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Aerobic Respiration
Life is based on redox • All energy generation in biological systems is due to redox (reduction-oxidation) reactions Aerobic Respiration: + - C6H12O6 + 6 H2O ==> 6 CO2 + 24 H +24 e oxidation electron donor (aka energy source) + - (O2+ 4H + 4e ==> 2H2O) x6 reduction electron acceptor --------------------------------------- C6H12O6 + 6 O2 ==> 6 CO2 + 6 H2O overall reaction (24 electrons) Types of bacterial metabolisms • While eukaryotes only reduce O2 and oxidize organic compounds, prokaryotes can use a variety of electron donors and acceptors, organic and inorganic. - • Aerobic respiration: e acceptor is O2 - • Anaerobic respiration: e acceptor is not O2 • Fermentation: e- donor and acceptor are organic molecules • Chemolithotrophy: e- donor and acceptor are inorganic molecules • Phototrophy: e- donor is light and e- acceptor is either organic or inorganic all microorganisms energy source? chemical light chemotroph phototroph carbon source? carbon source? organic organic CO CO compound 2 compound 2 chemoheterotroph chemoautotroph photoheterotroph photoautotroph e- acceptor? Nitrifying and sulfur- use H O to reduce CO ? oxidizing bacteria 2 2 green non-sulfur and O Other than O 2 2 purple non-sulfur bacteria anoxygenic oxygenic photosynthesis: photosynthesis: green sulfur and most bacteria Organic Inorganic cyanobacteria compound compound purple sulfur bacteria fermentative organism anaerobic respiration: nitrate, sulfate, Fe(III) Aerobic or anaerobic respiration Chemolithotrophy Important molecules Redox Electron Carrier: for example the -
Hattori Hachi.’ My Favourite Books
Praise for ‘A great debut novel.’ The Sun ‘Hattie is joined on her terrifying adventures by some fantastic characters, you can’t help but want to be one of them by the end – or maybe you’re brave enough to want to be Hattie herself . .’ Chicklish ‘Hachi is strong, independent, clever and remarkable in every way . I can’t shout loud enough about Hattori Hachi.’ My Favourite Books ‘Jane Prowse has completely nailed this novel. I loved the descriptions, the action, the heart-stopping moments where deceit lurks just around the corner. The story is fabulous, while almost hidden profoundness is scattered in every chapter.’ Flamingnet reviewer, age 12 ‘Hattori Hachi is like the female Jackie Chan, she has all the ninjutsu skills and all the moves! The Revenge of Praying Mantis is one of my all time favourite books! I love the fact that both boys and girls can enjoy it.’ Jessica, age 12 ‘I couldn’t put this book down – it was absolutely brilliant!’ Hugo, age 9 ‘This delightful book is full of ninja action and packed with clever surprises that will hook anyone who reads it!’ Hollymay, age 15 ‘This was the best book I’ve ever read. It was exciting and thrilling and when I started reading it, I could not put it back down.’ Roshane, age 18 ‘Amazing! Couldn’t put it down. Bought from my school after the author’s talk and finished it on the very next day! Jack, age 12 This edition published by Silver Fox Productions Ltd, 2012 www.silverfoxproductions.co.uk First published in Great Britain in 2009 by Piccadilly Press Ltd. -
Five Kingdoms
University of Central Florida STARS Electronic Theses and Dissertations, 2004-2019 2008 Five Kingdoms Kelle Groom University of Central Florida Part of the Creative Writing Commons Find similar works at: https://stars.library.ucf.edu/etd University of Central Florida Libraries http://library.ucf.edu This Masters Thesis (Open Access) is brought to you for free and open access by STARS. It has been accepted for inclusion in Electronic Theses and Dissertations, 2004-2019 by an authorized administrator of STARS. For more information, please contact [email protected]. STARS Citation Groom, Kelle, "Five Kingdoms" (2008). Electronic Theses and Dissertations, 2004-2019. 