NJ Department of Environmental Protection Water Monitoring and Standards

AMBIENT BIOMONITORING NETWORK

Raritan Water Region

Watershed Management Areas 7, 8, 9, and 10 Round 3 Benthic Macroinvertebrate Data Volume 1 of 2

February 2008

State of NJ Department of Environmental Protection Jon S. Corzine, Governor Lisa Jackson, Commissioner

NJ Department of Environmental Protection Land Use Management Mark Mauriello, Assistant Commissioner

Water Monitoring and Standards Leslie McGeorge, Administrator

Bureau of Freshwater & Biological Monitoring Alfred L. Korndoerfer, Jr., Chief

February 2008

AMBIENT BIOMONITORING NETWORK

Raritan Water Region Watershed Management Areas 7, 8, 9, and 10

Round 3 Benthic Macroinvertebrate Data

Volume 1 of 2

Water Monitoring Report Prepared By: Bureau of Freshwater and Biological Monitoring

Sampling and Data Analysis: Victor Poretti, Project Manager-Sampling Coordination Dean Bryson, Project Manager-Laboratory Operations Jessica Messersmith Thomas Miller Anna Signor

Report Preparation: Thomas Miller

Map Preparation: John Sell

Edited By: Alfred Korndoerfer Leslie McGeorge Alena Baldwin-Brown

AMBIENT BIOMONITORING NETWORK Watershed Management Areas 7, 8, 9, and 10

Raritan Water Region

Round 3 Benthic Macroinvertebrate Data

Volume 1 of 2

TABLE OF CONTENTS

page Executive Summary 1 Introduction 3 Rationale for Biological Monitoring 3 Advantages of Using Benthic Macroinvertebrates 3 Limitations of Biological Monitoring 3 Benthic Macroinvertebrates Usually Indicative of Good Water Quality 4 Benthic Macroinvertebrates Usually Indicative of Poor Water Quality 5 Study Design 6 Data Quality Objectives 6 Site Selection 6 Field and Laboratory Methods 7 Sample Collection 7 Sample Processing and Sorting 8 Macroinvertebrate Identification and Quality Control 8 Data Analysis 8 Trend Analysis 9 Supplemental Analyses/Evaluation Methods 9 Morphological Abnormalities 9 Habitat Assessment 10 Fish IBI 10 Chemical Monitoring 11 Results and Discussion 13 Summary of Statewide AMNET Data 13 Results & Trends 14 Regional Results 15 Evaluation by WMA 16

i Watershed Management Area # 7 16 Watershed Management Area # 8 17 Watershed Management Area # 9 18 Watershed Management Area # 10 19 Macroinvertebrate Abnormalities 20 Causes and Conditions of Impairment Condition 20 Habitat Assessment vs. Biological 21 Additional Information 22 REFERENCES 23 TABLE 1. Biological Criteria for Screening Water Quality in New Jersey Freshwater Streams

ii Ambient Biomonitoring Network Watershed Management Areas 7, 8, 9, and 10

Raritan River Region

Round 3 Benthic Macroinvertebrate Data

Volume 1 of 2

EXECUTIVE SUMMARY

Biological monitoring of freshwater systems in New Jersey provides an effective means of gauging long-term trends in surface water quality throughout the State. The Ambient Biomonitoring Network (AMNET) is one of the major ongoing monitoring programs. This statewide network of over 800 AMNET stations employs sampling and taxonomic analysis of in-stream macroinvertebrate communities to assess the ecological condition at each station. Following sample analysis, an integrated index of "biometrics", based on community composition and pollution tolerance levels of individual taxa, assigns one of three "impairment" levels to each site (i.e., non-impaired, moderately impaired, or severely impaired). The results are considered reflective of the water or habitat quality at each site. This information is used by the Department, primarily in assessing progress toward the goals of the Clean Water Act via the Integrated Water Quality Monitoring and Assessment Report. AMNET data are also very useful for designation of Category 1 waters based on exceptional ecological significance. Results are reported separately for each of New Jersey’s five major drainage basins or “Water Regions” (Lower Delaware, Upper Delaware/Northwest, Northeast, Raritan and Atlantic), each encompassing several sub-basins (“Watershed Management Areas”). The Water Regions, with an average of 165 AMNET sites each, are sampled in consecutive years on a five-year rotational basis.

This report presents the results for the biological 2004 Raritan Region monitoring conducted in Raritan Water Region from Bioassessment Results April to October 2004. The sampling of this Region Non-impaired (160 total sites) 33.8% marks the third round of data collection for this basin. For the Raritan Water Region, the results obtained in Moderately the current round are similar to those of the previous impaired (second round) sampling. Currently, of 165 AMNET 63.7% sites in the Raritan Water Region, 54 (33.75%) were found non-impaired, 102 (63.75%) moderately Severely impaired impaired, and 4 (2.5%) severely impaired, with five 2.5% sites not sampled (AN0202, AN0382D, AN0389, AN0454, AN0455) due to site difficulties or bridge construction.

Results from the current, 3rd round (2004) of sampling are compared to those from the same sites sampled in the earlier rounds (1994, 1999). Of the 165 total AMNET sites presently in the Raritan Water Region, current sampling yielded substantially fewer severely impaired sites (2.5%) than did the first and second round sampling (5.6%, 9.2% respectively ); however, the curent round yielded more moderately impaired sites (63.75%) than did the first and second rounds (56.9%, 55.6% respectively ). Conversely, the number of non-impaired sites (33.75%) observed in the 2004 sampling Page 1 of 23 continued the decline consistently observed since the first and second rounds of sampling (37.5%, 35.2% respectively). Percent Change in Rating Between the As reflected in the present study results, human 1999 and the 2004 Monitoring land uses and practices, superimposed on the (160 sites total) undisturbed physical terrain, play a major role in controlling the degree of pollution or degradation Positive Change in a stream system. The relationship between 12.5% benthic macroinvertebrate community impairment No Change 71.9% has been statistically related to different Negative Change physiographic land types, land uses and other 15.6% anthropogenic factors, on a statewide basis. These findings strongly indicate that human land uses and practices play a major role in the degree of pollution or degradation in a stream system. [15]

To determine what factors are contributing to impairments, or changes in impairment ratings, the Department has established a Stressor Identification (SI) process. The purpose of the Stressor Identification (SI) process, as developed by USEPA, is to identify the principle stressor(s), including but not limited to, specific pollutants responsible for the degraded biological condition. Identifying whether the principal stressor(s) is a pollutant or, if a specific pollutant(s) cannot be identified, is due to generic pollution is the first step towards deciding whether a pollutant(s) specific TMDL or other appropriate management measures will be taken to remediate the impairment. Five sites have been selected in this Water Region for initial Stressor Identification work. These sites are: AN0311 (Drakes Brook), AN0324 (Beaver Brook), AN0343 (Holland Brook) and AN0333 & AN0337 (Neshanic River).

Page 2 of 23 INTRODUCTION

Rationale for Biological Monitoring Biological monitoring, as referenced in this report, pertains to the collection and analysis of stream macroinvertebrate communities as indicators of water or habitat quality. Macroinvertebrates are larger- than-microscopic, primarily benthic (bottom-dwelling) fauna, which are generally ubiquitous in freshwater and estuarine environments, and play an integral role in the aquatic food web. Insects (largely immature forms) are especially characteristic of freshwaters; other major groups include worms, mollusks (snails, clams) and crustaceans (scuds, shrimp, crayfish, etc.). They are more readily collected and quantified than either fish or periphyton communities. Species comprising the in-stream community occupy various niches, based on functional adaptation or feeding mode (e.g., predators, filter or detritus feeders, scavengers); their presence and relative abundance is governed by environmental conditions (which may determine available food supply) and by pollution tolerance levels of the respective taxa. The overall community, thus, is holistically reflective of conditions in its environment. Assessments of ambient water / habitat quality can then be made based upon standardized procedures, which can show perturbations measured as changes or differences in community structure [1]. While development of a "multitrophic" approach to include finfish and periphyton communities with invertebrates is being investigated, the primary means of assessment to date has been through macroinvertebrate community analysis.

Advantages of Using Benthic Macroinvertebrates:

1. They are good indicators of localized conditions of water quality due to their limited mobility. As such, they are well suited for the assessment of site-specific pollution impacts. 2. They are sensitive to environmental impacts from both point and non-point sources of pollution. 3. They integrate the effects of short-term environmental variations, such as oil spills and intermittent discharges. 4. Sampling is relatively easy and inexpensive. 5. They are holistic indicators of overall water quality, even for substances that may be present, but at lower than detectable levels. 6. They are normally abundant in New Jersey waters as well as aquatic environments in general. 7. They serve as the primary food source for many species of commercially and recreationally important fishes. 8. Unlike chemical monitoring, where impacts to the environment tend to be by inference, not direct determination, they provide a direct measure of water quality in a manner consistent with the goals of the Clean Water Act. 9. They can be used to assess nonchemical impacts to the aquatic habitat, such as by thermal pollution, excessive sediment loading (siltation), or eutrophication. 10. To the general public, impacts to resident benthic macroinvertebrate communities are more tangible measurements of water quality than more esoteric listings of chemical test results. 11. When monitored together with relevant chemical/physical parameters, benthic macroinvertebrate communities can be used to identify sources of impairment.

Limitations of Biological Monitoring:

Biological monitoring cannot replace chemical monitoring, toxicity testing, and other standard environmental measurements. Each of these tools provides the analyst with specific information available only through its respective methodology.

Page 3 of 23 The following illustrations provide an overview of the major macroinvertebrate indicator groups employed in making biological water quality assessments.

Benthic Macroinvertebrates Usually Indicative of Good Water Quality

Mayfly nymphs are often abundant wherever the water is clean. They are sensitive to various types of water pollution, including low dissolved oxygen, ammonia, biocides, and metals.

Stonefly nymphs are usually found only in cool, well-oxygenated waters free of pollution. Though not usually found in the numbers characteristic of mayflies, the presence of even a few stoneflies is indicative of good water quality.

Most caddisfly larvae, many of which build portable cases of stones, sticks, sand, and other detritus, are intolerant of water pollution.

Aquatic beetles are common in well-oxygenated, swiftly running waters; many species are referred to as “riffle beetles.” They are usually indicative of clean water since they are sensitive to wetting agents (soaps and detergents) and other pollutants.

All photographs taken by D.Bryson, NJDEP

Page 4 of 23 Benthic Macroinvertebrates Usually Indicative of Poor Water Quality

Midges (chironomids) are among the most common of aquatic invertebrates. They occupy a variety of aquatic habitats, including lakes, ponds, bogs, rivers, creeks, and marshes. They even exploit manmade habitats such as sewage treatment plants, water treatment plants, fish pools, irrigation ditches, and birdbaths. Many species are very tolerant of pollution.

Aquatic sowbugs, or freshwater isopods, are abundant in waters enriched with organic nutrients and low in dissolved oxygen. They are commonly observed in the recovery areas below sewage treatment plants.

Leeches and other segmented worms are very common in our lakes and streams, though not often noticed. They are tolerant of poor water quality and severe pollution.

Black fly larvae are filter feeders, capturing and ingesting plankton and bacteria from the surrounding water with specialized antennae. Some species are very tolerant of poor water quality and thus can be used as indicators of pollution.

All photographs taken by D.Bryson, NJDEP

Page 5 of 23 STUDY DESIGN

Data Quality Objectives The major goal of AMNET is to provide a long-term, cost-efficient means of gauging the quality of surface waters and watershed areas throughout the State. This is accomplished through biological sampling and analysis from a network of stream sites that adequately represents New Jersey’s five major drainage basins and NJDEP’s Watershed Management Areas (WMA). Administratively, a total of twenty-one WMA's have been delineated within New Jersey's five basins. Each major basin constitutes a "Water Region”; a major sub-basin forms each WMA. Within each WMA are several smaller sub-basins, delineated by the United States Geological Survey (USGS) as “hydrologic units," scale 11 (HUC11). The study area of the present report includes WMA #’s 7 (Elizabeth, Rahway, Woodbridge), 8 (North and South Branch Raritan), 9 (Lower Raritan, South River, Lawrence Brook), and 10 () (see Maps 1 – 7, Volume 2). The standard sampling interval of five years reflects a realistic temporal lag between cessation of an environmental perturbation and recovery of the impacted biological community. The Integrated Water Quality Monitoring and Assessment Report [2], which re-examines changes in New Jersey’s stream systems on a two-year cycle, has indicated that five years is an optimum period for long-term biomonitoring. An ample network of stations is required for the creation of a long-term database, which in turn, is necessary for trend analysis and operation of water quality predictive models.

The AMNET program is designed to monitor a complete Water Region's complement of stations within a year's time, giving modelers and planners a snapshot of ambient biological impacts during that particular year. Sampling is curtailed through the coldest months (December to March) because of difficulties encountered in obtaining representative samples during this period.

SITE SELECTION

Sites were selected essentially to provide representative coverage of each Water Region, as well as the entire State. To ensure enough flow for sampling, sites on "first-order" streams are situated at least three miles downstream of headwaters (first order streams are those with no tributaries). Since most streams at this level have very little (or only intermittent) flow, most of the sites are situated on second-order streams (with only first-order streams as tributaries) and higher (with a greater hierarchy of tributaries). All sites are located in reasonably accessible and primarily wadable segments, proceeding downstream to the head- of-tide. Sites are numbered in approximate upstream to downstream order, from the mainstem of each major sub-basin to each adjacent tributary, and then to the next adjacent sub-basin. This is in an approximate north to south order within the Raritan Water Region.

To maximize data correlation, AMNET, wherever possible, incorporates existing stations of the Ambient Surface Water Chemical Monitoring Network, which is administered jointly by NJDEP and the USGS [3]. Furthermore, so as to gauge the effects of major tributaries and larger lakes, many AMNET sites are located near their confluence or outlet. An attempt is made when selecting sites to obtain a sample representative of the stream’s total water quality. Sites are placed down stream of features such as known sources of contamination (e.g., point-source discharges, agricultural operations), or significant natural features such as wetlands, parks or wildlife management areas when it is determined that these features have a dominant impact (positively or negatively) on the stream.

Exact AMNET site locations were determined via the Global Positioning System (GPS) using Trimble Pathfinder units and the appropriate correction sources utilized by NJDEP. All positions were logged into the DEP’s Geographical Information System (GIS) (see Maps 1 – 7, Appendix A, Volume 2). Page 6 of 23 For the first round of AMNET, a total of 146 stations had been established in the Raritan basin including the North and South Branches of the , Millstone and South Rivers, and smaller sub-basins [4].

A total of 165 sites were established for the previous round of AMNET sampling in the Raritan Water Region (1999) [5]. This area (shown in Figure 1) primarily includes all New Jersey sub-basins draining to the Raritan River (WMA's #7, 8, 9, and 10). For the second round of AMNET (1999), the original study area was realigned to conform with the boundary between the newly established Water Regions [5]. Those sub-basins that drain to Raritan Bay via the Arthur Kill, i.e., the Elizabeth, Rahway, and Woodbridge rivers (now WMA 7), which were formerly part of the greater Passaic (Northeast) basin [6], have been included in the present study. The present Raritan study area (Figure 1) includes a total of 165 sampling sites, AN0194 – 204, AN0310 – 455 (see Table 2, Volume 2). Sites AN0202 (WB Elizabeth River), AN0382D (Millstone River), AN0389 (Devils Bk), AN0454 (Deep Run), and AN0455 (Tennent Bk) in the current data set were either too deep, had difficult access or there was bridge construction when visited and we were unable to sample these sites. Sites AN0454 and AN0455 also weren’t Figure 1 sampled during the first round of sampling [4]. Map of 2004 study area

FIELD & LABORATORY METHODS

Benthic macroinvertebrate sampling and analysis is performed in accordance with the NJDEP Field Procedures Manual [7], Rapid Bioassessment Protocol (RBP) guidelines of the USEPA [8] and Standard Operating Procedures (SOP) of the NJDEP Aquatic Biomonitoring Laboratory [9]. As detailed in the SOP and in the quality assurance work plan [10], a thorough quality control program, with emphasis on macroinvertebrate taxonomy, is practiced.

Sample Collection In general, a "multi-habitat" approach is used, focussing on the more productive habitat types [8]. The usual sampling device is a D-frame kick net of 800 x 900 um mesh size and one foot width (a Surber sampler or Ponar dredge may be employed when conditions require). In high-gradient streams, where the predominant substrate is cobble, the riffle/run area is the preferred sampling habitat; other likely habitat types are sampled when present. The kick net is held firmly against the hard bottom, and an area approximately one foot upstream of the net is disturbed using feet and/or hands. This procedure is repeated, sampling all velocity/depth regimes at the site, including at least one riffle-run-riffle sequence (if present). In the low-gradient Coastal Plain streams, bottoms generally consist of sand or mud without dominant cobble/riffle areas; therefore, a variety of stable substrates including woody debris, submerged macrophytes and portions of banks are sampled. The "jab and sweep" method [11] is employed; a minimum of 20 jabs/sweeps are taken, proportioned approximately to the numbers of each habitat type present. In all cases, stream distance sampled approaches, but does not exceed, 100M. Level of effort is consistent for all sites. In the presence of road crossings, where possible, sampling is done upstream of bridges, sufficiently removed to avoid influence of any associated channel alterations. The entire sample is sieved using a #30 mesh sieve bucket, put into wide-mouthed (1-L) jars, and preserved with 5 to 10% formalin (to 20% in cases of excessive organic loading). Both the sieve bucket and net are examined for adhering organisms. Any found are removed with forceps and placed into the sample jar. During the field operations, qualitative observations of habitat, surrounding land use, potential pollution sources, and presence of other aquatic biota are recorded (Appendix D, Volume 2); a visual-based qualitative habitat assessment [8] is also performed (see p. 10). These observations/evaluations, however, do not factor into Page 7 of 23 the final bioassessment rating.

Sample Processing and Sorting In the laboratory, after rinsing in a #30 mesh sieve to remove the preservative, the composited sample is evenly distributed in a light-colored pan marked with grids of equal size. Using low-power magnification (6.3x), all organisms greater than 2mm in size are then removed from each randomly selected grid until a total of at least 100 organisms is obtained. Colonial groups (e.g., Bryozoa and Porifera), vertebrates, and terrestrial organisms are not included in the subsample. Organisms retained are reasonably intact to allow for accurate identification.

Macroinvertebrate Identification and Quality Control The individuals from the subsample are identified to the lowest practicable taxonomic level, usually genus or species, using 7 to 30X stereozoom and 40 to 400X compound magnification. The Biomonitoring Unit currently uses Leica Model MZ6 stereomicroscopes and Leica Models DMLS and DME compound microscopes. A computerized digital camera system projects and records microscope images of selected specimens to aid in their identification. A comprehensive collection of taxonomic keys and other references, including functional (or niche) descriptions and pollution tolerance classifications for most species, is maintained in the laboratory. An indexed list of these is given in the Laboratory SOP [9]. Pertinent new reference material is added when available. Taxonomists confer with each other regarding species in question. The International Taxonomic Information System (ITIS) (www.itis.usda.gov) is monitored for possible changes in nomenclature or groupings. Consultation with other scientists in the field, particularly from agencies involved in similar programs (e.g., New York Department of Environmental Conservation, USGS, USEPA), provides added assistance and confirmation when needed. For verification, 10% of the samples are sent to a qualified independent consultant for parallel identifications. A macroinvertebrate specimen reference collection is maintained in the laboratory.

Data Analysis Biological impairment may be caused by several major factors such as organic enrichment, habitat degradation, or toxicological effects. It may be manifested in several aspects of the benthic macroinvertebrate community. These include: absence of pollution-sensitive taxa, especially the EPT group (i.e., Ephemeroptera (mayflies), Plecoptera (stoneflies) and Trichoptera (caddisflies)); excessive dominance of pollution-tolerant taxa such as Chironomidae (midges) and Oligochaeta (worms); low overall taxa numbers; or other perceptible differences in community structure relative to a reference condition.

The data analysis is an important part of the RBP protocol, developed under USEPA auspices as an expedient and cost-effective monitoring tool. It recognizes the use of community metrics and the pollution indicator concept. “Biometrics” measure different components of community structure, including population and functional parameters, each with a different range of sensitivity to pollution stresses [1, 12]. The use of a variety of biometrics assures a more robust or valid assessment; therefore, an anomaly in any one metric is less likely to invalidate the study findings. The results are integrated through common scoring criteria, derived from an established comparable database, to determine a final numerical rating and consequent biological condition category (see Table 1). This provides the analyst with an easily communicated evaluation of relative impairment, referred to in this report as the “bioassessment rating." For RBP protocols, results are based on 100 organism sub-samples. Scoring criteria for RBP protocols [1] are calibrated for family level taxonomy, giving three final rating categories (non-impaired, moderately impaired, and severely impaired). Page 8 of 23

The biometrics employed, and subsequent integrated index, were developed as outlined in RBP methods [1]. The final numerical rating is referred to as the “New Jersey Impairment Score” (NJIS) and was statistically validated based upon data from 200 New Jersey stream sites [13]. The scoring criteria and rating categories are presented in Table 1. The metrics from which the NJIS is derived are explained below:

1. Total Taxa or Taxa Richness (# families) — an index of community diversity; the number usually increases with increasing water or habitat quality.

2. Percent Contribution of the Dominant Family (to the total # families) — dominance by relatively few species/families would indicate environmental stress.

3. # EPT Families — the number of families represented within the orders Ephemeroptera (mayflies), Plecoptera (stoneflies) and Trichoptera (caddisflies), which are generally pollution-sensitive.

4. Percent EPT (of the total # individuals) — would increase with increasing water quality.

5. Hilsenhoff (Family) Biotic Index — tolerance values of 0 - 10 are assigned to individual families (zero = most intolerant); these values are used in the formula for calculating the Biotic Index which summarizes the overall pollution tolerance of the entire benthic macroinvertebrate community with a single value.

Trend Analysis In evaluating the current AMNET data against that for the previous round, a significant improvement or decline is considered to have occurred if the difference in NJIS scores has changed the bioassessment rating. A complete list of site-by-site comparisons is presented in Table 2, where a (+) indicates a significant improvement, a (—) indicates a significant decline, and a (/) indicates no change in rating; a slash may have a (+) or a (-) indicating that the score improved or declined, but the bioassessment rating did not. If a site was only sampled once in concurrent rounds, the change will have "nd" meaning there was "no data" available for a comparison.

SUPPLEMENTAL ANALYSES / EVALUATION METHODS

Morphological Abnormalities Occasionally, morphological abnormalities have been found in individual macroinvertebrates recovered in the Bureau of Freshwater & Biological Monitoring’s (BFBM) AMNET collections. These deformities have been most readily detected in the Chironomidae (midges), where they occur primarily in the head appendages (antennae) and mouth parts (mentum and mandibles). While the incidence has been most frequent in the chironomids (especially those species categorized as detritivores, herbivores or periphyton feeders), abnormalities have also been observed in individuals of other taxonomic groups. Although this is not a factor in the NJIS data analysis, such features are noted as they may signify possible contaminants or stressful conditions in the respective drainages.

Abnormalities observed in the course of identification are noted; these results are summarized by sample site in Table 3, Volume 2. For Chironomidae, the data are displayed as # of chironomids with abnormalities / # of chironomids examined. For all other taxa, just the number of individuals with abnormalities is presented. Photographic examples of abnormalities in midge larvae and amphipods Page 9 of 23 (scuds) are presented in Appendix B, Volume 2.

Habitat Assessment The physical attributes of habitat play an integral role in the health of the macroinvertebrate community. Where stations are physically comparable, detected impacts can be attributed to water quality factors; however, physical habitat degradation alone can account for biological impairment in a stream [1]. Parameters evaluated include in-stream substrate, channel morphology, bank structural features, and riparian vegetation. The area evaluated includes the sample site and its immediate surroundings, particularly upstream, usually within a 100 – 200 foot radius. The visual-based qualitative habitat assessment results in one of four condition categories: optimal, suboptimal, marginal or poor, as outlined in the 1999 revisions to the USEPA criteria [8].

The habitat assessment is separated into two basic approaches; one designed for high gradient streams and one designed for low gradient streams [8]. Examples of assessment forms for each approach can be found in Appendix C, Volume 2. Streams in the northern regions of New Jersey are generally considered to be “high gradient” streams, having substrates of rock and cobble of various sizes, and with relatively swift flow. Those in the Coastal Plain regions of southern New Jersey are considered as “low gradient” streams, having slower flow and more homogeneous substrates, primarily of sand or gravel and finer sediments. Habitat assessments may be temporarily downgraded by adverse weather conditions, such as excessive rainfall or prolonged drought. It should also be noted that habitat assessments are performed independently of the macroinvertebrate community analysis; thus, they do not factor into the final impairment score, but are used primarily as supplementary information.

Fish IBI In addition to the AMNET sampling performed on freshwater streams, BFBM also supplements the benthic monitoring by performing a fish Index of Biotic Integrity (IBI) analysis at or near many AMNET stations. An IBI is an index that measures the health of a stream based on multiple attributes of the resident fish assemblage. Each site sampled is scored based on its deviation from reference conditions (i.e., what would be found in an unimpacted stream) and classified as poor, fair, good or excellent.

Data provided by the IBI have become another component of the NJDEP's suite of environmental indicators. The data help to measure water quality use attainment and the Department's success in attaining the Clean Water Act goal of "fishable" waters as elaborated in the Department's Integrated Water Quality Monitoring and Assessment Report. IBI data will also be used to develop biological criteria, prioritize sites for further studies, provide biological impact assessments, and assess status and trends of the state's freshwater fish assemblages.

There were 21 IBI sites performed in the Raritan basin prior to this round of sampling. Of them, 1 was Poor, 9 were Fair, 9 were Good and 2 displayed an Excellent IBI Rating. For more info on the Fish IBI results and reports, visit the BFBM web site at: www.state.nj.us/dep/wms/bfbm.

Page 10 of 23 Chemical Monitoring The Bureau of Water Quality Standards and Assessment (BWQSA) is responsible for the development, adoption, and administration of New Jersey's Surface Quality Standards (SWQS) and Ground Water Quality Standards (GWQS) [14]. This includes the development of water quality criteria to protect aquatic life and human health, the assignment of stream classifications to reflect existing and designated uses, and the promulgation of antidegradation policies to protect and maintain the quality of surface and ground waters of the State. The SWQS are used by many DEP programs including: the New Jersey Pollutant Discharge Elimination System program, Site Remediation program, Stream Encroachment and the Land Use Regulation Program.

The SWQS form the basis for monitoring the degree of impairment of surface water bodies and for calculating total maximum daily loads (TMDLs), which represent the assimilative capacity of surface water for a given parameter of concern. The development of TMDLs includes balancing the impacts from point sources, non-point sources and natural background conditions. TMDLs are developed on a watershed basis to aid watershed management planning efforts. The State develops a list of impaired waters (303d) and a surface water quality inventory report (305b) every two years.

BWQSA is also responsible for conducting and coordinating water quality assessments of all waters of the State. These assessments are reported through the New Jersey Integrated Water Quality Monitoring and Assessment Report (Integrated Report). [2] Historically, the Department summarized statewide water quality in a biennial report entitled, "New Jersey's Water Quality Inventory Report" (also known as the "305(b) Report") and proposed a separate "303(d) list" or "List of Impaired Waters". The current USEPA format for these reports (instituted in 2002) integrates the reporting requirements of Sections 303(d) and 305(b) of the Federal Clean Water Act into one comprehensive, integrated water quality assessment report.

To prepare the Integrated Report, BWQSA compiles available monitoring data from various agencies and organizations that collect measurements from the State’s streams. The physical/chemical data is compared to water quality criteria outlined in the SWQS. Values for each measured parameter are evaluated and used to determine whether the waterway is in “full attainment of aquatic life use” or in “non-attainment of aquatic life use” based upon the levels outlined in those standards.

In this report, AMNET results were compared to BWQSA’s use attainment designations as assessed using physical / chemical specific criterion. A list of the AMNET sites corresponding to stream segments designated by BWQSA between 2004 and 2006 as “non-attainment of aquatic life use” based upon physical/chemical criterion can be found in Table 5, Volume 2. Eighty-four (84) sites were designated as “non-attainment of aquatic life use” for at lease one physical / chemical parameter; the most common exceedences of the Surface and Ground Water Quality Standards being Temperature and Total Phosphorus. Of these 84 sites, 51 sites were designated as “non-attainment of aquatic life use” for two or more of the following physical / chemical parameters: Total Phosphorus, Total Dissolved Solids, Total Suspended Solids, pH, Dissolved Oxygen, and Temperature. All but twenty- five (25) AMNET sites, where an exceedence occurred, received a Moderately or Severely Impaired biological assessment. The remaining twenty-five (25) AMNET sites where an exceedence occurred received a Nonimpaired biological assessment (see Table 5, Volume 2). Special attention should be given to the nonimpaired sites that exceeded standards. Continued degradation of the water quality will likely downgrade the nonimpaired assessment in the future.

Page 11 of 23 The Department will attempt to identify the potential sources of impairment using the Department’s Stressor Identification (SI) process. The purpose of the Stressor Identification (SI) process is to identify the principle stressor(s), including but not limited to specific pollutants, responsible for the degraded biological condition. . Identifying whether the principal stressor(s) is a pollutant* or due to more generic landscape changes caused by human activities, is the first step towards deciding whether a pollutant(s) specific TMDL or other appropriate management measures will be taken to remediate the impairment. Five sites have been selected in this Water Region for initial Stressor Identification work. These sites are: AN0311 (Drakes Brook), AN0324 (Beaver Brook), AN0343 (Holland Brook) and AN0333 & AN0337 (Neshanic River).

* As defined in the N.J. Water Pollution Control Act (N.J.S.A. 58:10A-1 et seq.) and the Federal Water Pollution Control Act, aka “Clean Water Act” (33 U.S.C. 1251-1376)

Page 12 of 23 RESULTS AND DISCUSSION

Summary of Statewide AMNET Data

The current study marks the third round of sampling for the Raritan basin AMNET study. Of the regions sampled to date for the third round, the Upper Delaware continues to yield considerably more nonimpaired sites than did the second round results for all other New Jersey Water regions. The Raritan basin has not shown any considerable changes since the previous rounds but the percent of nonimpaired sites has shown a slight decline. The table below presents the proportions of non-impaired, moderately and severely impaired AMNET sites for all New Jersey Water Regions in the first and second AMNET round, plus the third round for the Raritan, Northeast and Upper Delaware/Northwest Water Region.

Region Number of sites

Third round Non-impaired Moderately Severely Total sites impaired impaired Upper Delaware 78 (54.9%) 60 (42.3%) 4 (2.8%) 142 Northeast 32 (31.1%) 68 (66.0%) 3 (2.9%) 103 Raritan 54 (33.8%) 102 (63.7%) 4 (2.5%) 160 Second round Upper Delaware 80 (58.0%) 57 (41.3%) 1 (0.7%) 139 Northeast 38 (36.9%) 59 (57.3%) 6 (5.8%) 103 Raritan 57 (35.2%) 90 (55.6%) 15 (9.2%) 162 Atlantic 75 (35.2%) 115 (54.0%) 23 (10.8%) 213 Lower Delaware 31 (15.7%) 139 (70.6%) 27 (13.7%) 197 First round Upper Delaware 87 (45.8%) 71 (37.4%) 32 (16.8%) 190 Northeast 36 (31.3%) 63 (54.8%) 16 (13.9%) 115 Raritan 54 (37.5%) 82 (56.9%) 8 (5.6%) 144 Atlantic 76 (38.6%) 100 (50.8%) 21 (10.6%) 197 Lower Delaware 17 (14.7%) 83 (71.5%) 16 (13.8%) 116

Page 13 of 23 Results and Trends Overall, the bioassessment ratings for each of the monitoring stations are best estimates of the in-stream biological impairment based upon the data obtained in the current AMNET survey. Detailed taxonomic and statistical data, bioassessment ratings, habitat assessment scores and observations for each AMNET site are given in Table 2 and Appendix D, Volume 2.

2004 Raritan Region Bioassessment Results

Non-impaired (160 total sites) 33.8%

Moderately

impaired

63.7%

Severely impaired

2.5%

Figure 2

Figure 3. Map of the Raritan Water Region showing relative stream impairment levels in each Watershed Management Area.

Figure 2 depicts the overall results for the current study in the Raritan Water Region. Of the 160 monitoring stations sampled during this study period, 54 or 33.8% were rated as "non-impaired", 102 or 63.7% were rated as "moderately impaired", and 4 or 2.5% were rated as "severely impaired" (see Table 2, Volume 2). Figure 3 illustrates the proportions of non-impaired, moderately and severely impaired sites in each WMA of the Raritan Water Region for the current AMNET round.

1999 Raritan Region Figure 4 shows the results obtained from 162 Bioassessment Results AMNET sites within the Raritan Water Region (162 total sites) that were sampled during the previous Raritan

Non-impaired study (see “Site Selection” p.6 & Table 2,

35.2% Moderately Volume 2). While the results for 2004 were impaired similar to those for 1999, for the current 55.6% sampling period the numbers of non-impaired Severely impaired and severely impaired sites were slightly lower, 9.2% but the number of moderately impaired sites were slightly higher. The first sampling (1994) of 144 sites yielded more severely impaired sites (5.6%) than in the most recent sampling [4]. Figure 4

Page 14 of 23 Figure 5 displays the percentage change in rating Percent Change in Rating Between the among the same 160 AMNET sites in the Raritan 1999 and the 2004 Monitoring Rounds Water Region that were sampled during the second (160 sites total) (1999) study period [4], and again during the current (2004) study period (see “Site Selection” p.5 Positive Change & Table 2, Volume 2). The green indicates sites that 12.5% have undergone a positive change, yellow indicates No Change no change, and red indicates a negative change. 71.9% Negative Change

Positive change includes both severe to moderate, 15.6% and moderate to non-impairment; negative change includes both non-impairment to moderate and moderate to severe impairment. (see Table 2, Figure 5 Volume 2). As the figure shows, the majority of the 160 sites have exhibited no change between the 2nd and 3rd rounds of sampling.

Regional Results A USGS study, using data generated from NJDEP's AMNET program [15], statistically related levels of impairment to physiographic land types, corresponding land uses, and other anthropogenic factors on a statewide scale. A non-impaired community was most positively related to the area of forested and undeveloped land in its watershed upstream, and to the total underlying terrain in the steeper gradient ecoregions of northwestern New Jersey (i.e., Reading Prong/Highlands). Conversely, a severely impaired community was most positively related to the area of urban land, and to the total volume of wastewater (point source) discharge [15]. The table below presents the proportion of non-impaired, moderately impaired and severely impaired AMNET sites, based on the current data, in each of the Raritan Watershed Management Areas.

WMA’s Sub-basins Non-impaired Moderately Severely Total impaired impaired sites 7 Elizabeth / Rahway / --- 11 (100.0%) --- 11 Woodbridge Rivers system 8 North and South Branch 45 (66.2%) 23 (33.8%) --- 68 Raritan Rivers system 9 Lower Raritan / South 3 (6.8%) 39 (88.6%) 2 (4.6%) 44 Rivers system 10 Millstone River system 6 (16.2%) 29 (78.4%) 2 (5.4%) 37 Totals: 54 (33.8%) 102 (63.7%) 4 (2.5%) 160

In the Raritan Water Region the majority of NJIS scores (63.7%) fall within the "moderately impaired" range; about a third are "non-impaired" and only four sites are rated as "severely impaired" (see Table 2, Volume 2).

