Physicochemical Changes in Hazelnut, Olive Pomace, Grapeseed and Sunflower Oils
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Food Sci. Technol. Res., 15 (5), 519–524, 2009 Physicochemical Changes in Hazelnut, Olive Pomace, Grapeseed and Sunflower Oils Heated at Frying Temperatures * Leyla TEKIN, Mehmet Seçkin ADAY and Emin YILMAZ Çanakkale Onsekiz Mart University, Faculty of Engineering and Architecture, Department of Food Engineering, Terzioglu Campus, 17020, Çanakkale, Turkey. Received December 4, 2008; Accepted June 2, 2009 In order to compare the thermal performances to evaluate suitability for frying, hazelnut oil (HNO), olive pomace oil (OPO), grapeseed oil (GSO) and sunflower oil (SSO) were heated for 5 h / 5 consecutive days at 175 ± 5 ℃, and sampled at the end of each day. Free acidity, peroxide value, conjugated dienes, total polar materials, viscosity, and CIE color values were measured. Highest values of free acidity, conju- gated dienes, total polar materials and viscosity were observed in grapeseed oil sample. GSO has reached to a very viscous state during the earlier hours of the operation; hence it is not suitable for frying. Better thermal performances have found with HNO and OPO when compared to the control oil, SSO. Therefore, both oils can alternatively be used as new frying oils effectively. Keywords: heating, hazelnut oil, olive pomace oil, sunflower oil, grapeseed oil, performance Introduction 2007). In literature, many different approaches to develop Food frying is a common practice in the world, used both better frying oils and healthier fried products, and analytical in industrial scale and in small facilities as well as homes. methods have been published (Su and White, 2004; Garcia Fried foods are very popular and main items of fast-food and Valdes, 2006; Ghazali et al., 2007). Sometimes, thermal sector. By frying the surface of food yields some crispness performances of frying oils were determined by heating together with some aromatic compounds which consumers around frying temperatures without any actual food frying like most (Blumenthal, 1996). for quick screening (Shimizu et al., 2004). In frying process, the frying oil serves as a heat transfer Turkey provides around 75% of world total hazelnut medium and an ingredient of fried foods. During the process, production. In recent years, refined hazelnut oil (HNO) has heat transfer from oil to food and cooking of food, mean- become important liquid edible oil. The literature for hazel- time water vaporization from food into the oil and then to nut oil in frying is scarce. In a previous study, hazelnut oil the atmosphere, penetration of frying oil into the capillaries is heated at 121℃ in a pressure cooker and quality changes formed in food, air-oil mixing at the surface and similar phe- were monitored. Hazelnut oil was found better in stabil- nomena occur constantly. As a result of these, oil hydrolysis, ity than corn and sunflower oils (Karabulut et al., 2004). In oxidation, polymerization and Maillard reactions and some another study, potatoes were fried in microwave oven with other chemical reactions take place. The products of these hazelnut, sunflower and corn oils, and the highest fat absorp- reactions accumulate and deteriorate oil, and may develop tion was found with hazelnut oil (Şahin et al., 2007). Better toxicity in fried foods (Ghazali et al., 2007). To control fry- frying quality of virgin olive oils is rather a well established ing oil quality, guidelines have been established in many phenomenon (IOOC, 2008), but frying studies dealing with countries. In Turkey, Official Notification of the criteria to refined olive pomace (edible pomace oil) oil (OPO) is also control liquid and solid frying fats/oils rules content of po- limited. In Turkey, olive pomace oil has just become ed- lar compounds as ≤ 25%, and smoke point > 170℃ (TGK, ible oil in 2007 by officially employing the Codex Standard (Codex, 2003). In a study, characters of olive and sunflower *To whom correspondence should be addressed. oils (SSO) heated at frying temperatures were compared, E-mail: [email protected] and sunflower oil were found to be more sensitive to thermal 520 L. TEKIN et al. treatment, especially viscosity of oils increased very quickly the producers. Grapeseed oil (Olitalia, Italy) was purchased (Garcia and Valdes, 2006). In another study, olive pomace from a local market. The fatty acids and main minor compo- oil was used in the search of preparing ideal frying oil mix- nents composition of the oils are provided by the producers ture compositions (Leonardi, 2005). Grapeseed oil (GSO) and shown in Table 1. All chemicals used in the analyses is a very new one for consumers. Grapeseed contains about were purchased from Merck (Darmstad, Germany) and were 