3519. https://stars.library.ucf.edu/etd/3519 FIVE KINGDOMS by KELLE GROOM M.A. University of Central Florida, 1995 B.A. University of Central Florida, 1989 A thesis submitted in partial fulfillment of the requirements for the degree of Master of Fine Arts in Creative Writing/Poetry in the Department of English in the College of Arts and Humanities at the University of Central Florida Orlando, Florida Fall Term 2008 Major Professor: Don Stap © 2008 Kelle Groom ii ABSTRACT GROOM, KELLE . Five Kingdoms. (Under the direction of Don Stap.) Five Kingdoms is a collection of 55 poems in three sections. The title refers to the five kingdoms of life, encompassing every living thing. Section I explores political themes and addresses subjects that reach across a broad expanse of time—from the oldest bones of a child and the oldest map of the world to the bombing of Fallujah in the current Iraq war. Connections between physical and metaphysical worlds are examined. -
Tiësto Mp3 Mp3, Flac, Wma
Tiësto Mp3 mp3, flac, wma DOWNLOAD LINKS (Clickable) Genre: Electronic Album: Mp3 Country: Ukraine Released: 2009 Style: House, Tech House, Trance MP3 version RAR size: 1790 mb FLAC version RAR size: 1648 mb WMA version RAR size: 1111 mb Rating: 4.2 Votes: 925 Other Formats: AC3 APE AU XM MP3 AA MP1 Tracklist Hide Credits 1 Ten Seconds Before Sunrise 2 Everything 3 Do You Feel Me 4 Carpe Noctum 5 Driving To Heaven 6 Sweet Things 7 Bright Morningstar 8 Break My Fall 9 In The Dark 10 Dance4Life 11 Elements Of Life 12 He's A Pirate 13 La Hacienda 14 Contact Don't Belong 15 Featuring – Leah Vice (Sydenham Dub) 16 Remix – Sydenham* 17 Madras Tell Me 18 Featuring – Jessica* Summerfish (Scandall Sunset On Ibiza Mix) 19 Remix – Scandall 20 Searching For Truth 21 The Sun'll Shine (Sunrise Mix) 22 See The Difference Inside (Inside Mix) Somewhere Inside Of Me 23 Featuring – Julie Thompson 24 High Glow Lonely 25 Featuring – Alanah Hide And Seek (Tiesto's In Search Of Sunrise Remix) 26 Remix – Tiesto* 27 A New Dawn 28 What You Need (NC's In Love With Prog Mix) Trozitos De Navidad (Primavera Remix) 29 Remix – Primavera 30 Don't Speak 31 Arguru Falling 32 Featuring – Anita Kelsey Fall To Pieces 33 Featuring – Jennifer Rene Imagination (Tiesto Remix) 34 Remix – Tiesto* 35 Mercury Room 36 Chase My Rabbit 37 Reflect 38 Different Day, Different Light 39 Dancing Water 40 Breathing 41 Feel The Sun Rise 42 Wasted Yohkoh (King Unique Original Mix) 43 Remix – King Unique Space Katzle (Jerome Sydenham Remix) 44 Remix – Jerome Sydenham Feel The Rhythm (Ton TB Dub -
DJ Central Playlist
www.djcentral.tv DJ Central Playlist Aired 30th November 2012 Artist Label Clip / Song Name (or Interview) Tiesto Feat. Edward Carnby Ultra Records Alone In The Dark Jes Ultra Records Imagination AAA Blue Pie Records La Boum Fatale Tiesto Ultra Records 643 (Love's On Fire) Tiesto Ultra Records Traffic Tiesto Feat. BT Ultra Records Love Comes Again DJ Diamond Blue Pie Records Brazil 2011 Tiesto Feat. Kirsty Hawkshaw Ultra Records Just Be Tiesto Feat. Kirsty Hawkshaw Ultra Records Urban Train Tiesto Feat. Kirsty Hawkshaw Ultra Records Just Be The Subs Lektrolov Don't Stop Tiesto Ultra Records Adagio For Strings Tiesto Feat. Maxi Jazz Ultra Records Dance4Life Tiesto Feat. Christian Burns Ultra Records In The Dark Tiesto Feat. BT Ultra Records Break My Fall Cosmic Gate Ultra Records Fire Wire Tiesto Ultra Records Elements of Life Jes Ultra Records Love Song Cosmic Gate Ultra Records Barra Jes Ultra Records Closer Richard Vission Vs Luciana Blue Pie Records When It Feels This Good Episode 9 – 30th & 2nd December 2012 Channel 183: The Aurora Foxtel Network Fridays 9:30 PM AEST - Australia If you want to get your videos played on the show, all you have to do is email our music partner Blue Pie Records on [email protected] and send us a link to your clip on youtube or Vimeo and the team will get back to you within 72 hours and let you know if the clip has made the show quality standards. Thank you for supporting DJ Central! Copyright © DJ Central Hong Kong LTD 2013. -
Roxbox by Artist (Hed) Planet Earth 2 Play Feat
