DOCSLIB.ORG

Aureobasidium Melanogenum

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Aureobasidium Melanogenum Aureobasidium melanogenum Citation for published version (APA): van Nieuwenhuijzen, E. J., Houbraken, J. A. M. P., Meijer, M., Adan, O. C. G., & Samson, R. A. (2016). Aureobasidium melanogenum: a native of dark biofinishes on oil treated wood. Antonie van Leeuwenhoek, 109(5), 661-683. https://doi.org/10.1007/s10482-016-0668-7 DOI: 10.1007/s10482-016-0668-7 Document status and date: Published: 01/05/2016 Document Version: Publisher’s PDF, also known as Version of Record (includes final page, issue and volume numbers) Please check the document version of this publication: • A submitted manuscript is the version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website. • The final author version and the galley proof are versions of the publication after peer review. • The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal. If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license above, please follow below link for the End User Agreement: www.tue.nl/taverne Take down policy If you believe that this document breaches copyright please contact us at: [email protected] providing details and we will investigate your claim. Download date: 24. Sep. 2021 Antonie van Leeuwenhoek (2016) 109:661–683 DOI 10.1007/s10482-016-0668-7 ORIGINAL PAPER Aureobasidium melanogenum: a native of dark biofinishes on oil treated wood Elke J. van Nieuwenhuijzen . Jos A. M. P. Houbraken . Martin Meijer . Olaf C. G. Adan . Robert A. Samson Received: 7 December 2015 / Accepted: 17 February 2016 / Published online: 27 February 2016 Ó The Author(s) 2016. This article is published with open access at Springerlink.com Abstract The genus Aureobasidium, which is presence of a biofinish and the type of substrate. A. known as a wood staining mould, has been detected melanogenum was detected on wood samples exposed on oil treated woods in the specific stain formation in the Netherlands, Cameroon, South Africa, Australia called biofinish. This biofinish is used to develop a and Norway. ITS-specific PCR amplification, cloning new protective, self-healing and decorative biotreat- and sequencing of DNA extracted from biofinish ment for wood. In order to understand and control samples confirmed results of the culturing based biofinish formation on oil treated wood, the occur- method: A. melanogenum is predominant within the rence of different Aureobasidium species on various Aureobasidium population of biofinishes on pine wood surfaces was studied. Phenotypic variability sapwood treated with raw linseed oil and the outdoor within Aureobasidium strains presented limitations of placement in the Netherlands. morphological identification of Aureobasidium spe- cies. PCR amplification and Sanger sequencing of ITS Keywords Aureobasidium pullulans Á Linseed oil Á and RPB2 were used to identify the culturable Mould staining Á Pine Á Sustainable Á Wood protection Aureobasidium species composition in mould stained wood surfaces with and without a biofinish. The analysed isolates showed that several Aureobasidium species were present and that Aureobasidium melano- Introduction genum was predominantly detected, regardless of the Aureobasidium are wood staining fungi, in particular on wood situated outdoors above the ground (Dickin- Electronic supplementary material The online version of son 1972; Dix and Webster 1995; Bardage 1998; this article (doi:10.1007/s10482-016-0668-7) contains supple- mentary material, which is available to authorized users. Schmidt 2006; Gobakken and Westin 2008). The interest in Aureobasidium has recently increased, E. J. van Nieuwenhuijzen (&) Á J. A. M. P. Houbraken Á because of its role in the formation of biofinishes on M. Meijer Á R. A. Samson wood (Sailer et al. 2010; van Nieuwenhuijzen et al. CBS KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands 2013; van Nieuwenhuijzen et al. 2015; Filippovych e-mail: [email protected] et al. 2015). The term biofinish was introduced for a uniform dark mould covering which emerged