US 201001 24746A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2010/0124746A1 Liew (43) Pub. Date: May 20, 2010

(54) METHOD FOR THE DETECTION OF GENE (60) Provisional application No. 60/115,125, filed on Jan. TRANSCRIPTS IN BLOOD AND USES 6, 1999. THEREOF (75) Inventor: Choong-Chin Liew, Toronto (CA) Publication Classification Correspondence Address: (51) 5,/68 (2006.01) EDWARDS ANGELL PALMER & DODGE LLP P.O. BOX SS874 (52) U.S. Cl...... 435/6 BOSTON, MA 02205 (US) (73) Assignee: GeneNews, Inc, (57) ABSTRACT (21) Appl. No.: 12/587,382 The present invention is directed to detection and measure ment of gene transcripts in blood. Specifically provided is a (22) Filed: Oct. 5, 2009 RT-PCR analysis performed on a drop of blood for detecting, O O diagnosing and monitoring diseases using tissue-specific Related U.S. Application Data primers. The present invention also describes methods by (63) Continuation of application No. 10/268,730, filed on which delineation of the sequence and/or quantitation of the Oct. 9, 2002, now Pat. No. 7,598,031, which is a con expression levels of disease-associated genes allows for an tinuation of application No. 09/477,148, filed on Jan. immediate and accurate diagnostic/prognostic test for disease 4, 2000, now abandoned. or to assess the effect of a particular treatment regimen. Patent Application Publication May 20, 2010 Sheet 1 of 7 US 2010/O124746A1

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METHOD FOR THE DETECTION OF GENE and infectious disease in the human body. The process TRANSCRIPTS IN BLOOD AND USES described herein requires a simple blood sample and is, there THEREOF fore, non-invasive compared to conventional practices used to detect tissue specific disease, Such as biopsies. CROSS-REFERENCE TO RELATED 0008. One object of the present invention is to provide a APPLICATION non-invasive method for the diagnosis, prognosis and moni 0001. This application is a continuation of application Ser. toring of genetic and infectious disease in humans and ani No. 10/268,730 filed on Oct. 9, 2002, which is a continuation mals. of U.S. application Ser. No. 09/477,148 filed Jan. 4, 2000, 0009. In one embodiment of the present invention, there is now abandoned, which claims the benefit of U.S. Provisional provided a method for detecting expression of a gene in blood Application No. 60/115,125 filed on Jan. 6, 1999, now aban from a Subject, comprising the steps of: a) quantifying RNA doned. Each of these applications is incorporated herein by from a Subject blood sample; and b) detecting expression of reference in their entirety, including the figures and drawings. the gene in the quantified RNA, wherein the expression of the gene in quantified RNA indicates the expression of the gene in FIELD OF THE INVENTION the subject blood. 0010. In another embodiment of the present invention, 0002 The present invention relates generally to the there is provided a method for detecting expression of one or molecular biology of human diseases. More specifically, the more genes in blood from a Subject, comprising the steps of present invention relates to a process using the genetic infor a) obtaining a subject blood sample; b) extracting RNA from mation contained in human peripheral whole blood for the the blood sample; c) amplifying the RNA; d) generating diagnosis, prognosis and monitoring of genetic and infectious expressed sequence tags (ESTs) from the amplified RNA disease in the human body. product; and e) detecting expression of the genes in the ESTs, wherein the expression of the genes in the ESTs indicates the DESCRIPTION OF THE RELATED ART expression of the genes in the subject blood. Preferably, the 0003. The blood is a vital part of the human circulatory genes are tissue-specific genes. system for the human body. Numerous cell types make up the 0011 Instill another embodiment of the present invention, blood tissue including monocytes, leukocytes, lymphocytes there is provided a method for detecting expression of one or and erythrocytes. Although many blood cell types have been more genes in blood from a Subject, comprising the steps of a) described, there are likely many as yet undiscovered cell obtaining a subject blood sample; b) extracting DNA frag types in the human blood. Some of these undiscovered cells ments from the blood sample; c) amplifying the DNA frag may exist transiently, such as those derived from tissues and ments; and d) detecting expression of the genes in the ampli organs that are constantly interacting with the circulating fied DNA product, wherein the expression of the genes in the blood in health and disease. Thus, the blood can provide an amplified DNA product indicates the expression of the genes immediate picture of what is happening in the human body at in the subject blood. any given time. 0012. In yet another embodiment of the present invention, 0004. The turnover of cells in the hematopoietic system is there is provided a method for monitoring a course of a enormous. It was reported that over one trillion cells, includ therapeutic treatment in an individual, comprising the steps ing 200 billion erythrocytes and 70 billion neutrophilic leu of: a) obtaining a blood sample from the individual; b) kocytes, turn over each day in the human body (Ogawa 1993). extracting RNA from the blood sample; c) amplifying the As a consequence of continuous interactions between the RNA; d) generating expressed sequence tags (ESTs) from the blood and the body, genetic changes that occur within the amplified RNA product; e) detecting expression of genes in cells or tissues of the body will trigger specific changes in the ESTs, wherein the expression of the genes is associated gene expression within blood. It is the goal of the present with the effect of the therapeutic treatment; and f) repeating invention that these genetic alterations be harnessed for diag steps a)-e), wherein the course of the therapeutic treatment is nostic and prognostic purposes, which may lead to the devel monitored by detecting the change of expression of the genes opment of therapeutics for ameliorating disease. in the ESTs. Such a method may also be used for monitoring 0005. The complete profile of gene expression in the cir the onset of overt symptoms of a disease, wherein the expres culating blood remains totally unexplored. It is hypothesized sion of the genes is associated with the onset of the symptoms. that gene expression in the blood is reflective of body state 0013 Instill yet another embodiment of the present inven and, as such, the resultant disruption of homeostasis under tion, there is provided a method for diagnosing a disease in a conditions of disease can be detected through analysis of test Subject, comprising the steps of: a) generating a cDNA transcripts differentially expressed in the blood alone. Thus, library for the disease from a whole blood sample from a the identification of several key transcripts or genetic markers normal Subject; b) generating expressed sequence tag (EST) in blood will provide information about the genetic state of profile from the normal subject clNA library; c) generating a the cells, tissues, organ systems of the human body in health cDNA library for the disease from a whole blood sample from and disease. a test subject; d) generating EST profile from the test subject 0006. The prior art is deficient in non-invasive methods of cDNA library; and e) comparing the test subject EST profile screening for tissue-specific diseases. The present invention to the normal subject EST profile, wherein if the test subject fulfills this long-standing need and desire in the art. EST profile differs from the normal subject EST profile, the test Subject might be diagnosed with the disease. SUMMARY OF THE INVENTION 0014 Instill yet another embodiment of the present inven 0007. This present invention discloses a process of using tion, there is provided a kit for diagnosing, prognosing or the genetic information contained in human peripheral whole predicting a disease, comprising: a) gene-specific primers; blood in the diagnosis, prognosis and monitoring of genetic wherein the primers are designed in Such a way that their US 2010/01 24746 A1 May 20, 2010

sequences contain the opposing ends of two adjacent exons had late onset diabetes type II. FIG. 5C shows standardized for the specific gene with the intron sequence excluded; and levels of insulin gene expressed in each fractionated cell from b) a carrier, wherein the carrier immobilizes the primer(s). whole blood. Such a kit may be applied to a test subject whole blood sample 0024 FIG. 6 shows the differential screening of human to diagnose, prognose or predict a disease. blood cell cDNA library with different cDNA probes of heart 0015. In yet another embodiment of the present invention, and brain tissue. FIG. 6A shows blood cell clNA probes vs. there is provided a kit for diagnosing, prognosing or predict adult heart cDNA probes. FIG. 6B shows blood cell cDNA ing a disease, comprising: a) probes derived from a whole probes vs. human brain cDNA probes. blood sample for a specific disease; and b) a carrier, wherein 0025 FIG. 7 graphically shows the 1,800 unique genes in the carrier immobilizes the probes. Such a kit may be applied human blood and in the human fetal heart grouped into seven to a test Subject whole blood sample to diagnose, prognose or cellular functions. predict a disease. 0016 Furthermore, the present invention provides a DETAILED DESCRIPTION OF THE INVENTION cDNA library specific for a disease, wherein the cDNA library is generated from whole blood samples. 0026. In accordance with the present invention, there may 0017. Other and further aspects, features, and advantages be employed conventional molecular biology, microbiology, of the present invention will be apparent from the following and recombinant DNA techniques within the skill of the art. description of the presently preferred embodiments of the Such techniques are explained fully in the literature. See, e.g., invention. These embodiments are given for the purpose of Sambrook, Fritsch & Maniatis, “Molecular Cloning: A Labo disclosure. ratory Manual (1982): “DNA Cloning: A Practical Approach.” Volumes I and II (D. N. Glovered. 1985): “Oli BRIEF DESCRIPTION OF THE DRAWINGS gonucleotide Synthesis” (M.J. Gaited. 1984); "Nucleic Acid Hybridization B. D. Hames & S. J. Higgins eds. (1985); 0.018 So that the matter in which the above-recited fea “Transcription and Translation'B. D. Hames & S.J. Higgins tures, advantages and objects of the invention, as well as eds. (1984): “Animal Cell Culture R. I. Freshney, ed. others which will become clear, are attained and can be under (1986): “Immobilized Cells And Enzymes' IRL Press, stood in detail, more particular descriptions of the invention (1986); B. Perbal, “A Practical Guide To Molecular Clon briefly summarized above may be had by reference to certain ing' (1984). Therefore, if appearing herein, the following embodiments thereof which are illustrated in the appended terms shall have the definitions set out below. drawings. These drawings form a part of the specification. It (0027. A “cDNA” is defined as copy-DNA or complemen is to be noted, however, that the appended drawings illustrate tary-DNA, and is a product of a reverse transcription reaction preferred embodiments of the invention and therefore are not from an mRNA transcript. “RT-PCR refers to reverse tran to be considered limiting in their scope. Scription polymerase chain reaction and results in production 0019 FIG. 1 shows the following RNA samples prepared of cDNAs that are complementary to the mRNA template(s). from human blood; FIG. 1A: Lane 1, Molecular weight 0028. The term "oligonucleotide' is defined as a molecule marker; Lane 2, RT-PCR on APP gene; Lane 3, PCR on APP comprised of two or more deoxyribonucleotides, preferably gene; Lane 4, RT-PCR on APC gene; Lane 5, PCR on APC more than three. Its exact size will depend upon many factors gene: FIG. 1B: Lanes 1 and 2, RT-PCR and PCR of BMyHC, which, in turn, depend upon the ultimate function and use of respectively; Lanes 3 and 4, RT-PCR of BMyHC from RNA the oligonucleotide. The term “primer' as used herein refers prepared from human fetal and human adult heart, respec to an oligonucleotide, whether occurring naturally as in a tively; Lane 5, Molecular weight marker. purified restriction digest or produced synthetically, which is 0020 FIG. 2 shows quantitative RT-PCR analysis per capable of acting as a point of initiation of synthesis when formed on RNA samples extracted from a drop of blood. placed under conditions in which synthesis of a primer exten Forward primer (5'-GCCCTCTGGGGACCTGAC-3', SEQ sion product, which is complementary to a nucleic acid ID No. 1) of exon 1 and reverse primer (5'-CCCACCTG Strand, is induced, i.e., in the presence of nucleotides and an CAGGTCCTCT-3", SEQ ID No. 2) of exons 1 and 2 of inducing agent Such as a DNA polymerase and at a Suitable insulin gene. Blood samples of 4 normal Subjects were temperature and pH. The primer may be either single assayed. Lanes 1, 3, 5 and 7 represent overnight “fasting stranded or double-stranded and must be sufficiently long to blood sample and lanes 2, 4, 6 and 8 represent “non-fasting prime the synthesis of the desired extension product in the samples. presence of the inducing agent. The exact length of the primer 0021 FIG. 3 shows quantitative RT-PCR analysis per will depend upon many factors, including temperature, formed on RNA samples extracted from a drop of blood. source of primer and the method used. For example, for Lanes 1 and 2 represent normal healthy person and lane 3 diagnostic applications, depending on the complexity of the represents late-onset diabetes (Type II) and lane 4 represents target sequence, the oligonucleotide primer typically con asymptomatic diabetes. tains 15-25 or more nucleotides, although it may contain 0022 FIG. 4 shows multiple RT-PCR assay in a drop of fewer nucleotides. The factors involved in determining the blood. Primers were derived from insulin gene (INS), zinc appropriate length of primer are readily known to one of finger protein gene (ZFP) and house-keeping gene (GADH). ordinary skill in the art. Lane 1 represents normal person. Lane 2 represents late-onset 0029. As used herein, random sequence primers refer to a diabetes and lane 3 represents asymptomatic diabetes. composition of primers of random sequence, i.e. not directed 0023 FIG. 5 shows standardized levels of insulin gene towards a specific sequence. These sequences possess Suffi (FIG. 5A) and ZFP gene (FIG. 5B) expressed in a drop of cient complementary to hybridize with a polynucleotide and blood. The first three subjects were normal, second two sub the primer sequence need not reflect the exact sequence of the jects showed normal glucose tolerance, and the last Subject template. US 2010/01 24746 A1 May 20, 2010

0030) “Restriction fragment length polymorphism” refers animal. The genes are non-cancer-associated and tissue-spe to variations in DNA sequence detected by variations in the cific genes. Still preferably, the amplification is performed by length of DNA fragments generated by restriction endonu RT-PCR using random sequence primers or gene-specific clease digestion. primers. 0031. A standard Northern blotassay can be used to ascer 0035. In still another embodiment of the present invention, tain the relative amounts of mRNA in a cellor tissue obtained there is provided a method for detecting expression of one or from plant or other tissue, in accordance with conventional more genes in blood from a Subject, comprising the steps of Northern hybridization techniques known to those persons of a) obtaining a Subject blood sample; b) extracting DNA frag ordinary skill in the art. The Northern blot uses a hybridiza ments from the blood sample; c) amplifying the DNA frag tion probe, e.g. radiolabelled cDNA, either containing the ments; and d) detecting expression of the genes in the ampli full-length, single stranded DNA or a fragment of that DNA fied DNA product, wherein the expression of the genes in the sequence at least 20 (preferably at least 30, more preferably at amplified DNA product indicates the expression of the genes least 50, and most preferably at least 100 consecutive nucle in the subject blood. otides in length). The DNA hybridization probe can be 0036. In yet another embodiment of the present invention, labelled by any of the many different methods known to those there is provided a method for monitoring a course of a skilled in this art. The labels most commonly employed for therapeutic treatment in an individual, comprising the steps these studies are radioactive elements, enzymes, chemicals of: a) obtaining a blood sample from the individual; b) which fluoresce when exposed to ultraviolet light, and others. extracting RNA from the blood sample; c) amplifying the A number of fluorescent materials are known and can be RNA; d) generating expressed sequence tags (ESTs) from the utilized as labels. These include, for example, fluorescein, amplified RNA product; e) detecting expression of genes in rhodamine, auramine, Texas Red, AMCA blue and Lucifer the ESTs, wherein the expression of the genes is associated Yellow. A particular detecting material is anti-rabbitantibody with the effect of the therapeutic treatment; and f) repeating prepared in goats and conjugated with fluorescein through an steps a)-e), wherein the course of the therapeutic treatment is isothiocyanate. Proteins can also be labeled with a radioactive monitored by detecting the change of expression of the genes element or with an enzyme. The radioactive label can be in the ESTs. Such a method may also be used for monitoring detected by any of the currently available counting proce the onset of overt symptoms of a disease, wherein the expres dures. The preferred isotope may be selected from H, C, sion of the genes is associated with the onset of the symptoms. 32P 35S, 36C1, 5 Cr, 57Co, 58Co, 5°Fe, 90Y. 125I, 13 II, and Preferably, the amplification is performed by RT-PCR, and Re. Enzyme labels are likewise useful, and can be detected the change of the expression of the genes in the ESTs is by any of the presently utilized colorimetric, spectrophoto monitored by sequencing the ESTs and comparing the result metric, fluorospectrophotometric, amperometric or gasomet ing sequences at various time points; or by performing single ric techniques. The enzyme is conjugated to the selected nucleotide polymorphism analysis and detecting the variation particle by reaction with bridging molecules such as carbo of a single nucleotide in the ESTs at various time points. diimides, diisocyanates, glutaraldehyde and the like. Many 0037. In still yet another embodiment of the present inven enzymes which can be used in these procedures are known tion, there is provided a method for diagnosing a disease in a and can be utilized. The preferred are peroxidase, B-glucu test Subject, comprising the steps of: a) generating a cDNA ronidase, B-D-glucosidase, B-D-galactosidase, urease, glu library for the disease from a whole blood sample from a cose oxidase plus peroxidase and alkaline phosphatase. U.S. normal Subject; b) generating expressed sequence tag (EST) Pat. Nos. 3,654,090, 3,850,752, and 4,016,043 are referred to profile from the normal subject clNA library; c) generating a by way of example for their disclosure of alternate labeling cDNA library for the disease from a whole blood sample from material and methods. a test subject; d) generating EST profile from the test subject 0032. As used herein, “individual' refers to human sub cDNA library; and e) comparing the test subject EST profile jects as well as non-human Subjects. The examples herein are to the normal subject EST profile, wherein if the test subject not meant to limit the methodology of the present invention to EST profile differs from the normal subject EST profile, the human Subjects only, as the instant methodology is useful in test Subject might be diagnosed with the disease. the fields of Veterinary medicine, animal Sciences and such. 0038. In still yet another embodiment of the present inven 0033. In one embodiment of the present invention, there is tion, there is provided a kit for diagnosing, prognosing or provided a method for detecting expression of a gene in blood predicting a disease, comprising: a) gene-specific primers; from a subject, comprising the steps of: a) quantifying RNA wherein the primers are designed in Such a way that their from a subject blood sample; and b) detecting expression of sequences contain the opposing ends of two adjacent exons the gene in the quantified RNA, wherein the expression of the for the specific gene with the intron sequence excluded; and gene in quantified RNA indicates the expression of the gene in b) a carrier, wherein the carrier immobilizes the primer(s). the Subject blood. An example of the quantifying method is by Preferably, the gene-specific primers are selected from the mass spectrometry. group consisting of insulin-specific primers, atrial natriuretic 0034. In another embodiment of the present invention, factor-specific primers, Zinc finger protein gene-specific there is provided a method for detecting expression of one or primers, beta-myosin heavy chain gene-specific primers, more genes in blood from a Subject, comprising the steps of amyloid precursor protein gene-specific primers, and a) obtaining a subject blood sample; b) extracting RNA from adenomatous polyposis-coli protein gene-specific primers. the blood sample; c) amplifying the RNA; d) generating Further preferably, the gene-specific primers are selected expressed sequence tags (ESTs) from the amplified RNA from the group consisting of SEQID Nos. 1 and 2; and SEQ product; and e) detecting expression of the genes in the ESTs, ID Nos. 5 and 6. Such a kit may be applied to a test subject wherein the expression of the genes in the ESTs indicates the whole blood sample to diagnose, prognose or predict a dis expression of the genes in the subject blood. Preferably, the ease by detecting the quantitative expression levels of specific Subject is a fetus, an embryo, a child, an adult or a non-human genes associated with the disease in the test Subject and then US 2010/01 24746 A1 May 20, 2010 comparing to the levels of same genes expressed in a normal beta-myosin heavy chain gene (BMyHC), amyloid precursor Subject. Such a kit may also be used for monitoring a course protein (APP) gene and adenomatous polyposis-coli protein of therapeutic treatment or monitoring the onset of overt (APC) gene. The PCR products were also subjected to auto symptoms of a disease. mated DNA sequencing to Verify the sequences as derived 0039. In yet another embodiment of the present invention, from the specific transcripts of blood. there is provided a kit for diagnosing, prognosing or predict ing a disease, comprising: a) probes derived from a whole Example 5 blood sample for a specific disease; and b) a carrier, wherein the carrier immobilizes the probes. Such a kit may be applied Detection of Tissue Specific Gene Expression in to a test Subject whole blood sample to diagnose, prognose or Human Blood Using RT-PCR predict a disease by detecting the quantitative expression levels of specific genes associated with the disease in the test 0046. The beta-myosin heavy chain gene (BMyHC) tran Subject and then comparing to the levels of same genes script (mRNA) is known to be highly expressed in ventricles expressed in a normal Subject. Such a kit may also be used for of the human heart. This sarcomeric protein is important for monitoring a course of therapeutic treatment or monitoring heart muscle contraction and its presence would not be the onset of overt symptoms of a disease. expected in other non-muscle tissues and blood. In 1990, the 0040. Furthermore, the present invention provides a gene for human cardiac BMyHC was completely sequenced cDNA library specific for a disease, wherein the cDNA (Liew et al. 1990) and was comprised of 41 exons and 42 library is generated from whole blood samples. introns. 0041. The following examples are given for the purpose of 0047. The method of reverse transcription polymerase illustrating various embodiments of the invention and are not chain reaction (RT-PCR) was used to determine whether this meant to limit the present invention in any fashion. cardiac specific mRNA is also present in human blood. A pair of primers was designed; the forward primer (SEQID No. 3) Example 1 was on the boundary of exons 21 and 22, and the reverse primer (SEQID No. 4) was on the boundary of exons 24 and Construction of a cDNA Library 25. This region of mRNA is only present in BMyHC and is not 0042 RNA extracted from human tissues (including fetal found in the alpha-myosin heavy chain gene (CMyHC). heart, adult heart, liver, brain, prostate gland and whole 0048. A blood sample was first treated with lysing buffer blood) were used to construct unidirectional cDNA libraries. and then undergone centrifuge. The resulting pellets were The first mammalian heart clNA library was constructed as further processed with RT-PCR. RT-PCR was performed early as 1982. Since then, the methodology has been revised using the total blood cell RNA as a template. A nested PCR and optimal conditions have been developed for construction product was generated and used for sequencing. The sequenc of human heart and hematopoietic progenitor cDNA libraries ing results were subjected to BLAST and the identity of exons (Liew et al., 1984; Liew 1993, Claudio et al., 1998). Most of 21 to 25 was confirmed to be from BMyHC (FIG. 1A). the novel genes which were identified by sequence annotation 0049. Using the same method just described, two other can now be obtained as full length transcripts. tissue specific genes—amyloid precursor protein (APP for ward primer, SEQID No. 7: reverse primer, SEQID No. 8) Example 2 found in the brain and associated with Alzheimer's disease, Catalogue of Blood Cell ESTs and adenomatous polyposis coli protein (APC) found in the colon and rectum and associated with colorectal cancer 0.043 Random partial sequencing of expressed sequence (Groden et al. 1991; Santoro and Groden 1997)—were also tags (ESTs) of cDNA clones from the blood cell library was detected in the RNA extracted from human blood (FIG. 1B). carried out to establish an EST database of blood. The known genes as derived from the ESTs were categorized into seven Example 6 major cellular functions (Hwang, Dempsey et al., 1997). Multiple RT-PCR Analysis on a Drop of Blood from Example 3 a Normal/Diseased Individual Differential Screening of cDNA Library 0050. A drop of blood was extracted to obtain RNA to 0044 cDNA probes generated from transcripts of each carry out quantitative RT-PCR analysis. Specific primers for tissue were used to hybridize the blood cell cDNA clones the insulin gene were designed: forward primer (5'-GC (Liew et al., 1997). The “positive' signals which were hybrid CCTCTGGGGACCTGAC-3', SEQID No. 1) of exon 1 and ized with P-labelled cDNA probes were defined as genes reverse primer (5'-CCCACCTGCAGGTCCTCT-3", SEQID which shared identity with blood and respective tissues. The No. 2) of exons 1 and 2 of insulin gene. Such reverse primer “negative spots which were not exposed to P-labelled cDNA was obtained by deleting the intron between the exons 1 and probes were considered to be blood-cell-enriched or low fre 2. Blood samples of 4 normal Subjects were assayed. It was quency transcripts. found that the insulin gene is expressed in the blood and the quantitative expression of the insulin gene in a drop of blood Example 4 is influenced by fasting and non-fasting states of normal healthy subjects (FIG. 2). This very low level of expression of Reverse Transcriptase-Polymerase Chain Reaction the insulin gene reflects the phenotypic status of a person and (RT-PCR) Assay strongly suggests that there is a physiological and pathologi 0045 RNA extracted from samples of human tissue was cal role for its expression, contrary to the basal or illegitimate used for RT-PCR analysis (Jin et al. 1990). Three pairs of theory of transcription suggested by Chelly et al. (1989) and forward and reverse primers were designed for human cardiac Kimoto (1998). US 2010/01 24746 A1 May 20, 2010

