The National Cancer Institute at Frederick

What is it? Where is it?

Story on page 16.

Dr. John Niederhuber Nominated as SEPTEMBER 2006 13th Director of NCI IN THIS ISSUE Dr. Elias Zerhouni, director of the National Institutes of Health, Science Today 3 announced last August that President Bush intends to name Dr. John Our Partners 4 Niederhuber the 13th director Off-Site Programs 6 of the National Cancer Institute. Dr. Niederhuber has been Acting Platinum Publications 8 Director since June and was Technology Transfer Branch 11 also NCI’s Deputy Director for Translational and Clinical Sciences. Environment, Health, and Safety In his e-mail message to NCI Program 12 employees, Dr. Zerhouni said, “A Where Are They Now? 15 renowned surgeon and research scientist, his career before coming The Poster Puzzler 16 to the NIH included positions at the University of Wisconsin School Poster People Profi le 17 of Medicine, Stanford University, FME Machine Shop 18 Johns Hopkins University School of Medicine, and the University Campus Improvement of Michigan. While at the Committee 19 University of Wisconsin, he directed Immediately before joining the NIH, Outreach and Special the University’s Comprehensive Dr. Niederhuber chaired the National Programs 20 Cancer Center supported by NCI. Cancer Advisory Board.” Ø Reporting In 21 2004 Chemistry Nobel Laureate Lectures Here Farmers’ Market 22 Frederick Employee Diversity Thirty years ago, not many people Recently, Dr. Hershko, a member of Team 24 realized that cancer can be caused by the the National Academy of Sciences, New Faces at NCI-Frederick 25 lack of degradation of an oncoprotein lectured here to an SRO crowd and or, on the other hand, by too rapid a simultaneous videocast to NCI- Special Events 26 degradation of a tumor suppressor Bethesda. Dr. Hershko noted in his Write When You Get Work 27 protein. Alterations in the ubiquitin talk that his interest in the ubiquitin system play a role in various cancers, system began in the 1970s as he Data Management Services 28 such as colon, breast, uterine cervical sought to understand why energy SAIC-Frederick, Inc. 29 carcinoma, sarcomas, aggressive is required for degradation. He cancers, and chromosomal instabilities. developed a biochemical analysis, Wilson Information Services Now, thanks to Dr. Avram Hershko, and his fi rst results, published in the Corporation 30 Nobel Laureate in Chemistry in 2004, Journal of Biological Chemistry, and his colleagues, study of the ubiquitin revealed that degradation of TAT Poster Puzzler Winner 31 system has become an important tool in (tyrosine aminotransferase) was Employment Opportunities 32 the battle against cancer. continued on page 2 2004 Chemistry Nobel Laureate Lectures Here continued from page 1 arrested by inhibitors of cellular green light of his pointer darted over infl ammation, immune response, and energy production. the screen like a glowing fi refl y as regulation of gene expression. The Nobel Prize Committee’s press he pointed out important aspects of That fi rst process—control of cell release for the 2004 Nobel Prize fi gures that summarized what has been division—interests Dr. Hershko the noted that in protein degradation, the learned and that described the new most. He began researching cell division ubiquitin molecule attaches itself to work he’s doing in this fi eld. in the 1990s, noting (again, with his gentle humor) that while most people use clams to make chowder, he fi nds them more useful in his studies of cell division because “It’s easy to make extracts from the clam.” Tongue in cheek, Dr. Hershko concluded that he’s learned two valuable lessons in his years of research: (1) Choose as your research subject something important The fi gure above, taken from Dr. Hershko’s but not yet interesting to Nobel Prize lecture in 2004, shows the others; (2) biochemistry is control of the regulation of cell division by still much needed! In all of his the Anaphase-Promoting Complex Cyclosome research, he pointed out, he ubiquitin ligase.[Source: Figure courtesy of has remained true to his calling Dr. Avram Hershko.] as a biochemist. Dr. Hershko’s pioneering work on ubiquitin, an important molecular target for Dr. Avram Hershko, winner of the many diseases, has brought Nobel Prize in Chemistry, 2004 him and his colleagues numerous awards. In 2000 he a protein to be destroyed and stays He added in a humorous aside and colleagues received the prestigious with it, alerting the proteasome that that he realized “Not many people Albert Lasker Award for Basic Medical a protein is headed for processing. were interested in the fi eld” when Research and in 2001 the Louisa Gross “Shortly before the protein is squeezed he started. But times have changed. Horwitz Prize, given to recognize into the proteasome, its ubiquitin Today, protein degradation (protein “exceptional accomplishments label is disconnected for re-use,” breakdown and disposal) is considered in biological and biochemical the press release explained. “When one of the most important of the cell’s research.” And, of course, the ultimate the degradation does not work cyclical functions and is involved recognition of his work came in 2004 correctly,” we become sick, the press in processes such as control of when he, Aaron Ciechanover, and release continued. Dr. Hershko’s cell division, signal transduction, Irwin Rose were jointly awarded the and his colleagues’ work will be embryonic development, apoptosis, Nobel Prize in Chemistry. Ø useful in developing drugs to battle Circadian clocks, response to many diseases. Dr. Hershko delineated the stages in Web sites of interest biochemical analysis of this complex system—the steps in making a cell- • http://nobelprize.org/nobel_prizes/chemistry/laureates/2004/hershko- free extract that reproduces the cell- lecture.html cycle process in the test tube—such as • http://nobelprize.org/nobel_prizes/chemistry/laureates/2004/hershko- the breakdown of protein/degradation; slides.pdf fractionation, etc., all the way to reconstituting the cell system. The • http://www.technion.ac.il/ Ø

The NCI-Frederick Poster 2 September 2006 Science Today

ABCC Provides Resource The revolution in biology produces the well-known, fully sequenced for Scientifi c Computing data ever-increasing in both mammalian genomes such as the complexity and volume. To help head human, mouse, dog, chimp and rat The Advanced Biomedical off potential problems resulting from and several yeast and other microbial Computing Center (ABCC) is an this growth, ABCC has large capacity genomes, some species such as the cat NCI-funded center dedicated to storage to house the data and high and elephant have been undergoing providing cyber infrastructure for bandwidth to rapidly access the data. survey sequencing. Others are being data-intensive computing to the ABCC has further helped confront constantly added to this list. Early NCI intra- and extramural scientifi c this problem by providing access to, during the initial releases of these communities. Since 1985, the Center and help with designing relational genomes, it became apparent that has been providing NCI-Frederick databases and Web access tools to there was a need for more rapid tools with networking, computer security, those databases. ABCC has both for genome annotation. This was computational infrastructure, scientifi c mySQL and Oracle databases online, further evidenced by the release of applications and databases, as well as hosted on multiple servers with a high multiple versions of each genome as scientifi c collaborations, consultation data capacity. more information and more accurate and assistance. The Center has added One example of this rapid expansion sequence assemblies were produced. or expanded many of these capabilities is in the area of high-throughput ABCC was able to speed up a tool in recent years in response to the genome sequencing. Now that whole that identifi ed short tandem repeat growing needs resulting from changes genome sequences for various species sequences within chromosomes by in high-throughput biology and new are becoming available, the demand more than 100-fold. Many of these technologies. Services and resources at for functional and comparative tools are available through Web ABCC are free. genomic analysis and annotation interfaces and the results are stored in ABCC administers a heterogeneous is escalating. These comparative databases for instant retrieval. computational infrastructure to studies are revealing insights in tailor hardware to specifi c software the investigation of human disease In upcoming issues of the Poster, we applications and databases. ABCC genes. Since these gigabytes of will profi le some of these tools. Ø provides many different scientifi c data can be too large for a desktop applications from both commercial computer to handle, and public sources as well as the ABCC provides the large databases required for their operation. storage and computational For example, ABCC maintains local resources, as well as the and up-to-date copies of many of bioinformatics expertise the sequences from NCBI for use necessary for embarking with the local installations of BLAT on these kinds of and BLAST genome searching explorations. programs. Besides providing existing bioinformatics tools and databases, Tools for Data Mining ABCC works with scientists to At ABCC, bioinformatics develop novel applications and modify researchers and analysts existing ones. continuously work on developing tools that aid Wide Array of Resources laboratory researchers In addition to providing the hardware as well as mine the vast and networking for these applications, amounts of experimental ABCC provides both classroom and data. One of the major one-on-one instruction in using these responsibilities of the applications. The classes include bioinformatics team is many topics, from basic Unix usage assisting in the sequence to sequence analysis and molecular analysis and annotation modeling, docking, and other of survey sequenced structural analysis methods. genomes. Apart from

The NCI-Frederick Poster 3 September 2006 Our Partners

The Colonel Loves Her Job!

The National Cancer Institute vaccines, and creating other at Frederick partners with many products that safeguard the health intramural and extramural entities as of our soldiers. Currently, the post we search for cures for cancer, AIDS, is home to a number of Army and other infectious diseases. Our own medical units, among them the U.S. campus is adjacent to the stronghold Army Medical Materiel Activity, of one of those partners: Fort Detrick. an arm of the U.S. Army Medical Often, our two entities intertwine—in Research and Materiel Command shared commitment to our nation’s (USAMRMC). “Everything we health, in shared research projects, in develop here goes to all four shared facilities, and in strong leaders services, once they’re approved by who love what they do. the Defense Medical Standardization Just as we have Dr. Craig Reynolds Board. Our research puts products in Partnering with NCI-Frederick heading NCI-Frederick, the army has the fi eld that really help the soldiers,” Colonel Mary Colonel Deutsch explained. Colonel Deutsch noted that Fort Deutsch, Detrick partners with NCI-Frederick Deputy A Colonel’s Job Is “in a lot of our initiatives,” such Installation Never Done as collaborating on some research Commander of Colonel Deutsch’s duty “day” is projects, allowing NCI-Frederick Fort Detrick, almost 24 hours. “The responsibility employees to use most of the free who provides is not just the mission of taking care programs and services the base offers; vitally of my soldiers. It’s making sure of and participating in annual events, important the security of the installation, family such as the May Spring Research support housing, the buildings, infrastructure. Festival and the July Take Your Child services to Anything could happen at any time. to Work Day. research, Having that situational awareness logistics, at all times is probably the most and communications at Fort Detrick. demanding part of the job. As an army And, as she enthusiastically noted in installation, we have responsibilities a recent interview for this newsletter, for the perimeter and security of the she loves her job and the people she installation. I take that as one of my works with. most important jobs,” she said. Previously in charge of the Medical Medical Groups Long a Logistics Battalion, a fi eld organization Part of Fort Detrick that provides medical products to Medical groups have formed an America’s armed forces, Colonel integral part of Fort Detrick since Deutsch came to Fort Detrick in 2000, 1942. In NCI-Frederick’s mission serving as chief of staff for Major to make cancer easily treatable, thus General Lester Martinez-Lopez, the helping to safeguard the nation’s previous installation and USAMRMC health, we form partnerships with commander. Being stationed here both intramural and extramural during Fort Detrick’s recent growth investigators, such as with the U.S. has “paid tremendous dividends for my An important part of this partnership Army Medical Research Institute of learning curve. It is fun, challenging, is the civilian support in many Infectious Diseases (USAMRIID). exciting. One of the best positions I matters connected to infrastructure. As part of that national concern, have ever had,” she said. For example, Colonel Deutsch said, Fort Detrick researchers also focus on she considers us strong partners in fi nding cures for cancer, developing developing effi ciencies in our energy consumption. “We are certainly

The NCI-Frederick Poster 4 September 2006 Our Partners partners in that. Every mission dollar relationship. And now we are If you want to know more…: that we can save in energy goes toward growing and using some of the things being able to complete our mission,” we’ve worked out with NCI as our • For more on County Colonel Deutsch said. framework for our new partners that facts, go to http://www. Looking at the long-range plans we’re bringing on the installation.” discoverfrederickmd. for Fort Detrick with the Homeland While Fort Detrick is the largest com/fastfacts/documents/ Defense project that is being built command she’s had, Colonel Deutsch FrederickBEF06.pdf. and the changes that brings to the has had no problems commanding as a infrastructure, security, and other woman. She points out that the Army • For more about Fort Detrick, go aspects of the installation, Colonel expects any of its leaders, male or to http://www.detrick.army.mil Deutsch noted that “We get support female, “to be able to stand toe-to-toe from all our ‘mission partners’ because with all of your counterparts. There • “Materiel,” not “material,” we are all working toward the same is just a higher expectation if you are refers to “equipment, apparatus, end—to have a secure workplace going to strive to become a leader, and supplies used by an where we can complete our missions regardless of sex. That’s just expected organization or institution,” in the most effi cient manner. Certainly, of anyone. If you’re not willing to make according to the Merriam- any decisions we make are going to sacrifi ces to do that, then you shouldn’t Webster on-line dictionary, impact NCI, as well as other federal try to be the leader. Fort Detrick is one http://www.m-w.com/cgi-bin/ agencies. We work very hard at of the greatest army installations. It is dictionary?book=Dictionary&v keeping communications open and small, it is defi ned by what we do. It a=materiel. Ø working to come to decisions that we has a purpose and mission in life, to all agree on,” she said. protect not only the war fi ghter, but our She added, “I appreciate the nation. I’m just tremendously honored partnership that we have with NCI. and humbled to be in this position. And It’s a very good, long-standing I love my job!” Ø

