Multiple Choice Identify the Letter of the Choice That Best Completes the Statement Or Answers the Question. 1. the Branch of B
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Aerobic Respiration
Life is based on redox • All energy generation in biological systems is due to redox (reduction-oxidation) reactions Aerobic Respiration: + - C6H12O6 + 6 H2O ==> 6 CO2 + 24 H +24 e oxidation electron donor (aka energy source) + - (O2+ 4H + 4e ==> 2H2O) x6 reduction electron acceptor --------------------------------------- C6H12O6 + 6 O2 ==> 6 CO2 + 6 H2O overall reaction (24 electrons) Types of bacterial metabolisms • While eukaryotes only reduce O2 and oxidize organic compounds, prokaryotes can use a variety of electron donors and acceptors, organic and inorganic. - • Aerobic respiration: e acceptor is O2 - • Anaerobic respiration: e acceptor is not O2 • Fermentation: e- donor and acceptor are organic molecules • Chemolithotrophy: e- donor and acceptor are inorganic molecules • Phototrophy: e- donor is light and e- acceptor is either organic or inorganic all microorganisms energy source? chemical light chemotroph phototroph carbon source? carbon source? organic organic CO CO compound 2 compound 2 chemoheterotroph chemoautotroph photoheterotroph photoautotroph e- acceptor? Nitrifying and sulfur- use H O to reduce CO ? oxidizing bacteria 2 2 green non-sulfur and O Other than O 2 2 purple non-sulfur bacteria anoxygenic oxygenic photosynthesis: photosynthesis: green sulfur and most bacteria Organic Inorganic cyanobacteria compound compound purple sulfur bacteria fermentative organism anaerobic respiration: nitrate, sulfate, Fe(III) Aerobic or anaerobic respiration Chemolithotrophy Important molecules Redox Electron Carrier: for example the -
IB HL Biology: Ecology Review Fall 2017 Populations 1. Define the Following Terms Associated with Population Ecology; Population and Carrying Capacity
IB HL Biology: Ecology Review Fall 2017 Populations 1. Define the following terms associated with population ecology; population and carrying capacity. 2. What processes contribute to changes in population size? 3. What are some factors which can increase the carrying capacity of a population? Decrease? 4. What is quadrat sampling? When would it be used? Communities 5. Define the following terms; community, autotroph, heterotroph, producer, primary consumer, secondary consumer, detritivore and saprotroph. 6. What is the initial energy source for all communities? 7. Be able to read food webs and determine the trophic level of different species. 8. Choose 2 regions below and determine the Simpson’s Diversity Index value for these regions. Which region is more diverse? A. An area of the Black Forest in Germany contains 134 pitch pines, 24 douglas firs, and 53 red pines. B. A meadow contains 1532 chestnut oaks, 342 black cherry trees, 12 white ash trees, and 1022 yellow birches. C. You school science classroom contains 12 beetles, 34 termites, 84 ants, 93 fleas, and 1 butterfly. D. An African park contains 15 lions, 94 giraffes, 1000 wildebeests, 50 elephants, and 5 hyenas. Choose more areas if you need more practice. 9. What is a keystone species? 10. Distinguish between primary and secondary succession. Ecosystems 11. What is an ecosystem? 12. Explain the 10% rule of energy transfer. How is the energy lost between trophic levels? 13. Review the Carbon Cycle. What are the main sources of carbon dioxide on earth? 14. Review the Nitrogen Cycle. 15. Distinguish between Gross Primary Productivity and Net Primary Productivity. -
Chapter 11 – PROKARYOTES: Survey of the Bacteria & Archaea