0051 Same quantitative RT-PCR analysis was performed together with the seven cellular functions related to percent using insulin specific primers on RNA samples extracted distribution of known genes in blood and in the fetal heart. from a drop of blood from a normal healthy person, a person 0058. From 20,000 ESTs, 1,800 have been identified as having late-onset diabetes (Type II) and a person having known genes which may not all appear in the hemopoietic asymptomatic diabetes. It was found that the insulin gene is system. For example, the insulin gene and the atrial natri expressed differentially amongst Subjects that are healthy, uretic factor gene have not been detected in these 20,000 diagnosed as type II diabetic, and also in an asymptomatic ESTs but their transcripts were detected in a drop of blood, preclinical patient (FIG. 3). strongly suggesting that all transcripts of the human genome 0052 Similarly, specific primers for the atrial natriuretic can be detected by performing RT-PCR analysis on a drop of factor (ANF) gene were designed (forward primer, SEQ ID blood. No. 5; reverse primer, SEQID No. 6) and RT-PCR analysis 0059. In addition, approximately 400 novel genes have was performed on a drop of blood. ANF is known to be highly been identified from the 20,000 ESTs characterized to date, expressed in heart tissue biopsies and in the plasma of heart and these will be subjected to full length sequencing and open failure patients. However, atrial natriuretic factor was reading frame alignment to reduce the actual number of novel observed to be expressed in the blood and the expression of ESTs prior to screening for disease markers. the atrial natriuretic factor gene is significantly higher in the 0060 Analysis of the approximately 6,283 ESTs which blood of patients with heart failure as compared to the blood have known matches in the GenBank databases revealed that of a normal control patient. this dataset represents over 1,800 unique genes. These genes 0053 Specific primers for the zinc finger protein gene have been catalogued into seven cellular functions. Compari (ZFP forward primer, SEQID No. 9; reverse primer, SEQID sons of this set of unique genes with ESTs derived from No. 10) were also designed and RT-PCR analysis was per human brain, heart, lung and kidney demonstrated a greater formed on a drop of blood. ZFP is known to be high in heart than 50% overlap in expression (Table 1). tissue biopsies of cardiac hypertrophy and heart failure patients. In the present study, the expression of ZFP was observed in the blood as well as differential expression levels TABLE 1 of ZFP amongst the normal, diabetic and asymptomatic pre Overlap of Genes Expressed in Blood clinical subjects (FIG. 4); although neither of the non-normal Subjects has been specifically diagnosed as Suffering from Tissues ESTs 8:8 Overlap in Blood cardiac hypertrophy and/or heart failure, the higher expres brain 134,000 60% sion levels of the ZFP gene in their blood may indicate that heart 65,000 59% these subjects are headed in that general direction. lung 60,200 S8% 0054. It was hypothesized that a housekeeping gene such kidney 32,300 S4% as glyceraldehyde dehydrogenase (GADH) which is required *Estimated from limited known genes of about 1,800 as derived from the and highly expressed in all cells would not be differentially database of 6.297 ESTs from human blood cell library. *Obtained from the National Centre of Biotechnology Information (NCBI), expressed in the blood of normal vs. disease subjects. This U.S.A. hypothesis was confirmed by RT-PCR using GADH specific primers (FIG. 4). Thus, GADH is useful as an internal control. 0055 Standardized levels of insulin gene or ZFP gene Example 8 expressed in a drop of blood were estimated using a house keeping gene as an internal control relative to insulin or ZFP Blood Cell ESTS expressed (FIGS. 5A & 5B). The levels of insulin gene expressed in each fractionated cell from whole blood were 0061 The results from the differential screening clearly also standardized and shown in FIG. 5C. indicate that the transcripts expressed in the whole blood are reflective of genes expressed in all cells and tissues of the Example 7 body. More than 95% of detectable spots were identical from two different tissues. The remaining 5% of spots may repre Human Blood Cell cDNA Library sent cell- or tissue-specific transcripts; however, results obtained from partial sequencing to generate ESTs of these 0056. In order to further substantiate the present invention, clones revealed most of them not to be cell- or tissue-specific differential screening of the human blood cell cDNA library transcripts. Therefore, the negative spots are postulated to be was conducted. cDNA probes derived from human blood, reflective of low abundance transcripts in the tissue from adult heart or brain were respectively hybridized to the human which the cDNA probes were derived. blood cDNA library clones. As shown in FIG. 7, more than 0062 An alternative approach that was employed to iden 95% of the “positively' identified clones are identical tify transcripts expressed at low levels is the large-scale gen between the blood and other tissue samples. eration of expressed sequence tags (ESTs). There is substan 0057 DNA sequencing of randomly selected clones from tial evidence regarding the efficiency of this technology to the human whole blood cell cloNA library was also per detect previously characterized (known) and uncharacterized formed. This allowed information regarding the cellular func (unknown or novel) genes expressed in the cardiovascular tion of blood to be obtained concurrently with gene identifi system (Hwang & Dempsey et al. 1997). In the present inven cation. More than 20,000 expressed sequence tags (ESTs) tion, 20,000 ESTs have been produced from a human blood have been generated and characterized to date, 17.6% of cell cDNA library and resulted in the identification of which did not result in a statistically significant match to approximately 1,800 unique known genes (Table 2) entries in the GenBank databases and thus were designated as 0063. In the most recent GenBank release, analysis of “Novel ESTs. These results are Summarized in FIG. 7 more than 300,000 ESTs in the database (dbESTs) generated US 2010/01 24746 A1 May 20, 2010

more than 48,000 gene clusters which are thought to represent 0064. Most recently, a cDNA library of human hemato approximately 50% of the genes in the human genome. Only poietic progenitor stem cells has also been constructed. From 4,800 of the dbPSTs are blood-derived. In the present inven the limited set of 1,000 ESTs, there are at least 200 known tion, 20,000 ESTs have been obtained to date from a human genes that are shared with other tissue related genes (Claudio blood cDNA library, which provides the world's most infor et al. 1998). mative database with respect to blood cell transcripts. From 0065 Table 2 demonstrates the expression of known genes the limited amount of information generated so far (i.e. 1,800 of specific tissues in blood cells. Previously, only the presence unique genes), it has already been determined that more than of “housekeeping genes would have been expected. Addi 50% of the transcripts are found in other cells or tissues of the tionally, the presence of at least 25 of the currently known 500 human body (Table 2). Thus, it is expected that by increasing genes corresponding to molecular drug targets was detected. the number of ESTs generated, more genes will be identified These molecular drug targets are used in the treatment of a that have an overlap in expression between the blood and variety of diseases which involve inflammation, renal and other tissues. Furthermore, the transcripts for several genes cardiovascular function, neoplastic disease, immunomodula which are known to have tissue-restricted patterns of expres tion and viral infection (Drews & Ryser, 1997). It is expected sion (i.e. BMyHC, APP. APC, ANF, ZFP) have also been that additional novel ESTs will represent future molecular demonstrated to be present in blood. drug targets.

TABLE 2 Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

100 kDa coactivator 2 U22055 -- -- 10 kD protein (BC10) 2 AFOS3470 ------14-3-3 epsilon 2 U54778 ------14-3-3 protein 11 U28964 ------15 kDa selenoprotein AFOS1894 ------(SEP15) 1-phosphatidylinositol-4- S78798 phosphate 5-kinase isoform C 23 kD highly basic protein 21 XS 6932 ------2-5A-dependent RNase L10381 2'-5'oligoadenylate 4 M87284 B synthetase 2 (OAS2) 26S proteasome subunit 11 AFO86708 36 kDa phosphothyrosine 2 A.22328O T -- protein 3-7 gene product (non- D64159 exact 86% aa) 3-phosphoglycerate AFOO6043 T ------dehydrogenase (PGAD) 3-prime-phosphoadenosine 2 US3447 ------5-prime-phosphosulfate synthase 1 (PAPSS1) 46 kd mannose 6- 1 X56257 phosphate receptor (MPR46) (low match) 5-aminoimidazole-4- 1 D89976 carboxamide ribonucleotide transformylase 5'-nucleotidase D38524 T -- -- 6-phosphofructo-2- 1 D498.18 -- kinase? fructose-2,6- biphosphatase 4 (PFKFB4) 6-phosphofructo-2- 1 AFO41829 kinase? fructose-2,6- bisphosphatase (PF2K) 71 kd heat shock cognate 23 YOO371 protein hsc70 76 kDa membrane protein 2 ------(P76) 8-oxoguanine DNA 1 U96710 B -- -- glycosylase (OGG1) a disintegrin and 1 AFOO961S T -- metalloprotease domain 10 (ADAM10) US 2010/01 24746 A1 May 20, 2010

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu a disintegrin and 1 D265.79 metalloprotease domain 8 (ADAM8) A kinase anchor protein 95 Y11997 B, T -- -- (AKAP95) activated Akinase anchor protein, X97335 ------149 kD (AKAP149) A4 differentiation U93305 dependent protein (A4), triple LIM domain protein (LMO6), and synaptophysin (SYP); calcium channel alpha-1 subunit (CACNA1F) ABL and putative M8604 UO7561 Met protein Absent in melanoma 1 U83115 -- (AIM1) accessory proteins Z31696 -- BAP31 BAP29 (DXS1357E) acetyl-Coenzyme A X12966 acyltransferase (peroxisomal 3-oxoacyl Coenzyme A thiolase) (ACAA) acetyl-Coenzyme A T lymphoma -- transporter (ACATN) acidic 82 kDa protein U15552 acidic protein rich in YO7969 ------leucines (SSP29) Aconitase 2, mitochondrial ------(ACO2) actin binding protein AFOS9569 MAYVEN actin, beta (ACTB) 158 XO4098 T, B -- -- actin, beta (ACTB) (non M10277 exact, low match 73%) actin, gamma (low score) KOO791 actin, gamma 1 (ACTG 1) XO4098 ------high in many libraries actin-binding LIM protein D31883 : ------(ABLIM) Actinin, alpha 1 (ACTN1) M95178 ------actinin, alpha 4 (ACTN4) 1 D8998O -- -- activated p21cdc42Hs L13738 -- kinase (ACK) activated RNA polymerase X798.05 ------II transcription cofactor 4 (PC4) activating transcription XSSS44 -- factor 1 (ATF1) activating transcription X15875 -- -- factor 2 (ATF2) activating transcription M86842 -- -- factor 4 (tax-responsive enhancer element B67) (ATF4) active BCR-related gene UO1147 ------(ABR) acyl-CoA oxidase (AOX) 1 acyl-Coenzyme A M16827 dehydrogenase, C-4 to C 12 straight chain (ACADM) acyl-Coenzyme A D43682 dehydrogenase, very long chain (ACADVL) acyloxyacylhydrolase M62840 (neutrophil) (AOAH) US 2010/01 24746 A1 May 20, 2010

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu adaptin, delta (ADTD) 2 U91930 -- -- adaptin, delta (ADTD) (non 1 ACOOS328 exact 59%) adaptin, gamma (ADTG) 1 Y12226 ------adaptor complex sigma3B 2 X99459 : -- -- (AP3S3) 8. aptor protein p150 YO8991 adducin 1 (alpha) (ADD1) LO7261 -- -- adducin 1 (alpha) (add1) L29296 ------adducin 3 (gamma) (ADD3) U37122 B, W ------adenine nucleotide MS7424 -- -- anslocator 2 (fibroblast) (ANT2) adenine nucleotide O2683 translocator 2 (fibroblast) (ANT2) (non-exact 81%) adenine nucleotide O2683 translocator 2 (fibroblast) (ANT2) (non-exact, 79%) adenine nucleotide O2683 translocator 2 (fibroblast) (ANT2) (non-exact, 86%) adenine nucleotide O3592 ------translocator 3 (liver) (ANT3) adenosine deaminase, U18121 -- -- RNA-specific (ADAR) adenylate cyclase 3 AFO33861 (ADCY3) adenylate cyclase 7 D25538 (ADCY7) adenylate kinase 2 (AK2) U399.45 ------adenylate kinase 3 (AK3) X60673 (non-exact, 67%) adenylyl cyclase 28 M98474 T -- -- associated protein (CAP) adipose differentiation X97324 ------related protein; adipophilin (ADFP) ADP-ribosylation factor 1 13 M84326 ------(ARF1) ADP-ribosylation factor 3 M33384 -- -- (ARF3) ADP-ribosylation factor 4 M36341 T lymphoma -- -- (ARF4) ADP-ribosylation factor 5 M57567 (ARF5) ADP-ribosylation factor domain protein 1, 64 kD (ARFD1) ADP-ribosyltransferase M32721 -- (NAD+: poly (ADP-ribose) polymerase) (ADPRT) adrenergic, beta, receptor X61157 B kinase 1 (ADRBK1) adrenoleukodystrophy-like AJOOO327 1 (ALDL1) AE-binding protein 1 D864.79 (AEBP1) (non-exact, 62%) AF-17 A-gamma-globin WOOS14 A-gamma-globin JOO176 (chromosome 11 allele) agammaglobulinaemia tyrosine kinase (ATK) AHNAKnucleoprotein M8O899 -- (desmoyokin) (AHNAK) US 2010/01 24746 A1 May 20, 2010

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu alanyl (membrane) 1 X13276 -- -- aminopeptidase (aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150) (ANPEP) 8. cohol dehydrogenase 5 1 M298.72 (class III), chi polypeptide (ADH5) 8. ehyde dehydrogenase 1 AFOO3341 -- -- , soluble (ALDH1) ehyde dehydrogenase 2 UT5286 O (fatty aldehyde dehydrogenase) (ALDH10) ehyde reductase 1 (low 3 JO4795 B ------Km aldose reductase) (ALDR1) aldo-keto reductase family 2 JO4794 B -- -- , member A1 (aldehyde reductase) (AKR1A1) O-keto reductase family 1 D17793 ------, member C3 (3-alpha ydroxysteroid e hydrogenase, type II) (A KR1C3) O-keto reductase family 1 Y16675 ------, member A2 (aflatoxin ehyde reductase) s KR7A2) olase A, fructose 7 X12447 -- -- sphosphate (ALDOA) olase C, fructose 2 XOS196 -- -- isphosphate (ALDOC) kaline phosphatase, 1 4502O62 verbone/kidney (ALPL) LL-1 (=L04731; L04284 4 Z6978O RX) pha mannosidase II 1 DSS649 -- eOzyme pha thalassemiamental 3 UT5653 ------re ardation syndrome X linked (ATRX) 8. pha-2 macroglobulin 1 Z11711 8. pha-2-globin 2 VOOS16 8. pha-2-macroglobulin 1 UO6985 receptor lipoprotein eceptor protein A2MR/LRP) pha-polypeptide of N 1 M13520 cetyl-alpha lucosaminidase (HEXA) pha-spectrin X86901 8. pha-subunit of Gi2 a XO7854 (GTP-binding signal transduction protein) aminin receptor 1 (67kD); 2 JO3799 T ------Ribosomal protein SA (LAMR1) aminolevulinate, delta-, 1 X64467 dehydratase (ALAD) amino-terminal enhancer of 2 X73358 ------split (AES) amino-terminal enhancer of 3 UO4241 B ------split (AES) AMP deaminase isoform L 8 M91029 (AMPD2) US 2010/01 24746 A1 May 20, 2010 10

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu amphiphysin (Stiff-Mann 1 UO7616 B -- syndrome with breast cancer 128 kD autoantigen) (AMPH) amphiphysin (Stiff-Mann 1 UO7616 syndrome with breast cancer 128 kD autoantigen) (AMPH) (non-exact, 68%) amphiphysin (Stiff-Mann 1 UO7616 syndrome with breast cancer 128 kD autoantigen) (AMPH) (non-exact, 68%) amphiphysin II 4 U87558 -- -- amphiphysin II (67% aa 1 AFO6891S amphiphysin?) amphiphysin II (non-exact 1 AFOO1383 69% aa) amphiphysin-like (AMPHL) 1 U684-85 -- amphiphysin-like (AMPHL) 1 AFO68918 (low match) AMY1 1 DSO692 B, T -- amyloid beta (A4) 1 L77864 ------precursor protein-binding, family B, member 1 (Fe(55) (APBB1) amyloid beta (A4) 6 L27631 T lymphoma ------precursor-like protein 2 (APLP2) ankyrin 3, node of Ranvier 1 U4396S (ankyrin G) (ANK) (non exact, 50%) annexin I (lipocortin I) 1 XOS908 ------(ANX1) annexin II 1 D28.364 annexin II (lipocortin II; DOOO17 -- high in many libraries calpactin I, heavy polypeptide) (ANX2) annexin IV (placental 1 M19383 anticoagulant protein II) (ANX4) annexin V (endonexin II) 2 M21731 ------(ANX5) annexin V (endonexin II) 1 M19384 ------(ANXV) annexin VI (p68) (ANX6) 6 YOOO97 ------annexin VII (synexin) 1 JO4S43 : ------(ANX7) antigen identified by 2 M16279 ------monoclonal antibodies 12E7, F21 and O13 (MIC2) antigen identified by 3 JO2939 monoclonal antibodies 4F2, TRA1.10, TROP4, and T43 (MDU1) antigen TQ1 1 anti-oxidant protein 2 (non 1 D14662 Selenium glutathione peroxidase, acidic calcium independent phospholipase A2) (KIAAO106) APEX nuclease S X66133 (multifunctional DNA repair enzyme) (APEX) Apollipoprotein L (APOL) 1 Z82215 (59% aa) apoptosis inhibitor 1 (API1) 1 L49431 -- -- apoptosis inhibitor 4 1 UT5285 B, W : : : (survivin) (API4) US 2010/01 24746 A1 May 20, 2010 11

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu apoptosis inhibitor 5 (API5) 1 U83857 T lymphoma -- apoptosis specific protein 1 Y11588 B : -- (ASP) apoptotic protease 1 AFO13263 B activating factor (APAF1) aquaporin 3 (AQP3) 1 ABOO1325 T aquaporin 9 (AQP9) 7 AB008775 Tactivated : arachidonate 12 1 MS8704 T lipoxygenase (ALOX12) arachidonate 5 3 X52195 -- lipoxygenase-activating protein (ALOX5AP) ariadne homolog (ARI) 1 AJOO9771 -- ariadne-2 (D. melanogaster) 1 AFO99149 - : : : : homolog (all-trans retinoic acid inducible RING finger) (ARI2) ARP1 (actin-related protein 1 X822O6 1, yeast) homolog A (centractin alpha) (ACTR1A) ARP2 (actin-related protein 9 AFOO6082 2, yeast) homolog (ACTR2) ARP2/3 protein compex S AFOO6085 Tactivated, W subunit 34 (ARC34) Arp2/3 protein compex 6 AFOO6084 monocyte subunit p41 (ARC41) stimulated Arp2/3 protein compex 1 AFOO6084 subunit p41 (ARC41)) (low match) Arp2/3 protein complex 20 AFO17807 subunit p16 (ARC16) Arp2/3 protein complex 2 AFOO6087 subunit p20 (ARC20) Arp2/3 protein complex 3 AFOO6086 W subunit p21(ARC21) ARP3 (actin-related protein 11 AFOO6083 W 3, yeast) homolog (ACTR3) arrestin, beta 2 (ARRB2) 1 AF106941 B, T, W arSA (bacterial) arsenite 1 AFO47469 B, T : : transporter, ATP-binding, homolog 1 (ASNA1) aryl hydrocarbon receptor 2 AFO44288 B nuclear translocator-like (ARNTL) aryl hydrocarbon receptor U31913 -- interacting protein (AIP) arylsulfatase A (ARSA) X521.51 Tactivated asialoglycoprotein receptor M11025 : 2 (ASGR2) asparaginyl-tRNA 3 D84273 synthetase (NARS) aspartyl-tRNA synthetase JOSO32 B (DARS) ataxiatelangiectasia U82828 B, T mutated (includes complementation groups A, C and D) (ATM) ataxin-2-like protein A2LP AFO34373 B, T (A2LG) activated ATF6 AFOOS887 ATP binding cassette U88667 transporter (ABCR) (non exact 80%) ATP synthase (F1-ATPase) XS9066 alpha subunit, mitochondrial US 2010/01 24746 A1 May 20, 2010 12