Improve Your Speaking Skills Check It Out! Were you looking for information about… NCI-Frederick employees are able to participate in many Fort Detrick Diversity-related information? activities. If you would like to gain • http://diversity.ncifcrf.gov/fl ash_content/default.asp confi dence in oral presentations, you • http://www3.kumc.edu/diversity/ might consider joining a toastmasters • http://life.familyeducation.com/diversity/respecting-equality/34476.html club. Two meetings will be held in October to determine interest. You Hispanic American Month (September 15–October 15)? are welcome to bring your lunch • http://www.clnet.ucla.edu/heritage/hhhispan.htm (or breakfast, in the case of the • http://www.cr.nps.gov/nR/feature/hispanic/ second meeting). • http://www.smithsonianeducation.org/heritage_month/index.html Wed., Oct.11, 11:30 a.m.-1:00 p.m., NCI-Frederick? NCI-Frederick Café Meeting Room • http://saic.ncifcrf.gov/fi tnesschallenge/ Thur., Oct 12, 7:00 a.m.- 8:00 a.m., • http://web.ncifcrf.gov/campus/committees/ Fortt Detrick’s new dining facility • http://web.ncifcrf.gov/research-technologies/default.asp Conference Room on Freedman Drive

Flu updates? If you are interested, contact Major • http://www.pandemicfl u.gov/ Ø Katherine V. Suarez, USAMRIID, at [email protected] or 301-619-2965. Ø

The NCI-Frederick Poster 5 September 2006 Off-Site Programs

Core Genotyping Facility Offers State-of-the-Art Gene Analysis

Editor’s note: From time to time, we offer you a profi le of one of the many NCI- Frederick programs that are off-site so that you can learn a little about your colleagues that you don’t often see at our main campus. If your program has not been profi led, please let the editors know at [email protected]. If your research focus includes genetics, then you may need the resources offered through the Core Genotyping Facility staff, Advanced Technology Center, Gaithersburg. Several Core Genotyping Facility (CGF), people were unable to be present for the photo. comprising both research and development and bioinformatics. for analysis from SNP500Cancer Located in the Advanced Technology CGF uses seven major technology site assays or commercially available Center in Gaithersburg, CGF, platforms to assess human genetic panels. New assays are designed and as part of the NCI’s Division of variation. These include: optimized for ongoing studies. Cancer Epidemiology and Genetics • TaqMan® fl uorescent 5´ nuclease In the Research and Development (DCEG), assesses human genetic cleavage, section, CGF staffers evaluate and variation, including single nucleotide • Fluorescent DNA fragment analysis develop technologies for cancer genomic and sequencing detected by research, focusing on integrated automated capillary electrophoresis “wet” and “dry” analysis. During systems, 2005 and 2006, the CGF completed • MGB Eclipse™ 3´ hybridization a number of signifi cant projects in triggered fl uorescence, DNA quantifi cation, whole genome • SNPlex™ oligonucleotide ligation amplifi cation, whole genome SNP assay (OLA) in combination with analysis, and analytical tool evaluation. PCR chemistry and fl uorescent The Bioinformatics team has DNA fragment analysis, developed a series of tools and • Affymetrix GeneChip® Mapping workfl ows to address internal Arrays for high-throughput workfl ow and to provide public genotype analysis—both custom and 500 k, • Illumina GoldenGate® technology for custom SNP genotyping, and • Illumina Infi nium™ technology with the Sentrix® HumanHap550 Tabassum Bandey and Shaila Sharmeen, BeadChip, powered by the both research associates, prepare to Infi nium™ II assay. hybridize fl uorescently labeled PCR To help meet NCI the challenge products to fi ber-optic arrays used for of curing cancer by 2015, CGF Illumina’s GoldenGate® Assay. supports the genotyping and DNA sequencing needs of the DCEG and polymorphisms (SNPs) and other NCI’s Center for Cancer Research. types of genetic variation, such as The facility performs high-throughput microsatellites or insertion/deletion genotyping and sequencing to support mutations, in many of NCI’s genetic analysis for a broad range of population and family studies. projects for NCI’s intramural research Colin Stefan, research technician, program. CGF senior staff works with precipitates DNA for the PCR step investigators to select the best SNPs in Illumina’s GoldenGate® Assay.

The NCI-Frederick Poster 6 September 2006 Off-Site Programs access and tools for analysis of handling, candidate gene assay genetic variation. These include development, and genotyping services Genewindow, the SNP500Cancer on a fee-for-service basis, it cannot data base, and Tagzilla (a program perform “cost comparisons” for for selecting tagSNPs based on the genotyping services. “greedy” algorithm). For example, Another project in which CGF on the SNP500Cancer Web site is involved is the Cancer Genetic (http://snp500cancer.nci.nih.gov), you Markers of Susceptibility (CGEMS) can search for SNPs by any of several initiative (Cancer Bulletin, 2[16]:7, 2005; http://cgems.cancer. gov) to perform dense whole genome scans that identify and validate susceptibility genes in the induction and progression of prostate and breast Dr. Stephen Chanock, director of the cancer. CGF is conducting Core Genotyping Facility, reviews notes most of the laboratory work, for a project. including bioinformatic analysis, for this project. Dr. Stephen Chanock, NCI, heads the CGEMS represents a Core Genotyping Facility. Senior staff three-year NCI initiative includes Dr. Meredith Yeager-Jeffery, Herbert Higson, research technician, creates coordinated among the Scientifi c Director; Robert Welch, a PCR master mix for a TaqMan® SNP DCEG, CGF, and the NCI Deputy Director; Dr. Laurie Burdett, genotyping assay. Offi ce of Cancer Genomics. Sequencing head; and Bernice Packer, CGEMS is a resource for Bioinformatics head. key links: SNP identifi er, gene symbol, the strategic partnerships between For information about CGF and its gene alias, the chromosome location, intramural and extramural groups work, contact Dr. Chanock (chanocks@ or a gene ontology pathway. joining forces to incorporate genomic mail.nih.gov) or Dr. Yeager-Jeffery Please note that while CGF can and other emerging technologies in ([email protected]). Ø provide investigators with sample large-scale epidemiologic studies.

Bow Season Opens

Fort Detrick’s white-tail deer bow military and their dependents season began September 15, 2006, and (12 and older); Fort Detrick continues through January 31, 2007. active and retired civilian To be eligible to hunt on Fort Detrick employees and their dependents property, you must have a Maryland (12 and older); and government Resident or Nonresident Consolidated contractor personnel at Fort Hunting License or regular hunting Detrick and their dependents license with an archery stamp and (12 and older). must comply with the Maryland Qualifi cation testing continues Department of Natural Resources through December 15, Monday– hunting regulations. You must also Friday, 4:00–5:00 p.m., at the pass the Fort Detrick Bow Hunting Nallin Farm Multi-Purpose Qualifi cation Test to receive a Fort Events Complex. Call 301–619– Detrick hunting permit. Those eligible 2759/2957 for an appointment include: active duty and retired or for information. Ø

The NCI-Frederick Poster 7 September 2006 Platinum Highlight

Dr. Lino Tessarollo recombination in mice. He established and, thus, are potential therapeutic Acting Director, Mouse Cancer the Special Program in Germline targets for the management of Genetics Program Mutation in 1994 to generate targeted neurodegenerative disorders such mouse models. He later formed the as Parkinson’s and Alzheimer’s Neural Development Group and diseases. Neurotrophin receptors established a mouse gene targeting are also frequently overexpressed in program for the National Cancer human cancer, including pancreatic Institute. In 1999, he joined the Center and prostate carcinoma, melanoma, for Cancer Research, NCI, where medulloblastoma, and neuroblastoma. he directs the Neural Development “Our work has established a Section and the Gene Targeting non-catalytic TrkC isoform as an Facility in the Mouse Cancer unsuspected upstream activator Genetics Program. He is currently of ARF6, a regulator of processes Acting Director of the Mouse Cancer important for cell motility, raising an Genetics Program. important question concerning the role Dr. Tessarollo’s research focuses on of Trk receptors in tumor progression,” the signals controlling mammalian Dr. Tessarollo said. While expression Dr. Tessarollo earned his Ph.D. nervous system cell proliferation of Trk receptors has been found in in biological sciences from the and survival. “Our goal,” he said, many tumor types, how the favorable University of Padua, Italy, in 1987, “is to identify specifi c pathways or negative prognosis is associated with where he studied the molecular that can be activated to promote the expression of a specifi c Trk receptor mechanisms underlying tumors survival of cell populations affected is not understood. “Our fi ndings,” induced by the Moloney murine in neurodegeneration, thus preventing he explained, “raise the intriguing sarcoma virus in mouse and rat. uncontrolled cell proliferation possibility that expression of truncated In 1990, he came as a postdoctoral leading to cancer.” The neurotrophin receptors alone, or in association fellow to the ABL-Basic Research growth factors and their Trk tyrosine with the tyrosine kinase isoforms, Program at NCI-Frederick, where kinase receptors are critical to the may account for some tumor growth he helped develop new technology development and maintenance of characteristics, including proliferation in gene targeting by homologous the mammalian nervous systems and metastatic potential.” Ø

Esteban PF, Yoon HY, Becker J, Dorsey SG, Caprari P, Palko ME, Coppola V, Saragovi HU, Randazzo PA ,Tessarollo L.

A Kinase-Defi cient TrkC Receptor Isoform Activates Arf6-Rac1 Schematic representation of the Signaling through the Scaffold Protein Tamalin newly identifi ed NT-3-activated J Cell Biol 173(2):291–299, 2006 signaling pathway leading to cell ruffl ing and actin reorganization. Neurotrophins play an essential ligand-dependent manner. Moreover, role in mammalian development. NT3 initiation of this complex leads to Most of their functions have been activation of the Rac1 GTPase through attributed to activation of the kinase- adenosine diphosphate-ribosylation active Trk receptors and the p75 factor 6 (Arf6). At the cellular level, neurotrophin receptor. Truncated Trk NT3 binding to TrkCT1–tamalin receptor isoforms lacking the kinase induces Arf6 translocation to the domain are abundantly expressed membrane, which in turn causes during development and in the adult; membrane ruffl ing and the formation however, their function and signaling of cellular protrusions. Thus, our data capacity is largely unknown. We identify a new signaling pathway show that the neurotrophin-3 (NT3) elicited by the kinase-defi cient TrkCT1 For complete article and details on TrkCT1-truncated receptor binds receptor. Moreover, we establish NT3 the fi gure, go to http://www.jcb.org/ to the scaffold protein tamalin in a as an upstream regulator of Arf6. Ø cgi/content/full/173/2/291. Ø

