Dr. Anie Y. Assistant Professor (Biochemistry) School of Biosciences, Mahatma Gandhi University, Kottayam E-mail – [email protected] . [email protected] Tel: 9947090370

CURRICULUM VITAE

Date of Birth: May 5, 1980 Citizenship: Indian

Address: Prakrithi Amalagiri P O Kottayam Kerala, India

PROFESSIONAL EXPERIENCE

• Assistant Professor : 2006 – till date, Asst. Professor in Biochemistry ,School Of Biosciences, Mahatma Gandhi University, Kottayam, 686560.

• PhD : 2003-2009, Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCTIMST), Thiruvananthapuram, Kerala, India.

• UGC-JRF(NET) : 2002

• M.SC Biochemistry : 2003, School of Biociences MG University, First class, Third rank .

RESEARCH INTEREST

• Immunobiology

Research in the lab focusses on the behaviour of immune cells like neutrophils and monocytes during metabolic diseases like Diabetes and Atherosclerosis. In addition to that, contribution of NEtosis/Etosis, a recently discovered mode of defence mechanism in both neutrophils and monocytes, on the pathogenesis of Atherosclerosis via histone citrullination is also envisaged. Hormonal influence of PAD-4 gene activity which is necessary for NETosis on disease progression is another area of interest.

• Natural Product Research

Plants might serve as an important source for the isolation of potential lead molecules for drug development. Likewise, some of the bacterial/fungal strains may also produce potential therapeutic molecules. In the lab, we are looking for new Aldose Reductase Inhibitor molecules from plants and fibrinolytic enzymes from microorganisms. Inhibition of the Aldose Reductase enzyme of the polyol pathway is considered efficient in delaying or preventing development of secondary diabetic complications. Thus, identification and analysis of these potentially therapeutic candidates for efficacy, cytotoxicity and mode of action is useful in developing lead molecules. So the isolation, characterization and interaction studies of these molecules with the target enzymes or molecules may provide information essential for their utilization as drugs in the management of these clinical complications or diseases.

FUNDED PROJECTS

SL TOPIC Year VALUE STAT FUNDING . US AGENCY N O. 1. Chemotactic response of human 2010-2012 1,24000 Finis UGC – phagocytic cells (polymorphonuclear hed MINOR leucocytes and monocytes) in RESEARC tuberculosis patients.[F.NO. H 37/599/2009(SR)] PROJECT 2. Nattokinase – fibrinolytic and anti- 2013-2014 4,73,000 Finis KSCSTE( hypertensive enzyme from novel hed YIPB) bacterialisolates. (NO.03/YIPB/KBC/2012/CSTE) 3. Immunomodulatory effect of beta – 2014-2017 13,00000 Ongo KSCSTE(B glucan on neutrophil functions in ing ack to lab Diabetes mellitus. programme (NO 1368/2014/KSCSTE) -Scientist mentor) 4. Novel aldose reductase inhibitors 2012-2016 NA Finis Project III from natural sources. hed OF DBT (No.BT/P44800/INF/22/152/2012) MSUB Interdepart mental project

PUBLICATIONS

Publications in journal:

• Hazeena VN, Sruthi CR, Soumiya CK, Haritha VH, Jayachandran K, Anie Y . Vernonia anthelmintica (L.) Willd. Prevents Sorbitol Accumulation through Aldose Reductase Inhibition. Scholars Academic Journal of Biosciences: 4(10A); 787-795. • Surya PR, Liji L, Jayachandran K, Anie Y. Bacteria from South Indian Fermented Foods and Food- Waste Dump Sites as Sources of Fibrinolytic Enzymes. Imperial Journal of interdisciplinary research 2016: 2(7); 528- 536. • Anie Y , Sumi S, Varghese P, Madhavi L G K, Sathish M, Radhakrishnan V V. Diagnostic approaches in patients with tuberculous pleural effusion. Diagn Microbiol Infect Dis 2007: 59; 389-394.( Impact factor-2.457) Books chapter:

• Y Anie , V N Hazeena, V H Haritha and V S Binchu, Multi-faceted anti-diabetic actions of aldose reductase inhibitors. Advances in Functional Foods for Human Health. (Accepted for publication on 28-10-2016).

• V H Haritha, V S Binchu, V N Hazeena and Y Anie , Xylitol: A potent immunomodulator. Advances in Functional Foods for Human Health. (Accepted for publication on 28-10- 2016).

• Mathai A, Sumi M G, Sumi S, Anie Y and Radhakrishnan V V, Immunology of neurotuberculosis. In Reviews in Neurology 2009 Ed Tally A B, Singh G. pg 265-294.

