Hypoxia stimulates glucose metabolism of eutopic and ectopic endometrial stromal cells from women with endometriosis

Abstract ID : 1669 Soumis par : Yun-Dong MAO Le 2016-03-14 14:08:30 Nom de la catégorie : SEUD CONGRESS Typologie : Communication orale / Oral communication Statut : validé Autorisation de diffusion : Yes/Oui

------Title: Hypoxia stimulates glucose metabolism of eutopic and ectopic endometrial stromal cells from women with endometriosis Authors: Xiadi Wu, Xiaochen Qi, Juan Qu, Yanxin Zhang, Fuxin Wang, Manxing Xie, Li Shu, Shiwen Jiang, Yundong Mao*, Yugui Cui, Jiayin Liu

* Corresponding author. E-mail address: [email protected] (Y Mao).

Abstract: Introduction: The aim of the study was to examine the glucose metabolism of endometrial stromal cells under hypoxia condition as found in the peritoneal cavity, and explore their potential role for the development of endometriosis. Materials and Methods: An in vitro study was conducted in stromal cells isolated from endometrium of women with (EU) or without (NE) endometriosis as well as from endometrioma tissues (EC). We determined glucose consumption, lactate generation, adenosine triphosphate (ATP) production and protein expression of hypoxia-inducible factor-1? (HIF- 1?)?hexokinase 2 (HK2) and lactic dehydrogenase A (LDHA) of stromal cells to characterize glucose metabolism. Moreover, mitochondrial membrane potential (MMP) and protein expression of pyruvate dehydrogenase kinase1 (PDK1) were also measured to assess mitochondrial function at normal (21% O2) and low (1% O2) oxygen condition. Cell apoptosis and invasion activities were examined as downstream events. Results: We found that EC consumed the most glucose and produced the most lactate and ATP while mitochondrial membrane potential was the lowest in atmospheric oxygen (21% O2) among the three groups of stromal cells. Compared to EC and NE, EU exhibited the strongest invasion activity, used more glucose and generated more lactate with reduced apoptosis rate. In hypoxia circumstance, all three types of cells showed increase ATP production and higher invasion activity but little change in apoptosis. Conclusions: These results suggest that hypoxia may promote change in energy metabolism and the invasion of the endometrial cells at ectopic sites, and ultimately, facilitate the development of endometriosis. ------

Mots clefs : Endometriosis; Glucose metabolism; Hypoxia; Invasion Auteurs : Références : , , ,

Auteurs

Xia-Di WU 1, Xiao-Chen QI 2, Juan QU 3, Yan-Xin ZHANG 4, Fu-Xin WANG 5, Man-Xin XIE 6, Shi-Wen JIANG 7, Li SHU 1, Yun-Dong MAO 1, Yu-Gui CUI 1, Jia-Yin LIU 1,

1. Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Medical University/ Province Hospital, Nanjing, 2. Department of Obstetrics and Gynecology, The First Clinical Medical College of Three Gorges University, Yichang Central People?s Hospital, Yichang, CHINA 3. Department of Obstetrics and Gynecology, Taian Central Hospital, Taian, CHINA 4. Department of Obstetrics and Gynecology, Clinical Medical College of University, Subei People?s Hospital of Jiangsu, Yangzhou, CHINA 5. Center of Reproductive and Genetic Medicine, Affiliated Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, CHINA 6. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, CHINA 7. Department of Obstetrics and Gynecology, Memorial Health Hospital, Savannah, UNITED STATES

Auteurs (raw format)

WU Xia-Di - email : [email protected] Etablissement : The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital Service : Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine Ville : Nanjing Pays : CHINA Présentateur : Oui QI Xiao-Chen - email : [email protected] Etablissement : The First Clinical Medical College of Three Gorges University, Yichang Central People?s Hospital Service : Department of Obstetrics and Gynecology Ville : Yichang Pays : CHINA Présentateur : Non QU Juan - email : [email protected] Etablissement : Taian Central Hospital Service : Department of Obstetrics and Gynecology Ville : Taian Pays : CHINA Présentateur : Non ZHANG Yan-Xin - email : [email protected] Etablissement : Clinical Medical College of Yangzhou University, Subei People?s Hospital of Jiangsu Service : Department of Obstetrics and Gynecology Ville : Yangzhou Pays : CHINA Présentateur : Non WANG Fu-Xin - email : [email protected] Etablissement : Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital Service : Center of Reproductive and Genetic Medicine Ville : Suzhou Pays : CHINA Présentateur : Non XIE Man-Xin - email : [email protected] Etablissement : The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital Service : Department of Obstetrics and Gynecology Ville : Nanjing Pays : CHINA Présentateur : Non JIANG Shi-Wen - email : [email protected] Etablissement : Memorial Health Hospital Service : Department of Obstetrics and Gynecology Ville : Savannah Pays : UNITED STATES Présentateur : Non SHU Li - email : [email protected] Etablissement : The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital Service : Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine Ville : Nanjing Pays : CHINA Présentateur : Non MAO Yun-Dong - email : [email protected] Etablissement : The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital Service : Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine Ville : Nanjing Pays : CHINA Présentateur : Non CUI Yu-Gui - email : [email protected] Etablissement : The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital Service : Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine Ville : Nanjing Pays : CHINA Présentateur : Non LIU Jia-Yin - email : [email protected] Etablissement : The First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital Service : Center of Clinical Reproductive Medicine, State Key Laboratory of Reproductive Medicine Ville : Nanjing Pays : CHINA Présentateur : Non