Sealing Solutions

Product Overview About us Manufacturing & Quality Standards

About us 4titude® Ltd is a market leader in the design and manufacture of consumables and bench top instrumentation for a range of fields within the life sciences industry, from Research to Molecular Diagnostics. Since our inception in 2005, 4titude® has grown and developed internationally, with facilities in Germany, France, USA, Hungary, and our UK-based head office and manufacturing plant located in the Surrey Hills, south of London. The state-of-the-art manufacturing facility boasts 25,000 square feet of floor space, producing consumables for a wide range of life science and medical applications, including research into combatting cancer and infectious diseases, drug development, molecular diagnostics, and forensics. Current off-the-shelf products include PCR consumables, sealing materials and heat sealing instrumentation, microplates for cell screening and storage, and solutions for sample tracking, including barcodes and 2D data matrix codes on products. In addition to these products, we have the capability to offer completely tailored solutions, from custom design of bespoke products, right through to tool making & manufacturing of the parts. 4titude® can offer you a complete solution from concept to completion.

4titude® manufacturing & quality standards 4titude® are ISO 9001:2008 & ISO 13485:2012 certified to manufacture and supply consumables for the life sciences sector. Our management systems comply with the requirements to produce medical devices which we sell to diagnostics companies of all sizes including multinational corporations. We also provide complete custom design solutions from prototyping to tool design and contract manufacturing.

Manufacturing standard • ISO 9001:2008 & ISO 13485:2012 certified • Process validation & mapping • Fully document controlled manufacturing processes • Statistical analysis of production processes • Continuous improvement programs • Injection moulding in ISO class 7 cleanrooms • Virgin, medical grade polymers

Quality standard 4titude® performs visual, physical and biological tests to ensure the integrity of our consumables and that they are contamination free at all times.

• Consumables are certified free from human genomic DNA, • PCR inhibition tests are performed on polymers used nucleases and pyrogens • Leak tests are performed on every well of every PCR plate • Skirted microplates and PCR plates meet the SBS standard • White-well plates are checked for background fluorescence footprint

2 | Contact us: [email protected] For more information see www.4ti.co.uk Sealing Solutions

Contents

About us 2 Sealing Solutions 3 Heat Sealing Introduction 4 Heat Sealing Consumables 5 Heat Sealing Consumables Comparison Table 10 Random Access Heat Sealing 12 FrameSeal™ & Thermal Test Film 13 a4S Automatic Roll Heat Sealer 14 4s3™ Semi-Automatic Sheet Heat Sealer 16 Adhesive Sealing Consumables & Accessories 18 Adhesive Sealing Consumables Comparison Table 24 Caps, Lids & Mats 26

Sealing Solutions 4titude® offers the widest range of plate sealing solutions available on the market. You can choose between sealing with strip caps, mats, lids, adhesive seals in strip or plate format, and heat seals in flexible formats up to plate size. The choice of an optimised sealing solution is particularly important for (q)PCR because thermal cycling can be associated with evaporation of reaction reagents.

Heat Sealing & Adhesive Sealing Dependant on your application requirements we offer a wide selection of materials to choose from within both our adhesive seal and heat seal ranges, You have the option to choose your seal based on a wide variety of properties offered, including peelability, pierceability, gas permeability, optical clarity, temperature stability and solvent resistance. The best sealing results can be obtained by using flat rigid plates like FrameStar® plates and heat seals, using reliable, high quality sealing instruments for seal application, like the 4s3™ Semi-Automated Sheet Heat Sealer or the a4S Automated Roll Heat Sealer. Alternatively, popular adhesive seals need to be applied well using a seal roller or seal applicator. 4titude® seals are produced and processed under strictly controlled environmental conditions and according to our ISO standard manufacturing. All of our seals are DNase, RNase, human genomic DNA, dust, endotoxin/pyrogen free. Dimensional and functional tests are performed on all production lots. If your seal of choice is not offered sterile as a stock product, then please contact us; we can offer custom sterilisation of any seals if required.

Caps, Lids & Mats As an alternative to sealing films, 4titude® offers multiple types of cap strips for sealing of both plates and tubes - domed and flat, strips of 8, strips of 12, and our new optically superior CrystalStrips™. A variety of rigid polystyrene lids are available for PCR plates and microplates, as well as silicone sealing mats for our storage plate range. We offer lids that are compatible with our FrameStar® PCR plate range, and all assay plate ranges including the Vision Plate™ range and UltraVision™ Plates. The silicone cap mats are for use with our storage plates, and come in a variety of formats depending on the plate.

For more information see www.4ti.co.uk/seal Contact us: [email protected] | 3 Heat Sealing Consumables & Instrumentation

Heat Sealing Consumables & Instrumentation Heat sealing is the gold standard method of plate and tube sealing. It prevents sample loss and maximises sample security, by ensuring a complete seal and preventing evaporation, leakage and contamination. The sealing performance of heat seals is superior to all other methods including cap, mat and adhesive sealing. The variability of sealing integrity seen when using adhesive seals, caps or mats is reduced. The optimised sealing performance of a heat seal allows the use of smaller reagent volumes, leading to reagent cost savings and thus making heat sealing the most cost efficient sealing method for a wide range of applications. Heat seals are available as sheets, for manual or semi-automatic heat sealers, as well as in different roll formats for automated sealers. 4titude® sealing consumables are compatible with a wide range of heat sealers, please refer to the instrument compatibility table on page 10. Depending on throughput, we recommend our 4s3™ Semi-Automatic Sheet Heat Sealer (page 16) or a4S Automated Roll Heat Sealer (page 14) for applying your heat seal. In addition to instruments and consumables we also offer a Thermal Test Film for the optimisation and troubleshooting of heat sealing applications and a unique frame mounted heat sealing sheet for low throughput automation (page 13).

Economic efficiency Investing into heat sealing solutions leads to a per plate reduction of sealing costs for all applications including storage, PCR and qPCR as shown in the table below for (q)PCR. The cost is further reduced by changing to the use of rolls seals rather than sheet seals. Application PCR Application qPCR Sealing Solution Product Costs/plate Product Costs/plate Caps 4ti-0751, Strips of 8 Flat Caps £2.90 4ti-0755, CrystalStrip™ £3.04 Adhesive seal, sheet format 4ti-0500, PCR Seal £0.85 4ti-0560, qPCR Seal £1.44 Heat seal, sheet format 4ti-0541, Clear Heat Seal £0.49 4ti-0541, Clear Heat Seal £0.49 Heat seal, roll format 4ti-0540, Clear Heat Seal £0.13 4ti-0540, Clear Heat Seal £0.13 Table 1: Exemplary per plate sealing costs (reference: UK List price 2017)

Save your time The application of heat seals is also easier and faster than when using caps or adhesive seals. Typical sealing times of a semi- automatic heat sealer, such as the 4titude® 4s3™ Semi-Automatic Sheet Heat Sealer, are around 2.5 seconds. A fully automatic roll heat sealer, such as the 4titude® a4S Automatic Roll Heat Sealer, allows for sealing cycle times of less than 15 seconds.

Set your standard Reproducible sealing quality can be guaranteed by standardising the sealing parameters: time, temperature and pressure. 4titude® offers the widest choice of heat seal materials available, with sheet formats for manual or semi-automatic heat sealers and roll formats for automated heat sealers.

Choose your application Depending on the material of the plate (PP, PE, PS, COC, PC), the presence of solvents like DMSO in your sample and the storage or application temperatures required, we can offer a wide range of seals covering 100� DMSO storage and sealing integrity temperatures between -200˚C to +120˚C. Custom sealing materials or sizes can be produced, please contact us at [email protected].

Highest quality standards Our seals are produced according to our ISO standard manufacturing. All of our seals are DNase, RNase, human genomic DNA, dust, endotoxin and pyrogen free. If your seal of choice is not offered sterile as a stock product then please contact us as we can offer custom sterilisation of any seals if required.

4 | Contact us: [email protected] For more information see www.4ti.co.uk/seal Heat Sealing | Consumables 5 | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 50 50 100 100 100 100 Case Case Case Case / / / / 2 2 2 4 seed storage seed 1 1 1 1 1 3 / [email protected] SHS)

- 1 / Sheets or Rolls Sheets Sheets or Rolls Sheets Sheets or Rolls Sheets or Rolls Sheets Contact us: 4ti 2 2 1 1 Stacking Heat Sealers (LHS Sealers Heat Stacking

/ Autoclavable Peelable Peelable peelable, difficult to pierce, suitable for qPCR, for qPCR, suitable pierce, to difficult peelable, KBioscience FlexiSeal and Cube FlexiSeal KBioscience

- / (250 m x 78 mm, approx. 2,100 seals) (250 m x 78 mm, approx. 3,140 seals) (250 m x 115 mm, approx. (125 mm x 78 mm) (610 m x 78 mm, approx. 5,000 seals) (610 m x 78 mm, approx. (5 m x 78 mm) 6,250 seals) (500 m x 115 mm, approx. (5 m x 115 mm) (125 mm x 78 mm) (500 m x 78 mm, approx. 4,200 seals) (500 m x 78 mm, approx. (5 m x 78 mm) 4,400 seals) (350 m x 115 mm, approx. (5 m x 115 mm) (125 mm x 78 mm) (5 m x 78 mm) (5 m x 115 mm) (122 m x 78 mm, approx. 1,000 seals) (122 m x 78 mm, approx. (80 m x 78 mm, approx. 640 seals) (80 m x 78 mm, approx. 4,200 seals) (500 m x 78 mm, approx. 4,400 seals) (350 m x 115 mm, approx. 3,800 seals) (450 m x 78 mm, approx. (125 mm x 78 mm) (5 m x 78 mm) C with C with ˚ ˚ 110 110 Once autoclaved, autoclaved, Once * recommended not PCR for * pressurised lid heated * pressurised lid heated * * * C C C C C C ˚ ˚ ˚ ˚ ˚ ˚ 80 80 80 Details Details Details Details Roll Roll Sheets Roll roll Sample Roll roll Sample Sheets Roll roll Sample Roll roll Sample Sheets Sample roll Sample roll Sample Roll frames stackable Mark 2 FrameSeal™ Seal Heat Weld Clear Roll Roll Roll frames stackable FrameSeal™ Seal Heat Clear Roll Sheets Sample roll Sample - - - 110 80 80 Wasp™ KBiosystems

/ S with REMP Landscape Compatible / 4

S S 122 FS 80 REMP REMP FS SLIT SLIT SLIT / / / / / / / / / / / 0549 0549 0548 0548 05481 0573 0573 0573S 0574 0574S 0575 0575 0540 0540 0540 0540S 0542 0542 0542S 0541 0541 0540 0540 0541 ------4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti optical applications and storage applications optical Code Code Clear Weld Heat Seal Mark 2 Seal Heat Weld Clear film, non sealing heat clear Optically Plus Seal Heat Clear mill for bead suitable peelable, clear, film, optically sealing heat strength High tensile applications Code Clear Heat Seal Heat Clear applications for qPCR and optical suitable clear, film, optically sealing heat Peelable Code Seal Heat Clear Gas Permeable studies insect for suitable gas transfer, for with 3 mm slits Seal, Heat Clear 4ti 4ti 4ti 4ti 4ti Seal Integrity Integrity Seal Range Temperature Integrity Seal Range Temperature Seal Integrity Integrity Seal Range Temperature Thermo Fisher ALPS 300™ and ALPS 3000™ 300™ and ALPS Fisher ALPS Thermo

