervirulence) PHI:4577 2.00E-32 A6122_0043 heme ABC transporter ATP-binding protein ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 0.0000006 A6122_0049 hypothetical protein Rv3597c Mycobacterium tuberculosis unaffected pathogenicity PHI:3632 2.00E-18 A6122_0053 transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 4.00E-10 A6122_0060 quinone oxidoreductase PKS1 Cochliobolus heterostrophus reduced virulence PHI:55 5.00E-21 A6122_0063 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 1.00E-33 A6122_0066 transporter, major facilitator family protein MgMfs1 Zymoseptoria tritici chemistry target: sensitivity to chemical PHI:1161 4.00E-20 A6122_0067 hypothetical protein pobA Xanthomonas campestris reduced virulence PHI:5404 0.00000009 A6122_0070 aldo/keto reductase CSH1 Candida albicans reduced virulence PHI:419 2.00E-23 A6122_0085 3-oxoacyl-ACP reductase fabG1 Ralstonia solanacearum lethal PHI:5271 1.00E-28 A6122_0096 non-ribosomal peptide synthetase Cif_B Pseudomonas cichorii reduced virulence PHI:4836 2.00E-70 A6122_0099 hypothetical protein SidI Aspergillus fumigatus reduced virulence PHI:2321 2.00E-17 A6122_0106 Tyrocidine synthase 3 Cif_B Pseudomonas cichorii reduced virulence PHI:4836 5.00E-50 A6122_0110 oxidoreductase entA Bacillus cereus reduced virulence PHI:4077 5.00E-32 A6122_0111 hypothetical protein dhbF Paenibacillus larvae unaffected pathogenicity PHI:3274 2.00E-123 A6122_0112 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 5.00E-41 A6122_0117 LacI family transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 2.00E-23 A6122_0119 hypothetical protein rfbC Xanthomonas citri reduced virulence PHI:2481 1.00E-11 A6122_0130 LuxR family transcriptional regulator degU Listeria monocytogenes reduced virulence PHI:4690 3.00E-31 A6122_0144 peptidase M13 Zmp1 Mycobacterium tuberculosis increased virulence (hypervirulence) PHI:4957 0 A6122_0155 hypothetical protein radC Streptococcus pneumoniae reduced virulence PHI:4701 2.00E-16 A6122_0156 IMP dehydrogenase Fdh3 Candida albicans mixed PHI:4903 8.00E-20 A6122_0158 acetyl-coenzyme A synthetase SidI Aspergillus fumigatus reduced virulence PHI:2321 9.00E-27 A6122_0160 ABC transporter substrate-binding protein ssuA Xanthomonas citri reduced virulence PHI:4028 9.00E-15 A6122_0161 ABC transporter pstB Xanthomonas citri loss of pathogenicity PHI:3412 4.00E-21 A6122_0163 dihydrolipoyl dehydrogenase Glr1 Candida albicans reduced virulence PHI:4904 2.00E-40 A6122_0166 threonine aldolase TOXG Cochliobolus carbonum reduced virulence PHI:469 0.000003 A6122_0168 DNA-binding response regulator degU Listeria monocytogenes reduced virulence PHI:4690 4.00E-46 A6122_0169 hypothetical protein hrpx Pantoea stewartii reduced virulence PHI:2473 0.000000006 A6122_0179 exonuclease crc Pseudomonas aeruginosa reduced virulence PHI:4900 5.00E-12 A6122_0181 multidrug ABC transporter ATP-binding protein ABC3 Magnaporthe oryzae loss of pathogenicity PHI:2042 1.00E-57 A6122_0182 orotate phosphoribosyltransferase URA5 Histoplasma capsulatum loss of pathogenicity PHI:159 5.00E-10 A6122_0212 bifunctional metallophosphatase/5'-nucleotidase S5nA Lactococcus lactis increased virulence (hypervirulence) PHI:5386 0.00000002 A6122_0213 adenylosuccinate synthase purA Salmonella enterica reduced virulence PHI:2625 5.00E-143 A6122_0216 peptide ABC transporter ATP-binding protein ABC3 Magnaporthe oryzae loss of pathogenicity PHI:2042 3.00E-21 A6122_0227 sugar ABC transporter ATPase ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 4.00E-15 A6122_0250 ABC transporter ATP-binding protein LmABCB3 Leishmania major reduced virulence PHI:5554 6.00E-18 A6122_0260 hypothetical protein rfbC Xanthomonas citri reduced virulence PHI:2481 1.00E-10 A6122_0263 hypothetical protein cps14B Streptococcus suis loss of pathogenicity PHI:5243 1.00E-10 A6122_0272 protein-tyrosine-phosphatase BCAL2200 Burkholderia cenocepacia reduced virulence PHI:5297 1.00E-32 A6122_0276 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha BCKDH Plasmodium falciparum reduced virulence PHI:3101 7.00E-40 A6122_0277 pyruvate dehydrogenase pdhB Mycoplasma agalactiae reduced virulence PHI:4610 8.00E-69 A6122_0280 LacI family transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 4.00E-34 A6122_0282 ABC transporter ATP-binding protein abcB Aspergillus fumigatus reduced virulence PHI:4230 2.00E-15 A6122_0284 peptide ABC transporter substrate-binding protein Fur Escherichia coli mixed PHI:4887 1.00E-14 A6122_0287 GntR family transcriptional regulator pdhR Yersinia pseudotuberculosis reduced virulence PHI:3135 0.000000002 A6122_0290 short-chain dehydrogenase BRM2 Alternaria alternata unaffected pathogenicity PHI:2844 5.00E-14 A6122_0296 two-component sensor histidine kinase trcS Mycobacterium tuberculosis increased virulence (hypervirulence) PHI:3617 2.00E-18 A6122_0297 DNA-binding response regulator PmrA Coxiella burnetii reduced virulence PHI:3599 5.00E-40 A6122_0301 1-pyrroline-5-carboxylate dehydrogenase Sdh1 Parastagonospora nodorum reduced virulence PHI:3914 5.00E-23 A6122_0302 LysR family transcriptional regulator vtlR Brucella abortus reduced virulence PHI:5012 6.00E-13 A6122_0307 polysaccharide deacetylase pgdA Listeria monocytogenes reduced virulence PHI:4689 0.000002 A6122_0317 pyruvate dehydrogenase spxB Streptococcus pneumoniae increased virulence (hypervirulence) PHI:3604 2.00E-54 A6122_0319 succinate-semialdehyde dehydrogenase Sdh1 Parastagonospora nodorum reduced virulence PHI:3914 2.00E-65 A6122_0320 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 4.00E-42 A6122_0330 glycogen debranching enzyme GlgX gbe1 Fusarium oxysporum unaffected pathogenicity PHI:4725 0.0000006 A6122_0331 hypothetical protein MGG_04556 Magnaporthe oryzae reduced virulence PHI:881 0.000000006 A6122_0334 hypothetical protein CspR Enterococcus faecalis reduced virulence PHI:2971 6.00E-22 A6122_0339 hypothetical protein PLSP1 Fusarium graminearum unaffected pathogenicity PHI:717 0.000006 A6122_0353 hypothetical protein stk Streptococcus suis reduced virulence PHI:4155 2.00E-42 A6122_0362 hypothetical protein hupA Edwardsiella tarda reduced virulence PHI:3074 0.000000003 A6122_0373 deoxycytidine triphosphate deaminase magA Klebsiella pneumoniae reduced virulence PHI:2652 2.00E-45 A6122_0382 hypothetical protein OxyR Pseudomonas syringae reduced virulence PHI:5305 6.00E-13 A6122_0385 alcohol dehydrogenase Xdh1 Parastagonospora nodorum unaffected pathogenicity PHI:2256 1.00E-11 A6122_0387 molecular chaperone DnaK LHS1 Magnaporthe oryzae reduced virulence PHI:2058 1.00E-29 A6122_0389 molecular chaperone DnaJ dnaJ Francisella tularensis unaffected pathogenicity PHI:4733 1.00E-28 A6122_0394 NTP pyrophosphohydrolase PSPTO_2696 Pseudomonas syringae unaffected pathogenicity PHI:3119 0.0000001 A6122_0403 ferrochelatase HEM15 Aspergillus fumigatus lethal PHI:2532 3.00E-16 A6122_0408 hypothetical protein XC_0892 Xanthomonas campestris reduced virulence PHI:3950 4.00E-124 A6122_0409 MFS transporter DEP3 Alternaria brassicicola mixed PHI:2377 4.00E-25 A6122_0412 hypothetical protein MGG_04556 Magnaporthe oryzae reduced virulence PHI:881 0.0000002 A6122_0414 hypothetical protein spr0084 Streptococcus pneumoniae reduced virulence PHI:3154 1.00E-38 A6122_0420 1-pyrroline-5-carboxylate dehydrogenase Sdh1 Parastagonospora nodorum reduced virulence PHI:3914 5.00E-26 A6122_0421 MarR family transcriptional regulator MprA Escherichia coli loss of pathogenicity PHI:5407 0.000008 A6122_0424 hypothetical protein rhr2 Candida albicans reduced virulence PHI:4992 7.00E-25 A6122_0427 multidrug MFS transporter BCMFS1 Botrytis cinerea unaffected pathogenicity PHI:544 3.00E-47 A6122_0434 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 3.00E-42 A6122_0437 Puromycin resistance protein pur8 BCMFS1 Botrytis cinerea unaffected pathogenicity PHI:544 5.00E-25 A6122_0440 hypothetical protein sirA Salmonella enterica reduced virulence PHI:560 7.00E-10 A6122_0441 hypothetical protein PhoQ Xanthomonas oryzae increased virulence (hypervirulence) PHI:1145 0.00000003 A6122_0444 GntR family transcriptional regulator Vatr2 Clavibacter michiganensis reduced virulence PHI:3028 5.00E-28 A6122_0447 chorismate mutase AroQ? Xanthomonas oryzae increased virulence (hypervirulence) PHI:2470 3.00E-23 A6122_0448 hydrogenase expression protein AcrD Erwinia amylovora unaffected pathogenicity PHI:4043 2.00E-77 A6122_0453 RNA helicase GzAra006 Fusarium graminearum unaffected pathogenicity PHI:1458 4.00E-53 A6122_0454 fasciclin MoFLP1 