The NCI-Frederick Poster 8 September 2006 Platinum Publications

The following 36 articles have been selected murine neuroblastoma tumors. J Immunol in the central nervous system. J Biol Chem from a quarterly listing of publications in 12 of 176(10):6302–6312, 2006. 281(26):17681–17688, 2006. the most prestigious science journals. Shorts L, Weiss JM, Lee JK, Welniak McCauslin CS, Heath V, Colangelo AM, Apoptosis LA, Subleski J, Back T, Murphy WJ, Malik R, Lee S, Mallei A, Mocchetti Wiltrout RH. Stimulation through CD40 I, Johnson PF. C/EBPdelta and CREB Chandra D, Bratton SB, Person MD, on mouse and human renal cell carcinomas mediate inducible expression of the nerve Tian YA, Martin AG, Ayres M, Fearn- triggers cytokine production, leukocyte growth factor gene in the central nervous head HO, Gandhi V, Tang DG. Intracel- recruitment, and antitumor responses that system. J Biol Chem 281(26):17681- lular nucleotides act as critical prosurvival can be independent of host CD40 expres- 17688, 2006. factors by binding to cyctochrome c and sion. J Immunol 176(11):6543–6552, 2006. inhibiting apoptosome. Cell 125(7):1333– Genetics 1346, 2006. DNA: Replication, Repair, Cui CY, Hashimoto T, Grivennikov Cellular Immunology and and Recombination SI, Piao Y, Nedospasov SA, Schless- Immune Regulation Bampi C, Bibillo A, Wendeler M, inger D. Ectodysplasin regulates the Divita G, Gorelick RJ, Le Grice SFJ, lymphotoxin-beta pathway for hair Kim BG, Li CL, Qiao WH, Mamura M, Darlix JL. Nucleotide excision repair differentiation. Proc Natl Acad Sci USA Kasperczak B, Anver M, Wolfraim L, and template-independent addition by 103(24):9142–9147, 2006. Hong S, Mushinski E, Potter M, Kim HIV-1 reverse transcriptase in the pres- SJ, Fu XY, Deng CX, Letterio JJ. Smad4 Hematopoiesis ence of nucleocapsid protein. J Biol Chem signalling in T cells is required for sup- Suh HC, Gooya J, Renn K, Friedman 281(17):11736–11743, 2006. pression of gastrointestinal cancer. Nature AD, Johnson PF, Keller JR. C/EBP 441(7096):1015–1019, 2006. Epidemiology and Prevention alpha determines hematopoietic cell Michaud DS, Daugherty SE, Berndt fate in multipotential progenitor cells by Liepinsh DJ, Grivennikov SI, Klar- SI, Platz EA, Yeager M, Crawford ED, inhibiting erythroid differentiation and mann KD, Lagarkova MA, Drutskaya Hsing A, Huang WY, Hayes RB. Genetic inducing myeloid differentiation. Blood MS, Lockett SJ, Tessarollo L, McAuliffe polymorphisms of interleukin-1B (IL-1B), 107(11):4308–4316, 2006. M, Keller JR, Kuprash DV, Nedospasov IL-6, IL-8, and IL-10 and risk of prostate Hemostasis, Thrombosis, SA. Novel lymphotoxin alpha (LT alpha) cancer. Cancer Res 66(8):4525–4530, 2006. knockout mice with unperturbed tumor and Vascular Biology necrosis factor expression: Reassessing LT Endocrinology Calvani M, Rapisarda A, Uranchimeg alpha biological functions. Mol Cell Biol Nunez NP, Oh WJ, Rozenberg J, Perella B, Shoemaker RH, Melillo G. Hypoxic 26(11):4214–4225, 2006. C, Anver M, Barrett JC, Perkins SN, induction of an HIF-1 alpha-dependent Berrigan D, Moitra J, Varticovski L, bFGF autocrine loop drives angiogen- Mason LH, Willette-Brown J, Taylor Hursting SD, Vinson C. Accelerated esis in human endothelial cells. Blood LS, McVicar DW. Regulation of Ly49D/ tumor formation in a fatless mouse with 107(7):2705–2712, 2006. DAP12 signal transduction by Src-family type 2 diabetes and infl ammation. Cancer HIV kinases and CD45(1,2). J Immunol Res 66(10):5469–5476, 2006. Al-Mawsawi LQ, Fikkert V, Dayam R, 176(11):6615–6623, 2006. Enzyme Catalysis and Witvrouw M, Burke TR, Jr., Borch- Chemokines, Cytokines, Regulation ers CH, Neamati N. Discovery of a and Interleukins Dash C, Fisher TS, Prasad VR, Le Grice small-molecule HIV-1 integrase inhibi- Zhang N, Yang D, Dong HF, Chen Q, SF. Examining interactions of HIV-1 tor-binding site. Proc Natl Acad Sci USA Dimitrova DI, Rogers TJ, Sitkovsky reverse transcriptase with single-stranded 103(26):10080-10085, 2006. M, Oppenheim JJ. Adenosine A2a template nucleotides by nucleoside analog receptors induce heterologous desensi- interference. J Biol Chem 2006 [Epub Picker LJ, Reed-Inderbitzin EF, Hagen tization of chemokine receptors. Blood ahead of print]. SI, Edgar JB, Hansen SG, Legasse 108(1):38–44, 2006. A, Planer S, Piatak M, Jr., Lifson JD, Genes: Structure and Maino VC, Axthelm MK, Villinger Clinical Immunology Regulation F. IL-15 induces CD4 effector memory Khan T, Stauffer JK, Williams R, McCauslin CS, Heath V, Colangelo AM, T-cell production and tissue emigration Hixon JA, Salcedo R, Lincoln E, Back Malik R, Lee S, Mallei A, Mocchetti I, in nonhuman primates. J Clin Invest TC, Powell D, Lockett S, Arnold AC, Johnson PF. CAAT/enhancer-binding 116(6):1514–1524, 2006. Sayers TJ, Wigginton JM. Protea- protein delta and cAMP-response ele- some inhibition to maximize the apop- ment-binding protein mediate inducible totic potential of cytokine therapy for expression of the nerve growth factor gene

The NCI-Frederick Poster 9 September 2006 Platinum Publications

Host Defense prevention of maternal-to-fetal HIV-1 Pharmacology Abdool K, Cretney E, Brooks AD, Kelly transmission. Proc Natl Acad Sci USA Al-Mawsawi LQ, Fikkert V, Dayam JM, Swann J, Shanker A, Bere EW, Jr., 103(18):7094–7099, 2006. R, Witvrouw M, Burke TR, Borch- Yokoyama WM, Ortaldo JR, Smyth ers CH, Neamati N. Discovery of a MJ, Sayers TJ. NK cells use NKG2D to Si ZH, Vandegraaff N, O’HUigin C, small-molecule HIV-1 integrase inhibi- recognize a mouse renal cancer (Renca), Song B, Yuan W, Xu C, Perron M, Li tor-binding site. Proc Natl Acad Sci USA yet require intercellular adhesion mol- X, Marasco WA, Engelman A, Dean M, 103(26):10080–10085, 2006. Sodroski J. Evolution of a cytoplasmic ecule-1 expression on the tumor cells for Protein Structure and Folding optimal perforin-dependent effector func- tripartite motif (TRIM) protein in cows tion. J Immunol 177(4):2575–2583, 2006. that restricts retroviral infection. Proc Natl Gattis JL, Washington AV, Chisholm Acad Sci USA 103(19):7454–7459, 2006. MM, Quigley L, Szyk A, McVicar Infl ammation Molecular Basis of Cell and DW, Lubkowski J. The structure of the Coppola V, Barrick CA, Bobisse S, extracellular domain of triggering re- Rodriguez-Galan MC, Pivetta M, Developmental Biology ceptor expressed on myeloid cells like Reynolds D, Howard OM, Palko ME, Waheed AA, Ablan SD, Mankowski transcript-1 and evidence for a naturally Esteban PF, Young HA, Rosato A, Tes- MK, Cummins JE, Ptak RG, Schaffner occurring soluble fragment. J Biol Chem sarollo L. The scaffold protein cybr is CP, Freed EO. Inhibition of HIV-1 rep- 281(19):13396–13403, 2006. required for cytokine-modulated traffi ck- lication by amphotericin B methyl ester: ing of leukocytes in vivo. Mol Cell Biol selection for resistant variants. J Biol Kovaleski BJ, Kennedy R, Hong MK, 26(14):5249–5258, 2006. Chem 2006 [Epub ahead of print]. Datta SA, Kleiman L, Rein A, Musier- Mechanisms of Signal Neoplasia Forsyth K. In vitro characterization of the interaction between HIV-1 Gag and human Catalano A, Caprari P, Moretti S, Transduction lysyl-tRNA synthetase. J Biol Chem Faronato M, Tamagnone L, Procopio A. Gamero AM, Potla R, Wegrzyn J, 281(28):19449–19456, 2006. Szelag M, Edling AE, Shimoda K, Link Semaphorin-3A is expressed by tumor cells and alters T-cell signal transduction and DC, Dulak J, Baker DP, Tanabe Y, Prabakaran P, Gan JH, Feng Y, Zhu function. Blood 107(8):3321–3329, 2006. Grayson JM, Larner AC. Activation of ZY, Choudhry V, Xiao XD, Ji XH, Dimi- Tyk2 and Stat3 is required for the apop- Oncogene trov DS. Structure of severe acute respira- totic actions of interferon-beta in primary He YY, Pi J, Huang JL, Diwan BA, tory syndrome coronavirus receptor-bind- pro-B cells. J Biol Chem 281(24):16238– Waalkes MP, Chignell CF. Chronic UVA ing domain complexed with neutralizing 16244, 2006. irradiation of human HaCaT keratinocytes antibody. J Biol Chem 281(23):15829– Medicine induces malignant transformation associ- 15836, 2006. Boillee S, Yamanaka K, Lobsiger CS, ated with acquired apoptotic resistance. Receptors Oncogene 25(26):3680–3688, 2006. Copeland NG, Jenkins NA, Kassiotis Esteban PF, Yoon HY, Becker J, Dorsey G, Kollias G, Cleveland DW. Onset and SG, Caprari P, Palko ME, Coppola Ivanov SV, Salnikow K, Ivanova AV, progression in inherited ALS determined V, Saragovi HU, Randazzo PA, Tessa- Bai L, Lerman MI. Hypoxic repression by motor neurons and microglia. Science rollo L. A kinase-defi cient TrkC receptor of STAT1 and its downstream genes by 312(5778):1389–1392, 2006. isoform activates Arf6-Rac1 signaling a pVHL/HIF-1 target DEC1/STRA13. through the scaffold protein tamalin. J Membrane Transport, Structure, Oncogene 2006 [Epub ahead of print]. Function, and Biogenesis Cell Biol 173(2):291–299, 2006. Gallo SA, Wang W, Rawat SS, Jung G, Singh SR, Zhen W, Zheng Z, Wang H, Transplantation Waring AJ, Cole AM, Lu H, Yan XX, Oh SW, Liu W, Zbar B, Schmidt LS, Sun K, Wilkins DEC, Anver MR, Sayers Daly NL, Craik DJ, Jiang SB, Lehrer Hou SX. The Drosophila homolog of the TJ, Panoskaltsis-Mortari A, Blazar BR, RI, Blumenthal R. theta-defensins human tumor suppressor gene BHD inter- Welniak LA, Murphy WJ. Differential prevent HIV-1 Env-mediated fusion by acts with the JAK-STAT and Dpp signal- effects of proteasome inhibition by bort- binding gp41 and blocking 6-helix bundle ing pathways in regulating male germline ezomib on murine acute graft-versus-host formation. J Biol Chem 281(27):18787– stem cell maintenance. Oncogene 2006 disease (GVHD): delayed administration 18792, 2006. [Epub ahead of print]. of bortezomib results in increased GVHD- Microbiology dependent gastrointestinal toxicity. Blood 106(9):3293–3299, 2005. Ø Palmer S, Boltz V, Martinson N, Mal- darelli F, Gray G, McIntyre J, Mel- lors J, Morris L, Coffi n J. Persistence of nevirapine-resistant HIV-1 in women after single-dose nevirapine therapy for The NCI-Frederick Poster 10 September 2006 Technology Transfer Branch (TTB)

Collaboration Agreements: Gone Are the Days of the Handshake!