outdoors O. C. G. Adan on oil treated wood (van Nieuwenhuijzen et al. 2015). Section Transport in Permeable Media, Department of Applied Physics, University of Technology Eindhoven, Although the protection mechanism and durability of Den Dolech 2, 5600 MB Eindhoven, The Netherlands this biofinish is still under investigation, biofinished 123 662 Antonie van Leeuwenhoek (2016) 109:661–683 wood is considered to be an appealing biocide-free GCPSR multigene phylogenies are required. Next to construction material that has the advantage of also the large subunit and the internal transcribed spacer having self-healing properties. regions (incl. 5.8S rDNA) (ITS) more variable genes Until now it is unknown which Aureobasidium such as translation elongation factor 1a, b-tubulin and species participates in the biofinish formation and RNA polymerase II- second largest subunit (RPB2) whether a biofinish is composed of more than a single have been applied or recommended for phylogenetic species. Although Aureobasidium has been isolated analysis of Aureobasidium species (Zalar et al. 2008; from many organic and inorganic substrates and Manitchotpisit et al. 2009; Peterson et al. 2013; geographical locations (Zalar et al. 2008; Slepecky Gostincˇar et al. 2014). A phylogeny, including all and Starmer 2009; Gaur et al. 2010), the ubiquity of described genera and species within the Aureobasidi- the specific species is unknown. The geographical aceae, is not yet available. location, the combination of wood species and the oil The ITS locus is assigned as the primary barcode treatment may all have an impact on the species for fungal species (Schoch et al. 2012). A large composition of the Aureobasidium population in number of ITS barcode sequences of Aureobasidium biofinishes. Species-specific behaviour, such as phe- species is available in public databases, which makes notype and physiology (Samson et al. 2010; Houbra- this DNA region a suitable marker to identify Aure- ken 2013), should be included in future research in obasidium (Manitchotpisit et al. 2009). To date, no order to understand and control dark mould growth on second fungal barcode has been determined for a oil treated wood. Therefore insight in the species reliable Aureobasidium identification on species level. composition of the biofinish is highly relevant. The aim of this study was to explore the Aureoba- The ascomycete genus Aureobasidium is a member sidium species composition of biofinishes on wood. of the family Aureobasidiaceae within the class of the Culturable Aureobasidium isolates, retrieved from Dothideomycetes (Thambugala et al. 2014; substrates with and without biofinishes, were identi- Wijayawardene et al. 2014). Kabatiella is closely fied. The wood species, oil treatments and exposure related to Aureobasidium based on morphology and sites were related to the culturable species composi- phylogeny (Zalar et al. 2008; Bills et al. 2012; Crous tion. Also direct extraction of biofinish DNA, followed et al. 2011; Peterson et al. 2013; Thambugala et al. by ITS amplification, cloning and sequencing were 2014) and some of these Kabatiella species may used to determine the species compositions of belong to Aureobasidium (Peterson et al. 2013; biofinishes. Thambugala et al. 2014). In addition future studies may result in the transfer of the species Selenophoma mahoniae and Columnosphaeria (Discosphaerina) Materials and methods fagi into Aureobasidium (Yurlova et al. 1999; Peterson et al. 2013; Thambugala et al. 2014). A well-known Substrates and outdoor exposure Aureobasidium species is Aureobasidium pullulans (Zalar et al. 2008; Gostincˇar et al. 2014). The total Nine sample sets were analysed in this study. Each set number of classified Aureobasidium species currently contained oil treated wood samples. Untreated pine varies per database, for example 38 in MycoBank and 13 in GenBank (October 2015). Before DNA sequencing was applied in fungal (oil absent) taxonomy, the species classification system was wood mainly based on physiologic and phenotypic charac- oil impregnaon teristics. In the case of Aureobasidium, colony blocks biofinish outdoor detecon pigmentation was used as a species-specific pheno- exposure & typic characteristic (Zalar et al. 2008; Peterson et al. moisture content sampling 2013). Nowadays, also the genealogical concordance glass sheets phylogenetic species recognition (GCPSR) concept is (oil & wood absent) commonly applied for species delimitation (Taylor Fig. 1 Flowchart illustrating the experimental setup of this et al. 2000). For species delimitation