Page 15 of 23 Watershed Management Area 7 2004 Bioassessment Results (11 total sites)

Evaluation by WMA Moderately impaired Watershed Management Area #7 includes a total of 12 100% AMNET sites in the Elizabeth and Rahway Rivers and several smaller streams in portions of Essex, Middlesex and Union Counties; these include Robinsons Branch and unnamed tributaries to Robinsons Branch (see Map 2, Volume 2). One site (AN0202) was not sampled due to bridge construction at the site. Figure 6 shows the current site rating summaries for WMA #7 with 100% (11 sites) moderately impaired. Figure 7 depicts the results obtained from 12 sites sampled during the Figure 6 earlier (1999) survey [4]. Comparing the current results to the earlier results, a significant improvement is seen at three sites and a significant decline, at one site (see Table 2, Volume 2). The number of non-impaired and Watershed Management Area 7 severely impaired sites is lower than the earlier data, 1999 Bioassessment Results and the number of moderately impaired sites is slightly (12 total s ites )

Non-impaired increased. 8.3% The majority M oderately impaired (72.7%) of 66.7% habitat scores are in Severely impaired

25.0% the suboptimal range, with 27.3% receiving a Figure 7 marginal score. Abnormalities in chironomid larvae and other invertebrate families were found at four sites (two on on the Rahway River, one on the South Branch Rahway River and one on Robinsons Branch) (see Map 2, Table 3, Volume 2). One of these sites displayed chronic abnormalities (see Table 3, Volume 2). The table below presents a synopsis of AMNET data for WMA #7; AMNET site locations and bioassessment ratings within WMA #7 are shown in Figure 8. Figure 8

WMA # 7 Combined Results Table NJIS Rating 1999 2004 Habitat Assessment 2004 Non-Impaired 1 8.3% ------Optimal ------Moderate 7 66.7% 11100% Suboptimal 8 72.7% Severe 3 25.0% ------Marginal 3 27.3% Poor ------Total sites 12 11 11

Page 16 of 23 Watershed Management Area #8 includes a total of Watershed Management Area 8 68 AMNET sites in the North and South Branch of 2004 Bioassessment Results the Raritan River, and its tributaries, in Hunterdon, (68 total sites) Morris, and Somerset Counties (see Maps 3 & 4, Volume 2). Figure 9 shows the current site rating Non-impaired

summaries for WMA # 8: 66.2% (45 sites) non- 66.2% impaired and 33.8% (23 sites) moderately impaired. Moderately There was one new site (AN0324A) this round. impaired 33.8% Figure 10 depicts the results obtained from 68 sites sampled during the earlier (1999) survey [4]. Comparing the 2004 results to the earlier (1999) Figure 9 results, a significant improvement is apparent at one site while five sites exhibited a decline in impairment rating (see Table 2, Volume 2). The majority (58.8%) of habitat scores are in the suboptimal range with 33.8% Watershed Management Area 8 receiving an 1999 Bioassessment Results (67 total sites) optimal and 7.4% receiving a marginal score. Severely impaired Non-impaired Abnormalities in 1.5% 73.1% chironomid larvae and other invertebrate families were Moderately impaired found at nine sites 25.4% (three on the South Branch Raritan River, two Figure 10 on the Lamington River, and one each on North Branch Raritan River, North Branch Rockaway Creek, Middle Brook, and Peapack Brook) (see Maps 3 and 4, Table 3, Volume 2). Two of these sites displayed chronic abnormalities (see Table 3, Volume 2). The table Figure 11 below presents a synopsis of AMNET data for WMA #8; AMNET site locations and bioassessment ratings within WMA #8 are shown in Figure 11.

WMA # 8 Combined Results Table NJIS Rating 1999 2004 Habitat Assessment 2004 Non-Impaired 49 73.1% 45 66.2% Optimal 23 33.8% Moderate 17 25.4% 2333.8% Suboptimal 40 58.8% Severe 1 1.5% ------Marginal 5 7.4% Poor ------Total sites 67 68 68

Page 17 of 23 Watershed Management Area 9 Watershed Management Area #9 includes a total of 2004 Bioassessment Results (44 total sites) Moderately 46 AMNET sites in the Lower Raritan River, South impaired River and Lawrence Brook and its tributaries in 88.6% Middlessex, Monmouth, Somerset and Union Non-impaired Counties (see Maps 5 and 6, Volume 2). Figure 12 6.8% shows the current site rating summaries for WMA # 9: 6.8% (3 sites) non-impaired, 88.6% (39 sites) Severely impaired 4.6% moderately impaired and 4.6% (2 sites) severely impaired. Two sites (AN0454, AN0455) were not sampled this round. Site AN0454 had bridge Figure 12 construction and it was determined that site AN0455 is a tidal site and therefore would violate the BFBM sampling protocol, so this site will be dropped from the program. Figure 13 depicts the results obtained from 46 sites sampled during the earlier (1999) survey [4]. Comparing the current to the earlier results, a significant improvement is seen at three sites, and a significant decline at two sites (see Table 2, Volume 2). The number of

Watershed Management Area 9 moderately 1999 Bioassessment Results impaired sites (46 total sites) increased slightly

Moderately from that of the Non-impaired impaired earlier sampling, 10.9% 76.1% while the number of non-impaired and severely Severely impaired

13.0% impaired sites is slightly reduced Figure 13 (see Table 2, Volume 2). The majority of sites (77.2%) received a sub-optimal habitat score, with 11.4% receiving an optimal and a marginal score. Abnormalities in chironomid larvae and other invertebrate families were found at 18 sites: one each on Ambrose Brook, Dukes Brook, E Br Middle Brook, Middle Brook, Mill Brook, McGellairds Brook, Peters Brook, Pine Brook, Sawmill Brook, Stony Brook, unnamed tribituary to Raritan River; two each on Figure 14 Manalapan Brook and Matchaponix Brook; and three on Green Brook (see Maps 5 and 6, Table 3, Volume 2). Eight of these sites displayed chronic abnormalities (see Table 3, Volume 2). The table below presents a synopsis of AMNET data for WMA #9; AMNET site locations and bioassessment ratings within WMA # 9 are shown in Figure 14.

WMA # 9 Combined Results Table NJIS Rating 1999 2004 Habitat Assessment 2004 Non-Impaired 5 10.9% 3 6.8% Optimal 5 11.4% Moderate 35 76.1% 3988.6% Suboptimal 34 77.2% Severe 6 13.0% 24.6% Marginal 5 11.4% 46 44 Poor ------Total sites 46 44 44

Page 18 of 23 Watershed Management Area 10 2004 Bioassessment Results Watershed Management Area #10 includes a total of 39 (37 total sites)

AMNET sites in the Millstone River and its tributaries in Non-impaired Moderatel y

Mercer, Middlesex, Monmouth and Somerset Counties 16.2% impaired (see Map 7 , Volume 2). Figure 15 shows the current site 78.4% rating summaries for WMA # 10: 16.2% (6 sites) non- impaired, 78.4% (29 sites) moderately impaired and 5.4% Severely impaired (two sites) severely impaired. Two sites (AN0382D, 5.4% AN0389) were not sampled due to bridge construction. Figure 16 depicts the results obtained from 37 sites Figure 15 sampled during the earlier (1999) survey [4]. Comparing the current to the earlier results, a significant improvement is seen at seven sites (see Table 2, Volume 2). The number of non-impaired sites increased slightly from that of the earlier sampling, and the number of moderately impaired and severely impaired sites is slightly reduced (see Table 2, Volume 2). The majority of sites (62.2%) received a sub-optimal habitat score, with 35.1% receiving an optimal score and 2.7% receiving a marginal score. Abnormalities in chironomid larvae and other invertebrate families were

Watershed Management Area 10 found at seven sites in Mercer, Middlesex and and 1999 Bioassessment Results Somerset Counties: one each on Bear Brook, Cranbury (37 total sites)

Non-impaired

5.4% Moderatel y

impaired

81.1% Severely impaired

13.5%

Figure 16

Brook, Cruser Brook, Heathcote Brook, Millstone River, Six Mile Run and Ten Mile Run (see Map 7, Table 3, Volume 2). Three of these sites displayed chronic abnormalities (see Table 3, Volume 2). The table below presents a synopsis of AMNET data for WMA #10; AMNET site locations and bioassessment ratings within WMA # 10 are shown in Figure 17.

Figure 17 WMA # 10 Combined Results Table NJIS Rating 1999 2004 Habitat Assessment 2004 Non-Impaired 2 5.4% 6 16.2% Optimal 13 35.1% Moderate 30 81.1% 2978.4% Suboptimal 23 62.2% Severe 5 13.5% 25.4% Marginal 1 2.7% Poor ------Total sites 37 37 37

Page 19 of 23 Macroinvertebrate Abnormalities Occasionally, morphological abnormalities have been found in individual macroinvertebrates recovered in the BFBM AMNET collections. These deformities have been most often detected in larval organisms belonging to the insect family Chironomidae (midges), where they occur primarily in the head appendages (antennae) and mouthparts (mentum and mandibles). Abnormalities have also been observed in individuals of other taxonomic groups (such as Amphipoda), but they are most often noted in the mouthparts and antennae of Chironomidae because these features are key characteristics used in identification. Chironomidae larvae often comprise a large component of the benthic community of a stream or river, particularly in those affected by human disturbances, and they are part of the diet of predatory invertebrates and fish. As a result, chironomids are an important transfer vector linking the movement of contaminants from sediments to higher trophic levels [16].

Hamilton and Saether [17] noted deformed specimens (Chironomidae) occurred in areas of industrial or agricultural chemical input, but not in areas receiving only domestic effluents. Subsequent studies have supported this finding. But the presence of deformed organisms in a sample is difficult to interpret. Not all genera appear to react to the presence of contaminants in the same manner [18]. Most of the research has been focused on a few genera. The North Carolina Division of Environmental Management [19] has developed an index to evaluate deformities, using the frequency and severity of deformities observed in Chironomidae larvae of just the genus Chironomus. Morphological deformities undoubtedly occur in Chironomidae larvae living in uncontaminated environments. Even robust, healthy populations of any fauna are likely to include a certain proportion of physiologically weaker individuals which, for various reasons, may be more prone or genetically predisposed to malformation [18]. With a lack of baseline data of deformities in more pristine environments, the level at which these deformities becomes significant is somewhat uncertain. Currently, although not an indicator of specific contaminants, the occurrence of abnormal chironomid larvae can serve as an economical and long-term monitor of the benthic environment, and can suggest where more intensive bioassays and chemical testing would be most effectively employed [20].

Bearing in mind that the primary focus of the AMNET sampling is not to find morphological abnormalities, a listing of all AMNET sites in the Raritan Water Region exhibiting these deformities is presented in Table 3 (Volume 2). The data are displayed as # of chironomids with abnormalities / # of chironomids examined. For all other taxa, just the number of individuals with abnormalities is presented. The significance of these abnormalities has not been statistically evaluated. Deformities are called “chronic” if they were observed in more than one round of sampling at a given site. Also, the presence of abnormalities is not factored into the NJIS but, rather, used to identify sites where additional investigations are needed.

Fewer abnormalities are seen in the current sampling than in the previous (1999) sampling [4]. From the current sampling of 160 sites, 34 (21.3%) contained organisms with abnormalities (Maps 2 - 7 , Volume 2). 14 of the sites exhibited a "chronic" presence of abnormalities (Table 3, Volume 2). Notably, the majority of these "chronic" sites are situated in areas where a higher percentage of urban land use occurs (Maps 2 - 7, Volume 2). Further study is needed to establish the significance of the presence of abnormalities.

Causes and Conditions of Impairment Biological impairment, as determined through RBP analysis, is manifested by alterations or differences in

Page 20 of 23 macroinvertebrate community structure, compared to a reference or "ideal" condition. In an impaired situation, taxa of pollution-tolerant groups (such as worms and midges) tend to dominate over pollution- intolerant forms (e.g., mayflies, stoneflies, etc.), with an overall depression in species diversity. Such discrepancies are typically due to degraded instream environmental conditions, which may be caused by various human activities or land uses and, in some cases, by natural features or events. Environmental factors that may adversely affect stream biology, including both chemical and physical parameters, are listed below:

1. Degraded habitat (see Table 4, Volume 2) a. lack of stable and varied substrate b. lack of bank vegetation/canopy (= poor bank stability, lack of shade) c. excessive sedimentation (= poor substrate and water clarity) d. lack of streamflow (= low water level, low dissolved oxygen, possible sedimentation, undesirable vegetation) 2. Eutrophication (= excessive nutrients promoting undesirable vegetation or algal blooms, and increased turbidity) 3. Domestic (organic) waste (promotes hypoxia, turbidity, eutrophication) 4. Physiochemical water quality factors which, alone or in combination, can have adverse effects a. higher than normal temperature b. excessive turbidity c. lack of dissolved oxygen d. presence of toxicants (in various chemical forms)

Inter-related human activities or practices, land uses, and natural features or events that contribute to degraded stream quality include:

1. Deforestation/development/construction (largely via runoff from non-point sources) 2. Urbanization/industrialization (largely via runoff from non-point sources) 3. Agricultural operations (largely via runoff from non-point sources) 4. Municipal or industrial wastewater discharge (from point sources) 5. Artificial channelization or habitat alteration 6. Upstream impoundment, lake or pond 7. Drought conditions

Habitat Assessment vs. Biological Impairment The relationship between habitat assessment scores and corresponding NJIS scores were plotted, and a coefficient of determination (R2) value calculated for each WMA (Appendix C, Volume 2). The R2 has a value ranging from zero to one, and is a fraction of the variance shared by two variables graphed along an X and Y axis. For example, if R2 = 0.59, then 59% of the variance in X can be explained by the variance in Y, or vice versa. The higher the R2 value, the more likely the variance in one variable can be explained by the variance of another. In this case the variables are habitat assessments vs. biological impairment (NJIS). The R2 values were calculated to determine if general trends in habitat degradation could explain general trends in biological impairment. For all sites in the Raritan Water Region, an overall R2 value of 0.14 was calculated when comparing habitat assessments to NJIS. This can be interpreted that for all sites in this region, a strong direct correlation between habitat and biological impairment existed 14% of the time. An R2 value was also calculated, individually, for the three WMA's in this Water Region. The R2

Page 21 of 23 values for WMA 7, 8, 9, and 10, were 0.26, 0.13, 0.13, and 0.02 respectively. Again, this indicates that a strong direct correlation between habitat and biological impairment existed 2% - 26% of the time.

The R2 values suggest that other factors, which may include land use and/or water quality, are likely contributing to the observed biological impairments. Sites with an impaired biological assessment, but with a relatively high habitat assessment score, could be impacted by point and/or nonpoint sources outside the range of the visual based habitat assessment. Also, an intermittent or short term impact may have occurred which left no obvious visual evidence at the site. In these cases, further investigation is needed to determine the source of impairment that is affecting the biota. Some sites assessed with a non-impaired biological assessment may have a relatively degraded habitat assessment. This could be due to a temporary degradation, such as drought or flooding (near to the time of the assessment), which was not severe enough to effect the biota. It is also possible that a temporary or recent degradation may not have immediate observable effects on the biota. In either case, these sites should be studied further to avoid future impairment to the biota. Due to the prevalence of multiple stressors throughout the State, it is further suggested that the relationship between habitat assessments and biological assessments be studied on a site-by-site basis.

As reflected in the present study results, human land uses and practices, superimposed on the undisturbed physical terrain, play a major role in controlling the degree of pollution or degradation in a stream system [15]. The relationship between benthic macroinvertebrate community impairment has been statistically related to different physiographic land types, land uses and other anthropogenic factors, on a statewide basis [15]. These findings strongly indicate that human land uses and practices play a major role in the degree of pollution or degradation in a stream system. Data analysis from Ayers et al., 2000 [21] for instance, concludes the following: 1) Fish and invertebrate communities are commonly impaired in urban streams; 2) Invertebrate community impairment was related to total urban land and total wastewater flow upstream of a site; 3) Changes in aquatic community structure were statistically related to environmental variables along the urban gradient – that is to say that such things as impervious surfaces were related to a negative response in the aquatic invertebrate community.

Conversely, the same Ayers data analysis also demonstrated that the area of forest and wetland in a stream’s drainage basin was a strong mitigating factor in protecting invertebrate community health.

Additional Information

For more information, please contact:

Department of Environmental Protection Alfred Korndoerfer, Jr., Chief Water Monitoring & Standards Bureau of Freshwater and Biological Monitoring P. O. Box 427 Trenton, NJ 08625-0427 (609) 292-0427 http://www.state.nj.us/dep/wms/bfbm

Page 22 of 23

REFERENCES

1. Plafkin, J.L., M.T. Barbour, K.D. Porter, S.K. Gross and R.M. Hughes, 1989. Rapid bioassessment protocols for use in streams and rivers—benthic macroinvertebrates and fish. EPA/44/4-89-002. US Environmental Protection Agency. Washington, D.C. 143pp. and appendices. 2. New Jersey Department of Environmental Protection. 2006. New Jersey 2006 Integrated Water Quality Monitoring and Assessment Report, 305 (b) and 303 (d). Water Monitoring and Standards. Trenton, NJ. 3. New Jersey Department of Environmental Protection. Data report, 1998. New Jersey’s modernized ambient chemical monitoring network. Division of Watershed Management. Trenton, NJ. 12pp. 4. New Jersey Department of Environmental Protection. Data report, 1995. Ambient biomonitoring network, Raritan River drainage basins. Bureau of Water Monitoring. Trenton, NJ. 8pp. and maps and appendices. 5. New Jersey Department of Environmental Protection. Data report, 2000. Ambient biomonitoring network Raritan Region. Bureau of Water Monitoring. Trenton, NJ. 16pp. and maps and appendices. 6. New Jersey Department of Environmental Protection. Data report, 1994. Ambient biomonitoring network, Arthur Kill, Passaic, Hackensack, and Wallkill River drainage basins. Bureau of Water Monitoring. Trenton, NJ. 10pp. and maps and appendices. 7. New Jersey Department of Environmental Protection. 1992. Field sampling procedures manual. NJDEP. Trenton, NJ. 360pp. 8. Barbour, M.T., J. Gerritson, B.D. Snyder and J.B. Stribling. 1999. Rapid bioassessment protocols for use in wadable streams and rivers: Periphyton, Benthic Macroinverbrates, and Fish, 2nd ed. USEPA 841-B-99-002. Chps. 1–11 and appendices. 9. New Jersey Department of Environmental Protection. Laboratory report, 1998. Standard operating procedures for the aquatic biomonitoring laboratory. Bureau of Freshwater & Biological Monitoring. Trenton, NJ. 10. New Jersey Department of Environmental Protection. Report, 2004. Work/quality assurance project plan: Ambient Biomonitoring Network (AMNET), Raritan Region, FY04-05. Bureau of Freshwater and Biological Monitoring. Trenton, NJ. 11. U.S. Environmental Protection Agency. 1997. Field and laboratory methods for macroinvertebrate and habitat assessment of low gradient nontidal streams. Mid-Atlantic Coastal Streams Workgroup, Environmental Services Division, Region 3. Wheeling, WV. 23pp. and appendices. 12. Klemm, D.J., P.A. Lewis, F. Fulk and J.M. Lazorchak. 1990. Macroinvertebrate field and laboratory methods for evaluating the biological integrity of surface waters. EPA/600/4-90/030. U.S. Environmental Protection Agency. Cincinnati, OH. 206pp. and appendices. 13. Kurtenbach, J. 1991. A method for rapid bioassessment of streams in New Jersey using benthic macroinvertebrates. Bull. N. Am. Benth. Soc. 8(1):129. 14. New Jersey Department of Environmental Protection. 2006. Surface and Ground Water Quality Standards. Water Monitoring and Standards. Trenton, NJ. 15. Kennen, J.G. 1998. Relation of benthic macroinvertebrate community impairment to basin characteristics in New Jersey streams. Fact Sheet FS-057-98. U.S. Geological Survey. West Trenton, NJ. 16. Dickman, Mike, Ian Brindle, and Martin Benson, 1992. Evidence of teratogens in sediments of the Niagara River Watershed as reflected by chironomid (Diptera: Chironomidae) deformities. Journal of Great Lakes Res. 18(3):467- 480. 17. Hamilton, A.L. and O.A. Saether, 1971. The occurrence of characteristic deformities in the chironomid larvae of several Canadian lakes. Canadian Entomologist 103:363-368. 18. Warwick, W.F., 1985. Morphological abnormalities in Chironomidae (Diptera) larvae as measures of toxic stress in freshwater ecosystems: indexing antennal deformities in Chironomus Meigen. Canadian Journal of Fisheries and Aquatic Sciences 42:1881-1914. 19. Lenat, David R., 1993. Using mentum deformities of Chironomus larvae to evaluate the effects of toxicity and organic loading in streams. Journal of N. Am. Benthol. Soc. 12(3):265-269. 20. Diggins, T.P. and K.M. Stewart, 1993. Deformities of aquatic larval midges (Chironomidae: Diptera) in the sediments of the Buffalo River, New York. Journal of Great Lakes Res. 19(4):648-659 21. Ayers, M., Kennon, J., Stackleberg, P., Kauffman, L. 2000. Building a stronger scientific basis for landuse planning and watershed management effects on water quality and aquatic communities in NJ streams. USGS. West Trenton, NJ.

Page 23 of 23 TABLE 1

BIOLOGICAL CRITERIA FOR SCREENING WATER QUALITY IN NEW JERSEY FRESHWATER STREAMS*

Scoring Criteria for Rapid Bioassessments1

Biometrics 6 3 0

Taxa Richness (total Families) >10 10-5 4-0

E+P+T Index2 (EPT) >5 5-3 2-0

Percent Dominance3 (%CDF) <40 40-60 >60

Percent EPT4 (%EPT) >35 35-10 <10

Modified Family Biotic Index5 (FBI) <5 5-7 >7

NOTE: The previous AMNET reports (1994-1996) contained incorrect number ranges for Modified Family Biotic Index. Using the incorrect numbers could lower the biological assessment on 9% of the sites evaluated. The numbers now presented in this table are correct and scores from previous reports were calculated using these ranges. No incorrect biological assessments exist in the previous reports.

Biological Assessment Total Score

Non-impaired 24-30

Moderately Impaired 9-21

Severely Impaired 0-6

Attributes

Non-impaired: benthic community comparable to other undisturbed streams within the region; community characterized by a maximum taxa richness, balanced taxa groups, and good representation of intolerant individuals.

Moderately Impaired: macroinvertebrate richness reduced, in particular EPT taxa; reduced community balance and numbers of intolerant taxa.

Severely Impaired: benthic community dramatically different from those in less impaired situations; macroinvertebrates dominated by a few taxa, but with many individuals; only tolerant individuals present.

* 1From Kurtenbach, 1991, based on RBP II protocols. 2 Follows RBP Protocol II; using 100 organism subsample, family level taxonomy 3Ephemeroptera, Plecoptera, Trichoptera 4% contribution of the dominant family 5 Including the hydropsychid family Also known as the Hilsenhoff Biotic Index

NJ Department of Environmental Protection Water Monitoring and Standards

AMBIENT BIOMONITORING NETWORK

Raritan Water Region

Watershed Management Areas 7, 8, 9, and 10 Round 3 Benthic Macroinvertebrate Data Volume 2 of 2

February 2008

State of New Jersey NJ Department of Environmental Protection Jon S. Corzine, Governor Lisa Jackson, Commissioner

NJ Department of Environmental Protection Land Use Management Mark Mauriello, Assistant Commissioner

Water Monitoring and Standards Leslie McGeorge, Administrator

Bureau of Freshwater & Biological Monitoring Alfred L. Korndoerfer, Jr., Chief

February 2008

AMBIENT BIOMONITORING NETWORK

Raritan Water Region Watershed Management Areas 7, 8, 9, and 10

Round 3 Benthic Macroinvertebrate Data

Volume 2 of 2

Water Monitoring Report Prepared By: Bureau of Freshwater and Biological Monitoring

Sampling and Data Analysis: Victor Poretti, Project Manager-Sampling Coordination Dean Bryson, Project Manager-Laboratory Operations Jessica Messersmith Thomas Miller Anna Signor

Report Preparation: Thomas Miller

Map Preparation: John Sell

Edited By: Alfred Korndoerfer Leslie McGeorge Alena Baldwin-Brown

AMBIENT BIOMONITORING NETWORK Watershed Management Areas 7, 8, 9, and 10

Raritan Water Region

Round 3 Benthic Macroinvertebrate Data

Volume 2 of 2

TABLE OF CONTENTS

page MAPS (AMNET Site Locations) Raritan Water region Map 1 Watershed Management Area # 7 Map 2 Watershed Management Area # 8 Maps 3, 4 Watershed Management Area # 9 Maps 5, 6 Watershed Management Area # 10 Map 7 TABLE 2. Comparative Scores / Ratings TABLE 3. Macroinvertebrates Abnormalities TABLE 4. Habitat Assessment TABLE 5. List of AMNET sites with Parameters that did not attain standards APPENDIX A. Station Numbers and Locations A APPENDIX B. Pictures of Morphological Abnormalities B APPENDIX C. Graphical Comparison of Habitat Assessment and New Jersey C Impairment Scores APPENDIX D. Taxonomic and Statistical Data, NJIS Scores, Habitat D Assessment Scores and Observations

i

MAPS

Round 3 Raritan Region AMNET Study WMA's 7, 8, 9, & 10

AMNET site locations and their respective biological ratings, for each major sub-basin, are shown in maps 1- 7. Also identified are sites that exhibited significant and chronic macroinvertebrate abnormalities.

Table 2

Comparative Scores / Ratings (see notes)

Watershed Management Areas 7, 8, 9 and 10

Station NJ Impairment Change Habitat WMA Station NJ Impairment Change Habitat WMA Station NJ Impairment Change Habitat WMA Score in Score Score in Score Score in Score 1999 2004 Rating 1999 2004 Rating 1999 2004 Rating

192 15 12 /- 58 7 338 30 27 /- 137 8 379 18 6 — 150 10 193 9 18 /+ 152 7 339 30 30 / 156 8 380 18 21 /+ 148 10 194 3 12 + 107 7 340 21 24 + 110 8 381 6 15 + 108 10 195 6 18 + 134 7 341 27 21 — 135 8 382 21 15 /- 158 10 196 12 9 /- 101 7 342 30 30 / 152 8 382B 15 15 / 133 10 197 15 18 /+ 125 7 343 21 15 /- 111 8 382D 12 nd nd nd 10 198 18 12 /- 111 7 344 30 21 — 160 8 383 12 12 / 160 10 199 15 15 / 116 7 344A 27 30 /+ 115 8 384 24 12 — 170 10 200 6 18 + 113 7 345 30 30 / 189 8 385 6 3 /- 146 10 201 12 18 /+ 131 7 346 30 24 /- 162 8 386 nd 9 nd 158 10 202 12 nd nd nd 7 347 30 30 / 145 8 387 6 15 + 166 10 204 24 21 — 102 7 348 30 30 / 163 8 388 15 12 /- 146 10 310 15 15 / 178 8 349 30 30 / 167 8 389 21 nd nd nd 10 311 21 21 / 124 8 350 30 30 / 142 8 390 15 21 /+ 162 10 312 30 21 — 153 8 351 30 27 /- 147 8 391 12 27 + 167 10 313 30 30 / 164 8 352 6 18 + 147 8 392 21 24 + 154 10 314 30 12 — 101 8 353 21 24 + 115 8 393 12 21 /+ 173 10 315 24 24 / 147 8 354 30 9 — 136 8 394 18 9 /- 141 10 316 27 24 /- 178 8 355 24 27 /+ 130 8 395 15 12 /- 126 10 317 30 30 / 183 8 356 9 9 / 119 8 396 24 18 — 168 10 318 30 30 / 178 8 357 27 24 /- 106 8 397 9 18 /+ 170 10 319 24 21 — 162 8 358 27 21 — 191 8 398 21 15 /- 165 10 320 27 30 /+ 174 8 359 30 30 / 166 8 399 18 27 + 171 10 321 24 21 — 177 8 360 30 30 / 157 8 400 nd 24 nd 128 10 322 27 27 / 111 8 361 30 30 / 161 8 401 15 27 + 143 10 323 30 27 /- 184 8 362 30 30 / 137 8 402 18 24 + 161 10 324 9 12 /+ 103 8 363 30 30 / 156 8 403 21 21 / 153 10 324A nd 30 nd 159 8 364 30 27 /- 154 8 404 21 21 / 121 10 325 30 27 /- 157 8 365 30 30 / 144 8 405 3 15 + 150 10 325B 30 30 / 150 8 366 30 30 / 180 8 406 15 12 /- 112 10 326 18 30 + 143 8 367 30 21 — 165 8 407 21 18 /- 155 10 327 30 30 / 167 8 368 21 15 /- 171 8 408 15 9 /- 149 10 328 30 30 / 178 8 369 27 15 — 104 8 409 18 15 /- 140 10 329 27 24 /- 145 8 370 30 27 /- 137 8 410 15 15 / 157 10 330 12 12 / 142 8 371 18 15 /- 128 8 411 21 18 /- 160 10 331 21 27 + 137 8 372 24 27 /+ 139 8 412 12 12 / 162 10 332 21 30 + 129 8 373 30 18 — 163 8 413 6 9 + 131 10 333 12 21 /+ 130 8 374 30 30 / 156 8 414 15 21 /+ 123 10 334 18 21 /+ 112 8 375 21 12 /- 130 9 415 15 12 /- 134 9 335 12 27 + 108 8 376 9 15 /+ 113 9 416 18 9 /- 158 9 336 27 30 /+ 154 8 377 21 12 /- 118 9 417 12 18 /+ 152 9 337 18 21 /+ 146 8 378 9 18 /+ 112 10 418 18 15 /- 157 9

NOTES:

Comparison of NJ impairment score results between earliest and latest sampling dates:

nd no data + indicates positive change in rating — indicates negative change in rating / indicates no change in rating /+ or /- indicates change in score, but not in rating (see Table 1)

NJ Impairment Score Value Habitat Score Value Non-Impaired 24 - 30 Optimal 160 - 200 Moderately Impaired 9 - 21 Sub-optimal 110 - 159 Severely Impaired 0 - 6 Marginal 60 - 109 Poor <60

Table 2

Comparative Scores / Ratings (see notes)

Watershed Management Areas 7, 8, 9 and 10

Station NJ Impairment Change Habitat WMA Station NJ Impairment Change Habitat WMA Station NJ Impairment Change Habitat WMA Score in Score Score in Score Score in Score 1999 2004 Rating Rating Rating

419 27 15 — 157 9 420 30 21 — 169 9 421 15 15 / 118 9 422 15 15 / 158 9 423 9 18 /+ 122 9 424 12 15 /+ 134 9 424B 6 12 + 82 9 425 15 12 /- 168 9 425A 18 15 /- 100 9 426 15 3 — 105 9 427 18 12 /- 134 9 428 24 18 — 135 9 429 6 12 + 126 9 430 6 9 + 156 9 431 12 15 /- 115 9 432 15 18 /+ 167 9 433 21 15 /- 190 9 434 15 15 / 149 9 435 12 9 /- 76 9 436 15 24 + 155 9 437 27 24 /- 158 9 438 27 12 — 143 9 439 18 24 + 110 9 440 21 15 /- 138 9 441 9 12 /+ 134 9 442 21 12 /- 148 9 443 15 15 / 141 9 444 15 21 /+ 134 9 445 12 12 / 122 9 446 15 15 / 127 9 447 6 9 + 127 9 448 15 15 / 133 9 449 6 6 / 115 9 450 21 12 /- 146 9 451 12 9 /- 113 9 452 15 9 /- 78 9 453 15 9 /- 122 9 454 6 nd nd nd 9 455 15 nd nd nd 9

NOTES:

Comparison of NJ impairment score results between earliest and latest sampling dates:

nd no data + indicates positive change in rating — indicates negative change in rating / indicates no change in rating /+ or /- indicates change in score, but not in rating (see Table 1)

NJ Impairment Score Value Habitat Score Value Non-Impaired 24 - 30 Optimal 160 - 200 Moderately Impaired 9 - 21 Sub-optimal 110 - 159 Severely Impaired 0 - 6 Marginal 60 - 109 Poor <60

Table 3

Macroinvertebrate Abnormalities (see notes)

Watershed Management Areas 7, 8, 9, and 10

Station 1999 2004 WMA Station 1999 2004 WMA

193 1/15+1* 7 393 1/44 10 194 +2 +1 7 395 4/16* 10 195 +3 7 396 1/36 10 197 1/3* 7 397 1/58 10 199 +1 7 401 2/52 10 200 1/8 7 403 2/44 2/18 10 202 1/26 7 405 3/31* 10 204 3/26* 7 409 1/2* +1 10 311 +1 8 411 1/1* 10 315 +1 8 417 2/29* 9 316 1/48 8 418 1/14* 9 322 2/44 8 419 1/19 1/43 9 325B 1/35 8 420 3/15* 2/41 9 330 5/82* 8 421 +1, 3/19 9 332 +1 8 422 1/23 9 334 +3 8 423 1/43 9 337 +1 8 424 2/3* 9 343 1/33 8 424B +1 9 348 1/10* 8 425A +1 9 349 1/9 8 426 +1 3/70 9 350 1/26 8 427 1/19 9 353 1/38 8 428 1/21 9 355 2/37 8 431 1/24 9 356 +1 +2 8 435 1/14 9 358 +1 1/38 8 436 1/26 9 366 1/15 8 438 4/12* 9 372 2/29* 8 439 1/22 9 374 +1 8 440 1/59 9 375 1/22 +1 9 445 3/25* 9 376 3/15* +1 9 447 +1 9 379 1/28 10 448 3/23*+1 +2 9 382 +2 10 449 3/48* 1/60 9 382B +1 10 450 2/25* 9 384 2/21* 1/53 10 451 +1 1/70 9 385 3/114 10 452 1/17* 9 392 1/8* 10

NOTES: # chironomids with deformities / # chironomids examined

+ — indicates the number of non-chironomids having abnormalities

abnormalities are considered chronic if they appear in both the 1999 and the 2004 columns

Table 4 — HABITAT ASSESSMENT FOR HIGH GRADIENT STREAMS

Habitat Condition Category Parameter Optimal Suboptimal Marginal Poor

Greater than 70% of substrate 40-70% mix of stable habitat; 20-40% mix of stable habitat; Less than 20% stable habitat; lack 1. Epifaunal favorable for epifaunal well suited for full colonization habitat availability less than of habitat is obvious; substrate Substrate/Available colonization and fish cover; mix potential; adequate habitat for desirable; substrate frequently unstable or lacking. Cover of snags, submerged logs, maintenance of populations; disturbed or removed. undercut banks, cobble or other presence of additional substrate in stable habitat and at stage to the form of newfall, but not yet allow full colonization potential prepared for colonization (may (i.e., logs/snags that are not new rate at high end of scale). fall and not transient). SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Gravel, cobble, and boulder Gravel, cobble, and boulder Gravel, cobble, and boulder Gravel, cobble, and boulder 2. Embeddedness particles are 0-25% surrounded by particles are 25-50% surrounded particles are 50-75% surrounded particles are more than 75% fine sediment. by fine sediment. by fine sediment. surrounded by fine sediment. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