Recently, the NCI Technology information) for a period of 3 to should be considered, including Transfer Branch (TTB) received the 5 years. The CA is considered a license rights, funding, the review following question: modifi ed MTA and can be as easy process, time to execution, and types to initiate as an MTA, allowing you of collaborating parties. See the table “Is there some way to document to begin your research quickly. To below for a comparison of these an ongoing collaboration in which execute, the CA requires only an signifi cant issues. materials will be shipped back and authorized signature from the NCI- forth without having to get a signature TTB offi ce, similar to the MTA. Is a CA the Right Mechanism each time something is sent?” Unlike the MTA, the CA requires for Your Collaboration? a research plan that delineates Here are several situations in which With the advent of technology transfer, the anticipated responsibilities of the CA has been successfully applied: a collaboration being conducted with the parties. Furthermore, the CA a nod and a handshake is not always anticipates transfer of additional • A long-standing scientifi c advantageous. At times, there is a research materials consistent with the relationship needs to be documented security in having written, guiding research plan and provides for such so that the exchange of materials principles that are agreed to by both transfers to be made with a simple and data may be managed. parties when collaborating, even with an e-mail, fax, or memo between the academic or government institution. parties describing the materials being • A collaborator on a project sends A Simple Letter Agreement (SLA) transferred and referencing the CA. materials to the other party. The or a Material Transfer Agreement A copy of this communication is then receiving party modifi es the (MTA) provides guidelines for forwarded to TTB for the offi cial fi le. materials and transfers the new transferring material in one direction. No additional signatures are required. materials back to the original Until recently, the Cooperative sending party. Research and Development Agreement How Does the CA Compare (CRADA) was the only mechanism with a CRADA? • A postdoctoral fellow or other for transferring materials back and When deciding whether to enter into laboratory staff member leaves for forth between NCI investigators and a CRADA or a CA, several aspects continued on page 15 their collaborators without generating and signing a separate agreement for CRADA or CA: What You Should Consider each transfer. However, a CRADA is Consideration CRADA CA not a suitable mechanism to document License rights Conveys to the collaborator Does not include licensing some collaborations. fi rst right to an exclusive terms, but collaborator may To foster team science and license to any inventions apply for a license following collaboration, the NCI TTB recently made under the research the standard government developed the Collaboration plan. licensing regulations. Agreement (CA), an instrument that Funding Collaborator may provide No fi nancials may be eases much of the administrative funds to the NCI laboratory involved. burden of these arrangements. The in support of the research. CA defi nes guiding principles through Review process Requires review by the NIH Requires TTB review and terms that serve to protect the interest CRADA subcommittee, signature only. of NCI and the NCI investigator followed by signatures involved in the collaboration. from NCI director’s offi ce and several NIH CRADA How Does the CA Compare subcommittee members. with an MTA? Time to execute 3 to 8 months Similar to an MTA or CDA The CA combines the terms of an (typically 1 to 4 weeks) MTA with those of the Confi dential Collaborator For-profi t entity (company) For-profi t entity (company); Disclosure Agreement (which is non-profi t entity (academic used for the exchange of confi dential or government institution)

The NCI-Frederick Poster 11 September 2006 Environment, Health, and Safety Program

Fitness Challenge 2006 Fitness Challenge Update Events This Fall

The Fitness Challenge has grown! Get inspired by one of the events As of July 1, the rest of the Fort presented this fall, during the last Detrick community joined NCI- quarter of the Fitness Challenge. Frederick in pursuit of a healthier Unless otherwise noted, events are in workforce, and the competition is the auditorium in Building 549, from fi erce. Fort Detrick’s mission partners 12:00 to 1:00 p.m. Bring your lunch are confi dent that they can accomplish and enjoy! their fi tness goals in half the time. So, let’s show them what we’re made of! October 4: Fort Detrick Community Expo & Health Fair Enough Miles for World Travel OHS, the Scientifi c Library, and the Over the past nine months we’ve Cancer Information Service will been striving to walk, bike, and run join lots of other exhibitors at this around the world (approximately installation-wide event. 25,000 miles). So far, we’ve traveled Dr. Larry Arthur is an active 11:30 a.m. – 2:30 p.m. nearly 16,000 miles, enough to proponent of the Fitness Challenge. Odom Fitness Center follow NCI-Frederick scientists as they participated in clinical trials, October 12: How to Involve the conferences, and gathered samples Check Out a Fitness Video Family with Exercise and Nutrition for research throughout Japan, South Presented by Occupational Health Korea, China, Vietnam, Cambodia, Many new fi tness DVDs have Services Singapore, and South Africa. We are been added to the Center for Health quickly racking up enough miles to Information’s video collection in October 15: Diabetes 5K Walk in move from South Africa to Botswana, Building 549. Stop by to borrow a Baker Park where our colleagues have actually video and try some new exercises. Start Time 1:00 p.m. captured wild lions to obtain research You’ll fi nd videos for all types of Baker Park Bandshell samples! From there, we’ll head exercise: low-impact aerobics, walking For more information go to northwest to visit our friends in Mali away the pounds, tai-chi, pilates, and http://walk.diabetes.org or call before heading back to Frederick for yoga, as well as strength training for 888-DIABETES our grand fi nale on December 21. all fi tness levels. Ø It’s Not Just about You If you haven’t been entering your Four Months to Go! weight loss, miles, or exercise hours Here’s how we’re doing as a group. All information is based on data entered on the Fitness Tracker, now’s the time into the Fitness Tracker as of September 5. We’ve made some signifi cant to get started! If you are behind, or progress in our miles biked, run, and walked, as well as in our “other” missed a few days of entering your category. We still have a ways to go with the weight loss. So for the next four data, you can always go back and months, let’s just say “No!” to that dessert! update the tracker. Remember, every Average Per Percentage Activity Total Goal pound, mile, or hour counts toward Person* Accomplished your personal goals. Just as important, however, is your organization’s Pounds lost 5 756 2,000 38% total. Remember, the operative word Miles biked 105 4,633 is “challenge,” and we have been Miles run 62 3,748 challenged to see which organization Miles walked 60 7,466 can lose the most. Total, bike, run, walk 15,847 25,000 63% Hours Other 68 7,339 8,760 84%

The NCI-Frederick Poster 12 September 2006 Environment, Health, and Safety Program

November 9: Stress during the How Is Your Organization Doing? Holidays with guest speaker Selden Cooper, Employee Assistance Program Coordinator

December 21: Fitness Challenge 2006 Finale This is it – the moment of truth. Be sure to attend to fi nd out the fi nal results and celebrate our success!

Share Your Success! If you have a Fitness Challenge success story you’d like to share, OHS would love to hear it! Call 301-846- 1096, or email: [email protected]. Ø Remember to enter your results! Ø

PALS

PALS Art Auction

Part of Take Your Child to Work Day was an art auction held at the day care center, Play and Learning Station, PALS. With the art on exhibit in the 549 Conference areas, people could inspect fi nger paintings and sponge paintings ranging from bright yellow fi sh to the American fl ag, montages of bright colored papers in triangles, squares and rectangles, as well as red, blue, and brown footprints. Many of the paintings would give Jackson Pollack a run for his money! This year, the auction raised nearly $270. Monies raised will be used for materials needed for the day care center. Ø

The NCI-Frederick Poster 13 September 2006 Environment, Health, and Safety Program

NCI-Frederick: For example, according to boxes from labs and grind them on- Environmentally Friendly Environmental Safety Offi cer Paul site for pick-up by a local recycling Stokely, from September 2004 to fi rm. The processed resins from these A researcher in the middle of a September 2005, 108 containers of plastics are used to make plastic coat procedure realizes she needs 20 chemicals were placed in safe storage hangers, fl ower pots, and other general milliliters of concentrated sulfuric acid and 57 of them were re-issued, at no consumer items. to make up a reagent. There is none cost, to laboratories and shops at the in her lab, and no adjacent labs use NCI-Frederick campus. This resulted in Recycling Benefi ts concentrated sulfuric acid. What can a cost savings of approximately $2,912. Children’s Inn at NIH she do? You can arrange for Waste General Recycling Management to pick up Tyvek Chemical Recycling NCI-Frederick enjoys many benefi ts garments from clean rooms and Fortunately, NCI-Frederick operates from working within Fort Detrick, production labs. These garments a Chemical Surplus Program to collect including participation in their recycling are washed and re-used by other and store unopened bottles of shelf- program. Every week, the United States industries; for every suit recycled, durable chemicals such as solvents, Army Garrison (USAG) collects offi ce $0.25 is donated to Children’s Inn at acids, bases, salts, and specialized paper from several dozen areas around NIH to help juvenile cancer patients. cleaning supplies. Surplus chemicals the NCI-Frederick campus. Ink and toner cartridges from printers are advertised on the NCI-Frederick The USAG also provides recycling and copiers may be dropped off in Web site by the Environment, Health, containers for glass, plastic bottles, specially marked boxes found in and Safety (EHS) Waste Management aluminum, cardboard, newspapers, many offi ces. When these boxes are offi ce (see box on page 17). Using this and more (see box for information on nearly full, Waste Management will Web site, our researcher fi nds that a where to fi nd these drop-off points). pick them up. Proceeds from these 100-mL bottle of concentrated sulfuric Many lunchrooms have containers for cartridges also go to Children’s Inn. acid is available. She makes a quick empty plastic bottles and aluminum phone call to Waste Management, and cans, and we are fortunate that staff It Doesn’t Stop There the sulfuric acid Other items that may be recycled is delivered the include: same day. Where does • lead acid batteries from computer EHS get surplus backup systems and shop chemicals? equipment Unused • X-ray and photographic fi lm and chemicals are paper frequently • metal gas cylinders and lecture obtained bottles whenever a • fl uorescent light tubes lab moves, is • lead shielding renovated, or • shrink wrap cleaned out. Also, many Technician surveys chemicals available to Check the Web site (see box on labs purchase researchers through the Chemical Surplus Program. next page) for the complete list of chemicals recyclable items. in bulk because they are less members volunteer to deliver these expensive; unfortunately, most of items to the drop-off points. If you have any questions or the chemicals will expire before they comments about NCI-Frederick can be completely used. However, Plastic Pipette Trays recycling or other environmental efforts, if the labs send what they don’t—or Disposable pipette tips are packaged please call the Waste Management can’t— use to the Chemical Surplus in trays or boxes made of low-density offi ce, at 301-846-5718. Ø Program, such waste can be reduced or polyethylene (#5) plastic. The Waste even eliminated. Management offi ce will collect these

The NCI-Frederick Poster 14 September 2006 Environment, Health, and Safety Program

Questions about Recycling? Environmental Accountability Mandated General Recycling: by Executive Order http://home.ncifcrf.gov/ehs/recycling/ This Web site provides information on over 20 types of items that can be Executive Order 13148, issued recycled at NCI-Frederick, and how to package your recyclables for pick-up. by President Clinton in 2000 and You’ll even fi nd an interactive map showing the Ft. Detrick drop-off points endorsed by President Bush in by type of material. 2004, mandates that government facilities develop recycling Surplus Chemicals: programs and reduce waste http://home.ncifcrf.gov/ehs/ehs.asp?id=79 wherever practical. NCI-Frederick This Web site provides information on how to recycle chemicals or to order developed its environmentally chemicals from the list of those currently in surplus. friendly programs in response to this mandate and encourages General questions, or to arrange for pick-up of recyclables: everyone’s participation. Ø Call the Waste Management Offi ce, 301-846-5718. Ø

Where Are They Now? continued from page 11 a new position but will continue Dr. Lori Bernstein to work collaboratively with NCI. Materials and confi dential Graduate student, 1987–1992; post-doctoral fellow, 1995–1996 information will be shared.

Dr. Lori Bernstein worked fi rst and mRNA expression, and mediate • Preliminary studies are needed in Dr. Nancy Colburn’s laboratory. neoplastic transformation” (Samuel, to decide if a CRADA is the next While doing post-doctoral work et al. Biochem J 388(Pt. 3):921-928, step in the development of the at NCI-Frederick in Dr. Scott 2005), she wrote. technology. Durum’s laboratory, Dr. Bernstein Currently, she is an assistant found “that AP-1 activation occurs professor in the Molecular and Cellular • Multiple NCI investigators are in cells susceptible to oncogenic Medicine Department, College of receiving compounds to be used as transformation but not in cells Medicine, Texas A & M University. screening agents against multiple resistant to oncogenic transformation” Dr. Bernstein said that Dr. Colburn disease targets, and the collaborator (Bernstein and Colburn, Science “was and continues to be an excellent will supply derivatives of compounds 244[4904]:566-569, 1989), according and dedicated graduate mentor and as a result of the initial hit. to a recent e-mail she sent in response scientists. I am grateful for having to our questions. Reminiscing, Dr. known her and worked with her.” Ø • The NCI investigator wants to Bernstein noted that her tenure at NCI- memorialize or capture technology Frederick was during the Persian Gulf transfer activities for reporting war, and she remembers that requirements (site visits or awards, there was a “big increase in for example). security at the gates.” Since leaving NCI- If the CA sounds like something that Frederick, she has you may be interested in using, or if continued her research you have any questions related to this and has identifi ed “new or any other technology transfer topic, AP-1 DNA binding please do not hesitate to contact TTB proteins that repress AP-1 at 301-846-5465 or on-line at http\\: target gene transactivation www. ttb.nci.nih.gov. Ø

The NCI-Frederick Poster 15 September 2006 Poster Puzzler What is it? Where is it?