/ seal / Compatible with REMP Portrait Heat Sealer (PHS) · (PHS) Sealer Heat with REMP Portrait Compatible 3 · ® seed storage seed / qPCR, even without the use of a of without the use even qPCR, Sealer Heat Roll a4S Automatic ® / Forming a peelable seal to PP, PS and COC plates plates and COC PS PP, to a peelable seal Forming microplates of sealing for strength tensile Excellent or other seeds of or disruption during homogenisation mill applications such as bead material Permanent seal Permanent PCR for Suitable Optically clear, peelable clear, Optically and detection, imaging, fluorescent for Suitable assays colorimetric imaging techniques qPCR and other for Recommended including crystallisation with 3 mm Seal Heat Clear as Gas Permeable Available studies insect for suitable slits, pressurised heated lid heated pressurised uses high temperature and very low for Suitable Compatible with 4titude Compatible 11) PlateLoc (Velocity with Agilent Compatible For more information see www.4ti.co.uk 2 Features • • Clear Heat Seal Plus Seal Heat Clear Features • • Clear Weld Heat Seal Mark 2 Seal Heat Weld Clear Features • • • • 1 Clear Heat Seal Heat Clear • 6 Heat Sealing | Consumables | Contact us: 4ti us: Contact • Peel Heat Seal • • • Features • • • • • • Features Universal Peel Heat Seal • • Features 2 1 Clear Heat Seal 3730 • Compatible with Agilent (Velocity 11)PlateLoc Compatible with 4titude temperature uses, suchasPCR Suitable for low temperature sample storage andhigh Suitable for use withABI Easily pierceable withautosampler needles Forming apermanent seal to PP, PE,PS andCOC plates temperature uses, suchasPCR Suitable for low temperature sample storage andhigh sealing of different plates withouttheneedfor roll changes Wide material compatibility allows for highthroughput directly ontop of apreviously pierced seal Resealable by applyinganother Universal Peel Heat Seal Pierceable withneedle, butnot standard pipette tips Peelable Compatible with PP, PE,PS, COC andPCplates removed directly from Peelable from PPplates, even whenaplate hasbeen Laminate seal compatible withPPandCOC plates colorimetric assays Suitable for imaging,fluorescent detection, and the needfor theuse of expensive septa mats - [email protected] ® a4SAutomatic Roll Heat Sealer - 80°C storage ® 3730sequencers removing ® · 3 Compatible with REMPPortrait Heat Sealer (PHS) · /

Thermo FisherALPS 300™andALPS 3000™ Temperature Range Seal Integrity Temperature Range Seal Integrity Temperature Range Seal Integrity Code 3730, suitable for PCR, qPCRand optical applications Peel Heat Seal Thin polyester heat sealing film, easily pierceable withautosampler needles Clear Heat Seal 3730 Code uses andPCR Peelable heat sealing foil, suitable for low temperature storage, hightemperature Universal Peel Heat Seal Code uses andPCR Peelable heat sealing foil, suitable for low temperature storage, hightemperature 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti ------05231 0524S 0524 0523S 0523 0521 0522S 0522 0520S 0520 0520 0581 0582S 0582 0580S 0580 0580 / / 122 122

4 Compatible with REMPLandscape /

KBiosystems Wasp™ 80 - - - Details Roll Roll Sheets Sample roll Roll Sample roll Roll Sheets Sample roll Roll Sample roll Roll Sheets (125mmx78mm) onperforated roll Sample roll Roll Sample roll Roll Details Details 90 90 80 20 80 ˚ ˚ ˚ ˚ ˚ ˚ C C C C C C * * * * * heated lid pressurised heated lid pressurised * heated lid pressurised 110 110 110 ˚ ˚ ˚ C with C with C with C with C with (122 mx78mm,approx. 1,000seals) (122 mx78mm,approx. 1,000seals) (125 mmx78mm) (5 mx115mm) (500 mx115mm,approx. 6,250seals) (5 mx78mm) (610 mx78mm,approx. 5,000seals) (125 mmx78mm) (5 mx115mm) (500 mx115mm,approx. 6,250seals) (5 mx78mm) (610 mx78mm,approx. 5,000seals) (5 mx115mm) (500 mx115mm,approx. 6,250seals) (5 mx78mm) (610 mx78mm,approx. 5,000seals) / For more information see www.4ti.co.uk see information more For

KBioscience FlexiSeal andCube Autoclavable Autoclavable Pierceable /

Stacking Heat Sealers (LHS 1 1 1 2 2 2 Peelable Peelable Sheets orRolls Sheets orRolls Sheets orRolls /

SHS) 1 1 1 1 1 2 2 2 / ABI 1,000 / / / ® Case Case Case

/ 100 100 seal 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Heat Sealing | Consumables 7 | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 100 100 100 100 Case Case Case / / / / / 2 3 2 2 3 1 1 3 1 1 3 [email protected] 1 SHS)

- / Sheets or Rolls Sheets Sheets or Rolls Sheets Sheets or Rolls Sheets Contact us: 4ti 2 2 2 1 1 1 Pierceable Stacking Heat Sealers (LHS Sealers Heat Stacking

/ , with printed grid reference , with printed KBioscience FlexiSeal and Cube FlexiSeal KBioscience

/ as above (500 m x 115 mm, approx. 6,200 seals) (500 m x 115 mm, approx. (5 m x 115 mm) (125 mm x 78 mm) (610 m x 78 mm, approx. 5,000 seals) (610 m x 78 mm, approx. (5 m x 78 mm) 6,200 seals) (500 m x 115 mm, approx. (5 m x 115 mm) (125 mm x 78 mm) 5,000 seals) (610 m x 78 mm, approx. (5 m x 78 mm) (500 m x 78 mm, approx. 4,200 seals) (500 m x 78 mm, approx. (5 m x 78 mm) 6,200 seals) (500 m x 115 mm, approx. (5 m x 115 mm) (122 m x 78 mm, approx. 1,000 seals) (122 m x 78 mm, approx. 5,000 seals) (610 m x 78 mm, approx. 6,200 seals) (500 m x 115 mm, approx. (100 m x 78 mm, approx. 800 seals) (100 m x 78 mm, approx. 4,200 seals) (500 m x 78 mm, approx. 6,200 seals) (500 m x 115 mm, approx. Peelable Pierceable Autoclavable C C C C C C ˚ ˚ ˚ ˚ ˚ ˚ 20 80 20 Details Details Details Roll roll Sample Sheets Roll roll Sample Roll roll Sample Sheets Roll roll Sample Roll roll Sample Roll roll Sample roll on perforated mm) 78 x mm (125 Sheets Roll Roll Roll Sheets Roll Roll Roll 40 - - - 120 120 Wasp™ KBiosystems

compound storage compound / / with REMP Landscape Compatible 4

122 REMP REMP GR 100 REMP REMP / / / / / / / shipping / 0539 0539S 05381 0530 0530 0530 0530S 0532 0532 0532S 0531 0531 0538 0538S 0585 0585 0585 0585S 0586 0586 0586S 0587 ------Code Code 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti Pierceable heat sealing foil, high solvent resistance, resealable, suitable for PCR for suitable resealable, resistance, high solvent foil, sealing heat Pierceable storage Strong Seal Heat Pierce PCR for suitable pierceable, plates, COC peelable from foil, sealing heat Strong shipping sample DMSO Resistant Peel Heat Seal Heat Peel Resistant DMSO temperature and room low for suitable peelable, foil, sealing heat resistant Solvent storage compound Code Seal Heat Pierce Also available as a Random Access Seal, 96 individual seals in sheet or roll format, format, or roll in sheet 96 individual seals Seal, Access as a Random available Also page. following see 4ti Seal Integrity Integrity Seal Range Temperature Seal Integrity Integrity Seal Range Temperature Integrity Seal Range Temperature Thermo Fisher ALPS 300™ and ALPS 3000™ 300™ and ALPS Fisher ALPS Thermo

/ seal / Compatible with REMP Portrait Heat Sealer (PHS) · (PHS) Sealer Heat with REMP Portrait Compatible 3 · ® suitable for low temperature temperature low for suitable - Sealer Heat Roll a4S Automatic ® Compatible with PP (gives a weld seal) and COC and COC seal) a weld with PP (gives Compatible plates (peelable seal) a liquid or by tip manually, with a pipette Pierceable handling robot and room shipping, low sample PCR, for Suitable and other with DMSO storage compound temperature solvents organic Compatible with PP and PS plates with PP and PS Compatible PCR for Suitable resistance Good solvent Peelable from PP and COC plates, forms a weld seal to to seal a weld forms plates, PP and COC from Peelable PE plates compound temperature and room low for Suitable solvents and organic in DMSO storage and room temperature compound storage in DMSO and and in DMSO storage compound temperature and room solvents organic reference grid printed with format sheet in also Available Compatible with 4titude Compatible 11) PlateLoc (Velocity with Agilent Compatible For more information see www.4ti.co.uk 2 Features • • • Pierce Heat Seal Strong Seal Heat Pierce Features • • • Pierce Heat Seal Heat Pierce Features • • 1 DMSO Resistant Peel Heat Seal Heat Peel Resistant DMSO • 8 Heat Sealing | Consumables | Contact us: 4ti us: Contact 5 2 1 • • • Features Foil Heat Seal • • • Features 96 INDI™Heat Seal • • • • Features Random Access Pierce Heat Seal Strong Compatible with 4titude Compatible with Agilent (Velocity 11)PlateLoc Compatible with 4titude on top of apreviously pierced seal Resealable by applyinganother Foil Heat Seal directly handling robots Pierceable with apipette tip, manuallyorby liquid Compatible withPPandPS plates, peelable removed to leave 96individuallysealed wells ortubes Following application of thisseal, theframe can be handle sealing sheet 96 individualround seals heldonaconvenient to racked tubes Compatible withmost PP96round well plates and 96 Well Skirted PCRPlate (4ti Best used incombination withourRandom Access applications Recommended for storage, sample shippingandPCR 96 individualfoil seal spots onaremovable backing plates Compatible withPP(weld seal) andCOC (peelableseal) - [email protected] ® ® a4S a4SAutomatic Roll Heat Sealer RA Automatic Random Access Heat Sealer - 0960 ® · 3 Compatible with REMPPortrait Heat Sealer (PHS) · / RA) /