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu ATP synthase beta subunit 1 M19482 gene ATP synthase, H+ 1 X60221 ------transporting, mitochondrial FO complex, subunit b, isoform 1 (ATP5F1) ATP synthase, H+ 1 X69907 Tactivated ------transporting, mitochondrial FO complex, Subunit c (subunit 9), isoform 1 (ATP5G1) ATP synthase, H+ 3 D14710 transporting, mitochondrial F1 complex, alpha Subunit, isoform 1, cardiac muscle (ATP5A1) ATP synthase, H+ 1 D14710 transporting, mitochondrial F1 complex, alpha Subunit, isoform 1, cardiac muscle (ATP5A1) (low match) ATP synthase, H+ 2 M27132 ansporting, mitochondrial complex, beta olypeptide (ATP5B) ATP synthase, H+ 1 D16563 W ------ansporting, mitochondrial complex, gamma olypeptide 1 (ATP5C1) ATP synthase, H+ 1 AFO92124 ------transporting, mitochondrial F1 FO, subunit g (ATP5JG) ATP/GTP-binding protein 2 UT 3524 ------(HEAB) ATPase, Ca++ S Z69881 -- transporting, ubiquitous (ATP2A3) ATPase, H+ transporting, 2 D89052 ------ysosomal (vacuolar proton pump) 21 kD (ATP6F) ATPase, H+ transporting, 1 X76228 ------ysosomal (vacuolar proton pump) 31 kD (ATP6E) ATPase, H+ transporting, S X691.51 ------ysosomal (vacuolar proton pump) 42 kD; Vacuolar proton-ATPase, subunit C; V-ATPase, subunit C (ATP6D) ATPase, H+ transporting, 3 LO9235 -- -- ysosomal (vacuolar proton pump), alpha polypeptide, 70 kD, isoform 1 (ATP6A1) ATPase, H+ transporting, 6 X62949 ------ysosomal (vacuolar proton pump), beta polypeptide, 56/58 kD, isoform 2 (ATP6B2) ATPase, H+ transporting, 2 AFO38954 ------high in testis ysosomal (vacuolar proton pump), member J (ATP6J) ATPase, H+ transporting, 1 D16469 ------ysosomal (vacuolar proton pump), subunit 1 (ATP6S1) ATP-binding cassette 50 1 AFO273O2 ------(TNF-alpha stimulated) (ABC50) US 2010/01 24746 A1 May 20, 2010 13

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. O Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu ATP-binding cassette AFO47690 protein M-ABC1 (mitochondrial) ATP-dependent RNA AJO10840 T lymphoma -- -- helicase autoantigen (Hs.75528) LOS425 Tactivated -- autoantigen (Hs.75528) LOS425 (non-exact 84%) autoantigen (Hs.75682) U17474 -- autoantigen La SS-B Z35127 axin (AXIN1) AFOO9674 -- axonemal dynein heavy AJOOOS22 chain (DNAH17) BAI1-associated protein 3 ABO17111 (BAIAP3) (non-exact 54%) basement membrane AFO44896 induced gene (ICB1) basic leucine Zipper U79751 nuclear factor 1 (JEM-1) (BLZF1) basic transcription factor 3 ------(BTF3) basigin (BSG) L10240 -- -- BC-2 AFO42384 ------B-cell CLL/lymphoma 6 UOO115 -- -- (Zinc finger protein 51) (BCL6) B-cell translocation gene 1, X61123 -- anti-proliferative (BTG) BCL2 adenovirus E1B U15173 -- -- 9 kD-interacting protein 2 (BNIP2) BCL2 adenovirus E1B AFO67396 ------9 kD-interacting protein 3 ike (BNIP3L) beclin 1 (coiled-coil, ------myosin-like BCL2 interacting protein) (BECN1) beta-1,2-N- U15128 acetylglucosaminyltransferase I (MGAT2) beta-2-microglobulin (B2M) 63 S82297 ------hig h in invasive prostate tumor beta-hexosaminidase alpha M16411 chain (HEXA) beta-tubulin VOO599 ------hig h in many libraries beta-tubulin (non-exact, AFO70S61 76%) beta-tubulin, pseudogene JOO315 BING4 Z971.84 biotinidase (BTD) (non-eact 62%) biotinidase (BTD) (non UO3274 exact 70%) biotinidase (BTD) (non UO3274 exact, 56%) BIOTINIDASE P43251 PRECURSOR biphenyl hydrolase-like X81372 -- -- ( serine hydrolase) (BPHL) bone marrow stromal cell D21878 -- antigen 1 (BST1) box-dependent myc AFO43900 interacting protein isoform BIN1-10 (BIN1) US 2010/01 24746 A1 May 20, 2010 14

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu box-dependent myc 1 AFO43900 interacting protein isoform BIN1-10 (BIN1) (non-exact, 64%) brain myO47 protein 1 AFO636OS T -- branched chain keto acid 3 Z14093 T : : -- dehydrogenase E1, alpha polypeptide (maple syrup urine disease) (BCKDHA) BRCA1 associated protein 1 D87462 -- -- (ubiquitin carboxy erminal hydrolase) (BAP1) BRCA1, Rho7 and vatl 1 L78833 genes, and ipf35 breakpoint cluster region 2 AFO44773 protein, uterine leiomyoma, ; barrier to autointegration actor (BCRP1) breakpoint cluster region 2 AFO44774 -- -- protein, uterine leiomyoma, 2 (BCRP2) breast cancer anti-estrogen 1 U92715 resistance 3 (BCAR3) (non-exact 73%) bromodomain-containing 2 M91585 protein, 140 kD (peregrin) (BR140) Bruton's 1 U13424 agammaglobulinemia tyrosine kinase (Btk) Bruton's tyrosine kinase 1 UT8027 (BTK) Bruton's tyrosine kinase 1 UT8027 (BTK), alpha-D- galactosidase A (GLA), L44-like ribosomal protein (L44L) and FTP3 (FTP3) BS4 1 AF108083 BTG2 (BTG2) 6 YO9943 ------BTK region clone flip 1 UT8027 ------BTK region clone flip-3 1 UO1923 -- BUB3 (budding uninhibited 4 AFOS3304 ------by benzimidazoles 3, yeast) homolog (BUB3) butyrate response factor 1 X79067 ------(EGF-response factor 1) (BRF1) butyrophilin (BTF1) U90543 -- butyrophilin like receptor ABO2O625.1 CAG repeat containing U80744 (CTG4A) CAGH32 U80743 -- calcium channel, voltage M83566 dependent, L type, alpha 1D subunit (CACNA1D) (low match) calcium calmodulin AFO6976S -- -- dependent protein kinase (CaM kinase) II gamma (CAMK2G) calcium calmodulin AF101.264 B -- dependent protein kinase kinase (KIAAO787) calmodulin (=M19311) D45887 calmodulin 1 M27319 B -- -- (phosphorylase kinase, delta) (CALM1) calnexin (CANX) M948.59 T -- -- US 2010/01 24746 A1 May 20, 2010 15

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu calpain, large polypeptide S XO4366 ------L1 (CAPN1) calpain, large polypeptide S M23254 L2 (CANP2) calpain, Small polypeptide 1 XO4106 -- (CAPN4) calpastatin (CAST) 3 D16217 -- Calponin 2 2 D83735 -- calponin 2 (CNN2) 1 D83735 B, T : -- calponin 2 (CNN2) (low 1 D83735 score) calumenin (CALU) 3 AFO137.59 -- cAMP response element 4 LOS912 binding protein CRE-Bpa (H. GS165L15.1) cAMP-dependent protein 1 M90360 kinase type II (Ht:31) canicular multispecific 1 AFOO967O -- -- organic anion transporter (CMOAT2) capping protein (actin 6 US 6637 B, T filament) muscle Z-line, alpha 1 (CAPZA1) capping protein (actin 2 UO3269 filament) muscle Z-line, alpha 2 (CAPZA2) capping protein (actin 1 UO3271 -- filament) muscle Z-line, beta (CAPZB) capping protein (actin 8 M94345 -- filament), gelsolin-like (CAPG) carbamoyl-phosphate 1 D78586 -- synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD) carbonic anhydrase V. 1 L19297 -- mitochondrial (CA5) carboxypeptidase D (CPD) 3 U6SO90 carnitine acylcarnitine 1 Y103.19 -- translocase (CACT) Cas-Br-M (murine) 2 X571 10 -- ecotropic retroviral transforming sequence (cbl) casein kinase 1, alpha 1 1 L37042 -- (CSNK1A1) casein kinase 2, alpha 1 2 M55265 -- polypeptide (CSNK2A1) casein kinase I gamma 3L 1 AFO49090.1 (CSNK1G3L) casein kinase II alpha 1 X69951 subunit (=S72393) CASP8 and FADD-like 4 AFO15450 -- -- apoptosis regulator (CFLAR) caspase 1, apoptosis 7 U13697 -- -- related cysteine protease (interleukin 1, beta, convertase) (CASP1) caspase 10, apoptosis 1 U60519 B, T -- related cysteine proteas activated, T (CASP10) lymphoma caspase 3, apoptosis 3 U13737 B, T -- -- related cysteine protease (CASP3) US 2010/01 24746 A1 May 20, 2010 16

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu caspase 4, apoptosis 6 ------related cysteine protease (CASP4) caspase 5, apoptosis related cysteine protease (CASP5) caspase 8, apoptosis X981.73 -- related cysteine protease (CASP8) caspase 9, apoptosis US6390 -- -- related cysteine protease (CASP9) catalase (CAT) -- catechol-O- : : -- methyltransferase (COMT) catenin (cadherin D14705 associated protein), alpha 1 (102 kD) (CTNNA1) cathelicidin antimicrobial X89658 peptide (CAMP) cathepsin B (CTSB) L1651O -- cathepsin C (CTSC) U794.15 -- : cathepsin D (lysosomal M11233 : -- aspartyl protease) (CTSD) cathepsin E (CTSE) JOSO36 -- cathepsin G (CTSG) M16117 T, W cathepsin S (CTSS) M86553 B, Monocyte -- stimulated, T lymphoma cathepsin W (lymphopain) AFO13611 (CTSW) CBF1 interacting AFO98.297 corepressor CIR (=U03644 recepin) CCAAT enhancer binding X87248 -- protein (C/EBP), alpha (CEBPA) CCAAT enhancer binding S631.68 -- protein (C/EBP), delta (CEBPB) CCAAT-box-binding M371.97 T lymphoma -- -- transcription factor (CBF2) CCR5 receptor (CCR5) AFO11504 (non-exact?) CD14 antigen (CD14) M865.11 -- -- CD18 (=M95293) X64071 CD1C antigen, c s M28827 polypeptide (CD1C) CD2 antigen (cytoplasmic AF104222 tail)-binding protein 2 (CD2BP2) CD2 antigen (p50), sheep M14362 -- -- red blood cell receptor (CD2) D2 cytoplasmic tail AFO386O2 -- inding protein 1 (CD2BP1) D20 antigen (CD20) X12S30 D20 receptor (S7) XO72O3 D22 antigen (CD22) U62631 B D24 signal transducer MS8664 D33 antigen (gp67) M231.97 -- (CD33) CD33 antigen-like 2: OB U71383 binding protein-2 (CD33L2) (non-exact, 68%) CD33L2 (61% aa) D86359 US 2010/01 24746 A1 May 20, 2010 17

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

D36 antigen (collagen 7 M98398 T lymphoma ------pe I receptor, rombospondin receptor) D36) D37 antigen (CD37) 5 X14046 -- D38 at 1 D84277 D39 antigen (CD39) 1 U87967 B -- D3D antigen, delta 1 XO3934 polypeptide (TIT3 complex) (CD3D) CD3E antigen, epsilon 1 XO3884 -- polypeptide (TIT3 complex) (CD3E) CD3G antigen, gamma 2 XO6026 W polypeptide (TIT3 complex) (CD3G) CD37, antigen, Zeta 2 JO4132 -- polypeptide (TIT3 complex) (CD37) CD3-zeta (clone pBS NK1) 1 X55510 CD4 (low match) 1 S68043 CD4 antigen (p55) (CD4) 4 M12807 CD44 antigen (homing 6 XS6794 W : function and Indian blood group system (CD44) D48 antigen (B-cell 3 XO 6341 -- embrane protein) (CD48) D53 antigen (CD53) 10 L11670 -- D53 antigen (CD53) (low 1 M60871 atch) D63 antigen (melanoma 1 3 M59907 antigen) (CD63) CD68 antigen (CD68) 2 S57235 -- CD74 antigen (invariant 72 KO1144 -- : : : high in many libraries polypeptide of major histocompatibility complex, class II antigen-associated) (CD74) CD79A antigen 2 M80462 (immunoglobulin associated alpha) (CD79A) CD79B antigen 2 M89957 -- (immunoglobulin associated beta) (CD79B) CD8 antigen, alpha 2 M27161 -- polypeptide (p32) (CD8A) CD8 antigen, beta X13445 W polypeptide 1 (p37) (CD8B1) CD81 antigen (target of M3368O antiproliferative antibody 1 (CD81) CD83 antigen (activated B Q01151 B lymphocytes, mmunoglobulin Superfamily) (CD83) CD84 antigen (leukocyte U82988 antigen) (CD84) CD86 antigen L25259 CD9 antigen (p24) (CD9) 2 M38690 CD97 antigen (CD97) 12 X847OO -- CD97 antigen (CD97) P48960 (noin-exact 59%) CD97 antigen (CD97) (non X946.30 -- exact 62%) CDC23 (cell division cycle AFO53977 23, yeast, homolog) (CDC23) US 2010/01 24746 A1 May 20, 2010 18

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

CDC37 homolog 1 U63131 B -- -- Cdc42 effector protein 3 2 AF104857 B : : -- (CEP3) CDC-like kinase (CLK) 1 L292.19 -- CDC-like kinase 2 (CLK2) 1 AFO23268 B CDW52 antigen 13 X151.83 Tactivated -- (CAMPATH-1 antigen) (CDW52) cell cycle progression 1 AFO11794 restoration 8 protein(CPR8) cell division cycle 10 4 S72008 -- -- (homologous to CDC10 of S. cerevisiae) (CDC10) cell division cycle 20, 1 UOS340 -- S. cerevisiae homolog (CDC20) cell division cycle 25B 6 Z68092 -- -- (CDC25B) cell division cycle 2-like 1 1 AFO67514 (PITSLRE proteins) (CDC2L1) (non-exact 42%) cell division cycle 42 (GTP 5 M35543 -- -- binding protein, 25 kD) (CDC42) cell division protein (non 1 AFO 6301S exact 68%) CELL-CYCLE NUCLEAR 1 Q13033 AUTOANTIGENSG2NA (S/G2 NUCLEAR ANTIGEN) centromere protein B 1 X55039 -- (80 kD) (CENPB) cep250 centrosome 3 AFO226SS B -- associated protein ceroid-lipofuscinosis, 7 AFO17456 - -- -- high in bone neuronal 2, late infantile (Jansky-Bielschowsky disease) (CLN2) c-fgr (=M63877 6 XS22O6 nonreceptor protein tyrosine kinase (fgr)) CGI-19 protein 3 AF132953.1 chaperonin containing 1 X748O1 TCP1, subunit 3 (gamma) (CCT3) chaperonin containing 1 AFO26291 -- TCP1, subunit 4 (delta) (CCT4) chaperonin containing 4 L277O6 B TCP1, subunit 6A (Zeta 1) (CCT6A) chaperonin containing 4 AFO26292 B TCP1, subunit 7 (eta) (CCT7) Chediak-Higashi syndrome 1 U67615 B, T -- 1 (CHS1) lymphoma Chediak-Higashi syndrome 1 U67615 1 (CHS1) (low score) chemokine (C-C motif) 4 UO390S receptor 2 (CCR2) chemokine (C-C motif) 1 X85740 receptor 4 (CCR4) (low match) (may contain repeat) chemokine (C-C motif) 6 L31581 receptor 7 (CCR7) chemokine (C-X3–C) 5 U2O350 receptor 1 (CX3CR1) US 2010/01 24746 A1 May 20, 2010 19

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI H K Li Lu chemokine (C X-C motif), S M99293 ------receptor 4 (fusin) (CXCR4) hitinase 3-like 1 (cartilage 2 M80927 -- ycoprotein-39) (CHI3L1) hitinase 3-like 2 (CHI3L2) 2 U498.35 hloride channel 1, 1 G1828O keletal muscle (CLCN1) hloride channel 6 1 D28475 CLCN6) hloride intracellular 1 U932OS -- hannel 1 (CLIC1) hondroitin sulfate 5 X15998 roteoglycan 2 (versican) CSPG2) hondroitin sulfate 2 JO2814 roteoglycan core protein chromatin assembly factor 1 Q09028 1 p48 subunit (CAF-1 P4.8 Subunit) (retinoblastoma binding protein p48) (retinoblastoma-binding protein 4) (MSI1 protein homolog) chromodomain helicase 2 AFOO6513 DNA binding protein 1 (CHD1) chromodomain helicase 1 AFOS4177 DNA binding protein 1-like (CHD1L) chromodomain helicase 1 AFOO6514 B DNA binding protein 2 (CHD2) chromodomain helicase 1 AFOO6515 DNA binding protein 3 (CHD3) chromodomain helicase S X86691 -- DNA binding protein 4 (CHD4) chromosome 1 open AFOS4176 reading frame 7 (C1ORF7) chromosome 1 specific ABOO7962 transcript KIAAO493 chromosome 17 open AJOO8112 T reading frame 1B (C17ORF1B) chromosome 4 open AFOO6621 reading frame 1 (C4ORF1) chromosome condensation 2 AFO6O2.19 1-like (CHC1L) chromosome X open Y15164. B reading frame 5 (CXORF5) chromosome-associated 2 AFO92S64 B polypep i e C (CAP-C) ciga-2 AFO26944 cig5 3 AFO26941 citrate synthase (CS) 2 AFO47042 B class I maior 2 U31372 histocompatibility antigen (HLA-Cw3) class I maior 1 U31372 histocompatibility antigen (HLA-Cw3) (low match) clathrin assembly protein 3 U45976 B ymphoid myeloid leukemia (CALM) clathrin heavy chain 1 X55878 clathrin, heavy polypeptide 1 D21260 ike 2 (CLTCL2) US 2010/01 24746 A1 May 20, 2010 20

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu clathrin, light polypeptide 1 (Lca) (CLTA) (low match) clathrin 3 D63475 ------associated assembly adaptor protein, medium 1 (CLAPM1) cleavage stimulation factor, M85085 3' pre-RNA, subunit 2 64 kD (CSTF2) (non-exact 82%) cleavage stimulation factor, U15782 -- 3' pre-RNA, subunit 3, 77 kD (CSTF3) ck3 L2922O clone 23815 (Hs.82845) U90916 -- clone 24592 mRNA D88378 -- -- Sequence Cld/MBL/SPA receptor U94333 C1qR(p) () clusterin (complement lysis M64722 ------inhibitor, SP-40.40, Sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J) (CLU) CMP-sialic acid transporter D87969 B (CMPST) CMRF35 X66171 c-myc Oncogene containing XS4629 coxIII coagulation factor II M62424 -- (thrombin) receptor (F2R) coagulation factor V M1433S -- -- (proaccelerin, labile factor) (F5) coagulation factor XIIIa M21998 Subunit coagulation factor XIII, A1 -- -- polypeptide (F13A1) coated vesicle membrane X92098 ------protein (RNP24) coatomer protein complex, -- subunit alpha (COPA) Cofilin 1 (non-muscle) X954.04 ------high in fetal brain (CFL1) cold inducible RNA-binding D78134 -- protein (CIRBP) cold shock domain protein X953.25 A (CSDA) collagen, type IX, alpha 2 AFO194O6 B (COL9A2) colony stimulating factor 1 XO3663 -- -- receptor, formerly McDonough feline sarcoma viral (v-films) oncogene homolog (CSF1R) colony stimulating factor 2 MS9941 receptor, beta, low-affinity (granulocyte-macrophage) (CSF2RB) colony stimulating factor 2 MS9941 receptor, beta, low-affinity (granulocyte-macrophage) (CSF2RB) (low match) colony stimulating factor 3 16 receptor (granulocyte) (CSF3R) US 2010/01 24746 A1 May 20, 2010 21

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu complement component 5 1 M62SOS receptor 1 (C5a ligand) (C5R1) conserved gene amplified 2 AFOOO152 -- in osteosarcoma (OS4) COP9 (constitutive 2 AFO31647 photomorphogenic, Arabidopsis, homolog) subunit 3 (COPS3) COP9 homolog (HCOP9) 2 -- -- COPII protein, homolog of 4 : : S. cerevisiae SEC23p (SEC23A) copine I (CPNE1) U83246 -- copine I (CPNE1) (low U83246 score) coproporphyrinogen D16611 -- oxidase (coproporphyria, harderoporphyria) (CPO) core-binding factor, beta subunit (CBFB) coronin X89109 T, W -- coronin (low match) U34690 coronin (non-exact, 71%) X89109 cot (cancer Osaka thyroid) D14497 -- oncogene (COT) cryptochrome 1 D84657 (photolyase-like) (CRY1) CTD (carboxy-terminal AFO81287 -- domain, RNA polymerase II, polypeptide A) phosphatase, Subunit 1 (CTDP1) C-terminal binding protein U37408 -- 1 (CTBP1) C-terminal binding protein -- 2 (CTBP2) CUG triplet repeat, RNA U63289 -- binding protein 1 (CUGBP1) cullin 1 (CUL1) US 8087 -- cullin 3 (CUL3) -- cut (Drosophila)-like 1 (CCAAT displacement protein) (CUTL1) cyclin D2 (CCND2) D13639 -- cyclin D3 (CCND3) M92287 B, T -- lymphoma cyclin G1 (CNNG1) D78341 B cyclin I DSO310 B -- cyclin T2 (CNNT2) AFO48732 B, T B lymphoma cyclin-dependent kinase 2 (CDK2) cyclin-dependent kinase S76986 inhibitor (p27Kip1) cyclin-dependent kinase 2 S67388 -- -- inhibitor 1A (p21, Cip1) (CDKN1A) CYP2D7-CYP2D6 X90926 intergenic region (partial) cystatin B (stefin B) (CSTB) -- cysteine and glycine-rich L54.057 protein 3 (cardiac LIM protein) (CSRP3) cytidine deaminase (CDA) 2 L27943 -- US 2010/01 24746 A1 May 20, 2010 22