Load more

Recommended publications
  • University of California Santa Cruz Responding to An
    UNIVERSITY OF CALIFORNIA SANTA CRUZ RESPONDING TO AN EMERGENT PLANT PEST-PATHOGEN COMPLEX ACROSS SOCIAL-ECOLOGICAL SCALES A dissertation submitted in partial satisfaction of the requirements for the degree of DOCTOR OF PHILOSOPHY in ENVIRONMENTAL STUDIES with an emphasis in ECOLOGY AND EVOLUTIONARY BIOLOGY by Shannon Colleen Lynch December 2020 The Dissertation of Shannon Colleen Lynch is approved: Professor Gregory S. Gilbert, chair Professor Stacy M. Philpott Professor Andrew Szasz Professor Ingrid M. Parker Quentin Williams Acting Vice Provost and Dean of Graduate Studies Copyright © by Shannon Colleen Lynch 2020 TABLE OF CONTENTS List of Tables iv List of Figures vii Abstract x Dedication xiii Acknowledgements xiv Chapter 1 – Introduction 1 References 10 Chapter 2 – Host Evolutionary Relationships Explain 12 Tree Mortality Caused by a Generalist Pest– Pathogen Complex References 38 Chapter 3 – Microbiome Variation Across a 66 Phylogeographic Range of Tree Hosts Affected by an Emergent Pest–Pathogen Complex References 110 Chapter 4 – On Collaborative Governance: Building Consensus on 180 Priorities to Manage Invasive Species Through Collective Action References 243 iii LIST OF TABLES Chapter 2 Table I Insect vectors and corresponding fungal pathogens causing 47 Fusarium dieback on tree hosts in California, Israel, and South Africa. Table II Phylogenetic signal for each host type measured by D statistic. 48 Table SI Native range and infested distribution of tree and shrub FD- 49 ISHB host species. Chapter 3 Table I Study site attributes. 124 Table II Mean and median richness of microbiota in wood samples 128 collected from FD-ISHB host trees. Table III Fungal endophyte-Fusarium in vitro interaction outcomes.
    [Show full text]
  • The Evolution of Secondary Metabolism Regulation and Pathways in the Aspergillus Genus
    THE EVOLUTION OF SECONDARY METABOLISM REGULATION AND PATHWAYS IN THE ASPERGILLUS GENUS By Abigail Lind Dissertation Submitted to the Faculty of the Graduate School of Vanderbilt University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in Biomedical Informatics August 11, 2017 Nashville, Tennessee Approved: Antonis Rokas, Ph.D. Tony Capra, Ph.D. Patrick Abbot, Ph.D. Louise Rollins-Smith, Ph.D. Qi Liu, Ph.D. ACKNOWLEDGEMENTS Many people helped and encouraged me during my years working towards this dissertation. First, I want to thank my advisor, Antonis Rokas, for his support for the past five years. His consistent optimism encouraged me to overcome obstacles, and his scientific insight helped me place my work in a broader scientific context. My committee members, Patrick Abbot, Tony Capra, Louise Rollins-Smith, and Qi Liu have also provided support and encouragement. I have been lucky to work with great people in the Rokas lab who helped me develop ideas, suggested new approaches to problems, and provided constant support. In particular, I want to thank Jen Wisecaver for her mentorship, brilliant suggestions on how to visualize and present my work, and for always being available to talk about science. I also want to thank Xiaofan Zhou for always providing a new perspective on solving a problem. Much of my research at Vanderbilt was only possible with the help of great collaborators. I have had the privilege of working with many great labs, and I want to thank Ana Calvo, Nancy Keller, Gustavo Goldman, Fernando Rodrigues, and members of all of their labs for making the research in my dissertation possible.