Magnaporthe oryzae reduced virulence PHI:4231 3.00E-10 A6122_0465 AAA family ATPase GzNF001 Fusarium graminearum unaffected pathogenicity PHI:1681 3.00E-30 A6122_0469 serine/threonine protein kinase stk Streptococcus suis reduced virulence PHI:4155 7.00E-22 A6122_0474 hypothetical protein chp-7 Clavibacter michiganensis effector (plant avirulence determinant) PHI:4931 7.00E-52 A6122_0475 hypothetical protein argD Erwinia amylovora mixed PHI:3126 7.00E-37 A6122_0476 hypothetical protein chp-7 Clavibacter michiganensis effector (plant avirulence determinant) PHI:4931 9.00E-52 A6122_0479 hypothetical protein pgaC Klebsiella pneumoniae reduced virulence PHI:3448 0.0000008 A6122_0483 hypothetical protein stk Streptococcus suis reduced virulence PHI:4155 1.00E-35 A6122_0501 4-aminobutyrate aminotransferase pvdH Pseudomonas aeruginosa unaffected pathogenicity PHI:5059 6.00E-56 A6122_0507 histidine kinase PhoQ Salmonella enterica unaffected pathogenicity PHI:4899 0.0000003 A6122_0508 two-component system response regulator zmpR Streptococcus pneumoniae unaffected pathogenicity PHI:3008 0.0000003 A6122_0512 acetyltransferase MMAR_0039 Mycobacterium marinum reduced virulence PHI:4675 4.00E-15 A6122_0515 LacI family transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 2.00E-33 A6122_0517 hypothetical protein MgMfs1 Zymoseptoria tritici chemistry target: sensitivity to chemical PHI:1161 2.00E-49 A6122_0526 RNA polymerase subunit sigma-70 rpoE Salmonella enterica reduced virulence PHI:2680 3.00E-19 A6122_0529 hypothetical protein orf408 Salmonella enterica reduced virulence PHI:612 2.00E-21 A6122_0530 aldo/keto reductase CSH1 Candida albicans reduced virulence PHI:419 5.00E-11 A6122_0532 phosphoglucomutase, alpha-D-glucose phosphate-specific PGM Streptococcus parauberis reduced virulence PHI:3325 1.00E-20 A6122_0535 hypothetical protein SidH Aspergillus fumigatus reduced virulence PHI:2322 1.00E-12 A6122_0540 hypothetical protein EF3245 Enterococcus faecalis unaffected pathogenicity PHI:2997 0.0000007 A6122_0542 DNA glycosylase Rv2924c Mycobacterium tuberculosis unaffected pathogenicity PHI:3627 0.000000004 A6122_0546 DEAD/DEAH box helicase GzAra006 Fusarium graminearum unaffected pathogenicity PHI:1458 3.00E-57 A6122_0547 short-chain dehydrogenase fabG1 Ralstonia solanacearum lethal PHI:5271 1.00E-21 A6122_0561 LuxR family transcriptional regulator narL Mycobacterium tuberculosis unaffected pathogenicity PHI:3620 0.00000001 A6122_0565 glutamine ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 9.00E-35 A6122_0568 30S ribosomal protein S12 GzOB039 Fusarium graminearum unaffected pathogenicity PHI:1599 1.00E-40 A6122_0574 DNA topoisomerase TOP1 Candida albicans reduced virulence PHI:80 0.000006 A6122_0579 50S ribosomal protein L2 GzOB009 Fusarium graminearum lethal PHI:1569 9.00E-56 A6122_0595 preprotein translocase subunit SecY secY Listeria monocytogenes increased virulence (hypervirulence) PHI:5257 1.00E-106 A6122_0603 PTS mannitol transporter subunit IIA ptsN Yersinia pseudotuberculosis reduced virulence PHI:3133 0.0000002 A6122_0604 mannitol-1-phosphate 5-dehydrogenase Mpd1 Parastagonospora nodorum unaffected pathogenicity PHI:2270 1.00E-124 A6122_0617 phosphoglucosamine mutase RfbB Vibrio cholerae reduced virulence PHI:703 4.00E-24 A6122_0619 glutamine-fructose-6-phosphate transaminase (isomerizing) GFA1 Aspergillus fumigatus mixed PHI:2513 6.00E-109 A6122_0620 holo-ACP synthase FAS2 Candida albicans loss of pathogenicity PHI:96 3.00E-10 A6122_0629 SAM-dependent methyltransferase MMAR_1664 Mycobacterium marinum reduced virulence PHI:3365 3.00E-33 A6122_0633 hypothetical protein SoxR Pantoea stewartii reduced virulence PHI:3092 3.00E-17 A6122_0635 isopropylmalate isomerase LYS4 Aspergillus fumigatus lethal PHI:2520 4.00E-39 A6122_0638 aspartate aminotransferase Vatr2 Clavibacter michiganensis reduced virulence PHI:3028 2.00E-19 A6122_0639 pyruvate carboxyltransferase MoLYS20 Magnaporthe oryzae reduced virulence PHI:3234 3.00E-31 A6122_0644 ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 4.00E-22 A6122_0645 ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 4.00E-18 A6122_0648 IMP dehydrogenase IMPDH Streptococcus suis reduced virulence PHI:4071 0 A6122_0649 inosine 5-monophosphate dehydrogenase IMPDH Streptococcus suis reduced virulence PHI:4071 6.00E-15 A6122_0651 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 5.00E-55 A6122_0652 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 3.00E-59 A6122_0667 long-chain-fatty-acid--CoA ligase SidI Aspergillus fumigatus reduced virulence PHI:2321 5.00E-56 A6122_0670 succinate--CoA ligase subunit alpha ACL1 Fusarium graminearum loss of pathogenicity PHI:2386 2.00E-11 A6122_0675 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 4.00E-59 A6122_0684 phosphoribosylglycinamide formyltransferase FMT Staphylococcus aureus reduced virulence PHI:3999 7.00E-14 A6122_0687 ABC transporter LmABCB3 Leishmania major reduced virulence PHI:5554 4.00E-49 A6122_0693 heat-shock protein Hsp20 hspX Mycobacterium tuberculosis increased virulence (hypervirulence) PHI:4496 1.00E-11 A6122_0696 formyltetrahydrofolate deformylase FMT Staphylococcus aureus reduced virulence PHI:3999 6.00E-11 A6122_0699 serine hydroxymethyltransferase glyA Edwardsiella ictaluri reduced virulence PHI:2962 5.00E-146 A6122_0701 alcohol dehydrogenase MoADH1 Magnaporthe oryzae unaffected pathogenicity PHI:5034 2.00E-39 A6122_0714 RNA polymerase subunit sigma ecf18 Pseudomonas syringae unaffected pathogenicity PHI:2878 1.00E-23 A6122_0716 phosphate ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 4.00E-96 A6122_0717 phosphate ABC transporter, permease protein PstA Rv0930 Mycobacterium tuberculosis unaffected pathogenicity PHI:3635 6.00E-69 A6122_0718 phosphate ABC transporter permease subunit PstC Rv0930 Mycobacterium tuberculosis unaffected pathogenicity PHI:3635 5.00E-11 A6122_0720 DNA mismatch repair protein MutT PSPTO_2696 Pseudomonas syringae unaffected pathogenicity PHI:3119 0.000005 A6122_0721 RNA degradosome polyphosphate kinase ppk Proteus mirabilis reduced virulence PHI:5063 9.00E-123 A6122_0723 transcriptional regulator SrrAB Staphylococcus aureus reduced virulence PHI:5466 4.00E-25 A6122_0732 histidine kinase barA Salmonella enterica unaffected pathogenicity PHI:562 1.00E-25 A6122_0733 XRE family transcriptional regulator SrrAB Staphylococcus aureus reduced virulence PHI:5466 3.00E-51 A6122_0736 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase XAC1722 Xanthomonas citri reduced virulence PHI:4145 5.00E-32 A6122_0740 sugar ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 2.00E-21 A6122_0741 hypothetical protein pknE Mycobacterium tuberculosis unaffected pathogenicity PHI:3625 0.0000003 A6122_0743 peptide methionine sulfoxide reductase msrB Staphylococcus aureus mixed PHI:4582 8.00E-27 A6122_0746 cold-shock protein CspR Enterococcus faecalis reduced virulence PHI:2971 4.00E-21 A6122_0747 molecular chaperone GroEL GroEL Porphyromonas gingivalis increased virulence (hypervirulence) PHI:3085 0 A6122_0748 histidine kinase PidS Burkholderia glumae reduced virulence PHI:2439 1.00E-22 A6122_0749 two-component system response regulator covR Streptococcus pyogenes reduced virulence PHI:4846 5.00E-54 A6122_0751 cold-shock protein CspR Enterococcus faecalis reduced virulence PHI:2971 3.00E-11 A6122_0759 phosphoketolase PA3613 Pseudomonas aeruginosa mixed PHI:4716 4.00E-24 A6122_0768 amidase iaaH-1 Pseudomonas savastanoi reduced virulence PHI:4171 5.00E-15 A6122_0770 hypothetical protein PptT Mycobacterium tuberculosis reduced virulence PHI:2629 1.00E-48 A6122_0772 hypothetical protein PePatK Penicillium expansum unaffected pathogenicity PHI:4505 4.00E-86 A6122_0773 non-ribosomal peptide synthetase pamA Paenibacillus larvae reduced virulence PHI:3090 0 A6122_0774 hypothetical protein PePatK Penicillium expansum unaffected pathogenicity PHI:4505 1.00E-91 A6122_0775 Long-chain-fatty-acid--CoA ligase Cif_B Pseudomonas cichorii reduced virulence PHI:4836 0 A6122_0776 non-ribosomal peptide synthetase Cif_B Pseudomonas cichorii reduced virulence PHI:4836 0 A6122_0778 polyketide beta-ketoacyl:ACP synthase KSA1 Fusarium graminearum unaffected pathogenicity PHI:730 7.00E-23 A6122_0780 hypothetical protein SidH Aspergillus fumigatus reduced virulence PHI:2322 3.00E-17 A6122_0781 polyketide synthase pamA Paenibacillus larvae reduced virulence PHI:3090 8.00E-29 A6122_0782 hypothetical protein OxyR Pseudomonas syringae reduced virulence PHI:5305 