Your challenge, should you decide to accept it, is to correctly identify the item and its location from the picture to the right. Clue: It’s somewhere at Fort Detrick/NCI-Frederick. Win a framed photograph of the Poster Puzzler by e-mailing your guess, along with your name, e-mail address, and daytime phone number, to Poster Puzzler at poster@ncifcrf. gov. Alternatively, you can send us your guess, along with your name and daytime phone number on one of The Poster forms found on the front of The Poster stands in the lobbies of Buildings 426 and 549. All entries must be received by Friday, October 27, 2006, and the winner will be drawn from all correct answers received by that date.

Good luck and good hunting! Ø

The Poster Puzzler: What Looks Like “Down” May Be “Up”

The June Puzzler is one of the lights in the overhang in front of Building 459. These lights are not nearly as old as the building, which was originally constructed in 1944 as a cold storage facility for the Biological Warfare Program. In the 1950s, it was renovated into an animal holding facility, which operated until 1974, when the Army modifi ed it again to house chemistry and microbiological laboratories. In 1993, the building was turned over to the National Cancer Institute. Renovations completed in 1996 transformed a 50- year-old laboratory building into a state-of-the-art cGMP production facility for the Monoclonal Antibody Recombinant Protein Production program—and included the overhang with lights. Today the building is part of the Biopharmaceutical Development Program.

Did we mention the photo was upside down?

Editor’s Note: June’s Poster Puzzler was a test of everyone’s powers of observation. Our designer shamelessly manipulated the photo by fl ipping it upside down, to increase the diffi culty of fi nding it. Luckily, we have lots of keen observers in our community: we had more correct entries for this photo than for any other since our Puzzler feature began. Congratulations to our June 2006 Puzzler winner: Wade Schirmer, Contracting Thanks to all the participants in the June 2006 Poster Puzzler! Offi cer’s Technical Representative, Facilities And special thanks to Rocky Follin, FME, for the information in Maintenance and Engineering. Ø this article. Ø

The NCI-Frederick Poster 16 September 2006 Poster People Profi le: Peter Boving

Peter Boving, Fire Prevention a burden.” It was then that he began and lives, he said, “Fire prevention is Inspector at SAIC-Frederick, Inc., is “looking for a different way to serve something I understand and respect.” no stranger to the scientifi c research the research community.” environment. His grandfather Accepting a position as Fire Active Participant in the came from Denmark to work at Prevention Inspector represented NCI-Frederick Community the Smithsonian Institute as an an exciting change in direction A former substitute teacher, Mr. entomologist, and his father’s career and a chance to return to his roots. Boving enjoys the Elementary was in medical research. “Even if Outreach Program because I didn’t follow their footsteps to a “teaching science to second career in science, a research campus graders is a wonderful way feels like home,” he commented. to contribute to the future.” NCI-Frederick has been Mr. His favorite committee is the Boving’s “home” for the last 19 years. Employee Diversity Committee When he began in 1987 as Manager because, he said, “my main interest of Protective Services, he brought a is the celebration of diversity commitment to helping others that through cultural events. I enjoy stretched back to early adulthood. international cooking, and wish During his undergraduate years at to bring interesting cultures, Albion College in Michigan, he was a languages, music, and foods to Campus Service Offi cer, volunteered NCI-Frederick.” with the Albion Ambulance Service, and trained as an Emergency “Our Roles Overlap” Medical Technician. What Mr. Boving likes best about After college, he worked as a mall working at NCI-Frederick is the Security Sergeant in Detroit; managed variety: of work of experiences security departments at Trinity with the Employee Diversity Team, College, Washington, D.C., then and of friendships he has made at Mount Saint Mary’s University, Peter Boving here. He commented, “Our roles Emmitsburg, MD; and earned a Fire Prevention Inspector overlap: family, work, friends, and master’s degree in criminal justice, the at SAIC-Frederick, Inc. volunteering,” adding that NCI- fi rst of two graduate degrees. Frederick “understands and supports While managing the Protective the interaction.” Services Department here, he earned Becoming a Certifi ed Fire Protection Variety clearly extends into other his second master’s degree, this time Specialist “was not a chore for me,” aspects of his life. His natural curiosity in computer science. The scientifi c he recalled, “because I’d had a wide has generated a diversity of interests, alarm call-in information used to be variety of training in fi refi ghting including cooking, camping, hiking, “hand-written on scrap paper,” he techniques, SCBA [self-contained cross-country skiing, and traveling. said. “The name and phone number, breathing apparatus], rescue, pumps, When he can’t travel, he said, he if any, were often out of date.” With rappelling, aerial ladders, sprinkler watches the world on satellite TV or call-in information collected in a systems, incident command, listens to it via amateur radio. “I like database, he continued, “we were hazardous materials response, arson to know what’s going on,” he said. able to print updated call-in cards, investigation, NFPA [National Fire Mr. Boving draws inspiration from and comprehensive reports before Prevention Association] codes and, of a statement made by Margaret Meade each weekend.” course, fi re prevention.” when she spoke at his college many He has provided fi re extinguisher years ago: “Never doubt that a small New Position Brings training at a vocational high school, group of thoughtful, committed people Him Full Circle given fi re prevention talks in can change the world. Indeed, it is The events of September 11, 2001, elementary schools, and inspected the only thing that ever has.” With his profoundly affected Mr. Boving. hundreds of establishments and natural curiosity and willingness to “On that day,” he said, “the weight houses. Having seen fi rst-hand the embrace diversity, he can certainly be of protecting 3,000 people became damage fi re can do to both buildings counted among this group. Ø

The NCI-Frederick Poster 17 September 2006 FME Machine Shop: One-of-a-Kind

chambers sized like the old ones, so Working closely with researchers and she could continue to use the trays and technical staff in the AIDS Vaccine combs. Doing that saved lots of money Program and the Gene Expression for her laboratory—and by extension, Laboratory, Mr. Crone designed an for NCI-Frederick. acrylic box anchored with rubber feet to hold centrifuge tubes and dialysis In a Hurry? chambers in place. A narrow vertical The Machine Shop fellows have also slit in the box, the length of the test made items that would have taken tube, enables the researcher to insert weeks or months from commercial a needle at any point to withdraw venues. For example, they made a fan a sample. No worries about needle shaft for Charles River Laboratories’ sticks or spills. Animal Facility and a steam coil for Rodman Smith, Biological Products the Fermentation facility. The fan Laboratory of the AIDS Vaccine shaft alone would have taken about Program, needed a specialized six weeks for delivery, while shop support tile for his robotic, computer- fabrication took only eight hours. controlled Packard MultiProbe II to The steam condensing coil was hold larger test tube racks. When the Look around your offi ce, your estimated at 12 weeks for delivery manufacturer was unable to provide laboratory. Chances are, you have and to cost $3,000. The machinists one that suited his needs, the Machine more than one indispensable item made it in eight hours for about $100 Shop made one that lets Mr. Smith that was manufactured right here, in materials, according to Max Reed, process more samples simultaneously, whether it’s a special hood, a repair Machine Shop supervisor. or replacement for an item no longer When Glenn Hegamyer, Laboratory made, or a unique product for a special of Cancer Prevention, was preparing research need. sucrose gradients for polysome Aluminum, stainless steel, acrylic, fractionations, the gradient block nylon—Facilities Maintenance broke. He would have had to discard and Engineering’s (FME’s) master his samples, if not for the quick in- machinists—Tom Crone and Jimmy house repair that Mr. Crone and Mr. Notnagle—work with all kinds of Notnagle performed. materials on all sorts of projects. For example, when Dr. Mi Li, Inventiveness Macromolecular Crystallography Mr. Crone and Mr. Notnagle have Laboratory, needed a transition table created low-radiation and splash for his X-ray diffractmeter, X-ray shields out of thick Plexiglas. enclosures for his X-ray generators, They’ve made 96-hole test tube racks and a stand for his optics, the two numbered across the top and lettered saved him time and money by careful down the side so that it’s easy to planning, good craftsmanship, and identify each test tube by position. quick delivery. “The devices work And they crafted an aluminum box very well with the other parts of my that could be plunged into ice to chill thus saving him time in setup and data collection system. Tom and Jim cells much more quickly than the money for materials. did very good job for me and I really commercially available plastic. Dr. Shizuko Sei, Viral Vector appreciate it,” Dr. Li said. A few years ago, they experimented Toxicology Laboratory, faced a similar Dr. Lidia Hernandez, Cancer and with several whale dart prototypes problem with the Tecan auto-robotic Developmental Biology Laboratory, for a research scientist. “This was system in her former laboratory, had many trays and combs for her uncharted territory,” Mr. Crone the Laboratory of Antiviral Drug electrophoresis gel chambers, but they said, chuckling. Darts and scientist Mechanisms. She needed microplate wouldn’t fi t the new, differently sized were featured in the July 1999 versions. The Machine Shop made National Geographic. continued on page 19

The NCI-Frederick Poster 18 September 2006 Campus Improvement Committee

Campus Improvement: Identity Program Nears Completion

The Campus Improvement Committee continues to make progress on its goal of strengthening the identity of NCI-Frederick on the Ft. Detrick campus. Banners identifying the NCI-Frederick boundaries were installed in 2005, and installation of new building signs was completed earlier this year. The current phase of the identity program is the installation of new directional signs, which began in August and is expected to be complete this fall. Ken Michaels, Signage Committee Chair, is pleased with the way the project is turning out. “We’ve been happy with the outcome of this project,” he said, “because not only does it identify NCI-Frederick, but it also gives people here a boost to be reminded that they are part of a larger entity.” Look for more projects from the Campus Improvement Committee in the coming months. If you would like to participate or have a suggestion for the committee, you may submit your comments or ideas through our Web site, http://web.ncifcrf.gov/campus/ committees/campus.asp. Ø

continued from page 18 carriers properly set to hold tissue attached to a pulley that opened and well as challenging. About two years culture plates with infectious agents closed very smoothly and gently, for ago, they made a kind of “swimming for the automation program. After acoustic isolation and to minimize pool”—a 36” x 40” tank with consultation with Dr. Sei, Mr. Crone dust particulate. This was done for Plexiglas stands at different levels— built a prototype carrier system, Dr. Anil Patri, Nanotechnology for behavioral research on mice. which greatly helped the automation Characterization Laboratory, who Another time, they made Plexiglas project get started. The manufacturing studies nanomaterial using an atomic puzzles for monkeys who had to learn company’s representative was force microscope (AFM). The AFM to use a rod to dig out a treat such as impressed with Mr. Crone’s design is highly sensitive to particles, peanut butter and to tilt the puzzles a and gave it high marks. sound, and vibrations. The hood certain way for other treats to drop out. For several scientists, Mr. Crone and was placed on a marble bench with “There’s something different to do all Mr. Notnagle have created probably active vibration isolation to facilitate the time,” Mr. Notnagle commented. their largest projects: biosafety level sensitive measurements at the “It’s always challenging and hoods. For example, last spring the nanometer level with greater accuracy interesting.” Ø machinists made a self-contained, and precision, Dr. Patri explained. 45-inch-high hood with a rolling door Some projects have been fun as