Thermo FisherALPS 300™andALPS 3000™ • • • • • • Temperature Range Seal Integrity Temperature Range Seal Integrity Temperature Range Seal Integrity 4ti Code format 96 foil seal spots for sealing of individualwells for storage orPCR, sheet orroll Random Access Pierce Heat Seal Strong storage Aluminium heat sealing foil, resealable, peelable, pierceable, suitable for compound Foil Heat Seal Code low temperature storage 96 perforated individualsealing caps inafoil frame, peelable, pierceable, suitable for 96 INDI™Heat Seal 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti Code incubations Suitable for low temperature storage andhightemperature Good solvent resistance, includingDMSO using forceps, viatheseal removal tab The individualseals can beremoved asrequired, by handor For details onRandom Access Heat Sealing also see page 12 a4S Roll format: optical windows for exact positioning inthe of the4s3™Semi Sheet format: 4pinholes for exact positioning inspecialadapters ------0539 05381 0536 0537S 0537 0537 0535S 0535 0535 0535 0543 RA / Automatic Random Access Heat Sealer (see page 12) PCR / / / / RA REMP REMP 122 / RA

4 Compatible with REMPLandscape /

KBiosystems Wasp™ -

120 110 120 - 195 Sheets Roll Roll Roll Roll Sheets Sample roll Roll Sample roll Roll Sheets Details Details Details - 20 20 - Automatic Sheet Heat Sealer (see page 16) ˚ ˚ ˚ ˚ ˚ ˚ C C C C C C Autoclavable Pierceable Peelable (127 mmx100mm) PCR plates) (420 mx100mm,approx. 3,200sealed (500 mx115mm,approx. 6,200seals) (610 mx78mm,approx. 5,000seals) (122 mx78mm,approx. 1,000seals) (125 mmx78mm) (5 mx115mm) (500 mx115mm,approx. 6,200seals) (5 mx78mm) (610 mx78mm,approx. 5,000seals) (130 mmx80mm) / For more information see www.4ti.co.uk see information more For

KBioscience FlexiSeal andCube /

5 Stacking Heat Sealers (LHS Pierceable Peelable 1 2 Pierceable Sheets orRolls Sheets orRolls Sheets orRolls /

SHS) 3 1 1 3 2 / / / Case Case Case / 100 100 seal 50 1 1 1 1 1 1 1 1 Heat Sealing | Consumables 9 | 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 10 100 100 100 x Case Case Case / / / 10 2 5 2 1 1 1 1 1 [email protected] 1 SHS)

- / transportation / Sheets or Rolls Sheets Sheets or Rolls Sheets Sheets or Rolls Sheets Pierceable Contact us: 4ti 2 5 2 1 1 1 Peelable Peelable Pierceable Stacking Heat Sealers (LHS Sealers Heat Stacking

/ , sterile KBioscience FlexiSeal and Cube FlexiSeal KBioscience

/ as above (500 m x 115 mm, approx. 6,200 seals) (500 m x 115 mm, approx. (5 m x 115 mm) (125 mm x 78 mm) (610 m x 78 mm, approx. 5,000 seals) (610 m x 78 mm, approx. (5 m x 78 mm) 3,700 seals) (300 m x 115 mm, approx. (5 m x 115 mm) (125 mm x 78 mm) 5,000 seals) (610 m x 78 mm, approx. (5 m x 78 mm) (610 m x 78 mm, approx. 5,000 seals) (610 m x 78 mm, approx. (5 m x 78 mm) 6,200 seals) (500 m x 115 mm, approx. (125 mm x 78 mm) (100 m x 78 mm, approx. 800 seals) (100 m x 78 mm, approx. 1,000 seals) (122 m x 78 mm, approx. (122 m x 78 mm, approx. 1,000 seals) (122 m x 78 mm, approx. (5 m x 115 mm) Peelable Autoclavable Autoclavable C C C C C C ˚ ˚ ˚ ˚ ˚ ˚ 20 20 - Details Details Details Roll roll Sample Sheets Roll roll Sample Roll roll Sample Sheets Roll roll Sample Roll roll Sample Roll Sheets Sheets Roll Roll Roll roll Sample 200 80 - 110 110 - Wasp™ KBiosystems

/ with REMP Landscape Compatible 4

ST 100 122 122 S / / / / / seed and insect storage and insect seed / 0599 0599S 0597 0597 0590 0590 0590S 0592 0592S 0591 0598 0598 0598S 0545 0545 0545S 0546 0546 0547 ------Code 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti Code Thermal Bond Heat Seal Heat Bond Thermal storage long term for suitable peelable, foil, sealing heat duty Heavy Mark 2 Seal Heat Gas Permeable cell for suitable pierceable, peelable, evaporation, limits membrane, sealing Heat culture Polystyrene Foil Heat Seal Heat Foil Polystyrene suitable pierceable, resealable, plates, polystyrene to seals foil, sealing heat Peelable storage compound for Code 4ti Seal Integrity Integrity Seal Range Temperature Seal Integrity Integrity Seal Range Temperature Integrity Seal Range Temperature Thermo Fisher ALPS 300™ and ALPS 3000™ 300™ and ALPS Fisher ALPS Thermo

/ seal / ® Compatible with REMP Portrait Heat Sealer (PHS) · (PHS) Sealer Heat with REMP Portrait Compatible 3 · ® m) enables gas exchange, per 24 gas exchange, enables m) µ , whilst evaporation is reduced to a to is reduced evaporation , whilst 2 Sealer Heat Roll a4S Automatic ® m / compatible with Agilent (Velocity 11) PlateLoc (Velocity with Agilent compatible Compatible with PP, PS and COC plates plates and COC PS with PP, Compatible insect incubations, overnight culture, cell for Suitable storage and seed (<20 size Small pore Compatible with PP plates Compatible a providing for suitable seal foil laminate duty Heavy but peelable seal strong, very as well and transportation storage long term for Suitable storage temperature low very as for Compatible with PP, PS and PC plates and PC PS with PP, Compatible standard than our plates PS to seal a stronger Produces Seal Heat Foil liquid manually or by tip, a pipette with Pierceable handling robots Heat Foil Polystyrene applying another by Resealable seal pierced a previously of on top directly Seal minimum contamination microbial prevents size Small pore contents with the well interfere to without adhesive Surface hours of >20 g of hours NOT Compatible with 4titude Compatible 11) PlateLoc (Velocity with Agilent Compatible For more information see www.4ti.co.uk 2 5 Features • • • Gas Permeable Heat Seal Mark 2 Seal Heat Gas Permeable Features • • • Thermal Bond Heat Seal Heat Bond Thermal Features • • • • 1 Polystyrene Foil Heat Seal Heat Foil Polystyrene • • Heat Sealing Consumables Comparison & Instrument Compatibility Table

Clear Heat Seals Peelable Heat Seals Pierceable Heat Seals Foil Heat Seals Gas Permeable Heat Seals Name Clear Heat Seal Clear Weld Heat Clear Heat Seal Clear Heat Seal Peel Heat Seal Universal Peel DMSO Resistant 96 INDI™ Heat Seal Pierce Heat Seal Pierce Heat Seal Foil Heat Seal Polystyrene Foil Thermal Bond Heat Gas Permeable Heat Gas Permeable Clear Seal Mark 2 3730 Plus Heat Seal Peel Heat Seal Strong Heat Seal Seal Seal Mark 2 Heat Seal Details see page 5 5 6 5 6 6 7 8 7 7 8 9 9 9 5 Specifications Application qPCR PCR, esp. water qPCR and for use Homogenisation Low temperature Low temperature Low/room Compound storage PCR PCR Low temperature Low temperature Low temperature Cell culture Storage e.g. for Short term bath cyclers with ABI 3730 or disruption compound compound temperature Sample shipping Compound storage Compound storage compound storage compound storage transportation & Over night seeds or insects compound qPCR Sequencer of seeds or storage storage compound Short-term room Short-term room storage incubation other material, storage with Low temperature Sample shipping Sample shipping storage Storage & Short term room High temperature storage and high temperature temperature PCR, esp. water Seed and insect e.g. bead mill temperature applications DMSO & other compound storage compound storage bath cyclers disposal of applications organic solvents temperature storage hazardous compound PCR incubations PCR PCR Storage of organic materials storage (<5 days) solvents, acids & PCR alkalines Special Properties Good optical Good optical Good optical High tensile Can be peeled Moderate solvent Can be peeled 96 perforated Easily pierceable Easily pierceable Re-sealable with Re-sealable with Very strong seal Small pore size 3 mm slits across clarity clarity clarity strength directly from resistance directly from individual sealing Resistant to DMSO Resistant to DMSO another Foil Seal another Foil Seal with PP of 20 µm allows entire surface of Moderate solvent Resistance to Some solvent -80°C freezer Re-sealable with -80°C freezer caps in a foil frame Resistant to DMSO Resistant to DMSO Resistant to DMSO gaseous exchange & seal makes this Re-sealable with Re-sealable with limits evaporation permeable to gases resistance DMSO resistance Moderate another Universal High resistance Can be resealed another Pierce Heat another Pierce Heat Colour print and other solvents resistance to Peel Heat Seal to solvents even Gas permeability: Good solvent Seal Seal identifies non- 3 2 solvents at room at elevated resistance, sealing surface 180 m /m /day temperature temperatures Colour print Colour print including DMSO identifies non- identifies non- Moisture vapour sealing surface sealing surface transmission: 20g/m2/day Seal Integrity Min Temperature -80°C -80°C -20°C -80°C -80°C -80°C -80°C -195°C -20°C -20°C -20°C -20°C -200°C -20°C -80°C Seal Integrity Max Temperature 80°C (or 110°C 110°C 80°C (or 110°C 80°C (or 110°C 90°C (or 110°C 90°C (or 110°C 40°C 120°C 120°C 120°C 110°C 110°C 110°C 80°C 100°C with pressurised with pressurised with pressurised with pressurised with pressurised material integrity (not heated PCR lid) heated PCR lid) heated PCR lid) heated PCR lid) heated PCR lid) seal) Pierceable ( ) Peelable RNase/DNase free Material Laminate Polymer Polymer Polymer Laminate Laminate Laminate Laminate Foil Foil Foil Foil Laminate Woven material Laminate Seals to PP, PE, PS, PC, COC PP PP, PE, PS, COC PP, PS, COC PP, COC PP, PE, PS, PC, COC PP, PE, COC PP PP, PS PP, COC PP, PS PP, PS, PC PP PP, PS, COC PP, PE, PS, COC Sealing parameters 175-185°C 175-185°C 165-175°C 175-185°C 175-185°C 175°C 175-185°C 175°C 160-175°C 170-180°C 165-180°C 165-180°C 170-180°C 170°C 175-185°C with 96 Well PP Plates 2-3 s 2-3 s 3 s 2-3 s 3 s 2 s 3 s 3 s 2 s 2 s 2 s 2 s 2-3 s 2 s 2-3 s Sealing parameters 165-180°C 170-175°C 165-175°C 165-180°C 170-175°C 175°C 170-175°C N/A 160-175°C 170-180°C 165-175°C 165-175°C 160-170°C 170°C 165-180°C with 384 Well PP Plates 3 s 2-3 s 2 s 3 s 2-3 s 2 s 2-3 s 2 s 2 s 2-3 s 2-3 s 2 s 2 s 3 s Sealing parameters 185-200°C N/A 175-185°C 185-200°C N/A 180°C N/A N/A 185-200°C 180-200°C 185-200°C 185-200°C N/A 170°C 185-200°C with Vision Plates™ 3 s 2-3 s 3 s 2 s 3 s 3 s 3 s 3 s 2 s 3 s