0.000003 A6122_0783 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 4.00E-36 A6122_0785 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 4.00E-59 A6122_0787 ATP-dependent RNA helicase HrpA GzOB019 Fusarium graminearum unaffected pathogenicity PHI:1579 4.00E-104 A6122_0790 hypothetical protein sseA Salmonella enterica reduced virulence PHI:616 3.00E-28 A6122_0795 O-acetylhomoserine aminocarboxypropyltransferase FVEG_12529 Fusarium verticillioides unaffected pathogenicity PHI:3385 1.00E-136 A6122_0797 hypothetical protein PMT6 Candida albicans reduced virulence PHI:452 0.00000003 A6122_0798 16S rRNA (cytidine(1402)-2'-O)-methyltransferase rsmI Staphylococcus aureus reduced virulence PHI:4960 6.00E-50 A6122_0801 deoxyribonuclease PsTatD4 Phytophthora sojae reduced virulence PHI:3071 1.00E-10 A6122_0802 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA Pectobacterium wasabiae increased virulence (hypervirulence) PHI:2735 2.00E-40 A6122_0804 oxidoreductase NIA1 Parastagonospora nodorum unaffected pathogenicity PHI:2336 2.00E-12 A6122_0805 ABC transporter ATP-binding protein ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 3.00E-10 A6122_0806 MarR family transcriptional regulator pecS Dickeya solani increased virulence (hypervirulence) PHI:4176 8.00E-28 A6122_0808 ribose-phosphate pyrophosphokinase FTT0673p/prsAp Francisella tularensis unaffected pathogenicity PHI:4732 1.00E-81 A6122_0818 nitrate/sulfonate/bicarbonate ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 2.00E-18 A6122_0825 NADH dehydrogenase Rv0392c Mycobacterium tuberculosis unaffected pathogenicity PHI:3629 5.00E-25 A6122_0833 amidase DUR1,2 Candida albicans reduced virulence PHI:2654 6.00E-13 A6122_0836 short-chain dehydrogenase MFP1 Magnaporthe oryzae reduced virulence PHI:2034 0.000001 A6122_0838 pyridine nucleotide-disulfide oxidoreductase Glr1 Candida albicans reduced virulence PHI:4904 1.00E-22 A6122_0840 enoyl-CoA hydratase SidH Aspergillus fumigatus reduced virulence PHI:2322 6.00E-26 A6122_0847 RNA helicase GzAra006 Fusarium graminearum unaffected pathogenicity PHI:1458 4.00E-68 A6122_0862 hypothetical protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 3.00E-18 A6122_0866 aminotransferase Vatr2 Clavibacter michiganensis reduced virulence PHI:3028 6.00E-11 A6122_0867 hypothetical protein CD3284 Clostridium difficile increased virulence (hypervirulence) PHI:4637 7.00E-48 A6122_0868 glycosyl transferase family 2 pgaC Klebsiella pneumoniae reduced virulence PHI:3448 0.000006 A6122_0877 sugar ABC transporter ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 0.0000001 A6122_0880 hypothetical protein ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 7.00E-24 A6122_0890 peptide chain release factor 2 MntE Streptococcus pyogenes unaffected pathogenicity PHI:4652 1.00E-37 A6122_0891 cell division ATP-binding protein FtsE pstB Xanthomonas citri loss of pathogenicity PHI:3412 2.00E-19 A6122_0893 SsrA-binding protein smpB Salmonella enterica reduced virulence PHI:2681 5.00E-35 A6122_0894 MFS transporter VdSge1 Verticillium dahliae loss of pathogenicity PHI:2731 2.00E-13 A6122_0900 aldo/keto reductase CSH1 Candida albicans reduced virulence PHI:419 5.00E-28 A6122_0907 trehalose-phosphate synthase TPS1 Candida albicans reduced virulence PHI:130 6.00E-95 A6122_0908 trehalose-phosphatase CaTPS2 Candida albicans reduced virulence PHI:242 0.0000001 A6122_0909 dihydroxy-acid dehydratase Ilv3A Aspergillus fumigatus reduced virulence PHI:2638 7.00E-159 A6122_0910 acetolactate synthase 1 catalytic subunit ILV2 Cryptococcus neoformans loss of pathogenicity PHI:358 1.00E-141 A6122_0911 acetolactate synthase small subunit MoIlv6 Magnaporthe oryzae loss of pathogenicity PHI:3976 1.00E-24 A6122_0924 hypothetical protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 6.00E-18 A6122_0926 ATP-binding protein ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 8.00E-25 A6122_0929 MFS transporter VdSge1 Verticillium dahliae loss of pathogenicity PHI:2731 4.00E-12 A6122_0930 3-isopropylmalate dehydrogenase LEU2 Saccharomyces cerevisiae reduced virulence PHI:504 1.00E-44 A6122_0931 branched-chain amino acid aminotransferase TOXF Cochliobolus carbonum loss of pathogenicity PHI:157 9.00E-37 A6122_0934 hypothetical protein GzAra006 Fusarium graminearum unaffected pathogenicity PHI:1458 5.00E-13 A6122_0937 cytidyltransferase rfaE Haemophilus parasuis reduced virulence PHI:3147 2.00E-77 A6122_0938 succinate-semialdehyde dehydrogenase Sdh1 Parastagonospora nodorum reduced virulence PHI:3914 3.00E-42 A6122_0941 short-chain dehydrogenase ABA4 Magnaporthe oryzae reduced virulence PHI:5440 4.00E-20 A6122_0942 D-beta-D-heptose 1-phosphate adenosyltransferase rfaE Haemophilus parasuis reduced virulence PHI:3147 3.00E-56 A6122_0944 glycosyl transferase bgsB Enterococcus faecalis reduced virulence PHI:4127 6.00E-13 A6122_0955 amino acid permease orf48 Salmonella enterica unaffected pathogenicity PHI:601 0.00000003 A6122_0957 NADPH:quinone oxidoreductase PKS1 Cochliobolus heterostrophus reduced virulence PHI:55 5.00E-30 A6122_0962 hypothetical protein GzAra007 Fusarium graminearum unaffected pathogenicity PHI:1459 5.00E-19 A6122_0966 peptidylprolyl isomerase FkpA Siccibacter turicensis reduced virulence PHI:4583 4.00E-11 A6122_0968 peptidase M4 VvpE Vibrio vulnificus reduced virulence PHI:3057 3.00E-17 A6122_0970 oxidoreductase BCFHG1 Botrytis cinerea unaffected pathogenicity PHI:2304 1.00E-15 A6122_0979 polyribonucleotide nucleotidyltransferase pnp Salmonella enterica reduced virulence PHI:3878 3.00E-180 A6122_0980 hypothetical protein CSH1 Candida albicans reduced virulence PHI:419 3.00E-13 A6122_0994 cell division protein FtsK EccC Streptomyces scabies unaffected pathogenicity PHI:2707 2.00E-17 A6122_0999 recombinase RecA recA Streptococcus pneumoniae reduced virulence PHI:4702 1.00E-145 A6122_1002 tRNA delta(2)-isopentenylpyrophosphate transferase GzC2H072 Fusarium graminearum unaffected pathogenicity PHI:1408 9.00E-18 A6122_1006 5,10-methylenetetrahydrofolate reductase MET13 Magnaporthe oryzae loss of pathogenicity PHI:3823 8.00E-37 A6122_1007 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase MET6 Magnaporthe oryzae loss of pathogenicity PHI:4688 0 A6122_1011 imidazoleglycerol-phosphate dehydratase PTH3 Magnaporthe oryzae loss of pathogenicity PHI:121 5.00E-41 A6122_1020 glutamate ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 2.00E-41 A6122_1026 N-acetyl-gamma-glutamyl-phosphate reductase MoARG5,6 Magnaporthe oryzae loss of pathogenicity PHI:5233 6.00E-29 A6122_1027 bifunctional ornithine acetyltransferase/N-acetylglutamate synthase MoARG7 Magnaporthe oryzae reduced virulence PHI:5234 1.00E-54 A6122_1028 acetylglutamate kinase argB Staphylococcus aureus unaffected pathogenicity PHI:2633 2.00E-26 A6122_1029 acetylornithine aminotransferase argD Erwinia amylovora mixed PHI:3126 2.00E-100 A6122_1030 ornithine carbamoyltransferase argF Staphylococcus aureus unaffected pathogenicity PHI:2634 6.00E-64 A6122_1031 argininosuccinate lyase argGH Streptococcus pneumoniae reduced virulence PHI:4922 2.00E-110 A6122_1042 chromosome partitioning protein Rv3213c Mycobacterium tuberculosis increased virulence (hypervirulence) PHI:3628 7.00E-64 A6122_1054 amino acid permease orf48 Salmonella enterica unaffected pathogenicity PHI:601 4.00E-21 A6122_1061 NADP-dependent oxidoreductase RED1 Cochliobolus heterostrophus reduced virulence PHI:2839 1.00E-56 A6122_1064 argininosuccinate synthase MoARG1 Magnaporthe oryzae loss of pathogenicity PHI:5232 4.00E-40 A6122_1066 thiosulfate sulfurtransferase sseA Salmonella enterica reduced virulence PHI:616 2.00E-30 A6122_1071 multidrug ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 4.00E-19 A6122_1075 acetyltransferase MMAR_0039 Mycobacterium marinum reduced virulence PHI:4675 0.000000001 A6122_1077 GntR family transcriptional regulator pdhR Yersinia pseudotuberculosis reduced virulence PHI:3135 0.0000008 A6122_1092 thioredoxin thioredoxin_1 Salmonella enterica reduced virulence PHI:2644 4.00E-19 A6122_1099 chromosome partitioning protein ParA Rv3213c Mycobacterium tuberculosis increased virulence (hypervirulence) PHI:3628 1.00E-16 A6122_1101 mycothione reductase Glr1 Candida albicans reduced virulence PHI:4904 7.00E-30 A6122_1103 long-chain-fatty-acid--CoA ligase SidI Aspergillus fumigatus reduced virulence PHI:2321 1.00E-30 A6122_1104 glucokinase GLKA Mycobacterium tuberculosis mixed PHI:2631 4.00E-27 A6122_1105 hypothetical protein PA2537 