RoxBox by Artist (Hed) Planet Earth 2 Play Feat. Thomas Jules & Bartender Jucxi D Blackout Careless Whisper Other Side 2 Unlimited 10 Years No Limit Actions & Motives 20 Fingers Beautiful Short Dick Man Drug Of Choice 21 Demands Fix Me Give Me A Minute Fix Me (Acoustic) 2Pac Shoot It Out Changes Through The Iris Dear Mama Wasteland How Do You Want It 10,000 Maniacs Until The End Of Time Because The Night 2Pac Feat Dr. Dre Candy Everybody Wants California Love Like The Weather 2Pac Feat. Dr Dre More Than This California Love These Are The Days 2Pac Feat. Elton John Trouble Me Ghetto Gospel 101 Dalmations 2Pac Feat. Eminem Cruella De Vil One Day At A Time 10cc 2Pac Feat. Notorious B.I.G. Dreadlock Holiday Runnin' Good Morning Judge 3 Doors Down I'm Not In Love Away From The Sun The Things We Do For Love Be Like That Things We Do For Love Behind Those Eyes 112 Citizen Soldier Dance With Me Duck & Run Peaches & Cream Every Time You Go Right Here For You Here By Me U Already Know Here Without You 112 Feat. Ludacris It's Not My Time (I Won't Go) Hot & Wet Kryptonite 112 Feat. Super Cat Landing In London Na Na Na Let Me Be Myself 12 Gauge Let Me Go Dunkie Butt Live For Today 12 Stones Loser Arms Of A Stranger Road I'm On Far Away When I'm Gone Shadows When You're Young We Are One 3 Of A Kind 1910 Fruitgum Co. -
DJ Central Playlist
www.djcentral.tv DJ Central Playlist Aired 30th December 2012 Artist Label Clip / Song Name (or Interview) Tiesto Feat. Edward Carnby Ultra Music Alone In The Dark JES Ultra Records Imagination AAA Blue Pie Records La Boum Fatale Tiesto Ultra Music 643(Love's On Fire ALVARO Blue Pie Records Make The Crowd Go Tiesto Feat. BT Ultra Records Love Comes Again DJ Diamond Blue Pie Records Brazil 2011 Tiesto Feat. Kirsty Hawkshaw Ultra Records Just Be Tiesto Feat. Kirsty Hawkshaw Ultra Records Urban Train Tiesto Feat. Maxi Jazz Ultra Records Dance4Life Tiesto Feat. Christian Burns Ultra Records In The Dark Tiesto Feat. BT Ultra Records Break My Fall Cosmic Gate Ultra Records Fire Wire Blame Xelon Entertainment On My Own Star Fuckers Blue Pie Records One Night Alvaro Blue Pie Records Make The Crowd Go Meital De Razon & Asi Tai Blue Pie Records Le Lo Le Cosmic Gate Blue Pie Records Flying Blind Richard Vission Vs Luciana Blue Pie Records When It Feels This Good Episode 13 – 30th December 2012 Channel 183: The Aurora Foxtel Network Fridays 9:30 PM AEST - Australia If you want to get your videos played on the show, all you have to do is email our music partner Blue Pie Records on [email protected] and send us a link to your clip on youtube or Vimeo and the team will get back to you within 72 hours and let you know if the clip has made the show quality standards. Thank you for supporting DJ Central! Copyright © DJ Central Hong Kong LTD 2013. -
7.014 Lectures 16 &17: the Biosphere & Carbon and Energy Metabolism
MIT Department of Biology 7.014 Introductory Biology, Spring 2005 7.014 Lectures 16 &17: The Biosphere & Carbon and Energy Metabolism Simplified Summary of Microbial Metabolism The metabolism of different types of organisms drives the biogeochemical cycles of the biosphere. Balanced oxidation and reduction reactions keep the system from “running down”. All living organisms can be ordered into two groups1, autotrophs and heterotrophs, according to what they use as their carbon source. Within these groups the metabolism of organisms can be further classified according to their source of energy and electrons. Autotrophs: Those organisms get their energy from light (photoautotrophs) or reduced inorganic compounds (chemoautotrophs), and their carbon from CO2 through one of the following processes: Photosynthesis (aerobic) — Light energy used to reduce CO2 to organic carbon using H2O as a source of electrons. O2 evolved from splitting H2O. (Plants, algae, cyanobacteria) Bacterial Photosynthesis (anaerobic) — Light energy used to reduce CO2 to organic carbon (same as photosynthesis). H2S is used as the electron donor instead of H2O. (e.g. purple sulfur