    [Show full text]
  • Dothistroma Septosporum
    Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and private study only. The thesis may not be reproduced elsewhere without the permission of the Author. Secondary metabolism of the forest pathogen Dothistroma septosporum A thesis presented in the partial fulfilment of the requirements for the degree of Doctor of Philosophy (PhD) in Genetics at Massey University, Manawatu, New Zealand Ibrahim Kutay Ozturk 2016 ABSTRACT Dothistroma septosporum is a fungus causing the disease Dothistroma needle blight (DNB) on more than 80 pine species in 76 countries, and causes serious economic losses. A secondary metabolite (SM) dothistromin, produced by D. septosporum, is a virulence factor required for full disease expression but is not needed for the initial formation of disease lesions. Unlike the majority of fungal SMs whose biosynthetic enzyme genes are arranged in a gene cluster, dothistromin genes are dispersed in a fragmented arrangement. Therefore, it was of interest whether D. septosporum has other SMs that are required in the disease process, as well as having SM genes that are clustered as in other fungi. Genome sequencing of D. septosporum revealed that D. septosporum has 11 SM core genes, which is fewer than in closely related species. In this project, gene cluster analyses around the SM core genes were done to assess if there are intact or other fragmented gene clusters. In addition, one of the core SM genes, DsNps3, that was highly expressed at an early stage of plant infection, was knocked out and the phenotype of this mutant was analysed.
    [Show full text]
  • Virulence Traits and Population Genomics of the Black Yeast Aureobasidium Melanogenum
    Journal of Fungi Article Virulence Traits and Population Genomics of the Black Yeast Aureobasidium melanogenum Anja Cernošaˇ 1,†, Xiaohuan Sun 2,†, Cene Gostinˇcar 1,3,* , Chao Fang 2, Nina Gunde-Cimerman 1,‡ and Zewei Song 2,‡ 1 Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia; [email protected] (A.C.);ˇ [email protected] (N.G.-C.) 2 BGI-Shenzhen, Beishan Industrial Zone, Shenzhen 518083, China; [email protected] (X.S.); [email protected] (C.F.); [email protected] (Z.S.) 3 Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, Qingdao 266555, China * Correspondence: [email protected] or [email protected]; Tel.: +386-1-320-3392 † These authors contributed equally to this work. ‡ These authors contributed equally as senior authors. Abstract: The black yeast-like fungus Aureobasidium melanogenum is an opportunistic human pathogen frequently found indoors. Its traits, potentially linked to pathogenesis, have never been system- atically studied. Here, we examine 49 A. melanogenum strains for growth at 37 ◦C, siderophore production, hemolytic activity, and assimilation of hydrocarbons and human neurotransmitters and report within-species variability. All but one strain grew at 37 ◦C. All strains produced siderophores and showed some hemolytic activity. The largest differences between strains were observed in the assimilation of hydrocarbons and human neurotransmitters. We show for the first time that fungi from the order Dothideales can assimilate aromatic hydrocarbons. To explain the background, we Citation: ˇ Cernoša, A.; Sun, X.; sequenced the genomes of all 49 strains and identified genes putatively involved in siderophore pro- Gostinˇcar, C.; Fang, C.; duction and hemolysis.
    [Show full text]
  • AR TICLE a New Family and Genus in Dothideales for Aureobasidium-Like
    IMA FUNGUS · 8(2): 299–315 (2017) doi:10.5598/imafungus.2017.08.02.05 A new family and genus in Dothideales for Aureobasidium-like species ARTICLE isolated from house dust Zoë Humphries1, Keith A. Seifert1,2, Yuuri Hirooka3, and Cobus M. Visagie1,2,4 1Biodiversity (Mycology), Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON, Canada, K1A 0C6 2Department of Biology, University of Ottawa, 30 Marie-Curie, Ottawa, ON, Canada, K1N 6N5 3Department of Clinical Plant Science, Faculty of Bioscience, Hosei University, 3-7-2 Kajino-cho, Koganei, Tokyo, Japan 4Biosystematics Division, ARC-Plant Health and Protection, P/BagX134, Queenswood 0121, Pretoria, South Africa; corresponding author e-mail: [email protected] Abstract: An international survey of house dust collected from eleven countries using a modified dilution-to-extinction Key words: method yielded 7904 isolates. Of these, six strains morphologically resembled the asexual morphs of Aureobasidium 18S and Hormonema (sexual morphs ?Sydowia), but were phylogenetically distinct. A 28S rDNA phylogeny resolved 28S strains as a distinct clade in Dothideales with families Aureobasidiaceae and Dothideaceae their closest relatives. BenA Further analyses based on the ITS rDNA region, β-tubulin, 28S rDNA, and RNA polymerase II second largest subunit black yeast confirmed the distinct status of this clade and divided strains among two consistent subclades. As a result, we Dothidiomycetes introduce a new genus and two new species as Zalaria alba and Z. obscura, and a new family to accommodate them in ITS Dothideales. Zalaria is a black yeast-like fungus, grows restrictedly and produces conidiogenous cells with holoblastic RPB2 synchronous or percurrent conidiation.
