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The New Antitumor Drug ABTL0812 Inhibits the Akt/Mtorc1 Axis By

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The New Antitumor Drug ABTL0812 Inhibits the Akt/Mtorc1 Axis By Published OnlineFirst December 15, 2015; DOI: 10.1158/1078-0432.CCR-15-1808 Cancer Therapy: Preclinical Clinical Cancer Research The New Antitumor Drug ABTL0812 Inhibits the Akt/mTORC1 Axis by Upregulating Tribbles-3 Pseudokinase Tatiana Erazo1, Mar Lorente2,3, Anna Lopez-Plana 4, Pau Munoz-Guardiola~ 1,5, Patricia Fernandez-Nogueira 4, Jose A. García-Martínez5, Paloma Bragado4, Gemma Fuster4, María Salazar2, Jordi Espadaler5, Javier Hernandez-Losa 6, Jose Ramon Bayascas7, Marc Cortal5, Laura Vidal8, Pedro Gascon 4,8, Mariana Gomez-Ferreria 5, Jose Alfon 5, Guillermo Velasco2,3, Carles Domenech 5, and Jose M. Lizcano1 Abstract Purpose: ABTL0812 is a novel first-in-class, small molecule tion of Tribbles-3 pseudokinase (TRIB3) gene expression. Upre- which showed antiproliferative effect on tumor cells in pheno- gulated TRIB3 binds cellular Akt, preventing its activation by typic assays. Here we describe the mechanism of action of this upstream kinases, resulting in Akt inhibition and suppression of antitumor drug, which is currently in clinical development. the Akt/mTORC1 axis. Pharmacologic inhibition of PPARa/g or Experimental Design: We investigated the effect of ABTL0812 TRIB3 silencing prevented ABTL0812-induced cell death. on cancer cell death, proliferation, and modulation of intracel- ABTL0812 treatment induced Akt inhibition in cancer cells, tumor lular signaling pathways, using human lung (A549) and pancre- xenografts, and peripheral blood mononuclear cells from patients atic (MiaPaCa-2) cancer cells and tumor xenografts. To identify enrolled in phase I/Ib first-in-human clinical trial. cellular targets, we performed in silico high-throughput screening Conclusions: ABTL0812 has a unique and novel mechanism of comparing ABTL0812 chemical structure against ChEMBL15 action, that defines a new and drugable cellular route that links database. PPARs to Akt/mTORC1 axis, where TRIB3 pseudokinase plays a Results: ABTL0812 inhibited Akt/mTORC1 axis, resulting in central role. Activation of this route (PPARa/g-TRIB3-Akt- impaired cancer cell proliferation and autophagy-mediated cell mTORC1) leads to autophagy-mediated cancer cell death. Given death. In silico screening led us to identify PPARs, PPARa and the low toxicity and high tolerability of ABTL0812, our results PPARg as the cellular targets of ABTL0812. We showed that support further development of ABTL0812 as a promising anti- ABTL0812 activates both PPAR receptors, resulting in upregula- cancer therapy. Clin Cancer Res; 1–12. Ó2015 AACR. Introduction The PI3K/AKT/mTORC1 (mTOR complex-1) signaling path- 1 Protein Kinases and Signal Transduction Laboratory, Institut de Neu- way drives signals from ligand-stimulated receptor tyrosine rociencies and Departament de Bioquímica i Biologia Molecular, Uni- versitat Autonoma de Barcelona, Bellaterra, Barcelona, Spain. kinases (RTK) to proteins that control cell metabolism, growth, 2Department of Biochemistry and Molecular Biology, Faculty of size, survival, and angiogenesis (1). In response to growth or Biology, Universidad Complutense, Madrid, Spain. 3Instituto de survival factors, phosphorylated RTKs activate PI3K, which gen- Investigacion Sanitaria del Hospital Clínico San Carlos (IdISSC), erates the phosphatidylinositol 3,4,5-trisphosphate (PIP3) sec- Madrid, Spain. 4Area of Molecular and Translational Oncology, IDIBAPS, Fundacio Clínic, Universitat de Barcelona, Barcelona, Spain. ond messenger and allows the recruitment of protein kinase Akt to 5Ability Pharmaceuticals, SL, Edifici Eureka, Campus UAB, Bellaterra, the cellular membrane. Akt binds PIP3 through its PH domain, 6 Barcelona, Catalonia, Spain. Pathology Department, Hospital Uni- resulting in a conformational change that enables its activation versitari Vall d'Hebron, Barcelona, Spain. 7Institut de Neurociencies and Department of Biochemistry and Molecular Biology, Universitat through phosphorylation of two critical residues: Thr308 at the Autonoma de Barcelona, Barcelona, Spain. 