Product Codes/Instrument Compatibility Compatible with 4titude® a4S Automatic Roll Heat Sealer | Thermo Fisher ALPS 300™ and ALPS 3000™ | KBiosystems Wasp™ and Chameleon™ | KBioscience FlexiSeal and Cube Roll, 78 mm width 4ti-0540 4ti-0573 4ti-0580 4ti-0549 4ti-0520 4ti-0523 4ti-0585 4ti-0530 4ti-0538 4ti-0535 4ti-0545 4ti-0590 4ti-0598 4ti-0540/SLIT Roll, 78 mm width, short roll* 4ti-0540/80 4ti-0573/122 4ti-0580/122 4ti-0520/122 4ti-0585/100 4ti-0530/122 4ti-0535/122 4ti-0545/122 4ti-0590/100 4ti-0598/122 Sample roll, 78 mm width 4ti-0540S 4ti-0573S 4ti-0580S 4ti-0549/S 4ti-0520S 4ti-0523S 4ti-0585S 4ti-0530S 4ti-0538S 4ti-0535S 4ti-0545S 4ti-0590S 4ti-0598S 4ti-0540/SLIT/S Compatible with Agilent (Velocity 11) PlateLoc® Roll, 115 mm width 4ti-0542 4ti-0574 4ti-0582 4ti-0548 4ti-0522 4ti-0524 4ti-0586 4ti-0532 4ti-0539 4ti-0537 4ti-0546 4ti-0592** 4ti-0599 Sample roll, 115 mm width 4ti-0542S 4ti-0574S 4ti-0582S 4ti-0548/S 4ti-0522S 4ti-0524S 4ti-0586S 4ti-0532S 4ti-0539S 4ti-0537S 4ti-0546/S 4ti-0592S** 4ti-0599S Compatible with REMP Portrait Heat Sealer (PHS) Roll, 78 mm width, large core 4ti-0540/REMP 4ti-0585/REMP 4ti-0530/REMP 4ti-0535/REMP Compatible with REMP Landscape | Stacking Heat Sealers (LHS / SHS) Roll, 115 mm width, large core 4ti-0542/REMP 4ti-0586/REMP 4ti-0532/REMP 4ti-0537/REMP Compatible with 4titude® 4s3™ Semi-Automatic Sheet Heat Sealer | Thermo Fisher ALPS™ 25 and ALPS™ 50 | KBiosystems E-Fly 2 | REMP EasySealer Sheets 4ti-0541 4ti-0575 4ti-0581 4ti-05481 4ti-0521 4ti-05231 4ti-0587 4ti-0543 4ti-0531 4ti-05381 4ti-0536 4ti-0547 4ti-0591 4ti-0597 4ti-0541/SLIT Compatible with 4titude® 4s3™ Semi-Automatic Sheet Heat Sealer Random Access, sheets 4ti-05381/RA Compatible with 4titude® a4SRA Automatic Random Access Heat Sealer Random Access, roll, 100 mm width 4ti-0539/RA

* For use with the 4titude® a4S Automatic Roll Heat Sealer when using lower roll position and the optional dust cover for protection of the roll · ** NOT compatible with Agilent (Velocity 11) PlateLoc® 4titude® recognises that designated trademarks and brands of the Instrument Compatibility Table are the property of their respectice owners.

10 | Contact us: [email protected] For more information see www.4ti.co.uk/seal Heat Sealing Consumables Comparison & Instrument Compatibility Table

For more information see www.4ti.co.uk/seal Contact us: [email protected] | 11 Random Access Heat Sealing FrameSeal™ & Thermal Test Film

Random Access Product Range FrameStar® is our superior technology of making PCR plates with ultra-thin polypropylene wells fitted into a robust polycarbonate frame that provides excellent stability. Our Random Access plates develop this technology further to supply a novel 96 well plate with individually removable wells combining both flexibility and robustness. Each well is moulded into a rigid frame suitable for use with automation. For details on our FrameStar® and Random Access product range please see www.4ti.co.uk. Figure 1: Random Access 96 Well Skirted PCR Plate

Manual Random Access Heat Sealing The 96 well Random Access plate can be sealed in one step using Random Access Pierce Seal Strong, 96 individual foil seal spots on a removable backing (4ti- 05381/RA, see page 8). These seals result in individually sealed tubes that are pierceable, allowing for sample access. Random Access Heat Seals are currently available in sheet format for use with the 4s3™ Semi-Automated Sheet Heat Sealer (using the 4ti-0613 Random Access Figure 2: Random Access Sealing Procedure adapter). using the 4s3™ Semi-Automatic Sheet Heat Sealer (4ti-0655)

Automatic Random Access Heat Sealing The roll-fed a4SRA Automatic Random Access Heat Sealer allows for automatic sealing of Random Access plates using Random Access Heat Seal rolls. The roll with the indexed groups of 96 individual sealing discs is automatically fed through the heat sealer. The accurate sealing is controlled by a sensor which gets activated by optical windows in the material feed, but can also by adjusted. Sealing temperature, time of sealing and exit delay (for cooling) can be controlled via the instrument's touchscreen. Figure 3: Random Access Sealing Procedure using the a4SRA Automatic Random Access Custom versions of instrument and sealing Heat Sealer (4ti-0675, prototype shown) material are possible.

Manual Random Access Heat Sealing Automatic Random Access Heat Sealing

Code Details Qty Code Details Qty 4ti-0655 4s3™ Semi-Automatic Sheet Heat Sealer 1 4ti-0675 a4SRA Automatic Random Access Heat Sealer 1 4ti-0613 4s3™ Plate Support Adapter, Random Access, 1 4ti-0539/RA Random Access Pierce Heat Seal Strong, 1 for low profile plates roll (420 m x 100 mm, approx. 3,200 sealed PCR 4ti-0614 4s3™ Plate Support Adapter, Random Access, 1 plates) for standard profile plates 4ti-0960/RA Random Access 96 Well Skirted PCR Plate, 50 4ti-05381/RA Random Access Pierce Heat Seal Strong, 100 low profile, clear wells white frame sheets, (127 mm x 100 mm) 4ti-0960/RA Random Access 96 Well Skirted PCR Plate, 50 low profile, clear wells white frame

12 | Contact us: [email protected] For more information see www.4ti.co.uk/seal Random Access Heat Sealing FrameSeal™ & Thermal Test Film

Random Access Product Range FrameSeal™ FrameStar® is our superior technology of making PCR plates with ultra-thin Our FrameSeal™ utilises a sheet of standard 4titude® heat sealing material mounted onto a disposable, rigid, stackable plastic polypropylene wells fitted into a robust polycarbonate frame that provides excellent frame. A FrameSeal™ can be placed either manually, or with a robotic gripper, onto a wide range of skirted and semi-skirted stability. Our Random Access plates develop this technology further to supply a microplates (including PCR plates) and is compatible with our 4s3™ Semi-Automatic Sheet Heat Sealer (see page 16). After novel 96 well plate with individually removable wells combining both flexibility and sealing, there is the option to remove the frame by tearing along pre-cut lines to leave the heat seal in place on the plate surface. robustness. Each well is moulded into a rigid frame suitable for use with automation. For details on our FrameStar® and Random Access product range please see www.4ti.co.uk. Figure 1: Random Access 96 Well Skirted FrameSeal™ PCR Plate Heat sealing sheets held within a disposable, rigid, stackable frame, suitable for automation Manual Random Access Heat Sealing Code Details Frames/Case 4ti-0541/FS Clear Heat Seal FrameSeal™ (see page 5) 50 The 96 well Random Access plate can be sealed in one step using Random Access 4ti-0575/FS Clear Weld Heat Seal Mark 2 FrameSeal™ (see page 5) 50

Pierce Seal Strong, 96 individual foil seal spots on a removable backing (4ti- Please refer to the corresponding product page to see all features and specifications 05381/RA, see page 8). These seals result in individually sealed tubes that are of the respective heat sealing material. pierceable, allowing for sample access. Features Random Access Heat Seals are currently available in sheet format for use with the • Easy handling compared to sheet or roll formats 4s3™ Semi-Automated Sheet Heat Sealer (using the 4ti-0613 Random Access Figure 2: Random Access Sealing Procedure • Perforated for easy removal following heat sealing adapter). using the 4s3™ Semi-Automatic Sheet Heat • Allows for the use of sheeted sealing material in automation Sealer (4ti-0655)

Automatic Random Access Heat Sealing The roll-fed a4SRA Automatic Random Access Thermal Test Film Heat Sealer allows for automatic sealing of ® Random Access plates using Random Access 4titude 's Thermal Test Film was developed to check the uniformity and reproducibility of a heat sealer's heating block. Heat Seal rolls. The film features a thermosensitive colour-forming layer plus a protective layer, both attached to a base material. Depending on The roll with the indexed groups of 96 the temperature applied to the film, a colour is produced in varying density and hue – giving a perfect image of heat distribution individual sealing discs is automatically fed across the heating block of your heat sealer. through the heat sealer. The accurate sealing is controlled by a sensor which gets activated Thermal Test Film by optical windows in the material feed, but Thermosensitive colour-forming film, for evaluation of consistent temperature across can also by adjusted. Sealing temperature, a heating block time of sealing and exit delay (for cooling) Code Details Sheets or Roll/Case can be controlled via the instrument's 4ti-0640 Sheets (125 x 80 mm) 25 touchscreen. 4ti-0641 Starter Kit, contents: sheets (125 x 80 mm) and 1 silicone pad 10 RA Figure 3: Random Access Sealing Procedure using the a4S Automatic Random Access 4ti-0642 Roll Kit, contents: roll (1 m x 80 mm) and 1 silicone pad 1 Custom versions of instrument and sealing Heat Sealer (4ti-0675, prototype shown) material are possible. Features • To effectively test the temperature of a heat sealer's heating Thermal Test Film Colour Chart block between 160 – 200°C Manual Random Access Heat Sealing Automatic Random Access Heat Sealing • Colour change varies according to dwell time and temperature Code Details Qty Code Details Qty • The shorter the duration, the paler and more blueish the 4ti-0655 4s3™ Semi-Automatic Sheet Heat Sealer 1 4ti-0675 a4SRA Automatic Random Access Heat Sealer 1 colour; The longer the duration, the more saturated and reddish the colour, see colour chart right 4ti-0613 4s3™ Plate Support Adapter, Random Access, 1 4ti-0539/RA Random Access Pierce Heat Seal Strong, 1 for low profile plates roll (420 m x 100 mm, approx. 3,200 sealed PCR • Available in sheet and roll format to be used with sheet fed 4ti-0614 4s3™ Plate Support Adapter, Random Access, 1 plates) and roll fed heat sealers Note: This colour chart is just an example based on the results of the tests performed for standard profile plates 4ti-0960/RA Random Access 96 Well Skirted PCR Plate, 50 • For details, please refer to the corresponding webpage in our laboratories. Before using the Thermal Test Film, a similar chart needs to be low profile, clear wells white frame 4ti-05381/RA Random Access Pierce Heat Seal Strong, 100 created that is based on your actual measurement conditions sheets, (127 mm x 100 mm) 4ti-0960/RA Random Access 96 Well Skirted PCR Plate, 50 low profile, clear wells white frame

For more information see www.4ti.co.uk/seal Contact us: [email protected] | 13 a4S Automatic Roll Heat Sealer Providing the perfect solution for automated heat sealing

a4S Automatic Roll Heat Sealer The main advantage of the a4S is that it is independent from an external air supply, which gives it the flexibility to be used on standard lab benches as a stand-alone unit, or integrated within a robotic set-up. The sealer’s rapid heating element enables a fast start-up, and the stand-by function saves energy when the unit is not in use. The a4S sealer is compatible with a wide range of SBS footprint plates, including all PCR plate formats from 96 to 1536 wells, assay plates, deep well storage plates and microplates. Varying plate heights, from 3 mm to 60 mm can be accommodated, with minimal adjustment of the instrument. Plates are presented in the portrait format and the plate position on the shuttle allows good access for robotic integration up to roll change.