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu cytochrome b 1 AFO42SOO cytochrome b (CYTB) 1 AFO425.18 (isolate Aus5) cytochrome b(-245) beta 2 XO5895 chain N-terminal region (X- linked granulomatous disease gene) cytochrome b-245, beta XO4011 ------polypeptide (chronic granulomatous disease) (CYBB) cytochrome C POOOO1 cytochrome c oxidase U90915 T ------subunit IV (COX4) cytochrome c oxidase -- subunit Vb (COX5B) cytochrome c oxidase ABOO7618 ------subunit VII-related protein (COX7RP) cytokine Suppressive anti L35263 lymphocyte ------inflammatory drug binding protein 1 (p38 MAP kinase) (CSBP1) Cytoplasmic S69272 -- antiproteinase = 38 kda intracellular serine proteinase inhibitor cytotoxic granule S7.0114 associated RNA-binding protein p40-TIA-1 D123 (D123) D14878 ------D2-2 AFO 19226 D38 X748O2 damage-specific DNA AJOO295S ------binding protein 1 (127 kD) (DDB1) DCHT (low match) AFO1763S DEAD H (Asp-Glu-Ala U78524 ------Asp/His) box binding (DDXBP1) EAD/H (Asp-Glu-Ala U59321 T ------is) box polypeptide s (P72) i.AD/H (Asp-Glu-Ala ------is) box polypeptide 1 1) D.H. (Asp-Glu-Ala ABOO1636 is) box polypeptide DX15) D.H. (Asp-Glu-Ala ABO11149 ------is) box polypeptide 6) : (Asp-Glu-Ala U50553 ------p/His) box polypeptide 3 DX3) EAD/H (Asp-Glu-Ala 37 X15729 ------p/His) box polypeptide 5 RNA helicase, 68 kD) DX5) EAD/H (Asp-Glu-Ala AFO15812 p/His) box polypeptide 5 RNA helicase, 68 kD) DX5) (low match) EAD/H (Asp-Glu-Ala D17532 -- -- Asp/His) box polypeptide 6 (RNA helicase, 54 kD) (DDX6) US 2010/01 24746 A1 May 20, 2010 23

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

DEAD H (Asp-Glu-Ala 1 DSO487 ------Asp/His) box polypeptide 8 (RNA helicase, 54 kD) (DDX8) DEAD H (Asp-Glu-Ala 3 L13848 -- Asp/His) box polypeptide 9 (RNA helicase A, nuclear DNA helicase II: leukophysin) (DDX9) DEAD H (Asp-Glu-Ala 1 AFOOO985 -- Asp/His) box polypeptide, Y chromosome (DBY) Death associated protein 3 2 X83544 -- -- (DAP3) death effector domain 1 AFO83236 -- containing protein (DEDD) death-associated protein 6 2 AFO39136 -- (DAXX) dedicator of cyto-kinesis 2 4 D86964 -- (DOCK2) defender against cell death 1 D15057 -- 1 (DAD1) Defensin, alpha 1, myeloid 4 L12690 -- -- related sequence (DEFA1) DEK gene (D6S231E) 1 X64229 -- -- elta sleep inducing 4 ZSO781 -- peptide, immunoreactor (DSIPI) endritic cell protein 3 AFO 64603 -- (GA17) eoxycytidine kinase (DCK) 1 M60527 eoxyribonuclease II, 3 ABOO4574 lysosomal (DNASE2) DGS-I 2 L77566 iacylglycerol kinase 3 D16440 iacylglycerol kinase alpha 3 AFO64771 (DAGK1) (clone 24) iacylglycerol kinase alpha AFO64771 (DAGK1) (clone 24) (low match) iaphanous (Drosophila, AFOS1782 B, monocyte -- homolog) 1 (DIAPH1) stimulated iaphorase (NADH) YO95O1 ------cytochrome b-5 reductase) DIA1) ifferentiated Embryo ABOO4O66 -- Chondrocyte expressed gene 1 (DEC1) ifferentiated Embryo ABOO4O66 Chondrocyte expressed gene 1 (DEC1) (low match) ifferentiation antigen L2341S CD2O DiGeorge syndrome critical X84O76 region gene 2 (DGCR2) ihydrolipoamide 2 JO362O -- ehydrogenase (E3 component of pyruvate ehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid ehydrogenase complex) (DLD) ihydrolipoamide S 1 YOO978 -- acetyltransferase (E2 component of pyruvate ehydrogenase complex) (DLAT) US 2010/01 24746 A1 May 20, 2010 24

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu dihydropyrimidinase-like 2 1 D78O13 ------(DPYSL2) dinG gene 1 Y10571 diptheria toxin resistance 3 AFOS3003 B -- protein required for diphthamide biosynthesis (Saccharomyces)-like 2 (DPH2L2) disintegrin-protease (non 1 Y13323 exact 72%) DJ-1 protein 2 AFO21819 - -- -- Dmx-like 1 (DMXL1) 1 AJOOS821 -- -- DNA (cytosine-5-)- 3 X63692 Tactivated, -- methyltransferase 1 lymphoma (DNMT1) DNA fragmentation factor, 1 AFO 64019 40 kD, beta subunit (DFFB) DNA fragmentation factor, 2 U91985 T 45 kD, alpha subunit (DFFA) DNA mismatch repair 1 U17840 protein (hMLH1) DNA segment on 3 M64241 ------high in many libraries chromosome X (unique) 648 expressed sequence DNA segment, single copy 3 M73547 -- probe LNS-CAILNS-CAII (deleted in polyposis (DSS346) DNA-damage-inducible L24498 transcript 1 (DDIT1) (low match) DnaJ protein AJOO1309 DnaJ protein AJOO1309 ocking protein 2, 56 kD AFO34970 (DOK2) olichyl D89060 ------activated T cell iphosphooligosaccharide protein glycosyltransferase (DDOST) olichyl-phosphate D861.98 Tactivated -- mannosyltransferase polypeptide 1, catalytic subunit (DPM1) own-regulated by AJ2231.83 -- activation (immunoglobulin Superfamily) (DORA) own-regulated in P4O879 adenoma DRA (low match) D-type cyclin-interacting AFO82S69 B -- protein 1 (DIP1) ual specificity X68277 ------phosphatase 1 (DUSP1) ual specificity AFO23917 -- -- phosphatase 11 (RNA/RNP complex 1-interacting) (dusp11) ual specificity LOS147 -- phosphatase 3 (vaccinia virus phosphatase VH1 related) (DUSP3) ual specificity X939.20 ------phosphatase 6 (DUSP6) ynactin 1 (p150, Glued X988O1 (Drosophila) homolog) (DYTN1) US 2010/01 24746 A1 May 20, 2010 25

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

ynactin 1 (p150, Glued -- Drosophila) homolog) DYTN1) (low match) ynamin 2 (DNM2) L36983 ynamitin (dynactin USO733 complex 50 kD subunit) (DCTN-50) (non-exact 88%) ynein, axonemal, heavy X99947 polypeptide 17-like (non exact, 57% aa) ynein, cytoplasmic, light AFO35812 B intermediate polypeptide 2 ( DNCLI2) ynein, cytoplasmic, light AFO35812 intermediate polypeptide 2 DNCLI2) (non-exact, 69%) yskeratosis congenita 1, U591.51 B -- yskerin (DKC1) ystonia 1, torsion AFOOf871 -- autosomal dominant) DYT1) ystrobrevin, beta (DTNB) AFO22728 -- yStrophia myotonica L19267 -- containing WD repeat motif (DMWD) yStrophia myotonica LO883S -- protein kinase (DMPK) yStrophin (muscular X14298 yStrophy, Duchenne and Becker types) (DMD) (low match, 59% aa) E1B-55 kDa-associated -- protein E2F transcription factor 3 2 D385.50 -- -- (E2F3) E2F transcription factor 4, X86096 B -- p107p130-binding (E2F4) E2F transcription factor 5, 2 U15642 -- -- p130-binding (E2F5) E74-like factor 1 (ets M82882 B -- d O l 8.in transcription factor) (ELF1) ike factor 4 (ets 3 U32645 in transcription factor) (ELF4) E74-like factor 4 (ets 1 U32645 domain transcription factor) (ELF4) (non-exact, 71%) early development 4 U89278 -- -- regulator 2 (homolog of polyhomeotic 2) (EDR2) EBV induced G-protein 1 LO8.177 W coupled receptor (EBI2) ecotropic viral integration 3 M60830 -- site 2B (EVI2B) ectin, galactoside-binding, 1 JO4456 Soluble, 1 (galectin 1) (LGALS1) EGF-like-domain, multiple 1 ABO11541 4 (EGFL4) elF-2-associated pé7 3 U13261 B homolog elastin (Supravalvular aortic 1 M24782 stenosis, Williams-Beuren syndrome) (ELN) (low match) US 2010/01 24746 A1 May 20, 2010 26

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu elav-type RNA-binding 3 protein (ETR-3) electron-transfer 2 JO4058 flavoprotein, alpha polypeptide (glutaric aciduria II) (ETFA) ELK3, ETS-domain protein Z36715 -- -- (SRF accessory protein 2) (ELK3) elongation factor 1-beta L264.04 elongation factor Ts AF110399 (mitochondrial protein) elongation factor Tu X84694 nuclear encoded mitochondrial eMDC II protein AJ242O15.1 ems1 sequence (mammary M983.43 ------tumor and squamous cell carcinoma-associated (p80/85 Src substrate) (EMS1) endogenous retroviral Z70664 element HC2 endosulfine alpha (ENSA) X999.06 T endothelial differentiation, M31210 : ------sphingolipid G-protein coupled receptor, 1 (EDG1) endothelial differentiation, M31210 sphingolipid G-protein coupled receptor, 1 (EDG1) (low match 66%) endothelial monocyte U101.17 ------activating polypeptide (EMAPII) enolase 1, (alpha) (ENO1) M14328 -- enolase 2, (gamma, X51956 : neuronal) (ENO2) enolase-alpha D28437 enoyl Coenzyme A U16660 hydratase 1, peroxisomal (ECH1) enoyl Coenzyme A D13900 -- hydratase, short chain, 1, mitochondrial (ECHS1) ENOYL-COA HYDRATASE, MITOCHONDRIAL PRECURSOR (SHORT CHAIN ENOYL-COA HYDRATASE) (SCEH) (ENOYL-COA HYDRATASE 1) (low match, non-exact 56%) epidermal growth factor UO7707 -- -- receptor pathway Substrate 5 (EPS15) EPIDIDYMAL Q15668 SECRETORY PROTEIN E1 PRECURSOR (EPI-1) (HE1) (EPIDIDYMAL SECRETORY PROTEIN 4.6) (ESP14.6) epithelial membrane U87947 ------protein 3 (EMP3) Epoxide hydrolase 1, L29766 -- microsomal (xenobiotic) only (EPHX1) ERCC2 (=LA7234) XS2221 US 2010/01 24746 A1 May 20, 2010 27

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

ERF-2 3 UO78O2 ------high in gallbladder ERp28 protein 1 X94910 -- : ------erythrocyte membrane 2 M81 635 protein erythroleukemic cells K562 2 EST (Hs. 1895.09) 2 U24166 estrogen receptor-related L38487 protein (hERRal) ESTs, Highly similar to -- ADENYLOSUCCINATE SYNTHETASE ESTs, Moderately similar to U28811 ------cysteine-rich fibroblast growth factor receptor ET binding factor 1 (SBF1) U93181 -- -- ets domain protein ERF U15655 ------eukaryotic translation 326 XO3558 f -- -- ongation factor 1 alpha 1 E EF1A1) karyotic translation XO3558 ongation factor 1 alpha 1 EF1A1) (low match) karyotic translation XO3558 ongation factor 1 alpha 1 EF1A1) (low match) l karyotic translation S X60489 ------ongation factor 1 beta 2 E EF1B2) lE karyotic translation 1 Z21507 -- ongation factor 1 delta gll anine nucleotide xchange protein) (EEF1D) l karyotic translation 31 Z11S31 ongation factor 1 gamma E EF1G) l karyotic translation 2 XS1466 ongation factor 2 (EEF2) eukaryotic translation 1 JO2645 initiation factor 2, Subunit 1 (alpha, 35 kD) (EIF2S1) eukaryotic translation 1 M29536 initiation factor 2, Subunit 2 (beta, 38 kD) (EIF2S2) eukaryotic translation 3 L1916.1 initiation factor 2, subunit 3 (gamma, 52 kD) (EIF2S3) eukaryotic translation 2 U78311 initiation factor 3, Subuni 10 (theta, 150/170 kD) (EIF3S10) eukaryotic translation 3 U36764 -- high in white blood initiation factor 3, subunit 2 cells (beta, 36 kD) (EIF3S2) eukaryotic translation 6 US4559 -- high in spleen initiation factor 3, subunit 3 (gamma, 40 kD) (EIF3S3) eukaryotic translation ------initiation factor 3, Subunit 4 (delta, 44 kD) (EIF3S4) eukaryotic translation 4 U941.75 ------high in bladder initiation factor 3, subunit 6 (48 kD) (EIF3S6) eukaryotic translation 1 U62962 ------Highly represented initiation factor 3, subunit 6 (1.4833 pct) in library (EIF3S6) 36 human gall bladder US 2010/01 24746 A1 May 20, 2010 28

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu eukaryotic translation 3 U54558 ------initiation factor 3, subunit 7 (Zeta, 66/67 kD) (EIF3S7) eukaryotic translation S U46O2S ------high in testis initiation factor 3, subunit 8, 110 KD (EIF3S8) eukaryotic translation8 1 AFO12088 initiation factor 4 gamma, 1 (EIF4G) eukaryotic translation 1 AFO12088 initiation factor 4 gamma, 1 (EIF4G) (low match) eukaryotic translation 2 D12686 initiation factor 4 gamma, 1 (EIF4G1) eukaryotic translation 6 U73824 ------initiation factor 4 gamma, 2 (EIF4G2) eukaryotic translation 2 U76111 ------initiation factor 4 gamma, 2 (EIFG2) eukaryotic translation 29 D13748 initiation factor 4A, isoform 1 (EIF4A1) eukaryotic translation 11 D306SS ------initiation factor 4A, isoform 2 (EIF4A2) eukaryotic translation 18 X55733 -- -- initiation factor 4B (EIF4B) eukaryotic translation 1 PO6730 initiation factor 4E (EIF4E) Eukaryotic translation 3 L360S6 T, B -- initiation factor 4E binding protein 2 (EIF4EBP2) eukaryotic translation 2 Q15056 initiation factor 4H (EIF4H) eukaryotic translation 2 U494.36 ------initiation factor 5 (EIF5) eukaryotic translation 2 U901.76 -- -- termination factor 1 (ETF1) EV12 protein 1 MSS.266 Ewing sarcoma breakpoint 1 X66899 -- : -- region 1 (EWSR1) EWSFLI1 activated 2 AFO2O264 transcript 2 homolog (EAT 2) EWS-E1A-F chimeric 1 U3S622 protein excision repair cross 1 M286SO -- -- complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) (ERCC1) excision repair cross 1 X699.78 -- complementing rodent repair deficiency, complementation group 5 (xeroderma pigmentosum, complementation group G (Cockayne syndrome)) (ERCC5) exostoses (multiple)-like 3 1 AFOO1690 -- (EXTL3) F11 1 X77744 -- F1-ATPase beta subunit 2 XO3559 (F-1 beta) US 2010/01 24746 A1 May 20, 2010 29

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu Fanconianaemia group A 2 Z8309S Fanconi anemia, 1 X99226 ------complementation group A (FANCA) air upstream element 2 UOSO40 ------(FUSE) binding protein 1 (FUBP1) airnesyl diphosphate 1 JOS262 ------synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase) (FDPS) airnesyl-diphosphate 2 X69141 ------airnesyltransferase 1 (FDFT1) arnesyltransferase, CAAX 2 LOO63S -- -- box, beta (FNTB) Fasligand (gene and 1 AFO44583 promoter region) Fas-ligand associated 1 UTO667 actor 1 atty-acid-Coenzyme A 4 D10040 ------igase, long-chain 1 (FACL1) Fc fragment of IgA, 1 XS41SO receptor for (FCAR) Fc fragment of IgE, high 1 M331.9S ------affinity I, receptor for: gamma polypeptide (FCER1G) Fc fragment of IgE, low 2 XO4772 -- -- affinity II, receptor for (CD23A) (FCER2) Fc fragment of IgG, low 6 M31932 ------affinity IIa, receptor for (CD32) Fc fragment of IgG, low 1 X62572 ------affinity IIa, receptor for (CD32) (FCGR2A) Fc fragment of IgG, low 34 XO7934 ------affinity IIIa, receptor for (CD16) (FCGR3A) Fc fragment of IgG, 3 U12255 ------+ high in many libraries receptor, transporter, alpha (FCGRT) c-fgr 1 Z13983 Fc-gamma-receptorIIIB 2 M90746 (FCGR3B) eline sarcoma (Snyder- 3 XO6292 Theilen) viral (v- es). Fujinami avian sarcoma (PRCII) viral (v- ps) oncogene homolog(FES) c-fes?fps) emale sterile homeotic- 2 X96670 ------related gene 1 (mouse homolog) (FSRG1) erritin L-chain 9 YO9188 erritin, heavy polypeptide 1 4 M11146 ------(FTH1) ertilin alpha pseudogene 1 YO9232 etal Alzheimer antigen 2 UO5237 -- (FALZ) etal Ig heavy chain 1 M34024 variable region fibrillarin (FBL) 1 X56597 ------US 2010/01 24746 A1 May 20, 2010 30

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu fibrinogen-like protein 2 3 Z36531 (T49) fibroblast growth factor 1 M35718 -- receptor 2 (bacteria expressed kinase, keratinocyte growth factor receptor, craniofacial dysostosis 1, Crouzon syndrome) syndrome, Pfeiffer syndrome, ackson-Weiss) (FGFR2) icolin (collagen fibrinogen 19 D8392O domain-containing) 1 (FCN1) lamin A, alpha (actin 2 XS3416 sinding protein-280) ( FLNA) lamin B, beta (actin 1 AFO43O45 inding protein-278) FLNB) inkel-Biskis-Reilly murine 2 X65923 -- Highly represented in arcoma virus (FBR-MuSV) intraepithelial biquitously expressed (fox neoplasia and erived); ribosomal protein invasive prostate 30 (FAU) tumor K-506 binding protein 1 M8O199 ------K506-binding protein 1A 2 M34539 (12 kD) (FKBP1A) FK506-binding protein 1B 1 M92423 -- -- (12.6 kD) (FKBP1B) FK506-binding protein 5 4 U71321 ------BP5) htless I (Drosophila) 3 U80184 homolog (FLII) l less I (Drosophila) 1 U80184 O og (FLII) (low match) FLN29 (FLN29) 2 ABOOf447 -- -- lotillin 2 (FLOT2) S M60922 -- : ------olate receptor 2 (fetal) 1 AFOOO380 ------(FOLR2) orkhead (Drosophila) 1 AFO32886 ------homolog (rhabdomyosarcoma) like 1 (FKHRL1) Formyl peptide receptor 1 9 M60627 ------(FPR1) ormyl peptide receptor-like 1 M84562 Found only in (FPRL1) libraries from placenta ormyl peptide receptor-like 1 M84562 (FPRL1) (low score) ragile X mental retardation 1 L29074 ------(FMR1) ragile X mental 1 U251.65 ------retardation, autosomal homolog 1 (FXR1) Friend leukemia virus 3 M93255 -- integration 1 (FLI1) ructose-bisphosphatase 1 1 D26054 -- -- (FBP1) FSHD-associated repeat DNA, proximal region lucose-1-phosphate 1 AFO17445 -- -- guanylyltransferase (FPGT) ull length insert cDNA 1 AFO86122 clone ZA78A09 ull length insert cDNA 1 AFO75061 YPO7G10 US 2010/01 24746 A1 May 20, 2010 31

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu fumarate hydratase (FH) 1 ------FUS (low match) 1 FYN-binding protein (FYB 16 -- 120/130) (FYB) Galpha interacting protein X91809 (GAIP) (low score) G protein beta subunit-like D28398 protein 12.3 G protein-coupled receptor X81892 -- 64 (HE6) (non-exact 59%) G protein-coupled receptor L16862 -- -- kinase 6 (GPRK6) G1 to S phase transition 1 X17644 (GSPT1) GA-binding protein D13316 transcription factor, beta subunit 2 (47 kD) (GABPB2) galactose-1-phosphate M60091 uridylyltransferase (GALT) galactosidase, beta 1 M27508 -- -- (GLB1) galactosyltransferase M13701 (=X13223 N acetylglucosamide-(beta 1 4)-galactosyltransferase) galectin-9 isoform ABOO6782 ------gamma2-adaptin (G2AD) AFO 687O6 ------gamma-actin M371.30 gamma-aminobutyric acid AJO1218.7 -- -- (GABA) B receptor 1 (GABBR1) GATA-binding protein 2 M68891 -- -- (GATA2) GATA-binding protein 3 M69106 ------(GATA3) GCN5 (general control of D64007 ------amino-acid synthesis, yeast, homolog)-like 1 (GCNSL1) GDP dissociation inhibitor D45021 ------high in adult brain 1 (GDI1) GDP dissociation inhibitor Y13286 2 (GCI2) GDS-related protein U68142 ------(HKE1.5) gelsolin (amyloidosis, XO4412 Finnish type) (GSN) general transcription factor Y14946 , I (GTF2I) general transcription factor AFO38968 -- high in fetal brain , i, pseudogene 1 (GTF2IP1) general transcription factor X64037 ------F. polypeptide 1 (74 kD subunit) (GTF2F1) general transcription factor Z30093 H, polypeptide 3 (34 kD subunit) (GTF2H3) general transcription factor YO7595 -- -- H, polypeptide 4 (52 kD subunit) (GTF2H4) general transcription factor U14134 -- -- IA (GTF3A) general transcription factor UO2619 -- IC, polypeptide 1 (alpha subunit, 220 kD) (GTF3C1) US 2010/01 24746 A1 May 20, 2010 32

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI K Li Lu general transcription factor 3 D13636 ------IIIC, polypeptide 2 (beta subunit, 110 kD) (GTF3C2) germline immunoglobulin LO6612 heavy chain (IGHVG) germline immunoglobulin X92236 heavy chain, variabl region germline immunoglobulin X92343 heavy chain, variable region, (21-2) GLE1 (yeast homolog)-like, AFOS8922 RNA export mediator (GLE1L) glia maturation factor, beta ABOO1106 - (GMFB) glioma-associated oncogene homolog (Zinc finger protein) (GLI) glioma-associated XO7384 oncogene homolog (Zinc finger protein) (GLI) (low score) globin, alpha 2 WOOS16 glucocorticoid receptor M32284 (=M69104) glucocorticoid receptor 2 U80947 -- (GRL) glucos phosphate isomerase (CONTAINS LARGE REPEAT) glucosamine (N-acetyl)-6- Z12173 -- Sulfatase (Sanfilippo disease IIID) (GNS) glucosamine (N-acetyl)-6- Z12173 Sulfatase (Sanfilippo disease IIID) (GNS) (non e xact 56%) lucose transporter-like rotein-III (GLUT3) lucose transporter-like rotein-III (GLUT3) (low match) glucosidase, alpha; acid YOO839 -- (Pompe disease, glycogen storage disease type II) (GAA) glucosidase, beta; acid KO2920 -- (includes glucosylceramidase) (GBA) glutamate dehydrogenase 1 (GLUD1) glutamate-ammonia ligase 12 XS9834 -- (glutamine synthase) (GLUL) glutamate-ammonia ligase 1 YOO387 (glutamine synthase) (GLUL) (low score) glutamate-cysteine ligase 1 M90656 (gamma-glutamylcysteine synthetase), catalytic (72.8 kD) (GLCLC) utamine cyclotransferase X71125 utamine-fructose-6- M90516 hosphate transaminase 1 (GFPT1) 9. utaminyl-tRNA 1 X72396 synthetase US 2010/01 24746 A1 May 20, 2010 33