Chapter 11 – PROKARYOTES: Survey of the Bacteria & Archaea 1. The Bacteria 2. The Archaea Important Metabolic Terms Oxygen tolerance/usage: aerobic – requires or can use oxygen (O2) anaerobic – does not require or cannot tolerate O2 Energy usage: autotroph – uses CO2 as a carbon source • photoautotroph – uses light as an energy source • chemoautotroph – gets energy from inorganic mol. heterotroph – requires an organic carbon source • chemoheterotroph – gets energy & carbon from organic molecules …more Important Terms Facultative vs Obligate: facultative – “able to, but not requiring” e.g. • facultative anaerobes – can survive w/ or w/o O2 obligate – “absolutely requires” e.g. • obligate anaerobes – cannot tolerate O2 • obligate intracellular parasite – can only survive within a host cell The 2 Prokaryotic Domains Overview of the Bacterial Domain We will look at examples from several bacterial phyla grouped largely based on rRNA (ribotyping): Gram+ bacteria • Firmicutes (low G+C), Actinobacteria (high G+C) Proteobacteria (Gram- heterotrophs mainly) Gram- nonproteobacteria (photoautotrophs) Chlamydiae (no peptidoglycan in cell walls) Spirochaetes (coiled due to axial filaments) Bacteroides (mostly anaerobic) 1. The Gram+ Bacteria Gram+ Bacteria The Gram+ bacteria are found in 2 different phyla: Firmicutes • low G+C content (usually less than 50%) • many common pathogens Actinobacteria • high G+C content (greater than 50%) • characterized by branching filaments Firmicutes Characteristics associated with this phylum: • low G+C Gram+ bacteria -
Principles of Ecology Section ●2 Flow of Energy in an Ecosystem
chapter 2 Principles of Ecology section ●2 Flow of Energy in an Ecosystem -!). )DEA Before You Read Autotrophs capture energy, making it available for all If a pet had to survive without your care, how would its diet members of a food web. change? Write your ideas on the lines below. Read about how What You’ll Learn organisms get food and energy in their environment. ■ the fl ow of energy through an ecosystem ■ food chains, food webs, and pyramid models 3TUDY#OACH Read to Learn Make Flash Cards Make a fl ash card for each question Energy in an Ecosystem heading in this section. On the One way to study the interactions within an ecosystem is to back of the fl ash card, write the trace how energy fl ows through the system. All organisms are answer to the question. Use the classifi ed by the way they obtain energy. fl ash cards to review what you have learned. How do autotrophs obtain energy? All green plants and other organisms that produce their own food are the primary producers of food in an ecosystem. They are called autotrophs. An autotroph (AW tuh trohf) is an organism that captures energy from sunlight or inorganic substances to produce food. Autotrophs make energy available for all other organisms in the ecosystem. How do heterotrophs differ from autotrophs? A heterotroph (HE tuh roh trohf), also called a consumer, is an organism that obtains energy by consuming other organisms. A heterotroph that consumes only plants is an herbivore (HUR buh vor). Cows, rabbits, and grasshoppers are herbivores. -
Determining the Contribution of Aquatic Autotrophs to the Carbon Budgets of Restored Wetlands in the Sacramento-San Joaquin River Delta
Determining the Contribution of Aquatic Autotrophs to the Carbon Budgets of Restored Wetlands in the Sacramento-San Joaquin River Delta A thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Environmental Sciences Ian J. UTZ College of Natural Resources, University of California, Berkeley August 6, 2016 Contents 1 Introduction 8 2 Background 11 2.1 Ecological Engineering . 12 2.2 Successional Community Ecology . 13 2.3 Food Web Interactions . 14 2.4 Plant Physiology . 15 2.5 Physical Habitat . 16 3 Methods 18 3.1 Locations . 18 3.2 Measurements . 22 3.2.1 Remotely sensing changes in aquatic primary produc- tivity using high resolution satellite imagery. 22 3.2.2 Remotely sensing changes in aquatic primary produc- tivity using continuous recording of changes in dis- solved oxygen and carbon dioxide. 23 3.2.3 Measuring physical habitat covariates to characterize environmental baselines. 24 3.2.4 Conducting field experiments and biomass estimation to attribute changes in dissolved oxygen to differences in the presence and intensity of aquatic primary pro- duction. 26 3.3 Treatments . 28 4 Results 32 4.1 Physical covariate measurements . 32 1 4.2 Remotely sensing changes in aquatic primary productivity us- ing high resolution satellite imagery. 34 4.3 Remotely sensing changes in aquatic primary productivity us- ing continuous recording of changes in dissolved oxygen, dis- solved carbon dioxide, and phenocam greenness data. 37 4.4 Conducting field experiments and biomass sampling to at- tribute changes in dissolved oxygen to differences in the pres- ence and intensity of aquatic primary production. -