All 4 velocity/depth regimes Only 3 of the 4 regimes present Only 2 of the 4 habitat regimes Dominated by 1 velocity / depth 3. Velocity/Depth present (slow-deep, slow-shallow, (if fast-shallow is missing, score present (if fast-shallow or slow- regime (usually slow-deep). Regimes fast-deep, fast-shallow). lower than if missing other shallow are missing, score low). (slow is <0.3 m/s, deep is regimes). >0.5 m) SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Little or no enlargement of Some new increase in bar Moderate deposition of new Heavy deposits of fine material, 4. Sediment Deposition islands or point bars and less than formation, mostly from gravel, gravel, sand or fine sediment on increased bar development; more 5% (<20% for low-gradient sand or fine sediment; 5-30% (20- old and new bars; 30-50% (50- than 50% (80% for low-gradient) streams) of the bottom affected by 50% for low-gradient) of the 80% for low-gradient) of the of the bottom changing sediment deposition. bottom affected; slight deposition bottom affected; sediment frequently; pools almost absent in pools. deposits at obstructions, due to substantial sediment constrictions, and bends; deposition. moderate deposition of pools prevalent. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Water reaches base of both lower Water fills >75% of the available Water fills 25-75% of the Very little water in channel and 5. Channel Flow Status banks, and minimal amount of channel; or <25% of channel available channel, and/or riffle mostly present as standing pools. channel substrate is exposed. substrate is exposed. substrates are mostly exposed. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Channelization or dredging absent Some channelization present, Channelization may be extensive; Banks shored with gabion or 6. Channel Alteration or minimal; stream with normal usually in areas of bridge embankments or shoring cement; over 80% of the stream pattern. abutments; evidence of past structures present on both banks; reach channelized and disrupted. channelization, i.e., dredging, and 40 to 80% of stream reach In stream habitat greatly altered or (greater than past 20 yrs.) may be channelized and disrupted. removed entirely. present, but recent channelization is not present. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Occurrence of riffles relatively Occurrence of riffles infrequent; Occasional riffle or bend; bottom Generally all flat water or shallow 7. Frequency of Riffles (or frequent; ratio of distance distance between riffles divided contours provide some habitat; riffles; poor habitat; distance bends) between riffles divided by width by the width of the stream is distance between riffles divided between riffles divided by the of the stream <7:1 (generally 5 to between 7 to 15. by the width of the stream is width of the stream is a ratio of 7); variety of habitat is key. In between 15 to 25. >25. streams where riffles are continuous, placement of boulders or other large, natural obstruction is important. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Banks stable; evidence of erosion Moderately stable; infrequent, Moderately unstable; 30-60% of Unstable; many eroded areas; 8. Bank Stability (score or bank failure absent or minimal; small areas of erosion mostly bank in reach has areas of "raw" areas frequent along each bank) little potential for future healed over. 5-30% of bank in erosion; high erosion potential straight sections and bends; Note: determine left problems. <5% of bank affected. reach has areas of erosion. during floods. obvious bank sloughing; 60-100% or right side by facing of bank has erosional scars. downstream. SCORE ___ (LB) Left Bank 10 9 8 7 6 5 4 3 2 1 0 SCORE ___ (RB) Right Bank 10 9 8 7 6 5 4 3 2 1 0

More than 90% of the streambank 70-90% of the streambank 50-70% of the streambank Less than 50% of the streambank 9. Bank Vegetative surfaces and immediate riparian surfaces covered by native surfaces covered by vegetation; surfaces covered by vegetation; Protection (score each zone covered by native vegetation, but one class of plants disruption obvious; patches of disruption of streambank bank) vegetation, including trees, under is not well-represented; disruption bare soil or closely cropped vegetation is very high; story shrubs, or nonwoody evident but not affecting full plant vegetation common; less than vegetation has been removed to 5 macrophytes; vegetative growth potential to any great one-half of the potential plant centimeters or less in average disruption through grazing or extent; more than one-half of the stubble height remaining. stubble height. mowing minimal or not evident; potential plant stubble height almost all plants allowed to grow remaining. naturally. SCORE ___ (LB) Left Bank 10 9 8 7 6 5 4 3 2 1 0 SCORE ___ (RB) Right Bank 10 9 8 7 6 5 4 3 2 1 0

Width of riparian zone >18 Width of riparian zone 12-18 Width of riparian zone 6-12 Width of riparian zone <6 meters: 10. Riparian Vegetative meters; human activities (i.e., meters; human activities have meters; human activities have little or no riparian vegetation due Zone Width (score each parking lots, roadbeds, clear-cuts, impacted zone only minimally. impacted zone a great deal. to human activities. bank riparian zone) lawns, or crops) have not impacted zone. SCORE ___ (LB) Left Bank 10 9 8 7 6 5 4 3 2 1 0 SCORE ___ (RB) Right Bank 10 9 8 7 6 5 4 3 2 1 0

HABITAT SCORES VALUE OPTIMAL 160 Χ 200 SUB-OPTIMAL 110 Χ 159 MARGINAL 60 Χ 109 POOR < 60

Table 4 (cont.) — HABITAT ASSESSMENT FOR LOW GRADIENT STREAMS

Habitat Condition Category Parameter Optimal Suboptimal Marginal Poor

Greater than 50% of substrate 30-50% mix of stable habitat; 10-30% mix of stable habitat; Less than 10% stable habitat; lack 1. Epifaunal favorable for epifaunal well suited for full colonization habitat availability less than of habitat is obvious; substrate Substrate/Available colonization and fish cover; mix potential; adequate habitat for desirable; substrate frequently unstable or lacking. Cover of snags, submerged logs, maintenance of populations; disturbed or removed. undercut banks, cobble or other presence of additional substrate in stable habitat and at stage to the form of newfall, but not yet allow full colonization potential prepared for colonization (may (i.e., logs/snags that are not new rate at high end of scale). fall and not transient). SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Mixture of substrate materials, Mixture of soft sand, mud, or All mud or clay or sand bottom; Hard-pan clay or bedrock; no root 2. Pool Substrate with gravel and firm sand clay; mud may be dominant; little or no root mat; no mat or vegetation. Characterization prevalent; root mats and some root mats and submerged submerged vegetation. submerged vegetation common. vegetation present. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Even mix of large-shallow, large- Majority of pools large-deep; very Shallow pools much more Majority of pools small-shallow 3. Pool Variability deep, small-shallow, small-deep few shallow. prevalent than deep pools. or pools absent. pools present. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Little or no enlargement of Some new increase in bar Moderate deposition of new Heavy deposits of fine material, 4. Sediment Deposition islands or point bars and less than formation, mostly from gravel, gravel, sand or fine sediment on increased bar development; more 5% <20% for low-gradient sand or fine sediment; 5-30% (20- old and new bars; 30-50% (50- than 50% (80% for low-gradient) streams) of the bottom affected by 50% for low-gradient) of the 80% for low-gradient) of the of the bottom changing sediment deposition. bottom affected; slight deposition bottom affected; sediment frequently; pools almost absent in pools. deposits at obstructions, due to substantial sediment constrictions, and bends; deposition. moderate deposition of pools prevalent. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Water reaches base of both lower Water fills >75% of the available Water fills 25-75% of the Very little water in channel and 5. Channel Flow Status banks, and minimal amount of channel; or <25% of channel available channel, and/or riffle mostly present as standing pools. channel substrate is exposed. substrate is exposed. substrates are mostly exposed. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Channelization or dredging absent Some channelization present, Channelization may be extensive; Banks shored with gabion or 6. Channel Alteration or minimal; stream with normal usually in areas of bridge embankments or shoring cement; over 80% of the stream pattern. abutments; evidence of past structures present on both banks; reach channelized and disrupted. channelization, i.e., dredging, and 40 to 80% of stream reach In stream habitat greatly altered or (greater than past 20 yrs.) may be channelized and disrupted. removed entirely. present, but recent channelization is not present. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

The bends in the stream increase The bends in the stream increase The bends in the stream increase Channel straight; waterway has 7. Channel Sinuosity the stream length 3 to 4 times the stream length 2 to 3 times the stream length 2 to 1 times been channelized for a long longer than if it was in a straight longer than if it was in a straight longer than if it was in a straight distance. line. (Note - channel braiding is line. line. considered normal in coastal plains and other low-lying areas. This parameter is not easily rated in these areas. SCORE 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

Banks stable; evidence of erosion Moderately stable; infrequent, Moderately unstable; 30-60% of Unstable; many eroded areas; 8. Bank Stability (score or bank failure absent or minimal; small areas of erosion mostly bank in reach has areas of "raw" areas frequent along each bank) little potential for future healed over. 5-30% of bank in erosion; high erosion potential straight sections and bends; problems. <5% of bank affected. reach has areas of erosion. during floods. obvious bank sloughing; 60-100% of bank has erosional scars. SCORE ___ (LB) Left Bank 10 9 8 7 6 5 4 3 2 1 0 SCORE ___ (RB) Right Bank 10 9 8 7 6 5 4 3 2 1 0

More than 90% of the streambank 70-90% of the streambank 50-70% of the streambank Less than 50% of the streambank 9. Bank Vegetative surfaces and immediate riparian surfaces covered by native surfaces covered by vegetation; surfaces covered by vegetation; Protection (score each zone covered by native vegetation, but one class of plants disruption obvious; patches of disruption of streambank bank) vegetation, including trees, under is not well-represented; disruption bare soil or closely cropped vegetation is very high; story shrubs, or nonwoody evident but not affecting full plant vegetation common; less than vegetation has been removed to 5 Note: determine left macrophytes; vegetative growth potential to any great one-half of the potential plant centimeters or less in average or right side by facing disruption through grazing or extent; more than one-half of the stubble height remaining. stubble height. downstream. mowing minimal or not evident; potential plant stubble height almost all plants allowed to grow remaining. naturally. SCORE ___ (LB) Left Bank 10 9 8 7 6 5 4 3 2 1 0 SCORE ___ (RB) Right Bank 10 9 8 7 6 5 4 3 2 1 0

Width of riparian zone >18 Width of riparian zone 12-18 Width of riparian zone 6-12 Width of riparian zone <6 meters: 10. Riparian Vegetative meters; human activities (i.e., meters; human activities have meters; human activities have little or no riparian vegetation due Zone Width (score each parking lots, roadbeds, clear-cuts, impacted zone only minimally. impacted zone a great deal. to human activities. bank riparian zone) lawns, or crops) have not impacted zone. SCORE ___ (LB) Left Bank 10 9 8 7 6 5 4 3 2 1 0 SCORE ___ (RB) Right Bank 10 9 8 7 6 5 4 3 2 1 0

HABITAT SCORES VALUE OPTIMAL 160 Χ 200 SUB-OPTIMAL 110 Χ 159 MARGINAL 60 Χ 109 POOR < 60

New Jersey AMNET Study — Round 3 Raritan Region

Table 5 - List of AMNET sites with Possible Exceedences:

Amnet# Stream Name Station Location Exceedences AN0192 Rahway R Northfield Ave Tot Phos, TDS AN0193 Rahway R Rt 82 (Morris Ave) nr Washington Ave Tot Phos AN0194 Rahway R Rt 509 Kenilworth Blvd Tot Phos AN0195 Rahway R River Rd & Church St Tot Phos, TSS AN0197 Robinsons Br trib Raritan Rd (Terrell Rd) Otl Shackamaxon Lk Tot Phos AN0198 Robinsons Br trib Lamberts Mill Rd Tot Phos AN0199 Robinsons Br Rt 27 Tot Phos AN0200 Rahway R S Br Parsonage Rd Tot Phos, TDS, PCB, Dioxin AN0201 Rahway R S Br in Merrill Park off Fairview Rd; off of Dover Rd Tot Phos, TDS, PCB, Dioxin AN0202 W Br Elizabeth R Vaux Hall Rd Tot Phos, TDS AN0204 Elizabeth R North Ave Tot Phos, TDS AN0313* Stony Bk Fairview Ave Tot Phos, Temp AN0314 Electric Bk Fairview Ave Tot Phos, Temp AN0315* S Br Raritan R Rt 517 Tot Phos, Temp AN0316* S Br Raritan R off Raritan River Rd (Rt 512) Tot Phos, Temp AN0317* S Br Raritan R River Rd (Ken Lockwood Gorge) Tot Phos, Temp AN0318* Spruce Run Newport Rd Tot Phos AN0320* Willoughby Bk Rt 31 Tot Phos, Temp, pH AN0321 Mulhockaway Ck Van Syckel Rd (Rt 635) Temp AN0323* Beaver Bk Herman Thau Rd Tot Phos AN0324 Beaver Bk Leigh St Tot Phos AN0325* Cakepoulin Ck Lower Lands Down Rd Tot Phos, DDX AN0325B* Cakepoulin Ck Rt 513 Tot Phos, DDX AN0326* S Br Raritan R Stanton Rd pH, Temp AN0328* Assiscong Ck River Rd pH, Temp AN0329* Raritan R S Br Rt 613 (Old York Rd) Tot Phos AN0332* Third Neshanic R Rt 31 DO AN0333 Neshanic R Everitt Rd Tot Phos AN0336* Furmans Bk Welisewitz Rd (below confluence of streams) Tot Phos AN0337 Neshanic R Rt 514 (Amwell Rd.) Tot Phos AN0338* Raritan R S Br Elm St Tot Phos AN0341 Raritan R S Br Studdiford Drive Tot Phos AN0357* Tanners Bk Tanners Brook Rd Temp Tot Phos, Temp AN0358 Lamington R Rt 24 (Cooper Mill Park) Tot Phos, Temp AN0359* Trout Bk State Pk Rd near Hacklebarney St Pk Tot Phos, Temp AN0360* Lamington R Rt 512 Tot Phos, Temp AN0363* Lamington R Rt 523 Tot Phos, Temp AN0367 Rockaway Ck S Br Windy Acres Farm (unpaved farm road) Tot Phos, Temp AN0368 Rockaway Ck S Br Rt 22 Tot Phos, pH AN0369 Rockaway Ck Island Rd Tot Phos, pH AN0370* Lamington R Cowperthwaite Rd Tot Phos AN0371 Chambers Bk B Love Rd Tot Phos AN0374* Raritan R N Br Rt 202 Tot Phos, pH, TSS AN0378 Millstone R Baird Rd Tot Phos, pH, TSS AN0379 Millstone R Rt 33 AN0381 Rocky Bk Main St (outlet of Peddie Lake) Tot Phos, pH AN0382 Millstone R Grovers Mill Rd Tot Phos, pH

* non impaired AMNET site results from the NJDEP 2006 Integrated List

Appendix A — Station Numbers and Locations for the Round 3 Raritan Region AMNET Study

Site Stream Latitude Watershed Site Stream Latitude Watershed Longitude Management Longitude Management Area Area AN0192 Rahway River 40 46'11.022"N 7 AN0329 S Br Raritan River 40 31'01.180"N 8 74 16'59.605"W 74 48'06.911"W AN0193 Rahway River 40 42'28.817"N 7 AN0330 First Neshanic River 40 29'22.982"N 8 74 18'06.441"W 74 51'44.174"W AN0194 Rahway River 40 40'24.010"N 7 AN0331 Second Neshanic River 40 28'59.461"N 8 74 18'46.508"W 74 51'49.423"W AN0195 Rahway River 40 37'05.443"N 7 AN0332 Third Neshanic River 40 28'29.339"N 8 74 16'42.076"W 74 51'46.023"W AN0196 Robinsons Br 40 36'55.135"N 7 AN0333 Neshanic River 40 28'24.360"N 8 74 20'20.659"W 74 49'39.483"W AN0197 UNT to Robinsons Br 40 37'31.702"N 7 AN0334 Back Bk 40 25'46.280"N 8 74 20'49.072"W 74 50'50.907"W AN0198 UNT to Robinsons Br 40 37'29.941"N 7 AN0335 Back Bk 40 27'33.733"N 8 74 19'41.278"W 74 48'22.644"W AN0199 Robinsons Br 40 36'38.305"N 7 AN0336 Furmans Bk 40 27'50.648"N 8 74 17'11.475"W 74 47'09.998"W AN0200 South Br Rahway River 40 33'13.769"N 7 AN0337 Neshanic River 40 29'36.452"N 8 74 20'17.255"W 74 45'11.866"W AN0201 South Br Rahway River 40 34'56.816"N 7 AN0338 S Br Raritan River 40 30'33.765"N 8 74 18'00.962"W 74 43'37.036"W AN0202 West Br Elizabeth River 40 41'33.989"N 7 AN0339 Pleasant Run 40 33'42.941"N 8 74 14'32.817"W 74 47'37.118"W AN0204 Elizabeth River 40 40'39.173"N 7 AN0340 Pleasant Run 40 31'12.166"N 8 74 13'32.202"W 74 44'08.481"W AN0310 S Br Raritan River 40 51'37.094"N 8 AN0341 S Br Raritan River 40 32'48.659"N 8 74 45'35.854"W 74 41'47.348"W AN0311 Drakes Bk 40 51'21.932"N 8 AN0342 Holland Bk 40 34'44.840"N 8 74 40'41.956"W 74 46'33.607"W AN0312 Drakes Bk 40 48'43.555"N 8 AN0343 Holland Bk 40 33'11.473"N 8 74 43'45.689"W 74 42'01.971"W AN0313 Stony Bk 40 48'18.444"N 8 AN0344 UNT to India Bk 40 49'41.598"N 8 74 45'03.008"W 74 36'00.921"W AN0314 Electric Bk 40 47'23.423"N 8 AN0344A India Bk 40 49'42.514"N 8 74 46'34.952"W 74 35'20.931"W AN0315 S Br Raritan River 40 47'06.051"N 8 AN0345 India Bk 40 47'10.332"N 8 74 46 48.068"W 74 37'13.896"W AN0316 S Br Raritan River 40 43 07.160"N 8 AN0346 N Br Raritan River 40 46'16.431"N 8 74 50 30.437"W 74 37'32.833"W AN0317 S Br Raritan River 40 41 48.921"N 8 AN0347 Dawsons Bk 40 48'15.845"N 8 74 52'18.904"W 74 37'41.071"W AN0318 Spruce Run 40 43'29.440"N 8 AN0348 Burnett Bk 40 46'57.039"N 8 74 54'33.994"W 74 38'42.290"W AN0319 Spruce Run 40 41'13.965"N 8 AN0349 Peapack Bk 40 45'16.418"N 8 74 56'02.431"W 74 40'50.428"W AN0320 Willoughby Bk 40 40'17.910"N 8 AN0350 Peapack Bk 40 41'29.592"N 8 74 54'54.388"W 74 38'52.271"W AN0321 Mulhockaway Ck 40 38'50.889"N 8 AN0351 N Br Raritan River 40 40'58.337"N 8 74 58'07.677"W 74 38'18.657"W AN0322 S Br Raritan River 40 38'06.966"N 8 AN0352 Mine Bk 40 42'44.667"N 8 74 54'41.665"W 74 34'45.474"W AN0323 Beaver Bk 40 40'03.182"N 8 AN0353 Mine Bk 40 40'56.332"N 8 74 51'55.159"W 74 37'48.227"W AN0324 Beaver Bk 40 38'10.799"N 8 AN0354 Middle Bk 40 41'37.919"N 8 74 54'34.759"W 74 40'42.730"W AN0325 Cakepoulin Ck 40 36'28.147"N 8 AN0355 Middle Bk 40 38'50.859"N 8 74 54'56.769"W 74 40'51.794"W AN0325B Cakepoulin Ck 40 34'58.880"N 8 AN0356 Lamington River 40 50'06.932"N 8 74 57'30.385"W 74 38'40.546"W AN0326 S Br Raritan River 40 34'20.716"N 8 AN0357 Tanners Bk 40 47'17.943"N 8 74 52'04.310"W 74 43'32.431"W AN0327 Prescott Bk 40 34'24.249"N 8 AN0358 Lamington River 40 46'43.453"N 8 74 51'48.234"W 74 43'18.019"W AN0328 Assiscong Ck 40 32'23.181"N 8 AN0359 Trout Bk 40 45'16.396"N 8 74 50 49.303"W 74 43'55.187"W

Appendix A — Station Numbers and Locations for the Round 3 Raritan Region AMNET Study

Site Stream Latitude Watershed Site Stream Latitude Watershed Longitude Management Longitude Management Area Area AN0360 Lamington River 40 42'56.246"N 8 AN0390 Camp Harmony Br of 40 24'12.202"N 10 74 43'17.630"W Stony Bk 74 48'06.008"W AN0361 UNT to Lamington River 40 42'24.643"N 8 AN0391 Stony Bk 40 22'26.598"N 10 74 42'59.361"W 74 47'37.479"W AN0362 Cold Bk 40 40'30.024"N 8 AN0392 Stony Bk 40 19'52.630"N 10 74 44'16.069"W 74 46'01.800"W AN0363 Lamington River 40 39'38.381"N 8 AN0393 Stony Bk 40 19'59.682"N 10 74 43'44.250"W 74 40'55.949"W AN0364 N Br Rockaway Ck 40 43'31.244"N 8 AN0394 Duck Pond Run 40 18'23.496"N 10 74 47'10.077"W 74 40'04.668"W AN0365 N Br Rockaway Ck 40 41'23.540"N 8 AN0395 Heathcote Bk 40 22'57.502"N 10 74 48'39.928"W 74 34'04.740"W AN0366 N Br Rockaway Ck 40 39'42.387"N 8 AN0396 Heathcote Bk 40 22'11.952"N 10 74 45'57.240"W 74 36'56.978"W AN0367 S Br Rockaway Ck 40 38'22.213"N 8 AN0397 Millstone River 40 22'25.677"N 10 74 48'58.420"W 74 37'12.787"W AN0368 S Br Rockaway Ck 40 37'24.551"N 8 AN0398 Bedens Bk 40 23'03.999"N 10 74 45'59.963"W 74 44'25.474"W AN0369 Rockaway Ck 40 37'23.975"N 8 AN0399 Rock Bk 40 26'23.130"N 10 74 43'15.131"W 74 44'21.646"W AN0370 Lamington River 40 38'04.804"N 8 AN0400 Rock Bk 40 24'46.881"N 10 74 41'12.197"W 74 41'02.493"W AN0371 Chambers(B) Bk 40 37'26.183"N 8 AN0401 Bedens Bk 40 24'52.520"N 10 74 39'46.916"W 74 39 01.533"W AN0372 Chambers(A) Bk 40 36'18.705"N 8 AN0402 40 28'26.673"N 10 74 44'43.402"W 74 39'25.494"W AN0373 Chambers(A) Bk 40 35'32.488"N 8 AN0403 Cruser Bk 40 27'15.949"N 10 74 40'58.840"W 74 39'36.505"W AN0374 N Br Raritan River 40 34'11.002"N 8 AN0404 Back Bk 40 25'57.702"N 10 74 40'41.493"W 74 39'34.796"W AN0375 Dukes Bk 40 33'14.314"N 9 AN0405 Pike Run 40 25'12.408"N 10 74 36'48.227"W 74 38'25.663"W AN0376 Peters Bk 40 34'01.277"N 9 AN0406 Simonson Bk 40 26'18.513"N 10 74 36'18.868"W 74 36'46.689"W AN0377 Raritan River 40 32'39.200"N 9 AN0407 Ten Mile Run 40 27'23.093"N 10 74 34'05.421"W 74 35'08.581"W AN0378 Millstone River 40 14'28.495"N 10 AN0408 Six Mile Run 40 27'18.952"N 10 74 24'04.832"W 74 30'52.166"W AN0379 Millstone River 40 15'43.051"N 10 AN0409 Six Mile Run 40 28'22.389"N 10 74 25'12.305"W 74 34'16.033"W AN0380 Rocky Bk 40 13'38.371"N 10 AN0410 Millstone River 40 28'30.495"N 10 74 26'22.149"W 74 34'34.587"W AN0381 Rocky Bk 40 16'13.026"N 10 AN0411 Royce Bk 40 29'47.714"N 10 74 31'21.855"W 74 38'50.800"W AN0382 Millstone River 40 19'19.653"N 10 AN0412 Royce Bk Br 40 30'40.115"N 10 74 36'28.695"W 74 37'57.560"W AN0382B Millstone River 40 17'27.435"N 10 AN0413 Royce Bk 40 32'13.363"N 10 74 32'58.014"W 74 35'22.668"W AN0382D Millstone River 40 16'28.620"N 10 AN0414 Millstone River 40 32'30.924"N 10 74 28'20.525"W 74 34'07.554"W AN0383 Big Bear Bk 40 16'41.201"N 10 AN0415 Cuckels Bk 40 34'07.355"N 9 74 34'36.982"W 74 34'10.841"W AN0384 Bear Bk 40 19'05.323"N 10 AN0416 W Br Middle Bk 40 36'43.383"N 9 74 36'44.554"W 74 35'25.710"W AN0385 Cranbury Bk 40 18'18.858"N 10 AN0417 W Br Middle Bk 40 35'21.701"N 9 74 28'23.669"W 74 33'48.395"W AN0386 Cranbury Bk 40 19'35.979"N 10 AN0418 E Br Middle Bk 40 36'47.621"N 9 74 36'09.714"W 74 29'47.454"W AN0387 Devils Bk 40 21'42.428"N 10 AN0419 E Br Middle Bk 40 35'29.891"N 9 74 32'42.028"W 74 33'18.072"W AN0388 Shallow Bk 40 20'48.608"N 10 AN0420 Middle Bk 40 34'05.056"N 9 74 33'25.879"W 74 33'12.661"W AN0389 Devils Bk 40 20'35.129"N 10 AN0421 Green Bk 40 38'27.731"N 9 74 35'21.073"W 74 24'49.425"W

Appendix A — Station Numbers and Locations for the Round 3 Raritan Region AMNET Study

Site Stream Latitude Watershed Site Stream Latitude Watershed Longitude Management Longitude Management Area Area AN0422 Stony Bk 40 36'50.903"N 9 AN0452 Iresick Bk 40 23'35.113"N 9 74 26'45.891"W 74 21'33.397"W AN0423 Green Bk 40 36'19.635"N 9 AN0453 Deep Run 40 23'05.771"N 9 74 26'59.268"W 74 18'28.741"W AN0424 Bound Bk 40 34'50.497"N 9 AN0454 Deep Run 40 24'35.749"N 9 74 29'57.414"W 74 20'45.052"W AN0424B Bound Bk 40 33'42.614"N 9 AN0455 Tennent Bk 40 25'40.902"N 9 74 23'51.312"W 74 20'39.356"W AN0425 Ambrose Bk 40 34'03.434"N 9 74 31'12.003"W AN0425A Ambrose Bk 40 32'50.115"N 9 74 27'51.059"W AN0426 Green Bk 40 33'42.746"N 9 74 31'28.350"W AN0427 UNT to Raritan River 40 32'43.133"N 9 74 31'08.009"W AN0428 Raritan River 40 32'27.225"N 9 74 30'45.415"W AN0429 Mile Run 40 30'20.042"N 9 74 28'02.071"W AN0430 Lawrence Bk 40 22'51.506"N 9 74 32'37.700"W AN0431 Lawrence Bk 40 24'58.850"N 9 74 29'36.930"W AN0432 Oakeys Bk 40 25'06.100"N 9 74 29'52.230"W AN0433 Ireland Bk 40 25'13.409"N 9 74 29'05.490"W AN0434 Lawrence Bk 40 26'55.734"N 9 74 26'46.339"W AN0435 Sawmill Bk 40 27'30.816"N 9 74 25'31.092"W AN0436 Mill Bk 40 30'19.531"N 9 74 22'41.572"W AN0437 Manalapan Bk 40 12'03.912"N 9 74 22'37.976"W AN0438 Manalapan Bk 40 15'11.336"N 9 74 20'58.593"W AN0439 Manalapan Bk 40 17'46.133"N 9 74 23'52.302"W AN0440 Manalapan Bk 40 22'29.077"N 9 74 24'55.526"W AN0441 Weamaconk Ck 40 16'16.554"N 9 74 17'39.011"W AN0442 Wemrock Bk 40 15'38.376"N 9 74 18'48.562"W AN0443 Weamaconk Ck 40 17'50.467"N 9 74 21'41.881"W AN0444 McGellairds Bk 40 16'46.999"N 9 74 17'40.200"W AN0445 Tepehemus Bk 40 17'45.840"N 9 74 19'11.045"W AN0446 Milford Bk 40 18'04.840"N 9 74 19'10.375"W AN0447 McGellairds Bk 40 18'06.501"N 9 74 21'24.895"W AN0448 Matchaponix Bk 40 18'51.593"N 9 74 21'42.425"W AN0449 Pine Bk 40 18'55.566"N 9 74 21'00.198"W AN0450 Barclay Bk 40 20'54.059"N 9 74 21'25.188"W AN0451 Matchaponix Bk 40 21'35.558"N 9 74 22'03.691"W

APPENDIX B

Pictures of Morphological Abnormalities in Larval Chironomidae and Amphipoda Recovered in Recent AMNET Surveys

B Gammarus fasciatus with second antennae showing different lengths

Gammarus fasciatus with normal antennae (showing antennal pairs of same length)

All photographs taken by D.Bryson, NJDEP Chironomus species with mentum abnormality

Chironomus species with normal mentum

All photographs taken by D.Bryson, NJDEP Procladius species with abnormal ligula

Procladius species with normal ligula

All photographs taken by D.Bryson, NJDEP Polypedilum species with abnormal mentum

Polypedilum species with normal mentum

All photographs taken by D.Bryson, NJDEP

APPENDIX C

Graphical Comparison of New Jersey Impairment Scores versus Habitat Assessment Scores from the 2004 Raritan Region AMNET Study

C Comparative Scores of HABITAT vs. NJIS Combined Round 3 225

200

175

150

125

100

Habitat Score 75

50

25 R2 = 0.1388 0 0 3 6 9 1215182124273033 NJIS Comparative Scores of HABITAT vs. NJIS WMA 7 Round 3 225 200 175 150 125 100

Habitat Score 75 50 25 R2 = 0.2622 0 036912151821242730 NJIS Comparative Scores of HABITAT vs. NJIS WMA 8 Round 3 225 200 175 150 125 100 75 Habitat Score 50 25 R2 = 0.1268 0 0 3 6 9 12 15 18 21 24 27 30 33 NJIS Comparative Scores of HABITAT vs. NJIS WMA 9 Round 3 225 200 175 150 125 100 75 Habitat Score 50 25 2 0 R = 0.1301 0 3 6 9 12151821242730 NJIS Comparative Scores of HABITAT vs. NJIS WMA 10 Round 3 225 200 175 150 125 100 75 Habitat Score 50 25 R2 = 0.0201 0 03691215182124273033 NJIS

APPENDIX D

Taxonomic and Statistical Data, NJIS Scores, Habitat Assessment Scores and Observations from the 2004 Raritan Region AMNET Study (Site numbers, locations and USGS topographic quadrangle, top of page.)

Notes/Definitions:

Statistical data includes those biometric results that are applied to the NJIS rating. Appendix D also includes certain biometrics that have been given as optional for the RBP analysis [2] but are not employed for the NJIS rating [12]; these include ratios of certain functional types or pollution sensitive to pollution tolerant groups; for these (1-3 below), higher values generally indicate better water quality.

1. Scraper/Filtering Collector Ratio — dominance of filtering collectors indicates organic enrichment; however, if toxicants are present in the system, their adsorption on macrophytes and fine particulate organics can affect the abundance of filtering collectors. 2. Shredder/Total Ratio — considering their diet of coarse particulate organic matter (CPOM), a lack of shredders may indicate the presence of toxicants, particularly from terrestrial sources (e.g. pesticides), as these are readily adsorbed to the CPOM. 3. EPT/Chironomid Ratio — even distribution among the major groups, with strong representation in the pollution-sensitive taxa (Ephemeroptera, Plecoptera, Trichoptera), reflects a good biotic condition; dominance of chironomids reflects environmental stress.

Included in the NJIS score are: 1. Taxa Richness – number of families represented in sample. 2. Family Biotic Index – assigns a pollution tolerance level to each family on a scale of zero to ten, zero being least tolerant. 3. Dominant Family – expressed as a percent of total families. 4. Number of EPT families – E + P + T. 5. Percent EPT - % of total families. See METHODS, Table 1.

Other notes: 1. UNT – un-named tributary 2. Blood Red Chironomidae – primarily members of the tribe Chironomini (subfamily Chironominae), which posses a hemoglobin-like pigment that retains oxygen, thus increasing their tolerance to organic pollution. 3. Habitat observations supplement the habitat assessment scores in Table 2 and Appendix C; Open Canopy = overhead vegetation; water quality measurements taken in field include temperature (°C), pH, dissolved oxygen, conductivity.

D

APPENDIX D (cont.) Taxonomic List of Macroinvertebrate Families Found at New Jersey AMNET Sites

Phylum PLATYHELMINTHES Class TURBELLARIA (flatworms) Order AMPHIPODA (scuds, sideswimmers) Order TRICLADIDA Family Gammaridae Family Dendrocoelidae Talitridae Planariidae Order DECAPODA (crayfish, shrimp) Order MACROSTOMIDA Family Astacidae Family Macrostomidae Cambaridae Order NEORHABDOCOELA Palaemonidae Family Typhloplanidae Class ARACHNOIDEA Order ALLOEOCOELA Order HYDRACARINA (water mites) Family Plagiostomidae Family Arrenuridae Prorhynchidae Axonopsidae Hydryphantidae Phylum NEMERTEA (proboscis worms) Hygrobatidae Class ENOPLA Lebertiidae Order HOPLONEMERTINI Limnesiidae Family Tetrastemmatidae Pionidae Sperchonidae Phylum NEMATODA (roundworms) Unionicolidae Class CHILOPODA (centipedes) Phylum ANNELIDA Class DIPLOPODA (millipedes) Class OLIGOCHAETA (aquatic earthworms) Class INSECTA Order HAPLOTAXIDA Order COLLEMBOLA (springtails) Family Aeolosomatidae Family Entomobryidae Enchytraeidae Hypogastruridae Haplotaxidae Isotomidae Lumbricidae Onychiuridae Naididae Poduridae Tubificidae Order PLECOPTERA (stoneflies) Order LUMBRICULIDA Family Capniidae Family Lumbriculidae Chloroperlidae Class BRANCHIOBDELLIDA Leuctridae Family Branchiobdellidae Nemouridae Class POLYCHAETA Peltoperlidae Family Sabellidae Perlidae Class HIRUDINEA (leeches) Perlodidae Order RHYNCHOBELLIDA Pteronarcyidae Family Glossiphoniidae Taeniopterygidae Piscicolidae Order EPHEMEROPTERA (mayflies) Order ARHYNCHOBDELLIDA Family Baetidae Family Erpobdellidae Baetiscidae Order GNATHOBDELLIDA Caenidae Family Hirudinidae Ephemerellidae Ephemeridae Phylum ARTHROPODA Heptageniidae Class CRUSTACEA Leptophlebiidae Order ISOPODA (aquatic sow bugs) Metretopodidae Family Asellidae Oligoneuriidae Oniscidae Polymitarcyidae Porcellionidae Potamanthidae Siphlonuridae Tricorythidae

Includes only those taxa that are employed in calculation of the NJIS rating; major taxa are listed in the order presented in Pennak (1978) [17].

