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Figs1 Speciestree Seqcap Phrynomantis microps Microhylidae Breviceps adspersus Brevicipitidae Hemisus marmoratus Hemisotidae Leptopelis rufus Cardioglossa gracilis Arthtroleptis poecilinotus Leptodactylodon ovatus Arthroleptidae Nyctibates corrugatus Trichobatrachus robustus Scotobleps gabonicus Acanthixalus spinosus Acanthixalus sonjae Semnodactylus wealli Paracassina obscura Paracassina kounhiensis Kassininae Phlyctimantis maculata Phlyctimantis verrucosus Phlyctimantis boulengeri Phlyctimantis leonardi Kassina kuvangensis Kassina fusca Kassina lamottei Kassina senegalensis Kassina decorata Kassina maculosa Kassina arboricola Hyperoliidae Kassina cochranae Opisthothylax immaculatus Afrixalus enseticola Tachycnemis seychellensis Heterixalus luteostriatus Malagasy-Seychelles Heterixalus alboguttatus Species Afrixalus vibekensis Afrixalus weidholzi Afrixalus lacustris Afrixalus dorsimaculatus 1 Afrixalus dorsimaculatus 2 Afrixalus knysae Afrixalus spinifrons Hyperoliinae Afrixalus delicatus Afrixalus sylvaticus Afrixalus brachycnemis Afrixalus laevis Afrixalus lacteus Afrixalus fornasini Afrixalus wittei Afrixalus osorioi Afrixalus paradorsalis paradorsalis 2 Afrixalus paradorsalis paradorsalis 1 Afrixalus paradorsalis manengubensis Afrixalus nigeriensis Afrixalus vittiger 1 Afrixalus vittiger 2 Afrixalus dorsalis 3 Afrixalus dorsalis 1 Afrixalus quadrivittatus 1 Afrixalus quadrivittatus 2 Afrixalus dorsalis 2 Afrixalus fulvovittatus 2 Afrixalus fulvovittatus 1 Morerella cyanophthalma Cryptothylax greshoffii Hyperolius semidiscus Hyperolius parkeri Hyperolius pusillus Hyperolius adspersus Hyperolius pseudoargus Hyperolius fusciventris ssp. Hyperolius fusciventris burtoni Hyperolius bolifambae Hyperolius ocellatus ocellatus Hyperolius ocellatus purpurescens Clade 1 Hyperolius sp. nov. ‘Kupe’ Hyperolius camerunensis Hyperolius riggenbachi Hyperolius guttulatus 2 Hyperolius guttulatus 1 Hyperolius phantasticus Hyperolius pardalis Hyperolius steindachneri Hyperolius argus Hyperolius bocagei Hyperolius lamottei Hyperolius tuberculatus Hyperolius dintelmanni Hyperolius parallelus parallelus Hyperolius parallelus marginatus Hyperolius parallelus alborufus Hyperolius parallelus pyrrhodictyon Hyperolius marmoratus taeniatus Hyperolius mariae Hyperolius reesi Hyperolius marmoratus marmoratus Hyperolius marmoratus swynnertoni Hyperolius nitidulus 1 Hyperolius spatzi Hyperolius nitidulus 2 Hyperolius viridiflavus ngorongoriensis Hyperolius viridiflavus ommatostictus Hyperolius viridiflavus pantherinus Hyperolius viridiflavus glandicolor Hyperolius viridiflavus pitmani Hyperolius viridiflavus ssp. Hyperolius viridiflavus destefanii Hyperolius viridiflavus variablilis Hyperolius koehleri Hyperolius tuberilinguis Hyperolius mosaicus Hyperolius endjami 2 Clade 2 Hyperolius endjami 1 Hyperolius cystocandicans Hyperolius jackie Hyperolius discodactylus Hyperolius lateralis Hyperolius castaneus Hyperolius frontalis Hyperolius platyceps Hyperolius raymondii Hyperolius langi Hyperolius kuligae Hyperolius ademetzi Hyperolius tanneri Hyperolius minitissimus Hyperolius burgessi Hyperolius spinigularis Hyperolius torrentis Hyperolius chlorosteus Hyperoliid Species Tree Analysis (ASTRAL-III) Hyperolius occidentalis Hyperolius sylvaticus Hyperolius baumanni 1,047 exons Hyperolius picturatus 561,180 bp Hyperolius bobirensis Hyperolius concolor concolor Hyperolius concolor guttatus Hyperolius concolor ibadanensis Branch Length Scale Hyperolius montanus Hyperolius pictus Hyperolius substriatus 2.0 Coalescent Units Hyperolius stictus Hyperolius mitchelli Hyperolius rubrovermiculatus Hyperolius quinquevittatus Node Support Key Hyperolius kivuensis 1 Hyperolius kivuensis 3 quartet support > 0.9; multilocus bootstrap > 70% Hyperolius kivuensis 2 Hyperolius balfouri balfouri quartet support < 0.9; multilocus bootstrap > 70% Hyperolius balfouri viridistriatus Hyperolius veithi quartet support > 0.9; multilocus bootstrap < 70% Hyperolius cinnamomeoventris 2 Hyperolius cinnamomeoventris 1 quartet support < 0.9; multilocus bootstrap < 70% Hyperolius olivaceus Hyperolius thomensis Hyperolius molleri.
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