Pseudomonas aeruginosa reduced virulence PHI:3783 8.00E-15 A6122_1107 serine/threonine protein kinase stk Streptococcus suis reduced virulence PHI:4155 2.00E-72 A6122_1112 16S rRNA (cytosine(1402)-N(4))-methyltransferase rsmH Staphylococcus aureus reduced virulence PHI:4961 2.00E-68 A6122_1132 transcriptional regulator NrdR nrdR Streptococcus pyogenes mixed PHI:3464 3.00E-40 A6122_1133 dihydroorotate dehydrogenase (quinone) DHOD Trypanosoma cruzi lethal PHI:2596 0.000001 A6122_1137 cytochrome c oxidase subunit II XAC1258 Xanthomonas citri reduced virulence PHI:4147 9.00E-10 A6122_1140 ubiquinol-cytochrome c reductase cytochrome b subunit ubiquinol_oxidase Zymoseptoria tritici chemistry target: resistance to chemical PHI:835 1.00E-17 A6122_1161 DNA lyase Rv2924c Mycobacterium tuberculosis unaffected pathogenicity PHI:3627 7.00E-21 A6122_1171 GTP pyrophosphokinase spoT Pseudomonas syringae mixed PHI:4596 3.00E-173 A6122_1174 peptidylprolyl isomerase CPA1 Cryptococcus neoformans reduced virulence PHI:213 0.0000004 A6122_1175 AAA family ATPase GzRad003 Fusarium graminearum unaffected pathogenicity PHI:1685 6.00E-105 A6122_1184 3-dehydroquinate synthase aroB Burkholderia glumae reduced virulence PHI:4250 4.00E-66 A6122_1189 elongation factor P XC_2359 Xanthomonas campestris reduced virulence PHI:3953 3.00E-28 A6122_1192 aspartate carbamoyltransferase argF Staphylococcus aureus unaffected pathogenicity PHI:2634 3.00E-16 A6122_1201 methionine adenosyltransferase MGG_00383 Magnaporthe oryzae reduced virulence PHI:877 2.00E-129 A6122_1203 methionyl-tRNA formyltransferase FMT Staphylococcus aureus reduced virulence PHI:3999 2.00E-51 A6122_1204 methyltransferase rsmB Pectobacterium atrosepticum mixed PHI:2694 6.00E-34 A6122_1207 ATP phosphoribosyltransferase prt Salmonella enterica reduced virulence PHI:5109 3.00E-35 A6122_1213 tryptophan synthase subunit beta TRPS Aspergillus fumigatus lethal PHI:2517 3.00E-154 A6122_1214 tryptophan synthase subunit alpha TRPS Aspergillus fumigatus lethal PHI:2517 1.00E-42 A6122_1218 pyruvate kinase pykF Yersinia pseudotuberculosis reduced virulence PHI:3134 8.00E-111 A6122_1221 hypothetical protein SNF2 Candida albicans reduced virulence PHI:2821 5.00E-58 A6122_1229 transcriptional regulator degU Listeria monocytogenes reduced virulence PHI:4690 1.00E-16 A6122_1234 30S ribosomal protein S1 pnp Salmonella enterica reduced virulence PHI:3878 1.00E-12 A6122_1237 excinuclease ABC subunit A ABC3 Magnaporthe oryzae loss of pathogenicity PHI:2042 0.000007 A6122_1241 superoxide dismutase SOD2 Cryptococcus neoformans loss of pathogenicity PHI:410 5.00E-50 A6122_1247 6-phosphogluconolactonase CaNAG1 Candida albicans reduced virulence PHI:206 0.00000002 A6122_1258 Fe-S cluster assembly ATPase SufC ABC3 Magnaporthe oryzae loss of pathogenicity PHI:2042 3.00E-14 A6122_1261 ABC transporter ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 3.00E-10 A6122_1265 beta-ketoacyl-ACP reductase fabG1 Ralstonia solanacearum lethal PHI:5271 6.00E-60 A6122_1268 glycosyl transferase family 1 MgAlg2 Zymoseptoria tritici loss of pathogenicity PHI:2441 5.00E-11 A6122_1269 ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 5.00E-12 A6122_1273 trehalose synthase AGS1 Aspergillus fumigatus reduced virulence PHI:3902 0.000001 A6122_1275 mannosyltransferase gumH Xylella fastidiosa loss of pathogenicity PHI:2938 0.00000003 A6122_1277 NAD-dependent deacetylase cob Salmonella enterica unaffected pathogenicity PHI:5572 3.00E-16 A6122_1282 glycogen debranching enzyme GlgX gbe1 Fusarium oxysporum unaffected pathogenicity PHI:4725 0.0000001 A6122_1301 hypothetical protein FkpA Cronobacter universalis reduced virulence PHI:4584 9.00E-12 A6122_1308 apolipoprotein N-acyltransferase PA3984 Pseudomonas aeruginosa reduced virulence PHI:3782 6.00E-24 A6122_1313 short-chain dehydrogenase THR1 Colletotrichum lagenarium reduced virulence PHI:59 4.00E-14 A6122_1316 LacI family transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 1.00E-35 A6122_1325 hypothetical protein pbpX Listeria monocytogenes reduced virulence PHI:4692 8.00E-11 A6122_1343 hypothetical protein pbpX Listeria monocytogenes reduced virulence PHI:4692 8.00E-11 A6122_1349 ammonia channel protein mepB Colletotrichum gloeosporioides reduced virulence PHI:2710 3.00E-65 A6122_1351 thiosulfate sulfurtransferase sseA Salmonella enterica reduced virulence PHI:616 4.00E-21 A6122_1356 acetyl-CoA acetyltransferase Pot1p Candida albicans unaffected pathogenicity PHI:3687 1.00E-50 A6122_1359 aconitate hydratase LYS4 Aspergillus fumigatus lethal PHI:2520 5.00E-21 A6122_1367 DNA topoisomerase IV GyrA Burkholderia glumae chemistry target: sensitivity to chemical PHI:824 1.00E-152 A6122_1372 RNA polymerase sigma factor rpoS Salmonella enterica reduced virulence PHI:2683 3.00E-58 A6122_1373 MFS transporter AnsP Francisella tularensis reduced virulence PHI:3916 0.000000001 A6122_1376 dihydrolipoyl dehydrogenase Glr1 Candida albicans reduced virulence PHI:4904 3.00E-39 A6122_1386 polyphosphate glucokinase Rv2702 Mycobacterium tuberculosis unaffected pathogenicity PHI:3636 2.00E-76 A6122_1399 ribonuclease G RNase_E Salmonella enterica reduced virulence PHI:3723 9.00E-81 A6122_1401 nucleoside-diphosphate kinase XC_2203 Xanthomonas campestris reduced virulence PHI:3945 2.00E-32 A6122_1409 ATP-dependent protease ATP-binding subunit ClpX ClpX Staphylococcus aureus reduced virulence PHI:3004 0 A6122_1410 ATP-dependent Clp protease proteolytic subunit ClpP Salmonella enterica reduced virulence PHI:3040 2.00E-66 A6122_1411 ATP-dependent Clp protease proteolytic subunit ClpP Salmonella enterica reduced virulence PHI:3040 6.00E-67 A6122_1414 hypothetical protein GzZC087 Fusarium graminearum reduced virulence PHI:1772 0.0000004 A6122_1415 LuxR family transcriptional regulator degU Listeria monocytogenes reduced virulence PHI:4690 2.00E-21 A6122_1419 DNA glycosylase Rv2924c Mycobacterium tuberculosis unaffected pathogenicity PHI:3627 4.00E-11 A6122_1422 aminopeptidase N PepN Mycobacterium tuberculosis increased virulence (hypervirulence) PHI:3630 0 A6122_1424 methylisocitrate lyase Ss-oah1 Sclerotinia sclerotiorum reduced virulence PHI:3329 7.00E-36 A6122_1430 energy-dependent translational throttle protein EttA pstB Xanthomonas citri loss of pathogenicity PHI:3412 3.00E-11 A6122_1434 peptide methionine sulfoxide reductase msrA2 Staphylococcus aureus mixed PHI:4580 3.00E-41 A6122_1435 aldo/keto reductase CSH1 Candida albicans reduced virulence PHI:419 1.00E-18 A6122_1443 ABC transporter ATP-binding protein ABC3 Magnaporthe oryzae loss of pathogenicity PHI:2042 6.00E-22 A6122_1448 DNA polymerase IV GzC2H071 Fusarium graminearum lethal PHI:1407 2.00E-20 A6122_1451 long-chain-fatty-acid--CoA ligase SidI Aspergillus fumigatus reduced virulence PHI:2321 2.00E-63 A6122_1456 aspartyl/glutamyl-tRNA amidotransferase subunit A iaaH-1 Pseudomonas savastanoi reduced virulence PHI:4171 3.00E-30 A6122_1462 glycosyl hydrolase gbe1 Fusarium oxysporum unaffected pathogenicity PHI:4725 0.000006 A6122_1464 glycogen phosphorylase GPH1 Magnaporthe oryzae reduced virulence PHI:3815 4.00E-16 A6122_1466 1,4-alpha-glucan branching enzyme gbe1 Fusarium oxysporum unaffected pathogenicity PHI:4725 2.00E-29 A6122_1475 phosphatidate cytidylyltransferase CDS1 Aspergillus fumigatus lethal PHI:2539 5.00E-13 A6122_1477 UMP kinase FVEG_12521 Fusarium verticillioides unaffected pathogenicity PHI:3389 0.000004 A6122_1480 MFS transporter MoST1 Magnaporthe oryzae unaffected pathogenicity PHI:4024 1.00E-52 A6122_1482 hypothetical protein cnpB Mycobacterium tuberculosis reduced virulence PHI:4238 3.00E-81 A6122_1505 chromosome segregation protein SMC GzHMG032 Fusarium graminearum unaffected pathogenicity PHI:1500 2.00E-17 A6122_1506 hypothetical protein Moatg7 Magnaporthe oryzae loss of pathogenicity PHI:2075 0.0000007 A6122_1521 hydroxyisourate hydrolase yedX Salmonella enterica unaffected pathogenicity PHI:2651 7.00E-31 A6122_1529 hypothetical protein DUR1,2 Candida albicans reduced virulence PHI:2654 9.00E-16 A6122_1531 DNA-formamidopyrimidine glycosylase Rv2924c Mycobacterium tuberculosis unaffected pathogenicity PHI:3627 3.00E-78 A6122_1532 ribonuclease III RNase_III Salmonella enterica reduced virulence PHI:3724 2.00E-38 A6122_1542 hypothetical protein OxyR Pseudomonas syringae reduced virulence PHI:5305 0.0000003 A6122_1543 peroxiredoxin TSA1 Cryptococcus neoformans reduced virulence PHI:386 3.00E-15 A6122_1546 ACP S-malonyltransferase F-avi4330 Agrobacterium vitis loss of