The NCI-Frederick Poster 19 September 2006 Outreach and Special Programs

Take Your Child to It was neat for the kids to speak their children and their host families turned Work Day Even More own language with Russian scientists, out to be the most time-consuming Multicultural than Usual and to have this experience.” problem. Even for the Take Your Child He said that the program organizer, to Work Day events, a couple of the Jerry Burge is a senior research Barbara Birnman, was “very gracious children were unable to attend, due to technician in the AIDS Monitoring in allowing us to add the kids to the other commitments with their families. Laboratory, Clinical Services Program, programs. It was such a nice thing This group was a little different from Applied/Developmental Research for her to include the kids. She didn’t the usual summer exchange program, Directorate. His main job is to process hesitate a moment,” he said. Even in that these are needy children. and store blood on HIV-positive the chaperone “had a great time; just Belarusse is in the heart of where the patients that are on drug studies at hearing what was going on and getting radiation was the strongest after the NIH. He also runs whole-blood Chernobyl nuclear plant accident; staining assays and ELISA assays, medical reports say that if children and stores blood products and in their developmental years can samples for future testing. But for be brought out of that area for at six weeks this summer he was a least six weeks at a time, they can “father” to a Belarusian child, in gain a year to their lives for each addition to three of his own (his 6-week period. wife gave birth to their fourth Thus, the American-Belarussian three weeks after the Belarusian Relief Organization (ABRO) children returned home). Seven- requires host families to set up year-old Lira was one of a group appointments with local dentists, of fi ve Belarusian children who optometrists, and family doctors visited the Frederick area. for basic exams. “We were Belarus is a small, Eastern European to speak with other Russians.” He expecting a sickly child, and we were country which gained its independence said that the chaperone was herself a blessed because Lira never got sick after the dissolution of the Soviet doctor—a pediatrician—in Belarus. while she was here,” Mr. Burge said. Union in 1991. Belarus, whose capital The children participated in When Lira arrived, she weighed 48 is Minsk, has a population of a little “Unlocking the Mysteries of the pounds, only eight pounds more than over 10 million in an approximate Brain” and “Fish and Frogs.” The Mr. Burge’s four-year-old daughter. 80,200 square mile area, according to kids got to make slime in one of the She weighed 63 pounds when she left. the Encarta Encyclopedia at http:// sessions, and Lira kept her slime until Host families pay for everything encarta.msn.com/encyclopedia_ she returned to Belarus. for the children, including air fare. 761553191_2/Belarus.html#s10. Mr. Burge had high praise for the However, the doctors involved did all One of the highlights of the program’s organization and those who their work pro bono, even to giving children’s visit was participating in hosted the sessions. “People were very Lira $400 glasses, and another child, Take Your Child to Work Day. This kind and patient with the interpreter braces (also arranging for follow-up special program is always a bit wild having to speak. They [the Russians] orthodontia to take with her to Belarus). and crazy at NCI-Frederick. For one really appreciated that.” “ABRO tries to match kids with thing, it happens in July, rather than This was the fi rst time that Mr. Burge families that have similar cultural April, as is done nationally. Having a and his wife had hosted a foreign backgrounds—city kids with city July date allows us to have numerous guest. He counted it as a “wonderful families, country kids with country activities outside, to get participation experience.” He and his wife had families, so that it won’t be quite so from children whose parents are been most nervous about the language much of a cultural shock when they understandably reluctant to take them barrier; however, both the American come here,” Mr. Burge explained. He out of school at other times of the families and their Russian “children” and his wife hope to host Lira again year, and to enjoy the more relaxed had English/Russian dictionaries, and next year, if her mother will allow summer atmosphere. the chaperone was always available her to return. “That would just be The children “really enjoyed it,” by phone. phenomenal.” And Mr. Burge hopes to Mr. Burge said, “because of the More than language barriers, visit Belarus next year. Ø multicultural aspects of being on base. scheduling events for the fi ve Russian

The NCI-Frederick Poster 20 September 2006 Reporting In

Librarians Prove do.” He was visibly moved by being in Guard troops standing watch over New Orleans IS Ready the Convention Center, which he noted fl attened and severely damaged homes for Visitors had “few markers of what transpired,” was a sobering experience. since he had last been there in the In one unique, delightful speaker --Debby McCalpin, Library Director, midst of the crisis. session, author/musician Tom Scientifi c Library I have always loved being a librarian, Sancton read from his book, Song for but I have never before experienced My Fathers: A New Orleans Story Editor’s note: Debby McCalpin, Library such an outpouring of gratitude from in Black and White. He alternated Director, was one of more than 17,000 people who were so appreciative of anecdotes about growing up around librarians who attended June’s annual our contributions. It was not unusual to jazz with performances of 1950s New American Library Association (ALA) meeting be greeted on the street with comments Orleans-style jazz with his ensemble. in New Orleans, where librarians not only attended the fi rst major conference held in New such as “Are you with the librarians? The session, in just 90 minutes, Orleans since the 2005 hurricanes, but also Thanks for coming,” or to see t-shirts beautifully portrayed the rich cultural helped renovate and restore several libraries in in stores with a “Librarians Rule!” traditions and artistic talent that live the region. imprint. We heard from many residents on in New Orleans despite the damage who fear they may lose their unique from Katrina. The American Library Association’s and vibrant culture if the economy ALA leaders said the Katrina (ALA) June 22–28 Annual Conference can’t support it. We learned that many tragedy demonstrated nationwide in New Orleans, with an estimated former residents have been unable to the critical role public libraries can 17,000 attendees, was the largest return, either because their homes have play in a disaster. Libraries across the convention in the city since Hurricanes been destroyed, or because they cannot country have offered Internet access Katrina and Rita had devastated the afford the housing that does exist, to displaced residents desperate for region in August and September 2005. due to skyrocketing rents. In fact, information. As residents returned As noted in the June 22, 2006, issue many employers, in order to re-start home, local libraries gave people a of Library Journal, more than 100 and maintain their businesses, were place to fi nd housing, look for jobs, libraries in Louisiana were destroyed housing their own employees. and share information. While FEMA’s or damaged, and 40 remain closed. We learned about some of the (Federal Emergency Management Conference speakers said that ALA complex issues facing the rebuilding Agency’s) policy manual neglects to attendance had truly made a difference of New Orleans. Universities in list libraries among essential services for New Orleans, not only because of the area, such as Tulane, University after a disaster, an ALA administrator the economic relief we were providing of New Orleans, and Loyola, are in charge of coordinating volunteers to the city, but also because over 1,000 concerned about attracting students to noted, “Libraries are kind of the volunteers, including vendors from New Orleans again. Will students and heart of the communities. They’re the exhibit hall as well as conference faculty return? Will developers and a cornerstone, a building block to participants, helped renovate and opportunists rebuild without concern community service, to democracy.” restore local libraries, some of which for architectural integrity? Will New Madeleine Albright, the keynote still contained over a foot of water just Orleans culture be lost in the transition speaker at the opening general three months before the conference. the city is undergoing? session, congratulated librarians for Major library vendors donated My hotel, and others in the French keeping the faith with the people of electronic resources to academic Quarter, did not appear to be New Orleans. She called libraries libraries at Tulane, LSU, Loyola, and shorthanded, and it was clear that the “the laboratory of freedom” and “the many other institutions, and thousands hospitality industry’s effort to create biggest bargain on the face of the of new books. an area for visitors that was separate earth.” Incoming ALA President-elect “You are pioneers, and you are from the destruction was successful. Leslie Burger commented that she sending a signal to the world that says The French Quarter businesses and envisioned a world in which no one New Orleans is okay to visit,” Mayor restaurants were busy and the area will ask, “Do we still need a library?” Ray Nagin told us at the opening beautiful and so full of fascinating and called upon colleagues to join session. Keynote speaker Anderson history. But many of us also felt her “in a world in which libraries Cooper thanked librarians for “once compelled to see some of the more transform their communities.” Well again leading the way, as librarians devastated areas. The sight of National said, indeed. Ø

The NCI-Frederick Poster 21 September 2006 Farmers’ Market

Who’s Behind the Counter? have been raising bees for 10 years and are very knowledgeable about Each Tuesday, June through October, what makes one honey distinct from you probably visit the Fort Detrick another, and, of course, about the Farmers’ Market in front of Building bees—mostly Italian—they raise on 549, selecting from the array of their farm near Creagerstown, MD. To vegetables, fruits, pastries, muffi ns extract the honey, they lift the frames and breads, honey, fl owers and herbs, of honeycomb out of the hive and cut lotions and emu meat, milk, yogurt and off the wax cappings, centrifuge the cheeses. You exchange pleasantries frame, removing the honey; strain with the friendly faces behind the resulting honey through a fi ne the counters—but have you ever screen; and fi nally, bottle the raw, wondered what’s involved in getting unprocessed and unpasteurized honey. these products to you? Recently, The beekeepers can be reached at 301- when we talked with these vendors, 898-7823; or through their Web site at we discovered that most are either Frugalbee.com. certifi ed organic or follow organic At the Lewis Orchards truck and practices—so you can be sure you are tent, you can fi nd both white and getting very wholesome food here. yellow peaches, Shiro and Methly Rudy and Judy Medicus run Cats’ plums, blueberries, cherries, numerous Paw, a certifi ed organic farm near varieties of apples, and sweet Union Bridge. At their Farmers’ corn. The farm near Cavetown in Market tent, you’ll often fi nd Morning neighboring Washington County has Glory bread, muffi ns, and cookies; been in the family for six generations, and fresh produce, such as lettuces, with teens of the latest generation now squashes, and tomatoes, and potatoes. helping to sell their sweet fruits and With a PhD in biochemistry, Dr. vegetables. For information, call Mrs. Medicus was a “natural” to become Shirley Lewis at the family-run fruit certifi ed as an organic farmer. Dr. stand, 301-824-2811. Medicus is so rigorous in his organic Janet Madsen, of Janet’s Cut practice that he doesn’t even use the Flowers, is a master gardener. She’s Neem bark extract (Azadirachtica been literally growing her business indica), a weed killer that many other for the past fi ve years on a 10-acre organic farms employ. “It’s also a very farm–including annuals, perennials, powerful liver toxin,” he said. Nor herbs, and native plants. During a Stone Hearth Bakery has been does he use even approved pesticides; recent market day, she was selling selling at the NCI-Frederick Farmers’ instead, he handpicks “potato beetles annuals such as sunfl owers, white Market since the market started in off the plants each day, 50 or more liatria, gladiolas, snapdragons, 1998. “The only time we missed was each day,” he said. The Medicuses can cosmos, forget-me-nots, zinnias, and 9/11, and that was only because the be reached at 410-775-2819. feathery coxcomb; the biennial sweet gates were closed when we arrived At the Frugalbee, you’ll see William; perennials, including grasses, and we couldn’t get in,” Elizabeth several varieties of honey, as well as gooseneck loosestrife, blue salvia, Oluich said. She said they started the beeswax candles, photo notecards, dahlias, and lisianthus; and herbs, bakery on East Street in 1993 because and vegetables—okra, shallots, leeks, including catnip, rosemary, lemon her husband was a chef and wanted beets, onion, soybeans, potatoes, verbena, lavender, hyssop, parsley, and his own bakery. “Eventually, all chefs and heirloom beans such as Dragon basil. Ms. Madsen sells her plants at want their own bakery,” she said, Tongue. Although not certifi ed various farmers’ markets and arts and smiling. Immediately, they were asked organic, these beekeepers don’t use crafts shows, especially in the spring to join the Baughman’s Lane farmers’ any sprays or chemicals on their crops. when, she said, people are particularly market, also in its fi rst year. Their The beekeepers, who prefer to be anxious to buy garden materials. wares include many kinds of breads known simply as Bruce and Kathy, and rolls, croissants, scones, and

The NCI-Frederick Poster 22 September 2006 Farmers’ Market buchtels (a pastry fi lled with apricots pets; however, the younger ones are products after October 31, when and nuts). Mrs. Oluich said that she raised for meat. Each bird provides our market closes for the 2006 likes the NCI-Frederick location and only about two pounds of fi let and a season, check their Web site for the loyal customers—“We see a lot of little more ground meat. Mr. Royer information on home delivery at www. the same people every week,” she said. sells both patties and fi lets in frozen southmountaincreamery.com. Jim and Peggy Royer have about packages, all USDA-inspected. Nearly New to NCI-Frederick this year 50 emus on their farm, Old Orchard every part of the emu is useful. Mr. is the Truffl e King (Imperial Emu. Members of the ostrich family, Royer explained that a layer of fat on Chocolate Company) However, emus stand about four feet tall at the birds’ backs is rendered into emu Tim Miller isn’t new to chocolate. A their backs and about six feet tall at oils, lotions, soaps, and lip balms. The self-confessed chocoholic, Mr. Miller their heads. Like prized cattle and feathers are popular for fi shermen’s likes to cook; and after seeing a show horses, the breeders have become fl y ties and for crafts. The eggs are on making chocolates, he spent two nearly as large as ostrich eggs and are years studying and experimenting a beautiful, intensely dark green. Each with techniques and fl avors until he egg is the equivalent of 6 chicken perfected his method. For the past eggs, according to Mr. Royer. The three years, Mr. Miller has been emus lay their eggs in Australia’s making melt-in-your-mouth truffl es at summer, which unfortunately for the Clustered Spires, a licensed bakery them is our winter. They’re unable to on Montevue Lane. Each month, he adjust their biological clocks to the produces a special fl avor; other fl avors different hemisphere, Mr. Royer said, must be ordered, in increments of at so he incubates those eggs that will least 3 dozen. The chocolates, which become hatchlings. “Emus make great keep 6-8 weeks in the refrigerator pets,” he added. He takes two young or 2-3 weeks at room temperature, ones to a petting zoo at Westminster contain no preservatives. The secret Orchards each summer; by fall, they of Mr. Miller’s chocolate truffl es is are very tame. Mr. Royer can be the gnache, which he forms by hand reached at 301-241-3996 or by e-mail and dips into a very high-quality, rich at [email protected]. Venezuelan chocolate. “Venezuelan In business since 2001, South chocolate has subtle undertones,” he Mountain Creamery, owned by said. Despite this being his fi rst year, Randy and Karen Sowers, focuses he said he has found an enthusiastic on milk and dairy products. As reception and a number of return Maryland’s only on-the-farm buyers. Like many of the vendors here, processing plant, the creamery he also sells at other area markets. makes most of its own cheeses but Besides all these vendors, nearly also carries some from area farmers. every week the market features a local The company says proudly that its crafter. Also, representatives from the herd is grass-fed and is not treated Master Gardeners of Frederick County with growth hormones. While South area there to share gardening tips Mountain Creamery is not certifi ed during the season. organic, the farm considers itself Check out the vendors at the next “all-natural” and follows organic Farmers’ Market; you can be assured certifi cation because, the Sowerses that the foods you are buying are say, “It does not make sense for us wholesome and will make much to spend the money, maintain all of appreciated gifts for family and the paperwork, and deal with the co-workers or a terrifi c lunch just inspectors, to basically do what we for you. The Farmers’ Market 2006 already are doing.” If you can’t get season continues on Tuesdays through to the Tuesday Farmers’ Markets October 31, 11:00 a.m.—1:30 p.m. (or or if you still want their delicious sell-out) in front of Building 549. Ø