Papers published in conference Proceedings:

1. Anie Y , Sumi S, Radhakrishnan V V. Diagnostic utility of serum IgG antibody to lipid antigens of mycobacterium tuberculosis in patients with pulmonary tuberculosis. In the proceedings of the 19th Kerala Science congress held at Kannur from 29-31 January, 2007. pp 268-269

2. Anie.Y , Sumi. S, Radhakrishnan V V. Diagnosis of tuberculous lymphadenitis – Molecular and Immunological approaches, In the proceedings of the 18th Kerala Science congress held at Thiruvananthapuram from 29-31 January, 2006. pp 471-472

3. Sumi S, Anie Y , Radhakrishnan V V, Diagnostic approaches in pleural tuberculosis. In the proceedings of the 18th Kerala Science congress held at Thiruvananthapuram from 29-31 January, 2006. pp 444-445.

4. Haritha V H, Neethi Raj P and Anie Y . Xylitol Action on impaired Neutrophil function Associated with Alcoholism. Proceedings of the National seminar on life style and Diseases. School of Biosciences, Mahatma Gandhi University. Jan 19 and 20, 2012. P. P 22-24. 5. P. Neethi Raj, V.H. Haritha and Y. Anie , Hyperglycemia and/or hyperinsulinemia conditions affect neutrophil functions indirectly, In the proceeding of Swadesi science congress 6-8 November 2012, 350-357. 6. Hazeena V.N., Jayachandran K. Suria P R and Anie Y . (2014, January 28-31) Screening and selection of Medicinal plants for aldose reductase inhibition potential of Centratherum anthelminticum. In VN Rajasekharan Pillai (Eds.), Proceedings of the 26th Kerala Science Congress. 28-31 January (pp-123-124). Wayanad, Kerala. 7. Binchu V Shaji, Neethi Raj, Haritha V H, Anie Y . (2014, November 6-8). Modulation of PMN (polymorphonuclear leukocyte) secretion and its impact on monocyte function in the presence of hyperglycemia and/or hyperinsulinemia. In Dr. R. Jayaprakash (Eds.). Proceedings. 24th swadeshi science congress. 6-8 November (pp-661-667). Thunchath Ezhuthachan Malayalam University, Tirur, Malappuram district, Kerala. 8. Surya P Rajendran, Liji L, Hazeena V. N., Jayachandran K., and Anie Y . (2014, November 6-8). Fibrinolytic enzyme production by bacillus species. In Dr. R. Jayaprakash (Eds.). Proceedings. 24th swadeshi science congress. 6-8 November (pp- 661-667). Thunchath Ezhuthachan Malayalam University, Tirur, Malappuram district, Kerala. 9. Rose Kurian and Anie Y . (2014, November 6-8). In vitro analysis of the ethyl acetate fraction of Vernonia anthelminticum . In Dr. R. Jayaprakash (Eds.). Proceedings. 24th swadeshi science congress. 6-8 November (pp-661-667). Thunchath Ezhuthachan Malayalam University, Tirur, Malappuram district, Kerala. 10. Neethi Raj P, Haritha V. H and Anie Y . (2015, January 27-29). Neutrophil extracellular trap formation during hyperglycemia. In Mara Pandiyan (Eds.), Proceedings of the 27th Kerala Science Congress. 27-29 January (pp-123-124). Alappuzha, Kerala. 11. Surya P Rajendran, Liji L, Jayachandran K., Anie Y . (2015, January 27-29). Fibrinolytic enzyme from bacillus species: culture optimisation and enzyme purification. In Mara Pandiyan (Eds.), Proceedings of the 27th Kerala Science Congress. 27-29 January (pp- 123-124). Alappuzha, Kerala. 12. Hazeena V.N., Jayachandran K. and Anie Y ., (2015, April 25). International symposium on Phytochemistry (ISP-2015), Ethyl acetate fraction of Vernonia anthelmintica: HPTLC Fingerprinting and enzyme kinetic study of the active ARI constituents, in V. Anthelmintica 13. Haritha V.H, Seena P, Anie Y , Role of monocytes in the clearance of NET (Neutrophil extracellular trap) and apoptotic bodies. In the proceedings of 28th Kerala Science Congress held at University of Calicut, Malappuram, 28- 30 January 2016, pp. 1447- 1458. 14. Hazeena V.N., Soumiya C. K., Anie Y ., Comparatitive analysis of butanol: acetonitrile and acetonitrile fractions of Vernonia anthelmintica for possible aldose reductase inhibitory constituents. In the proceedings of 28th Kerala Science Congress held at University of Calicut, Malappuram, 28- 30 January 2016, pp. 1680-1691. 15. Hazeena V.N., Haritha V. H. , Jayachandran K., Anie Y ., Ethyl acetate fraction of Vernonia anthelmintica (L.) Willd contains Aldose reductase inhibitory molecules. In the proceedings of National Seminar on Plant Science Technology for Sustainable World, 25th and 26 th February, 2016, pp. 55-59. 16. Shahana Shaji, Binchu V Shaji, Haritha V H, Anie Y . Glucose and Insulin as modulators of NETosis. In proceedings of 26 th Swadeshi Science congress held at CMFRI, Cochin, 7-9 November 2016, pp. 159-160. 17. Surya P Rajendran, Jayachadran K, Anie Y. Liposome: A novel vehicle for the administration of fibrinolytic enzymes. . In proceedings of 26 th Swadeshi Science congress held at CMFRI, Cochin, 7-9 November 2016, pp. 209.

