RESEARCH ARTICLE Ablation of STAT3 in Purkinje cells reorganizes cerebellar synaptic plasticity in long-term fear memory network Jeong-Kyu Han1,2,3,4†, Sun-Ho Kwon3,5,6†, Yong Gyu Kim1,6, Jaeyong Choi6,7, Jong-Il Kim6,7, Yong-Seok Lee1,4,6, Sang-Kyu Ye1,5,6*, Sang Jeong Kim2,3,4,6* 1Department of Physiology, Seoul National University College of Medicine, Seoul, Republic of Korea; 2Department of Brain and Cognitive Sciences, Seoul National University Graduate School, Seoul, Republic of Korea; 3Memory Network Medical Research Center, Seoul National University College of Medicine, Seoul, Republic of Korea; 4Neuroscience Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea; 5Department of Pharmacology, Seoul National University College of Medicine, Seoul, Republic of Korea; 6Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea; 7Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Republic of Korea Abstract Emotional memory processing engages a large neuronal network of brain regions including the cerebellum. However, the molecular and cellular mechanisms of the cerebellar cortex modulating the fear memory network are unclear. Here, we illustrate that synaptic signaling in cerebellar Purkinje cells (PCs) via STAT3 regulates long-term fear memory. Transcriptome analyses *For correspondence: revealed that PC-specific STAT3 knockout (STAT3PKO) results in transcriptional changes that lead to
[email protected] (S-KY); an increase in the expression of glutamate receptors. The amplitude of AMPA receptor-mediated
[email protected] (SJK) excitatory postsynaptic currents at parallel fiber (PF) to PC synapses was larger in STAT3PKO mice †These authors contributed than in wild-type (WT) littermates.