8Medical Oncology T-loop of the kinase domain by the phosphoinositide-dependent Department, Novel Therapeutics Unit, Hospital Clínic Barcelona. kinase-1 (PDK1), and Ser473 at the C-terminal hydrophobic Barcelona, Catalonia, Spain. motif by mTOR complex-2 (mTORC2; refs. 2, 3). Active Akt Note: Supplementary data for this article are available at Clinical Cancer phosphorylates a plethora of substrates that regulate cell survival Research Online (http://clincancerres.aacrjournals.org/). and metabolism, and also cell growth through subsequent acti- Corresponding Author: Jose M. Lizcano, Institut de Neurociencies and Departa- vation of mTORC1. Akt phosphorylation of PRAS40 and TSC2 ment de Bioquímica i Biologia Molecular, Facultat de Medicina, Universitat leads to activation of mTORC1, which promotes protein trans- Autonoma de Barcelona, Bellaterra, Barcelona 08193, Spain. Phone: 34 935- lation and synthesis by phosphorylating ribosomal S6-Kinase 813-076; Fax: 34 935-811-573; E-mail: [email protected] (S6K) and the elongation factor 4EBP-1 (4). doi: 10.1158/1078-0432.CCR-15-1808 Human cancer is very often associated with activation of the Ó2015 American Association for Cancer Research. PI3K/Akt/mTORC1 pathway, mainly due to overexpression or www.aacrjournals.org OF1 Downloaded from clincancerres.aacrjournals.org on September 30, 2021. © 2015 American Association for Cancer Research. Published OnlineFirst December 15, 2015; DOI: 10.1158/1078-0432.CCR-15-1808 Erazo et al. plementary Table S1. Akt was immunoprecipitated following the Translational Relevance protocol previously described (11). Hyperactivation of the Akt/mTORC1 axis is commonly observed in many human cancers and blocking this pathway Transmission electron microscopy is an important anticancer strategy. Inhibition of Akt is a valid Cells treated with ABTL0812 or vehicle (ethanol) were pro- target for anticancer therapy and several AKT inhibitors are cessed as described before (12). Pellets were embedded in Epo- under clinical development. We present ABTL0812, a first-in- nate-12TM resin (Ted Pella). Ultrathin (70-nm thick) sections class small molecule with a unique mechanism of action. In were contrasted with uranyl-acetate and lead citrate solutions, and cells and tumor xenografts models, ABTL0812 induces upre- visualized in transmission electron microscope (Jeol JEM-1400) gulation of TRIB3 pseudokinase. TRIB3 binds Akt, preventing equipped with a CCD- GATAN-ES1000W Erlangshen camera. its activation by upstream kinases and resulting in an effective inhibition of the Akt/mTORC1 axis and in autophagy-medi- Xenograft models fi ated cancer cell death. ABTL0812 is currently in phase I/Ib rst- For A549 model, athymic female nude mice (n ¼ 5 per group) in-human clinical trial in patients with advance solid tumors. were injected subcutaneously with 5  106 cells in each flank. Preliminary results show that ABTL0812 also induces Akt When tumors reached 80 to 100 mm3, mice were randomly inhibition in humans. These evidences, together with a very distributed into treatment groups and administered the corre- low toxicity and high tolerability, support further develop- sponding treatments. ABTL0812 was administered by oral gavage ment of ABTL0812. at 120 mg/kg every day. Docetaxel was administered by intraper- itoneal route at 15 mg/kg once a week. MiaPaCa-2 cells were injected in athymic male mice (n ¼ 5 per group) with 10  106 cells in one flank. When tumors reached 80 to 160 mm3, mice activating mutations of RTKs and PI3K, to deletions/mutations of were randomly distributed and treated with vehicle or ABTL0812 the PIP3 phosphatase PTEN and to amplification of Akt (3, 5). 120 mg/kg by oral gavage five times per week. Tumor volumes 2 These types of tumors are hypersensitive to inhibition of each of were measured as (length  width )/2, twice a week. All proce- the components of the PI3K/Akt/mTORC1 axis, and therefore dures involving animal were performed with the approval of the major efforts have been taken to develop inhibitors of this Hospital Clinic Animal Experimentation Committee, according signaling pathway (6). Akt acts as a central node of this pathway, to Spanish official regulations. and hyperactivated Akt is a common feature observed in human solid tumors (3, 5). Therefore, Akt has been proposed as an Pharmacokinetics of ABTL0812 in mice and rats interesting target in cancer therapy. Two classes of Akt inhibitors ABTL0812 was orally (gavage, 100 mg/kg) and intravenously are now in clinical development: ATP-competitive inhibitors (10 mg/kg) administered to male CD-1 mice. ABTL0812 was (such as GSK690693) which target active Akt, and allosteric quantified in plasma by validated LC/MS-MS methods and phar- inhibitors (such as MK-2206 and perifosine, currently in phase macokinetic parameters were calculated with WinNonlin II and phase III clinical trials, respectively) that by binding the PH software. domain of Akt prevent its activation by upstream kinases (3, 7). Here we present ABTL0812, a first-in-class molecule with anti- Clinical trial and isolation of PBMC from patients tumor activity which is currently in phase I/Ib clinical trials in ABTL0812 is currently in clinical evaluation in a phase I/Ib trial patients with advanced solid tumors. We provide evidences show- in patients with advanced solid tumors. This study was approved ing that ABTL0812 inhibits Akt phosphorylation by upregulating by the local Ethics Committee of the Hospital Clinic Barcelona the expression of TRIB3,a pseudokinase that binds and inhibits Akt, and the
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