pathogenicity PHI:2895 2.00E-11 A6122_1549 beta-ketoacyl-[acyl-carrier-protein] synthase II KasB Mycobacterium tuberculosis reduced virulence PHI:4196 3.00E-112 A6122_1555 disulfide bond formation protein DsbA BCMFS1 Botrytis cinerea unaffected pathogenicity PHI:544 7.00E-39 A6122_1556 hypothetical protein hasA Streptococcus pyogenes unaffected pathogenicity PHI:5264 0.000007 A6122_1562 glucosyl transferase bgsB Enterococcus faecalis reduced virulence PHI:4127 4.00E-22 A6122_1563 multidrug DMT transporter permease orf19.5352 Candida albicans unaffected pathogenicity PHI:4891 0.000001 A6122_1570 glutathione ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 6.00E-26 A6122_1572 peptide ABC transporter permease App Clostridium difficile increased virulence (hypervirulence) PHI:4612 5.00E-47 A6122_1584 2-isopropylmalate synthase MoLYS20 Magnaporthe oryzae reduced virulence PHI:3234 0.000005 A6122_1585 LuxR family transcriptional regulator degU Listeria monocytogenes reduced virulence PHI:4690 7.00E-40 A6122_1589 rRNA maturation RNase YbeY YbeY Vibrio cholerae loss of pathogenicity PHI:3059 3.00E-14 A6122_1593 molecular chaperone DnaJ mas5 Beauveria bassiana loss of pathogenicity PHI:5394 3.00E-34 A6122_1608 hypothetical protein pabaA Aspergillus fumigatus reduced virulence PHI:178 5.00E-47 A6122_1612 thiol reductant ABC exporter subunit CydC LmABCB3 Leishmania major reduced virulence PHI:5554 4.00E-26 A6122_1613 thiol reductant ABC exporter subunit CydD LmABCB3 Leishmania major reduced virulence PHI:5554 9.00E-21 A6122_1623 ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 3.00E-23 A6122_1626 short-chain dehydrogenase/reductase fabG1 Ralstonia solanacearum lethal PHI:5271 1.00E-40 A6122_1628 enoyl-acyl carrier protein reductases patN Penicillium expansum unaffected pathogenicity PHI:3301 0.000008 A6122_1634 polyisoprenoid-binding protein PA0423 Pseudomonas aeruginosa reduced virulence PHI:3166 2.00E-30 A6122_1635 MFS transporter BCMFS1 Botrytis cinerea unaffected pathogenicity PHI:544 4.00E-13 A6122_1641 ATP-dependent DNA helicase RecG GzAra006 Fusarium graminearum unaffected pathogenicity PHI:1458 0.0000004 A6122_1652 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 4.00E-59 A6122_1653 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 8.00E-49 A6122_1657 3-isopropylmalate dehydratase large subunit LYS4 Aspergillus fumigatus lethal PHI:2520 8.00E-37 A6122_1666 3',5'-cyclic-nucleotide phosphodiesterase cnpB Mycobacterium tuberculosis reduced virulence PHI:4238 2.00E-126 A6122_1669 protease 2 Oligopeptidase_B Trypanosoma cruzi reduced virulence PHI:2601 3.00E-153 A6122_1672 transporter corA Pectobacterium carotovorum reduced virulence PHI:2479 8.00E-12 A6122_1678 helicase GzMyb016 Fusarium graminearum unaffected pathogenicity PHI:1552 1.00E-28 A6122_1680 hypothetical protein RicR Mycobacterium tuberculosis reduced virulence PHI:4070 5.00E-11 A6122_1684 NAD(P)-dependent oxidoreductase AS87_04050 Riemerella anatipestifer reduced virulence PHI:4819 0.000002 A6122_1701 hypothetical protein FkpA Cronobacter universalis reduced virulence PHI:4584 0.000000004 A6122_1702 4-aminobutyrate aminotransferase argD Erwinia amylovora mixed PHI:3126 4.00E-59 A6122_1703 acyl-phosphate glycerol 3-phosphate acyltransferase PA2537 Pseudomonas aeruginosa reduced virulence PHI:3783 0.0000001 A6122_1705 4-aminobutyrate aminotransferase pvdH Pseudomonas aeruginosa unaffected pathogenicity PHI:5059 1.00E-54 A6122_1709 spermidine/putrescine ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 9.00E-27 A6122_1714 hypothetical protein argD Erwinia amylovora mixed PHI:3126 4.00E-45 A6122_1716 cysteine methyltransferase GzWing026 Fusarium graminearum unaffected pathogenicity PHI:1653 0.00000003 A6122_1720 F0F1 ATP synthase subunit beta invC Salmonella enterica reduced virulence PHI:645 1.00E-39 A6122_1722 F0F1 ATP synthase subunit alpha bscN Bordetella bronchiseptica reduced virulence PHI:3411 1.00E-32 A6122_1728 UDP-N-acetylmuramyl pentapeptide phosphotransferase ALG7 Aspergillus fumigatus lethal PHI:2515 0.00000004 A6122_1733 hypothetical protein MgAlg2 Zymoseptoria tritici loss of pathogenicity PHI:2441 7.00E-10 A6122_1738 glucose-1-phosphate thymidylyltransferase galU Xanthomonas campestris loss of pathogenicity PHI:3112 6.00E-14 A6122_1739 dTDP-glucose 4,6-dehydratase AS87_04050 Riemerella anatipestifer reduced virulence PHI:4819 3.00E-24 A6122_1750 peptide chain release factor 1 MntE Streptococcus pyogenes unaffected pathogenicity PHI:4652 2.00E-95 A6122_1751 transcription termination factor Rho ssaN Salmonella enterica reduced virulence PHI:624 5.00E-19 A6122_1755 diaminopimelate decarboxylase ODC Parastagonospora nodorum reduced virulence PHI:177 5.00E-11 A6122_1762 two-component system response regulator covR Streptococcus pyogenes reduced virulence PHI:4846 4.00E-43 A6122_1763 hypothetical protein qseC Pectobacterium carotovorum reduced virulence PHI:3709 9.00E-21 A6122_1781 hypothetical protein pknE Mycobacterium tuberculosis unaffected pathogenicity PHI:3625 0.00000001 A6122_1783 ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 1.00E-23 A6122_1785 ABC transporter permease App Clostridium difficile increased virulence (hypervirulence) PHI:4612 5.00E-36 A6122_1789 glycine dehydrogenase (aminomethyl-transferring) gcvP Edwardsiella ictaluri reduced virulence PHI:2961 0 A6122_1792 ADP-ribose pyrophosphatase PA4916 Pseudomonas aeruginosa reduced virulence PHI:4718 1.00E-14 A6122_1794 L-aspartate oxidase frdA Edwardsiella ictaluri reduced virulence PHI:2960 2.00E-60 A6122_1796 cysteine desulfurase nifS Mycoplasma agalactiae loss of pathogenicity PHI:4210 2.00E-13 A6122_1797 MFS transporter VdSge1 Verticillium dahliae loss of pathogenicity PHI:2731 3.00E-22 A6122_1798 amino acid adenylation protein F-avi3342 Agrobacterium vitis loss of pathogenicity PHI:2894 7.00E-73 A6122_1801 polyphosphate kinase 2 FTT1564 Francisella tularensis unaffected pathogenicity PHI:5415 8.00E-120 A6122_1802 hypothetical protein THR1 Colletotrichum lagenarium reduced virulence PHI:59 7.00E-10 A6122_1825 carbonic anhydrase Can2 Cryptococcus neoformans mixed PHI:674 5.00E-10 A6122_1826 aspartate ammonia-lyase ARG1 Fusarium oxysporum reduced virulence PHI:200 0.00000007 A6122_1837 threonine ammonia-lyase MoIlv1 Magnaporthe oryzae reduced virulence PHI:3257 3.00E-47 A6122_1839 spermidine/putrescine ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 2.00E-29 A6122_1856 phosphoribosyltransferase XhpT Xylella fastidiosa reduced virulence PHI:2936 0.000005 A6122_1857 oxidoreductase fabG1 Ralstonia solanacearum lethal PHI:5271 2.00E-13 A6122_1859 bifunctional o-acetylhomoserine/o-acetylserine sulfhydrylase FVEG_12529 Fusarium verticillioides unaffected pathogenicity PHI:3385 8.00E-136 A6122_1860 homoserine O-acetyltransferase FVEG_12519 Fusarium verticillioides unaffected pathogenicity PHI:3391 1.00E-56 A6122_1863 LuxR family transcriptional regulator narL Mycobacterium tuberculosis unaffected pathogenicity PHI:3620 2.00E-12 A6122_1864 alcohol dehydrogenase Xdh1 Parastagonospora nodorum unaffected pathogenicity PHI:2256 2.00E-26 A6122_1868 hypothetical protein BCFHG1 Botrytis cinerea unaffected pathogenicity PHI:2304 0.0000003 A6122_1874 transporter corA Pectobacterium carotovorum reduced virulence PHI:2479 4.00E-14 A6122_1875 inosine 5-monophosphate dehydrogenase IMPDH Streptococcus suis reduced virulence PHI:4071 2.00E-67 A6122_1878 hypothetical protein rpoE Salmonella enterica reduced virulence PHI:2680 3.00E-22 A6122_1879 3-phosphoshikimate 1-carboxyvinyltransferase aroA Salmonella enterica reduced virulence PHI:2624 6.00E-43 A6122_1881 peroxiredoxin TPX1 Magnaporthe oryzae reduced virulence PHI:5191 3.00E-15 A6122_1884 cell division protein FtsK EccC Streptomyces scabies unaffected pathogenicity PHI:2707 4.00E-58 A6122_1886 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 5.00E-59 A6122_1890 serine/threonine protein kinase stk Streptococcus suis reduced virulence PHI:4155 7.00E-36 A6122_1891 alcohol dehydrogenase CSH1 Candida albicans reduced virulence PHI:419 2.00E-66 A6122_1893 WhiB family transcriptional regulator H37Rv Mycobacterium tuberculosis reduced virulence PHI:5575 4.00E-14 A6122_1894 ATPase Rv3220c Mycobacterium tuberculosis unaffected pathogenicity PHI:3621 2.00E-111 A6122_1896 preprotein translocase subunit SecA secA Acinetobacter baumannii reduced virulence PHI:5240 0 A6122_1897 RNA polymerase subunit sigma-54 yhbH Erwinia amylovora loss of pathogenicity PHI:3680 0.0000007 