• Unrivalled sealing performance and consistency 5,000 perfect seals without intervention • SiLA compatible Easy integration into robotic systems • No external air supply required Cost-saving and compact device, simple installation

Features

Installation • No need for compressed air — installation is fast and flexible S I M N P IO T LE A • 2 positions for different roll sizes — increased flexibility INSTALL • Optional roll cover — seal protection for sensitive applications Figure 4: Optional roll cover to • Special short roll formats available when using lower roll position and the protect the seal roll optional dust cover for protection of the roll

Handling • Colour touch screen with intuitive user interface — ease of use

E A G • Unlimited password protected protocols — 3 security levels save personalised S IN Y H DL AN and SOP set temperatures and times

• Rapid heating — fast start up time, block uniformity is maintained to ± 1°C Figure 5: Colour touch screen • Variable time/temperature controls — adjustment of time and temperature with intuitive user interface - ease of use settings enables the perfect seal to be established for any sealer friendly, high-quality plate type, e.g. all 4titude® plates • Up to 5,000 seals without manual intervention — true walk-away-system

Sealing

R • Reproducible sealing — a fixed pressure and accurate time and temperature

E G P N R I O L controls ensure consistent sealing D EA UCI E S BL • High sealing pressure — improved seal uniformity on the widest range of plates Figure 6: Protocol management function - unlimited password • Sealing cycle time of less than 15 sec — time saving protected protocols

14 | Contact us: [email protected] For more information see www.4ti.co.uk/seal a4S Automatic Roll Heat Sealer Providing the perfect solution for automated heat sealing

a4S Automatic Roll Heat Sealer Flexibility The main advantage of the a4S is that it is independent from an external air supply, which gives it the • Compatible with a wide range of plate types, from racked tubes to 1536 H I Y G H IT well PCR plates — highest flexibility E L flexibility to be used on standard lab benches as a stand-alone unit, or integrated within a robotic S BI T FLEXI set-up. The sealer’s rapid heating element enables a fast start-up, and the stand-by function • Flexibility of seal material choice — 4titude® offers a wide range of sealing saves energy when the unit is not in use. materials, with a variety of properties for use with the a4S The a4S sealer is compatible with a wide range of SBS footprint plates, • Compatible with gas permeable heat seals — no instrument modification Figure 7: Compatibility with a including all PCR plate formats from 96 to 1536 wells, assay plates, deep necessary for maximum convenience wide range of plate types - well storage plates and microplates. Varying plate heights, from 3 mm to two adapters supplied for 60 mm can be accommodated, with minimal adjustment of the instrument. Economic efficiency optimal sealing results, with Plates are presented in the portrait format and the plate position on the • Competitive pricing — save money without compromises custom adapters available on E request C Y shuttle allows good access for robotic integration up to roll change. O C N N • Auto standby mode — saves energy and prolongs component life OM IE IC EFFIC • 2 year warranty on parts and labour • Unrivalled sealing performance and consistency • Extended warranty and service contracts available — for peace of mind 5,000 perfect seals without intervention • SiLA compatible Plug and Play Robotic Integration Easy integration into robotic systems All 4titude® sealing instruments are compliant with SiLA standards for rapid integration of automated systems R O N B O • No external air supply required O I (www.sila-standard.org). This means they can be “plug and play” connected with other instruments, such T T I RA Cost-saving and compact device, simple installation C INTEG as readers, robotic arms and liquid handlers, to give a custom automated system without the need to write expensive custom drivers. The a4S heat sealer is extremely versatile leaving you the freedom to expand and reconfigure your systems such as adding robotic plate handling. Features • Full communication protocol available — operate the instrument and record sealing conditions for each seal using Installation the RS232 communication port • No need for compressed air — installation is fast and flexible S I N • SiLA compatible — quick and easy integration with other SiLA M P IO T LE A • 2 positions for different roll sizes — increased flexibility INSTALL compliant devices using the SiLA driver • Optional roll cover — seal protection for sensitive applications Figure 4: Optional roll cover to • Special short roll formats available when using lower roll position and the protect the seal roll optional dust cover for protection of the roll Technical Specifications a4S Automatic Roll Heat Sealer Parameter Specification Includes: Power cord, manual, plate support adapters A (4ti-0665-2) and B (4ti-0665-3), 24 months parts and labour warranty Handling Dimensions (W x L x H) 230 x 507 x 276 mm Please note: Additional space is required if large seal rolls are used Code Details Qty • Colour touch screen with intuitive user interface — ease of use Sealing Temperature Range 100-195°C 4ti-0665 a4S Automatic Roll Heat Sealer 1 E A G S N • Unlimited password protected protocols — 3 security levels save personalised Y LI Sealing Time Range 0.1-10 sec HAND Accessories and SOP set temperatures and times Weight (without roll) 27 kg Code Details Qty • Rapid heating — fast start up time, block uniformity is maintained to ± 1°C Figure 5: Colour touch screen Power Supply V in: AC 100-240 V V out: DC 24 V 320 W 4ti-0665-1 a4S Seal Loading Tool 1 • Variable time/temperature controls — adjustment of time and temperature with intuitive user interface - ease of use Power Consumption 700 W (max) 4ti-0665-2 a4S Plate Support Adapter A 1 settings enables the perfect seal to be established for any sealer friendly, Working Temperature Range 10-30°C 4ti-0665-3 a4S Plate Support Adapter B 1 high-quality plate type, e.g. all 4titude® plates Operating Humidity (RH) 0-85� 4ti-0665-4 a4S Roll Holder Set 1 • Up to 5,000 seals without manual intervention — true walk-away-system Connection RS-232 serial port, USB port Contents: spindle, clamping wheels, locking nuts, O-rings 4ti-0665-5 a4S Vacuum Cups, Front, clear, set of 2 2 Sealing 4ti-0665-6 a4S Vacuum Cups, Back, black, set of 2 2 4ti-0665-8 a4S Clear Plastic Roll Dust Cover 1

R • Reproducible sealing — a fixed pressure and accurate time and temperature E G 4ti-0665-41 SiLA driver for a4S heat sealer 1 P N R I O L controls ensure consistent sealing D EA UCI E S BL • High sealing pressure — improved seal uniformity on the widest range of plates Figure 6: Protocol management Figure 8: Flexibility of seal function - unlimited password material choice - 4titude® • Sealing cycle time of less than 15 sec — time saving protected protocols offers a wide range of sealing films and foils

For more information see www.4ti.co.uk/seal Contact us: [email protected] | 15 4s3™ Semi-Automatic Sheet The 4s3™ is compatible with a wide range of seals and plates of differing designs and heights Heat Sealer

4s3™ Semi-Automatic Sheet Heat Sealer The 4s3™ is a semi-automatic sheet heat sealer which is compatible with a wide range of seals and plates of differing designs and heights. With variable temperature and time settings, sealing conditions are easily optimised to produce a 100� tight seal, eliminating sample loss. Plate and seal are placed on the holder, the "Operate" button pressed and the drawer automatically closes. The sealing process is controlled by an electric mechanism. Heat sealers provide a mechanism for controlled plate sealing, eliminating variation and giving consistent sealing every time.

• Automatic sealing process for unmatched sealing consistency • Variable temperature and time settings for easy optimisation of sealing conditions • Simple, single button operation • Rapid heating element for fast start up • Real-time temperature display • Auto stand-by function to save energy • Auto switch off function for added safety • Compatible with a wide variety of consumables • RS-232 serial port enables full integration with robotic automation systems • Full range of heat seals available • Small footprint • Low temperature sealing for use with adhesive seals Figure 9: Real-time temperature display

Features

Real Time Display • The rapid heating element allows for a quick start-up within minutes S I M N P IO T LE A • The temperature is displayed in real-time so that the heating up process can be monitored INSTALL

Energy Saving Functions • When the unit is left idle it automatically switches into standby mode, during which the temperature of the E C Y O C N N heating element is reduced to 60°C to save energy O IE MIC FIC EF • When the sealer is left idle for longer periods it switches off automatically for added safety • The times at which the unit switches into standby and switch off modes can be adjusted to suit your needs

RS-232 Port for Automation Integration • The 4s3™ has an RS-232 port to enable it to be integrated into most automated R O N B IO systems O T T A IC I GR NTE • SiLA compatible — quick and easy integration with other SiLA compliant devices using the SiLA driver

16 | Contact us: [email protected] For more information see www.4ti.co.uk/seal 4s3™ Semi-Automatic Sheet The 4s3™ is compatible with a wide range of seals and plates of differing designs and heights Heat Sealer

4s3™ Semi-Automatic Sheet Heat Sealer Adapters

R The 4s3™ employs a unique and cost saving adapter system

The 4s3™ is a semi-automatic sheet heat sealer which is compatible with a wide range of G E P N R I O L seals and plates of differing designs and heights. D EA • Deep well plates and most other skirted plates can be sealed UCIBLE S With variable temperature and time settings, sealing conditions are easily optimised on the standard plate support adapter (4ti-0615) to produce a 100� tight seal, eliminating sample loss. • For all other 96 well PCR plates, an additional plate support Figure 10: 4ti-0615 Figure 11: 4ti-0625 Plate and seal are placed on the holder, the "Operate" button pressed and the drawer adapter (4ti-0625, optional extra) must be used Plate Support Adapter, Plate Support Adapter, automatically closes. The sealing process is controlled by an electric mechanism. • Both adapters can also be combined for easier handling of Standard PCR 96 Heat sealers provide a mechanism for controlled plate sealing, eliminating variation shallow plates such as 384 well PCR plates. and giving consistent sealing every time. • For best sealing results with Random Access seals, 4 different adapters are available to offer the perfect plate support and seal alignment • Automatic sealing process for unmatched sealing consistency • Variable temperature and time settings for easy optimisation of sealing conditions Sealing Aids For better sealing of films which have a tendency to curl, • Simple, single button operation Figure 12: 4ti-0613 Figure 13: 4ti-0614 4titude® offers sealing aids. • Rapid heating element for fast start up Plate Support Adapter, Plate Support Adapter, • A weighted platen (4ti-0602) for use with deep well blocks can Random Access, Low Random Access, • Real-time temperature display Profile Standard Profile be ordered separately • Auto stand-by function to save energy • A sealing frame for use with all other plate designs is supplied • Auto switch off function for added safety when ordering the optional 4ti-0625 plate support adapter • Compatible with a wide variety of consumables • RS-232 serial port enables full integration with robotic automation systems Plate Formats • Full range of heat seals available The following plate formats can be used with the appropriate