Bioenergetics Module a Anchor 3
Bioenergetics Module A Anchor 3 Key Concepts: - ATP can easily release and store energy by breaking and re-forming the bonds between its phosphate groups. This characteristic of ATP makes it exceptionally useful as a basic energy source for all cells. - In the process of photosynthesis, plants convert the energy of sunlight into chemical energy stored in the bonds of carbohydrates. - Photosynthetic organisms capture energy from sunlight with pigments. - An electron carrier is a compound that can accept a pair of high-energy electrons and transfer them, along with most of their energy, to another molecule. - Photosynthesis uses the energy of sunlight to convert water and carbon dioxide into high- energy sugars and oxygen. - Among the most important factors that affect photosynthesis are temperature, light intensity, and the availability of water. - Organisms get the energy they need from food. - Cellular respiration is the process that releases energy from food in the presence of oxygen. - Photosynthesis removes carbon dioxide from the atmosphere and cellular respiration puts it back. Photosynthesis releases oxygen into the atmosphere, and cellular respiration uses that oxygen to release energy from food. - In the absence of oxygen, fermentation releases energy from food molecules by producing ATP. - For short, quick bursts of energy, the body uses ATP already in muscles as well as ATP made by lactic acid fermentation. - For exercise longer than about 90 seconds, cellular respiration is the only way to continue generating a supply of ATP. Vocabulary: ATP ADP autotroph heterotroph Photosynthesis pigment chlorophyll chloroplast Thylakoid stroma NADP+/NADPH calorie Cellular respiration aerobic anaerobic fermentation Glucose ATP and Energy Molecules: 1. -
7.014 Lectures 16 &17: the Biosphere & Carbon and Energy Metabolism
MIT Department of Biology 7.014 Introductory Biology, Spring 2005 7.014 Lectures 16 &17: The Biosphere & Carbon and Energy Metabolism Simplified Summary of Microbial Metabolism The metabolism of different types of organisms drives the biogeochemical cycles of the biosphere. Balanced oxidation and reduction reactions keep the system from “running down”. All living organisms can be ordered into two groups1, autotrophs and heterotrophs, according to what they use as their carbon source. Within these groups the metabolism of organisms can be further classified according to their source of energy and electrons. Autotrophs: Those organisms get their energy from light (photoautotrophs) or reduced inorganic compounds (chemoautotrophs), and their carbon from CO2 through one of the following processes: Photosynthesis (aerobic) — Light energy used to reduce CO2 to organic carbon using H2O as a source of electrons. O2 evolved from splitting H2O. (Plants, algae, cyanobacteria) Bacterial Photosynthesis (anaerobic) — Light energy used to reduce CO2 to organic carbon (same as photosynthesis). H2S is used as the electron donor instead of H2O. (e.g. purple sulfur bacteria) Chemosynthesis (aerobic) — Energy from the oxidation of inorganic molecules is used to reduce CO2 to organic carbon (bacteria only). -2 e.g. sulfur oxidizing bacteria H2S → S → SO4 + - • nitrifying bacteria NH4 → NO2 → NO3 iron oxidizing bacteria Fe+2 → Fe+3 methane oxidizing bacteria (methanotrophs) CH4 → CO2 Heterotrophs: These organisms get their energy and carbon from organic compounds (supplied by autotrophs through the food web) through one or more of the following processes: Aerobic Respiration (aerobic) ⎯ Oxidation of organic compounds to CO2 and H2O, yielding energy for biological work. -
An Evolutionary Optimization of a Rhodopsin-Based Phototrophic