Order ODONATA Order DIPTERA (flies, midges) Suborder ANISOPTERA (dragonflies) Family Athericidae Family Aeshnidae Blephariceridae Cordulegastridae Ceratopogonidae Corduliidae Chaoboridae Gomphidae Chironomidae Libellulidae Culicidae Macromiidae Dixidae Suborder ZYGOPTERA (damselflies) Dolichopodidae Family Calopterygidae Empididae Coenagrionidae Ephydridae Lestidae Muscidae Order HEMIPTERA (true bugs) Phoridae Family Belostomatidae Psychodidae Corixidae Ptychopteridae Gerridae Sciomyzidae Mesoveliidae Simuliidae Nepidae Stratiomyidae Notonectidae Syrphidae Pleidae Tabanidae Veliidae Tanyderidae Order MEGALOPTERA Tipulidae Family Corydalidae (dobsonflies, fishflies) Phylum MOLLUSCA Sialidae (alderflies) Class GASTROPODA (snails) Order NEUROPTERA Order BASOMMATOPHORA Family Sisyridae (spongilla flies) Family Ancylidae Order TRICHOPTERA (caddisflies) Lymnaeidae Family Brachycentridae Physidae Calamoceratidae Planorbidae Glossosomatidae Order MESOGASTROPODA Helicopsychidae Family Hydrobiidae Hydropsychidae Pleuroceridae Hydroptilidae Valvatidae Lepidostomatidae Viviparidae Leptoceridae Class PELECYPODA (clams, mussels) Limnephilidae Order EULAMELLIBRANCHIA Molannidae Family Unionidae Odontoceridae Order HETERODONTA Philopotamidae Family Corbiculidae Phryganeidae Sphaeriidae Polycentropodidae Psychomyiidae Rhyacophilidae Sericostomatidae Order LEPIDOPTERA (aquatic caterpillars) Family Nepticulidae Pyralidae Order COLEOPTERA (beetles) Family Chrysomelidae Curculionidae Dryopidae Dytiscidae Elmidae Gyrinidae Haliplidae Hydrophilidae Lampyridae Noteridae Psephenidae Ptilodactylidae Scirtidae

Station: AN0192 Rahway River, Northfield Ave, West Orange, Essex County Caldwell USGS Quadrangle Date Sampled: 10/13/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Tubificidae 10 38 BloodRed Chironomidae 8 20 Naididae 7 16 Coenagrionidae 9 8 Sphaeriidae 8 6 Chironomidae 6 3 Physidae 7 3 Planorbidae 6 3 Veliidae 9 2 Aeshnidae 3 1 Corduliidae 5 1 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 101 % Contribution of Dominant Family: 37.62 % ( Tubificidae ) Family Biotic Index: 8.47 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 75 ( Marginal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 30.0' / 3.0' Substrate: Gravel, Mud, Silt....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Poor Canopy: Mostly Closed....Other: Water Temp. 12.6 C / pH 7.0 SU / DO 8.2 mg/L / Cond. 1130 umhos; Suburban Sample site runs through parking lot of medical building. ; ------

Station: AN0193 Rahway River, Route 82, Springfield Twp., Union County Roselle USGS Quadrangle Date Sampled: 10/13/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 43 Asellidae 8 13 Gammaridae 4 10 Coenagrionidae 9 9 Chironomidae 6 3 BloodRed Chironomidae 8 3 Planariidae 4 3 Glossiphoniidae 8 3 Sphaeriidae 8 3 Tubificidae 10 2 Lumbriculidae 8 2 Calopterygidae 5 1 Libellulidae 9 1 Empididae 6 1 Hydroptilidae 4 1 Naididae 7 1 Elmidae 4 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 100 % Contribution of Dominant Family: 43.00 % ( Hydropsychidae ) Family Biotic Index: 5.70 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 44.00 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 152 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 63.0' / 2' Substrate: Gravel, Snags....StreamBank Vegetation/Stability: Trees, Shrubs/Fair Canopy: Partly Open....Other: Water Temp. 12.6 C / pH 7.1 SU / DO 8.0 mg/L / Cond. 1360 umhos; Suburban. Storm sewers present.; Ducks, periphyton present. ------

Station: AN0194 Rahway River, Route 509, Cranford Twp., Union County Roselle USGS Quadrangle Date Sampled: 10/13/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 49 Tubificidae 10 25 Hydrobiidae 8 12 BloodRed Chironomidae 8 4 Naididae 7 3 Chironomidae 6 3 Asellidae 8 2 Hydropsychidae 4 1 Coenagrionidae 9 1 Sphaeriidae 8 1 Glossiphoniidae 8 1 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 102 % Contribution of Dominant Family: 48.04 % ( Gammaridae ) Family Biotic Index: 6.45 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 0.98 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 120 ( Sub-Optimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 70.0' / 2.5' - 3.5' Substrate: Sand, Silt....StreamBank Vegetation/Stability: Trees, Shrubs/Fair Canopy: Mostly Open....Other: Water temp. 13.4 C / pH 7.4 SU / DO 7.8 mg/L / Cond. 740 umhos; Suburban. Storm sewers present. Station downstream of dam. USGS Station present.; Ducks, periphyton present. ------

Station: AN0195 Rahway River, River Rd. & Church St., Rahway, Union County Perth Amboy USGS Quadrangle Date Sampled: 10/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 52 Elmidae 4 24 Planariidae 4 9 Hydropsychidae 4 8 Sphaeriidae 8 5 Ancylidae 6 2 Chironomidae 6 2 Planorbidae 6 2 Tetrastemmatidae 7 2 Heptageniidae 4 1 Hydroptilidae 4 1 Erpobdellidae 8 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 109 % Contribution of Dominant Family: 47.71 % ( Gammaridae ) Family Biotic Index: 4.39 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 9.17 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 134 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 82.5' / 1.0' Substrate: Cobbles, Gravel....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Fair Canopy: Open....Other: Water Temp. 11.5 C / pH 8.1 SU / DO 8.8 mg/L / Cond. 491 umhos; Suburban. Storm sewers present.; ------

Station: AN0196 Robinsons Brook, Goodmans Crossing, Scotch Plains Twp., Union County Perth Amboy USGS Quadrangle Date Sampled: 10/05/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydrobiidae 8 63 Gammaridae 4 11 Tubificidae 10 9 Asellidae 8 6 Sphaeriidae 8 5 Plagiostomidae 4 4 Chironomidae 6 3 Coenagrionidae 9 2 Planariidae 4 1 Elmidae 4 1 Naididae 7 1 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 106 % Contribution of Dominant Family: 59.43 % ( Hydrobiidae ) Family Biotic Index: 7.48 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 101 ( Marginal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear, Grayish Haze....Flow: Slow....Width/Depth (ft): 28.0' / 2.5' Substrate: Gravel, Mud, Silt....StreamBank Vegetation/Stability: Trees, shrubs, Grass/Poor Canopy: Closed....Other: Water Temp. 13.5 C / pH 6.9 SU / DO 6.3 mg/L / Cond. 566 umhos; Suburban. Storm sewers present. Fish, periphyton, macrophytes, debris present.; Construction on left. Upstream of bridge, flow is very slow. ------

Station: AN0197 UNT To Robinsons Brook, Raritan Rd., Scotch Plains Twp., Union County Roselle USGS Quadrangle Date Sampled: 10/07/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 86 Chironomidae 6 4 Planariidae 4 3 BloodRed Chironomidae 8 3 Coenagrionidae 9 2 Elmidae 4 2 Asellidae 8 1 Empididae 6 1 Naididae 7 1 Nematoda 6 1 Sphaeriidae 8 1 Planorbidae 6 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 107 % Contribution of Dominant Family: 80.37 % ( Hydropsychidae ) Family Biotic Index: 4.47 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 80.37 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 125 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Hydropsychidae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 23.0' / <1.0' Substrate: Cobbles, Gravel/Sand....StreamBank Vegetation/Stability: Grasses, Shrubs, Trees/Fair Canopy: Mostly Open....Other: Water Temp. 15.0 C / pH 7.7 SU / DO 7.0 mg/L / Cond. 372 umhos; Suburban. Storm sewers present.; Some algae growth, Filamentous algae, and fish present. ------

Station: AN0198 UNT To Robinsons Brook, Lamberts Mill Rd., Westfield Twp., Union County Perth Amboy USGS Quadrangle Date Sampled: 10/07/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydrobiidae 8 26 Coenagrionidae 9 23 Gammaridae 4 15 Planorbidae 6 9 Tubificidae 10 8 Naididae 7 7 BloodRed Chironomidae 8 5 Chironomidae 6 3 Sphaeriidae 8 2 Aeshnidae 3 1 Glossiphoniidae 8 1 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 100 % Contribution of Dominant Family: 26.00 % ( Hydrobiidae ) Family Biotic Index: 7.43 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 111 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 22.0' / <1.0' Substrate: Gravel/Sand, Silt....StreamBank Vegetation/Stability: Shrubs, Trees/Fair Canopy: Partly Open....Other: Water Temp. 13.9 C / pH 7.5 SU / DO 6.9 mg/L / Cond. 373 umhos; Suburban. High school on left bank. Storm sewers present.; Fish, periphyton, frogs, filamentous algae present. ------

Station: AN0199 Robinsons Brook, Route 27, Rahway, Union County Perth Amboy USGS Quadrangle Date Sampled: 10/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 46 Hydropsychidae 4 25 Elmidae 4 12 Coenagrionidae 9 8 Asellidae 8 4 BloodRed Chironomidae 8 2 Sphaeriidae 8 2 Tipulidae 3 1 Lumbricidae 10 1 Planorbidae 6 1 ------Statistical Analysis ------Number of Taxa: 10 Total Number of Individuals: 102 % Contribution of Dominant Family: 45.10 % ( Gammaridae ) Family Biotic Index: 4.77 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 24.51 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 116 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 45.0' / 1.0' Substrate: Gravel / sand....StreamBank Vegetation/Stability: Trees, Shrubs/Fair Canopy: Mostly Closed....Other: Water temp. 12.2 C / pH 7.5 SU / DO 9.3 mg/L / Cond. 245 umhos; Suburban Storm sewers present; ------

Station: AN0200 South Branch Rahway River, Parsonage Rd., Edison Twp., Middlesex County Perth Amboy USGS Quadrangle Date Sampled: 10/07/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 65 Planariidae 4 8 Chironomidae 6 8 Erpobdellidae 8 7 Elmidae 4 7 Tubificidae 10 2 Tetrastemmatidae 7 2 Hydrophilidae 5 1 Asellidae 8 1 Gammaridae 4 1 Empididae 6 1 Naididae 7 1 Planorbidae 6 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 105 % Contribution of Dominant Family: 61.90 % ( Hydropsychidae ) Family Biotic Index: 4.70 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 61.90 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 113 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Hydropsychidae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 15.0' / <0.5' Substrate: Cobbles, Gravel/Sand....StreamBank Vegetation/Stability: Grasses, Few Shrubs/Fair Canopy: Open....Other: Water Temp. 19.0 C / pH 8.2 SU / DO 7.2 mg/L / Cond. 268 umhos; Suburban. Storm sewers present. Station downstream of outlet of lake. Menlo Park upstream.; Fish present. ------

Station: AN0201 South Branch Rahway River, Merrill Park, Woodbridge Twp., Middlesex County Perth Amboy USGS Quadrangle Date Sampled: 10/05/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 66 Gammaridae 4 5 Tipulidae 3 4 BloodRed Chironomidae 8 4 Naididae 7 4 Chironomidae 6 3 Physidae 7 3 Tubificidae 10 2 Lumbriculidae 8 2 Sphaeriidae 8 2 Baetidae 4 1 Cambaridae 5 1 Dytiscidae 5 1 Simuliidae 6 1 Elmidae 4 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 66.00 % ( Hydropsychidae ) Family Biotic Index: 4.71 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 67.00 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 131 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Hydropsychidae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 35.0' / <1.0' - 1.5' Substrate: Gravel....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Poor Canopy: Open....Other: Water temp. 14.2 C / pH 6.5 SU / DO 7.1 mg/L / Cond. 714 umhos; Suburban Periphyton present; ------

Station: AN0202 West Br Elizabeth River, Vaux Hall Rd, Union Twp, Union County Elizabeth USGS Quadrangle Date Sampled: 10/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------

Not Sampled due to bridge construction work

Station: AN0204 Elizabeth River, North Ave., Union Twp., Union County Elizabeth USGS Quadrangle Date Sampled: 10/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 39 Tubificidae 10 38 Chironomidae 6 8 Planariidae 4 5 Tipulidae 3 2 Baetidae 4 1 Enchytraeidae 10 1 Naididae 7 1 Sphaeriidae 8 1 BloodRed Chironomidae 8 1 Tetrastemmatidae 7 1 Simuliidae 6 1 Elmidae 4 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 100 % Contribution of Dominant Family: 39.00 % ( Hydropsychidae ) Family Biotic Index: 6.64 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 40.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 102 ( Marginal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 42.0' / 2.0' Substrate: Cobbles, Gravel/Sand....StreamBank Vegetation/Stability: Shrubs, Grass, Trees/Poor Canopy: Open....Other: Water Temp. 12.0 C /pH 7.7 SU / DO 8.6 mg/L / Cond. 655 umhos; Urban. Storm sewers present.; Some trash present, fish present. ------

Station: AN0310 S. Br Raritan River, Smithtown Rd Near Outl Of Budd Lake, Morris County Hackettstown USGS Quadrangle Date Sampled: 04/06/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Asellidae 8 16 Hydrobiidae 8 15 Planariidae 4 11 Naididae 7 11 Sphaeriidae 8 10 Gammaridae 4 7 Hydropsychidae 4 6 Plagiostomidae 4 6 Caenidae 7 4 Planorbidae 6 4 BloodRed Chironomidae 8 3 Empididae 6 3 Chironomidae 6 3 Ancylidae 6 2 Physidae 7 2 Corydalidae 0 1 Tubificidae 10 1 Valvatidae 4 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 106 % Contribution of Dominant Family: 15.09 % ( Asellidae ) Family Biotic Index: 6.39 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 9.43 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 178 ( Optimal ) EPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 15 ' / 1.2 ' Substrate: Cobbles....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Open....Other: Water temp. 7.3 C /pH 7.7 SU /DO 10.6 mg/L /Cond 278 umhos; Suburban; small dam at upstream lake filamentous algae; USGS gage station; ------

Station: AN0311 Drakes Brook , Emmans Road, Roxbury Twp., Morris County Chester USGS Quadrangle Date Sampled: 4/6/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 33 Elmidae 4 23 Hydropsychidae 4 17 Gammaridae 4 7 Simuliidae 6 7 Empididae 6 6 Philopotamidae 3 3 Nemouridae 2 2 Corydalidae 0 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 10 Total Number of Individuals: 100 % Contribution of Dominant Family: 33.00 % ( Chironomidae ) Family Biotic Index: 4.80 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 22.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 124 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 26.5' / 1-2' Substrate: Cobbles, gravel, sand....StreamBank Vegetation/Stability: Grasses, shrubs, trees/Fair Canopy: Mostly Open....Other: Water temp. 6.4C / pH 7.5 SU / DO 10.4 mg/L / Cond. 520umhos; Rural, forested Pond next to stream. Small weir under small foot bridge. ; Flood lamp wired to right bank below bridge. ------

Station: AN0312 Drakes Brook, Bartley Road, Washington Twp., Morris County Chester USGS Quadrangle Date Sampled: 4/6/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 53 Naididae 7 9 Philopotamidae 3 9 Empididae 6 7 BloodRed Chironomidae 8 7 Elmidae 4 6 Hydropsychidae 4 3 Planariidae 4 3 Psephenidae 4 3 Limnephilidae 4 2 Heptageniidae 4 2 Baetidae 4 1 Tipulidae 3 1 Coenagrionidae 9 1 Asellidae 8 1 Brachycentridae 1 1 ------Statistical Analysis ------Number of Taxa: 16 Total Number of Individuals: 109 % Contribution of Dominant Family: 48.62 % ( Chironomidae ) Family Biotic Index: 5.57 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 6 % EPT: 16.51 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 153 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 40.0 / <1.0-1.5 Substrate: Cobbles, Gravel/Sand....StreamBank Vegetation/Stability: Grasses, Trees, Shrubs/Poor Canopy: Partly Open....Other: Suburban, forested; Water temp. 5.8C / pH 7.4 SU / DO 11.8 mg/L / Cond. 425 umhos Downstream banks scoured and eroded. No riffles downstream - ; siltation evident. Site downstream of high school parking lot. ------

Station: AN0313 Stony Brook, Fairview Ave., Washington Twp., Morris County Hackettstown USGS Quadrangle Date Sampled: 4/6/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 27 Heptageniidae 4 21 Simuliidae 6 17 Baetidae 4 13 Perlidae 1 3 Empididae 6 3 Taeniopterygidae 2 3 Siphlonuridae 7 2 Nemouridae 2 2 Hydropsychidae 4 2 Blephariceridae 0 1 Astacidae 7 1 Tabanidae 6 1 Philopotamidae 3 1 Enchytraeidae 10 1 Chloroperlidae 1 1 Lumbriculidae 8 1 Sphaeriidae 8 1 Limnephilidae 4 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 102 % Contribution of Dominant Family: 26.47 % ( Chironomidae ) Family Biotic Index: 4.90 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 10 % EPT: 48.04 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 164 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 20' / <1 -1.0' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Trees, shrubs/Fair Canopy: Partly Open....Other: Suburban, Forested; Water temp. 3.7 C / pH 7.9 SU / DO 13.7 mg/L / Cond. 159 umhos Sampled at larger of two streams.; ------

Station: AN0314 Electric Brook, Fairview Ave, Washington Twp., Morris County Hackettstown USGS Quadrangle Date Sampled: 04/06/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 80 Ephemerellidae 1 10 Empididae 6 2 Lumbriculidae 8 2 Naididae 7 2 Tabanidae 6 1 Hydropsychidae 4 1 Dytiscidae 5 1 Leuctridae 0 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 10 Total Number of Individuals: 101 % Contribution of Dominant Family: 79.21 % ( Chironomidae ) Family Biotic Index: 5.45 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 11.88 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 101 ( Marginal ) EPA Protocol Deficiency(s) noted: Chironomidae Family Overwhelmingly Dominant ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 19.5 ' / < 1.0 - 1.0' Substrate: Gravel/sand, silt....StreamBank Vegetation/Stability: Trees, grass/Poor Canopy: Partly Open....Other: Water temp. 11.0 C /pH 7.3 SU /DO 7.5 mg/L /Cond 384 umhos; Suburban; outfall pipe flowing stream intermittent, dry upstream; very slow flow; small weir & footbridge downstream ------

Station: AN0315 S. Br Raritan River, Rt 517, Washington Twp, Morris County Hackettstown USGS Quadrangle Date Sampled: 04/06/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Ephemerellidae 1 25 Chironomidae 6 24 Hydropsychidae 4 11 Tubificidae 10 10 Lumbriculidae 10 7 Simuliidae 6 5 Limnephilidae 4 4 Elmidae 4 4 Empididae 6 3 Tipulidae 3 2 Enchytraeidae 10 2 Heptageniidae 4 1 Erpobdellidae 8 1 BloodRed Chironomidae 8 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 100 % Contribution of Dominant Family: 25.00 % ( Ephemerellidae ) Family Biotic Index: 5.09 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 41.00 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 147 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 50 ' / <1.0 ' - 1 ' Substrate: Cobbles....StreamBank Vegetation/Stability: Trees, grass/Poor Canopy: Open....Other: Water temp. 5.2 C /pH 7.3 SU /DO 11.5 mg/L /Cond 297 umhos; Suburban / forested auto repair shop adjacent to stream; historic building on left bank ------

Station: AN0316 S Br Raritan River, Rt 512 Off Raritan River Rd., Califon Boro, Hunterdon County Califon USGS Quadrangle Date Sampled: 4/7/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 39 Tubificidae 10 9 Simuliidae 6 9 Ephemerellidae 1 8 Hydropsychidae 4 5 Gammaridae 4 5 Plagiostomidae 4 4 Limnephilidae 4 4 Leptoceridae 4 3 Naididae 7 3 Nemouridae 2 2 Aeshnidae 3 2 Elmidae 4 2 Sphaeriidae 8 1 Planorbidae 6 1 BloodRed Chironomidae 8 1 Psychomyiidae 2 1 Gomphidae 1 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 100 % Contribution of Dominant Family: 39.00 % ( Chironomidae ) Family Biotic Index: 5.34 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 6 % EPT: 23.00 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 178 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 79.0' / 1-2' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Mostly Open....Other: Suburban and forested; Water temp. 10.0 C / pH 7.3 SU / DO 11.0 mg/L / Cond. 309 umhos Geese observed. Trout stocked.; ------

Station: AN0317 S. Br. Raritan River, River Rd (Ken Lockwood Gorge), Lebanon Twp, Hunterdon County Califon USGS Quadrangle Date Sampled: 04/27/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 27 Baetidae 4 20 Ephemerellidae 1 18 Lumbriculidae 8 11 Simuliidae 6 6 Hydropsychidae 4 3 Siphlonuridae 7 3 Perlidae 1 2 Elmidae 4 2 Naididae 7 2 Psephenidae 4 2 Nemouridae 2 1 Gammaridae 4 1 Pleuroceridae 6 1 Tubificidae 10 1 BloodRed Chironomidae 8 1 Sphaeriidae 8 1 Limnephilidae 4 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 104 % Contribution of Dominant Family: 25.96 % ( Chironomidae ) Family Biotic Index: 4.76 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 8 % EPT: 47.12 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 183 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 50 ' / 2- 3 ' Substrate: Cobbles....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Mostly Open....Other: Water temp. 12.9 C /pH 7.3 SU /DO10.9 mg/L /Cond 215 umhos; Forested (Ken Lockwood Gorge) fish; ------

Station: AN0318 Spruce Run, Newport Rd. ; Lebanon Twp., Hunterton County High Bridge USGS Quadrangle Date Sampled: 4/7/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Simuliidae 6 20 Nemouridae 2 19 Baetidae 4 17 Ephemerellidae 1 17 Lumbriculidae 8 6 Heptageniidae 4 6 Hydropsychidae 4 3 Rhyacophilidae 0 3 Aeshnidae 3 1 Chironomidae 6 1 Dytiscidae 5 1 Gomphidae 1 1 Calopterygidae 5 1 Oligoneuriidae 2 1 Elmidae 4 1 Brachycentridae 1 1 Psephenidae 4 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 100 % Contribution of Dominant Family: 20.00 % ( Simuliidae ) Family Biotic Index: 3.58 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 8 % EPT: 67.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 178 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 20' / <1.0 - 1' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Trees, shrubs, grass/Good Canopy: Partly Open....Other: Rural, Forested; Water temp. 7.9 C / pH 7.6 SU / DO 12.0 mg/L / Cond. 140 umhos Filamentous algae. Small water fall flowing into stream down stream.; Minnows. ------

Station: AN0319 Spruce Run, Route 31; Glen Gardner Boro, Hunterdon County High Bridge USGS Quadrangle Date Sampled: 4/7/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 59 Ephemerellidae 1 11 Tubificidae 10 11 Simuliidae 6 4 Nemouridae 2 3 Elmidae 4 3 Gammaridae 4 3 Baetidae 4 2 Hydropsychidae 4 2 Oligoneuriidae 2 2 Siphlonuridae 7 1 Ptilodactylidae 1 1 Empididae 6 1 Leptophlebiidae 2 1 Lumbriculidae 8 1 BloodRed Chironomidae 8 1 Rhyacophilidae 0 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 108 % Contribution of Dominant Family: 54.63 % ( Chironomidae ) Family Biotic Index: 5.42 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 9 % EPT: 22.22 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 162 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 26.0 / 1.0-1.5 ' Substrate: Cobbles, gravel, silt....StreamBank Vegetation/Stability: Shrubs, trees/Fair Canopy: Mostly Open....Other: Rural ; Water temp 8.1C / pH 7.4 SU/ DO 13.1 mg/L / Cond. 176 umhos Filamentous algae; wood piles adjacent to stream (private cutting operation); Some siltation; rock wall stabilizing left and right banks for short distance ------

Station: AN0320 Willoughby Brook, Rt 31, Clinton Twp., Hunterdon County High Bridge USGS Quadrangle Date Sampled: 4/7/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Heptageniidae 4 22 Chironomidae 6 20 Baetidae 4 18 Limnephilidae 4 14 Gammaridae 4 5 Ephemerellidae 1 4 Rhyacophilidae 0 3 Simuliidae 6 3 Tubificidae 10 2 Elmidae 4 2 Taeniopterygidae 2 2 Perlidae 1 1 Hydropsychidae 4 1 Enchytraeidae 10 1 Nemouridae 2 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 99 % Contribution of Dominant Family: 22.22 % ( Heptageniidae ) Family Biotic Index: 4.31 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 9 % EPT: 66.67 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 174 ( Optimal ) EPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 23' / <1 - 1.5' Substrate: Cobbles, gravel, silt, snags....StreamBank Vegetation/Stability: Trees, shrubs, and grass/Good Canopy: Partly Open....Other: Suburban; Water temp. 7.5 C, pH 7.4 SU, DO 10.4 mg/L , Cond. 179 umhos Next to autobody repair shop. Near Spruce run reservoir; Some siltation near bridge. ------

Station: AN0321 Mulhockaway Creek, Van Syckel Rd (Rt 635), Union Twp, Hunterdon County High Bridge USGS Quadrangle Date Sampled: 04/22/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Lumbriculidae 8 53 Ephemerellidae 1 12 Baetidae 4 8 Chironomidae 6 7 Tipulidae 3 3 Empididae 6 3 Psephenidae 4 3 Heptageniidae 4 3 Hydropsychidae 4 2 Naididae 7 2 Limnephilidae 4 1 Rhyacophilidae 0 1 Elmidae 4 1 Gomphidae 1 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 100 % Contribution of Dominant Family: 53.00 % ( Lumbriculidae ) Family Biotic Index: 5.92 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 6 % EPT: 27.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 177 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 28 ' / <1.0 - 1 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, vines/Good Canopy: Mostly Open....Other: Water temp. 17.0 C /pH 9.0 SU /DO 11.8 mg/L /Cond 183 umhos; Forested. USGS gage station trout stocked stream; filamentous algae ------

Station: AN0322 S. Br Raritan River, Rt 173 (Cr 513), Clinton, Hunterdon County High Bridge USGS Quadrangle Date Sampled: 04/22/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 37 Elmidae 4 21 Ephemerellidae 1 19 Heptageniidae 4 8 Empididae 6 5 Corbiculidae 8 3 Hydropsychidae 4 2 Naididae 7 2 Perlidae 1 1 Tipulidae 3 1 Planariidae 4 1 Oligoneuriidae 2 1 Lepidostomatidae 1 1 Tubificidae 10 1 Lumbriculidae 8 1 Nematoda 6 1 Pyralidae 5 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 107 % Contribution of Dominant Family: 34.58 % ( Chironomidae ) Family Biotic Index: 4.49 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 6 % EPT: 29.91 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 111 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 115 ' / 1 - 2 ' Substrate: Cobbles, gravel/sand, silt....StreamBank Vegetation/Stability: Trees, weeds/Fair Canopy: Open....Other: Water temp. 14.9 C /pH 8.1 SU /DO 11.3 mg/L /Cond 264 umhos; Urban; site downstream of a dam ducks and filamentous algae; ------

Station: AN0323 Beaver Brook, Herman Thau Rd, Clinton Twp, Hunterdon County Califon USGS Quadrangle Date Sampled: 04/27/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Ephemerellidae 1 54 Nemouridae 2 12 Chironomidae 6 11 Baetidae 4 8 Limnephilidae 4 7 Enchytraeidae 10 3 Tipulidae 3 2 Leptophlebiidae 2 2 BloodRed Chironomidae 8 2 Gomphidae 1 1 Rhyacophilidae 0 1 Peltoperlidae 1 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 104 % Contribution of Dominant Family: 51.92 % ( Ephemerellidae ) Family Biotic Index: 2.52 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 7 % EPT: 81.73 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 184 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 17 ' / 1 - 2 ' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Trees, grass, shrubs/Good Canopy: Partly Open....Other: Water temp. 11.1 C /pH 7.4 SU /DO 11.8 mg/L /Cond 160 umhos; Rural / forested salamander and filamentous algae observed; ------

Station: AN0324 Beaver Brook, Lehigh St, Clinton, Hunterdon County High Bridge USGS Quadrangle Date Sampled: 04/22/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 75 Lumbriculidae 8 5 Naididae 7 3 Tipulidae 3 2 Baetidae 4 2 Empididae 6 2 Gammaridae 4 2 Heptageniidae 4 2 Asellidae 8 1 Philopotamidae 3 1 Ephemerellidae 1 1 Lepidostomatidae 1 1 Tetrastemmatidae 7 1 Simuliidae 6 1 Elmidae 4 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 75.00 % ( Chironomidae ) Family Biotic Index: 5.83 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 7.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 103 ( Marginal ) USEPA Protocol Deficiency(s) noted: Chironomidae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 23 ' / <1.0 - 1 ' Substrate: Gravel/sand, silt....StreamBank Vegetation/Stability: Trees, shrubs, vines/Poor Canopy: Mostly Closed....Other: Water temp. 16.2 C /pH 9.4 SU /DO 13.9 mg/L /Cond 434 umhos; Urban. rock rip-rap on both banks; ------

Station: AN0324A Sidney Brook, Rt 617 (Sidney Rd), Franklin Twp, Hunterdon County Pittstown USGS Quadrangle Date Sampled: 04/29/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Ephemerellidae 1 22 Elmidae 4 14 Chironomidae 6 13 Psephenidae 4 10 Hydropsychidae 4 9 Heptageniidae 4 9 Baetidae 4 4 Empididae 6 3 Tetrastemmatidae 7 3 Glossosomatidae 0 2 Hydroptilidae 4 2 Naididae 7 2 Nemouridae 2 1 Coenagrionidae 9 1 Philopotamidae 3 1 Gomphidae 1 1 Lepidostomatidae 1 1 Lumbriculidae 8 1 Limnephilidae 4 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 100 % Contribution of Dominant Family: 22.00 % ( Ephemerellidae ) Family Biotic Index: 3.73 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 10 % EPT: 52.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 159 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 10-12 ' / 1-2 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Partly Open....Other: Water temp. 13.3 C /pH 7.3 SU /DO 10.7 mg/L /Cond 233 umhos; Rural / forested. Horses in a pen on right bank deep and slow at bridge; right bank mowed and cleared; small lastic pipe on right bank upstream ------

Station: AN0325 Cakepoulin Creek, Lower Landsdown Rd, Franklin Twp, Hunterdon County Pittstown USGS Quadrangle Date Sampled: 04/29/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Ephemerellidae 1 43 Heptageniidae 4 14 Lumbriculidae 8 12 Baetidae 4 9 BloodRed Chironomidae 8 7 Oligoneuriidae 2 4 Lepidostomatidae 1 4 Hydropsychidae 4 2 Gomphidae 1 2 Psephenidae 4 2 Perlidae 1 1 Tipulidae 3 1 Corydalidae 0 1 Curculionidae 7 1 Gammaridae 4 1 Rhyacophilidae 0 1 Elmidae 4 1 Chloroperlidae 1 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 107 % Contribution of Dominant Family: 40.19 % ( Ephemerellidae ) Family Biotic Index: 3.15 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 9 % EPT: 73.83 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 157 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 40 ' / 1-2 ' Substrate: Cobbles, gravel/sand, mud, silt....StreamBank Vegetation/Stability: Trees, shrubs/Fair Canopy: Partly Open....Other: Water temp. 12.2 C /pH 7.3 SU /DO 10.4 mg/L /Cond 165 umhos; Rural / forested trout stocked stream; salamanders, periphyton on rocks ------

Station: AN0325B Cakepoulin Creek, Rt 513, Franklin Twp, Hunterdon County Pittstown USGS Quadrangle Date Sampled: 04/29/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Ephemerellidae 1 40 Chironomidae 6 19 Naididae 7 7 Nemouridae 2 6 Baetidae 4 6 BloodRed Chironomidae 8 6 Heptageniidae 4 6 Tipulidae 3 3 Psephenidae 4 3 Hydropsychidae 4 2 Elmidae 4 2 Rhyacophilidae 0 2 Ceratopogonidae 6 1 Perlodidae 2 1 Lepidostomatidae 1 1 Lumbriculidae 8 1 Polycentropodidae 6 1 Simuliidae 6 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 108 % Contribution of Dominant Family: 37.04 % ( Ephemerellidae ) Family Biotic Index: 3.49 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 9 % EPT: 60.19 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 150 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 12 - 15 ' / 1 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Grasses, small trees, shrubs/Fair Canopy: Partly Open....Other: Water temp. 16.2 C /pH 7.4 SU /DO 10.8 mg/L /Cond 186 umhos; Urban / rural; stormwater pipe flowing on left bank boulder and concrete slabs on banks; salamanders, filamentous algae and macrophytes ------

Station: AN0326 S. Br. Raritan River, Stanton Rd., Readington Twp, Hunterdon County Flemington USGS Quadrangle Date Sampled: 04/29/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Lepidostomatidae 1 30 Elmidae 4 21 Glossosomatidae 0 10 Heptageniidae 4 9 Hydropsychidae 4 6 Gammaridae 4 4 Ephemerellidae 1 4 Perlidae 1 3 Corbiculidae 8 3 Chironomidae 6 2 Hydroptilidae 4 2 Psephenidae 4 2 Hydrobiidae 8 1 Tipulidae 3 1 Sphaeriidae 8 1 Simuliidae 6 1 ------Statistical Analysis ------Number of Taxa: 16 Total Number of Individuals: 100 % Contribution of Dominant Family: 30.00 % ( Lepidostomatidae ) Family Biotic Index: 2.74 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 7 % EPT: 64.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 143 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 100 ' / 2-3 ' Substrate: Cobbles, gravel/sand, silt....StreamBank Vegetation/Stability: Trees, shrubs/Fair Canopy: Mostly Open....Other: Water temp. 12.7 C /pH 7.5 SU /DO 10.4 mg/L /Cond 244 umhos; Rural / forested (Nature Preserve on righ bank) Trout stocked stream; USGS gage station; ------

Station: AN0327 Prescott Brook, Stanton Rd, Readington Twp, Hunterdon County Flemington USGS Quadrangle Date Sampled: 04/29/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Elmidae 4 18 Ephemerellidae 1 15 Psephenidae 4 15 Naididae 7 11 Chironomidae 6 10 Hydropsychidae 4 5 Philopotamidae 3 5 Nemouridae 2 4 Gammaridae 4 3 Perlidae 1 2 Glossosomatidae 0 2 Asellidae 8 2 Lepidostomatidae 1 2 Simuliidae 6 2 Heptageniidae 4 2 Leptoceridae 4 1 Gomphidae 1 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 100 % Contribution of Dominant Family: 18.00 % ( Elmidae ) Family Biotic Index: 3.84 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 9 % EPT: 38.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 167 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 20 ' / 1-2 ' Substrate: Cobbles....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Partly Open....Other: Water temp. 16.4 C /pH 7.6 SU /DO 9.0 mg/L /Cond 234 umhos; Rural / forested. Round Valley Resv upstream most of sample taken above trib on left bank; ------