    [Show full text]
  • New Record of Aureobasidium Mangrovei from Plant Debris in the Sultanate of Oman
    CZECH MYCOLOGY 71(2): 219–229, DECEMBER 19, 2019 (ONLINE VERSION, ISSN 1805-1421) New record of Aureobasidium mangrovei from plant debris in the Sultanate of Oman 1 2 1 SARA H. AL-ARAIMI *, ABDULLAH M.S. AL-HATMI ,ABDULKADIR E. ELSHAFIE , 1 3 3 2 SAIF N. AL-BAHRY ,YAHYA M. AL-WAHAIBI ,ALI S. AL-BIMANI ,SYBREN DE HOOG 1 Department of Biology, College of Science, Sultan Qaboos University, P.O. Box 36, Al-Khod, Muscat, P.C. 123, Sultanate of Oman 2 Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands 3 Department of Petroleum and Chemical Engineering, College of Engineering, Sultan Qaboos University, P.O. Box 33, Al-Khod, Muscat, P.C. 123, Sultanate of Oman *corresponding author: [email protected] Al-Araimi S.H., Al-Hatmi A.M.S., Elshafie A.E., Al-Bahry S.N., Al-Wahaibi Y.M., Al- Bimani A.S., de Hoog S. (2019): New record of Aureobasidium mangrovei from plant debris in the Sultanate of Oman. – Czech Mycol. 71(2): 219–229. Aureobasidium mangrovei was isolated from plant debris in Muscat, Sultanate of Oman. The isolate was characterised and compared with related species of this genus for its growth, colony mor- phology, and micromorphology. Molecular analysis of the LSU and ITS rDNA supported final identifi- cation of the isolate. Our record is the second find in the world and the first in the Sultanate of Oman. DNA sequences of the isolated strain showed 99% (ITS) and 100% (LSU) similarity, respectively, with the sequences of the type isolates from Iran, as well as similar growth and colony morphology.
    [Show full text]
  • 비타민나무 열매에서 분리한 Aureobasidium Pullulans의 동정 및 Amylase 활성
    J East Asian Soc Diet Life 225 28(3): 225∼230 (2018) http://dx.doi.org/10.17495/easdl.2018.6.28.3.225 비타민나무 열매에서 분리한 Aureobasidium pullulans의 동정 및 Amylase 활성 이소영1․이희윤2․김계원3․강근옥1† 1국립한경대학교 영양조리과학과, 2경희대학교 식품생명공학과, 3국립한경대학교 산학협력단 Identification of Aureobasidium pullulans from Sea Buckthorn (Hippohae rhamnoides L.) Fruits and Its Amylase Activities So-Young Lee1, Hee-Yoon Lee2, Gye-Won Kim3 and Kun-Og Kang1† 1Dept. of Nutrition and Culinary Science, Hankyong National University, Anseong 17579, Republic of Korea 2Dept. of Food Science and Biotechnology, Kyung Hee University, Seoul 02447, Republic of Korea 3Academic Industry Cooperation, Hankyong National University, Anseong 17579, Republic of Korea ABSTRACT A new yeast strain isolated from Sea Buckthorn (Hippophae rhamnoides L.) fruits was identified and the characteristics of its amylase activity were investigated. A wild yeast strain isolated from Sea Buckthorn (Hippophae rhamnoides L.) fruits was identified as Aureobasidium pullulans using the morphological observations and ITS1-5.8S rRNA-ITS2 PCR. This A. pullulans strain showed the highest amylase activity under the condition of a sodium acetate buffer (pH 5.0) and 60℃. On the other hand, the optimal reaction temperature of the amylase activity of A. pullulans previously isolated from the ocean was 28℃. These results indicate that the enzyme activities of A. pullulans isolated from different sources might not be the same. Unlike previous studies on A. pullulans from the ocean, starch was metabolized more rapidly and the specific activity of amylase was three times higher in the cultures using the distilled water base media compared to the artificial sea water base media.