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu glutaminyl-tRNA 6 X76O13 ------synthetase (QARS) glutamyl-prolyl-tRNA 1 XS4326 synthetase (EPRS) glutathione peroxidase 1 2 M21304 -- (GPX1) glutathione peroxidase 4 X71973 -- (phospholipid hydroperoxidase) (GPX4) glutathione S-transferase pi (GSTP1) glutathione S-transferase Subunit 13 homolog yceraldehyde-3- 12 -- hosphate dehydrogenase GAPD) ycogenin (GYG) U31525 ------ycophorin C (Gerbich X12496 : ------ood group) (GYPC) ycoprotein M6B (GPM6B) U45955 -- ycyl-tRNA synthetase UO9587 : ------A.R S ) yoxalase I (lactoyl LO7837 ------glutathione lyase) (GLYI) golgi autoantigen, golgin US 1587 -- Subfamily a, 1 (GOLGA1) golgi autoantigen, golgin LO6147 Subfamily a, 2 (GOLGA2) (non-exact, 70%) golgi autoantigen, golgin U31906 Subfamily a, 4 (GOLGA4) golgi autoantigen, golgin X75304 ------Subfamily b, macrogolgin (with transmembrane signal), 1 (GOLGB1) gp25L2 protein X90872 grancalcin M81637 -- -- granulin (GRN) 1 X62320 -- : ------granulin (GRN) (low match) X62320 Granulysin (NKG5) M85276 -- -- granzyme A (granzyme 1, M18737 ------cytotoxic T-lymphocyte associated serine esterase 3) (GZMA) GRB2-related adaptor U52518 T only protein (GRAP) Grb2-related adaptor AFO90456 T -- protein 2 (GRAP2) GRO1 oncogene XS4489 -- -- (melanoma growth stimulating activity, alpha) (GRO1) growth arrest and DNA damage-inducible gene (GADD153) growth arrest-specific 7 ABOO7854 -- (GAS7) growth factor receptor X62852 B -- -- bound protein 2 (GRB2) GS1 (protein of unknown M86934 -- -- function) GS3955 D87119 ------GTP binding protein 1 U87964 -- -- (GTPBP1) GTP binding protein similar U87791 ------to S. cerevisiae HBS1 (HBS1) US 2010/01 24746 A1 May 20, 2010 34

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

GTPase activating protein 1 ABO11110 ------high fetal brain like (GAPL) GTP-binding protein (low 1 Z49068 match) GTP-binding protein G(K), 1 PO8754 alpha subunit (=G(I) ALPHA-3)(=GTP-binding regulatory protein Gi alpha 3 chain) Gu protein (GURDB) 2 U41387 -- guanine nucleotide binding 1 protein guanine nucleotide binding 4 JO3OO4 -- protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2) guanine nucleotide binding 7 M20597 -- protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3) guanine nucleotide binding 2 XO4409 B, T protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) guanine nucleotide binding 1 Z18859 protein (G protein), alpha transducing activity polypeptide 2 (GNAT2) guanine nucleotide binding 2 AFO17656 protein (G protein), beta 5 (GNB5) guanine nucleotide binding S M36430 -- protein (G protein), beta polypeptide 1 (GNB1) guanine nucleotide binding 2 AFO11496 protein (G protein), q polypeptide (GNAQ) guanine nucleotide binding 1 L2566S -- protein-like 1 (GNL1) guanine nucleotide 1 L13857 -- exchange factor guanine nucleotide 1 X15610 -- regulatory factor (LFP40) guanine nucleotide 1 U72206 -- regulatory factor (LFP40) GUANINE NUCLEOTIDE 1 P25388 BINDING PROTEINBETA SUBUNIT LIKE PROTEIN 12.3 (P205) (RECEPTOR OF ACTIVATED PROTEIN KINASEC 1) (RACK1) GUANINE 1 U10860 MONOPHOSPHATE SYNTHETASE (GMPS) guanosine monophosphate 1 M24470 reductase (GMPR) (non exact, 72%) guanosine-diphosphatase 1 AFO16032 like protein guanylate binding protein 2 M55542 1, interferon-inducible, 67 kD (GBP1) guanylate binding protein 6 MSSS43 -- 2, interferon-inducible (GBP2) H2A histone family, 1 Z83742 member C (H2AFC) US 2010/01 24746 A1 May 20, 2010 35

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br K Li Lu

H2A histone family, 2 AFO41483 ------member Y (H2AY) H2B histone family, 2 Z80783 ------high in adrenal gland member L. (H2BFL) tumor h2-calponin D86059 H-2K binding factor-2 LO8904 -- H3 histone family, member Z83735

H3 histone, family 3A M11353 -- -- high in ovary

3 histone, family 3B Z4.8950 -- -- high in endothelial cells U68494 -- -- at shock 27 kD pro ein 1 U12404 : : -- S PB1) eat shock 40 kD pro ein 1 D85429 ------high in testis HSPF1)S at shock 60 kD pro ein 1 M22.382 ------( C haperonin) (HSPD1 ) at shock 70 kD pro ein 1 MS9828 ------high in activated T SPA1A) cells heat shock 70 kD pro ein 5 13 X87949 -- (glucose-regulated protein, 78 kD) (HSPA5) heat shock 70 kD pro ein 6 X51757 -- heat shock 70 kD pro ein L151.89 -- -- 9B (mortalin-2) (HSPA9B) HEAT SHOCK COGNATE P11142 71 KD PROTEIN (8 SOCK factor bin ing AFO 687 S4 protein 1 (HSBP1) (8 SOC & protein 90 M27024 ------high in many libraries (8 SOC & protein, DNAJ D13388 : : -- -- ike 2 (HSJ2) Hect (homologous to le USOO78 -- E6-AP (UBE3A) carboxyl erminus) domain and RCC1 (CHC1)-like domain (RLD) 1 (HERC1) hect domain and RLD 2 ABOO2391 -- -- (HERC2) helicase-like protein (HLP) X98378 -- -- helix-loop-helix protein M65214 : HE47 (E2A) hematopoietic cell-specific X16663 -- -- Lyn Substrate (HCLS1) neme oxygenase XO6985 -- -- (decycling) 1 (HMOX1) HEMOGLOBINALPHA P19015 CHAIN hemoglobin beta (beta AF117710 globin) hemoglobin, alpha 1 30 WOO491 -- (HBA1) hemoglobin, alpha 1 WOO491 (HBA1) (low match) hemoglo bin, alpha 1 (low WOO493 match) hemoglobin, alpha 1 (non JOO153 exact, 76%) hemoglobin, alpha 1 (non WOO493 exact, 82%) hemoglobin, beta (HBB) 129 WOO497 ------high in many libraries hemoglobin, beta (HBB) WOO497 (low match) hemoglobin, beta (HBB) (low match) US 2010/01 24746 A1 May 20, 2010 36

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

hemokine (C X—C motif), 1 D10924 ------receptor 4 (fusin) (CXCR4) hemopoietic cell kinase S. M16591 -- (HCK) hepatitis C-associated 2 D28908 microtubular aggregate protein p44 hepatoma-derived growth 1 D16431 -- -- actor Hermansky-Pudlak 2 U65676 syndrome (HPS) HERV-E integrase (non 1 AFO26246 exact 76% aa) heterogeneous nuclear 2 S63912 -- protein similar to rathelix destabilizing protein (FBRNP) heterogeneous nuclear 4 M16342 ribonucleoprotein (C1/C2) (HNRPC) heterogeneous nuclear 1 M6SO28 ------ribonucleoprotein AB (HNRPAB) heterogeneous nuclear 2O X12671 ------High in alveolar ribonucleoprotein A1 rhabdomyosarcoma (HNRPA1) heterogeneous nuclear 3 M29064 ------High in activated T ribonucleoprotein A2B1 cell, fetal brain (HNRPA2B1) heterogeneous nuclear 2 D55673 ------ribonucleoprotein D (hnRNP D) heterogeneous nuclear S D89092 ------ribonucleoprotein D-like (HNRPDL) heterogeneous nuclear 1 L28O10 -- -- ribonucleoprotein (HNRPF heterogeneous nuclear 1 L28O10 ribonucleoprotein (HNRPF) (83%) heterogeneous nuclear 2 Z23064 -- ribonucleoprotein G (HNRPG) heterogeneous nuclear 3 P55795 ribonucleoprotein H (HNRPH) (FTP-3) heterogeneous nuclear 1 P31943 ribonucleoprotein H (HNRPH) (low match) heterogeneous nuclear 2 L22009 -- -- ribonucleoprotein H1 (H) (HNRPH1) heterogeneous nuclear 21 S74678 ------ribonucleoprotein K (HNRPK) heterogeneous nuclear 1 AFOOO364 -- -- ribonucleoprotein R (HNRPR) heterogeneous nuclear 3 X65488 ------ribonucleoprotein U (scaffold attachment factor A) (HNRPU) hexokinase 1 (HK1) 2 X66957 -- hexokinase 2 (HK2) 3 Z46376 -- : : -- : hexokinase 3 (HK3) 2 US 1333 hexosaminidase A (alpha 1 S62047 polypeptide) (HEXA US 2010/01 24746 A1 May 20, 2010 37

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu HGMPO7I gene for 2 U76377 olfactory receptor High density lipoprotein 2 M64098 ------binding protein (HDLBP) high-mobility group 5 X12597 ------(nonhistone chromosomal) protein 1 (HMG1) high-mobility group 1 D63874 (nonhistone chromosomal) protein 1 (HMG1) (non exact 60%) High-mobility group 2 M12623 ------(nonhistone chromosomal) protein 17 (HMG17) high-mobility group 2 M8366S ------(nonhistone chromosomal) protein 2 (HMG2) high-mobility group 2 L17131 ------(nonhistone chromosomal) protein isoforms I and Y high-risk humanpapilloma 1 AFO90990.1 viruses E6 oncoproteins argeted protein E6TP1 beta (=AB007900 KIAAO440) histidine ammonia-lyase 1 D16626 +, (HAL) only histidyl-tRNA synthetase 2 Z11S18 ------(HARS) histocompatibility antigen 1 U31372 (HLA-Cw3), class I histone deacetylase 1 4 USOO79 ------(HDAC) histone deacetylase 1 2 DSO4OS ------(HDAC1) histone deacetylase 5 (NY- AFO39691 -- -- CO-9) HK2 gene for hexokinase II Z46362 HL9 monocyte inhibitory 2 U91928 -- receptor precursor HLA class I heavy chain (HLA-Cw:1701) HLA class I locus Cheavy X58536 chain HLA class II SB 4-beta XO3O22 chain HLA class III region U8.9335 ------containing NOTCH4 gene HLA-A Z72423 HLA-A 2 AJOO6O20 HLA-A*74O2 AJ223060 HLA-A11 UO2934 HLA-B 2 X75953

HLA-B X78426 HLA-Bassociated Z37166 ------transcript-1 (D6S81E) HLA-Bassociated 2 M33509 ------transcript-2 (D6S51E) HLA-B81529 4 D445O1 HLA-BwT2 antigen 119 LO9736 ------+ high in many libraries HLA-C gene (HLA- 1 D83957 Cw:0701 allele)

HLA-Cw*1203 1 D64146 US 2010/01 24746 A1 May 20, 2010 38

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

HLA-DC classII 2 XOO370 histocompatibility antigens alpha-chain (=KO1160) HLA-DRalpha-chain M60333 ------high in spleen HLA-F (leukocyte antigen X17093 -- : : F) HMG box containing AFO 19214 protein 1 ABO17806.1 Hmob33 3 Y14155 HMT1 (hnRNP U802.13 ------methyltransferase, S. cerevisiae)- ike 1 (HRMT1L1) nRNP C1, C2 homeobox (=X58250 Mouse homeo box protein, put. transcription factor involved in embryogenesis and hematopoiesis) homeobox protein (HLX1) U14326 (=M60721) homeodomain-interacting AFOO4849 -- protein kinase 3 (HIPK3) homolog of Drosophila past AFOO1434 -- -- (PAST) homolog of yeast (S. cerevisiae) DSO916 -- -- ufd2 (UFD2) HPV 16 E1 protein binding U96131 -- -- protein HRIHFB2157 ABO15344 -- -- HRX-like protein YO8836 (=AFO10403 ALR) hsc70 gene for 71 kd heat YOO371 shock cognate protein HSPCO)12 AFO77036.1 HSPCO21 AFO772O7.1 HsPex13p U71374 htra,2-beta-2 U87836 -- -- U60644 hunc18b2 U63533 -- -- HUNKI Y12059 -- huntingtin-interacting AFO49528 brotein HYPAFBP11 (HYPA) hVps41p (HVPS41) U87309 hydroxyacyl-Coenzyme A UO4627 -- -- ehydrogenase 3-ketoacyl Coenzyme A hiolase enoyl-Coenzyme A hydratase (trifunctional protein), alpha Subunit (HADHA) hydroxyacyl-Coenzyme A D16481 -- -- dehydrogenase 3-ketoacyl Coenzyme A hiolase enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit (HADHB) hydroxysteroid (17-beta) U34879 -- ehydrogenase 1 ( HSD17B1) hypothetical protein hypothetical protein (ALOO8729) (d.J257A7.2) US 2010/01 24746 A1 May 20, 2010 39

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu hypothetical protein 1 U96629 (CIT987SK 2A8 1 chromosome 8) hypothetical protein (clone 1 AFOSSOO4 24640) hypothetical protein (clone 1 Z70222 CRFp507G2490). hypone ical protein 1 ALO22238 (d 1042K10.4) (non-exact 76%) hypothetical protein 2 ALO31432 (d.J465N24.1 similar to e dicted yeast and worm O teins) hypothetical protein 2 ALOO873O (d.J487J7.1.1) hypothetical protein 2 ALO23653 (d.J753P9.2) hypothetical protein ALOSO1311 (DKFZp586I111) hypothetical protein ALOO8729 (J257A7.2) hypothetical protein ABOO7900 (KIAAO440) (=AF026504 R. norvegicus SPA-1 like tein) hypothetical protein (L1H3' reg ion) hypothetical protein (S164) P49756 hypothetical protein (similar AF109907 O hrombospondin) (non exact 56%) hypothetical protein 3 hypothetical protein B U47926 (HSU47926) (non-exact, hypothetical protein from 3 USO532 -- BCRA2 region (CG005) hypoxia-inducible factor 1, 1 AFOSO115 alpha subunit (basic helix oop-helix transcription actor) (HIF1A) a-associated invarian 1 M13555 gamma-chain (clones ambda-y (1,2,3)) iduronate 2-sulfatase 2 MS8342 -- (Hunter syndrome) (IDS) g heavy chain V region L2O779 (=D11016) g heavy chain variable 2 M34024 region g heavy chain variable Z75378 region (VH4DJ) (clone T14.4) g heavy chain variable Z75392 region (VH4DJ) (clone

M12378 g Kappa S49007 G kappa light chain X63398 variable region A20 gkappa light chain, V- and D90158 -region (=X59315) glambda light chain Z85052 variable region (26 34ITIIIF120) US 2010/01 24746 A1 May 20, 2010 40

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu g mu-chain VDJ4-region 1 M16949 grearranged anti-myelin 1 M29469 kappa-chain (V-J4-region, hybridoma AE6-5) grearranged H-chain 2 M9792O mRNAV-region grearranged light-chain V M74O2O region (=D90158) GF-II mRNA-binding U971.88 ------protein 3 (KOC1) (non exact, 75%) gGFc-binding protein D84239 ------(FC(GAMMA)BP) gG heavy chain variable M83136 region (vH26) gM heavy chain (C mu, X14939 membrane exons) kB kinase-beta (IKK-beta) AFO29684 L-1 receptor type II U14177 L2-inducible T-cell kinase 2 S6S186 (ITK) immediate early protein M62831 ------(ETR101) immunogloblin light chain D870 18 (lambda) Immunoglobulin (CD79A) YO8915 B, T ------binding protein 1 (IGBP1) immunoglobulin C (mu) 2 X573.31 and C (delta) heavy chain (=KO2878) immunoglobulin G Fc Z46223 receptor IIIB immunoglobulingamma 3 3 Y14.737 -- -- + high in many libraries (Gm marker) (IGHG3) immunoglobulingamma Z66542 heavy chain variable region (=X61011) immunoglobulin heavy X62109 chain (VI-3B) immunoglobulin heavy X86356 chain J region immunoglobulin heavy 2 X86355 chain J region, B haplotype immunoglobulin heavy AFO62126 chain variable region (IGH) (clone 21u-48) immunoglobulin heavy AFO62212 chain variable region (IGH) (clone 23u-1) immunoglobulin heavy 2 M99641 hain variable region V1-18 mmunoglobulin heavy 2 M99672 hain variable region V3-43 GHV(a)) mmunoglobulin heavy 3 M99649 hain variable region V3-7 GHV(a)) :mmunoglobulin IgEI heavy 1 UO7986 hain Fc fragmen N t l O9. obulin kappa light 1 X58O81 l l Og obulin kappa light 1 X12686 hain V-segment A27 N l Og obulin light chain 1 D86990 l l Og obulin light chain 1 D86996 ow match) US 2010/01 24746 A1 May 20, 2010 41

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu immunoglobulin light chain 1 L29157 variable region (lambda IIIb Subgroup) from IgM rheumatoid factor immunoglobulin Mheavy 1 S50735 chain V region = anti-lipid A antibody immunoglobulin mu (IGHM) 9 X57086 ------immunoglobulin mu binding 1 L24544 T : -- protein 2 (IGHMBP2) immunoglobulin 1 Z33642 Superfamily, member 2 (IGSF2) Immunoglobulin VH mRNA 1 X61013 (487 bp) (=M99652 immunoglobulin heavy chain variable region V3-11 (IGHV(a)) imogen 38 (IMOGEN38) 1 Z68747 ------IMP (inosine 1 JO5272 ------monophosphate) dehydrogenase 1 (IMPDH1) IMP (inosine 2 L39210 ------monophosphate) dehydrogenase 2 (IMPDH2) inc finger protein 151 (pHZ 1 YO9723 ------67) (ZNF151) inc finger protein, C2H2, 1 AFO11573 -- rapidly turned over (ZNF20) inducible poly(A)-binding 1 U33818 ------protein (IPABP) inducible poly(A)-binding 1 U33818 protein (IPABP) (low match) inducible protein 2 L47738 ------(Hs.80313) inhibitor of DNA binding 2, 4 M97796 -- dominant negative helix loop-helix protein (ID2) inhibitor of kappa light 2 AFO44-195 polypeptide gene enhancer in B-cells, kinase complex associated protein (IKBKAP) inositol 1,3,4-trisphosphate US 1336 -- 5.6-kinase inositol 14.5 trisphosphate U238SO -- -- receptor type 1 (ITPR1) inositol 1,4,5-trisphosphate 2 X572O6 B -- -- 3-kinase B (ITPKB) inositol monophosphatase S3898O inositol polyphosphate-5- 2 U844OO ------phosphatase, 145 kD (INPP5D) Ins(1,3,4,5)P4-binding X89399 -- protein insulin-like growth factor 2 5 YOO285 ------receptor (IGF2R) integral membrane protein L38961 ------1 (ITM1) integral membrane protein AFO3895.3 T ------2C (ITM2C) integral membrane protein 3 U61734 -- Tmp21-I (p23) integrin beta 4 binding 2 AFO47433 protein (ITGB4BP) US 2010/01 24746 A1 May 20, 2010 42

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu integrin, alpha2b (platelet 3 M3448O -- glycoprotein IIb of IIb, IIIa complex, antigen CD41B) (ITGA2B) integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5) integrin, alpha L (antigen YOO796 CD11A (p180), lymphocyte function-associated antigen 1: alpha polypeptide) (ITGAL) integrin, alpha M M18044 (complement componentreceptor 3, alpha; also known as CD11b (p170), macrophage antigen alpha polypeptide) (ITGAM) integrin, alpha X (antigen M81695 CD11C (p150), alpha polypeptide) (ITGAX) integrin, beta 1 (fibronectin XO7979 receptor, beta polypeptide, antigen CD29 includes MDF2 MSK12) (ITGB1) integrin, beta 2 (antigen 32 M15395 CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit) (ITGB2) integrin, beta 7 (ITGB7) M68892 Integrin-linked kinase (ILK) U4O282 : -- -- intercellular adhesion JO3132 molecule 1 (CD54), human rhinovirus receptor (ICAM1) intercellular adhesion X15606 molecule 2 (ICAM2) intercellular adhesion X698.19 molecule 3 (ICAM3) intercellular adhesion L27670 molecule 4, Landsteiner Wiener blood group (ICAM4) Interferon consensus M91196 W, T sequence binding protein 1 lymphoma (ICSBP1) Interferon consensus M91196 sequence binding protein 1 (ICSBP1) (low match) interferon regulatory factor X15949 2 (IRF2) interferon regulatory L05072 factor1 (IRF1) interferon regulatory U51127 factors (IRF5) interferon, gamma M63838 inducible protein 16 (IFI16) interferon, gamma JO3909 inducible protein 30 (IFI30) INTERFERON-INDUCED P32455 GUANYLATE-BINDING PROTEIN 1 (GUANINE NUCLEOTIDE-BINDING PROTEIN 1) (non-exact 62%) US 2010/01 24746 A1 May 20, 2010 43