Station: AN0328 Assiscong Creek, River Rd, Raritan Twp, Hunterdon County Flemington USGS Quadrangle Date Sampled: 05/04/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Nemouridae 2 34 Hydropsychidae 4 28 Baetidae 4 16 Perlodidae 2 6 Tipulidae 3 3 Ephemerellidae 1 3 Chironomidae 6 3 Chloroperlidae 1 1 Siphlonuridae 7 1 Calopterygidae 5 1 Lumbriculidae 8 1 Enchytraeidae 10 1 Naididae 7 1 BloodRed Chironomidae 8 1 Simuliidae 6 1 Elmidae 4 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 103 % Contribution of Dominant Family: 33.01 % ( Nemouridae ) Family Biotic Index: 3.36 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 8 % EPT: 87.38 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 178 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 20 ' / 1-2.5 ' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Trees, grass, shrubs/Poor Canopy: Open....Other: Water temp. 13.5 C /pH 7.7 SU /DO 11.4 mg/L /Cond 271 umhos; Agricultural-livestock / forested (S Br Reservation, Assiscong Marsh) salamanders; ------

Station: AN0329 S. Br. Raritan River, Rt 613 (Old York Rd), Readington Twp, Hunterdon County Flemington USGS Quadrangle Date Sampled: 05/04/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 55 Chironomidae 6 13 Naididae 7 5 Siphlonuridae 7 5 Elmidae 4 4 Corbiculidae 8 3 Sphaeriidae 8 3 Hydrobiidae 8 2 Nemouridae 2 2 Ephemerellidae 1 2 Lepidostomatidae 1 1 Tubificidae 10 1 Psephenidae 4 1 Heptageniidae 4 1 Chloroperlidae 1 1 Valvatidae 4 1 ------Statistical Analysis ------Number of Taxa: 16 Total Number of Individuals: 100 % Contribution of Dominant Family: 55.00 % ( Gammaridae ) Family Biotic Index: 4.78 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 6 % EPT: 12.00 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 145 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 130 ' / 2-3 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Grasses, trees, shrubs/Fair Canopy: Open....Other: Water temp. 14.7 C /pH 7.8 SU /DO 10.6 mg/L /Cond 235 umhos; Agriculture-livestock / suburban crayfish, yoy fish; ------

Station: AN0330 First Neshanic River, Rt 31, Raritan Twp, Hunterdon County Hopewell USGS Quadrangle Date Sampled: 05/11/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 51 Naididae 7 26 Lumbriculidae 8 24 Ephemerellidae 1 2 Gammaridae 4 2 Empididae 6 1 Perlodidae 2 1 Nematoda 6 1 Perlidae 1 1 ------Statistical Analysis ------Number of Taxa: 9 Total Number of Individuals: 109 % Contribution of Dominant Family: 46.79 % ( Chironomidae ) Family Biotic Index: 6.47 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 3.67 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 142 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 43 ' / 1 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, weeds/Fair Canopy: Mostly Open....Other: Water temp. 16.8 C /pH 7.6 SU /DO 8.3 mg/L /Cond 341 umhos; Commercial. 2 storm drains geese, fish; ------

Station: AN0331 Second Neshanic River, Rt 31, Raritan Twp, Hunterdon County Hopewell USGS Quadrangle Date Sampled: 05/11/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 41 Elmidae 4 20 Hydropsychidae 4 13 Psephenidae 4 9 Perlidae 1 8 Baetidae 4 4 Hydroptilidae 4 4 Planariidae 4 2 Naididae 7 2 Nemouridae 2 1 Asellidae 8 1 Ephemerellidae 1 1 Gammaridae 4 1 Heptageniidae 4 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 109 % Contribution of Dominant Family: 37.61 % ( Chironomidae ) Family Biotic Index: 4.57 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 7 % EPT: 29.36 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 137 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Slow....Width/Depth (ft): 43 ' / 1-2 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs, weeds/Fair Canopy: Mostly Open....Other: Water temp. 17.1 C /pH 7.6 SU /DO 7.1 mg/L /Cond 312 umhos; Commercial. Minnows and geese observed 3 absorbent oil booms in stream due to recent car fire and oil spill; slight oil sheen on water. ------

Station: AN0332 Third Neshanic River, Rt 31, Raritan Twp, Hunterdon County Hopewell USGS Quadrangle Date Sampled: 05/11/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 26 Elmidae 4 13 Hydropsychidae 4 10 Psephenidae 4 9 Ephemerellidae 1 8 Perlidae 1 7 Baetidae 4 6 Naididae 7 4 Heptageniidae 4 4 Hydrophilidae 5 2 Philopotamidae 3 2 Planariidae 4 2 Hydroptilidae 4 2 Tetrastemmatidae 7 2 Nemouridae 2 1 Helicopsychidae 3 1 Lepidostomatidae 1 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 100 % Contribution of Dominant Family: 26.00 % ( Chironomidae ) Family Biotic Index: 4.19 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 10 % EPT: 42.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 129 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 35 ' / 1 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs, weeds/Fair Canopy: Partly Open....Other: Water temp. 17.4 C /pH 7.6 SU /DO 8.3 mg/L /Cond 245 umhos; Commercial; storm sewer flowing periphyton, filamentous algae, minnows; ------

Station: AN0333 Neshanic River, Everitt Rd, East Amwell Twp, Hunterdon County Hopewell USGS Quadrangle Date Sampled: 05/11/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Naididae 7 32 Chironomidae 6 21 Elmidae 4 14 Psephenidae 4 7 Hydropsychidae 4 6 Perlidae 1 4 Baetidae 4 3 BloodRed Chironomidae 8 2 Planariidae 4 2 Heptageniidae 4 2 Hydrophilidae 5 1 Philopotamidae 3 1 Dytiscidae 5 1 Lumbriculidae 8 1 Nematoda 6 1 Cambaridae 5 1 Simuliidae 6 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 100 % Contribution of Dominant Family: 32.00 % ( Naididae ) Family Biotic Index: 5.44 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 16.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 130 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 71 ' / 1-2 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, grasses, vines/Fair Canopy: Mostly Open....Other: Water temp. 19.5 C /pH 8.0 SU /DO 10.5 mg/L /Cond 305 umhos; Agriculture-cropland / rural sampled downstream of weir; fish, frogs, water snake, minnows, crayfish, periphyton ------

Station: AN0334 , Wertsville Rd (Rt 602), East Amwell Twp, Hunterdon County Hopewell USGS Quadrangle Date Sampled: 05/11/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 30 Elmidae 4 13 Hydropsychidae 4 11 Naididae 7 10 Asellidae 8 7 Philopotamidae 3 7 Planariidae 4 6 Simuliidae 6 6 Gammaridae 4 3 Perlidae 1 2 Psephenidae 4 2 Tubificidae 10 1 Caenidae 7 1 BloodRed Chironomidae 8 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 100 % Contribution of Dominant Family: 30.00 % ( Chironomidae ) Family Biotic Index: 5.30 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 21.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 112 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 6 ' / 1-2 ' Substrate: Cobbles, bedrock....StreamBank Vegetation/Stability: Grasses, trees/Fair Canopy: Open....Other: Water temp. 22.3 C /pH 8.0 SU /DO 10.5 mg/L /Cond 397 umhos; Agriculture-livestock (horses) & Preserved farmland drainage ditch from farm flowing into stream; lg. fish, sunfish, periphyton, filamentous algae ------

Station: AN0335 Back Brook, Manners Rd (Rt 609), East Amwell Twp, Hunterdon County Hopewell USGS Quadrangle Date Sampled: 05/11/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 25 Caenidae 7 15 Perlidae 1 15 Lumbriculidae 8 10 Naididae 7 8 Baetidae 4 4 Psephenidae 4 4 Elmidae 4 4 BloodRed Chironomidae 8 3 Hydropsychidae 4 2 Tubificidae 10 2 Heptageniidae 4 2 Nemouridae 2 1 Hydrophilidae 5 1 Asellidae 8 1 Hydroptilidae 4 1 Perlodidae 2 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 100 % Contribution of Dominant Family: 25.00 % ( Chironomidae ) Family Biotic Index: 5.38 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 8 % EPT: 41.00 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 108 ( Marginal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 33 ' / 1-2 ' Substrate: Cobbles, gravel/sand, silt....StreamBank Vegetation/Stability: Trees, grasses, weeds/Fair Canopy: Mostly Open....Other: Water temp. 19.3 C /pH 7.8 SU /DO 10.9 mg/L /Cond 253 umhos; Rural / Agriculture-cropland (tree farm) storm sewers; minnows, tadpoles, periphyton ------

Station: AN0336 Furmans Brook, Welisewitz Rd, East Amwell Twp, Hunterdon County Hopewell USGS Quadrangle Date Sampled: 05/11/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Nemouridae 2 22 Baetidae 4 18 Chironomidae 6 12 Perlidae 1 11 Psephenidae 4 10 Heptageniidae 4 9 Leptophlebiidae 2 4 Ephemerellidae 1 3 Caenidae 7 2 Philopotamidae 3 2 Hydropsychidae 4 2 Perlodidae 2 2 Lumbriculidae 8 1 Naididae 7 1 Elmidae 4 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 22.00 % ( Nemouridae ) Family Biotic Index: 3.37 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 10 % EPT: 75.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 154 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 15 ' / < 1.0-1.0 ' Substrate: Cobbles, gravel/sand, red shale bedrock....StreamBank Vegetation/Stability: Trees, weeds/Fair Canopy: Mostly Closed....Other: Water temp. 20.7 C /pH 8.0 SU /DO 8.3 mg/L /Cond 160 umhos; Suburban / forested periphyton, minnows, crayfish; ------

Station: AN0337 Neshanic River, Rt 514 (Old York Rd), Hillsborough Twp, Somerset County Hopewell USGS Quadrangle Date Sampled: 05/06/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 52 Elmidae 4 10 Lumbriculidae 8 6 Heptageniidae 4 6 Caenidae 7 5 Naididae 7 5 Perlidae 1 5 BloodRed Chironomidae 8 4 Psephenidae 4 4 Leptophlebiidae 2 2 Baetidae 4 1 Leptoceridae 4 1 Hydropsychidae 4 1 Tubificidae 10 1 Nematoda 6 1 Sphaeriidae 8 1 Tetrastemmatidae 7 1 Tabanidae 6 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 108 % Contribution of Dominant Family: 48.15 % ( Chironomidae ) Family Biotic Index: 5.58 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 7 % EPT: 19.44 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 146 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 92 ' / 1-2 ' Substrate: Gravel....StreamBank Vegetation/Stability: Trees, shrubs, grass/Fair Canopy: Partly Open....Other: Water temp. 12.2 C /pH 7.6 SU /DO 9.8 mg/L /Cond 243 umhos; Rural / forested macrophytes; ------

Station: AN0338 S. Br. Raritan River, Elm St, Hillsborough Twp, Somerset County Raritan USGS Quadrangle Date Sampled: 05/06/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 30 Elmidae 4 12 Ephemerellidae 1 7 Gammaridae 4 6 Perlidae 1 5 Baetidae 4 5 Lepidostomatidae 1 4 Psephenidae 4 4 Pleuroceridae 6 3 Enchytraeidae 10 3 Heptageniidae 4 3 Caenidae 7 2 Hydropsychidae 4 2 Lumbricidae 10 2 Leptoceridae 4 2 BloodRed Chironomidae 8 2 Sphaeriidae 8 2 Potamanthidae 4 1 Philopotamidae 3 1 Helicopsychidae 3 1 Hydroptilidae 4 1 Pyralidae 5 1 Physidae 7 1 Simuliidae 6 1 ------Statistical Analysis ------Number of Taxa: 24 Total Number of Individuals: 101 % Contribution of Dominant Family: 29.70 % ( Chironomidae ) Family Biotic Index: 4.73 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 12 % EPT: 33.66 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 137 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 208 ' / 2-3 ' Substrate: Cobbles, gravel/sand, silt....StreamBank Vegetation/Stability: Trees, shrubs, grass/Fair Canopy: Mostly Open....Other: Water temp. 13.8 C /pH 7.6 SU /DO 10.6 mg/L /Cond 264 umhos; Rural. Downstream of historic dam ------

Station: AN0339 Pleasant Run, Pleasant Run Rd (Rt 629), Readington Twp, Hunterdon County Flemington USGS Quadrangle Date Sampled: 05/04/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 38 Nemouridae 2 18 Ephemerellidae 1 17 Gammaridae 4 4 Baetidae 4 3 Caenidae 7 3 Perlodidae 2 3 Naididae 7 3 Elmidae 4 3 Tubificidae 10 2 Planariidae 4 2 Heptageniidae 4 2 Tipulidae 3 1 Asellidae 8 1 Calopterygidae 5 1 Lumbriculidae 8 1 Perlidae 1 1 Sphaeriidae 8 1 Ceratopogonidae 6 1 Psephenidae 4 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 106 % Contribution of Dominant Family: 35.85 % ( Chironomidae ) Family Biotic Index: 4.23 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 7 % EPT: 44.34 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 156 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 22 ' / < 1.0 - 1.5 ' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Grass, few trees/shrubs/Good Canopy: Mostly Open....Other: Water temp. 12.6 C /pH 7.6 SU /DO 11.8 mg/L /Cond 173 umhos; Suburban. storm drain flowing from right bank minnows, snakes, salamander, periphyton; ------

Station: AN0340 Pleasant Run, South Branch Rd, Branchburg Twp, Somerset County Raritan USGS Quadrangle Date Sampled: 05/06/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Naididae 7 21 Chironomidae 6 20 Elmidae 4 16 Hydropsychidae 4 10 Ephemerellidae 1 7 Caenidae 7 5 Perlodidae 2 4 Psephenidae 4 4 Heptageniidae 4 4 Baetidae 4 2 Nemouridae 2 2 Tipulidae 3 1 Hydrophilidae 5 1 Philopotamidae 3 1 BloodRed Chironomidae 8 1 Gammaridae 4 1 Tubificidae 10 1 Lumbriculidae 8 1 Sphaeriidae 8 1 Polycentropodidae 6 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 104 % Contribution of Dominant Family: 20.19 % ( Naididae ) Family Biotic Index: 5.00 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 9 % EPT: 34.62 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 110 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 26 ' / 1-1.5 ' Substrate: Cobbles, gravel/sand, silt....StreamBank Vegetation/Stability: Grass/Poor Canopy: Open....Other: Water temp. 13.7 C /pH 7.7 SU /DO 11.5 mg/L /Cond 216 umhos; Agriculture-livestock geese, turtles, frogs; ------

Station: AN0341 S. Br. Raritan River, Studdiford Dr, Hillsborough Twp, Somerset County Raritan USGS Quadrangle Date Sampled: 05/06/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 35 Elmidae 4 21 Naididae 7 8 BloodRed Chironomidae 8 8 Sphaeriidae 8 7 Potamanthidae 4 6 Hydropsychidae 4 5 Heptageniidae 4 5 Gammaridae 4 4 Lumbriculidae 8 4 Perlidae 1 2 Glossiphoniidae 8 1 Hydrophilidae 5 1 Perlodidae 2 1 Valvatidae 4 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 109 % Contribution of Dominant Family: 32.11 % ( Chironomidae ) Family Biotic Index: 5.53 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 17.43 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 135 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Turbid....Flow: Moderate....Width/Depth (ft): 161 ' / 3 ' Substrate: Gravel/sand, silt....StreamBank Vegetation/Stability: Trees, grasses/Poor Canopy: Mostly Open....Other: Water temp. 14.4 C /pH 7.7 SU /DO 10.7 mg/L /Cond 254 umhos; Rural / Agriculture-livestock; storm sewers macrophytes, frogs, geese ; ------

Station: AN0342 Holland Brook, Holland Brook Rd, Readington Twp, Hunterdon County Flemington USGS Quadrangle Date Sampled: 05/04/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 35 Nemouridae 2 23 Hydropsychidae 4 10 Ephemerellidae 1 6 Leptoceridae 4 4 Lumbriculidae 8 3 Heptageniidae 4 3 Siphlonuridae 7 2 Baetidae 4 2 Aeshnidae 3 2 Elmidae 4 2 Limnephilidae 4 2 Tipulidae 3 1 Caenidae 7 1 Philopotamidae 3 1 Planorbidae 6 1 Perlodidae 2 1 Gomphidae 1 1 Lepidostomatidae 1 1 Naididae 7 1 Physidae 7 1 ------Statistical Analysis ------Number of Taxa: 21 Total Number of Individuals: 103 % Contribution of Dominant Family: 33.98 % ( Chironomidae ) Family Biotic Index: 4.22 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 12 % EPT: 54.37 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 152 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 8 ' / < 1.0 - 1.5 ' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Trees, grass, shrubs/Poor Canopy: Mostly Open....Other: Water temp. 14.2 C /pH 8.0 SU /DO 12.7 mg/L /Cond 182 umhos; Agriculture-cropland / rural / forested minnows, salamanders, periphyton; ------

Station: AN0343 Holland Brook, South Branch Rd, Branchburg Twp, Somerset County Raritan USGS Quadrangle Date Sampled: 05/06/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 42 Naididae 7 20 Gammaridae 4 6 Lumbriculidae 8 6 Elmidae 4 6 Tubificidae 10 5 Tetrastemmatidae 7 3 Heptageniidae 4 3 Lumbricidae 10 2 Psephenidae 4 2 Nemouridae 2 1 Caenidae 7 1 Planariidae 4 1 Empididae 6 1 Chloroperlidae 1 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 42.00 % ( Chironomidae ) Family Biotic Index: 6.19 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 6.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 111 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 56 ' / 2-2.5 ' Substrate: Gravel/sand....StreamBank Vegetation/Stability: Grass/Poor Canopy: Open....Other: Water temp. 14.7 C /pH 7.8 SU /DO 11.7 mg/L /Cond 252 umhos; Agriculture-livestock filamentous algae; ------

Station: AN0344 Unt To India Brook, Calais Rd, Randolph Twp, Morris County Mendham USGS Quadrangle Date Sampled: 05/19/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Naididae 7 52 Chironomidae 6 12 Philopotamidae 3 6 Elmidae 4 6 Sphaeriidae 8 6 Baetidae 4 4 Ephemerellidae 1 4 Simuliidae 6 3 Limnephilidae 4 2 Tubificidae 10 2 Perlidae 1 2 Nemouridae 2 1 Empididae 6 1 Tipulidae 3 1 Psephenidae 4 1 Leptophlebiidae 2 1 Leuctridae 0 1 BloodRed Chironomidae 8 1 Odontoceridae 0 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 107 % Contribution of Dominant Family: 48.60 % ( Naididae ) Family Biotic Index: 5.79 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 9 % EPT: 20.56 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 160 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 12 ' / < 1.0 ' Substrate: Cobbles, gravel/sand, silt....StreamBank Vegetation/Stability: Trees, shrubs, grass/Good Canopy: Mostly Closed....Other: Water temp. 18.0 C /pH 7.4 SU /DO 8.5 mg/L /Cond 265 umhos; Suburban; storm sewers pile of leaf litter dumped on right bank; ------

Station: AN0344A India Brook, Calais Rd, Randolph Twp, Morris County Mendham USGS Quadrangle Date Sampled: 05/19/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Ephemerellidae 1 19 Chironomidae 6 17 Lumbriculidae 8 16 Nemouridae 2 8 Naididae 7 7 Baetidae 4 6 Elmidae 4 6 Tipulidae 3 4 Perlodidae 2 3 Sphaeriidae 8 3 Gomphidae 1 3 Glossosomatidae 0 2 Leuctridae 0 2 Empididae 6 1 Perlidae 1 1 Planariidae 4 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 100 % Contribution of Dominant Family: 19.00 % ( Ephemerellidae ) Family Biotic Index: 4.25 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 7 % EPT: 41.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 115 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 14.5 ' / < 1.0 ' Substrate: Gravel/sand, silt....StreamBank Vegetation/Stability: Trees, shrubs/Fair Canopy: Mostly Closed....Other: Water temp. 15.4 C /pH 7.4 SU /DO 9.3 mg/L /Cond 381 umhos; Suburban stream channelized on both banks; sewage odor; fish ------

Station: AN0345 India Brook, Mountainside Rd, Mendham Twp, Morris County Mendham USGS Quadrangle Date Sampled: 05/19/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Philopotamidae 3 17 Ephemerellidae 1 15 Baetidae 4 13 Lumbriculidae 8 11 Glossosomatidae 0 9 Chironomidae 6 9 Elmidae 4 4 Perlidae 1 3 Psephenidae 4 3 Tipulidae 3 2 Hydropsychidae 4 2 Leuctridae 0 2 Pteronarcyidae 0 2 Nemouridae 2 1 Astacidae 5 1 Empididae 6 1 Gomphidae 1 1 Lepidostomatidae 1 1 Lumbricidae 10 1 BloodRed Chironomidae 8 1 Limnephilidae 4 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 22 Total Number of Individuals: 101 % Contribution of Dominant Family: 16.83 % ( Philopotamidae ) Family Biotic Index: 3.43 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 12 % EPT: 66.34 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 189 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 19 ' / 1 ' Substrate: Cobbles, boulders....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Closed....Other: Water temp. 16.4 C /pH 7.5 SU /DO 9.5 mg/L /Cond 237 umhos; Rural / forested (India Bk Natural Area - Green Acres land) ------

Station: AN0346 N. Br. Raritan River, Rt 24, Mendham Twp, Morris County Chester USGS Quadrangle Date Sampled: 05/19/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Naididae 7 39 Baetidae 4 32 Chironomidae 6 19 Philopotamidae 3 6 Lumbriculidae 8 4 Tipulidae 3 2 Asellidae 8 1 Ephemerellidae 1 1 Glossosomatidae 0 1 Hydroptilidae 4 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 107 % Contribution of Dominant Family: 36.45 % ( Naididae ) Family Biotic Index: 5.52 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 38.32 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 162 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 25 ' / < 1.0' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs/Fair Canopy: Mostly Closed....Other: Water temp. 16.7 C /pH 7.5 SU /DO 8.5 mg/L /Cond 297 umhos; Forested / suburban / agriculture-cropland storm sewers; nursery/tree farm adj to right bank; ------

Station: AN0347 Dawsons Brook, Ironia Rd (Off South Rd), Mendham Twp, Morris County Chester USGS Quadrangle Date Sampled: 05/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 18 Baetidae 4 12 Heptageniidae 4 12 Hydropsychidae 4 9 Ephemerellidae 1 7 Philopotamidae 3 6 Nemouridae 2 5 Tipulidae 3 5 Lepidostomatidae 1 4 Limnephilidae 4 4 Perlidae 1 3 Elmidae 4 3 Psephenidae 4 3 Gomphidae 1 2 Rhyacophilidae 0 2 Glossosomatidae 0 1 Ptilodactylidae 1 1 Astacidae 5 1 Lumbricidae 10 1 Lumbriculidae 8 1 Psychomyiidae 2 1 Pteronarcyidae 0 1 ------Statistical Analysis ------Number of Taxa: 22 Total Number of Individuals: 102 % Contribution of Dominant Family: 17.65 % ( Chironomidae ) Family Biotic Index: 3.58 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 13 % EPT: 65.69 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 145 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 10 ' / < 1.0' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs, weeds/Good Canopy: Partly Open....Other: Water temp. 15.4 C /pH 8.0 SU /DO 9.0 mg/L /Cond 241 umhos; Suburban / forested homes and yards adjacent to stream; ------

Station: AN0348 Burnett Brook, Old Mill Rd, Mendham Twp, Morris County Chester USGS Quadrangle Date Sampled: 05/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 18 Lumbriculidae 8 15 Philopotamidae 3 14 Ephemerellidae 1 14 Baetidae 4 6 Nemouridae 2 5 Tipulidae 3 5 Psephenidae 4 4 Elmidae 4 3 Perlodidae 2 2 Leuctridae 0 2 Naididae 7 2 Simuliidae 6 2 Glossosomatidae 0 1 Empididae 6 1 Hydropsychidae 4 1 Gomphidae 1 1 Limnephilidae 4 1 Peltoperlidae 1 1 Pteronarcyidae 0 1 Rhyacophilidae 0 1 ------Statistical Analysis ------Number of Taxa: 21 Total Number of Individuals: 100 % Contribution of Dominant Family: 18.00 % ( Chironomidae ) Family Biotic Index: 4.07 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 12 % EPT: 49.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 163 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 12 ' / < 1.0 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, weeds/Good Canopy: Mostly Closed....Other: Water temp. 14.4 C /pH 8.1 SU /DO 10.4 mg/L /Cond 276 umhos; Suburban / forested salamanders, water snakes (mating - 8); ------

Station: AN0349 Peapack Brook, Fox Chase Rd Off Rt 512, Chester Twp, Morris County Chester USGS Quadrangle Date Sampled: 05/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Ephemerellidae 1 19 Limnephilidae 4 15 Lumbriculidae 8 10 Naididae 7 8 Chironomidae 6 8 Baetidae 4 7 Philopotamidae 3 5 Gammaridae 4 5 Tipulidae 3 3 Brachycentridae 1 3 Elmidae 4 3 Glossosomatidae 0 2 Leuctridae 0 2 Planariidae 4 2 Rhyacophilidae 0 2 Heptageniidae 4 2 BloodRed Chironomidae 8 1 Hydroptilidae 4 1 Lepidostomatidae 1 1 Polycentropodidae 6 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 100 % Contribution of Dominant Family: 19.00 % ( Ephemerellidae ) Family Biotic Index: 3.85 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 12 % EPT: 60.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 167 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 24 ' / < 1.0 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs, weeds/Good Canopy: Mostly Closed....Other: Water temp. 14.8 C /pH 8.0 SU /DO 8.7 mg/L /Cond 288 umhos; Suburban small pond feeding stream; lg fish, frogs ------

Station: AN0350 Peapack Brook, Old Dutch Rd, Off Rt 512, Bedminster Twp, Somerset County Gladstone USGS Quadrangle Date Sampled: 05/13/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 36 Elmidae 4 17 Baetidae 4 13 Ephemerellidae 1 12 Hydropsychidae 4 8 Naididae 7 5 Psephenidae 4 4 Philopotamidae 3 2 Simuliidae 6 2 Heptageniidae 4 2 Perlidae 1 1 Nemouridae 2 1 Tipulidae 3 1 Asellidae 8 1 Gammaridae 4 1 Nematoda 6 1 BloodRed Chironomidae 8 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 108 % Contribution of Dominant Family: 33.33 % ( Chironomidae ) Family Biotic Index: 4.53 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 7 % EPT: 36.11 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 142 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 20 ' / 1.5 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs/Fair Canopy: Partly Open....Other: Water temp. 18.6 C /pH 7.8 SU /DO 9.6 mg/L /Cond 344 umhos; Suburban / rural / forested ------

Station: AN0351 N. Br Raritan River, Rt 202, Far Hills Boro, Somerset County Gladstone USGS Quadrangle Date Sampled: 05/13/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 30 Elmidae 4 19 Ephemerellidae 1 14 Baetidae 4 12 Lumbriculidae 8 8 Naididae 7 5 Perlidae 1 2 Philopotamidae 3 2 Limnephilidae 4 2 Psephenidae 4 2 Heptageniidae 4 2 Planariidae 4 1 Lumbricidae 10 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 100 % Contribution of Dominant Family: 30.00 % ( Chironomidae ) Family Biotic Index: 4.63 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 6 % EPT: 34.00 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 147 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 64 ' / 3 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs/Fair Canopy: Mostly Open....Other: Water temp. 20.1 C /pH 7.8 SU /DO 9.2 mg/L /Cond 265 umhos; Suburban; storm sewers ------

Station: AN0352 Mine Brook, Old Quarry Rd, Bernardsville Boro, Somerset County Bernardsville USGS Quadrangle Date Sampled: 05/13/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Simuliidae 6 34 Lumbriculidae 8 25 Naididae 7 19 Chironomidae 6 9 Planariidae 4 4 Gammaridae 4 2 Sphaeriidae 8 2 Baetidae 4 1 BloodRed Chironomidae 8 1 Philopotamidae 3 1 Enchytraeidae 10 1 Hydroptilidae 4 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 100 % Contribution of Dominant Family: 34.00 % ( Simuliidae ) Family Biotic Index: 6.60 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 3.00 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 147 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 9 ' / 1 ' Substrate: Cobbles, gravel/sand, concrete....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Mostly Closed....Other: Water temp. 18.3 C/pH 7.6 SU /DO 8.6 mg/L /Cond 426 umhos; Industrial / suburban; baseball field adj to right bank storm sewers and possible sports field drainage pipe; ------

Station: AN0353 Mine Brook, Far Hills Rd, Far Hills Boro, Somerset County Gladstone USGS Quadrangle Date Sampled: 06/24/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Elmidae 4 32 Gammaridae 4 14 Chironomidae 6 14 BloodRed Chironomidae 8 7 Baetidae 4 6 Hydropsychidae 4 6 Tubificidae 10 5 Philopotamidae 3 3 Planariidae 4 3 Psephenidae 4 3 Asellidae 8 2 Naididae 7 2 Athericidae 2 1 Lepidostomatidae 1 1 Leptoceridae 4 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 32.00 % ( Elmidae ) Family Biotic Index: 4.92 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 17.00 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 115 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 27 ' / 1.5 ' Substrate: Gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs, grass/Fair Canopy: Mostly Closed....Other: Water temp. 20.6 C /pH 7.4 SU /DO 8.5 mg/L /Cond 378 umhos; Suburban; storm sewers pipe for pump in water & sand bags in stream; rock wall on left bank; fish ------

Station: AN0354 Middle Brook, Spook Hollow Rd, Bedminster Twp, Somerset County Gladstone USGS Quadrangle Date Sampled: 05/13/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 70 Naididae 7 19 BloodRed Chironomidae 8 3 Baetidae 4 2 Elmidae 4 2 Caenidae 7 1 Lumbriculidae 8 1 Tetrastemmatidae 7 1 Simuliidae 6 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 10 Total Number of Individuals: 101 % Contribution of Dominant Family: 69.31 % ( Chironomidae ) Family Biotic Index: 6.19 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 3.96 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 136 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Chironomidae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 9.5 ' / 1 ' Substrate: Cobbles, gravel/sand, silt....StreamBank Vegetation/Stability: Trees, shrubs/Fair Canopy: Mostly Closed....Other: Water temp. 18.6 C /pH 7.7 SU /DO 9.4 mg/L /Cond 210 umhos; Forested / agriculture-cropland ------

Station: AN0355 Middle Brook, Cutting Witney Road (River Road); Bedminster Twp., Somerset County Glandstone USGS Quadrangle Date Sampled: 04/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 25 Elmidae 4 22 Hydropsychidae 4 8 Psephenidae 4 5 Tipulidae 3 4 Empididae 6 4 Lepidostomatidae 1 4 Lumbriculidae 8 4 Tetrastemmatidae 7 4 Simuliidae 6 4 Philopotamidae 3 3 Tubificidae 10 3 Ephemerellidae 1 2 Hydroptilidae 4 2 BloodRed Chironomidae 8 2 Planariidae 4 1 Gomphidae 1 1 Lumbricidae 10 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 100 % Contribution of Dominant Family: 25.00 % ( Chironomidae ) Family Biotic Index: 4.98 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 6 % EPT: 20.00 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 130 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 16' / 1' Substrate: Cobbles, gravel, sand....StreamBank Vegetation/Stability: Trees, shrubs/Fair Canopy: Partly Open....Other: Rural, Forested; Water temp. 16.8 C / pH 7.7 SU / DO 11.2 mg/L / Cond. 210 umhos Filamentous Algae; Fish; ------

Station: AN0356 Lamington River, Ironia Rd, Chester Twp, Morris County Chester USGS Quadrangle Date Sampled: 05/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 86 Asellidae 8 9 Chironomidae 6 2 Naididae 7 1 Leptoceridae 4 1 Tubificidae 10 1 ------Statistical Analysis ------Number of Taxa: 6 Total Number of Individuals: 100 % Contribution of Dominant Family: 86.00 % ( Gammaridae ) Family Biotic Index: 4.49 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 1.00 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 119 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Gammaridae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid, cedar colored....Flow: Slow....Width/Depth (ft): 33 ' / 2 ' Substrate: Gravel/sand, silt....StreamBank Vegetation/Stability: Trees, shrubs, weeds/Fair Canopy: Open....Other: Water temp. 18.7 C /pH 7.8 SU /DO 7.9 mg/L /Cond 473 umhos; Suburban / forested. USGS gage station geese; ------

Station: AN0357 Tanners Brook, Tanners Brook Rd, Chester Twp, Morris County Chester USGS Quadrangle Date Sampled: 05/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 34 Sphaeriidae 8 16 Chironomidae 6 10 Limnephilidae 4 10 Lumbriculidae 8 7 Baetidae 4 5 Elmidae 4 4 Heptageniidae 4 4 Simuliidae 6 3 Ephemerellidae 1 2 Tubificidae 10 2 Nemouridae 2 1 Hydropsychidae 4 1 BloodRed Chironomidae 8 1 Glossosomatidae 0 1 Lumbricidae 10 1 Naididae 7 1 Tabanidae 6 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 104 % Contribution of Dominant Family: 32.69 % ( Gammaridae ) Family Biotic Index: 5.28 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 7 % EPT: 23.08 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 106 ( Marginal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 8 ' / < 1.0' Substrate: Sand, silt....StreamBank Vegetation/Stability: Shrubs, vines, weeds/Poor Canopy: Mostly Open....Other: Water temp. 16.3 C /pH 8.0 SU /DO 8.2 mg/L /Cond 175 umhos; Forested / suburban (new housing development under construction-left bank) both banks eroded; frogs, minnows, macrophytes ------

Station: AN0358 Lamington River, Rt 24 (Cooper Mill Park), Chester Twp, Morris County Chester USGS Quadrangle Date Sampled: 05/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 33 Gammaridae 4 22 Hydropsychidae 4 14 Simuliidae 6 5 Elmidae 4 5 Baetidae 4 4 Asellidae 8 4 Brachycentridae 1 3 Naididae 7 2 Sphaeriidae 8 2 Hydrobiidae 8 1 Nemouridae 2 1 Cambaridae 5 1 Planariidae 4 1 Leptoceridae 4 1 Physidae 7 1 ------Statistical Analysis ------Number of Taxa: 16 Total Number of Individuals: 100 % Contribution of Dominant Family: 33.00 % ( Chironomidae ) Family Biotic Index: 5.03 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 23.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 191 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 24 ' / 2 ' Substrate: Cobbles, boulders....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Mostly Closed....Other: Water temp. 19.3 C /pH 7.7 SU /DO 7.4 mg/L /Cond 322 umhos; Suburban / forested periphyton, macrophytes, many emerged stonefly exoskeletons; ------

Station: AN0359 Trout Brook, State Park Rd (Near Hacklebarney Sp), Chester Twp, Morris County Chester USGS Quadrangle Date Sampled: 05/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 25 Lumbriculidae 8 13 Philopotamidae 3 12 Ephemerellidae 1 11 Baetidae 4 10 Naididae 7 6 Hydroptilidae 4 4 Gammaridae 4 3 Tipulidae 3 2 Leuctridae 0 2 Elmidae 4 2 Nemouridae 2 1 Perlidae 1 1 Psephenidae 4 1 Gomphidae 1 1 Hydropsychidae 4 1 Perlodidae 2 1 Lepidostomatidae 1 1 Lumbricidae 10 1 Polycentropodidae 6 1 Tabanidae 6 1 ------Statistical Analysis ------Number of Taxa: 21 Total Number of Individuals: 100 % Contribution of Dominant Family: 25.00 % ( Chironomidae ) Family Biotic Index: 4.62 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 11 % EPT: 45.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 166 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 11 ' / < 1.0' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Trees, shrubs, weeds/Good Canopy: Closed....Other: Water temp.15.4 C /pH 7.8 SU / DO 9.7 mg/L /Cond 211 umhos; Forested / suburban ------