    [Show full text]
  • Terroir Is the Main Driver of the Epiphytic Bacterial and Fungal Communities of Mango Carposphere in Reunion Island
    fmicb-11-619226 January 13, 2021 Time: 17:16 # 1 ORIGINAL RESEARCH published: 20 January 2021 doi: 10.3389/fmicb.2020.619226 Terroir Is the Main Driver of the Epiphytic Bacterial and Fungal Communities of Mango Carposphere in Reunion Island Ahmed Taîbi1,2, Ronan Rivallan3,4, Véronique Broussolle5, Dominique Pallet2,6, Sylvie Lortal7, Jean-Christophe Meile1,2† and Florentin Constancias2,6*† 1 CIRAD, UMR Qualisud, Saint-Pierre, France, 2 Qualisud, Univ Montpellier, Avignon Université, CIRAD, Institut Agro, Université de La Réunion, Montpellier, France, 3 CIRAD, UMR AGAP, Montpellier, France, 4 AGAP, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France, 5 INRAE, Avignon Université, Sécurité et Qualité des Produits d’Origine Végétale, Avignon, France, 6 CIRAD, UMR Qualisud, Montpellier, France, 7 INRAE, Département Microbiologie et Chaine alimentaire, Edited by: Jouy-en-Josas, France Laurent Dufossé, Université de la Réunion, France The diversity of both bacterial and fungal communities associated with mango surface Reviewed by: Priscila Jane Romano Gonçalves was explored using a metabarcoding approach targeting fungal ITS2 and bacterial 16S Selari, (V3-V4) genomic regions. Fruits were collected in Reunion Island from two different Goiano Federal Institute (IFGOIANO), orchards according to a sampling method which allowed the effect of several pre- Brazil Nuttapon Pombubpa, harvest factors such as geographical location (terroir), cultivars, fruit parts, tree position University of California, Riverside, in the plot, fruit position on the tree (orientation and height), as well as the harvest United States date to be investigated. A total of 4,266,546 fungal and 2,049,919 bacterial reads *Correspondence: Florentin Constancias were recovered then respectively assigned to 3,153 fungal and 24,087 to bacterial fl[email protected] amplicon sequence variants (ASVs).
    [Show full text]
  • Aureobasidium Melanogenum
    Aureobasidium melanogenum Citation for published version (APA): van Nieuwenhuijzen, E. J., Houbraken, J. A. M. P., Meijer, M., Adan, O. C. G., & Samson, R. A. (2016). Aureobasidium melanogenum: a native of dark biofinishes on oil treated wood. Antonie van Leeuwenhoek, 109(5), 661-683. https://doi.org/10.1007/s10482-016-0668-7 DOI: 10.1007/s10482-016-0668-7 Document status and date: Published: 01/05/2016 Document Version: Publisher’s PDF, also known as Version of Record (includes final page, issue and volume numbers) Please check the document version of this publication: • A submitted manuscript is the version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website. • The final author version and the galley proof are versions of the publication after peer review. • The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal.