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu interferon-induced protein 3 X84958 ------17 (IFI17) interferon-induced protein S. M14660 54 (IFI54) interferon-inducible (1-8D) 5 X57351 T ------interferon-inducible (1-8U) 1 X57352 ------interferon-related 5 Y10313 ------developmental regulator 1 (IFRD1) interferon-stimulated 2 M87503 ------transcription factor 3, gamma (48 kD) (ISGF3G) interleukin 1 receptor, type 1 U64O94 -- II (IL1R2) Interleukin 10 receptor, 1 UO8988 Tactivated -- -- beta (I.10RB) interleukin 12 receptor, 2 UO31.87 -- only found in T cell beta 1 (IL12RB1) interleukin 13 receptor, 2 YO9328 ------alpha 1 (IL13RA1) interleukin 16 (lymphocyte 6 U82972 -- chemoattractant factor) (IL16) interleukin 18 receptor 1 1 U43672 (IL18R1) interleukin 2 receptor, beta 9 M26062 (IL2RB) interleukin 2 receptor, 6 D11086 ------gamma (severe combined immunodeficiency) (IL2RG) interleukin 4 receptor 3 X5242S ------(IL4R) interleukin 6 receptor S X12830 -- -- (IL6R) interleukin 6 signa 1 M57230 transducer (gp130, oncostatin M receptor) (IL6ST) interleukin 7 receptor 14 M29696 -- -- (IL7R) interleukin 7 receptor 1 AFO43123 (IL7R) (low match) interleukin 8 (IL8) 8 YOO787 ------High in activated T cells, bone and pancreatic islets interleukin 8 receptor alpha 11 L19591 (IL8RA) interleukin 8 receptor, beta 14 M94582 (IL8RB) interleukin enhancer 3 U10323 ------+ high in uterus binding factor 2, 45 kD (ILF2) interleukin enhancer 2 U10324 binding factor 3,90 kD (ILF3) interleukin-1 receptor- 2 L76.191 ------associated kinase 1 (IRAK1) interleukin-1 receptor- 1 US 2112 associated kinase 1 (low match) interleukin-10 receptor, 5 UOO672 ------alpha (IL10RA) interleukin-11 receptor, 7 Z38102 -- -- alpha (IL11RA) US 2010/01 24746 A1 May 20, 2010 44

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

INTERLEUKIN-14 1 PRECURSOR(IL-14) (HIGH MOLECULAR WEIGHT B-CELL GROWTH FACTOR) (HMW-BCGF) (non-exact 46%) intestinal carboxylesterase: U60553 -- liver carboxylesterase-2 (ICE) inversin protein (non-exact AFO84367 52%) IQ motif containing L33075 GTPase activating protein 1 (IQGAP1) IQ motif containing US 1903 -- GTPase activating protein 2 (IQGAP2) isocitrate dehydrogenase 1 AFO2OO38 ------(NADP+), soluble (IDH1) isocitrate dehydrogenase 2 X69433 ------(NADP+), mitochondrial (IDH2) isocitrate dehydrogenase 3 UO7681 (NAD+) alpha (IDH3A) isocitrate dehydrogenase 3 Z68907 -- -- (NAD+) gamma (IDH3G) isolate Aus3 cytochrome b A. FO42516 (CYTB) isolate TZCCR5-179 CCR5 A. FO11524 receptor (CCR5) isopentenyl-diphosphate X 7025 -- -- delta isomerase (IDI1) anus kinase 1 (a protein M 64174 -- -- tyrosine kinase) (JAK1) anus kinase 2 (a protein A. FOOS216 tyrosine kinase) (JAK2) k-recombination signal LO7876 binding protein (RBPJK) M1 protein A. OOS890 -- jumonji (mouse) homolog U57592 : -- (JMJ) jun D proto-oncogene XS1346 -- -- (JUND) jun dimerization protein A. F111167 only found in germ junction plakoglobin (JUP) M23410 ------kangai 1 (Suppression of U20770 ------tumorigenicity 6, prostate; CD82 antigen (R2 eukocyte antigen, antigen detected by monoclonal and antibody A4)) (KAI1) karyopherin (importin) beta L39793 ------(KPNB1) karyopherin (importin) beta U72395 -- -- 2 (KPNB2) karyopherin alpha 1 S75295 -- -- importin alpha 5) (KPNA1) karyopherin alpha 2 (RAG UO9559 cohort 1, importin alpha 1) (DPNA2) karyopherin alpha 3 D89618 -- importin alpha 4) (KPNA3) karyopherin alpha 4 M 17887 (KPNA4) Katanin (80 kDa) (KAT) 1 AFOS2432 -- KE03 protein AFO 64604 US 2010/01 24746 A1 May 20, 2010 45

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

Kelch-like ECH-associated DSO922 protein 1 (KIAA0132) (66% aa) Keratin 8 (KRT8) X74929 ------ketohexokinase X78678 ------(fructokinase) (KHK) KIAAO001 (KIAAO001) Q15391 (72% aa) KIAAO001 (KIAAO001) Q15391 (76% aa) KIAAO001 (KIAAO001) Q15391 (non-exact 72%) KIAAO002 (KIAAO002) 5 D13627 ------KIAAO005 (KIAAO005) 4 D13630 ------KIAAO010 (KIAAO010) D1363S -- -- KIAAO016 (KIAAO016) D13641 ------KIAAO017 (KIAAO017) 2 D876.86 KIAAO022 (KIAAO022) 2 D14664 ------KIAAO023 (KIAAO023) D14689 -- KIAAO024 (KIAAO024) D14694 ------KIAAO025 (KIAAO025) D1469S ------KIAAO026 (KIAAO026) 2 D14812 ------KIAAO027 D25217 -- KIAAO032 (KIAAO032) 2 D25215 ------KIAA0040 (KIAA0040) D25539 ------KIAAO050 (KIAAO050) 4 D26069 KIAAO053 (KIAAO053) 17 D29642 ------KIAAO057 (KIAAO057) D31762 ------+ high in fetal lung KIAAO058 (KIAAO058) 11 D31767 ------KIAAO063 (KIAAO063) 3 D31884 ------KIAAO064 (KIAAO064) D31764 ------KIAAO066 D31886 ------KIAAO068 D38549 ------KIAAO073 3 D38552 ------KIAAO081 2 D42O39 ------KIAAO084 2 D42O43 ------KIAAO085 26 U3O498 ------KIAAO088 3 D42O41 ------KIAAO090 2 D42044 ------KIAAO092 (KIAAO092) D42O54 ------KIAAO094 3 D42O84 -- -- KIAAO095 (KIAAO095) D42O8S KIAAO096 D436.36 ------KIAAO097 (KIAAO097) X92474 T ------KIAAO099 (KIAAO099) 3 D43951 ------KIAAO102 (KIAA0102) 2 D14658 ------KIAAO105 D14661 B ------KIAAO120 2 P378O2 KIAAO120 (non-exact, M831O6 65%) KIAAO121 (KIAAO121) DSO911 ------KIAAO123 D21064 ------KIAAO128 DSO918 ------KIAAO129 (KIAAO129) DSO919 ------KIAAO130 (KIAAO130) AFO55995 ------KIAAO136 2 DSO926 KIAAO137 (KIAAO137) ABOO4.885 ------KIAAO140 (KIAAO140) DSO930 ------KIAAO141 (KIAAO141) 3 DSO931 KIAAO144 (KIAA0144) 3 D63478 ------KIAAO144 (KIAA0144) (low D63478 match) KIAAO144 (non-exact 61%) Q14157 KIAAO144 (non-exact 65%) Q14157 KIAAO146 2 D63480 ------KIAAO148 (KIAAO148) D63482 -- -- KIAAO154 2 D63876 ------KIAAO156 D63879 ------

US 2010/01 24746 A1 May 20, 2010 47

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu AA0379 (non-exact, 1 A. BOO2377 65%) AA0380 (KIAA0380) 1 BOO2378 AA0380 (KIAA0380) 1 BOO2378 0% aa) AA0382 (KIAA0382) BOO238O AAO383 BOO2381 AA0386 (KIAA0386) BOO2384 AAO392 BOO2390 AA0397 (KIAA0397) BOO7857 AAO403 BOO7863 AAO404 BOO7864 AAO409 BOO7869 : AA0421 BOO7881 -- -- AA0424 (non-exact 82%) BOO7884 AA0428 (KIAA0428) 3829 AA0429 (KIAA0429) BOO7889 ------AA0430 (KIAA0430) BOO7890 only in ovary AA0432 (KIAA0432) 86753 -- AA0435 (KIAA0435) BOO7895 AA0438 (KIAA0438) BOO7898 ------AA0447 (KIAA0447) BOO7916 ------AAO449 BOO7918 -- AAO456 BOO7925 ------AA0458 (KIAA0458) BOO7927 AAO462 BOO7931 ------AAO465 BOO7934 ------AA0476 (KIAA0476) BOO7945 -- -- AAO489 BOO7958 AA0494 (KIAA0494) BOO7963 ------AAOS 15 BO O87 ------AAOS21 BO O93 -- AAOS25 BO 097 -- AAO530 BO O2 -- -- AAOS32 BO O4 ------AAO537 (KIAA0537) BO 09 AAOS40 BO 12 ------AAOS43 BO 15 ------AAOS44 BO 16 ------AAOS49 BO 21 ------AAOSS1 : BO 23 -- AAO554 BO 26 ------AAOS 61 BO 33 -- AAO562 (KIAAO562) BO 34 AAO563 (KIAAO563) BO 35 AAO569 (KIAAO569) BO 41 ------AA0571 (KIAA0571) BO 43 -- -- AAOS73 BO 45 -- -- AAOS76 BO 48 AAOS80 BO 52 AAOS84 BO 56 AAO592 BO 64 ------AAO596 BO 68 -- AA0598 (KIAAO598) BO 70 -- -- AAO608 BO 8O -- -- AA0614 BO 4514 ------AA0615 (KIAA0615) BO 4515 AAO621 BO 4521 -- -- AAO648 BO 4548 ------AAO652 (KIAA0652) BO 4.552 : ------AAO668 BO 4568 AAO669 BO 4569 AA0671 (KIAAO671) BO 4571 ------AAO675 (KIAAO675) BO 4575 -- -- AAO676 BO 4576 ------AAO677 (KIAAO677) BO 4577 ------AAO678 BO 4578 ------AAO679 BO 4579 ------US 2010/01 24746 A1 May 20, 2010 48

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu AA0680 (KIAAO680) BO1458O AAO692 BO14592 -- AAO697 BO14597 AAO699 BO14599 AAO700 BO14600 AAO737 (KIAA0737) FO14837 AA0748 (KIAA0748) BO18291 AA0763 (KIAAO763) BO18306 AA0769 (KIAAO769) BO18312 : : AAO782 BO1832S high in BPH stroma AAO796 BO18339 AAO798 (KIAAO798) BO18341 AAO823 BO2O630 AAO854 BO2O661 AAO856 BO2O663 : : AAO860 BO2O667 AAO862 FOS4828 AAO871 (non-exact 88%) BO2O678 AAO873 BO2O68O AAO892 B020699 AAO906 BO2O713 : AAO991 B023208.1 killer cell lectin-like 1276 receptor subfamily B, member 1 (KLRB1) killer cell lectin-like U96846 receptor subfamily C, member 4 (KLRC4) kinectin 1 (kinesin receptor) D13629 (KTN1) kinesin family member 5B X65873 (KIFSB) kinesin-like DNA binding ABO17430 protein Krueppel-related DNA M61869 binding protein (TF6) (low match) Kruppel related gene (clone pHKR1 RS) Kruppel-like Zinc finger US1869 protein Zf Kruppel-like Zinc finger U44975 protein Zf (non-exact 76%) kruppel-type Zinc finger ABO11414.1 protein, ZK1 Lapoferritin XO3742 actate dehydrogenase A XO2152 (LDHA) actate dehydrogenase A XO2152 (LDHA) (non-exact, 81%) actate dehydrogenase B X13794 high in fetal lung (LDHB) fibrablast actotransferrin (LTF) UO7643 high in bone marrow aminin binding protein (low D28372 score) aminin receptor 1 (67kD); X15005 high in many libraries Ribosomal protein SA (LAMR1) aminin receptor homolog S35960 {3' region} aminin, gamma 1 (formerly -- LAMB2) (LAMC1) atent transforming growth M34.057 actor beta binding protein (LTBP1) LAZ3, BCL6 Z79581 (=Z79582: D28522/4) US 2010/01 24746 A1 May 20, 2010 49

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

LDLC 2 Z34975 ------ecithin-cholesterol 1 M17959 acyltransferase (LCAT) (non-exact, 66%) ectin, galactoside-binding, 1 M87842 -- Soluble, 2 (galectin 2) (LGALS2) ectin, galactoside-binding, 1 L13210 -- -- Soluble, 3 binding protein (galectin 6 binding protein) (LGALS3BP) eucine rich repeat (in FLII) 5 AJ223075 ------interacting protein (LRRFIP1) eucocyte immunoglobulin 2 AFO72099 -- ike receptor-5 (LIR-5) eucocyte immunoglobulin 7 AFO25530 ike receptor-6a (LIR-6) eucocyte immunoglobulin 2 U82275 only found in CNS ike receptor-7 (LIR-7) eukemia virus receptor 1 1 L2O859 -- -- (GLVR1) eukocyte adhesion protein 1 M29484 p150.95 alpha subunit eukocyte antigen, HLA-A2 3 Y13267 eukocyte immunoglobulin 3 AFO2SS28 ike receptor (MIR-10) eukocyte tyrosine kinase 1 X6O702 -- found only in blood (LTK) eukocyte-associated Ig 3 AFO13249 -- ike receptor 1 (LLAR1) eukotriene A4 hydrolase 6 JO3459 ------(LTA4H) eupaxin (LDPL) 2 AFO62O75 -- -- igase I, DNA, ATP 1 M36067 -- dependent (LIG1) LIM and SH3 protein 1 2 X82456 ------(LASP1) LIM domain kinase 2 2 ACOO2O73 -- -- (LIMK2) ine-1 protein Line-1 repeat mRNA with 2 U93566 ------open reading frames Line-1 repeat with 2 open 1 M22332 ------high in gastric tumor reading frames LINE-1 REVERSE 1 PO8547 TRANSCRIPTASE HOMOLOG ipase A, lysosomal acid, 4 X76488 -- -- cholesterol esterase (Wolman disease) (LIPA) ipase, hormone-sensitive 1 L117O6 -- (LIPE) LMP7 1 L11045 Lon protease-like protein X74215 -- -- (LONP) ow density lipoprotein 2 AFOS8414 -- only in liver related protein 1 (alpha-2- macroglobulin receptor) (LRP1) ow density lipoprotein 1 M63959 -- related protein-associated protein 1 (alpha-2- macroglobulin receptor associated protein 1) (LRPAP1) US 2010/01 24746 A1 May 20, 2010 50

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu ow density lipoprotein 1 M63959 related protein-associated protein 1 (alpha-2- macroglobulin receptor associated protein 1) (LRPAP1) (non-exact, 75%) ow-affinity Fc-gamma LO8107 receptor IIA LPS-induced TNF-alpha AFO10312 actor (PIG7) Lst-1 UOO921 L-type amino acid AF104032 transporter subunit LAT1 ung resistance-related X79882 protein (LRP) Lymphocyte antigen 75 AFO11333 (LY75) ymphocyte antigen 9 (LY9) L42621 ymphocyte antigen HLA L42345 B*44O2 and HLA-B*S101 ymphocyte cytosolic 42 JO2923 protein 1 (L-plastin) (LCP1) ymphocyte cytosolic T protein 2 (SH2 domain lymphoma, containing leukocyte Tactivated protein of 76 kD) (LCP2) ymphocyte glycoprotein -- T1. Leu-1 ymphocyte-specific protein 16 M33552 -- (LSP1) ymphocyte-specific protein M36881 tyrosine kinase (LCK) ymphoid phosphatase AFOO1847 LyP1 ymphoid-restricted U10485 membrane protein (LRMP) ymphoid-specific SP100 homolog (LYSP100-A) ymphoma proprotein U33849 convertase (LPC) LYSOSOMAL P10619 PROTECTIVE PROTEIN PRECURSOR (CATHEPSINA) (CARBOXYPEPTIDASEC) ysosomal-associated JO4182 membrane protein 1 (LAMP1) Lysosomal-associated JO41.83 membrane protein 2 (LAMP2) ysozyme (renal 39 M19045 amyloidosis) (LYZ) ysyl-tRNA synthetase (KARS) Mphase phosphoprotein X98494 O (U3 Small nucleolar ribonucleoprotein) (MPP O) M1-type and M2-type XS6494 pyruvate kinase móA methyltransferase AFO 14837 (MTA70) mab-21 (C. elegans)-like 1 U38810 (MAB21L1) MacMarcks X70326 US 2010/01 24746 A1 May 20, 2010 51

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu macrophage-associated 1 Z22968 ------antigen (MM130) MADS box transcription 1 U4902O ------enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) (MEF2A) MADS box transcription 1 LO8895 enhancer factor 2, polypeptide C (myocyte enhancer factor 2C) (MEF2C) major cytoplasmic tRNA 1 X17516 Val(IAC) (=M33940) major histocompatibility 1 M95531 complex class I beta chain (HLA-B) major histocompatibility 41 Z93949 -- -- high in villous complex, class I, A (HLA-A) adenoma major histocompatibility 1 Z72422 complex, class I, A (HLA-A) major histocompatibility 82 M24097 -- complex, class I, C (HAL

compatibility 77 M20022 -- ass I, E. (HLA-E) compatibility 2 U15085 -- complex, class II, DM BETA (HLA-DMB) major histocompatibility 10 MS7466 -- complex, class II, DP beta 1 (HLA-DPB1) major histocompatibility 9 VOO522 -- complex, class II, DR beta 1 (HLA-DRB1) Major histocompatibility 2 M24O70 complex, class II, Ybox binding protein I; DNA binding protein B (YB1 malate dehydrogenase 1, 1 DSS654 -- NAD (soluble) (mdh1) malate dehydrogenase 1, 3 D55654 NAD (soluble) (MDH1) malonyl-CoA 2 AFO97832 decarboxylase precursor maltase-glucoamylase 1 AFO16833 (mg) manic fringe (Drosophila) 1 U94352 -- homolog (MFNG) mannose phosphate 1 X76057 isomerase (MPI) mannose phosphate 2 X76057 isomerase (mpi) mannose-6-phosphate 3 X56253 receptor (cation dependent) (M6PR) mannose-P-dolichol 1 AFO38961 utilitzation defect 1 (MPDU1) mannosidase, alpha B, 1 U60885 lysosomal (MANB) mannosyl (alpha-1,3-)- 1 MSS621 -- glycoprotein beta-1,2-N- acetylglucosaminyltransferase (MGAT1) map 4q35 repeat region 1 AFO 64849 US 2010/01 24746 A1 May 20, 2010 52

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

MAP kinase-interacting 2 ABOOO409 ------serine/threonine kinase 1 (MKNK1) MAPERK kinase kinase 3 5 UT8876 (MEKK3) MAPERK kinase kinase 5 1 D84476 -- (MEKK5) MAP microtubule affinity 4 M8O359 regulating kinase 3 (MARK3) Marenostrin protein 1 Y14441 MASL.1 1 ABO16816 MAX dimerization protein 3 LO6895 (MAD) MaxiKpotassium channel 1 AFO3SO46 beta subunit MBP-2 for MHC binding 1 X65644 -- protein 2 Meis (mouse) homolog 3 1 U6838S -- (MEIS3) melanoma-associated 1 M12154 antigen p97 (melanotransferrin) membrane cofactor protein 4 X594OS -- (CD46, trophoblast lymphocyte cross-reactive antigen) (MCP) membrane component, 4 D14696 -- -- chromosome 17, Surface marker 2 (ovarian carcinoma antigen CA125) (M17S2) membrane metallo 2 JO3779 B -- -- endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) (MME) membrane protein, 2 M64925 -- -- palmitoylated 1 (55 kD) (MPP1) meningioma expressed 1 U9478O -- antigen (MGEA) meningioma-expressed 1 U73682 ------antigen 11 (MEA11) Menkes Disease (ATP7A) 1 LO6133 putative Cu++-transporting P-type ATPase metallothionein 2A (MT2A) 1 WOOS94 -- -- metaxin 1 (MTX1) 1 U46920 -- methionine 2 X68836 -- -- adenosyltransferase II, alpha (MAT2A) methyl-CpG binding 1 Y10746 domain protein 1 (MBD1) (non-exact 59% aa) methylene tetrahydrofolate 2 X16396 -- -- dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase (MTHFD2) methylenetetrahydrofolate 1 JO4.031 -- -- dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1) methyltransferase, putative 2 AJ224442 US 2010/01 24746 A1 May 20, 2010 53

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene I entification ESTS No. BI Br H K Li Lu HC antigen (HLA-B) U14943 L42024) HCC ass 1 region 2 AFOSSO66 HCC ass I antigen (HLA U70863 ass I antigen (HLA U19736 ass I antigen (HLA U38975 ass I antigen US2813 (HLA-B) ass I antigen HLA-A AFO15930 LA-A) ass I antigen HLA-A U36687 s LA-A-2402 allele) MHC class I antigen HLA X13112 A11K MHC class I antigen HLA-B U67331 (B*0801 variant) (=AF028596) MHC class I antigen HLA-B U67330 (B*0801 variant) (=U88254) MHC class I antigen HLA-B AFO17328 (B*48 allele) MHC class I antigen HLA-B AFO14770 (HLA-B*1502 allele) MHC class I antigen HLA-B US8643 (HLA-B84OMD) MHC class I antigen HLA-B AFO28596 (HLA-B*4103 allele) M ass I antigen HLA-B 9. e le LA-B84402 variant M ass I antigen HLA-B O-B*3910 ass I antigen HLA ass I antigen HLA-A (A*0302) ass I antigen US8469 CHA (HLA-B84403

UO6697 histocompatibility antigen (HLA-B) (clone C21/14) MHC class IHLAB7 LO7950 MHC class IHLA-A Flp (Aw33.1) MHC class IHLA-B U18660 MHC class I HLA-B (HLA U18661 B-07ZEL allele) (=X86704) MHC class I HLA-B (HLA U28759 B-08NR allele) MHC class IHLA-B3512 L76094 MHC class IHLA-B4 U17572 variant (=U17572) MHC class IHLA-B442 M24038 chain MHC class IHLA-B51 L41086 cd3.3 MHC class IHLA-C allele 2 Z33459 MHC class IHLA-CwO304 D641SO (=M84172: M99389) MHC class IHLA-CwO803 3 Z15144 MHC class IHLA-Cw6 M282O6 MHC class I HLA-J antigen LS6139 US 2010/01 24746 A1 May 20, 2010 54

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. O Accession Tissue Distribution