A6122_1900 histidine kinase ssrA Salmonella enterica reduced virulence PHI:2676 7.00E-27 A6122_1901 two-component system response regulator SrrAB Staphylococcus aureus reduced virulence PHI:5466 8.00E-55 A6122_1913 transcription factor WhiB H37Rv Mycobacterium tuberculosis reduced virulence PHI:5575 4.00E-10 A6122_1914 UDP-glucose 4-epimerase GalE TcGALE Trypanosoma cruzi lethal PHI:2589 6.00E-63 A6122_1915 mannose-6-phosphate isomerase, class I MAN1 Cryptococcus neoformans reduced virulence PHI:220 2.00E-36 A6122_1920 GDP-mannose 4,6-dehydratase AS87_04050 Riemerella anatipestifer reduced virulence PHI:4819 4.00E-13 A6122_1921 GDP-mannose 4,6-dehydratase AS87_04050 Riemerella anatipestifer reduced virulence PHI:4819 8.00E-11 A6122_1926 hypothetical protein ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 2.00E-12 A6122_1929 glycosyl transferase pgaC Klebsiella pneumoniae reduced virulence PHI:3448 2.00E-12 A6122_1934 hypothetical protein rfbC Xanthomonas citri reduced virulence PHI:2481 1.00E-11 A6122_1936 NAD(P)-dependent oxidoreductase GacA Streptococcus pyogenes lethal PHI:5069 1.00E-53 A6122_1940 transcriptional regulator EF3245 Enterococcus faecalis unaffected pathogenicity PHI:2997 0.000000009 A6122_1942 5-(carboxyamino)imidazole ribonucleotide synthase ADE2 Saccharomyces cerevisiae reduced virulence PHI:501 3.00E-55 A6122_1950 LuxR family transcriptional regulator narL Mycobacterium tuberculosis unaffected pathogenicity PHI:3620 2.00E-10 A6122_1953 acetyl-/propionyl-CoA carboxylase subunit alpha DUR1,2 Candida albicans reduced virulence PHI:2654 4.00E-100 A6122_1954 flavoprotein disulfide reductase Glr1 Candida albicans reduced virulence PHI:4904 2.00E-17 A6122_1956 phosphomannomutase PGM Streptococcus parauberis reduced virulence PHI:3325 4.00E-87 A6122_1964 heme ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 6.00E-17 A6122_1967 hypothetical protein bgsB Enterococcus faecalis reduced virulence PHI:4127 4.00E-13 A6122_1970 succinate dehydrogenase flavoprotein subunit sdi1 Zymoseptoria tritici unaffected pathogenicity PHI:4966 0 A6122_1971 succinate dehydrogenase iron-sulfur subunit Ip Zymoseptoria tritici chemistry target: resistance to chemical PHI:822 6.00E-61 A6122_1974 D-beta-D-heptose 1-phosphate adenosyltransferase orf408 Salmonella enterica reduced virulence PHI:612 0.000002 A6122_1976 PTS fructose transporter subunit IIA ptsN Yersinia pseudotuberculosis reduced virulence PHI:3133 0.000000007 A6122_1978 exodeoxyribonuclease III crc Pseudomonas aeruginosa reduced virulence PHI:4900 2.00E-40 A6122_1983 FAD-dependent oxidoreductase glpD Yersinia pestis unaffected pathogenicity PHI:3272 3.00E-41 A6122_1991 beta-fructofuranosidase lsc Erwinia amylovora reduced virulence PHI:2472 2.00E-74 A6122_1998 long-chain-fatty-acid--CoA ligase SidI Aspergillus fumigatus reduced virulence PHI:2321 5.00E-59 A6122_1999 hypothetical protein FVEG_12523 Fusarium verticillioides unaffected pathogenicity PHI:3387 1.00E-13 A6122_2000 acetyl-CoA acetyltransferase ERG10 Aspergillus fumigatus lethal PHI:2529 7.00E-86 A6122_2002 hypothetical protein ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 5.00E-17 A6122_2010 hypothetical protein FVEG_12529 Fusarium verticillioides unaffected pathogenicity PHI:3385 2.00E-49 A6122_2011 hypothetical protein SID1 Colletotrichum graminicola reduced virulence PHI:4216 1.00E-55 A6122_2013 hypothetical protein Rv3597c Mycobacterium tuberculosis unaffected pathogenicity PHI:3632 0.00000001 A6122_2016 UTP--glucose-1-phosphate uridylyltransferase galU Xanthomonas campestris loss of pathogenicity PHI:3112 3.00E-75 A6122_2028 RNA polymerase subunit sigma ecf18 Pseudomonas syringae unaffected pathogenicity PHI:2878 6.00E-15 A6122_2038 4-hydroxyacetophenone monooxygenase DEP4 Alternaria brassicicola mixed PHI:2378 7.00E-12 A6122_2040 MarR family transcriptional regulator pecS Dickeya solani increased virulence (hypervirulence) PHI:4176 1.00E-14 A6122_2049 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 1.00E-33 A6122_2054 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 5.00E-42 A6122_2061 hypothetical protein Rv3597c Mycobacterium tuberculosis unaffected pathogenicity PHI:3632 2.00E-16 A6122_2063 molybdopterin biosynthesis protein MoeZ Moatg7 Magnaporthe oryzae loss of pathogenicity PHI:2075 0.0000007 A6122_2076 hypothetical protein Rv3597c Mycobacterium tuberculosis unaffected pathogenicity PHI:3632 6.00E-20 A6122_2093 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 5.00E-42 A6122_2095 GntR family transcriptional regulator agaR1 Streptococcus suis unaffected pathogenicity PHI:5301 0.000005 A6122_2096 ABC transporter ATP-binding protein ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 3.00E-15 A6122_2098 integrase hopA1 Pseudomonas cichorii mixed PHI:3165 0.000000008 A6122_2099 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 5.00E-58 A6122_2103 oxidoreductase CSH1 Candida albicans reduced virulence PHI:419 0.00000007 A6122_2107 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 4.00E-59 A6122_2113 cytosine methyltransferase Rv2924c Mycobacterium tuberculosis unaffected pathogenicity PHI:3627 1.00E-11 A6122_2116 ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 5.00E-16 A6122_2128 MFS transporter GIT3 Candida albicans reduced virulence PHI:3822 0.00000006 A6122_2129 ABC transporter ABC3 Magnaporthe oryzae loss of pathogenicity PHI:2042 9.00E-51 A6122_2130 3-hydroxy-2-methylbutyryl-CoA dehydrogenase ABA4 Magnaporthe oryzae reduced virulence PHI:5440 7.00E-17 A6122_2133 NAD-dependent succinate-semialdehyde dehydrogenase MoSSADH Magnaporthe oryzae loss of pathogenicity PHI:2145 4.00E-129 A6122_2146 hypothetical protein tadA Pectobacterium atrosepticum reduced virulence PHI:3843 8.00E-59 A6122_2147 acetyl-coenzyme A synthetase dhbF Paenibacillus larvae unaffected pathogenicity PHI:3274 4.00E-30 A6122_2153 UDP-glucose 6-dehydrogenase UGD1 Cryptococcus neoformans loss of pathogenicity PHI:387 6.00E-65 A6122_2154 hypothetical protein BceD Burkholderia contaminans reduced virulence PHI:5025 9.00E-11 A6122_2155 hypothetical protein BceF Burkholderia contaminans reduced virulence PHI:5026 6.00E-46 A6122_2157 polyprenyl glycosylphosphotransferase gumD Xylella fastidiosa loss of pathogenicity PHI:2937 3.00E-35 A6122_2163 aspartate kinase FVEG_12521 Fusarium verticillioides unaffected pathogenicity PHI:3389 1.00E-21 A6122_2168 formaldehyde dehydrogenase, glutathione-independent Xdh1 Parastagonospora nodorum unaffected pathogenicity PHI:2256 2.00E-18 A6122_2183 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 3.00E-42 A6122_2184 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 3.00E-42 A6122_2190 hypothetical protein GzCCCH001 Fusarium graminearum unaffected pathogenicity PHI:1657 9.00E-10 A6122_2191 hypothetical protein MET13 Magnaporthe oryzae loss of pathogenicity PHI:3823 0.0000001 A6122_2193 transcriptional regulator GLKA Mycobacterium tuberculosis mixed PHI:2631 0.000003 A6122_2194 alpha-xylosidase Gas1 Ustilago maydis loss of pathogenicity PHI:1071 9.00E-27 A6122_2198 hypothetical protein CcpA Enterococcus faecium reduced virulence PHI:4564 2.00E-16 A6122_2203 glycine/betaine ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 8.00E-22 A6122_2206 hypothetical protein chp-7 Clavibacter michiganensis effector (plant avirulence determinant) PHI:4931 1.00E-12 A6122_2207 hypothetical protein chp-7 Clavibacter michiganensis effector (plant avirulence determinant) PHI:4931 9.00E-28 A6122_2208 hypothetical protein chp-7 Clavibacter michiganensis effector (plant avirulence determinant) PHI:4931 1.00E-27 A6122_2216 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 3.00E-42 A6122_2229 ferredoxin YHB1 Candida albicans reduced virulence PHI:500 1.00E-14 A6122_2235 LacI family transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 1.00E-36 A6122_2237 ABC transporter ATP-binding protein ABC3 Magnaporthe oryzae loss of pathogenicity PHI:2042 6.00E-14 A6122_2247 hypothetical protein stk Streptococcus suis reduced virulence PHI:4155 0.0000008 A6122_2249 AAA family ATPase clpV?5 Burkholderia pseudomallei effector (plant avirulence determinant) PHI:5335 1.00E-62 A6122_2250 aldo/keto reductase CSH1 Candida albicans reduced virulence PHI:419 5.00E-13 A6122_2259 macrolide export ATP-binding/permease protein MacB pstB Xanthomonas citri loss of pathogenicity PHI:3412 3.00E-22 A6122_2264 cystathionine gamma-synthase CBL1 Fusarium graminearum reduced virulence PHI:443 2.00E-81 A6122_2270 ABC transporter