Station: AN0360 Lamington River, Rt 512, Tewksbury Twp, Hunterdon/Somerset County Gladstone USGS Quadrangle Date Sampled: 05/25/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 22 Elmidae 4 21 Brachycentridae 1 12 Ephemerellidae 1 9 Baetidae 4 7 Perlidae 1 6 Hydropsychidae 4 4 Lepidostomatidae 1 4 Limnephilidae 4 3 Psephenidae 4 3 Chloroperlidae 1 1 Blephariceridae 0 1 Gammaridae 4 1 Glossosomatidae 0 1 Perlodidae 2 1 Lumbricidae 10 1 Naididae 7 1 Pteronarcyidae 0 1 Simuliidae 6 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 100 % Contribution of Dominant Family: 22.00 % ( Chironomidae ) Family Biotic Index: 3.45 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 11 % EPT: 49.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 157 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 38 ' / < 1.0 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, weeds, shrubs/Good Canopy: Mostly Closed....Other: Water temp. 20.0 C /pH 7.9 SU /DO 8.9 mg/L /Cond 298 umhos; Suburban frogs, fish; ------

Station: AN0361 Unt To Lamington River, Black River Rd, Bedminster Twp, Somerset County Gladstone USGS Quadrangle Date Sampled: 05/25/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Baetidae 4 38 Chironomidae 6 25 Simuliidae 6 6 Lumbriculidae 8 5 Ephemerellidae 1 4 Hydropsychidae 4 4 Philopotamidae 3 3 Elmidae 4 3 Perlidae 1 3 Nemouridae 2 2 Heptageniidae 4 2 Glossosomatidae 0 1 Tipulidae 3 1 Empididae 6 1 Leuctridae 0 1 Naididae 7 1 ------Statistical Analysis ------Number of Taxa: 16 Total Number of Individuals: 100 % Contribution of Dominant Family: 38.00 % ( Baetidae ) Family Biotic Index: 4.50 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 9 % EPT: 58.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 161 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 24 ' / < 1.0 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, weeds, shrubs/Fair Canopy: Mostly Open....Other: Water temp. 18.2 C /pH 7.9 SU /DO 8.1 mg/L /Cond 205 umhos; Rural / forested fish, yoy minnows; ------

Station: AN0362 Cold Brook, Vliettown Road, Tewksbury Twp, Hunterdon County Gladstone USGS Quadrangle Date Sampled: 05/25/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Elmidae 4 28 Chironomidae 6 18 Baetidae 4 13 Hydropsychidae 4 10 Simuliidae 6 8 Ephemerellidae 1 6 Gammaridae 4 5 Limnephilidae 4 4 Psephenidae 4 3 Philopotamidae 3 2 Gomphidae 1 2 Lumbriculidae 8 2 Rhyacophilidae 0 2 Perlodidae 2 1 Naididae 7 1 Tetrastemmatidae 7 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 107 % Contribution of Dominant Family: 26.17 % ( Elmidae ) Family Biotic Index: 4.27 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 7 % EPT: 35.51 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 137 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 31 ' / 1 - 3 ' Substrate: Silt, gravel....StreamBank Vegetation/Stability: Shrubs, trees, grasses/Fair Canopy: Mostly Closed....Other: Water temp. 16.7 C /pH 7.6 SU /DO 8.3 mg/L /Cond 293 umhos; Rural / Agriculture-livestock (horses) macrophytes, green frogs; ------

Station: AN0363 Lamington River, Rt 523, Bedminster Twp, Somerset/Hunterdon County Gladstone USGS Quadrangle Date Sampled: 05/25/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Elmidae 4 24 Baetidae 4 23 Chironomidae 6 16 Ephemerellidae 1 7 Heptageniidae 4 7 Hydropsychidae 4 4 Gammaridae 4 4 Perlidae 1 3 Glossosomatidae 0 2 Helicopsychidae 3 2 Lumbricidae 10 2 Limnephilidae 4 2 Psephenidae 4 2 Leuctridae 0 1 Ceratopogonidae 6 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 24.00 % ( Elmidae ) Family Biotic Index: 4.02 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 9 % EPT: 51.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 156 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear, cedar....Flow: Moderate....Width/Depth (ft): 64 ' / < 1.0 ' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Trees, grasses/Good Canopy: Partly Open....Other: Water temp. 19.8 C /pH 7.5 SU /DO 7.3 mg/L /Cond 289 umhos; Rural / forested macrophytes, large fish; ------

Station: AN0364 N. Br. Rockaway Creek, Fairmount Rd (Rt 512), Tewksbury Twp, Hunterdon County Califon USGS Quadrangle Date Sampled: 04/27/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Ephemerellidae 1 43 Nemouridae 2 13 Baetidae 4 6 Lumbriculidae 8 6 Chironomidae 6 6 Tipulidae 3 4 Glossosomatidae 0 2 Ptilodactylidae 1 2 Hydropsychidae 4 2 Perlodidae 2 2 Leuctridae 0 2 Brachycentridae 1 2 Naididae 7 2 Simuliidae 6 2 Dryopidae 5 1 Lepidostomatidae 1 1 Limnephilidae 4 1 Elmidae 4 1 Chloroperlidae 1 1 Peltoperlidae 1 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 100 % Contribution of Dominant Family: 43.00 % ( Ephemerellidae ) Family Biotic Index: 2.47 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 12 % EPT: 76.00 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 154 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 11 ' / 1 - 2 ' Substrate: Sand, silt....StreamBank Vegetation/Stability: Shrubs, trees/Fair Canopy: Mostly Open....Other: Water temp. 13.8 C /pH 7.3 SU /DO 6.2 mg/L /Cond 132 umhos; Rural / Agriculture-livestock periphyton, fish and salamanders observed; ------

Station: AN0365 N. Br. Rockaway Creek, Rockaway Rd, Tewksbury Twp, Hunterdon County Califon USGS Quadrangle Date Sampled: 04/27/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Ephemerellidae 1 36 Baetidae 4 23 Nemouridae 2 6 Psephenidae 4 5 Chironomidae 6 4 Simuliidae 6 4 Tubificidae 10 3 Peltoperlidae 1 3 BloodRed Chironomidae 8 3 Lumbriculidae 8 2 Naididae 7 2 Glossosomatidae 0 1 Hydropsychidae 4 1 Tipulidae 3 1 Chloroperlidae 1 1 Empididae 6 1 Brachycentridae 1 1 Limnephilidae 4 1 Pteronarcyidae 0 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 100 % Contribution of Dominant Family: 36.00 % ( Ephemerellidae ) Family Biotic Index: 3.18 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 11 % EPT: 75.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 144 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 47.5 ' / 1-2 ' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Grasses, trees, shrubs/Fair Canopy: Mostly Open....Other: Water temp. 12.0 C /pH 7.3 SU /DO 11.5 mg/L /Cond 200 umhos; Rural organic yard debris dumped on bank; ------

Station: AN0366 N. Br Rockaway Creek, Rockaway Rd / Taylor's Mill Rd, Readington Twp, Hunterdon County Califon USGS Quadrangle Date Sampled: 04/27/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Ephemerellidae 1 34 Baetidae 4 12 Chironomidae 6 11 Nemouridae 2 8 Simuliidae 6 5 Hydropsychidae 4 4 Gammaridae 4 3 Lepidostomatidae 1 3 Lumbriculidae 8 3 Philopotamidae 3 2 Tubificidae 10 2 Planorbidae 6 2 Siphlonuridae 7 2 Glossosomatidae 0 1 Empididae 6 1 Heptageniidae 4 1 Perlidae 1 1 Sphaeriidae 8 1 Polycentropodidae 6 1 Psephenidae 4 1 Limnephilidae 4 1 Leptoceridae 4 1 ------Statistical Analysis ------Number of Taxa: 22 Total Number of Individuals: 100 % Contribution of Dominant Family: 34.00 % ( Ephemerellidae ) Family Biotic Index: 3.38 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 13 % EPT: 71.00 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 180 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 40 ' / 1.5 - 2 ' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Trees, grasses, shrubs/Good Canopy: Partly Open....Other: Water temp. 13.0 C /pH 7.3 SU /DO 10.9 mg/L /Cond 130 umhos; Forested / industrial (downstream of quarry) salamander, fish, & crayfish. ; fresh creosote on bridge ------

Station: AN0367 S. Br. Rockaway Creek, Windy Acres Farm, Lebanon Boro, Hunterdon County Califon USGS Quadrangle Date Sampled: 05/04/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 42 Naididae 7 13 Ephemerellidae 1 7 Elmidae 4 6 Asellidae 8 5 Gammaridae 4 5 Hydropsychidae 4 5 Lumbriculidae 8 3 Tipulidae 3 2 Tubificidae 10 2 Heptageniidae 4 2 Perlidae 1 1 Calopterygidae 5 1 Philopotamidae 3 1 Empididae 6 1 BloodRed Chironomidae 8 1 Psephenidae 4 1 Simuliidae 6 1 Siphlonuridae 7 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 100 % Contribution of Dominant Family: 42.00 % ( Chironomidae ) Family Biotic Index: 5.52 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 6 % EPT: 17.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 165 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 12.5 ' / 1-1.5 ' Substrate: Cobbles, bedrock....StreamBank Vegetation/Stability: Grasses, trees, shrubs/Poor Canopy: Mostly Open....Other: Water temp. 10.9 C /pH 7.6 SU /DO 11.7 mg/L /Cond 333 umhos; Suburban / Agriculture-cropland fish; ------

Station: AN0368 S. Br. Rockaway Creek, Rt 22, Readington Twp, Hunterdon County Flemington USGS Quadrangle Date Sampled: 05/04/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 31 Gammaridae 4 17 Asellidae 8 15 Naididae 7 9 Simuliidae 6 7 Sphaeriidae 8 6 Elmidae 4 6 Planorbidae 6 2 BloodRed Chironomidae 8 2 Psephenidae 4 2 Planariidae 4 1 Ephemerellidae 1 1 Ephydridae 6 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 100 % Contribution of Dominant Family: 31.00 % ( Chironomidae ) Family Biotic Index: 5.98 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 1.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 171 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Moderate....Width/Depth (ft): 22 ' / < 1.0 - 1.5 ' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Trees, grass, shrubs/Good Canopy: Mostly Open....Other: Water temp. 13.3 C /pH 7.5 SU /DO 10.3 mg/L /Cond 310 umhos; Suburban. Storm drain flowing minnows, trash; extremely turbid water; ------

Station: AN0369 Rockaway Creek, Island Road; Readington Twp., Hunterdon County Raritan USGS Quadrangle Date Sampled: 04/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 45 Elmidae 4 19 BloodRed Chironomidae 8 9 Tubificidae 10 8 Psephenidae 4 4 Potamanthidae 4 2 Gammaridae 4 2 Hydropsychidae 4 2 Lepidostomatidae 1 2 Perlidae 1 1 Tipulidae 3 1 Tabanidae 6 1 Planariidae 4 1 Lumbricidae 10 1 Lumbriculidae 8 1 Naididae 7 1 Sphaeriidae 8 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 102 % Contribution of Dominant Family: 44.12 % ( Chironomidae ) Family Biotic Index: 5.79 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 7.84 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 104 ( Marginal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 80' / 2' Substrate: Cobbles, gravel, sand, silt....StreamBank Vegetation/Stability: Grasses, trees/Poor Canopy: Open....Other: Agriculture-livestock; Water temp. 16.3 C / pH 7.6 SU / DO 11.8 mg/L / Cond. 218 umhos Fish; ------

Station: AN0370 Lamington River, Cowperthwaite Road, Branchburg Twp., Somerset County Gladstone USGS Quadrangle Date Sampled: 4/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Elmidae 4 37 Hydropsychidae 4 15 Chironomidae 6 10 Psephenidae 4 5 Empididae 6 4 Ephemerellidae 1 4 Glossosomatidae 0 4 Limnephilidae 4 4 Perlidae 1 3 Gammaridae 4 3 Naididae 7 3 Potamanthidae 4 2 Tipulidae 3 2 Philopotamidae 3 1 Lepidostomatidae 1 1 Lumbricidae 10 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 100 % Contribution of Dominant Family: 37.00 % ( Elmidae ) Family Biotic Index: 4.00 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 9 % EPT: 35.00 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 137 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 105' / 2' Substrate: Gravel, sand....StreamBank Vegetation/Stability: Trees, grass/Poor Canopy: Mostly open....Other: Suburban; Water temp. 10.6 C / pH 7.7 SU / DO 16.5 mg/L / Cond. 245 umhos Fish; ------

Station: AN0371 Chambers (B) Brook, Love Rd, Bedminster Twp, Somerset County Raritan USGS Quadrangle Date Sampled: 04/20/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Tubificidae 10 39 Elmidae 4 21 Chironomidae 6 15 Psephenidae 4 9 Tetrastemmatidae 7 3 Coenagrionidae 9 2 Hydropsychidae 4 2 BloodRed Chironomidae 8 2 Gomphidae 1 2 Philopotamidae 3 1 Planariidae 4 1 Gammaridae 4 1 Lumbriculidae 8 1 Sphaeriidae 8 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 100 % Contribution of Dominant Family: 39.00 % ( Tubificidae ) Family Biotic Index: 6.92 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 3.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 128 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 21 ' / < 1.0 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, weeds/Fair Canopy: Partly Open....Other: Water temp. 17.0 C /pH 8.1 SU /DO 13.8 mg/L /Cond 597 umhos; Rural / forested fish; ------

Station: AN0372 Chambers (A) Brook, Coddington Rd, Readington Twp, Hunterdon County Raritan USGS Quadrangle Date Sampled: 06/02/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Psephenidae 4 20 Elmidae 4 20 Hydropsychidae 4 17 Chironomidae 6 13 Perlidae 1 11 BloodRed Chironomidae 8 9 Glossosomatidae 0 4 Heptageniidae 4 3 Dytiscidae 5 2 Gomphidae 1 2 Hydroptilidae 4 1 Philopotamidae 3 1 Corixidae 9 1 Gyrinidae 3 1 Gerridae 8 1 Tetrastemmatidae 7 1 Sialidae 4 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 108 % Contribution of Dominant Family: 18.52 % ( Psephenidae & Elmidae ) Family Biotic Index: 4.18 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 6 % EPT: 34.26 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 139 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 19 ' / < 1.0 ' Substrate: Cobbles, gravel/sand, silt....StreamBank Vegetation/Stability: Trees, shrubs, grass/Fair Canopy: Mostly Closed....Other: Water temp. 15.2 C /pH 7.6 SU /DO 9.7 mg/L /Cond 272 umhos; Rural salamanders; ------

Station: AN0373 Chambers (A) Brook, Station Rd, Branchburg Twp, Somerset County Raritan USGS Quadrangle Date Sampled: 06/02/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 45 Hydropsychidae 4 14 Elmidae 4 9 Naididae 7 8 Philopotamidae 3 7 Gammaridae 4 4 Tipulidae 3 2 Erpobdellidae 8 2 Simuliidae 6 2 Sphaeriidae 8 2 Coenagrionidae 9 1 Planariidae 4 1 Nematoda 6 1 Perlidae 1 1 Psephenidae 4 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 45.00 % ( Chironomidae ) Family Biotic Index: 5.29 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 22.00 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 163 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 20 ' / < 1.0 ' Substrate: Cobbles....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Mostly Closed....Other: Water temp. 16.3 C /pH 7.5 SU /DO 8.7 mg/L /Cond 340 umhos; Rural storm sewers; ------

Station: AN0374 N Br Raritan River, Rt 202, Branchburg Twp, Somerset County Raritan USGS Quadrangle Date Sampled: 06/02/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 20 Elmidae 4 17 Potamanthidae 4 14 Perlidae 1 13 Hydropsychidae 4 12 Psephenidae 4 6 Heptageniidae 4 4 Chironomidae 6 4 Baetidae 4 3 Tetrastemmatidae 7 2 Caenidae 7 1 Planariidae 4 1 Ephemerellidae 1 1 Tubificidae 10 1 Nematoda 6 1 BloodRed Chironomidae 8 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 102 % Contribution of Dominant Family: 19.61 % ( Gammaridae ) Family Biotic Index: 3.86 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 7 % EPT: 47.06 NJIS Rating: 30 Biological Condition: Nonimpaired Habitat Analysis: 156 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Turbid....Flow: Moderate....Width/Depth (ft): 120 ' / 3 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Open....Other: Water temp. 16.6 C /pH 7.7 SU /DO 9.0 mg/L /Cond 204 umhos; Suburban storm sewers; ------

Station: AN0375 Dukes Brook, Dukes Parkway, Hillsborough Twp, Somerset County Bound Brook USGS Quadrangle Date Sampled: 06/02/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 61 Asellidae 8 17 BloodRed Chironomidae 8 11 Chironomidae 6 5 Sphaeriidae 8 4 Hydrobiidae 8 3 Coenagrionidae 9 2 Physidae 7 2 Corixidae 9 1 Cambaridae 5 1 Elmidae 4 1 Valvatidae 4 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 109 % Contribution of Dominant Family: 55.96 % ( Gammaridae ) Family Biotic Index: 5.58 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 130 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 22 ' / < 1.0 ' Substrate: Cobbles, gravel/sand, silt....StreamBank Vegetation/Stability: Trees, shrubs, grass/Fair Canopy: Mostly Closed....Other: Water temp. 19.5 C /pH 7.6 SU /DO 7.2 mg/L /Cond 293 umhos; Suburban fish; ------

Station: AN0376 Peters Brook, Route 28, Somerville Boro, Somerset County Bound Brook USGS Quadrangle Date Sampled: 06/02/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 25 BloodRed Chironomidae 8 24 Tubificidae 10 14 Chironomidae 6 13 Elmidae 4 8 Coenagrionidae 9 5 Naididae 7 4 Tipulidae 3 1 Asellidae 8 1 Calopterygidae 5 1 Cambaridae 5 1 Libellulidae 9 1 Lumbriculidae 8 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 100 % Contribution of Dominant Family: 25.00 % ( Gammaridae ) Family Biotic Index: 6.60 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 113 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Slow....Width/Depth (ft): 46' / 1' Substrate: Gravel/sand, Silt....StreamBank Vegetation/Stability: Trees, Shrubs/Fair Canopy: Partly Open....Other: Water temp. 18.3 C / pH 7.5 SU / DO 7.9 mg/L / Cond. 540 umhos; Urban Storm sewers present; park on right bank ------

Station: AN0377 Raritan River, Abv Millstone River Confluence, Manville Boro, Somerset County Bound Brook USGS Quadrangle Date Sampled: 06/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 86 Elmidae 4 3 Tubificidae 10 2 Hydrobiidae 8 1 Asellidae 8 1 Corixidae 9 1 Hydropsychidae 4 1 Cambaridae 5 1 Haliplidae 5 1 Perlidae 1 1 Sphaeriidae 8 1 Psephenidae 4 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 100 % Contribution of Dominant Family: 86.00 % ( Gammaridae ) Family Biotic Index: 4.28 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 2.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 118 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Gammaridae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Moderate....Width/Depth (ft): > 100 ' / 1 - 2 ' Substrate: Cobbles, gravel, mud....StreamBank Vegetation/Stability: Grasses, trees, shrubs/Poor Canopy: Open....Other: Water temp. 21.2 C /pH 7.4 SU /DO 7.7 mg/L /Cond 266 umhos; Suburban / forested / light industrial periphyton, filamentous algae, macrophytes; ------

Station: AN0378 Millstone River, Baird Rd., Millstone Twp., Monmouth County Roosevelt USGS Quadrangle Date Sampled: 08/05/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Heptageniidae 4 39 Chironomidae 6 17 Sphaeriidae 8 11 Elmidae 4 7 Tubificidae 10 6 Hydropsychidae 4 5 Tipulidae 3 3 BloodRed Chironomidae 8 2 Gomphidae 1 2 Sialidae 4 2 Calopterygidae 5 1 Cambaridae 5 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 97 % Contribution of Dominant Family: 40.21 % ( Heptageniidae ) Family Biotic Index: 5.22 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 45.36 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 112 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 12.5' / <1.0' Substrate: Sand, Silt, Snags....StreamBank Vegetation/Stability: Trees, Shrubs/Good Canopy: Partly Open....Other: Water temp. 22.3 C / pH 7.1 SU / DO 7.3 mg/L / Cond. 128 umhos; Agriculture-cropland & livestock, Forested Frogs present; ------

Station: AN0379 Millstone River, Route 33, Millstone Twp., Monmouth County Jamesburg USGS Quadrangle Date Sampled: 06/15/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 92 Tubificidae 10 4 BloodRed Chironomidae 8 3 Sphaeriidae 8 3 Sialidae 4 3 Cambaridae 5 2 Gomphidae 1 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 8 Total Number of Individuals: 109 % Contribution of Dominant Family: 84.40 % ( Chironomidae ) Family Biotic Index: 6.11 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 6 Biological Condition: Severely Impaired Habitat Analysis: 150 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Chironomidae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Slow....Width/Depth (ft): 12.0' / 2.5' Substrate: Mud....StreamBank Vegetation/Stability: Shrubs, Grass/Good Canopy: Open....Other: Water temp. 20.6 C / pH 7.2 SU / DO 6.5 mg/L / Cond. 158 umhos; Suburban site sampled between Rt 33 and adjacent to side road and U-turn exit; ------

Station: AN0380 Rocky Brook, Perrineville Rd., Millstone Twp., Monmouth County Roosevelt USGS Quadrangle Date Sampled: 09/16/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Sphaeriidae 8 26 Hydropsychidae 4 25 Chironomidae 6 23 Gammaridae 4 9 Leptoceridae 4 4 Planariidae 4 3 Coenagrionidae 9 3 Asellidae 8 3 Simuliidae 6 2 Philopotamidae 3 1 Glossiphoniidae 8 1 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 100 % Contribution of Dominant Family: 26.00 % ( Sphaeriidae ) Family Biotic Index: 5.84 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 30.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 148 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 37.0' / <1.0 - 2.0' Substrate: Cobbles, Gravel/Sand....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Fair Canopy: Mostly Closed....Other: Water Temp. 23.2 C / pH 7.0 SU / DO 7.3 mg/L / Cond. 170 umhos; Suburban. Weir upstream.; Fish and turtle present. ------

Station: AN0381 Rocky Brook, Main St., Hightstown Boro., Mercer Hightstown USGS Quadrangle Date Sampled: 09/14/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 38 Sphaeriidae 8 11 Hydropsychidae 4 8 Corbiculidae 8 7 Chironomidae 6 7 BloodRed Chironomidae 8 6 Naididae 7 5 Tetrastemmatidae 7 5 Planorbidae 6 4 Coenagrionidae 9 2 Planariidae 4 2 Hydrobiidae 8 1 Calopterygidae 5 1 Plagiostomidae 4 1 Physidae 7 1 Elmidae 4 1 ------Statistical Analysis ------Number of Taxa: 16 Total Number of Individuals: 100 % Contribution of Dominant Family: 38.00 % ( Gammaridae ) Family Biotic Index: 5.66 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 8.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 108 ( Marginal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 20.0'-25.0' / 1.0' Substrate: Cobbles, Gravel/Sand, Silt....StreamBank Vegetation/Stability: Trees, Shrubs/Fair Canopy: Partly Open....Other: Water Temp. 22.7 C / pH 7.0 SU / DO 8.3 mg/L / Cond. 231 umhos; Urban. Downstream of Peddie Lake outlet. Storm sewers present and flowing. ; Water fowl present. ------

Station: AN0382 Millstone River, Grovers Mill Rd., West Windsor Twp., Mercer/Middlesex County Hightstown USGS Quadrangle Date Sampled: 08/31/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 26 Coenagrionidae 9 18 Elmidae 4 14 Planorbidae 6 7 Physidae 7 6 Sphaeriidae 8 6 Tubificidae 10 4 Chironomidae 6 4 Glossiphoniidae 8 2 Gomphidae 1 2 Naididae 7 2 Corixidae 9 2 Hydrobiidae 8 1 Planariidae 4 1 Gerridae 8 1 Hydracarina 6 1 Hydroptilidae 4 1 Pleidae 9 1 BloodRed Chironomidae 8 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 100 % Contribution of Dominant Family: 26.00 % ( Gammaridae ) Family Biotic Index: 6.15 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 1.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 158 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear, grayish green....Flow: Slow....Width/Depth (ft): 77.0' / 2.0' - 3.0' Substrate: Sand, Mud....StreamBank Vegetation/Stability: Shrubs, Grass, Trees/Good Canopy: Mostly Open....Other: Water temp. 24.4 C / pH 7.2 SU / DO 5.8 mg/L / Cond. 281 umhos; Suburban, Forested minnows and crayfish present; ------

Station: AN0382B Millstone River, Rt. 535, East Windsor Twp., Mercer/Middlesex County Hightstown USGS Quadrangle Date Sampled: 09/14/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 41 Corbiculidae 8 13 Tubificidae 10 11 Elmidae 4 9 Chironomidae 6 7 Heptageniidae 4 4 BloodRed Chironomidae 8 3 Leptoceridae 4 3 Corixidae 9 2 Sphaeriidae 8 2 Tetrastemmatidae 7 2 Glossiphoniidae 8 1 Ancylidae 6 1 Calopterygidae 5 1 Tabanidae 6 1 Plagiostomidae 4 1 Coenagrionidae 9 1 Isotomidae 10 1 Planorbidae 6 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 105 % Contribution of Dominant Family: 39.05 % ( Gammaridae ) Family Biotic Index: 5.81 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 6.67 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 133 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Slow....Width/Depth (ft): 33.0' / 2.5' Substrate: Cobbles, Mud, Silt, Snags....StreamBank Vegetation/Stability: Trees, Shrubs/Fair Canopy: Partly Open....Other: Water temp. 21.0 C / pH 7.4 SU / DO 7.7 mg/L / Cond. 244 umhos; Suburban, forested ------

Station: AN0382D Millstone River, Applegarth Rd, Monroe Twp, Middlesex County Jamesburg USGS Quadrangle Date Sampled: 8/31/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------

Not Sampled due to bridge construction work

Station: AN0383 Big Bear Brook, Old Trenton Rd., West Windsor Twp., Mercer County Hightstown USGS Quadrangle Date Sampled: 08/31/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydrobiidae 8 34 Coenagrionidae 9 11 Elmidae 4 9 Chironomidae 6 8 Sphaeriidae 8 8 Physidae 7 6 Planorbidae 6 5 Tubificidae 10 3 Calopterygidae 5 3 Hydropsychidae 4 3 Naididae 7 2 Haliplidae 5 2 Corbiculidae 8 1 Culicidae 8 1 Lymnaeidae 6 1 BloodRed Chironomidae 8 1 Nepidae 8 1 Corduliidae 5 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 100 % Contribution of Dominant Family: 34.00 % ( Hydrobiidae ) Family Biotic Index: 7.15 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 3.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 160 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 29' 3" / 1.0'-1.5' Substrate: Gravel/Sand, Snags, Root Mats....StreamBank Vegetation/Stability: Grasses, Shrubs, Trees/Fair Canopy: Mostly Closed....Other: Water Temp. 24.9 C / pH 7.2 SU / DO 4.4 mg/L / Cond. 295 umhos; Agriculture-cropland and livestock, Suburban. Storm sewers present, Drainage pipe on right bank. ; Frogs, fish, filamentous algae present. ------

Station: AN0384 Bear Brook, Cranbury Rd., West Windsor Twp., Mercer County Hightstown USGS Quadrangle Date Sampled: 08/31/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 52 Hydropsychidae 4 14 Elmidae 4 11 Gammaridae 4 8 Naididae 7 6 Sphaeriidae 8 6 Simuliidae 6 4 Baetidae 4 3 Tubificidae 10 2 BloodRed Chironomidae 8 1 ------Statistical Analysis ------Number of Taxa: 10 Total Number of Individuals: 107 % Contribution of Dominant Family: 48.60 % ( Chironomidae ) Family Biotic Index: 5.59 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 15.89 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 170 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 10.0' / <1.0' - 1.5' Substrate: Gravel....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Fair Canopy: Mostly Closed....Other: Water temp. 22.7 C / pH 7.4 SU / DO 6.9 mg/ L / Cond. 191 umhos; Suburban, Forested storm sewers present; macrophytes, fish, and crayfish present ------

Station: AN0385 Cranbury Brook, Applegarth Rd., Monroe Twp., Middlesex County Jamesburg USGS Quadrangle Date Sampled: 09/14/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 66 Tubificidae 10 21 BloodRed Chironomidae 8 5 Asellidae 8 2 Coenagrionidae 9 2 Naididae 7 2 Sphaeriidae 8 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 8 Total Number of Individuals: 100 % Contribution of Dominant Family: 66.00 % ( Chironomidae ) Family Biotic Index: 7.09 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 3 Biological Condition: Severely Impaired Habitat Analysis: 146 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Chironomidae Family Overwhelmingly Dominant - - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Slow....Width/Depth (ft): 18.0' / 1.0'-2.0' Substrate: Gravel/Sand, Silt....StreamBank Vegetation/Stability: Trees, Shrubs/Fair Canopy: Mostly Closed....Other: Water Temp. 17.4 C / pH 6.9 SU / DO 8.5 mg/L / Cond. 199 umhos; Agriculture-cropland, Forested. Storm sewers present, recent work on storm sewers upstream.; Macrophytes, filamentous algae, frogs present. ------

Station: AN0386 Cranbury Brook, Maple Ave., Plainsboro Twp. , Middlesex County Hightstown USGS Quadrangle Date Sampled: 08/31/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 59 BloodRed Chironomidae 8 19 Naididae 7 15 Gammaridae 4 3 Coenagrionidae 9 3 Hydropsychidae 4 2 Planariidae 4 2 Hydroptilidae 4 2 Tubificidae 10 1 ------Statistical Analysis ------Number of Taxa: 9 Total Number of Individuals: 106 % Contribution of Dominant Family: 55.66 % ( Chironomidae ) Family Biotic Index: 6.45 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 3.77 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 158 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Slow....Width/Depth (ft): 31.0' / 2.0' - 3.0' Substrate: Gravel/Sand, Mud....StreamBank Vegetation/Stability: Shrubs, Trees/Fair Canopy: Partly Open....Other: Water temp. 24.6 C / pH 7.3 SU / DO 6.3 mg/L / Cond. 188 umhos; Suburban, Forested banks stabilized with rip rap and fencing; fish and water snakes present ------

Station: AN0387 Devils Brook, New Rd., South Brunswick Twp., Middlesex County County Hightstown USGS Quadrangle Date Sampled: 09/16/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Asellidae 8 30 Chironomidae 6 25 Sphaeriidae 8 15 Hydropsychidae 4 9 Tubificidae 10 9 Gerridae 8 3 Glossiphoniidae 8 2 Caenidae 7 1 Elmidae 4 1 Gyrinidae 3 1 Dytiscidae 5 1 Physidae 7 1 BloodRed Chironomidae 8 1 Corixidae 9 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 100 % Contribution of Dominant Family: 30.00 % ( Asellidae ) Family Biotic Index: 7.19 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 10.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 166 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 10.0' / 0.5' - 2.5' Substrate: Gravel, Sand, Silt, Snags....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Good Canopy: Closed....Other: Water Temp. 19.3 C / pH 6.5 SU / DO 5.2 mg/L / Cond. 111 umhos; Suburban. ------

Station: AN0388 Shallow Brook, Scotts Corner Rd., South Brunswick Twp., Middlesex County Hightstown USGS Quadrangle Date Sampled: 09/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------BloodRed Chironomidae 8 24 Coenagrionidae 9 22 Tubificidae 10 13 Chironomidae 6 10 Asellidae 8 10 Talitridae 8 7 Caenidae 7 3 Libellulidae 9 3 Planariidae 4 3 Hydrophilidae 5 1 Ceratopogonidae 6 1 Elmidae 4 1 Glossiphoniidae 8 1 Erpobdellidae 8 1 Haliplidae 5 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 101 % Contribution of Dominant Family: 23.76 % ( BloodRed Chironomidae ) Family Biotic Index: 8.04 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 2.97 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 146 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Slow....Width/Depth (ft): 17.0' / 2.0' Substrate: Gravel/Sand, Mud....StreamBank Vegetation/Stability: Trees, Grass/Good Canopy: Mostly Open....Other: Water Temp. 18.3 C / pH 6.6 SU / DO 6.3 mg/L / Cond. 146 umhos; Suburban. Storm sewers present. Downstream of beaver dam. Macrophytes present.; Houses on left bank, high tension lines crossing stream. ------

Station: AN0389 Devils Bk, Schalks Crossing Rd (Rt 683), Plainsboro Twp, Middlesex County Hightstown USGS Quadrangle Date Sampled: 9/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------

Not Sampled due to bridge construction work

Station: AN0390 Camp Harmony Br. Of Stony Bk., Vandyke Rd., Hopewell Twp., Mercer County Hopewell USGS Quadrangle Date Sampled: 09/09/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Sphaeriidae 8 29 Hydropsychidae 4 16 Chironomidae 6 9 Philopotamidae 3 7 Elmidae 4 7 Psephenidae 4 5 Baetidae 4 4 Calopterygidae 5 3 Tipulidae 3 3 Dixidae 3 2 Talitridae 8 2 Tubificidae 10 2 Belostomatidae 9 1 Aeshnidae 3 1 Asellidae 8 1 BloodRed Chironomidae 8 1 Naididae 7 1 Gerridae 8 1 Dytiscidae 5 1 Lumbriculidae 8 1 Veliidae 9 1 Leptophlebiidae 2 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 23 Total Number of Individuals: 100 % Contribution of Dominant Family: 29.00 % ( Sphaeriidae ) Family Biotic Index: 5.72 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 29.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 162 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 30.0' / <1.0'-1.5' Substrate: Cobbles, Gravel....StreamBank Vegetation/Stability: Grasses, Trees, Shrubs/Fair Canopy: Partly Open....Other: Water Temp. 21.3 C / pH 7.2 SU / DO 7.2 mg/L / Cond. 206 umhos; Rural, Forested. Macrophytes, filamentous algae, crayfish, and fish present.; ------

Station: AN0391 Stony Brook, Mine Rd., Hopewell Twp., Mercer County Pennington USGS Quadrangle Date Sampled: 09/09/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 19 Psephenidae 4 17 Gammaridae 4 11 Baetidae 4 10 Heptageniidae 4 10 Chironomidae 6 8 Asellidae 8 4 Philopotamidae 3 4 Planariidae 4 4 Veliidae 9 3 Planorbidae 6 2 Elmidae 4 1 Coenagrionidae 9 1 Calopterygidae 5 1 Corydalidae 0 1 BloodRed Chironomidae 8 1 Leptophlebiidae 2 1 Naididae 7 1 Gomphidae 1 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 100 % Contribution of Dominant Family: 19.00 % ( Hydropsychidae ) Family Biotic Index: 4.51 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 44.00 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 167 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 51.0' / 1.0' - 1.5' Substrate: Cobbles, Gravel....StreamBank Vegetation/Stability: Grasses, Trees, Shrubs/Good Canopy: Mostly Open....Other: Water temp. 18.5 C / pH 7.3 SU / DO 6.3 mg/L / Cond. 240 umhos; Agriculture- livestock (horses) open grated bridge; crayfish and macrophytes present ------