Gene Identification ESTS No. Br H K Li Lu MHC class Ilymphocyte M1967O antigen A2 (A2.1) variant DK1 MHC class Imic-Bantigen X91625 MHC class Ipolypeptide L14848 -- related sequence A (MICA) MHC class I protein HLA-C U61274 heavy chain (C*0701new allele) (=AFO17331) MHC class II DNA Sequence (clone A37G7 1C11) MHC class II DQ-alpha M16995 ------associated with DRw6, DQw1 protein MHC class II DQ-beta M17564 ------associated with DR2, DQw1 protein MHC class II HAL-DQ M33842 LTR5 (DQ, w8) DNA fragment, long terminal MHC class IIhla-dr alpha JOO195 =JOO197; M60334; KO11171; JOO194; M60333; X00274) MHC class IIHLA-DRB1 AFOOf883 MHC class IIHLA-DRw 11 M21966 beta-I chain (DRw11.3) MHC class II lymphocyte M23907 antigen (DPW4-beta-1) MHC CLASS II P33076 RANSACTIVATORCIITA non-exact 57%) HC HLA-E2.1 (=X87679) M32507 HC HLA-E2.1 (alpha-2 M32507 domain) (low match) Mi-2 autoantigen 240 kDa protein (non-exact 84%) microsomal stress 70 UO4735 protein ATPase core (stch) microtubule-associated U19727 ------protein 4 (MAP4) microtubule-associated X73882 protein 7 (MAP7) mineralocorticoid receptor ------(aldosterone receptor) (MLR) minichromosome X62153 ------maintenance deficient (S. cerevisiae) 3 (MCM31) minichromosome ABO11144 ------maintenance deficient (S. cerevisiae) 3-associated protein (MCM3AP) minichromosome X74795 ------maintenance deficient (S. cerevisiae) 5 (cell division cycle 46) (MCM5) mitochondiral cytochromeb AFO42517 (CYTB) mitochondrial 16S rRNA Z70759 mitochondrial ATP XS9066 synthase (F1-ATPase) alpha subunit mitochondrial ATP X69907 synthase c subunit (P1 form) US 2010/01 24746 A1 May 20, 2010 55

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu mitochondrial cytochromeb 6 AFO42508 (CYTB) mitochondrial cytochromeb 1 ABOO62O2 Small subunit of complex II mitochondrial 1 POO395 CYTOCHROMEC OXIDASE POLYPEPTIDE I mitochondrial 1 POO403 CYTOCHROMEC OXIDASE POLYPEPTIDE II mitochondrial cytochrome 2 POO403 C oxidase subunit II mitochondrial cytochrome S U12691 oxidase subunit II (COII) (=U12692 Hsa4 mitochondrion cytochrome oxidase subunit II) mitochondrial DNA loop 1 X89763 attachment sequences (clone LAS34) mitochondrial DNA 1 U94703 polymerase accessory subunit precursor (MtPolB) nuclear gene encoding mitochondrial protein, mitochondrial DNA, 1 X93334 complete genome mitochondrial genes for 8 VOO710 several tRNAs (Phe, Val, Leu) and 12S and 16S ribosomal RNAs. mitochondrial genes for 3 WOO660 tRNA (Phe) and 12S rRNA (fragment) mitochondrial inner 1 AF106622 membrane preprotein translocase Tim17a mitochondrial isolate Afr7 1 AFO42SO3 cytochrome b(CYTB) mitochondrial loop 1 X89843 attachment sequence (clone LAS88) mitochondrial NADH 14 AFO14893 dehydrogenase subunit 2 (ND2) mitochondrial translational 1 L34600 ------initiation factor 2 (MTIF2) mitochondrion cytochromeb 1 UO9SOO mitogen inducible gene 1 Z24725 ------mig-2 mitogen inducible gene 1 Z24725 mig-2 (non-exact, 7.1%) mitogen-activated protein 2 U43784 ------kinase-activated protein kinase 3 (MAPKAPK3) MLNS1 X80199 ------MLN64 (=D38255 CAB1) 1 X801.98 ------moesin (MSN) 14 M69066 ------monocytic leukaemia zinc 2 U47742 ------finger protein (MOZ) MOP1 () 2 U2916S motor protein (Hs.78504) 2 D21094 ------mouse double minute 2, 1 U39736 -- -- human homolog of p53 binding protein (MDM2) M-phase phosphoprotein 6 1 X98263 ------(MPP-6) US 2010/01 24746 A1 May 20, 2010 56

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

M-phase phosphoprotein, X98260 mpp 11 MPS1 L2O314 Mr 110,000 antigen 2 D64154 MRCOX-2, V-like region XOS324 (=M17227) mu-adaptin-related protein YO8387 2; musubunit of AP-4 (MU ARP2) multifunctional polypeptide X53793 -- similar to SAICAR synthetase and AIR carboxylase (ADE2H1) murine leukemia viral (bmi L13689 1) Oncogene homolog (BMI1) mutant (Daudi) beta2 44 XO7621 microglobulin mutated in colorectal 1 M62397 cancers (MCC) myeloid cell leukemia 9 LO8246 -- sequence 1 (BCL2-related) (MCL1) myeloid cell nuclear 11 M817SO -- differentiation antigeN (MNDA) myeloid differentiation 4 U70451 primary response gene (88) (MYD88) myeloid leukemia factor 2 3 U57342 (MLF2) myeloid/lymphoid or mixed 8 U89867 lineage leukemia (trithorax (Drosophila) homolog): translocated to, 7 (MLLT7) MYH9 (cellular myosin 1 M811 OS heavy chain) myomesin (M-protein)2 1 X69089 (165 kD) (MYOM2) myosin IE (MYO1E) 11 X984.11 myosin light chain kinase 1 U48959 -- : (MLCK) myosin phosphatase, 2 D8793O target subunit 1 (MYPT1) myosin regulatory light 2 D50372 chain (=U26162) myosin VIIa (low match 71) 1 US5208 myosin, heavy polypeptide 3 M811 OS -- 9, non-muscle (MYH9) myosin, light polypeptide, 6 XS4304 -- regulatory, non-sarcomeric (20 kD) (MLCB) myosin-I beta 1 X98507 -- myristoylated alanine-rich 1 D10522 : : protein kinase C Substrate (MARCKS, 8OK-L) (MACS) myxovirus (influenza) 1 M3O817 -- resistance 1, homolog of murine (interferon-inducible protein p78) (MX1) myxovirus (influenza) resistance 2, homolog of murine (MX2) N-acetylgalactosaminidase, 2 M62783 alpha-(NAGA) N-acetylglucosamine receptor 1 (thyroid) (NAGR1) US 2010/01 24746 A1 May 20, 2010 57

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

NACP/alpha-synuclein 2 U46896 N-acylaminoacyl-peptide D38441 -- hydrolase (APEH) N-acylsphingosine U47674 -- -- amidohydrolase (acid ceramidase) (ASAH) NAD+-specific isocitrate U49283 -- -- dehydrogenase beta Subunit precursor (encoding mitochondrial protein) NADH dehydrogenase US3468.1 -- -- (ubiquinone) 1 alpha Subcomplex, 5 (13 kD, B13) (NDUFAS) NADH dehydrogenase AFO47181 -- (ubiquinone) 1 beta Subcomplex, 5 (16 kD, SGDH) (NDUFB5) NADH dehydrogenase AFOSO640 -- (ubiquinone) Fe—S protein 2 (49 kD) (NADH-coenzyme Q eductase) (NDUFS2) NADH dehydrogenase M22S38 (ubiquinone) flavoprotein 2 (24 kD) (NDUFV2) NADH:ubiquinone AFOS3070 -- -- dehydrogenase 51 kDa subunit (NDUFV1) NADH-CYTOCHROMEBS POO387 REDUCTASE (BSR) (50% aa) NADH-UBIQUINONE PO3886 OXIDOREDUCTASE CHAIN 1 Nardilysin (N-arginine U64898 ------dibasic convertase) (NRD1) nascent-polypeptide X80909 ------associated complex alpha polypeptide (NACA) natural killer cell group 7 -- -- sequence (NKG7) natural killer cell transcript 19 -- 4 (NK4) natural killer-associated -- blood only transcript 3 (NKAT3) natural killer-associated AFO22045 -- blood only transcript 5 (NKAT5) natural killer-tumor LO4288 B ------recognition sequence (NKTR) N-deacetylase/N- AFO42084 ------Sulfotransferase (heparan glucosaminyl)2 (NDST2) Ndr protein kinase -- Nedd-4-like ubiquitin U961.13 protein ligase WWP1 nel (chicken)-like 2 D83018 -- -- (NELL2) N-ethylmaleimide-sensitive U39412 -- -- factor attachment protein, alpha (NAPA) N-ethylmaleimide-sensitive U78107 ------factor attachment protein, gamma (NAPG) US 2010/01 24746 A1 May 20, 2010 58

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br K Li Lu neural precursor cell 3 X92S44 ------high in testis expressed, developmentally down regulated 5 (NEDD5) neural precursor cell D23662 -- expressed, developmentally down regulated 8 (NEDD8) neuregulin 1 (NRG1) UO2330 neuroblastoma RAS viral 4 ABO2O692 -- : : (v-ras) oncogene homolog (NRAS) Neuroblastoma RAS viral X68286 (v-ras) oncogene homolog (NRAS) (low match) Neurofibromin 2 (bilateral S73853 acoustic neuroma) (NF2) neuronal apoptosis 2 U19251 -- inhibitory protein (NAIP) neuronal cell adhesion ABOO2341 molecule (NRCAM) neuropathy target esterase AJOO4832 (NTE) neuropeptide Y3 receptor, D284.33 5'UTR (low score) neurotrophic tyrosine 14 XO3S41 -- kinase, receptor, type 1 (NTRK1) neutrophil cytosolic factor 4 2 USO720 (40 kD) NG31 AF129756 NGAL (=X83006) X991.33 nibrin (NBS) AFOS1334 NIK ABO14587 Ninjurin 1: nerve injury U72661 induced protein-1 nitrilase 1 (NIT1) AFO6998.7 (=AFO69984) NKG2-D (low match) (non XS4870 exact, 58%) Nmi U32849 N-myristoyltransferase 1 AFO43324 (NMT1) No arches-like (Zebrafish) U79569 Zinc finger protein (NAR) non-histone chromosome DSO42O -- protein 2 (S. cerevisiae)- like 1 (NHP2L1) non-muscle (fibroblast) tropomyosin non-muscle alpha-actinin U48734 non-muscle myosin alkali 3 M22918 -- High in fetal adrenal light chain (Hs.77385) gland and BPH Strona non-neuronal enolase (EC X16289 4.2.1.11) non-receptor tyrosine M33689 phosphatase 1 normal keratinocyte 3 X53778 -- high in many libraries Substraction library mRNA, clone H22a notch group protein (N) M99437 novel protein X9996.1 novel T-cell activation X94232 protein N-ras protein NRU 1 A6O196 US 2010/01 24746 A1 May 20, 2010 59

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI K Li Lu

N-sulfoglucosamine -- Sulfohydrolase (sulfamidase) (SGSH) insulin induced gene 1 1 U96876 -- (INSIG1) integrin, alpha 4 (antigen 3 L12002 -- CD49D, alpha 4subunit of VLA-4 receptor) (ITGA14) interferon, gamma-inducible 1 M63838 -- protein 16 (IFI16) interleukin 1, beta (IL1RB) 1 M15330 nuclear antigen H731-like U83908 protein nuclear antigen Sp100 4 U36SO1 -- (SP100) Nuclear antigen Sp100 1 P23497 (SP100) (85% aa) Nuclear antigen Sp100 1 P23497 (SP100) (89% aa) nuclear autoantigenic 1 M97856 -- sperm protein (histone binding) (NASP) nuclear corepressor KAP-1 1 U78773 (KAP-1) (=U95040; X97548 TIF1beta zinc finger protein) Nuclear domain 10 protein 4 U22897 -- (NDP52) Nuclear factor (erythroid derived 2)-like 2 (NFE2L2) Nuclear factor of kappa 2 MS8603 light polypeptide gene enhancer in B-cells 1 (p105) (NFKB1) nuclear factor of kappa 3 M69043 light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA) nuclear factor related to 1 UO8.191 kappa B binding protein (NFRKB) nuclear mitotic apparatus 3 Z11S83 -- protein 1 (NUMA1) nuclear receptor 1 X97674 coactivator 2 (GRIP1) nuclear receptor 2 AFO10227 -- coactivator 3 (AIB3) nuclear receptor 22 X77548 coactivator 4 (ELE1) nuclear receptor interacting 1 X84373 protein 1 (NRIP1) nuclear respiratory factor 1 1 UO2683 B (NRF1) nuclear RNA helicase, 4 U90426 -- DECD variant of DEAD box family (DDXL) nuclear transcription factor 1 X59711 B Y, alpha (NFYA) nuclear transcription factor, 3 U15306 X-box binding 1 (NFX1) nuclear transport factor 2 1 XO7315 -- (placental protein 15) (PP15) nucleobindin (=M96824) 1 U31336 nucleobindin 1 (NUCB1) M96824 -- nucleolar phosphoprotein 1 Z34289 p130 (P130) US 2010/01 24746 A1 May 20, 2010 60

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu nucleolar protein (KKED 1 ------repeat) (NOP56) nucleolar protein (MSP58) AFO15308 nucleolar protein 1 (120 kD) M32110 -- (NOL1) nucleolar protein p40 nucleolin (NCL) M60858 : : : : nucleophosmin (nucleolar M28699 phosphoprotein B23, numatrin) (NPM1) nucleophosmin-retinoic U41742 acid receptor alpha fusion protein NPM-RAR long form nucleoporin (NUP358) L41840 (=D42063 RanBP2 (Ran binding protein 2)) nucleoporin 153 kD Z25535 (NUP153) nucleoporin 98 kD (NUP98) U41815 nucleosome assembly D28430 protein nucleosome assembly M866.67 protein 1-like 1 (NAP1L1) nucleosome assembly U77456 protein 1-like 4 (NAP1L4) nucleosome assembly D28430 protein, 5'UTR olfactory receptor (OR7 U86281 141) OLFACTORY RECEPTOR P34982 LIKE PROTEIN HGMPO7E (OR17-4) (non-exact 65%) oligodendrocyte myelin LO5367 glycoprotein (OMG) O-linked N U77413 acetylglucosamine (GlcNAc) transferase (UDP-N- acetylglucosamine:polypeptide N-acetylglucosaminyl transferase) (OGT) oncofetal trophoblast AS3531 glycoprotein 5T4 precursor (non-exact 55%) Oncogene TIM (TIM) (non exact 84%) ORF (Hs.77868) M68864 -- ORF1; MER37; putative U49973 transposase similar to pogo element Length = 454 origin recognition complex, U27459 Subunit 2 (yeast homolog)- like (ORC2L) origin recognition complex, AFO22108 Subunit 4 (yeast homolog)- like (ORC4L) (low match) ornithine aminotransferase M23204 (gyrate atrophy) (OAT) ornithine decarboxylase (ODC) ornithine decarboxylase D78.361 -- -- High in pancreas, antizyme, ORF1 and ORF2 and activated T cells orphan receptor UO7132 -- -- (Hs.100221) OS-9 precurosor ABOO2806 -- US 2010/01 24746 A1 May 20, 2010 61

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu osteonectin (=X82259 BM 1 D28381 40) ovel centrosomal protein 1 ABOO8515 -- RanBPM (RANBPM) over-expressed breast 1 L34839 tumor protein Oviductal glycoprotein 1, 1 UO9550 -- -- 120 kD (OVGP1) oxidase (cytochrome c) 1 X80695 -- -- assembly 1-like (OXAIL) oxoglutarate 4 D10523 T -- dehydrogenase (lipoamide) (OGDH) oxysterol binding protein M86917 -- -- (OSBP) OZF X70394 -- OZF (non-exact zinc finger) X70394 p21 iCdc42, Rac1-activated 2 US 1120 -- -- kinase 1 (yeast Ste20 related) (PAK1) P35-related protein (= D63392 S80990 ficolin) 340 U93569 p40phox (=U50720) X77094 P47 LBC oncogene 4 UO3634 p53-induced protein AFO10315 -- -- (PIG11) p54.nrb (low match) Y11287 p62 nucleoporin X58521 b63 mRNA for X6991O -- -- transmembrane protein PAC clone DJO701O16 Q07108 rom 7q33-q36 (non-exact 54%) palmitoyl-protein 10 U44772 -- hioesterase (ceroid ipofuscinosis, neuronal 1, infantile; Haltia-Santavuori disease) (PPT) papillary renal cell 1 X9972O ------carcinoma (translocation associated) (PRCC) PAR protein 1 AF115850 -- partial EST (clone c-1gh O4) 1 Z43627 PAX3 forkhead 1 UO2368 transcription factor gene usion paxillin (PXN) D86862 -- PBK1 protein AJOO7398 -- : : -- : PBS-EST (nz92e01.s1 AA732534 NCI CGAP GCB1 clone MAGE: 1302936) (low score) PDZ domain protein 1 AJ224747 -- -- (Drosophila inaD-like) (INALD) PEBP2aCRunt domain 1 Z38108 encoding gene (=Z35728) peptidase D (PEPD) 1 JO4605 peptidylprolyl isomerase A 3 YOOO52 ------high in many libraries (cyclophilin A) (PPIA) peptidylprolyl isomerase D 2 L11667 T -- (cyclophilin D) (PPID) peptidylprolyl isomerase E 1 AFO42386 -- (cyclophilin E) (PPIE) PERB11.1 (=U56942 MHC 1 U69630 class I chain-related protein A) US 2010/01 24746 A1 May 20, 2010 62

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu perforin 1 (preforming 14 M28393 protein) (PRF1) peroxisomal acyl-CoA 2 X86032 hioesterase (PTE 1) Peroxisomal acyl X71440 coenzyme A oxidase peroxisomal fairnesylated X75535 protein (PXF) phorbol-12-myristate-13 D9007O B, W acetate-induced protein (PMAIP1) phosphate carrier X77337 (mitochondrial gene'?) Phosphate carrier, 3 X60O36 ------

L28957 -- -- cytidylyltransferase 1, choline, alpha isoform (PCYT1A) PHOSPHATIDATE Q92903 CYTIDYLYLTRANSFERASE (CDP-DIGLYCERIDE) phosphatidylinositol 3 2 US 7843

Subuni phosphatidylinositol 4 3 ABOOS910 ------kinase, catalytic, beta polypeptide (PIK4CB) hosphatidylinositol glycan, 1 L19783 ass H (PIGH) hosphatidylinosito 2 D30O37 transfer protein (PI-TPbeta) hosphatidylinosito 2 X98.654 B, T ansfer protein, lymphoma membrane-associated (PITPNM) phosphatidylinosito X98.654 transfer protein, membrane-associated (PITPNM) (non-exact 64%) phosphatidylinositol-4- U14957 -- -- phosphate 5-kinase, type , alpha (PIP5K2A) phatihatidylinositol-4- U85.245 ------hate 5-kinase, type , beta (PIPSK2B) phosphodiesterase 7A L12052 B, W -- -- (PDE7A) phosphodiesterase IB U56976 ONLY (PDES1B) phosphoglucomutase 1 2 M83O88 ------( PGM1) hosphogluconate U3O255 -- ehydrogenase (PGD) hosphoglycerate kinase 1 12 VOO572 (PGK1) phosphoglycerate mutase 3 JO4173 1 (brain) (PGAM1) phosphoglycerate mutase 1 M55673 -- -- 2 (muscle) (PGAM2) phosphoinositide-3-kinase, 1 Z29090 -- -- catalytic, alpha polypeptide (PIK3CA) phosphoinositide-3-kinase, 4 U86453 ------catalytic, delta polypeptide (PIK3CD) US 2010/01 24746 A1 May 20, 2010 63

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI K Li Lu phosphoinositide-3-kinase, 1 X83368 catalytic, gamma polypeptide (PIK3CG) phospholipase C 1 X14034 phospholipase C, delta 1 2 UO9117 (PLCD1) phospholipase C, gamma 1 1 M34667 -- (formerly subtype 148) (PLCG1) phospholipid scramblase 1 AFOO8445 phosphoribosyl 1 D61391 pyrophosphate synthetase associated protein 1 (PRPSAP1) phosphoribosylglycinamide 3 XS41.99 ormyltransferase, phosphoribosylglycinamide synthetase, hosphoribosylaminoimidazole ynthetase (GART) phosphorylase kinase, 3 D38616 alpha 2 (liver), glycogen storage disease IX (PHKA2) phosphorylase, glycogen; 1 U47O2S -- b rain (PYGB) hosphorylase, glycogen; 1 U47O2S rain (PYGB) (low match, l on-exact, 75%) hosphorylase, glycogen; 1 Y15233 iver (Hers disease, ycogen storage disease type VI) (PYGL) phosphorylation regulatory 2 protein HP-10 phosphotidylinositol D3.0036 -- transfer protein (PITPN) pigment epithelium-derived U29953 -- actor (PEDF) pim-1 oncogene (PIM1) M24779 -- pinin, desmosome U77718 B, associated protein (PNN) monocyte, T lymphoma acenta (Diff33) S U491.88 acenta (Diff33) (non U491.88 exact, 69%) acenta (Diff48) 18 U491.87 -- acenta (Diff48) (low U491.87 match) placenta (Diff48) (low U491.87 match) plasminogen activator, X74039 urokinase receptor (PLAUR) platelet factor 4 (PF4) M25897 plateletendothelial cell 8 M37780 : : adhesion molecule (CD31 ntigen) (PECAM1) platelet-activating factor 4 U89386 acetylhydrolase 2 (40 kD) (PAFAH2) atelet-activating factor 1 U72342 -- 8. cetylhydrolase, isoform lb., alpha subunit (45 kD) ( PAFAH1B1) atelet-activating factor 1 D102O2 receptor (PTAFR) pleckstrin (PLEK) 10 XO7743 US 2010/01 24746 A1 May 20, 2010 64

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu pleckstrin (PLEK) (low 1 XO7743 match) pleckstrin homology, Sect 4 M85169 -- -- and coiled coil domains 1(cytohesin 1) (PSCD1) pleckstrin homology, Sect 4 LO6633 -- -- and coiled coil domains, binding protein (PSCDBP) pM5 protein 1 X57398 -- -- PMP69 2 Y14322 poly (ADP-ribose) 1 X56140 polymerase (NAD(+) ADP ribosyltransferase) (=X16674) poly(A) polymerase (PAP) 1 X76770 ------poly(A)-binding protein-like 19 YOO345 ------1 (PABPL1) poly(rC)-binding protein 1 3 X78137 ------(PCBP1) polyadenylate binding 1 U75686 protein polycystic kidney disease 1 S U24498 (autosomal dominant) (PKD1) polymerase (DNA 1 D29013 -- directed), beta (POLB) polymerase (DNA 6 D84103 directed), gamma (POLG) polymerase (RNA) II (DNA 1 X63564 ------directed) polypeptide A (220 kD) (POLR2A) polymyositis, scleroderma 1 LO1457 ------autoantigen 2 (100 kD) (PMSCL2) polypyrimidine tract binding 1 X65372 ------protein (heterogeneous nuclear ribonucleoprotein I) (PTB) positive regulator of 3 U13021 programmed cell death CH-1L (Ich-1) postmeiotic segregation 1 M16514 -- -- increased 2-like 12 (PMS2L12) postmeiotic segregation 1 U38964 -- -- increased 2-like 8 (PMS2L8) potassium inwardly 1 D87291 -- rectifying channel, Subfamily J, member 15 (KCNJ15) potassium voltage-gated 1 AFOS1426 -- channel, KQT-like Subfamily, member 1 (KCNQ1) POU domain, class 2, 1 Z491.94 -- associating factor 1 (POU2AF1) POU domain, class 2, 2 X13403 -- transcription factor 1 (POU2F1) PPAR binding protein 1 Y13467 -- -- (PPARBP) PPARgamma2 1 D83233 pre-B-cell colony 8 UO2O20 enhancing factor (PBEF) prefoldin 1 (PFDN1) 1 Y17392 ------prefoldin 5 (PRFLD5) 3 D89667 B : : -- US 2010/01 24746 A1 May 20, 2010 65