    [Show full text]
  • Aureobasidium Melanogenum: a Native of Dark Biofinishes on Oil Treated Wood
    Antonie van Leeuwenhoek (2016) 109:661–683 DOI 10.1007/s10482-016-0668-7 ORIGINAL PAPER Aureobasidium melanogenum: a native of dark biofinishes on oil treated wood Elke J. van Nieuwenhuijzen . Jos A. M. P. Houbraken . Martin Meijer . Olaf C. G. Adan . Robert A. Samson Received: 7 December 2015 / Accepted: 17 February 2016 / Published online: 27 February 2016 Ó The Author(s) 2016. This article is published with open access at Springerlink.com Abstract The genus Aureobasidium, which is presence of a biofinish and the type of substrate. A. known as a wood staining mould, has been detected melanogenum was detected on wood samples exposed on oil treated woods in the specific stain formation in the Netherlands, Cameroon, South Africa, Australia called biofinish. This biofinish is used to develop a and Norway. ITS-specific PCR amplification, cloning new protective, self-healing and decorative biotreat- and sequencing of DNA extracted from biofinish ment for wood. In order to understand and control samples confirmed results of the culturing based biofinish formation on oil treated wood, the occur- method: A. melanogenum is predominant within the rence of different Aureobasidium species on various Aureobasidium population of biofinishes on pine wood surfaces was studied. Phenotypic variability sapwood treated with raw linseed oil and the outdoor within Aureobasidium strains presented limitations of placement in the Netherlands. morphological identification of Aureobasidium spe- cies. PCR amplification and Sanger sequencing of ITS Keywords Aureobasidium pullulans Á Linseed oil Á and RPB2 were used to identify the culturable Mould staining Á Pine Á Sustainable Á Wood protection Aureobasidium species composition in mould stained wood surfaces with and without a biofinish.
    [Show full text]
  • The Architecture of Metabolism Maximizes Biosynthetic Diversity in the Largest Class Of
    bioRxiv preprint doi: https://doi.org/10.1101/2020.01.31.928846; this version posted February 1, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Research Article 2 The architecture of metabolism maximizes biosynthetic diversity in the largest class of 3 fungi 4 Authors: 5 Emile Gluck-Thaler, Department of Plant Pathology, The Ohio State University Columbus, OH, USA, 6 and Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA 7 Sajeet Haridas, US Department of Energy Joint Genome Institute, Lawrence Berkeley National 8 Laboratory, Berkeley, CA, USA 9 Manfred Binder, TechBase, R-Tech GmbH, Regensburg, Germany 10 Igor V. Grigoriev, US Department of Energy Joint Genome Institute, Lawrence Berkeley National 11 Laboratory, Berkeley, CA, USA, and Department of Plant and Microbial Biology, University of 12 California, Berkeley, CA 13 Pedro W. Crous, Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The 14 Netherlands 15 Joseph W. Spatafora, Department of Botany and Plant Pathology, Oregon State University, OR, USA 16 Kathryn Bushley, Department of Plant and Microbial Biology, University of Minnesota, MN, USA 17 Jason C. Slot, Department of Plant Pathology, The Ohio State University Columbus, OH, USA 18 corresponding author: [email protected] 19 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.01.31.928846; this version posted February 1, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 19 Abstract: 20 Background - Ecological diversity in fungi is largely defined by metabolic traits, including the 21 ability to produce secondary or "specialized" metabolites (SMs) that mediate interactions with 22 other organisms.
    [Show full text]
  • 207746V2.Full.Pdf
    bioRxiv preprint doi: https://doi.org/10.1101/207746; this version posted November 19, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 1 Genomic features and evolution of the conditionally dispensable 2 chromosome in the tangerine pathotype of Alternaria alternata 3 4 Mingshuang Wang1,2, Huilan Fu1, Xing-Xing Shen2, Ruoxin Ruan3, Nicholas Pun4, Jianping Xu4, 5 Hongye Li1*and Antonis Rokas2* 6 7 1Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China 8 2Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235, USA 9 3Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China 10 4Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada 11 12 *Corresponding authors: Hongye Li and Antonis Rokas 13 E-mail address: [email protected] (H. Li); [email protected] (A. Rokas) bioRxiv preprint doi: https://doi.org/10.1101/207746; this version posted November 19, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 2 14 Abstract 15 The tangerine pathotype of the ascomycete fungus Alternaria alternata is the 16 causal agent of citrus brown spot, which can result in significant losses of both yield 17 and marketability for tangerines and tangerine hybrids worldwide. A conditionally 18 dispensable chromosome (CDC), which harbors the host-selective ACT toxin gene 19 cluster, is required for tangerine pathogenicity of A.
    [Show full text]