Heat Sealing

Heat sealing is the gold standard plate sealing method, minimising sample loss and maximising sample security, for PCR, qPCR, storage and other applications.

The sealing performance of heat seals is superior to cap, mat and adhesive sealing with clear reductions in sample loss and cross contamination. The optimized sealing performance allows use of smaller reaction volumes leading to reagent savings. Heat sealing is the most cost efficient way to seal plates for a wide range of applications. Per well comparisons show a 3 to 5 five fold reduction in costs for qPCR application (list price comparison of Clear Weld Seal against caps and qPCR seal) and at least a 50% reduction for storage solutions (list price comparison of Peel Seal against caps and PCR seal). Costs can be further reduced by changing to use of the material format, using rolls rather than sheets. The application of a heat seals is much faster than caps or adhesive seals. Typical sealing times are around 2,5 seconds. Depending on throughput the use of a semi-automatic or fully automatic heat sealer can guarantee IMAGES? a reproducible sealing quality by standardising the sealing parameters, time, temperature and pressure. Sealing performance variability between different users can be eliminated. 4titude® offers a wide choice of heat seals in both roll and sheet format. Different seals can be chosen to optimize the use depending on plate material, desired permeability or resistance, ability to peel or pierce the seal material and visualisation through the material. Please refer to the comparison table on page 73 for an overview of our heat sealing range. All seals are certified free from nucleases and human genomic DNA contamination. The best sealing results can be obtained by using flat, rigid plates with a raised rim design like our FrameStar® 2-component plates and reliable, high quality sealing instruments such as the 4s3 semi-automated heat sealer or the 4AS sealer. For details of the instruments see page 62 and 64. For a demonstration or trial of a heat sealer please contact us at [email protected].

+44 (0)1306 884885 www.4ti.co.uk | 61 66 66 NEW Heat Sealing | www.4ti.co.uk Now also available asFrameSeal product see page 75 Sample can beaccessed ifrequired usingabladeorneedle. long term storage. is difficult to pierce. The seal hasagoodsolvent resistance for safe permanent bondto polypropylene plates thatcan not bepeeledand Clear weld seal isan optically clear polymerfilmwhichforms a Clear WeldSealMarkII Available assheets oronaroll for automated sealers. including polypropylene, polyethylene, polystyrene andCOC plates. applications. The clear seal can beused to seal most plate types yet PCR/qPCR tight seal. Its optical clarity allows use for alloptical Clear seal isanoptically clear filmwhichforms aneasily peelable Clear Seal t-52RM 500m 4ti-0542/REMP t-54 Trial 4ti-0574S t-50RM 500m 4ti-0540/REMP t-55F Sealing 100 4ti-0575/FS 4ti-0575 t-50 500m 4ti-0540 Code t-53 610m 4ti-0573 Code t-54 500m 4ti-0574 350m 4ti-0542 t-53 Trial 4ti-0573S t-50 Trial 4ti-0540S t-51 100 Trial 4ti-0541 4ti-0542S PackSize/Dimensions PackSize/Dimensions sheets sheets roll roll roll roll x x x x x x 78mm Frame: 2 1 1 115mm 115mm 78mm 78mm 115mm

5m 5m 5m (125mm (125mm x78mm x x roll 50 78mm roll roll roll roll roll 115mm 1

2 3 1 seals/frame 2 4

Approx

x x Approx Approx Approx Approx 78mm) 78mm) Approx 5000 4200 4400 4200

6250 6250 5m per seals x115mm seals pack seals seals seals seals

• • • Applications • • • • • Applications • • • • qPCR water PCR applications and hazardous Storage Free format Available Recommended human applications qPCR or Free for Short human Available Suitable colorimetric automated

PCR applications *Once autoclaved, not recommended for *(110C withpressurised heated lid)

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N I N I

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COC = Olefin Copolymer Cyclic 3 1 2 4

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5000 5000 4200 4200 6200 6200 6200 6200 seals seals seals seals seals seals seals seals

• • • Applications • • • Applications • • • Free format Available Low human deterioration temperature 100% and compound Low PCR Sample and compound Free format Available human

