Efficient Professional Protein and Antibody Platforms IGF2BP3 Antibody Basic information: Catalog No.: UPA62252 Source: Rabbit Size: 50ul/100ul Clonality: Polyclonal Concentration: 1mg/ml Isotype: Rabbit IgG Purification: Protein A affinity purified Useful Information: WB:1:1000-1:2000 Applications: IHC:1:50-1;200 FC:1:50-1:100 Reactivity: Human, Mouse, Rat Specificity: This antibody recognizes IGF2BP3 protein. Immunogen: Recombinant protein IGF-II mRNA-binding proteins (IMPs) bind RNA and influence RNA synthesis and metabolism. IMP-1, also known as coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein (CRD-BP) and VICKZ1; IMP-2 (IMP2, VICKZ2, p62); and IMP-3 (KOC1, VICKZ3) contain a unique combination of RNA recognition motifs and four hnRNP K homology domains. IMP-1 is abundant in embryonal tissues and is expressed in 81% of colon cancers, 73% of sarcomas and 58.5% of breast cancers. It recognizes Description: c-Myc, IGF-II and t mRNAs, and H19 RNA, and plays a major role in prolifera- tion of K-562 cells by an IGF-II-dependent mechanism. IMP-2 binds the 5' UTR of IGF-II mRNA and influences tumor cell growth, in which IMP-2 is as- sociated with apoptosis induced by tretinoin. IMP-3 knockdown by RNA in- terference decreases levels of IGF-II protein without affecting IGF-II, c-Myc, or β Actin mRNA and H19 RNA levels. IMP-3 is a marker for carcinomas and high-grade dysplastic lesions of pancreatic ductal epithelium. Uniprot: O00425(Human) Q9CPN8(Mouse) BiowMW: 63 kDa Buffer: 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data:
Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Western blot analysis of IMP-3 on Hela (1) and 293T (2) cell lysate using anti-IMP-3 antibody at 1/2,000 dilution.
Immunohistochemical analysis of paraf- fin-embedded rat brain tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraf- fin-embedded human placental tissue using an- ti-IMP-3 antibody. Counter stained with hema- toxylin.
Immunohistochemical analysis of paraf- fin-embedded mouse testis tissue using an- ti-IMP-3 antibody. Counter stained with hema- toxylin.
Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]