Aureobasidium Tremulum Fungal Planet Description Sheets 383

382 Persoonia – Volume 42, 2019

Aureobasidium tremulum Fungal Planet description sheets 383

Fungal Planet 907 – 19 July 2019 Aureobasidium tremulum Inamdar, Roh. Sharma & Adhapure, sp. nov.

Etymology. Named after the shaking and trembling behaviour of the Typus. India, Aurangabad, Maharashtra, laboratory contaminant, July yeast when observed under a light microscope (Latin tremulum= shaking, 2016, A. Inamdar (holotype MCC 1683 preserved as metabolically inac- trembling). tive strain, ITS and LSU sequences GenBank MK503657 and MK503660, MycoBank MB829941). Classiﬁcation — Aureobasidiaceae, Dothideales, Dothideo mycetes. Notes — An initial BLASTn similarity search using the LSU region sequence in the NCBI type sequences nucleotide data- Initial growth as creamy white colonies on potato dextrose base showed the highest similarity to A. lini CBS 125.21 (Gen- agar, later turning brown to dark brown. Colonies appear to be Bank MH866211; 98 % identity, 99 % query cover) followed by rough and dry. Each colony is round with a convex elevation A. melanogenum strain CBS 105.22 (GenBank MH866219; from a cross-sectional viewpoint and the edges appear to be 98 % identity; query coverage 97 %). The BLASTn similarity undulated. Growth is optimal on Saboraud dextrose agar (SDA). search in the NCBI type sequences database using the ITS Colonies on nutrient agar did not become dark brown. Cells sequence showed the highest similarity to Kabatiella bupleuri are generally oblong-shaped with very few cells assuming an CBS 131304 (GenBank NR_121524; 95 % identity, 100 % irregular shape. Budding occurs frequently. The average size query coverage) followed by Aureobasidium iranianum CCTU of mature, non-budding cells is 2.8 × 6.4 µm. Sexual reproduc 268 (GenBank KM093738; 95 % identity, 99 % query coverage) tion was not observed. Pseudohyphal formation not observed. and A. melanogenum CBS 105.22 (GenBank NR_159598, Optimal growth occurred at 20–25 °C, with some growth at 95 % identity, 99 % query coverage). The neighbour-joining 5–15 °C. The following carbon compounds are assimilated: (NJ) phylogenetic analyses of ITS and LSU rRNA gene regions D-glucose, L-arabinose, D-xylose, D-maltose, D-saccharose, were done using sequences of other species of Aureobasidium. D-Trehalose, D-melezitose, D-rafﬁnose. No growth observed The phylogenetic tree topology clearly shows that the present with glycerol, calcium-2-keto-gluconate, L- lactose while weak strain UN-1 is novel and does not cluster with any known spe- assimilation was observed for adonitol, xylitol, D-galactose, cies of the genus. The phylogenetic analysis based on the ITS methyl-alpha- D-glucopyranoside and D-cellobiose. alignment shows that it forms a sister branch to A. thailandense Habitat — Aureobasidium tremulum was isolated as a culture NRRL 58543 (GenBank JX462675) and A. mangrovei IBRC- contaminant in the laboratory of Department of Biotechnology M-30266 (GenBank KY089087). In the phylogenetic analysis and Microbiology of Vivekanand Arts, Sardar Dalipsingh Com- based on the LSU alignment, it does not group with known merce and Science College, Aurangabad. species but was placed at equal evolutionary distance with Distribution — India (Aurangabad, Maharashtra). A. caulivorum CBS 242.64 (GenBank FJ150944).

Aureobasidium proteae CBS 114273 (KT693731) LSU region phylogenetic tree Aureobasidium pullulans CBS 100280 (FJ150910) Kabatiella microsticta CBS 342.66 (KT693743) Aureobasidium proteae CBS 114273 (KT693731) LSU region phylogenetic tree 72 Aureobasidium proteae CBS 111973 (KT693732) Aureobasidium pullulans CBS 100280 (FJ150910) Aureobasidium pullulans CBS 146.30 (FJ150902) Kabatiella microsticta CBS 342.66 (KT693743)

