bioRxiv preprint doi: https://doi.org/10.1101/398008; this version posted August 22, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Combinatorial recognition of clustered RNA elements by a multidomain RNA-binding protein, IMP3 Tim Schneider1,6, Lee-Hsueh Hung1,6, Masood Aziz2, Anna Wilmen1, Stephanie Thaum1, Jacqueline Wagner2, Robert Janowski3, Simon Müller4, Stefan Hüttelmaier4, Dierk Niessing3,5, Michael Sattler2*, Andreas Schlundt2*, and Albrecht Bindereif1* 1 Institute of Biochemistry, University of Giessen, 35392 Giessen, Germany 2 Center for Integrated Protein Science Munich (CIPSM) at Department of Chemistry, Technical University of Munich (TUM), 85747 Garching, Germany 3 Institute of Structural Biology, Helmholtz-Zentrum München, 85764 Neuherberg, Germany 4 Institute of Molecular Medicine, Section for Molecular Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany 5 Institute of Pharmaceutical Biotechnology, Ulm University, 89081 Ulm, Germany 6 These authors contributed equally to this work. * Correspondence should be addressed to: M.S. (
[email protected]), A.S. (
[email protected]), or A.B. (
[email protected]). 1 bioRxiv preprint doi: https://doi.org/10.1101/398008; this version posted August 22, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract How multidomain RNA-binding proteins recognize their specific target sequences, based on a combinatorial code, represents a fundamental unsolved question and has not been studied systematically so far.