The NCI-Frederick Poster 23 September 2006 Frederick Employee Diversity Team

Diversity Team Wins Recognition from NCI of the cultures and history of Native Americans. Its mission is The 2006 Diversity team to collaborate “with the Native gathers in front of the world peoples of the Western Hemisphere map and display case in to protect and foster their cultures the NCI-Frederick Café. by reaffi rming traditions and beliefs, Front, L to R: Debra Dixon, encouraging contemporary artistic Maritta Grau, Sukanya expression, and empowering the Bora, and Ethel Armstrong. Indian voice,” according to its Back, L to R: Paul Miller, Web site, http://www.nmai.si.edu/ chairman; Peter Boving; subpage.cfm?subpage=visitor&seco Dr. Scott Keimig. nd=about&third=about.

The Diversity Team recently received 2. The National Museum of the • The museum’s permanent notice that it has been selected by Dr. American Indian (NMAI) is the exhibitions include: Our Universes, John Niederhuber, Director of NCI, for newest component. a fascinating representation of its 2006 “Leading Diversity Award.” 3. We hope you didn’t say “No.” traditional native cosmology, Paul Miller, chairman of the NCI- NMAI is so close to home (only philosophy and spirituality; Our Frederick Diversity Team, said an hour away) and is a veritable Peoples, events that shaped the that “this is truly an honor and we treasure not to be missed. lives and outlook of native peoples appreciate the recognition.” Fortunately, you can still get from 1491 to the present; Our The current team includes Debra an “A” on this quiz by visiting Lives, insights on the contemporary Dixon, Paul Miller, Ethel Armstrong, NMAI during November, National identities of native peoples in the Peter Boving, Maritta Grau, Scott American Indian Heritage Month. 21st century. Keimig, and Sukanya Bora. Diversity meets the fi rst Thursday of each Care to do a little research before • Over 3,500 items in the Window month at 9:00 a.m. in the Building 538 your visit? A rich trove of pictures and on Collections Gallery illustrate the conference room. If you are interested information is available at the NMAI remarkable breadth and diversity of in joining the team, call chairman Paul Web site, http://www.nmai.si.edu/ historical Native American objects. Miller at 301-846-5660, or come to index.cfm. any upcoming meeting. Here are some study points: The National Museum of the American Indian, Washington, D.C., • The fi rst “American Indian Day” is located on the National Mall Perfect Fall Visit--NMAI was declared by the state of New between the National Air & Space Pop-Quiz Time: York in 1916, but it wasn’t until Museum and the U.S. Capitol Building 1990 that President George H. W. at the intersection of 4th St. and 1. How many museums comprise the Bush declared the fi rst National Independence Ave., S.W. Its hours are Smithsonian Institution? American Indian Heritage Month. 10:00 a.m. to 5:30 p.m. daily. 2. What is the name of the newest His action was based on legislation museum? presented by Senator Daniel Inouye EDT MOVIES Have 3. Have you visited this museum? (D-Hawaii) and Congressional Circulated 1,592 Times! Delegate Eni Faleomavaega According to Ethel Armstrong and (D-American Samoa). National Martha Summers, Scientifi c Library, Answers: American Indian Heritage Month 13 of the 46 EDT movies have been 1. Sixteen museums comprise the honors and recognizes the original checked out 50 or more times this past Smithsonian Institution (making it peoples of this land. year. And that doesn’t include all the particularly diffi cult to meet your renewals of these great CDs and DVDs! out-of-town guests’ requests to • NMAI, established by an act Visit the Scientifi c Library to view the visit the Smithsonian during their of Congress in 1989, is the fi rst whole collection and make your picks weekend visits to Frederick!). national museum dedicated to the for a cozy, free weekend entertainment. preservation, study, and exhibition You bring the popcorn! Ø

The NCI-Frederick Poster 24 September 2006 New Faces at NCI-Frederick

NCI-Frederick Welcomes New Staff Seventy-fi ve people joined our Facility in April, May, and June 2006.

NCI-Frederick welcomes… Sarah Aherne Rieko Ajima Charles Rivers Laboratories Imla Hassen welcomes… Ravi Kalathur Shuhei Kotoshiba Kenneth Kline Hehua Liu Erika Marion George Lountos Crystal Osborne Ø Frederica Polato Ø

SAIC-Frederick, Inc., welcomes… Alexander Adelsberger William Jacob Sarah Aherne Ryan Jenkins Anuli Ajene Cassandra Jennings Mia Alilin Patti Labbe Naing Aung Hongchuan Li Babak Behbahani Ling Li Sarah Bowie Fung Lian Christina Burks Ping Liao Victor Carr III Van Ling Kristi Pearson Octavio Quinones Juan Ceballos Sylvan Mcdowell Onekoko Chaw Andrew Mckay Anthony Clark Olivia Mejia Timothy Cole Danielle Miller Heather Cooley Kevin Miller Stephen Darnell Jacob Minang Tiziano Dipaolo Jacinto Noriega Anne Dudley Carmen Ortiz Scott Emerick Kristi Pearson Mirtis Fonseca Thomas Pfi ster Lakeisha Galloway Richard Pilch Melaku Gedil William Ryan Data Management Services Thomas Gollery Michael Schuit welcomes… Colin Gray Tamika Segura Abrar Hashmi Cindy Selby Christopher DeVaughn. Wei Shao Octavio Quinones Melanie Simpson Jean Roayaei Ø Christina Sinchak Christine Spalding Frederica Polato Reyna Strohecker Stephen Darnell Tony Tejera Rasmi Thomas Ismahan Ugas Elizabeth Webb Preston Weedon Xinyu Wen Sarah Wilcoxon Wenze Xi Yiping Zhang Ø

The NCI-Frederick Poster 25 September 2006 Special Events

Summer Student Poster Day

Take Your Child To Work Day

The NCI-Frederick Poster 26 September 2006 Write When You Get Work

“Write When You Get Science and Humanities Symposium to “a wonderful husband,” and has Work” competition, and a second place in two children, ages 4 and 6—all in a Editor’s Note: This is the fi rst in a series of the 1995 Intercollegiate Chemistry little more than 10 years after leaving articles on former interns from the Werner H. Conference at Muhlenberg College. NCI-Frederick. Kirsten Student Intern Program (SIP). We’ve As a graduate student at the University entitled it “Write When You Get Work” to echo of Pennsylvania School of Dental Infl uence of the NCI-Frederick a the commonly heard “send-off” for young Medicine, her research with Dr. Hyun Internship adults leaving home. Duck Nah-Cederquist in craniofacial Dr. Kim appreciates the impact Yooson Eugina Kim, DDS bone development earned third place the student internship had on her in the Caulk/Dentsply Research life decisions. She explained, “My Dr. Yooson Eugina Kim was a Competition at the 1996 meeting of experience as an intern opened Werner H. Kirsten student intern in the International doors for me and the early years of the program, which Association of gave me invaluable began in 1989. From 1990 to 1991, Dental Research. experience. At she was an intern in the Laboratory Following the University of Comparative Carcinogenesis under dental school, she of Pennsylvania, the mentorship of Dr. Lucy Anderson, completed a two- my research work and she continued working in the lab year residency helped me establish during the summers throughout her in oral medicine professional respect college years. Dr. Kim believes her at the Hospital from faculty and lifelong friendship with Dr. Anderson of the University gave me career represents her most signifi cant of Pennsylvania, opportunities as achievement while working here. She where, she said, soon as I walked also was proud to earn a number of she “switched on campus.” In awards while at NCI-Frederick: in gears” and addition, she said the addition to winning grand prizes at moved into internship prepared local and state science fairs, she was a clinical service. Working with Dr. her for “writing scholarly papers, semi-fi nalist in the 1990 Westinghouse Michael Glick, she studied the effi cacy presenting papers and posters, and Science Talent Search, Washington, of different treatment modules, the critical thinking. I was able to gain DC; third place winner in the 1990 incidence and prevalence of HIV/AIDS- the respect of my colleagues and Intel International Science and related oral conditions and treatments, instructors for the skilled, careful Engineering Fair, Orlando, FL; and and a clinical trial of a new treatment analysis of each situation.” vice-president of the Maryland Junior for temporomandibular disorder. Scientists, 1990–91. Advice for Interns: “Listen” After graduating from Frederick Time to Be Her Own Boss One word sums up Dr. Kim’s advice High School in 1991, she continued After her residency, she began for student interns at NCI-Frederick: earning practicing general dentistry, spending Listen. She went on to say, “The awards, two years in associateships before advice you receive is invaluable; do fi rst at deciding it was time to be her own not ignore it. Hear what people tell Franklin and boss. In 2003, she bought a practice you and put it into action. Often the Marshall in Morgantown, PA, and, she said, work can be tedious, repetitive, and College, “within two years, I bought land, built frustrating. Plug on through and keep Lancaster, a new offi ce building, and am now improving yourself as your mentor PA, continuing to expand with the help of guides. Utilize every opportunity, where she an energetic and loyal staff.” [and] if you don’t see any you like, majored in Today she is most proud of her then make a path for yourself.” chemistry. recent accomplishments, and who Clearly, Dr. Kim has not been afraid Space does can blame her: she earned a degree in to make her own path. Please join not permit dental medicine, owns and operates a the staff at The Poster in wishing her listing them all, but highlights include successful dental practice, is married continued success. Ø fi rst place at the 1992 National Junior

The NCI-Frederick Poster 27 September 2006 Data Management Services (DMS)

Three Receive Longevity Visit the C&SS Web site at http://css. Site-Licensed Software Awards ncifcrf.gov or call 301-846-1060 for Available from the Helpdesk! information about custom development Three DMS employees recently services available from C&SS. C&SS, in conjunction with NCI, has received Longevity awards for their worked to secure site licenses for many years of dedicated service. Combined, Technology Advocacy and of the programs in broad use at NCI- they have provided their expertise Consultation Frederick. To view the growing list of to NCI-Frederick for 45 years! The As NCI-Frederick’s information software available from the Helpdesk, recipients include Ed Green, 30 years; technology experts, C&SS continually visit the C&SS Web site at: http://css. Sophia Bedard, 10 years; and Tim explores and evaluates new ncifcrf.gov/helpdesk/software.asp Siford, 5 years. Ø technologies that could benefi t the or contact the Computer Services Data Management user community and further NCI- Helpdesk to borrow the software or Frederick’s mission. C&SS staff would request installation assistance. Ø Services: Computers and be happy to meet with you to discuss Statistical Support your specifi c technology needs. Although perhaps most widely Contacting C&SS known for our Microcomputer Support Computer Software Computer Services Helpdesk and Web Development services, C&SS Training Web: http://css.ncifcrf.gov/helpdesk also offers many other services to the The summer 2006 training session E-mail: [email protected] NCI-Frederick community. In this is now closed. Watch for postings Phone: 301-846-5115 issue of The Poster we highlight some and e-mail notifi cations for the of these other services. fall/winter schedule. Hours of Operation: Please see the Computer Software 8:00 a.m.–5:00 p.m., Statistical Consultation Training Web site at http://css.ncifcrf. Monday through Friday The Statistical Consultation gov/training for more information or group provides a wide array of to register for classes. NCI-Frederick Webmasters mathematical and statistical consulting Phone: 301-846-6700 services to the NCI-Frederick Computer Services Helpdesk E-mail: [email protected] scientifi c community. The director The Computer Services Helpdesk [email protected] and consulting statisticians work provides the NCI-Frederick in collaboration with principal community with a single point of Other Inquiries investigators through all facets of the contact for computer assistance, Phone: 301-846-1060 Ø scientifi c process: from development information, service, and support. The and formulation of research and Helpdesk is staffed from 8:00 a.m. to statistical hypotheses through design 5:00 p.m., Monday through Friday, of experiments and statistical analyses, excluding NCI-Frederick holidays. preparation of technical reports and Requests for service can also be modern graphics, to preparation of placed via the C&SS Web site (http:// formal scientifi c documents and css.ncifcrf.gov/helpdesk) at any time. publications in peer-reviewed journals. Custom Software Development Our team of analysts and developers employs the most modern methods and tools to create custom software solutions to meet the unique needs and requirements of NCI-Frederick. Our staff can assist you with both administrative and scientifi c programming needs, as well as Web design and development services.