LmABCB3 Leishmania major reduced virulence PHI:5554 1.00E-68 A6122_2274 LacI family transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 5.00E-20 A6122_2275 hypothetical protein MMAR_0039 Mycobacterium marinum reduced virulence PHI:4675 6.00E-15 A6122_2287 UDP-glucose 4-epimerase Uge3 Aspergillus nidulans increased virulence (hypervirulence) PHI:5268 2.00E-36 A6122_2292 hypothetical protein trcS Mycobacterium tuberculosis increased virulence (hypervirulence) PHI:3617 3.00E-76 A6122_2293 two-component system response regulator covR Streptococcus pyogenes reduced virulence PHI:4846 3.00E-56 A6122_2295 amino acid permease orf48 Salmonella enterica unaffected pathogenicity PHI:601 0.00000007 A6122_2303 hypothetical protein GLKA Mycobacterium tuberculosis mixed PHI:2631 0.000006 A6122_2306 NADPH:quinone reductase F-avi4330 Agrobacterium vitis loss of pathogenicity PHI:2895 5.00E-14 A6122_2312 aspartate aminotransferase Vatr2 Clavibacter michiganensis reduced virulence PHI:3028 9.00E-11 A6122_2313 ABC transporter ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 4.00E-29 A6122_2316 acyl dehydratase TOXC Cochliobolus carbonum reduced virulence PHI:97 0.0000002 A6122_2317 hypothetical protein hadC Mycobacterium tuberculosis reduced virulence PHI:5351 4.00E-21 A6122_2322 hypothetical protein pchA Pseudomonas aeruginosa unaffected pathogenicity PHI:5057 2.00E-10 A6122_2324 hypothetical protein CcpA Enterococcus faecium reduced virulence PHI:4564 1.00E-22 A6122_2332 alcohol dehydrogenase CSH1 Candida albicans reduced virulence PHI:419 3.00E-25 A6122_2341 hypothetical protein ypo2062 Yersinia pestis reduced virulence PHI:4185 3.00E-10 A6122_2344 YajQ family cyclic di-GMP-binding protein XC_3703 Xanthomonas campestris mixed PHI:3259 5.00E-32 A6122_2346 daunorubicin resistance protein DrrC pstB Xanthomonas citri loss of pathogenicity PHI:3412 0.0000004 A6122_2349 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase MMAR_1663 Mycobacterium marinum reduced virulence PHI:3364 9.00E-11 A6122_2350 isochorismate synthase pchA Pseudomonas aeruginosa unaffected pathogenicity PHI:5057 2.00E-44 A6122_2357 1,4-dihydroxy-2-naphthoyl-CoA synthase SidH Aspergillus fumigatus reduced virulence PHI:2322 7.00E-20 A6122_2359 ketoacyl reductase ABA4 Magnaporthe oryzae reduced virulence PHI:5440 8.00E-25 A6122_2369 short-chain dehydrogenase entA Bacillus cereus reduced virulence PHI:4077 6.00E-16 A6122_2370 aspartate--tRNA(Asn) ligase GzOB012 Fusarium graminearum unaffected pathogenicity PHI:1572 6.00E-62 A6122_2372 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 4.00E-59 A6122_2384 transcriptional regulator SrrAB Staphylococcus aureus reduced virulence PHI:5466 2.00E-12 A6122_2385 transcriptional regulator RovA Yersinia pestis reduced virulence PHI:6114 0.000000004 A6122_2386 deoxyribodipyrimidine photo-lyase FTT0846 Francisella tularensis reduced virulence PHI:4730 2.00E-33 A6122_2387 serine O-acetyltransferase lacA Salmonella enterica reduced virulence PHI:3436 0.00000003 A6122_2389 hypothetical protein stk Streptococcus suis reduced virulence PHI:4155 0.0000001 A6122_2391 hypothetical protein GzMyb016 Fusarium graminearum unaffected pathogenicity PHI:1552 6.00E-68 A6122_2392 hypothetical protein MgAlg2 Zymoseptoria tritici loss of pathogenicity PHI:2441 0.000005 A6122_2393 polyphosphate glucokinase PPGK Mycobacterium tuberculosis mixed PHI:2630 1.00E-50 A6122_2396 nucleoside-diphosphate sugar epimerase AS87_04050 Riemerella anatipestifer reduced virulence PHI:4819 0.00000004 A6122_2397 hydrolase SidI Aspergillus fumigatus reduced virulence PHI:2321 7.00E-10 A6122_2403 carbonate dehydratase copA Vibrio tasmaniensis reduced virulence PHI:5275 2.00E-128 A6122_2413 hypothetical protein stk Streptococcus suis reduced virulence PHI:4155 0.000006 A6122_2414 MarR family transcriptional regulator MprA Escherichia coli loss of pathogenicity PHI:5407 0.000000001 A6122_2433 hypothetical protein vtlR Brucella abortus reduced virulence PHI:5012 4.00E-11 A6122_2443 NDP-hexose 4-ketoreductase clpV?5 Burkholderia pseudomallei effector (plant avirulence determinant) PHI:5335 9.00E-76 A6122_2445 hypothetical protein EF3245 Enterococcus faecalis unaffected pathogenicity PHI:2997 0.000001 A6122_2447 pyridine nucleotide-disulfide oxidoreductase Rv0392c Mycobacterium tuberculosis unaffected pathogenicity PHI:3629 1.00E-30 A6122_2449 cardiolipin synthase PLD2 Acinetobacter baumannii reduced virulence PHI:5093 1.00E-11 A6122_2452 ABC transporter-like protein ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 2.00E-38 A6122_2458 lysine--tRNA ligase GzOB034 Fusarium graminearum unaffected pathogenicity PHI:1594 3.00E-96 A6122_2459 pantoate--beta-alanine ligase Pbl1 Parastagonospora nodorum unaffected pathogenicity PHI:2257 7.00E-41 A6122_2462 ABC transporter ATP-binding protein ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 3.00E-23 A6122_2463 DNA-binding response regulator degU Listeria monocytogenes reduced virulence PHI:4690 7.00E-42 A6122_2475 cell division protein FtsH GzOB006 Fusarium graminearum lethal PHI:1566 2.00E-59 A6122_2476 hypothetical protein bpaC Burkholderia pseudomallei reduced virulence PHI:4668 7.00E-18 A6122_2479 hypoxanthine phosphoribosyltransferase XhpT Xylella fastidiosa reduced virulence PHI:2936 3.00E-14 A6122_2481 inorganic pyrophosphatase IPP1 Aspergillus fumigatus lethal PHI:2540 0.0000002 A6122_2483 hypothetical protein ypo2062 Yersinia pestis reduced virulence PHI:4185 5.00E-16 A6122_2485 GTP pyrophosphokinase RelBbu Borrelia burgdorferi mixed PHI:5102 8.00E-10 A6122_2487 MFS transporter permease MgMfs1 Zymoseptoria tritici chemistry target: sensitivity to chemical PHI:1161 7.00E-11 A6122_2490 hypothetical protein ohrR Vibrio cholerae reduced virulence PHI:5549 0.000004 A6122_2491 S-(hydroxymethyl)mycothiol dehydrogenase MoSFA1 Magnaporthe oryzae reduced virulence PHI:4616 9.00E-54 A6122_2498 hypothetical protein CcpA Enterococcus faecium reduced virulence PHI:4564 4.00E-10 A6122_2499 malate dehydrogenase mdh Edwardsiella ictaluri reduced virulence PHI:2959 0.00000004 A6122_2501 ATP-dependent DNA helicase RecQ GzAra006 Fusarium graminearum unaffected pathogenicity PHI:1458 0.0000001 A6122_2504 ABC transporter ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 3.00E-18 A6122_2510 ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 6.00E-19 A6122_2512 ABC transporter permease App Clostridium difficile increased virulence (hypervirulence) PHI:4612 9.00E-40 A6122_2516 LuxR family transcriptional regulator degU Listeria monocytogenes reduced virulence PHI:4690 6.00E-16 A6122_2520 hypothetical protein CcpA Enterococcus faecium reduced virulence PHI:4564 4.00E-28 A6122_2534 PadR family transcriptional regulator AphA Vibrio cholerae reduced virulence PHI:711 0.000001 A6122_2539 hypothetical protein CcpA Enterococcus faecium reduced virulence PHI:4564 1.00E-16 A6122_2544 hypothetical protein VTL1 Magnaporthe oryzae unaffected pathogenicity PHI:2032 8.00E-20 A6122_2548 iron dicitrate ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 2.00E-29 A6122_2550 hypothetical protein CcpA Enterococcus faecium reduced virulence PHI:4564 5.00E-24 A6122_2554 two-component system response regulator covR Streptococcus pyogenes reduced virulence PHI:4846 3.00E-40 A6122_2555 histidine kinase SaeS Staphylococcus aureus mixed PHI:4683 5.00E-22 A6122_2557 hypothetical protein ALO1 Candida albicans reduced virulence PHI:197 1.00E-21 A6122_2567 short-chain dehydrogenase fabG1 Ralstonia solanacearum lethal PHI:5271 2.00E-39 A6122_2569 4-aminobutyrate aminotransferase pvdH Pseudomonas aeruginosa unaffected pathogenicity PHI:5059 1.00E-25 A6122_2571 gamma-glutamyltranspeptidase GGT Helicobacter pylori unaffected pathogenicity PHI:3146 6.00E-24 A6122_2573 ornithine--oxo-acid transaminase argD Erwinia amylovora mixed PHI:3126 1.00E-60 A6122_2575 hypothetical protein PlCBP49 Paenibacillus larvae reduced virulence PHI:3139 2.00E-10 A6122_2577 hypothetical protein Rv2702 Mycobacterium tuberculosis unaffected pathogenicity PHI:3636 0.000008 A6122_2580 glycosyl transferase CHIP6 Colletotrichum gloeosporioides reduced virulence PHI:243 0.0000001 A6122_2586 zinc-binding dehydrogenase PKS1 Cochliobolus heterostrophus reduced virulence PHI:55 8.00E-10 A6122_2588 MarR family transcriptional regulator ohrR Vibrio cholerae reduced virulence PHI:5549 1.00E-39 A6122_2590 hypothetical protein GzHMG035 Fusarium graminearum unaffected pathogenicity PHI:1503 1.00E-12 A6122_2597 hypothetical protein