bacteria) Chemosynthesis (aerobic) — Energy from the oxidation of inorganic molecules is used to reduce CO2 to organic carbon (bacteria only). -2 e.g. sulfur oxidizing bacteria H2S → S → SO4 + - • nitrifying bacteria NH4 → NO2 → NO3 iron oxidizing bacteria Fe+2 → Fe+3 methane oxidizing bacteria (methanotrophs) CH4 → CO2 Heterotrophs: These organisms get their energy and carbon from organic compounds (supplied by autotrophs through the food web) through one or more of the following processes: Aerobic Respiration (aerobic) ⎯ Oxidation of organic compounds to CO2 and H2O, yielding energy for biological work. -
An Evolutionary Optimization of a Rhodopsin-Based Phototrophic
Kim et al. Microb Cell Fact (2017) 16:111 DOI 10.1186/s12934-017-0725-6 Microbial Cell Factories RESEARCH Open Access An evolutionary optimization of a rhodopsin‑based phototrophic metabolism in Escherichia coli Hyun Aaron Kim1,7†, Hyun Ju Kim2†, Jihoon Park3, Ah Reum Choi4, Kyoo Heo6, Haeyoung Jeong5, Kwang‑Hwan Jung4, Yeong‑Jae Seok6, Pil Kim3* and Sang Jun Lee2* Abstract Background: The expression of the Gloeobacter rhodopsin (GR) in a chemotrophic Escherichia coli enables the light- driven phototrophic energy generation. Adaptive laboratory evolution has been used for acquiring desired pheno‑ type of microbial cells and for the elucidation of basic mechanism of molecular evolution. To develop an optimized strain for the artifcially acquired phototrophic metabolism, an ancestral E. coli expressing GR was adaptively evolved in a chemostat reactor with constant illumination and limited glucose conditions. This study was emphasized at an unexpected genomic mutation contributed to the improvement of microbial performance. Results: During the chemostat culture, increase of cell size was observed, which were distinguished from that of the typical rod-shaped ancestral cells. A descendant ET5 strain was randomly isolated from the chemostat culture at 88-days. The phototrophic growth and the light-induced proton pumping of the ET5 strain were twofold and eightfold greater, respectively, than those of the ancestral E. coli strain. Single point mutation of C1082A at dgcQ gene (encoding diguanylate cyclase, also known as the yedQ gene) in the chromosome of ET5 strain was identifed from whole genome sequencing analysis. An ancestral E. coli complemented with the same dgcQ mutation from the ET5 was repeated the subsequently enhancements of light-driven phototrophic growth and proton pumping. -
Leksykon Polskiej I Światowej Muzyki Elektronicznej
Piotr Mulawka Leksykon polskiej i światowej muzyki elektronicznej „Zrealizowano w ramach programu stypendialnego Ministra Kultury i Dziedzictwa Narodowego-Kultura w sieci” Wydawca: Piotr Mulawka [email protected] © 2020 Wszelkie prawa zastrzeżone ISBN 978-83-943331-4-0 2 Przedmowa Muzyka elektroniczna narodziła się w latach 50-tych XX wieku, a do jej powstania przyczyniły się zdobycze techniki z końca XIX wieku m.in. telefon- pierwsze urządzenie służące do przesyłania dźwięków na odległość (Aleksander Graham Bell), fonograf- pierwsze urządzenie zapisujące dźwięk (Thomas Alv Edison 1877), gramofon (Emile Berliner 1887). Jak podają źródła, w 1948 roku francuski badacz, kompozytor, akustyk Pierre Schaeffer (1910-1995) nagrał za pomocą mikrofonu dźwięki naturalne m.in. (śpiew ptaków, hałas uliczny, rozmowy) i próbował je przekształcać. Tak powstała muzyka nazwana konkretną (fr. musigue concrete). W tym samym roku wyemitował w radiu „Koncert szumów”. Jego najważniejszą kompozycją okazał się utwór pt. „Symphonie pour un homme seul” z 1950 roku. W kolejnych latach muzykę konkretną łączono z muzyką tradycyjną. Oto pionierzy tego eksperymentu: John Cage i Yannis Xenakis. Muzyka konkretna pojawiła się w kompozycji Rogera Watersa. Utwór ten trafił na ścieżkę dźwiękową do filmu „The Body” (1970). Grupa Beaver and Krause wprowadziła muzykę konkretną do utworu „Walking Green Algae Blues” z albumu „In A Wild Sanctuary” (1970), a zespół Pink Floyd w „Animals” (1977). Pierwsze próby tworzenia muzyki elektronicznej miały miejsce w Darmstadt (w Niemczech) na Międzynarodowych Kursach Nowej Muzyki w 1950 roku. W 1951 roku powstało pierwsze studio muzyki elektronicznej przy Rozgłośni Radia Zachodnioniemieckiego w Kolonii (NWDR- Nordwestdeutscher Rundfunk). Tu tworzyli: H. Eimert (Glockenspiel 1953), K. Stockhausen (Elektronische Studie I, II-1951-1954), H. -