Aureobasidium pullulans CBS 584.75 (FJ150906) 72 Aureobasidium proteae CBS 111973 (KT693732) 96 Aureobasidium pullulans EXF-915 (FJ150911) Aureobasidium pullulans CBS 146.30 (FJ150902) Aureobasidium pullulans CBS 584.75 (FJ150906) 58 Aureobasidium pullulans CBS 100524 (FJ150905) 96 Aureobasidium pullulans EXF-915 (FJ150911) Kabatiella lini CBS 125.21 (FJ150897) 58 Aureobasidium pullulans CBS 100524 (FJ150905) Aureobasidium namibiae CBS 147.97 (FJ150875) Kabatiella lini CBS 125.21 (FJ150897) Aureobasidium melanogenum CBS 621.80 (FJ150885) Aureobasidium namibiae CBS 147.97 (FJ150875) 90 Aureobasidium melanogenumdH 12640 (FJ150889) 71 Aureobasidium melanogenum CBS 621.80 (FJ150885) Aureobasidium melanogenum CBS 105.22 (FJ150886) 90 Aureobasidium melanogenumdH 12640 (FJ150889) 97 71 Aureobasidium melanogenum EXF-924 (FJ150883) Aureobasidium melanogenum CBS 105.22 (FJ150886) Aureobasidium leucospermi CBS 130593 (KT693727) 97 Aureobasidium melanogenum EXF-924 (FJ150883) Aureobasidium subglaciale EXF-3640 (FJ150896) Aureobasidium leucospermi CBS 130593 (KT693727)

95 Aureobasidium subglacialedH 13876 (FJ150892) Aureobasidium subglaciale EXF-3640 (FJ150896)

96 Aureobasidium subglaciale EXF-2481 (FJ150895) 95 Aureobasidium subglacialedH 13876 (FJ150892) 96 Selenophoma mahoniae CBS 388.92 (FJ150872) Aureobasidium subglaciale EXF-2481 (FJ150895) Selenophoma mahoniae CBS 388.92 (FJ150872) 100 Aureobasidium iranianum CCTU 268 (KM093738) Aureobasidium iranianum CCTU 268 (KM093738) Aureobasidium sp. SN-2014 (KF758573) 100 Aureobasidium sp. SN-2014 (KF758573) Aureobasidium microstictum CBS 114.64 (FJ150873) Aureobasidium microstictum CBS 114.64 (FJ150873) 54 Aureobasidium caulivorum CBS 242.64 (KT693740) 54 Aureobasidium caulivorum CBS 242.64 (KT693740) Aureobasidium tremulum MCC 1683 (MK503660) Aureobasidium tremulum MCC 1683 (MK503660) 100 Aureobasidium thailandense NRRL 58543 (JX462675) 100 Aureobasidium thailandense NRRL 58543 (JX462675) Aureobasidium thailandense NRRL 58539 (JX462674) Aureobasidium thailandense NRRL 58539 (JX462674) Aureobasidium mangrovei IBRC-M-30266 (KY089087) Aureobasidium mangrovei IBRC-M-30266 (KY089087)

100 Aureobasidium mangrovei IBRC-M-30265 T (KY089085) 100 Aureobasidium mangrovei IBRC-M-30265 T (KY089085) 80 Kabatiella sp. SQU-MA24 (KU945924) 80 Kabatiella sp. SQU-MA24 (KU945924) Sydowia polyspora CBS544.95 (AY152548) Sydowia polyspora CBS544.95 (AY152548)

0.02 0.02 Neighbour-joining tree based on the D1/D2 LSU rDNA region showing Neighbour-joining tree based on the ITS region showing the position of the position of Aureobasidium tremulum sp. nov. among related species Aureobasidium tremulum sp. nov. among related species within genus within genus Aureobasidium. Bootstrap values of above 50 % are given at Aureobasidium. Bootstrap values of above 50 % are given at nodes based nodes based on 1 000 replications. The scale bar represents 2 % sequence on 1 000 replications. The scale bar represents 1 % sequence difference. difference.

Colour illustrations. India, Maharashtra, Aurangabad, Vivekanand Arts, Sardar Dalipsingh Commerce and Science College, Aurangabad. Growth of A. tremulum on potato dextrose agar; light microscopic (LM) view of A. tremu lum; Cryo Scanning Electron Microscopic (CSEM) image of A. tremulum. Scale bars = 5 μm (LM image), 1 μm (CSEM image).

Areeb Inamdar & Nitin N. Adhapure, Department of Biotechnology and Microbiology, Vivekanand Arts, Sardar Dalipsingh Commerce and Science College, Aurangabad 431001, Maharashtra, India; e-mail: [email protected] & [email protected] Rohit Sharma & Mahesh S. Sonawane, National Centre for Microbial Resource (NCMR), National Centre for Cell Science, S.P. Pune University, Ganeshkhind, Pune 411 007, Maharashtra, India; e-mail: [email protected] & [email protected]