7-20% oil. The hexane extracted oil has usually refined be- of analytical grade. All analyzes were duplicate in this study. fore commercial use. The oil is rich in linoleic acid (70%), Thermal treatment Thermal treatments of all frying oils phenolic compounds and tocol group compounds (Matthäus, were accomplished with a 1.5 L Cleo CLF-2500 Fryer (Kara- 2008). The generation of free radical intermediates during the köy, İstanbul) at 175 ± 5℃. First day, the fryer filled up with heating (105-108℃) of grapeseed oil has been established 1.5 L of fresh oils. Then oil sample was treated for 5 con- by a combination of spin trapping and electron paramagnetic secutive days by heating 5 h daily to total a 25 h of thermal resonance spectroscopy (Goodman et al., 1995). The effects treatment time. Sampling of oils was after the end of each of microwave and air-drying of grapeseeds on the physical day by taking 200 mL of oil into amber colored and sealed and chemical parameters of their oils were investigated. Mi- glass. Until and during the analyses oil samples were kept in crowave treatment improved oil yield and increased viscos- fridge. ity, conjugated dienes, and peroxide values while reducing Chemical analyses Total free fatty acids (FFA) of the pigment contents (K-410 and K-670 values) and p-anisidine samples were measured by titrating 1 g sample dissolved in and saponification values (Mazza et al., 1998). There is no 95% ethanol against phenolphthalein indicator according to published study with grapeseed oil in frying. On the other AOCS method Ca 5a-40 (AOCS, 1997a), and results are giv- hand, many studies with sunflower seed oil in frying opera- en as oleic acid (%). Peroxide value (PV) was determined by tions can be found elsewhere. reacting sample dissolved in a mixture of chloroform-acetic Since different oils have very diverse fatty acid and mi- acid (2:3) with a solution of potassium iodide in darkness, nor components composition, it is expected that they would then free iodine titration with a sodium thiosulfate solution behave very differently at higher temperatures. The aim of according to AOCS method Cd 8-53 (AOCS, 1997b). The this study was to compare the physicochemical changes in results were expressed as milliequivalents of active oxygen hazelnut oil (HNO), olive pomace oil (OPO), sunflower oil per kilogram of oil (meq O2 / kg sample). Conjugated diene (SSO) and grapeseed oil (GSO) heated at around 175 ± 5℃ (CD) values of the samples were determined as per AOCS Ti for 25 h in 5 consecutive days. 1a-64 (AOCS, 1997c) method. Oil samples were dissolved in iso-octane and diluted when necessary. The absorbance Materials and Methods was then measured at 233 nm, and %CD was calculated Materials In this study, the commercial refined hazelnut by the given equation. Direct reading of total polar materi- oil (Ordu Oil Co., Ordu, Turkey), refined olive pomace oil als (% TPM) was accomplished by immersing the probe of (Marbil Oil Co., İzmir, Turkey), refined-winterized sunflower Testo 265 sensor (Lenzkirch, Germany) into the oil samples seed oil (Aymar Oil Co., İstanbul, Turkey) were donated by at 45℃ according to the instructions of the instrument. The Table 1. The fatty acids and main minor components composition of the oil samples provided by the producers. HNO OPO SSO GSO Fatty acid composition (%) Myristic (C14:0), max 0.03 < 0.05 0.06 0.05 Palmitic (C16:0) 5.34 7.5-20 5.94 8.56 Palmitoleic (C16:1), max 0.18 0.3-3.5 0.11 0.18 Heptadecenoic (C17:1), max 0.07 < 0.3 0.03 0.04 Stearic (C18:0) 2.61 0.5-5.00 3.77 4.41 Oleic (C18:1) 81.62 55-83 29.81 22.00 Linoleic (C18:2) 9.71 3.5-21 58.78 64.07 Linolenic (C18:3), max 0.09 < 0.9 0.13 0.32 Arachidic (C20:0) 0.12 < 0.6 0.25 0.15 Gadoleic (C20:1), max 0.17 < 0.4 0.16 0.15 Behenic (C22:0) 0.02 < 0.2 0.65 0.02 Total Sterols (mg/kg) 1200-2000 > 1800 2400-4500 5000-6000 Total Tocopherol (mg/kg) 250-500 trace 400-900 100-300 Thermal Stability of Oils 521 instrument can estimate total polar materials in frying fats at frying oil samples at the 0 and 25th hours of thermal treat- 40-210℃ by ±2% reliance. The instrument was calibrated to ment for the chemical and physical measurements, and the measure TPM in 0.5-40% range by ±2% accuracy with the significant differences among the days of heating (0-25 h) for standard oil provided. Above the calibration range, reading each oil per each measurement were determined by one-way can still be obtained but accuracy may change as the user’s analysis of variance using the Duncan’s multiple range com- manual indicated. parison at 95% of confidence. In addition, correlation coef- Physical analyses Refractive index of the frying oil ficients of the measured oil parameters for all samples were samples were measured under daylight with a 2WAJ model determined.