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COC = Olefin Copolymer Cyclic 3 1 2 4

sheets

seals seals seals seals 6200 sealing 6200 5000 5000 tube 78mm) Approx Approx Approx Approx x

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sheets Seal ™ Size/Dimensions Pack (125mm Size/Dimensions Pack 4ti-0537S Trial 4ti-0535S Trial 4ti-0537 500m Code 4ti-0535 610m 4ti-0535/REMP 610m 4ti-0536 100 4ti-0537/REMP 610m

Code 4ti-0543 INDI +44 (0)1306 884885 884885 +44 (0)1306 and can plates PP and PS to that seals seal An aluminium heat hand. Good solvent tip or peeled by with a pipette be pierced is seal an existing onto Resealing including DMSO. resistance for surface the non-sealing identifies print colour A red possible. application. of ease Foil Seal polypropylene to that seals seal heat a foil-based is INDI Seal The of consists seal The storage. temperature low for and is suitable is frame The frame. in a foil caps individual sealing perforated 96 with an individual air well each leaving sealing heat after removed hand or with by removal easy for tabs have seals The seal. tight residue. any without leaving forceps, can plates some racks in cluster with tubes use for Mainly developed be sealed. also possible. are and piercing Re-sealing 96 INDI 70 70 Heat Sealing | www.4ti.co.uk of sample protection. Goodsolvent resistance includingDMSO. give avery strong butpeelableseal. This seal affords ahighdegree This isaheavy duty laminate material thatseals to polypropylene to Thermal Bond onto anexisting seal ispossible. and hasagoodsolvent resistance, includingto DMSO. Resealing polystyrene plates. The seal can also bepierced withapipette tip The polystyrene foil seal creates astrong, butpeelableseal to Polystyrene FoilSeal t-51 100 4ti-0591 t-50 Trial 4ti-0590S Code t-50 500m 4ti-0590 Code 100 4ti-0547 t-52 300m 4ti-0592 PackSize/Dimensions PackSize/Dimensions sheets sheets roll x x 1 115mm 78mm 5m (125mm (125mm x 78mm roll roll 1 2

x x Approx Approx 78mm) 78mm) 4200 3700 5m x seals seals 78mm

• • • • Applications • • • Applications • • • Free formats Suitable PCR and storage Long human Available thermal Storage at Low human Free Resealing compound Short-term compound low

alkalines

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1 Seal Seal Seal Seal roll Seal Seal roll sheets) seals seals 10 (125mm x 115mm 78mm 5000 6200

x x Permeable Permeable Permeable Permeable sheets Permeable Permeable packs (10 Size/Dimensions Pack Size/Dimensions Pack Approx Approx

4ti-0597/ST Gas 4ti-0599/ST Gas

4ti-0598/ST Gas Code 4ti-0598 Gas Code 4ti-0525 610m 4ti-0599 Gas 4ti-0597 Gas 4ti-0526 500m 4ti-0527 100 +44 (0)1306 884885 884885 +44 (0)1306 Gas than the discontiuned size a smaller pore has seal heat This exchange, gaseous for allows which still (4ti-0596) Seal Permeable is improved. sealers Handling in automated evaporation. but limits and is culture cell for Mk2 is suitable Seal Heat Permeable Gas The this application. for product as sterile available Gas Permeable Seal Mk 2 Gas Permeable Seal surface on the sealing which is coated seal heat is a foil-based This with light use for black pigment autofluorescence with a low a forms Seal Black The assays. fluorescent or for samples sensitive plates. polystyrene to peelable seal Black Seal Black 72 72 Heat Sealing | www.4ti.co.uk or insects. Permeable Heat Seal itcan beused for storage e.g. for seeds Due to its widertemperature tolerance compared to theGas entire widthof theroll orsheet, allowing gaseous exchange. and moderate solvent resistance. The filmhas3mmslits across the polyethylene, polystyrene andCOC plates withgoodoptical clarity This isaclear polymerfilmforming apeelableseal to polypropylene, Gas PermeableClearSeal t-51SI 100 4ti-0541/SLIT t-50SI 480m 4ti-0540/SLIT Code