FELLOWSHIPS/AWARD

UGC JRF Fellowship 2002.

Dr. S. S. Misra award for the best work for the year 2006-2007 by the National Academy of Medical Sciences (India), New Delhi.

REFRESHER/ORIENTATION COURSES ATTENDED

1. Refresher Course in Life Sciences, UGC-Human Resource Development Center, University of Kerala, Kariavattom, 25 th Jun to 15 th jul 2015

2. Short- term course for Research Guides in Sciences, UGC-Academic Staff College, University of Kerala, Kariavattom, 5 th Nov to 9 th Nov 2012

3. Refresher course, UGC-Academic Staff College, University of Calicutt, 4 th Aug to 24 th Aug 2011.

4. Orientation Programme, UGC-Academic Staff College, University of Kerala, 24 th Sep 21 st Oct 2010.

CONFERENCE

1. Anie Y , Sumi S, Radhakrishnan V V. Diagnostic utility of serum IgG antibody to lipid antigens of mycobacterium tuberculosis in patients with pulmonary tuberculosis. At the 19th Kerala Science congress held at Kannur from 29-31 January, 2007. pp 268-269

2. Anie.Y , Sumi. S, Radhakrishnan V V. Diagnosis of tuberculous lymphadenitis – Molecular and Immunological approaches. At the 18th Kerala Science congress held at Thiruvananthapuram from 29-31 January, 2006. pp 471-472 3. Sumi S, Anie Y , Radhakrishnan V V, Diagnostic approaches in pleural tuberculosis. At the 18th Kerala Science congress held at Thiruvananthapuram from 29-31 January, 2006. pp 444-445.

4. Haritha V H, Neethi Raj P and Anie Y . Xylitol Action on impaired Neutrophil function Associated with Alcoholism. At the National seminar on life style and Diseases. School of Biosciences, Mahatma Gandhi University. Jan 19 and 20, 2012. P. P 22-24.