Kim et al. Microb Cell Fact (2017) 16:111 DOI 10.1186/s12934-017-0725-6 Microbial Cell Factories RESEARCH Open Access An evolutionary optimization of a rhodopsin‑based phototrophic metabolism in Escherichia coli Hyun Aaron Kim1,7†, Hyun Ju Kim2†, Jihoon Park3, Ah Reum Choi4, Kyoo Heo6, Haeyoung Jeong5, Kwang‑Hwan Jung4, Yeong‑Jae Seok6, Pil Kim3* and Sang Jun Lee2* Abstract Background: The expression of the Gloeobacter rhodopsin (GR) in a chemotrophic Escherichia coli enables the light- driven phototrophic energy generation. Adaptive laboratory evolution has been used for acquiring desired pheno‑ type of microbial cells and for the elucidation of basic mechanism of molecular evolution. To develop an optimized strain for the artifcially acquired phototrophic metabolism, an ancestral E. coli expressing GR was adaptively evolved in a chemostat reactor with constant illumination and limited glucose conditions. This study was emphasized at an unexpected genomic mutation contributed to the improvement of microbial performance. Results: During the chemostat culture, increase of cell size was observed, which were distinguished from that of the typical rod-shaped ancestral cells. A descendant ET5 strain was randomly isolated from the chemostat culture at 88-days. The phototrophic growth and the light-induced proton pumping of the ET5 strain were twofold and eightfold greater, respectively, than those of the ancestral E. coli strain. Single point mutation of C1082A at dgcQ gene (encoding diguanylate cyclase, also known as the yedQ gene) in the chromosome of ET5 strain was identifed from whole genome sequencing analysis. An ancestral E. coli complemented with the same dgcQ mutation from the ET5 was repeated the subsequently enhancements of light-driven phototrophic growth and proton pumping. -
Food Web Invasion
8 EXPLORE { LESSON } Food Web Invasion Students develop a food web with ten native species and show the impact of two invasive species. OBJECTIVES List at least 10 connections Explain the impacts of Diagram a food web that between Great Lakes coastal changes on a Great Lakes coastal shows the interconnectedness of organisms in a food web habitat food web native species and two invasive species SUBJECT VOCABULARY MATERIALS TIME/DURATION autotroph Great Lakes food chain and Ecology decomposer food web images 90 minutes + food chain paper presentations food web pencils PREREQUISITE heterotroph drawing supplies SETTING #7: The Great Race invasive species Indoors for Survival primary producer Outdoors primary consumer quaternary consumer secondary consumer tertiary consumer This Great Lakes in My World 9-12 activity is aligned to the Common Core State Standards (as available). This alignment is available on your Great Lakes in My World 9-12 USB flash drive in the “Standards” folder and on-line at http://www.greatlakes.org/GLiMWstandards. background plants and animals that inhabit coastal habitats along the Great Lakes. There are many ways to model a food web. It INVASIVE SPECIES IN THE Great LAKES REGION is important that the information on organisms is accurate. Invasive species travel, often accidentally, from their native Students may be creative with this project – it might be a ecosystem to a new ecosystem. Waterborne commerce two-or three-dimensional model. It may take the shape of moves millions of tons of cargo annually through the Great a puzzle, a web, a mural, a graphic computer-design, or Lakes. -
The Term Autotroph Refers to an Organism That
The Term Autotroph Refers To An Organism That Cob is perfectively cerated after passionate Lucas demount his superhets perspectively. Orlando usually proverb perplexedly or keeks especially when transhuman Hagan predestinates secularly and joylessly. Erik never revaccinates any vampirism floodlighting hand-to-hand, is Vito bearable and investigative enough? An listener other that to form organic matter, these are dependent because autotrophs are often the presence of the adult and! Growth Terminology The two ways that microbial organisms can be classified are as autotrophs supply you own energy or as heterotrophs use the products. Renewal of that the term organism to an autotroph refers to the host. The terms autotroph and hetetotroph refer to C-source What team the. Autotrophic you are an exponential growth. The terms refer, an ecological roles to know the aphid colony, or from these structures except during their. Plants are always prime symbol of autotrophs using photosynthesis All other organisms must obtain use red food that comes from other organisms in the probably of fats. Green algae are another hint of organisms that often produce their own time via photosynthesis. Just beneath the reverse, this layer hence the forest is composed of small trees and shrubs. Inorganic materials as independent and by coupling process in or chemolithotrophs use sunlight into the term refers to refer to the same as energy from the highest tide. Click on autotrophs that an organism that meiosis was a source is termed a broad term refers to refer to talk about given volume, and attempt to. We believe you with reference to refer? Burton's Microbiology for standing Health Sciences. -