PackSize/Dimensions sheets x 78mm (125mm roll 1

x Approx 78mm) 4000 seals

COC = CyclicCopolymerOlefin= COC 2 1

/ KBiosystems/ Wasp Compatible with Agilent (VelocityAgilent Compatiblewith PlateLoc 11) 300 ALPS Fisher Thermo Compatiblewith • • • • Applications

Free format Available Seed Insect human

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Heat Sealing Films & Foils Comparison Table 73

Clear Seal Clear Weld Seal Clear Seal Peel Seal DMSO Resistant Peel Pierce Seal Foil Seal & PS Foil Thermal Bond Black Seal Gas Permeable Gas Permeable INDI Seal Mark II 3730 Seal Seal Seal Mk 2 Clear Seal

p66 p66 p67 p67 p68 p68 p69/70 p70 p71 p71 p72 p69

Application qPCR, PCR (esp. water qPCR and Low temperature, Low temperature / PCR, compound Low temperature Low temperature Fluorescent Cell culture, over Storage e.g. Compound short term for use with & other light night incubation, for seeds or storae, compound bath), qPCR. ABi 3730 compound storage, room temperature storage, sample compound transportation & sensitive seed and insect insects sample storage,short-term storage. Sequencer. assays storage shipping | www.4ti.co.uk Storage & disposal short term room compound storage shipping room temperature storage. PCR, esp. of hazardous water bath cyclers. materials. temperature with DMSO & other compound storage, PCR. Storage of compound storage organic solvents. organicsolvents, acids & alkalines. (<5 days), PCR.

Special Good optical Good optical Good optical Can be peeled Can be peeled Easily pierceable. Re-sealable with Very strong seal A foil-based Small pore size 3mm slits 96 perforated Properties clarity. clarity & clarity, with PP. Resistant heat seal, that allows gaseous across entire individual directly from -80°C directly from -80°C Resistant to DMSO. another Foil Seal. to DMSO and other coated on the exchange, but surface of seal sealing caps Moderate resistant to DMSO. some solvent Resistant to DMSO. solvents sealing surface limits evaporation. makes this in a foil solvent freezer. Moderate freezer. High Re-sealable with with a low Permeability rate permeable to frame. Can resistance. resistance. Colour print fluorescence >20g/sqm/24h gases be resealed resistance to resistance to solvents another Pierce Seal. identifies non- even at elevated black pigment sealing surface. (Foil 20µm pore size solvents at room temperatures. Colour print Seal only) identifies non- temperature. sealing surface

Min Temp -80°C -80°C -20°C -80°C -80°C -20°C -20°C -200°C -80°C -20°C -80°C -195°C

Max Temp 80°C (or 110°C 80°C (or 90°C (or 110°C with 40°C 120°C 110°C 110°C 120°C 80°C 100°C 120°C 110°C with 110°C with pressurised PCR pressurised pressurised heated lid) PCR heated lid) PCR heated lid)

Piercable • • • • • •

Peelable • • • • • • • • •

RNase / DNase • • • • • • • • • • • free

Material Laminate Laminate Polymer Laminate Laminate Foil Foil Laminate Foil Woven material Laminate Laminate

Seals to PP, PE, PS, PP PP, PE, PS, PP, COC PP, PE PP, PS PP, PS PP PS PP, PS, COC PP, PE, PS, COC PP COC COC

4s3 sealing time 175-185 / 2-3 175-185 / 2-3 165-175 / 3 175-185 / 3 175-185 / 3 160-175 / 2 165-180 / 2 170-180 / 2-3 - 170 / 2 175-185 / 2-3 175 / 3 with 96well PP Plates (°C/ secs)^

4s3 sealing time 165-180 / 3 170-175 / 2-3 165-175 / 2 170-175 / 2-3 170-175 / 2-3 160-175 / 2 165-175 / 2-3 160-170 / 2 - 170 / 2 165-180 / 3 - with 384well PP Plates (°C/secs)^

4s3 sealing time 185-200 / 3 - 175-185 / 2-3 - - 185-200 / 3 185-200 / 3 - 185-200 / 3 170 / 2 185-200 / 3 - with Vision Plates (°C /secs)^

Product Codes

Roll 78mm1 4ti-0540 4ti-0573 4ti-0580 4ti-0520 4ti-0585 4ti-0530 4ti-0535 4ti-0590 4ti-0525 4ti-0598 4ti-0540/SLIT -

Roll 78mm3 4ti-0540/ 4ti-0573/REMP 4ti-0580/ 4ti-0520/REMP 4ti-0585/REMP 4ti-0530/REMP 4ti-0535/REMP 4ti-0590/REMP 4ti-0525/REMP 4ti-0598/REMP - - REMP REMP

Roll 115mm2 4ti-0542 4ti-0574 4ti-0582 4ti-0522 4ti-0586 4ti-0532 4ti-0537 4ti-0592 4ti-0526 4ti-0599 4ti-0542/SLIT -

Roll 115mm4 4ti-0542/ 4ti-0574/REMP 4ti-0582/ 4ti-0522/REMP 4ti-0586/REMP 4ti-0532/REMP 4ti-0537/REMP 4ti-0592/REMP 4ti-0526/ 4ti-0599/REMP - - REMP REMP REMP

Pack of 100 sheets 4ti-0541 4ti-0545 4ti-0581 4ti-0521 4ti-0587# 4ti-0531 4ti-0536 4ti-0591 4ti-0527 4ti-0597 4ti-0541/SLIT 4ti-0543 (125mm x 78mm)

# Supplied on a perforated roll ^ Add 1 second to the sealing time when a weighted platen is used (4ti-0602) PP Polypropylene NB Some seals available as sterile on request 1 Compatible with Thermo Fisher ALPSs 300™ / ALPS3000™ / KBiosystems Chameleon™ / KBioscience FexiSeal & Remp/Tecan Plate Sealer PS Polystyrene Rolls are also available on 150mm core for Remp/Tecan 2 Compatible with Agilent (Velocity 11 )PlateLoc® 3 Compatible with REMP Portrait Heat Sealer (PHS0) COC Cyclic Olefin Copolymer sealers. Please enquire. 884885 +44 (0)1306 4 Compatible with REMP Landscape/Stacking Heat Sealers (LHS/SHS) PE Polythene 74 74 Heat Seal Selection Table | www.4ti.co.uk Take anote of theproduct code. down to thecorrect row for your heat sealer, listed ontheleft. Choose whichtype of seal you require across thetop andfollow your sealer: This table makes itsimplerfor you to select theright heat seal for Heat SealSelectionTable Clear Seal Clear Clear Seal Peel Seal DMSO Pierce Foil Seal PS Foil Thermal Black Gas Gas INDI Seal Weld Seal 3730 Resistant Seal Seal Bond Seal Permeable Permeable Mark II Peel Seal Seal Mk 2 Clear Seal

p66 p66 p67 p67 p68 p68 p69 p70 p70 p71 p71 p72 p69

4titude

4s3 4ti-0541 4ti-0575 4ti-0581 4ti-0521 4ti-0587 4ti-0531 4ti-0536 4ti-0547 4ti-0591 4ti-0527 4ti-0597 4ti-0541/SLIT 4ti-0543

A4S 4ti-0540 4ti-0573 4ti-0580 4ti-0520 4ti-0585 4ti-0530 4ti-0535 4ti-0545 4ti-0590 4ti-0525 4ti-0598 4ti-0540/SLIT -

Agilent

PlateLoc 4ti-0542 4ti-0574 4ti-0582 4ti-0522 4ti-0586 4ti-0532 4ti-0537 - 4ti-0592 4ti-0526 4ti-0599 - -

KBioscience

FlexiSeal 4ti-0540 4ti-0573 4ti-0580 4ti-0520 4ti-0585 4ti-0530 4ti-0535 - 4ti-0590 4ti-0525 4ti-0598 4ti-0540/SLIT -

KBiosystems

E-Fly 2 4ti-0541 4ti-0575 4ti-0581 4ti-0521 4ti-0587 4ti-0531 4ti-0536 4ti-0547 4ti-0591 4ti-0527 4ti-0597 4ti-0541/SLIT 4ti-0543

Wasp 4ti-0540 4ti-0573 4ti-0580 4ti-0520 4ti-0585 4ti-0530 4ti-0535 - 4ti-0590 4ti-0525 4ti-0598 4ti-0540/SLIT -

Chameleon™ 4ti-0540 4ti-0573 4ti-0580 4ti-0520 4ti-0585 4ti-0530 4ti-0535 - 4ti-0590 4ti-0525 4ti-0598 4ti-0540/SLIT -

REMP

EasySealer 4ti-0541 4ti-0575 4ti-0581 4ti-0521 4ti-0587 4ti-0531 4ti-0536 4ti-0547 4ti-0591 4ti-0527 4ti-0597 4ti-0541/SLIT 4ti-0543

Portrait 4ti-0540 4ti-0573 4ti-0580 4ti-0520 4ti-0585 4ti-0530 4ti-0535 - 4ti-0590 4ti-0525 4ti-0598 4ti-0540/SLIT -

Thermo Fisher

ALPS 300™ 4ti-0540 4ti-0573 4ti-0580 4ti-0520 4ti-0585 4ti-0530 4ti-0535 - 4ti-0590 4ti-0525 4ti-0598 4ti-0540/SLIT -

ALPS 3000™ 4ti-0540 4ti-0573 4ti-0580 4ti-0520 4ti-0585 4ti-0530 4ti-0535 - 4ti-0590 4ti-0525 4ti-0598 4ti-0540/SLIT -

ALPS™ 50 4ti-0541 4ti-0575 4ti-0581 4ti-0521 4ti-0587 4ti-0531 4ti-0536 4ti-0547 4ti-0591 4ti-0527 4ti-0597 4ti-0541/SLIT 4ti-0543

ALPS™ 25 4ti-0541 4ti-0575 4ti-0581 4ti-0521 4ti-0587 4ti-0531 4ti-0536 4ti-0547 4ti-0591 4ti-0527 4ti-0597 4ti-0541/SLIT 4ti-0543

All roll seal formats are available in a trial roll. Add S to the end of the product code to order +44 (0)1306 884885 If you would like assistance in choosing the correct seal for your application and heat sealer, please contact technical support on +44 (0) 1306 884885 or [email protected] NEW FrameSeal™

Frame SealTM utilises a sheet of standard 4titude® heat seal material mounted onto a disposable, rigid, plastic frame. A FrameSealTM can be placed either manually or with a robotic Key advantages gripper onto a wide range of skirted and semi-skirted microplates • Key advantages compared to sheets including PCR plates and are compatible with the 4S3 heat of sealing film or roll material, are sealer. After sealing, there is the option to remove the frame by the ease of handling and the ability tearing along pre-cut lines leaving the heat seal in place on the to sterilise and improve the quality plate surface. control of every single seal. • The FrameSealTM also enables the use of Code Description Qty sheeted sealing material in automation. 4ti-0575/FS Clear Weld Seal Mk II FrameSealTM, stackable heat seal frames 50

COMING TM SOON 4ti-0541/FS ClearSeal FrameSeal 50 COMING TM SOON 4ti-0531/FS Pierce Seal FrameSeal 50

Application example: Our Clear Weld Seal Mk II (4ti-0575) heat sealing film is now available pre-mounted to create a FrameSeal. Together with Fraunhoffer IPA Institute FrameSealTM has been used in a fully integrated solution for sealing 1536well PCR plates before analysis of RNAi screening with a Roche Lightcycler1536.

Using a robotic arm, a single FrameSealTM is placed onto a 1536well PCR plate. By adjusting the position of the robot gripper, the plate and FrameSealTM combination are then transferred to the draw of a 4S3 Heat Sealer which seals the part via a software command 4s3™ shown next to the De-Framer through its RS232 port.

After the sealing process has been carried out, the sealed plate is transferred to the De-Framer, which seperates the frame from the film at each point along the edge of the film. The frame can then be removed leaving the sealed plate to be placed in the cycler.

Together the heat sealer and the FrameSealTM presented here are:

• The most cost effective automated heat sealing solution available • An approach in line with demands of diagnostics for a sterile, single‑use consumable • The most compact instrument for integration Automation cell, showing the 4LAB™, 4s3™ and De-Framer fully integrated

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