5. P. Neethi Raj, V.H. Haritha and Y. Anie , Hyperglycemia and/or hyperinsulinemia conditions affect neutrophil functions indirectly, At the Swadesi science congress 6-8 November 2012, 350-357. 6. P. Neethi Raj, Binchu V Shaji, Haritha V H, Anie Y Neutrophil extracellular DNA trap formation is modulated by Hyperglycemia/ Hyperinsulinemia Condition.and International conference on Research Methodology and Scientific writing held at M G University, jointly organised by School of Behavioural Sciences of M G University and Confederation of Epidemological Associations from 20-22 December 2013. 7. V N Hazeena, K Jayachandran P. R Suria, Y Anie . Aldose reductase inhibition by Medicianl plants.and International conference on Research Methodology and Scientific writing held at M G University, jointly organised by School of Behavioural Sciences of M G University and Confederation of Epidemological Associations from 20-22 December 2013. 8. Binchu V Shaji, Neethi Raj, Haritha V H, Anie Y . (2014, November 6-8). Modulation of PMN (polymorphonuclear leukocyte) secretion and its impact on monocyte function in the presence of hyperglycemia and/or hyperinsulinemia. At the 24th swadeshi science congress. 6-8 November (pp-661-667). Thunchath Ezhuthachan Malayalam University, Tirur, Malappuram district, Kerala. 9. Surya P Rajendran, Liji L, Hazeena V. N., Jayachandran K., and Anie Y . (2014, November 6-8). Fibrinolytic enzyme production by bacillus species. At the 24th swadeshi science congress. 6-8 November (pp-661-667). Thunchath Ezhuthachan Malayalam University, Tirur, Malappuram district, Kerala. 10. Rose Kurian and Anie Y . (2014, November 6-8). In vitro analysis of the ethyl acetate fraction of vernonia anthelminticum . At the 24th swadeshi science congress. 6-8 November (pp-661-667). Thunchath Ezhuthachan Malayalam University, Tirur, Malappuram district, Kerala. 11. Neethi Raj P, Haritha V. H and Anie Y . (2015, January 27-29). Neutrophil extracellular trap formation during hyperglycemia. At the 27th Kerala Science Congress. 27-29 January (pp-123-124). Alappuzha, Kerala. 12. Hazeena V.N., Soumiya C. K., Anie Y . (2016 January 28- 30). Comparatitive analysis of butanol: acetonitrile and acetonitrile fractions of Vernonia anthelmintica for possible aldose reductase inhbitory constituents. At the 28 th Kerala Science Congress. 1680-1691. 13. Haritha V.H., Seena P., Anie Y . (2016 January 28- 30). Role of monocytes in the clearence of net (neutrophil extracellular trap) and apoptotic bodies. At the 28 th Kerala Science Congress. 1447-1458. 14. Hazeena V.N., Haritha V. H. , Jayachandran K., Anie Y . (2016 February 25- 26 ). Ethyl acetate fraction of Vernonia anthelmintica (L.) Willd contains aldose reductase inhibitory molecules. At the National Seminar on Plant Science Technology for Sustainable World. 55-59. 15. Sneha K.R, Sumitha N.S. Mohammed Mustafa C.P, Anie Y and sailaja G.S. Biofunctional cellulose nanofibres trom Agove sisolano:A potential precursor for biomedical applications. In the proceedings of CUSAT-NUS joint International conference on Biotechnology and Neuroscience held at Cochin on 19-21 Dec 2016. Pg 55. 16. Sumitha N S, Veena V, Anie Y and sailaja G.S. Magnetic Biodegradable Microspheres for Targeted Therapy. In the proceedings of CUSAT-NUS joint International conference on Biotechnology and Neuroscience held at Cochin on 19-21 Dec 2016. Pg 56. 17. Sirajunnisa P. Anjali S.B. , Anie Y . , Yamuna A., Sobhi Daniel and Sailaja G.S. Synthesis of Novel Biocompatible Fluorophore-Conjugated Polymer Derivatives for Bioimaging applications. In the proceedings of CUSAT-NUS joint International conference on Biotechnology and Neuroscience held at Cochin on 19-21 Dec 2016. Pg 54.

POSTER PRESENTATIONS

1. Surya P Rajendran, Liji L, Jayachandran K., Anie Y . (2015, January 27-29). Fibrinolytic enzyme from bacillus species: culture optimisation and enzyme purification at the 27th Kerala Science Congress. 27-29 January . Alappuzha, Kerala. 2. Hazeena V.N., Jayachandran K. and Anie Y .,(2015, April 25), Ethyl acetate fraction of Vernonia anthelmintica: HPTLC Fingerprinting and enzymekinetic study of the active ARI constituents at International symposium on Phytochemistry (ISP-2015) and Prof. Dr. A. Hisham endowment award ceremony, Trivandrum.

PRESENTATIONS AND INVITED TALKS

• At DST sponsored INSPIRE Science camp, Union Christian College, Aluva, May27-31, 2013.

• At UGC sponsored national seminar on ‘Basic and advanced biochemical techniques: Relevance in interdisciplinary research scenario’; Bishop Kurialacherry college for women, Kottayam, 3-4 Sept 2013.

• At UGC sponsored National seminar on ‘Modern approaches for the management of Diabetes mellitus and the ethical issues associated with drug trials and banned drugs’; N.S.S College, Pandalam, 11-13 Feb 2015.

SEMINARS CONDUCTED

• National seminar on life style and Diseases. School of Biosciences, Mahatma Gandhi University. Jan 19 and 20, 2012.

• Joint Convener- National workshop on “ Advanced statistical tool & softwares for biological research”. School of Biosciences, Mahatma Gandhi University. Sep27-Oct 1 2016.

• Executive committee member- Two day National seminar on Recent trends in applied Life Sciences. School of Biosciences, Mahatma Gandhi University. Mar 15 and 16, 2017. RESEARCH TEAM

1. Neethi Raj P. (Women Scientist – Back to lab Programme, KSCSTE)-Role of neutrophil secretions in modulating neutrophil and monocyte functions during hyperglucose and hyperinsulin conditions.