H I Y G T Figure 14: 4ti-0602 Figure 15: 4ti-0612 • Small footprint H I plate adapter: E L S BI T F EXI Weighted Sealing Platen Sealing Frame • Low temperature sealing for use with adhesive seals Figure 9: Real-time L • Standard SBS footprint PP & PS plates & deepwell blocks temperature display • PCR plates (skirted, semi-skirted and non-skirted formats)

Features FrameSeal™ Heat Sealing Frames 4titude® offers heat sealing sheets held 4s3™ Semi-Automatic Sheet Heat Sealer Real Time Display within a disposable, rigid, stackable frame Includes: Power cord, manual, standard plate support adapter (4ti-0615), • The rapid heating element allows for a quick start-up within minutes that are compatible with the 4s3™ heat 12 months parts and labour warranty S I M N sealer. Please see page 13. P IO Code Details Qty L T • The temperature is displayed in real-time so that the heating up process can be monitored E LA INSTAL 4ti-0655 4s3™ Semi-Automatic Sheet Heat Sealer 1

Energy Saving Functions Accessories Code Details Qty • When the unit is left idle it automatically switches into standby mode, during which the temperature of the E Figure 16: FrameSeal™ C Y 4ti-0615 4s3™ Plate Support Adapter, Standard, supplied with the O C 1 N N heating element is reduced to 60°C to save energy E unit, for skirted 96 and 384 well plates OM I IC EFFIC • When the sealer is left idle for longer periods it switches off automatically for added safety 4ti-0616 4s3™ Plate Support Adapter, Roche 1536, 1 for Roche 1536 well PCR plates • The times at which the unit switches into standby and switch off modes can be adjusted to suit your needs 4ti-0625 4s3™ Plate Support Adapter, PCR 96, Technical Specifications 1 for 96 well PCR plates RS-232 Port for Automation Integration Parameter Specification 4ti-0618 4s3™ Plate Support Adapter, PCR 384, 1 for 384 well PCR plates • The 4s3™ has an RS-232 port to enable it to be integrated into most automated Dimensions (W x L x H) 181 x 275 x 310 mm R 4ti-0613 4s3™ Plate Support Adapter, Random Access, Low Profile, 1 O N Sealing Temperature Range 60-200°C B IO systems O T for low profile plates TI A C IN EGR Sealing Time Range 0-10 sec T 4ti-0614 4s3™ Plate Support Adapter, Random Access, Standard 1 • SiLA compatible — quick and easy integration with other SiLA compliant devices Weight 9 kg using the SiLA driver Profile, for standard profile plates Power Supply 110/230V 4ti-0602 4s3™ Weighted Sealing Platen 1 Power Consumption 300 W (max) 4ti-0612 4s3™ Sealing Frame, for use with Plate Support 1 Connection RS-232 serial port Adapter, PCR 96 (4ti-0625)

For more information see www.4ti.co.uk/seal Contact us: [email protected] | 17 Adhesive Sealing Consumables & Sealing Accessories

Adhesive Sealing Consumables & Sealing Accessories Adhesive seals provide a versatile, yet robust, method for protecting samples. The seals are supplied as sheets and some are also available in roll format. Most adhesive seals are supplied with convenient tabs on both ends for easy application and removal. These tabs also enable easy peeling to remove a seal without leaving adhesive residue on the sealing surface. All our seals are produced and processed under strictly controlled environmental conditions and certified free from DNase, RNase and human genomic DNA. To choose the most suitable seal, please refer to the comparison table on page 24 which describes the recommended applications and technical features of each seal. To obtain the best sealing results with adhesive seals, we strongly recommend the use of high quality plates with raised rim sealing rings for optimal sealing integrity and guaranteed flatness. All 4titude® PCR plates are designed with these features. To improve seal application by ensuring even pressure is applied, we offer a seal roller and a seal applicator, for complete and secure application of all our adhesive seals. We also offer supplementary products like the compression pad, details see page 23.

PCR Seal

* Peelable Seal Integrity -20˚C 110˚C with * pressurised Temperature Range 110˚C heated lid

PCR Seal Clear adhesive film, strong adhesive, peelable, suitable for PCR and optical applications

Code Details Sheets/Case 4ti-0500 Sheets (135 mm x 80 mm) 100 Features 4ti-0500/8 Perforated sheets (115 m x 100 mm) for tearing into 100 • Durable transparent polyester film with a strong adhesive layer 8 well strips 4ti-0500/12 Perforated sheets (137 mm x 71 mm) for tearing into 100 • Recommended for PCR, qPCR, and other optical applications 12 well strips as well as sample storage 4ti-0500/HP Sheets Half plate size (70 mm x 80 mm) 100 • Also available in two flexible formats with perforated sheets, to enable tearing into 8 well and 12 well strips, respectively • Also available as half plate seals to match the size of our FrameStar® 192 Well Semi-Skirted PCR Plate (half 384 well plate), please see www.4ti.co.uk for more details

Q-Stick™ qPCR Seal

* Peelable Seal Integrity -20˚C 110˚C with * pressurised Temperature Range 110˚C heated lid

Q-Stick™ qPCR Seal Adhesive film with 96 optically clear windows, peelable, suitable for qPCR and optical applications

Code Details Sheets/Case 4ti-0565** Sheets (133 mm x 76 mm) 100

Features **Not available for purchase in or onwards distribution to the USA • Strongest sealing integrity with optimised optical properties, thanks to 96 adhesive-free windows • Reduced contact of reagent and adhesive • Recommended for qPCR and other optical applications, such as fluorescence or colorimetric measurements

18 | Contact us: [email protected] For more information see www.4ti.co.uk/seal Adhesive Sealing | Consumables 19 | 1 1 100 100 100 100 Case Case Case / / /

Sheets Sheets [email protected]

- Sheets or Rolls Sheets Contact us: 4ti Pierceable Pierceable 0500, see page 18) ensuring the page 0500, see - Peelable C with Peelable Peelable (130 mm x 80 mm) (140 mm x 77 mm) 700 seals) (100 m x 80 mm, approx. Half plate size (70 mm x 77 mm) size Half plate (5 m x 80 mm) ˚ (137 mm x 80 mm) 110 * pressurised lid heated * C C C C activated seal. The adhesive is released when pressure is when pressure is released adhesive The seal. activated C C ˚ ˚ ˚ ˚ - ˚ ˚ 80 40 80 - - Details Details Details Sheets Sheets Sheets Roll Sheets Roll Sample - 110 110 120

HP S / / 0550 0500FL 0560 0560 0561 0561 ------applied firmly and evenly to the seal. Our Adhesive Seal Roller or Adhesive Seal Seal Adhesive Roller or Seal Adhesive Our seal. to the evenly applied firmly and our of the use recommend also We with this product. use for ideal are Applicator see details For during PCR with this product. Pad Film Compression Optical 23. page 4ti 4ti 4ti strongest adhesive seal adhesive strongest Uses the same adhesive as our PCR Seal (4ti as our PCR Seal adhesive the same Uses PCR Foil Seal PCR Foil suitable peelable, adhesive, strong foil, aluminium adhesive Pierceable applications high temperature for Code Note Technical a of the formation manner which prevents in an irregular tears the foil pierced, When vacuum Strong Seal PCR Foil high for suitable pierceable, peelable, adhesive, strong aluminium foil, Adhesive storage temperature and low incubations temperature Code Note Technical qPCR Seal for qPCR suitable peelable, adhesive, activated film, pressure adhesive clear Optically applications and optical Code Note Technical is a pressure qPCR Seal 4ti 4ti 4ti Seal Integrity Integrity Seal Range Temperature Seal Integrity Integrity Seal Range Temperature Integrity Seal Range Temperature

- suitable for for suitable - activated activated - seal / Skirted PCR Plate (half 384 PCR Plate Skirted - 192 Well Semi 192 Well ® sticky film with a strong pressure strong film with a sticky the packaging from when removed sticky - - well plate), please see our website for more details more for our website see please plate), well Pierceable aluminium foil seal with our strongest acrylic with our strongest seal aluminium foil Pierceable adhesive storage temperature PCR and low for Recommended Aluminium foil seal with a strong acrylic adhesive acrylic adhesive with a strong seal Aluminium foil integrity high of a seal producing evaporation sample prevents Effectively Non PCR and other high temperature applications high temperature PCR and other adhesive Non improved handling when wearing gloves wearing handling when improved particularly applications, for optical developed Specifically qPCR our of the size match to seals as half plate available Also FrameStar For more information see www.4ti.co.uk Features • • PCR Foil Seal Strong Seal PCR Foil Features • • PCR Foil Seal PCR Foil Features • qPCR Seal qPCR • • • 20 Adhesive Sealing | Consumables | Contact us: 4ti us: Contact • • • • Features Pierceable Film384 • • Features Pierceable Film • • Features DMSO Resistant Foil minimal pressure access to thesample wells withatiporprobe with Adhesive minimal pressure access to thesample wells withatipor probe with during piercing Adhesive Good solvent resistance, includingDMSO Peelable andpierceable filmwitha strong adhesive Adhesive Peelable andpierceable filmwitha strong adhesive Dimethyl Sulfoxide (DMSO) seal withhighlevels of solvent resistance, includingto Chemically resistant silicone adhesive layer producing a Peelable foil seal withastrong adhesive - [email protected] - - - free, cross free windows avoid cloggingof tips orneedles free, cross - - cut windows allowing for easy cut windows allowing for easy • • • • • Temperature Range Seal Integrity Temperature Range Seal Integrity Temperature Range Seal Integrity Strong adhesive seal withcross Pierceable Film Code storage Peelable adhesive foil, strong adhesive, highsolvent resistance, suitable for longterm DMSO Resistant Foil Strong adhesive seal withcross Pierceable Film384 * 4ti 4ti 4ti Code well plates (auto samplers, HPLC, sequencers) Code suitable for 384square well plates Not available for purchase inoronwards distribution to theUSA Adhesive HPLC andDNAsequencers for use inautomated systems andsample analysers suchas Developed to facilitate easy sample application orretrieval, Long can bestored for upto 5years analysers suchasHPLC andDNAsequencers Developed for use inautomated systems andsample Red adhesive for easier alignment duringseal application during piercing - - - 0512 0566 0566 - term plate storage / * 384