The NCI-Frederick Poster 28 September 2006 SAIC-Frederick, Inc.

Frank Blanchard: New Public Frederick’s partnerships with extramural FME Management Awards Affairs Director investigators, as well as the local community. “Both benefi t greatly from The Facilities Maintenance and the work we do here,” he explained. Engineering (FME) Directorate A graduate of the University of believes that all injuries are Florida, Mr. Blanchard began his preventable. Each calendar year FME career as a news reporter in Alabama. recognizes safety accomplishments He moved to the Associated Press and which fall into one of the following covered the U.S. Centers for Disease categories: (1) Zero Recordable Control at the start of the AIDS crisis. Injuries—Shop operations that have He later joined the University of executed their daily activities without Michigan as a senior science writer, incurring an OSHA-recordable covering basic sciences, engineering, injury for the calendar year. (2) Most and medicine. After a stint at the Improved—The shop operation that Howard Hughes Medical Institute, has achieved signifi cant improvement he worked for the past 12 years as in reducing OSHA-recordable Frank Blanchard, communications director for the injuries compared to the previous Director, Public Affairs Whitaker Foundation. year. (3) Safety Leadership—A group Among the more recent “new hires” at SAIC-Frederick, Inc., is Frank Blanchard, Director of Public Affairs. David Bufter, Director of Contracts and Administration, said, “Frank brings a wide range of communication and public affairs experience to NCI- Frederick. His initial focus will be on developing key messages about NCI-Frederick’s mission and value as a Federally Funded Research and Development Center. He will work Representatives of several of the Facilities Maintenance and Engineering shops with NCI communications staff gathered for a photo recently, showing the plaques their groups were awarded. Left to develop an integrated outreach to right: Gene Gruden, Telecommunications Shop, for Zero Recordable Injuries; Walt program that will raise the visibility Smith, Millwright Shop, for Zero Recordable Injuries; Bill Lonergan, Director, FME; of NCI-Frederick among important Tim Lenhart, FME Safety Committee Chairperson; Tom Zimmerman, Pipe Shop, Zero constituencies and respond to inquiries Recordable Injuries; Jay Walsh, Electric Shop, Zero Recordable Injuries; Dave Davis, regarding NCI-Frederick. These Support Shop, Most Improved and Safety Leadership. include intramural and extramural researchers, residents and leaders of With such an extensive journalistic or individual award for educating, the Frederick community, and key background, it’s perhaps no surprise promoting and otherwise leading policy-makers.” that Mr. Blanchard has contributed a safety in the workplace by example. Ø As public affairs director, Mr. chapter to a book: “Communicating Blanchard will focus on making Science from Institutions: Nonprofi ts,” Changes in Management both internal and external audiences A Field Guide for Science Writers, Dante Tedaldi recently was named aware of the breadth and depth of the the Offi cial Guide of the National Acting Manager of Operations, research done at NCI-Frederick to Association of Science Writers, Second while continuing as Deputy Director support the health of the nation. He’ll Edition (eds. Deborah Blum, Mary of FME. His new responsibilities do this through newspaper articles Knudson, and Robin Marantz Henig; include overseeing project managers, and press releases, as well as speeches Oxford University Press, 2005). He also Contracting Offi cer’s technical to local organizations and national writes occasional articles for various representatives, and building congressional offi ces. Mr. Blanchard magazines and newspapers, and creates coordinators. In addition, some other said that he wants to build on NCI- 15-grid crossword puzzles for friends. Ø continued on page 31 The NCI-Frederick Poster 29 September 2006 Wilson Information Services Corporation (WISCO)

Employee Recognition to track the number of customers we have also helps us to predict staffi ng Scientifi c Library Two WISCO employees celebrated levels, set convenient service hours, Orientation Sessions milestone anniversaries recently. and forecast facility improvements. Pamela Noble has been with the Finally, maintaining a safe and October 11, November 8, and Scientifi c Library for 15 years, while secure environment is made easier by December 13 at 2:30 p.m. knowing who is in the building. Although we have always kept the For details about upcoming fall sign-in logs confi dential, removing training and R.E.W.A.R.D.S. each signature sheet as it was programming, link to http:// completed, there has always been www-library.ncifcrf.gov:8086/ some concern that anyone who entered newlab2005 and http://www- could see the names of others who had library.ncifcrf.gov/rewards.aspx. Ø signed in earlier on the same sheet. Now there is no need to worry. We Hungyune recently installed an electronic sign- Chao recently in system with a touch-screen which completed her goes blank after you have entered your Upcoming Events fi fth year with name and company affi liation. the Library. Ø We have implemented new Current Topics in Genome procedures to protect your privacy in Analysis: another way. Now whenever you use our public computer workstations, just The National Human Genome scan your library barcode, activating Research Institute (NHGRI) is News from the Center for the color copier and printers. This data sponsoring a mini-course covering Health Information (CHI) is automatically tracked by computer. contemporary areas in genomics Stop by the Scientifi c Library to look Printing and copying are free to NCI- and bioinformatics. Lectures are at some of the items recently added to Frederick employees, and available being videoconferenced from NIH the CHI collection. It’s never too late for a nominal fee to the public. Don’t to the NCI-Frederick Conference to improve your physical fi tness, and have a barcode? Just register at the Center, Tuesdays, September 5 we have 10 exercise DVDs to help you Circulation Desk. through December 12, 2006, from get started. They cover Pilates, yoga, Library circulation and registration 10:00 to 11:30 a.m. tai chi, walking programs, and even the records that contain personal names “Biggest Loser” workout sessions. Why are protected by most state laws, Principles of Clinical not take a walk at lunch time and see the including Maryland’s. Librarians have Pharmacology: Sponsored by collection? You’ll be glad you did! Ø an ethical responsibility to protect the the Clinical Center at NIH, privacy of all library users, so that this program provides “a series Protecting the Privacy of everyone has the right to open inquiry of lectures that cover much Library Users without having the subject content of the scientifi c basis of the If you have ever visited the Scientifi c or frequency of inquiry identifi able bridging discipline of clinical Library, you know that we ask you by others. Our data on library visits pharmacology,” according to the to sign in, for several good reasons: and copying/printing is tabulated for Clinical Center’s Web site, http:// Keeping a record of the people who statistical purposes and then destroyed. clinicalcenter.nih.gov/researchers/ enter helps us identify anyone who To learn more about intellectual training/principles.shtml. Lectures has received a telephone call through freedom, please visit the American are being videoconferenced our offi ces or who is waiting to meet Library Association’s Web site at from NIH to the NCI-Frederick someone. Because we are open to the http://www.ala.org/Template.cfm? Conference Center Thursdays, public during weekdays, asking visitors Section=otherpolicies&Template=/ September 7 through April 26, to sign in provides us with valuable ContentManagement/ContentDisplay. 2007, from 6:30 to 7:45 p.m. statistics to show which agencies use cfm&ContentID=13087. Ø Registration is required. Ø the facility and how often. Being able

The NCI-Frederick Poster 30 September 2006 Poster Puzzler Winner

The June Poster Puzzler winner: Wade Schirmer, Contracting Offi cer’s Technical Representative, Facilities Maintenance and Engineering pictured here with Paul Miller, Executive Editor of The Poster, in front of Building 459.Ø

continued from page 29 Interview/Profi le” editorial category. managers’ responsibilities have “Postgraduate Researcher: Biotech been realigned to better use the new Student Has Aimed for Science Career computerized maintenance system that Since Age 4” (The Poster, September Data Management Services is installing. 2005), by Maritta Perry Grau, senior These managers include Tim Lenhart, technical editor, won a Bronze Award, Debbie Dobbe, and Len Wrona. SPGM Wins Silver and Bronze also in the “Best Interview/Profi le” Mr. William Lonergan, Director in Magnum Opus Awards editorial category. of FME, stated in a memorandum Scientifi c Publications, Graphics “This is our best showing to date; that Mr. Tedaldi “is well known & Management (SPGM), Research in previous competitions, we’ve won for his energy, enthusiasm, and Technology Program, was recently Honorable Mentions and one Bronze dedication, and brings more than 15 notifi ed that two articles have Award. This is our fi rst Silver,” said years of project management and received silver and bronze awards Ken Michaels, SPGM manager. engineering experience to his new from Magnum Opus, an annual The Magnum Opus competition role in FME. His strong management competition sponsored by Publications received nearly 700 entries in 53 skills, Six Sigma training, drive for Management and held in conjunction categories, “which honor excellence excellence, and unique perspective with the well-known Missouri School of editorial, design and strategy of will add considerable value to FME’s of Journalism. custom publications,” according continuing efforts to provide world- “SIP Student Winners at International to Publications Management. class services to the NCI-Frederick Science Fair” (The Poster, June 2005) For more information on the campus.” Ø by Nancy Parrish, senior technical awards, visit mcmurry.com/award/ editor, won a Silver Award in the “Best magnumopusawards.asp. Ø

The NCI-Frederick Poster 31 September 2006

The Poster Staff Employment Opportunities Executive Editor Paul Miller Associate Editor Please contact the individual contractor’s human resources Ken Michaels representatives or go to the contractor’s Web site for Managing Editor up-to-date, detailed information about jobs or research Maritta Grau and training opportunities and requirements. Co-Editor Nancy Parrish Production Editor Charles River Laboratories Kathy Green http://www.criver.com Lead Designer Tammy Schroyer Photography Editors Data Management Services Jonathan Summers Marti Welch http://css.ncifcrf.gov/about/dms.htm Contributing Editors National Cancer Institute at Frederick Administrative Resource Center http://www.training.nih.gov/postdoctoral Debbie Dixon Judi Carter Tanya Sappington SAIC-Frederick, Inc. Charles River Laboratories Cliff Hubbard http://saic.ncifcrf.gov Community Outreach www.saic.com Barbara Birnman Julie Hartman Data Management Services Wilson Information Services Corporation Stephanie Sheppard http://www-library.ncifcrf.gov Facilities Maintenance and Engineering Deborah Dobbe Environment, Health, and Safety Program Look for the Following Events Around Campus: Alberta Peugeot Frederick Employee Diversity Team Poster Puzzler—Entry Deadline: October 27, 2006 Scott Keimig Fitness Challenge Learning Lunches—second Thursday of each month. Fisher BioServices Check the Web site for details: http://saic.ncifcrf.gov/fi tnesschallenge/ Kathleen Groover Patricia Hindes Farmers’ Market—Every Tuesday through October 31, 11:30 a.m.–1:30 p.m. SAIC-Frederick, Inc. (or sellout) Dave Bufter Science Today Paul Nisson Wilson Information Services Corporation Sue Wilson Robin Meckley Reminder: When you have a change in staff, such as new staff, a promotion, retirement, loss of staff, be sure to change the information on the NCI-Frederick database. You can do this online by Published four times a year by Scientifi c logging on to http://web.ncifcrf.gov/campus/phonebook/, or by contacting your human resources Publications, Graphics & Media for the National Cancer Institute at Frederick, representative. For more information, you may refer to the inside front cover of the NCI-Frederick Frederick, MD 21702. Telephone & Services Directory. http://web.ncifcrf.gov/ThePoster Comments or suggestions for The Poster may be directed to http://web.ncifcrf.gov/ThePoster

The National Cancer Institute at Frederick

Frederick, MD 21702-1201