Station: AN0392 Stony Brook, Old Mill Rd., Pennington Boro, Mercer Pennington USGS Quadrangle Date Sampled: 09/09/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 18 Philopotamidae 3 15 Elmidae 4 15 Hydropsychidae 4 8 Planariidae 4 7 Gammaridae 4 6 Sphaeriidae 8 6 Heptageniidae 4 6 Psephenidae 4 4 Asellidae 8 3 Culicidae 8 2 Baetidae 4 2 Coenagrionidae 9 1 Oligoneuriidae 2 1 Lumbriculidae 8 1 Erpobdellidae 8 1 Naididae 7 1 Planorbidae 6 1 BloodRed Chironomidae 8 1 Veliidae 9 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 100 % Contribution of Dominant Family: 18.00 % ( Chironomidae ) Family Biotic Index: 4.90 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 32.00 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 154 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 66.0' / 1.0'-1.5' Substrate: Cobbles, Gravel....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Fair Canopy: Mostly Open....Other: Water Temp. 23.4 C / pH 7.1 SU / DO 6.6 mg/L / Cond. 423 umhos; Agriculture-cropland (cornfield), Rural Minnows and crayfish present.; ------

Station: AN0393 Stony Brook, Rt. 206, Princeton Twp., Mercer County Princeton USGS Quadrangle Date Sampled: 09/09/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Elmidae 4 16 Chironomidae 6 14 Hydrobiidae 8 12 Psephenidae 4 12 Hydropsychidae 4 10 Philopotamidae 3 10 Physidae 7 5 Heptageniidae 4 4 Pleuroceridae 6 3 Gammaridae 4 3 Planorbidae 6 3 Aeshnidae 3 2 Simuliidae 6 2 Baetidae 4 1 Erpobdellidae 8 1 Sphaeriidae 8 1 Naididae 7 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 100 % Contribution of Dominant Family: 16.00 % ( Elmidae ) Family Biotic Index: 5.06 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 25.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 173 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 56.0' / <1.0'-1.0' Substrate: Cobbles, Gravel....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Good Canopy: Mostly Open....Other: Water Temp. 24.7 C / pH 7.2 SU / DO 7.0 mg/L / Cond. 314 umhos; Suburban. Downstream of weir, USGS gauge present.; Crayfish, minnows, macrophytes, water snake present. ------

Station: AN0394 Duck Pond Run, Route 1, West Windsor Twp., Mercer County Princeton USGS Quadrangle Date Sampled: 09/16/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Tubificidae 10 45 Chironomidae 6 20 Gammaridae 4 11 Asellidae 8 7 BloodRed Chironomidae 8 7 Sphaeriidae 8 3 Glossiphoniidae 8 2 Culicidae 8 1 Corixidae 9 1 Elmidae 4 1 Plagiostomidae 4 1 Phryganeidae 4 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 100 % Contribution of Dominant Family: 45.00 % ( Tubificidae ) Family Biotic Index: 7.95 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 1.00 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 141 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Slow....Width/Depth (ft): 27.0' / 3.0' Substrate: Mud, Silt, Snags....StreamBank Vegetation/Stability: Trees, Grass, Shrubs/Fair Canopy: Mostly Closed....Other: Water Temp. 19.0 C / pH 6.5 SU / DO 6.9 mg/L / Cond. 223 umhos; Urban. Storm sewers present.; Organic matter/leaf litter on bottom. ------

Station: AN0395 Heathcote Brook, Stouts Ln., South Brunswick Twp., Middlesex County Monmouth Jct USGS Quadrangle Date Sampled: 09/16/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Tubificidae 10 38 Sphaeriidae 8 14 Hydropsychidae 4 9 Gammaridae 4 7 Hydrobiidae 8 6 BloodRed Chironomidae 8 4 Naididae 7 4 Elmidae 4 4 Chironomidae 6 4 Asellidae 8 3 Coenagrionidae 9 2 Planorbidae 6 2 Glossiphoniidae 8 1 Physidae 7 1 Dendrocoelidae 4 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 38.00 % ( Tubificidae ) Family Biotic Index: 7.77 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 9.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 126 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Slow....Width/Depth (ft): 11.0' / < 1.0' Substrate: Gravel, Mud, Silt....StreamBank Vegetation/Stability: Grasses, Trees, Shrubs/Poor Canopy: Closed....Other: Water temp. 20.1 C / pH 6.8 SU / DO 4.4 mg/L / Cond. 405 umhos; Suburban, forested two stream confluences, one on each bank; fish present ------

Station: AN0396 Heathcote Brook, Academy St., South Brunswick Twp., Middlesex County Hightstown USGS Quadrangle Date Sampled: 09/09/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 34 Chironomidae 6 33 Naididae 7 6 Hydrobiidae 8 5 Elmidae 4 5 Hydropsychidae 4 4 BloodRed Chironomidae 8 3 Philopotamidae 3 2 Corixidae 9 2 Tubificidae 10 2 Planorbidae 6 2 Sphaeriidae 8 2 Planariidae 4 1 Leptoceridae 4 1 Nematoda 6 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 103 % Contribution of Dominant Family: 33.01 % ( Gammaridae ) Family Biotic Index: 5.46 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 6.80 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 168 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 51.0' / <1.0'-1.5' Substrate: Gravel/ Sand, Mud....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Good Canopy: Partly Open....Other: Water Temp. 21.0 C / pH 7.2 SU / DO 6.6 mg/L / Cond. 311 umhos; Suburban. Macrophytes, crayfish, eels, fish, and periphyton present.; ------

Station: AN0397 Millstone River, Outlet Of Carnegie Lake, Route 27, South Brunswick Twp., Middlesex & Mercer County Hightstown USGS Quadrangle Date Sampled: 09/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 27 Hydropsychidae 4 13 Corbiculidae 8 11 Gammaridae 4 9 BloodRed Chironomidae 8 9 Elmidae 4 9 Sphaeriidae 8 8 Naididae 7 5 Planariidae 4 4 Tubificidae 10 3 Pleuroceridae 6 2 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 100 % Contribution of Dominant Family: 27.00 % ( Chironomidae ) Family Biotic Index: 6.03 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 13.00 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 170 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Fast....Width/Depth (ft): 31.0' / 2.0' Substrate: Cobbles, Gravel/Sand....StreamBank Vegetation/Stability: Trees, Shrubs/Good Canopy: Partly Open....Other: Water Temp. 19.4 C / pH 7.6 SU / DO 8.7 mg/L / Cond. 296 umhos; Suburban, Forested, D&R Canal Park. Station downstream of Carnegie Lake.; ------

Station: AN0398 Bedens Brook, Aunt Molly Rd., Hopewell Twp., Mercer County Rocky Hill USGS Quadrangle Date Sampled: 09/09/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 13 Coenagrionidae 9 12 Physidae 7 12 BloodRed Chironomidae 8 10 Hydropsychidae 4 8 Tubificidae 10 8 Hydrophilidae 5 7 Gammaridae 4 5 Sphaeriidae 8 5 Planorbidae 6 4 Planariidae 4 3 Elmidae 4 3 Glossiphoniidae 8 3 Haliplidae 5 2 Corixidae 9 1 Dytiscidae 5 1 Psephenidae 4 1 Naididae 7 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 100 % Contribution of Dominant Family: 13.00 % ( Chironomidae ) Family Biotic Index: 6.68 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 9.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 165 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Slow....Width/Depth (ft): 15.0' / 1.0' - 3.0' Substrate: Cobbles, Gravel, Shale....StreamBank Vegetation/Stability: Grasses, Shrubs, Trees/Good Canopy: Mostly Open....Other: Water Temp. 22.5 C / pH 7.3 SU / DO 6.1 mg/L / Cond. 228 umhos; Agriculture - cropland and livestock. Upstream of sewage pumping station.; Algae, macrophytes, fish, tadpoles, crayfish, salamanders present. ------

Station: AN0399 Rock Brook, Long Hill Rd., Montgomery Twp., Somerset County Rocky Hill USGS Quadrangle Date Sampled: 09/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 37 Psephenidae 4 20 Chironomidae 6 16 Philopotamidae 3 7 Elmidae 4 6 Perlidae 1 3 Tipulidae 3 3 Glossosomatidae 0 2 Naididae 7 2 Baetidae 4 1 Empididae 6 1 Lumbricidae 10 1 Veliidae 9 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 100 % Contribution of Dominant Family: 37.00 % ( Hydropsychidae ) Family Biotic Index: 4.24 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 50.00 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 171 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 27.0' / <1.0 ' Substrate: Cobbles, Gravel/Sand....StreamBank Vegetation/Stability: Trees, Shrubs/Good Canopy: Partly Open....Other: Water Temp. 17.8 C / pH 7.7 SU / DO 8.3 mg/L / Cond. 181 umhos; Suburban, Forested. Fish present.; ------

Station: AN0400 Rock Brook, Burnt Mill Rd., Montgomery Twp., Somerset County Rocky Hill USGS Quadrangle Date Sampled: 09/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 40 Philopotamidae 3 16 Heptageniidae 4 10 Psephenidae 4 6 Chironomidae 6 6 Caenidae 7 4 Elmidae 4 4 Baetidae 4 2 Planariidae 4 2 Lumbriculidae 8 2 Tubificidae 10 1 Asellidae 8 1 Corixidae 9 1 Gammaridae 4 1 Sphaeriidae 8 1 Tetrastemmatidae 7 1 Simuliidae 6 1 Libellulidae 9 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 100 % Contribution of Dominant Family: 40.00 % ( Hydropsychidae ) Family Biotic Index: 4.45 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 72.00 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 128 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 78.0' / 1.0' Substrate: Cobbles, Silt....StreamBank Vegetation/Stability: Trees, Grass/Fair Canopy: Mostly Open....Other: Water Temp. 19.5 C / pH 7.8 SU / DO 8.3 mg/L / Cond. 235 umhos; Suburban. Fish and frogs present.; ------

Station: AN0401 Bedens Brook, Route 206, Montgomery Twp., Somerset County Rocky Hill USGS Quadrangle Date Sampled: 09/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Heptageniidae 4 36 Elmidae 4 19 Philopotamidae 3 12 Hydropsychidae 4 11 Chironomidae 6 6 Planariidae 4 3 Gammaridae 4 3 Baetidae 4 1 Hydrophilidae 5 1 Planorbidae 6 1 Erpobdellidae 8 1 BloodRed Chironomidae 8 1 Sphaeriidae 8 1 Polycentropodidae 6 1 Tetrastemmatidae 7 1 Psephenidae 4 1 Veliidae 9 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 100 % Contribution of Dominant Family: 36.00 % ( Heptageniidae ) Family Biotic Index: 4.25 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 61.00 NJIS Rating: 27 Biological Condition: Nonimpaired Habitat Analysis: 143 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 56.0' / <1.0' Substrate: Cobbles, Gravel/Sand....StreamBank Vegetation/Stability: Trees, Shrubs/Poor Canopy: Mostly Closed....Other: Water Temp. 18.3 C / pH 7.6 SU / DO 7.8 mg/L / Cond. 320 umhos; Suburban, Forested. Fish and macrophytes present.; ------

Station: AN0402 Pike Run, Route 206, Montgomery Twp., Somerset County Rocky Hill USGS Quadrangle Date Sampled: 09/30/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 22 Elmidae 4 20 Sphaeriidae 8 11 Hydropsychidae 4 8 Corixidae 9 7 Heptageniidae 4 7 Psephenidae 4 6 Calopterygidae 5 4 Philopotamidae 3 4 Chironomidae 6 3 Leptoceridae 4 3 Veliidae 9 2 Baetidae 4 1 Plagiostomidae 4 1 Gomphidae 1 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 22.00 % ( Gammaridae ) Family Biotic Index: 4.92 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 23.00 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 161 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 29.0' / <1.0' - 2.0' Substrate: Gravel/Sand, Mud, Snags ....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Fair Canopy: Partly Open....Other: Water temp. 16.7 C / pH 7.5 SU / DO 8.4 mg/L / Cond. 201 umhos; Suburban small flowing tributary on right bank; fish present; upstream blocked by fallen trees, causing stream to pool ------

Station: AN0403 Cruser Brook, Route 206, Montgomery Twp., Somerset County Rocky Hill USGS Quadrangle Date Sampled: 09/30/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 26 Gammaridae 4 19 BloodRed Chironomidae 8 15 Elmidae 4 14 Tipulidae 3 7 Tubificidae 10 6 Caenidae 7 3 Corixidae 9 3 Chironomidae 6 3 Physidae 7 2 Psephenidae 4 2 Heptageniidae 4 2 Baetidae 4 1 Empididae 6 1 Plagiostomidae 4 1 Coenagrionidae 9 1 Planorbidae 6 1 Sphaeriidae 8 1 Sialidae 4 1 Corduliidae 5 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 110 % Contribution of Dominant Family: 23.64 % ( Hydropsychidae ) Family Biotic Index: 5.26 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 29.09 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 153 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Turbid, grayish green....Flow: Moderate....Width/Depth (ft): 59.0' / 2.5' Substrate: Gravel, Silt....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Poor Canopy: Partly Open....Other: Water temp. 16.2 C / pH 8.0 SU / DO 9.0 mg/L / Cond. 216 umhos; Suburban side channel flowing in on left bank; fish present ------

Station: AN0404 Back Brook, Rt. 206, Montgomery Twp., Somerset County Rocky Hill USGS Quadrangle Date Sampled: 09/21/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Elmidae 4 25 Chironomidae 6 21 Sphaeriidae 8 11 Heptageniidae 4 11 Hydrobiidae 8 6 Tubificidae 10 6 Hydropsychidae 4 5 Asellidae 8 4 Psephenidae 4 4 Coenagrionidae 9 3 Naididae 7 3 Tetrastemmatidae 7 3 Planariidae 4 2 Gomphidae 1 2 Philopotamidae 3 1 Nematoda 6 1 Leptoceridae 4 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 109 % Contribution of Dominant Family: 22.94 % ( Elmidae ) Family Biotic Index: 5.74 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 16.51 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 121 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 30.0' / <1.0' Substrate: Gravel/Sand....StreamBank Vegetation/Stability: Trees/Poor Canopy: Mostly Closed....Other: Water Temp. 16.4 C / pH 7.3 SU / DO 7.5 mg/L / Cond. 380 umhos; Suburban. Fish present.; ------

Station: AN0405 Pike Run, Route 533, Montgomery Twp., Somerset County Rocky Hill USGS Quadrangle Date Sampled: 09/23/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 33 Chironomidae 6 25 Elmidae 4 13 Hydrobiidae 8 3 Asellidae 8 3 Coenagrionidae 9 3 BloodRed Chironomidae 8 3 Heptageniidae 4 3 Tubificidae 10 2 Hydrophilidae 5 2 Baetidae 4 2 Sialidae 4 2 Libellulidae 9 2 Corixidae 9 1 Psephenidae 4 1 Planorbidae 6 1 Physidae 7 1 Sphaeriidae 8 1 Naididae 7 1 Veliidae 9 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 103 % Contribution of Dominant Family: 32.04 % ( Gammaridae ) Family Biotic Index: 5.43 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 4.85 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 150 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 40 / <1.0 Substrate: Gravel / Silt....StreamBank Vegetation/Stability: Trees / Shrubs/Fair Canopy: Mostly Closed....Other: Water Temp 20.1C / pH 7.7SU / DO 8.1mg/L / Cond 450umhos; Suburban / storm sewers present / small ditch drains on right bank / banks very eroded downstream / recent bridge; construction / periphyton, fish, crayfish present. ------

Station: AN0406 Simonson Bk, Canal Road, Franklin Twp., Somerset County Monmouth Jct. USGS Quadrangle Date Sampled: 09/23/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 37 Physidae 7 24 Naididae 7 17 Gammaridae 4 9 BloodRed Chironomidae 8 4 Elmidae 4 3 Baetidae 4 2 Psephenidae 4 2 Lymnaeidae 6 1 Leptoceridae 4 1 ------Statistical Analysis ------Number of Taxa: 10 Total Number of Individuals: 100 % Contribution of Dominant Family: 37.00 % ( Chironomidae ) Family Biotic Index: 6.15 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 3.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 112 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 4.0 / <1.0 Substrate: Cobbles/ Gravel/ Sand....StreamBank Vegetation/Stability: Grass/ Trees/ Shrubs/Fair Canopy: Partly Open....Other: Water temp. 24.9C / pH 7.5 SU / DO 6.9mg/L / Cond 199 umhos; Suburban / very slow flow, if at all / puddled / ponded & algae overgrown downstream / gravel "dam" present.; ------

Station: AN0407 Ten Mile Run, Canal Road, Franklin Twp., Somerset County Monmouth Jct. USGS Quadrangle Date Sampled: 09/23/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 48 Hydropsychidae 4 12 Psephenidae 4 9 Philopotamidae 3 5 Tanyderidae 5 5 Gammaridae 4 4 Veliidae 9 4 Naididae 7 4 Heptageniidae 4 4 Elmidae 4 4 Tipulidae 3 3 BloodRed Chironomidae 8 2 Ancylidae 6 1 Coenagrionidae 9 1 Baetidae 4 1 Chironomidae 6 1 Tetrastemmatidae 7 1 Libellulidae 9 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 110 % Contribution of Dominant Family: 43.64 % ( Chironomidae ) Family Biotic Index: 5.36 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 20.00 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 155 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 12.0/<1.0-2.0 Substrate: Gravel/ Mud....StreamBank Vegetation/Stability: Trees/ Shrubs/ Grass/Good Canopy: Mostly Closed....Other: Water temp 19.4 C / pH 7.7 SU / DO 7.8 mg/L / Cond 407 umhos; Agriculture / Rural fish present; ------

Station: AN0408 Six Mile Run, Route 27, Franklin Twp., Somerset & Middlesex County Monmouth Junction USGS Quadrangle Date Sampled: 10/05/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Coenagrionidae 9 43 Gammaridae 4 24 Sphaeriidae 8 17 Hydropsychidae 4 9 Tetrastemmatidae 7 3 Calopterygidae 5 2 Tubificidae 10 2 Curculionidae 7 1 Planariidae 4 1 BloodRed Chironomidae 8 1 Erpobdellidae 8 1 Planorbidae 6 1 Elmidae 4 1 Libellulidae 9 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 107 % Contribution of Dominant Family: 40.19 % ( Coenagrionidae ) Family Biotic Index: 7.03 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 8.41 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 149 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 37.0' / <1.0' - 2.0' Substrate: Cobbles, Gravel/Sand, Snags....StreamBank Vegetation/Stability: Trees, Shrubs/Fair Canopy: Closed....Other: Water Temp. 15.4 C / pH 6.9 SU / DO 6.9 mg/L / Cond. 343 umhos; Agriculture-cropland, Suburban. Storm sewers present and flowing. ; Sewage odor. ------

Station: AN0409 Six Mile Run, Canal Road, Franklin Twp., Somerset County Monmouth Jct. USGS Quadrangle Date Sampled: 09/23/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 48 Chironomidae 6 38 Hydrobiidae 8 9 Elmidae 4 2 Asellidae 8 2 Hydropsychidae 4 2 Baetidae 4 1 Calopterygidae 5 1 Lepidostomatidae 1 1 Chironomidae 6 1 Physidae 7 1 Tubificidae 10 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 107 % Contribution of Dominant Family: 44.86 % ( Gammaridae ) Family Biotic Index: 5.21 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 3.74 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 140 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 40.0/ <1.0-1.5 Substrate: Gravel/ Silt....StreamBank Vegetation/Stability: Trees/ Shrubs/ Grass/Fair Canopy: Mostly Closed....Other: Water temp 17.2C / pH 7.5 SU / DO 8.2mg/L / Cond 316 umhos; Rural, forested / orange, slimy residue along right bank / frogs and fish present; ------

Station: AN0410 Millstone River, Blackwells Mills Road, Hillsboro Twp., Somerset County Monmouth Jct. USGS Quadrangle Date Sampled: 09/23/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Asellidae 8 25 Tubificidae 10 19 Elmidae 4 13 Gammaridae 4 11 Chironomidae 6 11 Hydrobiidae 8 7 BloodRed Chironomidae 8 3 Pleuroceridae 6 3 Heptageniidae 4 3 Corbiculidae 8 2 Polycentropodidae 6 2 Coenagrionidae 9 1 Nematoda 6 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 102 % Contribution of Dominant Family: 24.51 % ( Asellidae ) Family Biotic Index: 6.98 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 4.90 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 157 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 100.0 / 3.0 Substrate: Gravel / sand, mud....StreamBank Vegetation/Stability: Trees / Shrubs /Good Canopy: Open....Other: Water temp. 19.6 C / pH 7.2 SU / DO 7.1mg/L / Cond 387 umhos; Suburban adjacent to small park or pull off area; upstream of USGS gauge ------

Station: AN0411 Royce Brook, Route 206, Hillsborough Twp., Somerset County Rocky Hill USGS Quadrangle Date Sampled: 09/30/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 47 Elmidae 4 19 Hydropsychidae 4 14 Philopotamidae 3 7 Coenagrionidae 9 4 Corbiculidae 8 2 Veliidae 9 2 Tipulidae 3 1 Chironomidae 6 1 Planariidae 4 1 Ancylidae 6 1 Naididae 7 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 100 % Contribution of Dominant Family: 47.00 % ( Gammaridae ) Family Biotic Index: 4.37 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 21.00 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 160 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 36.0' / <1.0' - 2.5' Substrate: Cobbles, Gravel, Mud....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Fair Canopy: Mostly Closed....Other: Water Temp. 18.0 C / pH 7.6 SU / DO 7.9 mg/L / Cond. 228 umhos; Suburban. Two storm sewers flowing. ; Light Rain. Fish present. ------

Station: AN0412 Royce Brook Branch, Route 206, Hillsborough Twp., Somerset County Raritan USGS Quadrangle Date Sampled: 09/30/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 78 Hydropsychidae 4 5 Chironomidae 6 4 Calopterygidae 5 3 BloodRed Chironomidae 8 3 Enchytraeidae 10 3 Coenagrionidae 9 2 Baetidae 4 1 Naididae 7 1 Gerridae 8 1 Lumbricidae 10 1 Planorbidae 6 1 Physidae 7 1 Sphaeriidae 8 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 106 % Contribution of Dominant Family: 73.58 % ( Gammaridae ) Family Biotic Index: 4.72 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 5.66 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 162 ( Optimal ) USEPA Protocol Deficiency(s) noted: Gammaridae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 25.0' / 2.5' Substrate: Cobbles, Gravel/sand, Snags....StreamBank Vegetation/Stability: Grasses, Trees, Shrubs/Fair Canopy: Partly Open....Other: Water temp. 18.5 C / pH 7.7 SU / DO 7.9 mg/L / Cond. 189 umhos; Suburban ------

Station: AN0413 Royce Brook, Rt. 533, Manville Boro, Somerset County Bound Brook USGS Quadrangle Date Sampled: 09/23/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------BloodRed Chironomidae 8 33 Tubificidae 10 26 Chironomidae 6 19 Gammaridae 4 14 Culicidae 8 5 Elmidae 4 4 Tetrastemmatidae 7 2 Ancylidae 6 1 Glossiphoniidae 8 1 Lumbriculidae 10 1 ------Statistical Analysis ------Number of Taxa: 10 Total Number of Individuals: 106 % Contribution of Dominant Family: 31.13 % ( BloodRed Chironomidae ) Family Biotic Index: 7.43 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 131 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 58.0' / <1.0' - 1.5' Substrate: Gravel/Sand....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Fair Canopy: Mostly Closed....Other: Water temp. 18.6 C / pH 7.5 SU / DO 8.7 mg/L / Cond. 263 umhos; Urban; much litter present storm sewers present; periphyton, ducks, fish present; sheen on surface, gasoline smell ------

Station: AN0414 Millstone River, Abv. Raritan Confl., Manville Boro, Somerset County Bound Brook USGS Quadrangle Date Sampled: 10/07/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 35 Heptageniidae 4 13 Asellidae 8 11 Sphaeriidae 8 8 Elmidae 4 5 Hydrobiidae 8 5 Valvatidae 4 5 Chironomidae 6 3 Planorbidae 6 3 BloodRed Chironomidae 8 3 Coenagrionidae 9 2 Tubificidae 10 2 Philopotamidae 3 1 Tabanidae 6 1 Erpobdellidae 8 1 Leptoceridae 4 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 17 Total Number of Individuals: 100 % Contribution of Dominant Family: 35.00 % ( Gammaridae ) Family Biotic Index: 5.50 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 15.00 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 123 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 200' / >4.0' Substrate: Gravel/Sand, Mud....StreamBank Vegetation/Stability: Trees, Shrubs/Good Canopy: Open....Other: Water Temp. 15.0 C / pH 8.0 SU / DO 7.0 mg/L / Cond. 233 umhos; Suburban Upstream of pumping facility. Recent flooding. ; Macrophytes present ------

Station: AN0415 Cuckles Brook, Rt 28 (E. Main St), Bridgewater Twp, Somerset County Bound Brook USGS Quadrangle Date Sampled: 06/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------BloodRed Chironomidae 8 26 Chironomidae 6 23 Coenagrionidae 9 19 Physidae 7 18 Naididae 7 6 Hydropsychidae 4 3 Aeshnidae 3 2 Calopterygidae 5 1 Corduliidae 5 1 Tubificidae 10 1 Planorbidae 6 1 Haliplidae 5 1 Sphaeriidae 8 1 Veliidae 9 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 105 % Contribution of Dominant Family: 24.76 % ( BloodRed Chironomidae ) Family Biotic Index: 7.18 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 2.86 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 134 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid, grayish color....Flow: Moderate....Width/Depth (ft): 8 ' / < 1.0 ' Substrate: Gravel....StreamBank Vegetation/Stability: Trees, shrubs, grasses/Fair Canopy: Closed....Other: Water temp. 18.6 C /pH 7.1 SU /DO 6.3 mg/L /Cond 635 umhos; Suburban / light industrial (adj to Recycling facility) 3 storm drains on right bank; fathead and other minnows, trash ------

Station: AN0416 West Branch Middle Brook, Crim Road, Bridgewater Twp, Somerset County Bound Brook USGS Quadrangle Date Sampled: 06/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 64 BloodRed Chironomidae 8 8 Simuliidae 6 5 Gammaridae 4 4 Baetidae 4 3 Planariidae 4 3 Naididae 7 3 Elmidae 4 3 Aeshnidae 3 1 Tipulidae 3 1 Coenagrionidae 9 1 Tubificidae 10 1 Lumbriculidae 8 1 Psephenidae 4 1 Gerridae 8 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 64.00 % ( Chironomidae ) Family Biotic Index: 5.96 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 3.00 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 158 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Chironomidae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear, grayish color....Flow: Slow....Width/Depth (ft): 10' / 1-2' Substrate: Cobbles, Gravel....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Good Canopy: Mostly Closed....Other: Water temp. 16.9 C / pH 7.6 SU / DO 7.7 mg/L / Cond. 320 umhos; Suburban, Forested Storm Sewers present and flowing; Fish present, USGS flood monitoring sensor # 1570 present ------

Station: AN0417 West Branch Middle Brook, Chimney Rock Rd, Bridgewater Twp, Somerset County Bound Brook USGS Quadrangle Date Sampled: 05/25/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 47 Simuliidae 6 15 Hydropsychidae 4 13 Gammaridae 4 6 BloodRed Chironomidae 8 4 Elmidae 4 4 Coenagrionidae 9 2 Caenidae 7 2 Physidae 7 2 Aeshnidae 3 1 Asellidae 8 1 Leptoceridae 4 1 Naididae 7 1 Psephenidae 4 1 Pteronarcyidae 0 1 Sphaeriidae 8 1 ------Statistical Analysis ------Number of Taxa: 16 Total Number of Individuals: 102 % Contribution of Dominant Family: 46.08 % ( Chironomidae ) Family Biotic Index: 5.65 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 16.67 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 152 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 32 ' / < 1.0' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, weeds/Fair Canopy: Mostly Open....Other: Water temp. 25.8 C /pH 7.8 SU /DO 7.0 mg/L Cond 255 umhos; Forested; downstream of reservoir yoy minnows; ------

Station: AN0418 E Br. Middle Brook, Top Of World Way (Formerly Green Valley Rd), Warren Twp, Somerset County Bound Brook USGS Quadrangle Date Sampled: 06/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Simuliidae 6 38 Gammaridae 4 25 Asellidae 8 9 Planariidae 4 6 Chironomidae 6 6 Elmidae 4 5 Hydropsychidae 4 4 Psephenidae 4 3 Tubificidae 10 1 Baetidae 4 1 Physidae 7 1 Naididae 7 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 100 % Contribution of Dominant Family: 38.00 % ( Simuliidae ) Family Biotic Index: 5.36 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 5.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 157 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Fast....Width/Depth (ft): 5 ' / <= 1.0 ' Substrate: Cobbles....StreamBank Vegetation/Stability: Shrubs, trees, grasses/Good Canopy: Mostly Open....Other: Water temp. 18.6 C /pH 7.4 SU /DO 6.8 mg/L /Cond 500 umhos; Suburban frogs, fish, crayfish, salamanders; ------

Station: AN0419 E Br Middle Brook, Gilbride Rd, Bridgewater Twp, Somerset County Bound Brook USGS Quadrangle Date Sampled: 05/25/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 42 Elmidae 4 27 Psephenidae 4 12 Hydropsychidae 4 6 Naididae 7 6 Baetidae 4 5 Gammaridae 4 2 Philopotamidae 3 1 Cambaridae 5 1 BloodRed Chironomidae 8 1 ------Statistical Analysis ------Number of Taxa: 10 Total Number of Individuals: 103 % Contribution of Dominant Family: 40.78 % ( Chironomidae ) Family Biotic Index: 5.03 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 11.65 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 157 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 29 ' / 1-2 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, weeds/Fair Canopy: Mostly Open....Other: Water temp. 20.9 C /pH 7.7 SU /DO 8.6 mg/L /Cond 355 umhos; Forested (Washington Valley Park) rip rap stone wall by bridge; minnows, fish, frogs, water snake ------

Station: AN0420 Middle Brook, Rt 28 (Talmidge Rd), Bridgewater Twp, Somerset County Bound Brook USGS Quadrangle Date Sampled: 06/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 28 Naididae 7 24 Chironomidae 6 12 Hydropsychidae 4 9 Baetidae 4 8 Gammaridae 4 5 Tipulidae 3 2 Asellidae 8 2 Hydrophilidae 5 2 Veliidae 9 2 Philopotamidae 3 1 Collembola 10 1 Corixidae 9 1 Coenagrionidae 9 1 BloodRed Chironomidae 8 1 Physidae 7 1 Tetrastemmatidae 7 1 Simuliidae 6 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 19 Total Number of Individuals: 103 % Contribution of Dominant Family: 27.18 % ( Chironomidae ) Family Biotic Index: 5.91 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 18.45 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 169 ( Optimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 80 ' / < 1.0 ' Substrate: Cobbles, gravel, silt....StreamBank Vegetation/Stability: Trees, shrubs, grasses/Fair Canopy: Open....Other: Water temp. 19.1 C /pH 7.5 SU /DO 9.1 mg/L /Cond 313 umhos; Suburban periphyton, filamentous algae, minnow, crayfish; storm sewers ------

Station: AN0421 Green Brook, Raymond Rd, Watchung Twp, Somerset/Union County Chatham USGS Quadrangle Date Sampled: 06/08/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Tubificidae 10 40 Stratiomyidae 10 17 Gammaridae 4 15 BloodRed Chironomidae 8 11 Chironomidae 6 8 Elmidae 4 3 Naididae 7 3 Baetidae 4 2 Tetrastemmatidae 7 2 Tipulidae 3 1 Asellidae 8 1 Hydropsychidae 4 1 Planariidae 4 1 Physidae 7 1 Psephenidae 4 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 16 Total Number of Individuals: 108 % Contribution of Dominant Family: 37.04 % ( Tubificidae ) Family Biotic Index: 7.92 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 3.70 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 118 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 38 ' / < 1.0 ' Substrate: Cobbles, gravel/sand....StreamBank Vegetation/Stability: Trees, grass, shrubs/Fair Canopy: Mostly Closed....Other: Water temp. 18.6 C /pH 7.7 SU /DO 7.5 mg/L /Cond 646 umhos; Urban / light industrial (car dealer, shopping mall) flowing storm sewers; trash in stream; minnows, crayfish, filamentous algae ------

Station: AN0422 Stony Brook, West End Ave, North Plainfield Boro, Union County Plainfield USGS Quadrangle Date Sampled: 06/08/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Sphaeriidae 8 20 Gammaridae 4 16 Elmidae 4 13 BloodRed Chironomidae 8 12 Chironomidae 6 8 Tubificidae 10 8 Simuliidae 6 7 Hydropsychidae 4 4 Planariidae 4 4 Physidae 7 3 Baetidae 4 1 Erpobdellidae 8 1 Empididae 6 1 Naididae 7 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 20.00 % ( Sphaeriidae ) Family Biotic Index: 6.23 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 5.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 158 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 28 ' / < 1.0 ' Substrate: Cobbles, gravel....StreamBank Vegetation/Stability: Trees, grass, shrubs/Good Canopy: Partly Open....Other: Water temp. 18.4 C /pH 7.6 SU /DO 7.9 mg/L /Cond 431 umhos; Suburban storm sewer flowing; minnows, darters ------

Station: AN0423 Green Brook, Clinton Avenue, Plainfield, Union/Somerset County Plainfield USGS Quadrangle Date Sampled: 06/08/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 22 BloodRed Chironomidae 8 21 Hydropsychidae 4 12 Tubificidae 10 12 Simuliidae 6 11 Baetidae 4 8 Gammaridae 4 7 Naididae 7 6 Asellidae 8 2 Elmidae 4 2 Tipulidae 3 1 Physidae 7 1 Sphaeriidae 8 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 106 % Contribution of Dominant Family: 20.75 % ( Chironomidae ) Family Biotic Index: 6.40 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 18.87 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 122 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 30' / <0.5' - 1.0' Substrate: Cobbles, Gravel, Silt....StreamBank Vegetation/Stability: Trees, Shrubs/Poor Canopy: Mostly Open....Other: Water temp. 19.6 C / pH 7.6 SU / DO 8.3 mg/L / Cond. 516 umhos; Suburban storm sewers present; soft bottom containing clay ------

Station: AN0424 Bound Brook, Bound Brook Road (Route 28), Middlesex Boro, Middlesex County Plainfield USGS Quadrangle Date Sampled: 06/08/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 46 Tubificidae 10 17 Hydropsychidae 4 13 Chironomidae 6 6 Naididae 7 5 Sphaeriidae 8 4 Corbiculidae 8 3 Elmidae 4 2 Planorbidae 6 2 Physidae 7 1 BloodRed Chironomidae 8 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 101 % Contribution of Dominant Family: 45.54 % ( Gammaridae ) Family Biotic Index: 5.69 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 12.87 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 134 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 47.5' / <1' - 1.0' Substrate: Cobbles, Gravel, Silt....StreamBank Vegetation/Stability: Trees, Grass, Shrubs/Poor Canopy: Mostly Open....Other: Water temp. 21.5 C / pH 7.5 SU / DO 6.8 mg/ L / Cond. 526 umhos; Suburban minnows present; ------