- free windows avoid cloggingof tips orneedles

110 120 - 80 Sheets Sheets Sheets Details Details Details - - 20 20 80 ˚ ˚ ˚ ˚ ˚ ˚ C C C C C C - (135 mmx77mm) (117 mmx80mm) (122 mmx80mm) - - samples containing <80�DMSO cut windows, peelable, pierceable, suitable for 96 cut windows, red adhesive, peelable, pierceable, Peelable Peelable Peelable For more information see www.4ti.co.uk see information more For Pierceable Pierceable Sheets Sheets Sheets / / / Case Case Case / 100 100 100 seal Adhesive Sealing | Consumables 21 | 100 100 100 Case Case Case / / / 5 x 10 5 x 10 5 x 10 10 x 10

Sheets Sheets Sheets [email protected] - Contact us: 4ti Pierceable Pierceable Peelable Peelable Peelable (135 mm x 80 mm) (135 mm x 83 mm) (140 mm x 80 mm), with 24 adhesive free free with 24 adhesive (140 mm x 80 mm), windows free with 96 adhesive (137 mm x 80 mm), windows free with 384 adhesive (137 mm x 80 mm), windows , sterile , sterile C C C C C C ˚ ˚ ˚ ˚ ˚ ˚ based material it should not be used in wet in wet be used it should not material based 20 20 - Details Details Details Sheets Sheets As above As above Sheets Sheets Sheets 0 40 80 40 - - * * *

ST ST 24 96 384 / / / / / Seal - O 0517 0518 0517 0518 0516 0516 0516 ------conditions (e.g. for shaking culture) for (e.g. conditions sterile Available Recommended for bacterial culture, particularly for long term long term for particularly culture, bacterial for Recommended is essential evaporation of prevention where incubations, sterile Available Due to its paper its Due to Not available for purchase in or onwards distribution to the USA to distribution in or onwards purchase for available Not 4ti 4ti 4ti Gas permeable adhesive seal, peelable, suitable for cell culture cell for suitable peelable, seal, adhesive Gas permeable Code Film Double Skin Breathable thin, ultra clear, optically adhesive, membrane, barrier moisture Gas permeable pierceable peelable, Code Moisture Barrier Seal 24, 96, 384 Seal Barrier Moisture peelable, windows, free with adhesive clear, film, optically adhesive Gas permeable culture cell for suitable sterile, pierceable, Code * Air 4ti 4ti 4ti 4ti Seal Integrity Integrity Seal Range Temperature Seal Integrity Integrity Seal Range Temperature Integrity Seal Range Temperature • • • • seal / term incubation, live cell assays, confocal confocal assays, cell live incubation, term - Seal - O - Thin, optically clear membrane with an adhesive layer layer with an adhesive membrane clear optically Thin, sealing effective for an effective proving whilst is gas permeable Membrane and contamination moisture to barrier Prevents evaporation and contamination whilst enabling whilst and contamination evaporation Prevents breathe to cells with culture long term enables culture, cell for Suitable evaporation reduced significantly glycerol, ethanol, detergents, DMSO, acids, to Resistant (bleach) hypochlorite and sodium isopropanol Clear film allowing for a uniform gas exchange, whilst whilst exchange, gas for a uniform film allowing Clear evaporation & preventing barrier acting as a moisture optically free, 96, or 384 adhesive with 24, Available imaging for allowing windows clear cell & bacterial eukaryotic readers, Plate Applications: long culture, microscopy Features • • For more information see www.4ti.co.uk Double Skin Breathable Film Double Skin Breathable Features • • • Air Features • • • Moisture Barrier Seal 24, 96, 384 24, Seal Barrier Moisture 22 Adhesive Sealing | Consumables | Contact us: 4ti us: Contact • • • At thekitmanufacturer • • • • At thepoint of diagnostic use • Typical Molecular Diagnostic workflows • InterSeal • Features Microplate Seal • Features The sealed plate iscut into strips withthe4titude of theseal duringtransportation (B) pierceable seal to make sure there willbenodamage The protective InterSeal isplaced ontop of the The plate (e.g. Vari After resealing, thestrips are ready for PCRanalysis need for aheat sealer (F) by applyingto theexposed blackadhesive withoutthe The strips can easily beresealed withfoil orfilm strips the seal (E) The sample isaddedto thepre pierceable seal accessible (D) The InterSeal protective layer isremoved makingthe for transport, e.g. to thepoint of diagnostic use (C) seal (e.g. Pierce Seal, 4ti The filledplate is sealed withapierceable heat on arobotic system evaporation andbest possible long Low adhesive and asacover for applications suchascentrifugation Strips™, e.g. 4ti Transparent polyester prior to PCR accessing (piercing) andresealing of strips orplates A doublesidedadhesive film to facilitate the sealing, PlateCutter (4ti - cost sealing option, suitable for temporary storage - [email protected] - - 3000) 0753 - Plate™ Frame loaded withVari - / based filmwithlow strength - C thepre - / 0530) resulting inlowest 757) isfilledwith reagents - filled strip by piercing - filled strips are ready - term storage (A) - anexample ® -

• • Temperature Range Seal Integrity Temperature Range Seal Integrity Code Low strength adhesive film,transparent, peelable, suitable for short term storage Microplate Seal suitable for re Double sidedadhesive film,black,with96holes andaprotective liner, peelable, InterSeal 4ti 4ti Code Ideal for kitmanufactures, designed to work withVari Allows flexible access to individual wells FrameStar A C E - - 0510 0519

® - sealing withouttheneedfor aheat sealer, Break 110 - - 80 Sheets Sheets Details Details 20 20 - ˚ ˚ ˚ ˚ A C C C C - Way, andTear (130 mmx80mm) (148 mmx98mm) Peelable Peelable For more information see www.4ti.co.uk see information more For - A - Way™ plates D B F - Plate™, Sheets Sheets / / Case Case / 100 100 seal Sealing | Accessories 23 | 1 1 1 1 1 Qty Qty Qty Qty stick stick -

[email protected] - 0391) to support the 0391) to 384 Holder - Contact us: 4ti Adhesive Seal Applicator Seal Adhesive Adhesive Seal Roller Seal Adhesive ® 0398 0563 0503 0391 0502 - - - - - PTFE film, to be used with adhesive seals, seals, with adhesive to be used film, PTFE lid cyclers with heated compatible Code 4ti 4ti Pierce Plate Pierce for suitable block with 96 pins, Metal or adhesive a heat of well every piercing plate 96 well sealed Code 4ti 4ti Pad Film Compression Optical a non to mat laminated foam Silicone Adhesive Seal Roller & Roller Seal Adhesive Applicator Seal Adhesive all across application seal consistent Ensure seals with adhesive use for wells, Code FrameStar Code 4ti 384 Holder (4ti ® - 0502) - 0503) - sealed 96 well PCR plate or PCR plate 96 well sealed engineered metal block with 96 pins metal engineered - - hard padded rolling wheel rolling padded hard - Machine Soft silicone foam mat laminated to a non to mat laminated foam silicone Soft Plate holder for use with 384 well plates with 384 well use holder for Plate seals adhesive of the application Improves is held a plate ensures Special design can be pressure even so that flat perfectly (4ti Roller Seal the Adhesive applied by To ensure even pressure is applied across the is applied across pressure even ensure To the plate to application complete for seal results sealing best obtain to Recommended seals when using our adhesive with a plastic, a durable Handle made of semi stick PTFE film PTFE stick without cyclers thermal Especially for lids pressurised to in a thermal cycler the plate of on top Sits seal the adhesive between adhesion improve and plate sample reduced by results Improved evaporation Convenient size for PCR plate format PCR plate for size Convenient pressure even for rigid sides Straight application aligned central to each well of a 96 well plate a 96 well of well each to aligned central or a heat of well every 96 pins pierce adhesive only) seals (pierceable microplate with a samples to access instant Enables or automated single or multichannel pipette system or Adhesive Seal Applicator (4ti Applicator Seal or Adhesive 384 well plates during seal applications, and to give a level base. a level give and to applications, during seal plates 384 well Features • Features • Features • • • Technical Note Technical or Roller Seal using the Adhesive recommend we plates, 384 well to seals applying adhesive When with our FrameStar in conjunction Applicator Seal Adhesive Features • • Roller Seal Adhesive • • • • Adhesive Seal Applicator Applicator Seal Adhesive • • • • seal / 0502 0503 - - 4ti 4ti 384 Holder ® For more information see www.4ti.co.uk Pierce Plate Pierce Optical Film Compression Pad Film Compression Optical FrameStar Adhesive Seal Roller & Adhesive Seal Applicator Seal & Adhesive Roller Seal Adhesive Adhesive Sealing Consumables Comparison Table

Clear Seals Foil Seals Cross-cut Seals Cross-cut Seals Permeable Seals Name PCR Seal Q-Stick™ qPCR qPCR Seal PCR Foil Seal PCR Foil Seal DMSO Resistant Pierceable Film Pierceable Film Moisture Barrier Seal Air-O-Seal Double Skin Microplate Seal InterSeal Name Seal Strong Foil 384 24, 96, 384 Breathabe Film Details see page 18 18 19 19 19 20 20 20 21 21 21 22 22 Details see page Specifications Specifications Application PCR qPCR, qPCR & other PCR & sample High Microplate Sample Sample Eukaryotic cell Bacterial or cell Bacteria culture Aqueous sample Diagnostic kit Application fluorescence fluorescent storage temperature sealing application or application or culture, bacterial culture storage production 96-well applications Incubations incubations & containing retrieval retrieval culture and long- microplates only Imaging low temperature solvents incl. 96 well plates 384 well plates term live assays techniques incl. storage DMSO only only Suitable for use on crystallisation plate readers Plate readers, microscopy

Special Properties Good optical Discreet optical Good optical Irregular tearing Strong adhesive High solvent Cross-cut Cross-cut Gas permeable Very low moisture Thin gas permeable, Medium strength Two sealing Special Properties clarity windows for clarity when pierced resistance reduces tip or reduces tip or that allows air and transfer rate moisture barrier transparent seal surfaces 96-well plates prevents probe becoming probe becoming CO2 exchange, but Suitable for bacterial seal Optical windows formation of clogged clogged prevents moisture or cell culture Allows air and CO vacuum evaporation 2 Good solvent Air porosity: exchange, prevents resistance, Optically clear 10 sec/100 cc/in2 evaporation including DMSO

Seal Integrity Min Temperature -20°C -20°C -80°C -40°C -80°C -20°C -20°C -80°C -20°C -20°C 0°C -20°C -20°C Seal Integrity Min Temperature

Seal Integrity Max Temperature 110°C 110°C 110°C 120°C 110°C 80°C 110°C 120°C 80°C 40°C 40°C 80°C 110°C Seal Integrity Max Temperature

Gas Permeability Rate N/A N/A N/A N/A N/A N/A N/A N/A 0.6 m3/m2/day 8,900 m3/m2/day 900 m3/m2/day N/A N/A Gas Permeability Rate