2. Hazeena V.N. (SRF, DBT-MSUB,IPLSARE)- Aldose reductase inhibitory potential of Vernonia anthelminitica (L.) Willd

3. Surya P. Rajendran (Research fellow)- )- Fibrinolytic enzyme from Bacillus species: Characterization and application studies -Thesis submitted

4. Haritha V.H. (Research fellow)- Citrullinated Histone of Neutrophil Extracellular Trap (NET) in Atherosclerotic Plaque Formation.

5. Binchu V. Shaji (Research fellow) – Hormonal Regulation of Peptidyl Arginine Deiminase-4 (PAD-4).

6. Prince Thomas (Research fellow)-In vitro and In vivo Evaluation of Aldose Reductase Inhibitors.

STUDENTS ACHIEVEMENT

• Best paper award in the National seminar on Plant Science Technology for Sustainable World, conducted at the School of Biosciences, Mahatma Gandhi University on - Hazeena V. N.

• Back to lab programme (KSCSTE) award to Neethi Raj P ; (Scientist Mentor – Dr. AnieY.)

• Best Paper Award in National seminar on life style and Diseases, School of Biosciences, Mahatma Gandhi University. Jan 19 and 20, 2012- Haritha V.H.

• DBT MSUB IPLSARE research fellowship – Hazeena V.N.

OTHER DETAILS AND POSITIONS Member- Board of studies, M.Sc Microbiology, Mahatma Gandhi University

Member- Expert committee, M.Sc Biochemistry, Mahatma Gandhi University

Member- Board of studies, Biotechnology, Kannur University

Member – Board of question paper setters Kannur University.

Member – Board of question paper setters, University Of Calicut.

Member – Board of question paper setters University Of Kerala.

Member- Board of examination, M.Sc Biochemistry, Mahatma Gandhi University.

Member – Animal ethical committee, M G University, Kottayam.

Member – Biosafety committee, M G University, Kottayam

Faculty –in-charge of Animal House, M G University, Kottayam

DETAILS OF Ph.D, M.Phil AND M..Sc. RESEARCH TRAININGS

Ph.D dissertations guided

1. Surya P Rajendran ( 2017)- Fibrinolytic enzyme from Bacillus species: Characterization and application studies- Thesis Submitted

M.Phil dissertations guided

1. Haritha V.H. (2012)- Action of xylitol on impaired neutrophil functions associated with alcoholism

2. Dhanya R (2012) - Honey as a wound healing agent in diabetes: An investigation into its antibacterial and immune enhancing abilities.

3. Binchu V Shaji (2014) - An in vitro analysis of the modulation of PMN secretion and its impact on monocyte function in the presence of hyperglycemia and / or hyperinsulinemia. 4. Seena P (2015)- Monocytes In the clearance of NET (Neutrophil Extracellular Trap) and apoptotic neutrophil.

5. Sivaprabha. V. Nair (2017)- Cytotoxic effects of different concentrations of arsenic trioxide – an acute promyelocytic leukemia drug- Co-guide

M.Sc dissertations guided

1. Vineeth C.A. (Aug 2011) - Study on chemotactic response of human polymorphonuclear cells in tuberculosis patients.

2. Remya T.V. (Aug 2011)- Study on chemotactic response of human peripheraL blood mononuclear cells(PBMCs) in the presence of tuberculosis sera.

3. Resmi N. B (sep 2012) - Potential of honey as an antioxidant in diabetes; an in vitro study.

4. Susanna Maria (sep 2012, Co-guide ) - Toxicity and immunopotential studies of xylitol.

5. Bhagyalakshmi P (oct 2013) - Aldose Reductase Inhibitory Potential Of Centratherum anthelminticum.

6. Liji L (Sep 2014) - Fibrinolytic enzymes from Bacillus species: Bacterial identification, culture optimization and enzyme isolation.

7. Sruthy C R (Sep 2014)- Aldose reductase inhibition of the butanol fraction of Centratherum anthelminticum : kinetic analysis and LC-MSQ-ToF analysis of bioactive HPLC fraction.

8. Neethu M (Oct 2015) - Functional analysis of neutrophils from Diabetes mellitus patients.

9. Soumiya C.K (Oct 2015) - Aldose reductase inhibitory potential of Butanol - acetonitrile and acetonitrile fractions of Vernonia anthelminiticum.

10. Shahaha Shaji (Oct 2016) - ROS and PAD-4 independent vital NETosisis modulated by glucose