Culturing Microorganisms
Unit 2 Lecture 4 Unit 2 Lecture 4 Culturing Microorganisms Methods of Culturing and Examining Microorganisms - TheSix I’s. Inoculation is the placement of a small portion of the specimen onto media. Once the plate is inoculated it is streaked to isolate the bacteria by spreading out the inoculum. Isolation is the processes to obtain individual colonies (pour plate, streak plate, and loop dilution are all isolation methods). The plate is placed into a cabinet that is set a specified temperature for a period of time (usually one day). This is known as incubation. The culture is observed or inspected for growth. One then compiles all the data in a process called information gathering. Finally, based upon the results of biochemical and other tests an identification of the organism is made. A pure culture (axenic) results if only a single known species grows. Mixed cultures result if more than one known species grows. And a contaminated culture results if a previously pure or mixed culture has an unwanted organism inoculated onto it. Why is the cultivation of microbes important? It is important to identify microbes that cause disease in order that appropriate therapy is given to the patient. Types of Media Physical State Chemical Composition Functional Type . General Purpose . Enriched . Liquid . Selective . Semisolid . Synthetic . Differential . Solid (can be (chemically . Anaerobic converted to defined) Growth liquid) . Nonsynthetic . Specimen . Solid (cannot (not chemically transport be converted defined) . Assay to liquid) . Enumeration Media can be differentiated by a number of different aspects. One can describe the media’s physical state. Liquid media do not solidify at temperatures above freezing. -
Extremophile Cards
hloroexus aurantiacus “Color-changer, green to orange” einococcus radiodurans yrococcus furiosus Domain: Bacteria “Terrible berry, survives radiation” “Raging reball” Habitat: Hot springs around the world, including Domain: Bacteria Domain: Archaea Yellowstone National Park. Habitat: Widespread, including deserts, hot springs, Habitat: Hydrothermal vents in ocean oor; rst Energy Source: Mixotroph (phototroph and high mountains, polar regions and animal gut. discovered near volcanic islands in Italy. chemotroph). Mainly uses light energy from the sun. In the dark, can use chemical energy from inorganic Energy Source: Chemotroph. Uses chemical energy Energy Source: Chemotroph. Uses chemical energy compounds (sulphur and hydrogen). from organic compounds. from organic compounds. Challenges to Life: Heat, UV exposure, changing Challenges to Life: Dehydration, cold, acidic pH, Challenges to Life: Heat, pressure, changes in levels of light and oxygen radiation, oxidative damage oxygen levels when vent uids contact sea water Adaptation: Can rebuild its genome, after radiation Adaptation: A protein called reverse gyrase helps breaks it into hundreds of fragments, using a unique maintain DNA structure in extreme heat. High Adaptation: Can do both photosynthesis (like DNA-repair mechanism. Produces antioxidants that temperatures tend to unzip double-stranded DNA. plants) and respiration (like animals), allowing it to protect proteins from radiation damage. Reverse gyrase twists DNA so much it can’t unravel. survive a range of light and oxygen levels. It is green during photosynthesis and orange during respiration. Talent: Radioresistant. Survives short doses of Talent: Hyperthermophile. rives in extremely hot radiation up to 5,000 Gy. Grows under constant temperatures. Grows best at 100°C (212°F), Talent: ermophile.