MICROBIAL METABOLISM: AUTOTROPHS Overview of Autotrophy • Imagine Being Hungry, Walking Outside, Taking Off Your Shirt, Lying
MICROBIAL METABOLISM: AUTOTROPHS Overview of Autotrophy • Imagine being hungry, walking outside, taking off your shirt, lying in the sun for a few hours, becoming totally full (fat even!), and being done eating. No stores, no lines, no choices, just sunlight --- and the machinery of an autotroph --- and some CO2 and a couple of other requirements (water --- and H2S or Hydrogen gas, if you happen to be an anerobe) • Autotroph = gets all carbon from CO2, organic C not required (for C-source). Use special metabolic cycle: Calvin-Benson cycle • Refers to C-source only; some organisms still require organic C as energy source Calvin-Benson cycle • Each CO2 is added to a 5-C acceptor molecule (ribulose 1,5 bis-phosphate) • Immediately split into two 3-C molecules (3-phosphoglyceric acid) • Must add phosphate group (from ATP) and hydrogen (from NADPH) to get reduced product, 3 - phospho-glyceraldehyde (PGA) • Cannot take all (PGA) as product --- must regenerate some more acceptor to keep cycle going. How? • Take 5 PGA molecules (5 x 3C = 15 C atoms). Rearrange through series of reactions to make 3 5 - C molecules (still 15 C atoms). Add ATP to each, make 3 acceptor molecules (ribulose 1,5 bis-phosphate) • Net result: To get 1 PGA (3-C) as reduced product, need 3 CO2 molecules, added to 3 acceptor molecules ----> six 3 - C molecules, use 6 ATP and 6 NADPH ----> 6 PGA molecules; five of these are used to regenerate acceptor molecules (+ 3ATP), one PGA can leave cycle and be used by cell. Summary • Actual cyce exports 3-C reduced molecules: look at balanced equation: 3 CO2 + 9 ATP + 6 NADPH -----> 3-phospho-glyceraldehyde (PGA) + 9 ADP + 9 NADP+ • Often want to look at balanced equation relative to 6C synthesis. -
Culturing Microorganisms
Unit 2 Lecture 4 Unit 2 Lecture 4 Culturing Microorganisms Methods of Culturing and Examining Microorganisms - TheSix I’s. Inoculation is the placement of a small portion of the specimen onto media. Once the plate is inoculated it is streaked to isolate the bacteria by spreading out the inoculum. Isolation is the processes to obtain individual colonies (pour plate, streak plate, and loop dilution are all isolation methods). The plate is placed into a cabinet that is set a specified temperature for a period of time (usually one day). This is known as incubation. The culture is observed or inspected for growth. One then compiles all the data in a process called information gathering. Finally, based upon the results of biochemical and other tests an identification of the organism is made. A pure culture (axenic) results if only a single known species grows. Mixed cultures result if more than one known species grows. And a contaminated culture results if a previously pure or mixed culture has an unwanted organism inoculated onto it. Why is the cultivation of microbes important? It is important to identify microbes that cause disease in order that appropriate therapy is given to the patient. Types of Media Physical State Chemical Composition Functional Type . General Purpose . Enriched . Liquid . Selective . Semisolid . Synthetic . Differential . Solid (can be (chemically . Anaerobic converted to defined) Growth liquid) . Nonsynthetic . Specimen . Solid (cannot (not chemically transport be converted defined) . Assay to liquid) . Enumeration Media can be differentiated by a number of different aspects. One can describe the media’s physical state. Liquid media do not solidify at temperatures above freezing.