SANTA CRUZ BIOTECHNOLOGY, INC. TRAFD1 shRNA (h) Lentiviral Particles: sc-63145-V

BACKGROUND APPLICATIONS TRAFD1 (TRAF-type zinc finger domain containing 1), also known as FLN29, TRAFD1 shRNA (h) Lentiviral Particles is recommended for the inhibition of is a novel 582 amino acid that acts as a lipopolysaccharide (LPS)- TRAFD1 expression in human cells. and interferon (IFN)-inducible negative regulator, and may also play a role in endotoxin tolerance. TRAFD1 also interacts with TRAF6 to negatively SUPPORT REAGENTS regulate toll-like receptor (TLR) signaling. TRAFD1 contains one TRAF-type Control shRNA Lentiviral Particles: sc-108080. Available as 200 µl frozen zinc finger and contains multiple phosphoserine and phosphothreonine viral stock containing 1.0 x 106 infectious units of virus (IFU); contains an residues. The encoding TRAFD1 maps to human 12, shRNA construct encoding a scrambled sequence that will not lead to the which encodes over 1,100 and comprises approximately 4.5% of the specific degradation of any known cellular mRNA. . is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest RT-PCR REAGENTS dysplasia, Noonan syndrome and trisomy 12p, which causes facial develop- mental defects and seizure disorders. Semi-quantitative RT-PCR may be performed to monitor TRAFD1 knockdown using RT-PCR Primer: TRAFD1 (h)-PR: sc-63145-PR REFERENCES (20 µl). Annealing temperature for the primers should be 55-60° C and the extension temperature should be 68-72° C. 1. Cao, Z., et al. 1996. TRAF6 is a signal transducer for interleukin-1. Nature 383: 443-446. BIOSAFETY 2. Delgado Carrasco, J., et al. 2001. Achondrogenesis type II-hypochondroge- Lentiviral particles can be employed in standard Biosafety Level 2 tissue nesis: radiological features. Case report. An. Esp. Pediatr. 55: 553-557. culture facilities (and should be treated with the same level of caution as 3. Yokoyama, T., et al. 2003. A case of Kniest dysplasia with retinal detach- with any other potentially infectious reagent). Lentiviral particles are repli- ment and the mutation analysis. Am. J. Ophthalmol. 136: 1186-1188. cation-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. 4. Mashima, R., et al. 2005. FLN29, a novel interferon- and LPS-inducible gene acting as a negative regulator of toll-like receptor signaling. J. Biol. STORAGE Chem. 280: 41289-41297. Store lentiviral particles at -80º C. Stable for at least one year from the 5. Nousiainen, M., et al. 2006. Phosphoproteome analysis of the human date of shipment. Once thawed, particles can be stored at 4º C for up to mitotic spindle. Proc. Natl. Acad. Sci. USA 103: 5391-5396. one week. Avoid repeated freeze thaw cycles. 6. Sauvé, S., et al. 2008. NMR assignment of the N-terminal TRAF-like RING zinc finger domain of human FLN29. Biomol. NMR Assign. 2: 33-36. PROTOCOLS See our web site at www.scbt.com for detailed protocols and support CHROMOSOMAL LOCATION products. Genetic : TRAFD1 (human) mapping to 12q24.13.

PRODUCT TRAFD1 shRNA (h) Lentiviral Particles is a pool of concentrated, transduction- ready viral particles containing 3 target-specific constructs that encode 19- 25 nt (plus hairpin) shRNA designed to knock down gene expression. Each vial contains 200 µl frozen stock containing 1.0 x 106 infectious units of virus (IFU) in Dulbecco’s Modified Eagle’s Medium with 25 mM HEPES pH 7.3. Suitable for 10-20 transductions. Also see TRAFD1 siRNA (h): sc-63145 and TRAFD1 shRNA Plasmid (h): sc-63145-SH as alternate gene silencing products.

RESEARCH USE The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product and all replicates and derivatives for research purposes conducted by the buyer in his laboratory only (whether the buyer is an academic or for-profit entity). The buyer cannot sell or other- wise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party, or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com