EutR Salmonella enterica reduced virulence PHI:5299 3.00E-11 A6122_2598 phosphoribosylformylglycinamidine cyclo-ligase ADE5 Fusarium graminearum reduced virulence PHI:744 9.00E-71 A6122_2600 amidophosphoribosyltransferase ADE4 Saccharomyces cerevisiae reduced virulence PHI:502 9.00E-151 A6122_2602 transcriptional regulator OxyR Escherichia coli reduced virulence PHI:3714 2.00E-12 A6122_2603 hypothetical protein S5nA Lactococcus lactis increased virulence (hypervirulence) PHI:5386 4.00E-37 A6122_2605 phosphoribosylamine--glycine ligase ADE5 Fusarium graminearum reduced virulence PHI:744 1.00E-81 A6122_2608 NAD(P)-dependent oxidoreductase fabG1 Ralstonia solanacearum lethal PHI:5271 1.00E-39 A6122_2618 glutathione peroxidase MoHYR1 Magnaporthe oryzae reduced virulence PHI:2356 2.00E-34 A6122_2621 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 3.00E-42 A6122_2632 NAD(P)-dependent oxidoreductase fabG1 Ralstonia solanacearum lethal PHI:5271 3.00E-39 A6122_2634 hypothetical protein EutR Salmonella enterica reduced virulence PHI:5299 3.00E-16 A6122_2638 MFS transporter VdSge1 Verticillium dahliae loss of pathogenicity PHI:2731 0.00000003 A6122_2641 catalase CAT1 Candida albicans reduced virulence PHI:106 2.00E-130 A6122_2642 Fur family transcriptional regulator PerR Staphylococcus aureus reduced virulence PHI:4991 1.00E-14 A6122_2643 septum formation initiator CD3284 Clostridium difficile increased virulence (hypervirulence) PHI:4637 1.00E-30 A6122_2648 kojibiose phosphorylase atc1 Candida parapsilosis reduced virulence PHI:5349 4.00E-15 A6122_2649 haloacid dehalogenase rhr2 Candida albicans reduced virulence PHI:4992 0.0000001 A6122_2653 hypothetical protein CD3284 Clostridium difficile increased virulence (hypervirulence) PHI:4637 5.00E-13 A6122_2656 oxidoreductase CSH1 Candida albicans reduced virulence PHI:419 3.00E-34 A6122_2657 aldo/keto reductase CSH1 Candida albicans reduced virulence PHI:419 3.00E-15 A6122_2658 LysR family transcriptional regulator OxyR Escherichia coli reduced virulence PHI:3714 2.00E-25 A6122_2659 hypothetical protein Vatr1 Clavibacter michiganensis reduced virulence PHI:3027 0.00000006 A6122_2660 transporter MmpL4a Mycobacterium abscessus increased virulence (hypervirulence) PHI:5400 2.00E-12 A6122_2661 hypothetical protein bpaC Burkholderia pseudomallei reduced virulence PHI:4668 0.00000002 A6122_2662 chorismate mutase pchB Pseudomonas aeruginosa unaffected pathogenicity PHI:5058 0.0000007 A6122_2664 Zn-dependent alcohol dehydrogenase Abd1 Parastagonospora nodorum unaffected pathogenicity PHI:2255 1.00E-20 A6122_2675 NADPH:quinone reductase PKS4_(ZEA1) Fusarium graminearum unaffected pathogenicity PHI:714 1.00E-14 A6122_2678 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 3.00E-42 A6122_2686 hypothetical protein hopA1 Pseudomonas cichorii mixed PHI:3165 1.00E-33 A6122_2688 transposase HMPREF0351_10118 (WxL_locusC) Enterococcus faecium reduced virulence PHI:5205 5.00E-59 A6122_2714 methionine ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 2.00E-31 A6122_2716 cold-shock protein VAD1 Cryptococcus neoformans reduced virulence PHI:423 1.00E-74 A6122_2717 L-idonate 5-dehydrogenase Xdh1 Parastagonospora nodorum unaffected pathogenicity PHI:2256 9.00E-39 A6122_2718 gluconate 5-dehydrogenase fabG1 Ralstonia solanacearum lethal PHI:5271 1.00E-40 A6122_2720 ArsR family transcriptional regulator kdgR Dickeya solani unaffected pathogenicity PHI:4175 2.00E-12 A6122_2722 3-hydroxyisobutyrate dehydrogenase hpd1 Candida albicans reduced virulence PHI:4046 0.000000002 A6122_2724 gluconolactonase LHC1 Cryptococcus neoformans reduced virulence PHI:4197 3.00E-20 A6122_2728 molybdate-binding protein modA10 Haemophilus influenzae increased virulence (hypervirulence) PHI:5422 5.00E-19 A6122_2731 molybdenum ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 1.00E-26 A6122_2732 hypothetical protein hrpx Pantoea stewartii reduced virulence PHI:2473 0.000009 A6122_2733 hypothetical protein dosR_(devR) Mycobacterium tuberculosis increased virulence (hypervirulence) PHI:3614 1.00E-19 A6122_2738 oxidoreductase NIA1 Parastagonospora nodorum unaffected pathogenicity PHI:2336 8.00E-11 A6122_2742 hypothetical protein covR Streptococcus pyogenes reduced virulence PHI:4846 2.00E-41 A6122_2743 hypothetical protein CovRS Streptococcus pyogenes increased virulence (hypervirulence) PHI:4097 1.00E-25 A6122_2746 NAD-dependent dehydratase USX1 Cryptococcus neoformans loss of pathogenicity PHI:274 0.000002 A6122_2749 sodium:proton antiporter nhaA Escherichia coli reduced virulence PHI:3266 2.00E-61 A6122_2751 pyridine nucleotide-disulfide oxidoreductase Rv0392c Mycobacterium tuberculosis unaffected pathogenicity PHI:3629 0.0000004 A6122_2762 aldo/keto reductase CSH1 Candida albicans reduced virulence PHI:419 1.00E-38 A6122_2763 non-ribosomal peptide synthetase Cif_B Pseudomonas cichorii reduced virulence PHI:4836 2.00E-21 A6122_2764 hypothetical protein cipA Pseudomonas cichorii reduced virulence PHI:4837 8.00E-38 A6122_2766 hypothetical protein HsvC Erwinia amylovora mixed PHI:2466 1.00E-21 A6122_2769 NikQ protein xrp14 Xanthomonas oryzae reduced virulence PHI:4138 2.00E-30 A6122_2771 carboxylase HsvB Erwinia amylovora mixed PHI:2465 4.00E-14 A6122_2773 ABC transporter ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 8.00E-31 A6122_2778 transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 1.00E-30 A6122_2779 sugar ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 3.00E-12 A6122_2780 thiamine pyrophosphate-binding protein MoIlv2 Magnaporthe oryzae loss of pathogenicity PHI:3975 6.00E-28 A6122_2783 sugar ABC transporter ATP-binding protein ABC4 Magnaporthe oryzae loss of pathogenicity PHI:2067 7.00E-12 A6122_2789 MFS transporter MoST1 Magnaporthe oryzae unaffected pathogenicity PHI:4024 8.00E-43 A6122_2812 histidinol-phosphatase LmABCB3 Leishmania major reduced virulence PHI:5554 1.00E-16 A6122_2816 cation transporter FGSG_09759 Fusarium graminearum unaffected pathogenicity PHI:1088 5.00E-12 A6122_2819 hypothetical protein chp-7 Clavibacter michiganensis effector (plant avirulence determinant) PHI:4931 4.00E-33 A6122_2820 LacI family transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 2.00E-27 A6122_2824 hypothetical protein EutR Salmonella enterica reduced virulence PHI:5299 2.00E-13 A6122_2827 beta-glucosidase Avenacinase_gene Gaeumannomyces graminis mixed PHI:24 4.00E-36 A6122_2831 LacI family transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 6.00E-31 A6122_2835 hypothetical protein AS87_04050 Riemerella anatipestifer reduced virulence PHI:4819 7.00E-11 A6122_2836 hypothetical protein aiiA Burkholderia glumae mixed PHI:2340 8.00E-10 A6122_2840 gamma-glutamyltransferase GGT Helicobacter pylori unaffected pathogenicity PHI:3146 4.00E-95 A6122_2847 hypothetical protein ypo2062 Yersinia pestis reduced virulence PHI:4185 7.00E-22 A6122_2849 oxidoreductase ABA4 Magnaporthe oryzae reduced virulence PHI:5440 1.00E-41 A6122_2850 AMP-dependent synthetase F-avi3342 Agrobacterium vitis loss of pathogenicity PHI:2894 2.00E-25 A6122_2856 short-chain dehydrogenase MGG_00056 Magnaporthe oryzae reduced virulence PHI:784 8.00E-12 A6122_2862 ATPase ypmt1,66c Yersinia pestis reduced virulence PHI:4183 0.000003 A6122_2870 MarR family transcriptional regulator slyA Salmonella enterica reduced virulence PHI:2678 0.00000003 A6122_2881 pyridine nucleotide-disulfide oxidoreductase Glr1 Candida albicans reduced virulence PHI:4904 9.00E-41 A6122_2886 LacI family transcriptional regulator CcpA Enterococcus faecium reduced virulence PHI:4564 7.00E-38 A6122_2896 ABC transporter ATP-binding protein LmABCB3 Leishmania major reduced virulence PHI:5554 3.00E-17 A6122_2900 ABC transporter ATP-binding protein pstB Xanthomonas citri loss of pathogenicity PHI:3412 1.00E-17 A6122_2901 PadR family transcriptional regulator AphA Vibrio cholerae reduced virulence PHI:711 0.000009 A6122_2914 single-stranded DNA-binding protein SSBX Xanthomonas oryzae reduced virulence PHI:2737 0.00000002 A6122_2920 thioredoxin thioredoxin_1 Salmonella enterica reduced virulence PHI:2644 1.00E-34 A6122_2921 GntR family transcriptional regulator Vatr2 Clavibacter michiganensis reduced virulence PHI:3028 0 A6122_2924 chromosome partitioning protein Rv3213c Mycobacterium tuberculosis increased virulence (hypervirulence) PHI:3628 3.00E-52 aAccession Id in the PHI-base database bE -value calculated by the BLASTP algorithm 1052 12

1795

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