Moisture Vapour Transmission Rate N/A N/A N/A N/A N/A N/A N/A N/A 1 g/m2/day 4,200 g/m2/day 450 g/m2/day N/A N/A Moisture Vapour Transmission Rate Sterile Sterile

Pierceable Pierceable

Peelable Peelable

RNase/DNase free RNase/DNase free

Product Codes Product Codes

Code 4ti-0500 4ti-0565 4ti-0560 4ti-0550 4ti-0500FL 4ti-0512 4ti-0566 4ti-0566/384 4ti-0516/24 4ti-0517 4ti-0518 4ti-0510 4ti-0519 Code Format Sheets Sheets Sheets Sheets Sheets Sheets Sheets Sheets Sheets Sheets Sheets Sheets Sheets Format Dimensions 135 mm x 80 mm 133 mm x 76 mm 140 mm x 77 mm 130 mm x 80 mm 137 mm x 80 mm 122 mm x 80 mm 135 mm x 77 mm 117 mm x 80 mm 140 mm x 80 mm 135 mm x 80 mm 135 mm x 83 mm 130 mm x 80 mm 148 mm x 98 mm Dimensions

Code 4ti-0500/8 4ti-0561 4ti-0516/96 4ti-0517/ST 4ti-0518/ST Code Format Perforated sheets Roll Sheets Sheets, sterile Sheets, sterile Format Dimensions 115 mm x 100 mm 100 m x 80 mm 137 mm x 80 mm 135 mm x 80 mm 135 mm x 83 mm Dimensions

Code 4ti-0500/12 4ti-0561/S 4ti-0516/384 Code Format Perforated sheets Sample roll Sheets Format Dimensions 137 mm x 71 mm 5 m x 80 mm 137 mm x 80 mm Dimensions

Code 4ti-0500/HP 4ti-0560/HP Code Format Half plate sheets Half plate sheets Format Dimensions 70 mm x 80 mm 70 mm x 77 mm Dimensions

24 | Contact us: [email protected] For more information see www.4ti.co.uk/seal Adhesive Sealing Consumables Comparison Table

Seal Integrity Min Temperature -20°C -20°C -80°C -40°C -80°C -20°C -20°C -80°C -20°C -20°C 0°C -20°C -20°C Seal Integrity Min Temperature

Seal Integrity Max Temperature 110°C 110°C 110°C 120°C 110°C 80°C 110°C 120°C 80°C 40°C 40°C 80°C 110°C Seal Integrity Max Temperature

Gas Permeability Rate N/A N/A N/A N/A N/A N/A N/A N/A 0.6 m3/m2/day 8,900 m3/m2/day 900 m3/m2/day N/A N/A Gas Permeability Rate

Moisture Vapour Transmission Rate N/A N/A N/A N/A N/A N/A N/A N/A 1 g/m2/day 4,200 g/m2/day 450 g/m2/day N/A N/A Moisture Vapour Transmission Rate Sterile Sterile

Pierceable Pierceable

Peelable Peelable

RNase/DNase free RNase/DNase free

Product Codes Product Codes

Code 4ti-0500/12 4ti-0561/S 4ti-0516/384 Code Format Perforated sheets Sample roll Sheets Format Dimensions 137 mm x 71 mm 5 m x 80 mm 137 mm x 80 mm Dimensions

Code 4ti-0500/HP 4ti-0560/HP Code Format Half plate sheets Half plate sheets Format Dimensions 70 mm x 80 mm 70 mm x 77 mm Dimensions

For more information see www.4ti.co.uk/seal Contact us: [email protected] | 25 Caps, Lids & Mats

Caps, Lids & Mats As an alternative to sealing films, 4titude® offers multiple types of cap strips for sealing both plates and tubes - domed, flat, strips of 8, strips of 12, and our new optically superior CrystalStrips™. A variety of rigid polystyrene lids are available for PCR plates and microplates, including lids compatible with our FrameStar®, Vision Plate™ and UltraVision™ Plate ranges. 4titude® also stock silicone sealing mats for use with our storage plate ranges and in a variety of formats depending on the well size, number and shape.

Strips of 8 Caps & Strips of 12 Caps

Strips of 8 Caps & Strips of 12 Caps Clear, PP

Code Details Strips/Case 4ti-0751 Strips of 8 Flat Optical Caps 300 4ti-0783 as above 125 4ti-0752 Strips of 8 Domed Caps 300 Features 4ti-0782 as above 125 • Precision moulded to ensure effective sealing 4ti-0788 Strips of 12 Flat Optical Caps 200 Note: Not compatible with low profile plates • Flat caps are optically clear for fluorescence detection (e.g. qPCR) • Large end tabs for easy removal

CrystalStrip™

4ti-0755 CrystalStrip™ Strips of 8 flat optical caps, crystal clear, designed for small volume applications such as low volume qPCR

Code Details Strips/Case 4ti-0751 4ti-0755 Strips of 8 Flat Optical Caps 300 4ti-0755/120 as above 120 Features • Optically superior crystal clear strips of 8 flat caps • Made of a special polymer with improved optical properties leading to high transmission rates • Reduced shrinking during heating and cooling phases - very tight, evaporation-safe fit • Best used with compression mat (see 4ti-0563, Optical Film Compression Pad) • Ideally suited for small volumes with low signal intensity such as low volume qPCR

26 | Contact us: [email protected] For more information see www.4ti.co.uk/seal Caps, Lids & Mats 27 | 50 50 50 50 80 80 50 50 50 80 80 50 100 100 100 100 Lids Lids Case / Mats [email protected] Sterile Sterile No Yes No Yes No No No No No Yes No - Plate™, Plate™, - * * Yes Yes Yes No Pierceable Yes No No Yes Yes No Yes No Cond. rings Cond. Cond. rings Cond. No Yes Yes No Contact us: 4ti Clear Clear Clear White Colour Clear P1 P1 H1 H1 / / / / temperature storage to protect protect to storage temperature - A12 H1 No Cut corner Cut Cut corner Cut H1 A1 A1 A1 A1 A1 A1 No 0120) - 0132, Way™, UltraVision™, Vari UltraVision™, Way™, - - Low - A highly durable to high temperatures to highly durable - - Low Low Ultra Profile Profile Low Low Low Low Low Low Low Low 0126, 4ti - 0120) only

- Style Style ® Stick™, Tear Stick™, - 0125) - Ltd. ® Rigid polystyrene lids for PCR plates, Vision Plates™ and assay plates assay and Plates™ Vision PCR plates, for lids Rigid polystyrene from samples protect to solution sealing a quick and easy give to Designed and evaporation contamination Mats made of silicone rubber silicone made of Mats For sealing storage plates used for high for used plates storage sealing For samples from evaporation from samples Skirted PCR Plate, ABI PCR Plate, Skirted - Features • • Features • • 0136) 0138 0289 Lids and Microplate Lids Microplate Lids and - - - 96 Well Skirted PCR Plate, Extra Rigid Extra PCR Plate, Skirted 96 Well Semi 96 Well RIG) ® ® ® 4ti 4ti 0125) only / - Not for use with 96 Round Deep Well Storage Microplate (4ti and 96 Round Well Microplate (4ti For use with use For 96 Round Deep Well Storage Microplate, For Magnetic Separators Separators Magnetic For Microplate, Storage Deep Well 96 Round (4ti (4ti Microplate Storage Deep Well 96 Round 4ti (e.g. Microplate Storage Deep Well 96 Square fit universal plates, well 96 round fit universal plates, storage 384 well and 4ti 0960 0770) - - seal / (4ti FrameStar FrameStar (4ti The majority of PCR plates available PCR plates of majority The For use with use For For use with use For The majority of PCR plates available PCR plates of majority The plates and assay Plates™ Vision plates and assay Plates™ Vision plates and assay Plates™ Vision 96 well plates and assay Plates™ Vision 96 well plates and assay Plates™ Vision 24 well plates and assay Plates™ Vision 24 well plates and assay Plates™ Vision Lids ® , 4s3™, CrystalStrips™, FrameSeal™, INDI™, Q FrameSeal™, , 4s3™, CrystalStrips™, ® 0287 0124 - - 96 NGS Lid 96 NGS 96 Lid ® ® 4ti 4ti Low Universal Lid Universal Low - PCR plates are covered by one or more of the following U.S. patents or their foreign counterparts, owned by by owned counterparts, or their foreign patents U.S. the following of one or more by covered are PCR plates Name Name Name 96 Square Well Sealing Cap Mat Cap Sealing Well 96 Square Mat Cap Sealing Well 96 Round Mat Cap Sealing Well 384 Square 96 Round Well Sealing Cap Mat Cap Sealing Well 96 Round Mat Cap Sealing Well 96 Round Microplate 24 Lid, Sterile Microplate Lid Microplate Universal Microplate 384 Lid Microplate 384 Lid, Sterile Microplate 96 Lid Microplate 96 Lid, Sterile Microplate 24 Lid Microplate Ultra Lid PCR Plate FrameStar FrameStar ® recognises that designated trademarks and brands are the property of their respectice owners. owners. their respectice of the property are and brands trademarks that designated recognises ® Strips™ and Vision Plate™ are trademarks of 4titude of trademarks are Plate™ Vision and Strips™ - 0139 0137 0138 0124 0135 0286 0290 0281 0282 0283 0284 0280 0289 0285 0288 0287 ------4ti 4ti 4ti 4ti 4ti Code 4ti PCR Plate Lids & FrameStar Lids & PCR Plate Code 4ti Microplate Lids Microplate Code 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti 4ti Vari For more information see www.4ti.co.uk License Statement and Trademarks Statement License Disclaimer: FrameStar Silicone Sealing Mats Sealing Silicone * Condensation rings * Condensation PCR Plate Lids, FrameStar Lids, PCR Plate 4titude Eppendorf AG: US Patent Nos. 7,347,977 and 6,340,589 Nos. US Patent Eppendorf AG: FrameStar 4titude® Contact Details 11.2018 OP / 0

UK (Head Office) Germany France USA / 1 4titude Ltd. 4titude Ltd. Germany 4titude France 4titude North America / The North Barn, Sickingenstr. 26 Bio-technofix T: +1 888 484 8424 Surrey Hills Business Park, 10553 Berlin 5, rue Denis Papin E: [email protected] Damphurst Lane, Wotton, Germany Z.A. La Mare Du Milieu - Lot C W: www.4ti.co.uk Surrey RH5 6QT, UK T: +49 (0)30 94400 469-0 91630 Guibeville, France T: +44 (0) 1306 884 885 F: +49 (0)30 94400 469-9 T: +33 (0) 1 55 58 15 19 F: +44 (0) 1306 884 886 E: [email protected] F: +33 (0) 1 55 58 15 24 Fotolia.com E: [email protected] W: www.4ti.co.uk E: [email protected] - W: www.4ti.co.uk W: www.bio-technofix.com

To see the network of our global distributors, please refer to our website www.4ti.co.uk. 0021 Brochure Sealing Solutions Overview en5 Overview Solutions Sealing 0021 Brochure - FL - 4ti Image credits: © kras99 © kras99 Image credits: