Tijdschrift voor Entomologie 161 (2018) 111–130 The genus Lambulosia Hampson, 1914 from New Guinea (Lepidoptera: Erebidae, Arctiinae, Lithosiini) Rob de Vos The genus Lambulosia Hampson, 1914 (Lepidoptera: Erebidae, Arctiinae, Lithosiini) is revised. The genus is restricted to New Guinea. Nine new species were discovered to belong to this originally monotypic genus. Two species groups are recognized, the aurantiaca species group with six species, of which five are new to science: Lambulosia aurantiaca (Rothschild, 1912), L. arabuensis sp. n., L. gemmata sp. n., L. metallescens sp. n., L. flavoradiata sp. n. and L. brunnescens sp. n.; and the nigrolineata species group with four species new to science: L. nigrolineata sp. n., L. nigrotriangula sp. n., L. obscurata sp. n. and L. punctilineata sp. n. All species are described and the adults and genitalia are depicted. Rob de Vos, Naturalis Biodiversity Center, Darwinweg 2, 2333 CR Leiden, The Netherlands. [email protected] Introduction Materials and methods The Arctiinae (Erebidae) in the Indo-Australian re- gion are known for the many genera with only one Material or very few species, and on the other hand for a few For this revision 44 specimens were examined in the very large genera with numerous species. The last following collections: decades many genera have been revised by several authors and this revealed that many sibling species KSP Koleksi Serangga Papua, Waena, Papua, were hidden in what was considered a well known Indonesia (former collection of Henk species or species group (Holloway et al. 2001). The van Mastrigt) same goes for the genus Lambulosia Hampson, 1914, NHMUK (in some specimen numbers referred to which was originally described as monotypic with as BMNH(E)) - Natural History Mu- type species Lambula aurantiaca Rothschild, 1912. seum, London, United Kingdom Recently nine new species were discovered to belong RMNH (in specimen numbers referred to as to this genus, all restricted to New Guinea and found RMNH.INS.) - Naturalis Biodiversity at high altitudes. The ten known species of this ge- Center, Leiden, The Netherlands (for- nus can be arranged into two species groups, with six merly Rijksmuseum van Natuurlijke and four species, respectively, judging from the strik- Historie) ing differences in their genitalia. The identical struc- ture of the wings, colour pattern and abdomen, with Methods some characteristic features, is the reason to consider The genitalia of all species studied (at least one male both groups as belonging to one genus. One species and one female specimen per species) were dissected, is found in Papua New Guinea, the other nine were mounted and depicted when available. Dissected found in the Indonesian part of the island. genitalia were put in cold KOH 10% for one night. Tijdschrift voor Entomologie 161: 111–130, Figs 1–45. [ISSN 0040-7496]. brill.com/tve © Nederlandse Entomologische Vereniging. Published by Koninklijke Brill NV, Leiden. Published 16 September 2019. DOI 10.1163/22119434-20192079 Downloaded from Brill.com09/24/2021 08:16:37PM via free access 204346 112 Tijdschrift voor Entomologie, volume 161, 2018 After washing and cleaning with 30% alcohol to Lambulosia nigrotriangula sp. n. remove fat, scales and dirt, the genitalia were stained Lambulosia obscurata sp. n. in a solution of chlorazol black in 30% alcohol and Lambulosia punctilineata sp. n. fixed in 95% alcohol. Then the genitalia were pre- pared for preservation in Euparal Essence (to prevent air bubbles) and after a few minutes mounted on a Keys to the species of the genus glass slide in a few drops of Euparal medium follow- Lambulosia ing the standard used in the Natural History Mu- seum in London (Robinson 1976): abdomen on the Wing pattern left side, genital armature top right, aedeagus bot- tom right. To prevent the smaller parts, like the ae- 1. Forewing with ground colour yellow ............ 2 deagus, from floating the Euparal drops were left to – Forewing with ground colour grey or brown .. dry at least 24 hours without glass cover in a closed .................................................................... 6 Petri dish (to protect against dust). The next day a 2. Forewing without transverse lines ................ 3 proper size glass cover was put on the parts in the – Forewing with transverse lines ...................... 5 sticky Euparal, adding a few fresh drops of Euparal. 3. Forewing with a broad dark margin along ter- The complete slide was left to dry for weeks to men and costa, continuing as a metallic blue months in the Petri dish. Labels were only added af- subbasal triangular band ...................gemmata ter drying completely, so therefore it was necessary – No such broad dark margin on the forewing .. to write slide number and details on the petri-dish .................................................................... 4 cover and to scratch the number on the glass slide 4. Forewing with a dark subbasal costal spot, to avoid mixing up with other drying slides. All hindwing plain-coloured ...........nigrotriangula slides were made by the author and were numbered – Forewing costa subbasally at most with a dark with standard numbers of the labels provided by the rim, hindwing at margin with dark suffusion museum. .......................................nigrolineata (female) Slides were studied with a WILD M3 binocular 5. Transverse lines sharp and black, finely den- microscope at 60–400× magnification. Digital pho- tated ................................. nigrolineata (male) tographs were made with a motorized Zeiss V20 bin- – Transverse lines rather diffuse, space in be- ocular microscope and a digital Axio MRc5 camera tween can be filled by dark colour forming a controlled by Axiomanager M2 software. broad fascia ....................................aurantiaca Forewing lengths (Fwl) were measured with a digi- 6. Forewing with costal spots or a fascia ........... 7 tal calliper from forewing base to apex; numbers were – Forewing without a trace of spots or a fascia .. rounded to 0.5 mm. Morphological terminology of .................................................................... 9 the external structures (excluding the genitalia) main- 7. Brown forewing with a slightly darker fascia ly follows Scoble (1992) and Holloway et al. (2001). and diffuse transverse lines .............arabuensis The terminology of the genitalia mainly follows – Forewing with two costal spots (connected to Tuxen (1970) and Kôda (1987). transverse lines) ............................................ 8 8. Hindwing dark grey, forewing with transverse Results lines consisting of dots ................punctilineata – Hindwing cream-white or pale yellow, trans- verse lines on forewing unbroken ..... Checklist Lambulosia species obscurata 9. Forewing with yellow longitudinal stripes LAMBULOSIA Hampson, 1914 along the veins ............................. Type species: Lambula aurantiaca Rothschild, 1912 flavoradiata – Forewing plain-coloured, basally with blue The aurantiaca species group metallic shimmer ....................................... 10 Lambulosia aurantiaca (Rothschild, 1912) 10. Forewing dark brown, basally with some faint Lambulosia mediosuffusa Strand, 1922 blue metallic shimmer ...................brunnescens Lambulosia pallicolor Strand, 1922 – Forewing grey brown, basally with distinct Lambulosia arabuensis sp. n. blue metallic shimmer ...................metallescens Lambulosia gemmata sp. n. Lambulosia brunnescens sp. n. Genitalia Lambulosia metallescens sp. n. Lambulosia flavoradiata sp. n. 1. Male genitalia ................................................ ............... 2 (male of flavoradiata is unknown) The nigrolineata species group – Female genitalia ....................... 10 (females of Lambulosia nigrolineata sp. n. arabuensis, brunnescens and obscurata are unknown) Downloaded from Brill.com09/24/2021 08:16:37PM via free access 204346 Rob de Vos: The genus Lambulosia Hampson 113 2. Cucullus and sacculus separated into slender 14. Bursa copulatrix at left and right side longitudinal parts without any processes, ae- strongly covered with large thorn-like signa deagus long and slender (aurantiaca species ...................................................punctilineata group ........................................................... 3 – Bursa copulatrix at least the distal half entirely – Cucullus and sacculus at base connected, cu- sclerotized .................................................. 15 cullus with one or more additional processes, 15. Bursa copulatrix almost entirely sclerotized, aedeagus short and robust (nigrolineata species caudally scobinated with numerous tiny group) .......................................................... 7 spines which are arranged and clustered in 3. Extension of cucullus with blunt apex, apex irregular curved ridges .................................... of straight sacculus with a long sharp thorn nigrolineata .......................................................arabuensis – Caudal half of bursa copulatrix scobinated – Extension of cucullus with sharp apex .......... 4 with regularly divided tiny spines ................... 4. Extension of cucullus with a hockey-stick- .................................................nigrotriangula shaped apex, sacculus strongly curved and with a spatula-shaped apex ............metallescens – Extension of cucullus not with a hockey-stick- The aurantiaca species group shaped apex, apex of sacculus sharp .............. 5 General characters: Antennae in male and female fi-