Station: AN0424B Bound Brook, Woodbrook Road, So. Plainfield Boro, Middlesex County Plainfield USGS Quadrangle Date Sampled: 06/17/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Asellidae 8 27 Gammaridae 4 24 Tubificidae 10 19 Sphaeriidae 8 11 Physidae 7 8 Planorbidae 6 4 Chironomidae 6 3 Erpobdellidae 8 3 Lumbriculidae 10 3 Glossiphoniidae 8 1 Planariidae 4 1 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 104 % Contribution of Dominant Family: 25.96 % ( Asellidae ) Family Biotic Index: 7.25 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 82 ( Marginal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Slow....Width/Depth (ft): 16' / 3' Substrate: Mud....StreamBank Vegetation/Stability: Trees, Shrubs/Fair Canopy: Partly Open....Other: Water temp. 21.3 C / pH 7.3 SU / DO 4.6 mg/L / Cond. 612 umhos; Rural, Forested PCB contaminated, "Superfund Site", Woodbrook Park dump site; bull frog, macrophytes present ------

Station: AN0425 Ambrose Brook, Raritan Avenue (Route 514 Spur), Middlesex Boro, Middlesex County Bound Brook USGS Quadrangle Date Sampled: 06/08/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 71 Hydropsychidae 4 8 Physidae 7 5 Tubificidae 10 3 Sphaeriidae 8 3 Chironomidae 6 2 BloodRed Chironomidae 8 2 Tipulidae 3 2 Asellidae 8 1 Lymnaeidae 6 1 Elmidae 4 1 Valvatidae 4 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 100 % Contribution of Dominant Family: 71.00 % ( Gammaridae ) Family Biotic Index: 4.61 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 8.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 168 ( Optimal ) USEPA Protocol Deficiency(s) noted: Gammaridae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 42' / 1.0' Substrate: Cobbles, Gravel/Sand, Snags....StreamBank Vegetation/Stability: Shrubs, Trees, Grass/Good Canopy: Partly Open....Other: Water temp. 21.8 C / pH 7.6 SU / DO 6.0 mg/L / Cond. 475 umhos; Suburban storm sewers, sewage pump, small ditch culvert; minnows ------

Station: AN0425A Ambrose Brook, Behmer Road, Piscataway Twp, Middlesex County Plainfield USGS Quadrangle Date Sampled: 06/17/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 39 Gammaridae 4 32 Elmidae 4 6 Planorbidae 6 5 Physidae 7 5 Tubificidae 10 4 Asellidae 8 2 Ancylidae 6 2 Empididae 6 2 Hydropsychidae 4 1 Coenagrionidae 9 1 Sphaeriidae 8 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 100 % Contribution of Dominant Family: 39.00 % ( Chironomidae ) Family Biotic Index: 5.52 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 1.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 100 ( Marginal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 28' / 1' Substrate: Gravel/sand, Silt....StreamBank Vegetation/Stability: Trees, Shrubs/Poor Canopy: Mostly Closed....Other: Water temp. 23.6 C / pH 7.1 SU / DO 5.3 mg/L / Cond. 453 umhos; Rural / Forested fish, car parts in stream; pipe with flowing water; Trucking company adjacent to stream, dumpsters on left bank ------

Station: AN0426 Green Brook, Lincoln Blvd., Bound Brook Boro, Middlesex/Somerset County Bound Brook USGS Quadrangle Date Sampled: 07/08/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------BloodRed Chironomidae 8 63 Tubificidae 10 22 Gammaridae 4 8 Chironomidae 6 7 Corbiculidae 8 1 ------Statistical Analysis ------Number of Taxa: 5 Total Number of Individuals: 101 % Contribution of Dominant Family: 62.38 % ( BloodRed Chironomidae ) Family Biotic Index: 7.98 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 3 Biological Condition: Severely Impaired Habitat Analysis: 105 ( Marginal ) USEPA Protocol Deficiency(s) noted: BloodRed Chironomidae Family Overwhelmingly Dominant - - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Slow....Width/Depth (ft): 73' / 3' Substrate: Sand, Mud, Silt....StreamBank Vegetation/Stability: Shrubs, Grass, Trees/Fair Canopy: Open....Other: Water temp. 23.0 C / pH 7.3 SU / DO 6.9 mg/L / Cond. 406 umhos; Urban fish present; new bridge constructed in 2002; ------

Station: AN0427 Unt To Raritan River, Route 527 (Main Street), So. Bound Brook Boro, Somerset County Bound Brook USGS Quadrangle Date Sampled: 06/17/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Elmidae 4 50 Chironomidae 6 11 Empididae 6 8 BloodRed Chironomidae 8 8 Hydropsychidae 4 7 Gammaridae 4 5 Corbiculidae 8 4 Asellidae 8 3 Sphaeriidae 8 3 Tubificidae 10 2 Naididae 7 2 Planariidae 4 1 Erpobdellidae 8 1 Planorbidae 6 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 107 % Contribution of Dominant Family: 46.73 % ( Elmidae ) Family Biotic Index: 5.28 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 6.54 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 134 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 21' / <1' Substrate: Gravel/sand, Silt....StreamBank Vegetation/Stability: Trees, Weeds, Vines/Poor Canopy: Mostly Closed....Other: Water temp. 27.2 C / pH 7.5 SU / DO 6.5 mg/L / Cond. 423 umhos; Suburban Storm sewer - flowing downstream of brook; geese, trash, recyclables present ------

Station: AN0428 Raritan River, Bakelite Park (Fieldville Dam), Edison Twp, Somerset/Middlesex County Bound Brook USGS Quadrangle Date Sampled: 07/08/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 29 Chironomidae 6 18 Elmidae 4 11 BloodRed Chironomidae 8 10 Corixidae 9 8 Hydrobiidae 8 7 Hydropsychidae 4 3 Glossosomatidae 0 3 Sphaeriidae 8 3 Planariidae 4 2 Tubificidae 10 2 Potamanthidae 4 1 Asellidae 8 1 Ancylidae 6 1 Viviparidae 6 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 29.00 % ( Gammaridae ) Family Biotic Index: 5.64 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 7.00 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 135 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 150' / 3.0' Substrate: Cobble, Gravel/Sand, Mud....StreamBank Vegetation/Stability: Trees, Grass, Shrubs/Fair Canopy: Open....Other: Water Temp. 27.8 C / pH 7.4 SU/ DO 5.9 mg/L / Cond. 338 umhos; Urban Station downstream of Fieldville Dam; ------

Station: AN0429 Mile Run, Franklin Blvd, New Brunswick, Middlesex County Plainfield USGS Quadrangle Date Sampled: 06/17/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Naididae 7 64 Hydropsychidae 4 15 BloodRed Chironomidae 8 10 Asellidae 8 4 Chironomidae 6 4 Physidae 7 3 Tubificidae 10 2 Erpobdellidae 8 1 Empididae 6 1 Nematoda 6 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 106 % Contribution of Dominant Family: 60.38 % ( Naididae ) Family Biotic Index: 6.72 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 14.15 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 126 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Naididae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 25' / < 1' Substrate: Cobbles, Gravel/Sand....StreamBank Vegetation/Stability: Trees/Fair Canopy: Closed....Other: Water temp. 23.1 C / pH 7.6 SU / DO 7.9 mg/L / Cond. 493 umhos; Urban / Suburban fish; trash in stream; adj to Franklin Twp water pumping station ------

Station: AN0430 Lawrence Brook, Ridge Rd, South Brunswick Twp, Middlesex County Monmouth Junction USGS Quadrangle Date Sampled: 08/05/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Asellidae 8 33 Tubificidae 10 24 Sphaeriidae 8 24 Chironomidae 6 9 BloodRed Chironomidae 8 9 Lumbriculidae 8 3 Naididae 7 2 Glossiphoniidae 8 2 Physidae 7 1 ------Statistical Analysis ------Number of Taxa: 9 Total Number of Individuals: 107 % Contribution of Dominant Family: 30.84 % ( Asellidae ) Family Biotic Index: 8.25 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 156 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 6 ' < 1.0 ' Substrate: Gravel/sand, silt....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Partly Open....Other: Water temp. 21.6 C /pH 6.8 SU /DO 3.5 mg/L Cond 132 umhos; Suburban macrophytes, filamentous algae, fish; ------

Station: AN0431 Lawrence Brook, Davidson Mill Rd., South Brunswick Twp. , Middlesex County New Brunswick USGS Quadrangle Date Sampled: 07/29/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Asellidae 8 43 Chironomidae 6 13 Caenidae 7 10 Sphaeriidae 8 6 BloodRed Chironomidae 8 5 Coenagrionidae 9 5 Hydrobiidae 8 4 Viviparidae 6 3 Gammaridae 4 2 Gomphidae 1 2 Planorbidae 6 2 Heptageniidae 4 2 Glossiphoniidae 8 1 Leptoceridae 4 1 Physidae 7 1 ------Statistical Analysis ------Number of Taxa: 15 Total Number of Individuals: 100 % Contribution of Dominant Family: 43.00 % ( Asellidae ) Family Biotic Index: 7.24 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 13.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 115 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution ------Observations ------Streamwater: Turbid....Flow: Slow....Width/Depth (ft): 30.0' / 1 - 1.5' Substrate: Mud, Silt....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Good Canopy: Partly Open....Other: Water temp. 21.8 C / pH 7.3 SU / DO NA / Cond. 125 umhos; Suburban storm Sewers present; frogs, macrophytes, crayfish present ------

Station: AN0432 Oakeys Brook, Davidson Mill Rd., South Brunswick Twp., Middlesex County New Brunswick USGS Quadrangle Date Sampled: 07/29/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Elmidae 4 41 Planorbidae 6 10 Physidae 7 9 Sphaeriidae 8 6 Chironomidae 6 6 Tipulidae 3 6 Hydrobiidae 8 4 Hydropsychidae 4 4 BloodRed Chironomidae 8 3 Baetidae 4 2 Asellidae 8 2 Planariidae 4 2 Psephenidae 4 2 Philopotamidae 3 1 Dryopidae 5 1 Veliidae 9 1 Leptoceridae 4 1 Naididae 7 1 Tetrastemmatidae 7 1 Heptageniidae 4 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 104 % Contribution of Dominant Family: 39.42 % ( Elmidae ) Family Biotic Index: 5.19 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 5 % EPT: 8.65 NJIS Rating: 18 Biological Condition: Moderately Impaired Habitat Analysis: 167 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 15.0' / 1.0' - 1.5' Substrate: Cobbles, Gravel....StreamBank Vegetation/Stability: Trees, Shrubs/Good Canopy: Mostly Closed....Other: Water Temp. 20.5 C / pH 7.3 SU / DO NA / Cond. 301 umhos; Suburban Storm sewers present.; Frogs and salamanders present. ------

Station: AN0433 Ireland Brook, Riva Rd, North Brunswick Twp, Middlesex County North Brunswick USGS Quadrangle Date Sampled: 07/29/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Elmidae 4 34 Sphaeriidae 8 27 Hydropsychidae 4 7 BloodRed Chironomidae 8 7 Chironomidae 6 7 Gammaridae 4 4 Asellidae 8 2 Enchytraeidae 10 2 Plagiostomidae 4 2 Naididae 7 2 Planorbidae 6 2 Glossiphoniidae 8 1 Ceratopogonidae 6 1 Aeshnidae 3 1 Lumbriculidae 8 1 Cambaridae 5 1 Haliplidae 5 1 Phryganeidae 4 1 Physidae 7 1 Veliidae 9 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 21 Total Number of Individuals: 106 % Contribution of Dominant Family: 32.08 % ( Elmidae ) Family Biotic Index: 5.87 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 7.55 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 183 ( Optimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear, brownish-grey color....Flow: Moderate....Width/Depth (ft): 20 ' / 1.0 - 1.5 ' Substrate: Cobble, gravel, bedrock....StreamBank Vegetation/Stability: Trees, shrubs/Good Canopy: Mostly Closed....Other: Water temp. 22.3 C / pH 7.2 SU / DO N/A / Cond. 147 umhos; Suburban Trout stocked stream; frogs, salamanders, fish, crayfish, macrophytes ------

Station: AN0434 Lawrence Brook, Riva Rd., Milltown Twp. , Middlesex County New Brunswick USGS Quadrangle Date Sampled: 07/29/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 41 Planariidae 4 9 Chironomidae 6 8 BloodRed Chironomidae 8 8 Tubificidae 10 7 Valvatidae 4 6 Asellidae 8 5 Hydropsychidae 4 4 Planorbidae 6 3 Ceratopogonidae 6 2 Coenagrionidae 9 2 Tetrastemmatidae 7 2 Sphaeriidae 8 2 Tipulidae 3 1 Hydrobiidae 8 1 Hydroptilidae 4 1 Libellulidae 9 1 Corduliidae 5 1 Haliplidae 5 1 Physidae 7 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 106 % Contribution of Dominant Family: 38.68 % ( Gammaridae ) Family Biotic Index: 5.48 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 4.72 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 149 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 50' / 2.0' - 3.0' Substrate: Gravel/ Sand, Silt....StreamBank Vegetation/Stability: Shrubs, Trees/Fair Canopy: Mostly Open....Other: Water temp. 26.8 C / pH 7.1 SU / DO N/A / Cond. 200 umhos; Suburban; adjacent to office complex storm sewers present; banks silty; macrophytes, geese present ------

Station: AN0435 Sawmill Brook, Ryders Lane, Milltown Boro, Middlesex County New Brunswick USGS Quadrangle Date Sampled: 07/29/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Tubificidae 10 48 Naididae 7 12 Planorbidae 6 10 BloodRed Chironomidae 8 9 Coenagrionidae 9 6 Chironomidae 6 5 Sphaeriidae 8 4 Physidae 7 2 Planariidae 4 1 Enchytraeidae 10 1 Ancylidae 6 1 Glossiphoniidae 8 1 Haliplidae 5 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 101 % Contribution of Dominant Family: 47.52 % ( Tubificidae ) Family Biotic Index: 8.50 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 76 ( Marginal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Slow....Width/Depth (ft): 20' / <1.0' Substrate: Silt....StreamBank Vegetation/Stability: Grasses, Shrubs, Trees/Poor Canopy: Mostly Closed....Other: Water Temp. 23.5 C / pH 7.0 SU / DO NA / Cond. 430 umhos; Urban Storm sewers present.; Purple loostrife and painted turtles present. ------

Station: AN0436 Mill Brook, Woodbridge Ave Off Nj Tpk., Edison Twp, Middlesex County Plainfield USGS Quadrangle Date Sampled: 07/08/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 41 Chironomidae 6 22 Baetidae 4 15 Lumbricidae 10 7 BloodRed Chironomidae 8 4 Tubificidae 10 3 Tipulidae 3 3 Enchytraeidae 10 2 Naididae 7 2 Tetrastemmatidae 7 2 Gammaridae 4 1 Lumbriculidae 8 1 Leptoceridae 4 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 104 % Contribution of Dominant Family: 39.42 % ( Hydropsychidae ) Family Biotic Index: 5.39 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 54.81 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 155 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 23.5' / <1'-1.5' Substrate: Cobbles, Gravel, Snags....StreamBank Vegetation/Stability: Trees, Shrubs/Fair Canopy: Mostly Closed....Other: Water temp. 22.7 C / pH 7.5 SU / DO 7.3 mg/L / Cond. 682 umhos; Urban Pumping station; oily sheen, sewage smell,; two storm sewers flowing; water cloudy below pipes ------

Station: AN0437 Manalapan Brook, Route 524, Millstone Twp, Monmouth County Roosevelt USGS Quadrangle Date Sampled: 06/15/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 36 Sphaeriidae 8 25 Brachycentridae 1 10 Polycentropodidae 6 8 Elmidae 4 6 Leuctridae 0 4 Tipulidae 3 3 Tubificidae 10 2 Corixidae 9 2 BloodRed Chironomidae 8 2 Aeshnidae 3 1 Curculionidae 7 1 Hydropsychidae 4 1 Lepidostomatidae 1 1 Lumbriculidae 8 1 Leptoceridae 4 1 ------Statistical Analysis ------Number of Taxa: 16 Total Number of Individuals: 104 % Contribution of Dominant Family: 34.62 % ( Chironomidae ) Family Biotic Index: 5.65 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 6 % EPT: 24.04 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 158 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 7.0' / <1' - 1.0' Substrate: Gravel/Sand, Mud....StreamBank Vegetation/Stability: Shrubs, Trees, Grass/Good Canopy: Mostly Open....Other: Water temp. 17.7 C / pH 7.2 SU / DO 8.7 mg/L / Cond. 267 umhos; Rural, Forested frogs present; ------

Station: AN0438 Manalapan Brook, Route 33, Manalapan Twp, Monmouth County Freehold USGS Quadrangle Date Sampled: 06/15/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 41 Hydropsychidae 4 23 Elmidae 4 20 Heptageniidae 4 10 BloodRed Chironomidae 8 3 Tetrastemmatidae 7 2 Sphaeriidae 8 1 ------Statistical Analysis ------Number of Taxa: 7 Total Number of Individuals: 100 % Contribution of Dominant Family: 41.00 % ( Chironomidae ) Family Biotic Index: 5.04 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 33.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 143 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 13.0' / <1' - 2.0' Substrate: Gravel/Sand, Silt....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Fair Canopy: Mostly Closed....Other: Water temp. 22.4 C / pH 7.3 SU / DO 7.3 mg/L / Cond. 235 umhos; Agriculture - cropland, Rural storm sewers present; farm adjacent to stream on left bank; fish present ------

Station: AN0439 Manalapan Brook, Federal Rd, Monroe Twp, Middlesex County Jamesburg USGS Quadrangle Date Sampled: 08/05/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydropsychidae 4 51 Chironomidae 6 20 Heptageniidae 4 14 Tipulidae 3 9 Elmidae 4 3 Corydalidae 0 3 Tubificidae 10 1 Psychomyiidae 2 1 Leptoceridae 4 1 BloodRed Chironomidae 8 1 Simuliidae 6 1 Sphaeriidae 8 1 Naididae 7 1 ------Statistical Analysis ------Number of Taxa: 13 Total Number of Individuals: 107 % Contribution of Dominant Family: 47.66 % ( Hydropsychidae ) Family Biotic Index: 4.34 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 62.62 NJIS Rating: 24 Biological Condition: Nonimpaired Habitat Analysis: 110 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 17 ' / 1 ' Substrate: Mud, clay....StreamBank Vegetation/Stability: Trees, shrubs, grasses/Poor Canopy: Partly Open....Other: Water temp. 21.7 C /pH 6.9 SU /DO 7.7 mg/L /Cond 226 umhos; Agriculture-cropland, rural drainage ditch, trash in stream; ------

Station: AN0440 Manalapan Brook, Old Forge Rd, Helmetta Boro, Middlesex County Jamesburg USGS Quadrangle Date Sampled: 08/05/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 57 Tubificidae 10 20 Sphaeriidae 8 5 Heptageniidae 4 4 Tabanidae 6 3 Brachycentridae 1 2 Calopterygidae 5 2 BloodRed Chironomidae 8 2 Hydropsychidae 4 2 Leptoceridae 4 2 Plagiostomidae 4 1 Sialidae 4 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 101 % Contribution of Dominant Family: 56.44 % ( Chironomidae ) Family Biotic Index: 6.61 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 4 % EPT: 9.90 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 138 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Moderate....Width/Depth (ft): 33 ' / 1 ' Substrate: Sand, mud, silt....StreamBank Vegetation/Stability: Trees, shrubs/Poor Canopy: Mostly Closed....Other: Water temp. 23.0 C /pH 7.0 SU /DO 6.6 mg/L /Cond 191 umhos; Surburban storm sewers, macrophytes, fish; ------

Station: AN0441 Weamaconk Creek, Route 9, Freehold Twp. , Monmouth County Freehold USGS Quadrangle Date Sampled: 08/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Gammaridae 4 50 Chironomidae 6 19 BloodRed Chironomidae 8 9 Tubificidae 10 5 Veliidae 9 4 Tipulidae 3 3 Calopterygidae 5 2 Lumbriculidae 8 2 Physidae 7 2 Sphaeriidae 8 2 Aeshnidae 3 1 Curculionidae 7 1 Naididae 7 1 Tetrastemmatidae 7 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 102 % Contribution of Dominant Family: 49.02 % ( Gammaridae ) Family Biotic Index: 5.50 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 134 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 8.0' / <1' - 1.0' Substrate: Sand, Silt....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Fair Canopy: Mostly Closed....Other: Water Temp. 21.0 C / pH 7.1 SU / DO 7.0 mg/L / Cond. 280 umhos; Urban Storm sewers present; Frogs present, garbage abundant ------

Station: AN0442 Wemrock Brook, Wemrock Road, Freehold Twp., Monmouth County Freehold USGS Quadrangle Date Sampled: 06/15/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Tubificidae 10 34 Sphaeriidae 8 15 Chironomidae 6 14 Gammaridae 4 11 BloodRed Chironomidae 8 10 Physidae 7 4 Elmidae 4 3 Corydalidae 0 2 Hydropsychidae 4 2 Gyrinidae 3 1 Enchytraeidae 10 1 Hydrophilidae 5 1 Naididae 7 1 Lumbriculidae 10 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 100 % Contribution of Dominant Family: 34.00 % ( Tubificidae ) Family Biotic Index: 7.51 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 2.00 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 148 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid....Flow: Slow....Width/Depth (ft): 6' / 3' Substrate: Mud, Snags....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Good Canopy: Partly Open....Other: Water temp. 23.5 C / pH 7.0 SU / DO 4.4 mg/L / Cond. 374 umhos; Agriculture - cropland (orchards), Rural, Forested barely flowing under bridge; access to stream hazardous; dug out area connected to stream flooded for pump ------

Station: AN0443 Weamaconk Creek, Water St., Englishtown Boro, Monmouth County Freehold USGS Quadrangle Date Sampled: 08/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 23 Gammaridae 4 16 Tubificidae 10 16 BloodRed Chironomidae 8 13 Elmidae 4 8 Naididae 7 8 Corduliidae 5 3 Sphaeriidae 8 2 Corixidae 9 2 Caenidae 7 1 Planariidae 4 1 Coenagrionidae 9 1 Veliidae 9 1 Leptoceridae 4 1 Physidae 7 1 Glossiphoniidae 8 1 Sialidae 4 1 Libellulidae 9 1 ------Statistical Analysis ------Number of Taxa: 18 Total Number of Individuals: 100 % Contribution of Dominant Family: 23.00 % ( Chironomidae ) Family Biotic Index: 6.64 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 2.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 141 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Slow....Width/Depth (ft): 15 ' / 1 - 2 ' Substrate: Mud....StreamBank Vegetation/Stability: Trees, shrubs, grass/Poor Canopy: Partly Open....Other: Water temp. 23.1 C /pH 7.1 SU /DO 6.3 mg/L /Cond 266 umhos; Suburban storm sewers, filamentous algae; ------

Station: AN0444 McGellairds Brook, Route 9, Freehold Twp., Monmouth County Freehold USGS Quadrangle Date Sampled: 08/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Tubificidae 10 37 Chironomidae 6 18 Hydropsychidae 4 11 Elmidae 4 11 Gammaridae 4 8 Sphaeriidae 8 7 Hydrobiidae 8 3 Viviparidae 6 3 BloodRed Chironomidae 8 3 Planariidae 4 2 Calopterygidae 5 2 Leptoceridae 4 2 Gerridae 8 2 Caenidae 7 1 ------Statistical Analysis ------Number of Taxa: 14 Total Number of Individuals: 110 % Contribution of Dominant Family: 33.64 % ( Tubificidae ) Family Biotic Index: 6.99 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 3 % EPT: 12.73 NJIS Rating: 21 Biological Condition: Moderately Impaired Habitat Analysis: 134 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 10.0' / 1.0' Substrate: Gravel/Sand, Silt....StreamBank Vegetation/Stability: Shrubs, trees, vines/Fair Canopy: Mostly closed....Other: Water temp. 27.7 C / pH 7.1 SU / DO 4.5 mg/L / Cond. 205 umhos; Urban; storm sewers present fish, frogs, crayfish observed; trash in stream ------

Station: AN0445 Tepehemus Brook, Tennent Rd., Manalapan Twp., Monmouth County Freehold USGS Quadrangle Date Sampled: 08/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 64 BloodRed Chironomidae 8 15 Elmidae 4 9 Tubificidae 10 6 Calopterygidae 5 3 Cambaridae 5 3 Aeshnidae 3 2 Hydropsychidae 4 2 Enchytraeidae 10 1 Lampyridae 6 1 Veliidae 9 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 108 % Contribution of Dominant Family: 59.26 % ( Chironomidae ) Family Biotic Index: 6.22 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 1.85 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 122 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Moderate....Width/Depth (ft): 13.0' / <1' - 1.5' Substrate: Sand, Mud, Silt....StreamBank Vegetation/Stability: Grasses, Shrubs, Trees/Poor Canopy: Partly Open....Other: Water Temp. 22.2 C / pH 7.0 SU / DO 6.3 mg/L / Cond. 269 umhos; Agriculture-cropland, Suburban Minnows and crayfish present; ------

Station: AN0446 Milford Brook, Pease Rd., Manalapan Twp., Monmouth County Freehold USGS Quadrangle Date Sampled: 08/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Sphaeriidae 8 32 Chironomidae 6 18 BloodRed Chironomidae 8 11 Tubificidae 10 6 Lumbriculidae 8 5 Calopterygidae 5 4 Tipulidae 3 4 Aeshnidae 3 3 Elmidae 4 3 Phryganeidae 4 3 Physidae 7 2 Pleuroceridae 6 2 Veliidae 9 2 Corydalidae 0 1 Empididae 6 1 Hydropsychidae 4 1 Cordulegastridae 3 1 Enchytraeidae 10 1 Coenagrionidae 9 1 Tabanidae 6 1 ------Statistical Analysis ------Number of Taxa: 20 Total Number of Individuals: 102 % Contribution of Dominant Family: 31.37 % ( Sphaeriidae ) Family Biotic Index: 6.85 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 3.92 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 127 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Slow....Width/Depth (ft): 11 ' / 1 ' Substrate: Gravel/Sand, Mud, Silt, Concrete, Large Rocks....StreamBank Vegetation/Stability: Trees, Shrubs/Poor Canopy: Mostly Closed....Other: Water Temp. 21.5 C / pH 7.1 SU / DO 7.6 mg/L / Cond. 261 umhos; Suburban, new construction upstream Creosote smell, Fish and Frogs present; ------

Station: AN0447 Mcgellairds Brook, Rt. 527, Englishtown Boro, Monmouth County Freehold USGS Quadrangle Date Sampled: 08/03/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Tubificidae 10 41 Chironomidae 6 13 Calopterygidae 5 10 Hydrobiidae 8 9 Elmidae 4 6 Gammaridae 4 6 Cambaridae 5 6 Sphaeriidae 8 5 Coenagrionidae 9 3 Viviparidae 6 1 Corduliidae 5 1 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 101 % Contribution of Dominant Family: 40.59 % ( Tubificidae ) Family Biotic Index: 7.58 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 127 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear, greyish color....Flow: Moderate....Width/Depth (ft): 10.0' / 2.0' Substrate: Sand, Mud, Silt....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Poor Canopy: Partly Open....Other: Water temp. 22.2 C / pH 7.0 SU / DO 6.9 mg/L / Cond. 253 umhos; Suburban storm sewers; minnows, crayfish present ------

Station: AN0448 Matchaponix Brook, Route 527, Manalapan Twp., Monmouth County Freehold USGS Quadrangle Date Sampled: 08/24/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Hydrobiidae 8 32 Chironomidae 6 20 Gammaridae 4 10 Elmidae 4 8 Calopterygidae 5 5 Tubificidae 10 5 Tetrastemmatidae 7 5 Sphaeriidae 8 5 Hydropsychidae 4 3 Aeshnidae 3 1 BloodRed Chironomidae 8 1 Planariidae 4 1 Gomphidae 1 1 Corydalidae 0 1 Physidae 7 1 Naididae 7 1 ------Statistical Analysis ------Number of Taxa: 16 Total Number of Individuals: 100 % Contribution of Dominant Family: 32.00 % ( Hydrobiidae ) Family Biotic Index: 6.40 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 3.00 NJIS Rating: 15 Biological Condition: Moderately Impaired Habitat Analysis: 133 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Slow....Width/Depth (ft): 23.0' / 2.0' Substrate: Sand, Silt, Snags....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Poor Canopy: Mostly Closed....Other: Water temp. 20.8 C / pH 5.9 SU / DO 8.2 mg/L / Cond. 250 umhos; Suburban; Englistown Auction on right bank fish present; ------

Station: AN0449 Pine Brook, Pension Rd., Manalapan Twp. , Monmouth County Freehold USGS Quadrangle Date Sampled: 08/24/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------BloodRed Chironomidae 8 49 Tubificidae 10 25 Chironomidae 6 15 Coenagrionidae 9 4 Aeshnidae 3 3 Elmidae 4 1 Enchytraeidae 10 1 Libellulidae 9 1 Tipulidae 3 1 ------Statistical Analysis ------Number of Taxa: 9 Total Number of Individuals: 100 % Contribution of Dominant Family: 49.00 % ( BloodRed Chironomidae ) Family Biotic Index: 8.03 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 0 % EPT: 0.00 NJIS Rating: 6 Biological Condition: Severely Impaired Habitat Analysis: 115 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid, greenish-grey color....Flow: Moderate....Width/Depth (ft): 20.0' / 1.0' Substrate: Gravel/Sand, Silt, Snag, Root Mats....StreamBank Vegetation/Stability: Trees/Poor Canopy: Mostly Closed....Other: Water temp. 19.3 C / pH 3.4 SU / DO 7.8 mg/L / Cond. 445 umhos; Suburban storm sewers present; iron precipitate present ------

Station: AN0450 Barclay Brook, Rt. 527, Madison Twp., Middlesex County Freehold USGS Quadrangle Date Sampled: 08/24/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Calopterygidae 5 33 Tubificidae 10 27 Chironomidae 6 22 Tipulidae 3 9 Sialidae 4 8 Polycentropodidae 6 4 Corydalidae 0 2 Phryganeidae 4 1 BloodRed Chironomidae 8 1 ------Statistical Analysis ------Number of Taxa: 9 Total Number of Individuals: 107 % Contribution of Dominant Family: 30.84 % ( Calopterygidae ) Family Biotic Index: 6.19 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 2 % EPT: 4.67 NJIS Rating: 12 Biological Condition: Moderately Impaired Habitat Analysis: 146 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 19.0' / <1.0' Substrate: Gravel, Sand, Mud, Snags....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Fair Canopy: Partly Open....Other: Water Temp. 20.0 C / pH 3.6 SU / DO 8.2 mg/L / Cond. 320 umhos; Agriculture - cropland, Suburban. Fish, frogs, and macrophytes present. ; ------

Station: AN0451 Matchaponix Brook, Texas Rd., Madison Twp. , Middlesex County Freehold USGS Quadrangle Date Sampled: 08/24/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 46 Tubificidae 10 26 BloodRed Chironomidae 8 24 Calopterygidae 5 4 Elmidae 4 2 Gammaridae 4 1 Hydropsychidae 4 1 Coenagrionidae 9 1 Lumbricidae 10 1 Veliidae 9 1 Sphaeriidae 8 1 Naididae 7 1 ------Statistical Analysis ------Number of Taxa: 12 Total Number of Individuals: 109 % Contribution of Dominant Family: 42.20 % ( Chironomidae ) Family Biotic Index: 7.40 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 0.92 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 113 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: - Significant Organic Pollution - Paucity of Clean Water Organisms ------Observations ------Streamwater: Clear....Flow: Moderate....Width/Depth (ft): 48.0' / 1.0' Substrate: Mud, Silt....StreamBank Vegetation/Stability: Trees, Shrubs/Poor Canopy: Mostly Closed....Other: Water Temp. 22.5 C / pH 7.9 SU / DO 8.1 mg/L / Cond. 441 umhos; Suburban, Forested Storm sewers present.; Macrophytes, fish, frogs present. ------

Station: AN0452 Iresick Brook, Route 527, Madison Twp., Middlesex County South Amboy USGS Quadrangle Date Sampled: 09/02/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 52 Sphaeriidae 8 21 Lumbriculidae 8 10 BloodRed Chironomidae 8 6 Tubificidae 10 5 Asellidae 8 2 Phryganeidae 4 2 Dytiscidae 5 1 Naididae 7 1 ------Statistical Analysis ------Number of Taxa: 9 Total Number of Individuals: 100 % Contribution of Dominant Family: 52.00 % ( Chironomidae ) Family Biotic Index: 6.94 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 2.00 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 78 ( Marginal ) USEPA Protocol Deficiency(s) noted: - Paucity of Clean Water Organisms ------Observations ------Streamwater: Turbid/Red....Flow: Slow....Width/Depth (ft): 20.0' / <1.0' Substrate: Gravel/Sand, Mud, Silt, Snags....StreamBank Vegetation/Stability: Grasses, Trees/Poor Canopy: Open....Other: Water Temp. 20.7 C / pH 6.9 SU / DO 6.4 mg/L / Cond. 189 umhos; Suburban. storm sewers, abundant iron floc present; majority of right bank cemented, garbage in stream ------

Station: AN0453 Deep Run, Route 9, Madison Twp., Middlesex County South Amboy USGS Quadrangle Date Sampled: 09/02/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------Chironomidae 6 59 Tubificidae 10 17 BloodRed Chironomidae 8 6 Sialidae 4 4 Enchytraeidae 10 3 Calopterygidae 5 2 Aeshnidae 3 1 Elmidae 4 1 Curculionidae 7 1 Corydalidae 0 1 Phryganeidae 4 1 ------Statistical Analysis ------Number of Taxa: 11 Total Number of Individuals: 96 % Contribution of Dominant Family: 61.46 % ( Chironomidae ) Family Biotic Index: 6.73 E+P+T (Ephemeroptera, Plecoptera, Trichoptera): 1 % EPT: 1.04 NJIS Rating: 9 Biological Condition: Moderately Impaired Habitat Analysis: 122 ( Suboptimal ) USEPA Protocol Deficiency(s) noted: Chironomidae Family Overwhelmingly Dominant - - Paucity of Clean Water Organisms ------Observations ------Streamwater: Slightly Turbid....Flow: Slow....Width/Depth (ft): 32.0' / 4.0' Substrate: Gravel/Sand, Mud, Silt....StreamBank Vegetation/Stability: Trees, Shrubs, Grass/Poor Canopy: Closed....Other: Water Temp. 21.3 C / pH 4.7 SU / DO 7.7 mg/L / Cond. 257 umhos; Urban. Storm sewers and drainage ditch present. ------

Station: AN0454 Deep Run, Rt 516, Madison Twp, Middlesex County South Amboy USGS Quadrangle Date Sampled: 9/2/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------

Not sampled due to bridge construction.

Station: AN0455 Tennent Bk, Old Bridge-South Amboy Rd, Madison Twp, Middlesex County South Amboy USGS Quadrangle Date Sampled: 9/2/04 ------. Family Tolerance Number of Family Value (FTV) Individuals ------

Not Sampled. Site was determined to be tidal and this violates our sampling protocol therefore it will be dropped from our program.