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu prefoldin subunit 3 1 Y17394 (=U96759 von Hippel Lindau binding protein (VBP-1)) pregnancy-associated 1 U28727 -- -- high in placenta plasma protein A (PAPPA) pre-mRNA splicing factor 1 UO881S ------SF3a (60 kD), similar to S. cerevisiae PRP9 (spliceosome-associated protein 61) (SF3A60) pre-mRNA splicing factor 1 UO881S SF3a (60 kD), similar to S. cerevisiae PRP9 (spliceosome-associated protein 61) (SF3A60) (low score) pre-mRNA splicing factor 2 D28423 SRp20, 5'UTR preprotein translocase 3 X97544 ------(TIM17) prion protein 1 X82S45 prion protein (p27-30) 1 M13899 ------(Creutzfeld-Jakob disease, Gerstmann-Strausler Scheinker syndrome, fatal amilial insomnia) (PRNP) pristanoyl-CoA oxidase 1 Y11411 (low match) pristanoyl-CoA oxidase 1 Y11411 (low score) rocollagen-lysine, 2- 1 M982S2 ------Oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehlers Danlos syndrome type VI) (PLOD) procollagen-proline, 2- 1 M24486 ------oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide 1

rocollagen-proline, 2- 4 XOS130 ------xoglutarate 4-dioxygenase proline 4-hydroxylase), eta polypeptide (protein isulfide isomerase; thyroid hormone binding protein p55) (P4HB) profilin 1 (PFN1) 1 JO3.191 ------progesterone receptor- 2 U28918 -- associated p48 protein (P48) prohibitin (PHB) 1 S85655 ------proliferating cell nuclear 3 JO4718 ------antigen (PCNA) proliferation-associated 4 L1918.4 ------gene A (natural iller enhancing factor A) (PAGA) proline-rich protein BstNI 1 S62936 Subfamily 2 (PRB2) (non exact, 43% aa) proline-serine-threonine 1 U94778 phosphatase interacting protein 1 (PSTPIP1) prolyl endopeptidase 2 X74496 ------(PREP) US 2010/01 24746 A1 May 20, 2010 66

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu prolylcarboxypeptidase 5 L13977 ------(angiotensinase C) (PRCP) promyelocytic leukemia 1 M80185 ------(PML) properdin P factor, 4 XS7748 -- complement (PFC) pro-platelet basic protein 1 M54995 ------(includes platelet basic protein, beta hromboglobulin, connective issue-activating peptide III, neutrophil-activating peptide-2) (PPBP) pro-platelet basic protein 7 M54995 ------(includes platelet basic protein, beta hromboglobulin, connective tissue-activating peptide III, neutrophil activating peptide-2) (PPBP) proprotein convertase 4 U40623 Subtilisin?kexin type 7 (PCSK7) prosaposin (variant 89 DOO422 ------Gaucher disease and variant metachromatic eukodystrophy) (PSAP) prostaglandin 1 U63846 ------endoperoxide synthase 1 (prostaglandin GH synthase and cyclooxygenase) (PTGS1) prostaglandin 2 L15326 endoperoxide synthase 2 (prostaglandin GH synthase and cyclooxygenase) (PTGS2) prostaglandin 1 D64O68 endoperoxide synthase-1 (=L08404: U84208) (all promoters) prostate carcinoma tumor 2 L78132 antigen (pcta-1) protease inhibitor 1 (anti 17 KO2212 ------high in many libraries elastase), alpha-1- antitrypsin (PI) protease inhibitor 2 (anti 1 M930S6 -- -- elastase), monocytefneutrophil (ELANH2) (low match) proteasome prosome, 3 LO2426 B ------macropain) 26S subunit, ATPase, 1 (PSMC1) proteasome prosome, 1 M34O79 ------macropain) 26S subunit, ATPase, 3 (PSMC3) proteasome prosome, 2 AFO2O736 macropain) 26S subunit, ATPase, 4 (PSMC4) proteasome prosome, S L38810 ------macropain) 26S subunit, ATPase, 5 (PSMC5) proteasome prosome, 2 D78275 ------macropain) 26S subunit, ATPase, 6 (PMSC6) US 2010/01 24746 A1 May 20, 2010 67

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI H K Li Lu proteasome prosome, 1 AFOO1212 T -- macropain) 26S subunit, non-ATPase, 11 (PSMD11) proteasome prosome, 2 D781.51 -- -- macropain) 26S subunit, non-ATPase, 2 (PSMD2) proteasome prosome, 1 S79862 T -- -- macropain) 26S subunit, non-ATPase, 5 (PSMD5) proteasome prosome, 1 DSOO63 ------high in many libraries macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) (PMSD7) proteasome prosome, 1 ABOO3103 ------macropain) 26S subunit, on-ATPase, 12 (PMSD12) proteasome prosome, 3 LO7633 ------macropain) activator subunit 1 (PA28 alpha) proteasome prosome, 2 DOO762 ------macropain) subunit, alpha type, 3 (PSMA3) proteasome prosome, 3 X61970 ------macropain) subunit, alpha type, 5 (PSMA5) proteasome prosome, 3 AFOS418S macropain) subunit, alpha proteasome prosome, 1 AFO22815 macropain) subunit, alpha type, 7 (PSMA7) (low l proteasome prosome, 1 DOO761 -- macropain) subunit, beta proteasome prosome, 1 X71874 ------macropain) subunit, beta type, 10 (PSMB10) proteasome prosome, 1 D29012 ------macropain) subunit, beta proteasome prosome, 1 U17497 ------macropain) subunit, beta type, 8 (large multifunctional protease 7) (PSMB8) proteasome (prosome, 3 Z14977 ------macropain) subunit, beta type, 9 (large multifunctional protease 2) (PSMB9) proteasome (prosome, 1 D38048 -- macropain) subunit, beta type, 7 (PSMB7) protective protein for beta 3 M22960 -- galactosidase (galactosialidosis) (PPGB) protein A alternatively 1 U47925 spliced form 2 (A-2) protein activator of the 1 AFO72860 high in testis interferon-induced protein kinase (PACT) protein disulfide isomerase 2 D494.89 -- related protein (P5) US 2010/01 24746 A1 May 20, 2010 68

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu protein 1 L25441 ------geranylgeranyltransferase type I, beta subunit (PGGT1B) protein homologous to 20 M24.194 ------+ high in many libraries chicken B complex protein, guanine nucleotide binding (H12.3) protein kinase Aanchoring 1 AFO37439 -- protein protein kinase C substrate 2 USO317 ------8OK-H (PRKCSH) protein kinase C, beta 1 6 XO6318 ------(PRKCB1) protein kinase C, delta 1 D10495 ------(PRKCD) protein kinase C, eta 1 MSS284 -- -- (PRKCH) protein kinase C, mu 1 X75756 (PRKCM) (non-exact 78%) Protein kinase C-like 1 2 D26181 ------(PRKCL1) protein kinase, AMP- 1 U42412 B, T -- -- activated, gamma 1 non- lymphoma catalytic subunit (PRKAG1) protein kinase, cAMP- 4 M18468 ------dependent, regulatory, type , alpha (tissue specific extinguisher 1) (PRKAR1A) protein kinase, DNA- 1 U47077 ------activated, catalytic polypeptide (PRKDC) protein kinase, mitogen- 1 Z11695 B -- -- activated 1 (MAP kinase 1: p40, p41) (PRKM1) protein kinase, mitogen- 1 L77964 ------activated 6 (extracellular signal-regulated kinase, p97) (PRKM6) protein kinase, mitogen- 1 U66839 ------activated, kinase 3 (MAP kinase kinase 3) (PRKMK3) protein phosphatase 1, S. M63960 ------catalytic Subunit, alpha isoform (PPP1CA) protein phosphatase 1, 3 Y13247 ------regulatory Subunit 10 (PPPR10) protein phosphatase 1, 2 ZSO749 ------regulatory Subunit 7 (PPP1R7) protein phosphatase 2 1 X12656 ------(formerly 2A), catalytic subunit, beta isoform (PPP2CB) protein phosphatase 2 1 LO7590 ------(formerly 2A), regulatory subunit B" (PR 72), alpha isoform and (PR130), beta isoform (PPP2R3) protein phosphatase 2, 2 L42373 ------regulatory subunit B (B56), alpha isoform (PPP2R5A) protein phosphatase 2, 3 D7836O ------regulatory subunit B (B56), delta isoform (PPP2R5D) US 2010/01 24746 A1 May 20, 2010 69

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI H K Li Lu protein phosphatase 2, 1 D26445 ------regulatory subunit B (B56), gamma isoform (PPP2R5C) protein phosphatase 2A S. JO2902 ------regulatory Subunit alpha isotype (alpha-PR65) protein phosphatase 4 2 AFO97996 ------(formerly X), catalytic subunit (PPP4C) protein tyrosine kinase 2 4 L492O7 -- -- beta (PTK2B) protein tyrosine 1 XS4134 phosphatase epsilon protein tyrosine 2 L48723 ------phosphatase type IVA, member 2 (PTP4A2) protein tyrosine 1 M31724 ------phosphatase, non-receptor ype 1 (PTPN1) protein tyrosine 1 M93425 high in testis phosphatase, non-receptor ype 12 (PTPN12) protein tyrosine 1 M93425 pnosphatase, non-receptor type 12 (PTPN12) (non , 70%) in tyrosine 2 M2S393 ------natase, non-receptor PTPN2) in tyrosine 1 M68941 ------natase, non-receptor e (megakaryocyte) N4) e in tyrosine 7 M74903 ------phatase, non-receptor e 6 (PTPN6) R. ein tyrosine 1 D11327 ------OO natase, non-receptor (PTPN7) R. ein tyrosine 1 M34668 ------sphatase, receptor alpha polypeptide RA) t in tyrosine 44 YOO638 ------phatase, receptor c polypeptide RC) O: in tyrosine 1 X58288 ------hosphatase, receptor type, M (PTPRM) protein tyrosine 2 U81561 -- -- phosphatase, receptor type, N polypeptide 2 (PTPRN2) protein with polyglutamine 1 U94836 ------repeat (ERPROT213-21) protein-kinase, interferon 1 U28424 inducible double stranded RNA dependent inhibitor (PRKRI) protein-L-isoaspartate (D- 4 D13892 aspartate) O methyltransferase (PCMT1) proteoglycan 1, secretory 7 JO3223 granule (PRG1) prothymosin, alpha (gene 12 M14483 -- sequence 28) (PTMA) US 2010/01 24746 A1 May 20, 2010 70

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu prp.28, U5 snRNP 100 kod 7 AFO26402 ------protein (U5-10OK) PRP4/STK/WD splicing 1 AFOO1687 -- actor (HPRP4P) PTK7 protein tyrosine 1 U4O271 kinase 7 (PTK7) purinergic receptor P2X, 3 AFOOO234 igand-gated ion channel, 4 (P2RX4) purinergic receptor P2X, Y12851 -- macrophage only igand-gated ion channel, 7 (P2RX7) puromycin-sensitive YO7701 aminopeptidase (PSA) putative ATP(GTP)-binding 2 AJO10842 protein putative brain nuclearly ABO183O8 - argeted protein (KIAAO765) putative chemokine D10923 -- receptor; GTP-binding protein (HM74) putative dienoyl-CoA AFO3O249 isomerase (ECH1) putative G-binding protein AFO65393 Putative human HLA class U73477 B I associated protein I (PHAP1) Putative L-type neutral ABOO7896 amino acid transporter (KIAAO436) putative mitochondrial AFOSO198 space protein 32.1 PUTATIVEMUCIN CORE Q04900 PROTEIN PRECURSOR 24 (MULTI GLYCOSYLATED CORE PROTEIN 24) (MGC-24) (MUC-24) putative nucleic acid 2 X763O2 -- binding protein putative outer 1 US8970 mitochondrial membrane 34 kDa translocase Htom34 putative p150 (non-exact 1 U93568 88%) putative translation 1 L26247 -- -- High in moderately initiation factor (SUI1) differentiated colon adenocarcinoma putative tumor Suppressor 1 AFO61836 protein (123F2) pyrroline 5-carboxylate 1 M77836 -- reductase pyruvate dehydrogenase 1 D90O84 (lipoamide) alpha 1 (PDHA1) pyruvate dehydrogenase 2 JO3576 -- (lipoamide) beta (PDHB) Pyruvate dehydrogenase 3 Y1314.5 complex, lipoyl-containing component X; E3-binding protein (PDX1) pyruvate kinase, muscle 11 M23725 (PKM2) RAB, member of RAS 1 U1842O oncogene family-like (RABL) US 2010/01 24746 A1 May 20, 2010 71

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

RA B1, member RAS 3 M28209 ------ogene family (RAB1) B11A, member RAS 2 XS6740 ------high in spleen ogene family (RAB11A) B11B, member RAS D45418 -- ogene family (Rab11B) B27A, member RAS U38654 -- ogene family (RAB27A) B5B, member RAS XS4871 ------ogene family (RAB5B) B6, member RAS M28.212 -- ogene family (RAB6) B7, member RAS X934.99 ------ogene family (RAB7) B7, member RAS D84488 ------ogene family-like 1 (RAB7L1) RAB9, member RAS U44103 oncogene family (RAB9) RAD50 (S. cerevisiae) U63139 -- -- homolog (RAD50) RAD51 (S. cerevisiae) AFO29669 ------homolog C (RAD51C) Radin blood group (RD) LO3411 ------RAE1 (RNA export 1, U8472O -- : ------S. pombe) homolog (RAE1) ralA-binding protein L42542 ------(RLIP76) RAN binding protein 2-like (RANBP2L1) Ran GTPase activating X82260 ------protein 1 (RANGAP1) RAN, member RAS M31469 oncogene family (RAN) (low match) RanBP2 (Ran-binding D42063 protein 2) (=U19248: L41840 sapiens nucleoporin (NUP358)) ransforming growth factor, DSO683 ------beta receptor II (70-80 kD) (TGFBR2) RAP1A, member of RAS 10 M22995 -- oncogene family (RAP1A) RAR-related orphan U16997 receptor C (RORC) RAS guanyl releasing Y12336 protein 2 (calcium and DAG-regulated) ras homolog gene family, 12 XOSO26 ------high in ovary member A (ARHA) ras homolog gene family, X61587 -- -- member G (rho G) (ARHG) ras homolog gene family, Z35227 ------member H (ARHH) ras inhibitor (RIN1) M37191 Ras-GTPase activating AFO53535 ------protein SH3 domain binding protein 2 (KIAAO660) Ras-GTPase-activating U3.2519 ------protein SH3-domain binding protein (G3BP) ras-related C3botulinum 11 M29871 -- -- toxin substrate 2 (rho family, Small GTP binding protein Rac2) (RAC2) US 2010/01 24746 A1 May 20, 2010 72

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

RAS-RELATED PROTEIN 1 PO9526 RAP-1B (GTP-BINDING PROTEIN SMG P21B) RBQ 1 X851.33 -- -- rearranged T cell receptor 1 LO6891 beta variable region (TCRB) (=X58810) regulator of Fas-induced 1 AFO57557 B -- apoptosis (TOSO) regulator of G protein 1 AFO7392O signalling 6 (RGS6) regulator of G-protein 2 AFO37195 ------signalling 14 (RGS14) regulator of G-protein 6 L13391 ------signalling 2, 24 kD (RGS2) regulator of G-protein 1 O15539 signalling 5 (RGS5) (49% aa) regulatory factor X, 4 1 M69297 -- -- (influences HLA class II expression) (RFX4) regulatory factor X, 5 2 X85786 T -- -- (influences HLA class II expression (RFX5) replication protein A1 1 M63488 ------(RPA1) replication protein A3 1 LO7493 (14 kD) (RPA3) (low match) reproduction 8 (D8S2298E) 1 D83767 -- -- requiem, apoptosis 2 U94585 -- : ------response Zinc finger gene requiem,(REQ) apoptosis 1 U94585 response Zinc finger gene (REQ) (=AFOO1433) (low match) restin (Reed-Steinberg cell 1 M975O1 B, T -- expressed intermediate filament-associated protein) (RSN) retinoblastoma 1 (including 3 L11910 ------osteosarcoma) (RB1) retinoblastoma binding AFO87481 protein 2 homolog 1 (RBBP2H1) retinoblastoma-binding S66427 -- protein 1 (RBBP1) retinoblastoma-binding S S66431 ------protein 2 (RBBP2) retinoblastoma-binding X71810 ------protein 4 (RBBP4) retinoblastoma-binding X74262 ------protein 4 (RBBP4) retinoblastoma-binding U3S143 protein 7 (RBBP7) retinoblastoma-like 2 X76061 ------(p130) (RBL2) retinoic acid receptor AFO)6O228 ------responder (tazarotenenduced) 3 (RARRES3) retinoic acid receptor, XO6538 -- -- alpha (RARA) retinoic acid responsive USO383 -- -- (NN8-4AG) retinoid X receptor beta 2 X66424 ------(RXR-beta) US 2010/01 24746 A1 May 20, 2010 73

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu REV3 (yeast homolog)-like, 1 AFO35537 catalytic subunit of DNA polymerase Zeta (REV3L) Rho GDP dissociation 23 LO7916 inhibitor (GDI) beta (ARHGDIB) Rho GTPase activating 2 X78817 protein 4 (ARHGAP4) Rho GTPase activating 1 P98.171 protein 4 (ARHGAP4) (low match) Rho-associated, coiled-coil 1 ABO14519 containing protein kinase 2 (ROCK2) ribonuclease 6 precursor 2 U85625 (RNASE6PL) ribonuclease 6 precursor 1 U85625 (RNASE6PL) (low match) ribonuclease, RNase A 1 XSS988 family, 2 (liver, eosinophil derived neurotoxin) (RNASE2) ribonucleasef angiogenin 3 M36717 inhibitor (RNH) ribonucleoside diphosphate 1 X65708 reductase M1 subunit ribonucleotide reductase 1 P31350 M2 polypeptide (non-exact 91%) ribophorin I (RPN1) 1 YOO281 ------ribophorin II (RPN2) 1 YOO282 ribosomal 18S rRNA 3 M10098 ribosomal 28S RNA 1 M11167 ribosomal phosphoprotein 1 D28418 P0, 5'UTR (low match) Ribosomal protein 1 ribosomal protein L10 3 O L25899 hig h in many libraries (RPL10) RIBOSOMAL PROTEIN 2 P53O25 L10A (CSA-19) ribosomal protein L11 4 X79234 Alveolar (RPL11) rha bdomyosarcoma ribosomal protein L12 2 LO6SOS (RPL19) ribosomal protein L13 1 P26373 hig h in many libraries (PRL13) ribosomal protein L14 4 D87735 hig h in many libraries (RPL14) ribosomal protein L17 4 X53777 blood only (RPL17) ribosomal protein L18 10 L11566 (RPL18) ribosomal protein L183 S LOSO93 High in fetal adrenal (RPL18A) gland and skin ribosomal protein L183 2 X80821 homologue ribosomal protein L19 15 X63527 (RPL19) ribosomal protein L21 6 U14967 (RPL21) ribosomal protein L22 3 D17652 (RPL22) ribosomal protein L23 2 X55954 hig h in many libraries (RPL23) ribosomal protein L23a 5 U37230 hig h in many libraries (RPL23A) ribosomal protein L26 8 X69392 (RPL26) US 2010/01 24746 A1 May 20, 2010 74

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu ribosomal protein L27 6 LOSO94 ------(RPL27) ribosomal protein L27a 10 U14968 ------(RPL27A) ribosomal protein L28 6 U14969 ------(RPL28) ribosomal protein L29 6 U10248 ------(RPL29) ribosomal protein L3 81 ------+ high in many libraries (RPL3) ribosomal protein L3 81 XO6323 homologue ribosomal protein L30 6 X79238 ------+ high in lymphoma (RPL30) ribosomal protein L30 1 X79238 (RPL30) (low score) ribosomal protein L31 10 X15940 ------+ High in alveolar (RPL31) rhabdomyosarcoma ribosomal protein L32 3 XO3342 ------(RPL32) ribosomal protein L33-like 1 AFO47440 ------(RPL33L) ribosomal protein L34 S L38941 ------(RPL34) ribosomal protein L34 1 L38941 (RPL34) (low match) ribosomal protein L37 S D23661 ------+ high in barstead (RPL37) OState ribosomal protein L37a 4 X66699 ------+ high in many libraries ribosomal protein L38 1 Z26876 ------+ high in many libraries (PRL38) ribosomal protein L4 27 D23660 ------+ high in many libraries (RPL4) ribosomal protein L41 4 AFO26844 ------+ high in many libraries (RPL41) ribosomal protein L5 14 U14966 ------+ High in alveolar (RPL5) rhabdomyosarcoma ribosomal protein L5 1 U14966 (RPL5) (low match) ribosomal protein L6 7 X69391 ------+ high in many libraries (RPL6) ribosomal protein L7 14 XS2967 ------+ high in conorm (RPL7) ribosomal protein L7a 1S M36O72 ------+ High in uterus, and (RPL7A) Seminoma ribosomal protein L8 S Z284O7 ------+ high in ovary (RPL8) ribosomal protein L9 10 UO9953 ------(RPL9) ribosomal protein S10 5 U14972 ------+ high in many libraries (RPS10) ribosomal protein S11 4 XO6617 ------+ high in many libraries (RPS11) ribosomal protein S11 1 ABOO71S2 (RPS11) (low match) ribosomal protein S12 3 X53505 ------+ high in many libraries (RPS12) ribosomal protein S13 2 LO1124 ------(RPS13) ribosomal protein S14 12 M13934 ------(RPS14) ribosomal protein S15 2 M324OS ------(RPS15) ribosomal protein S16 3 M60854 ------+ High in prostate (RPS16) invasive tumor ribosomal protein S17 2 M13932 ------+ high in many libraries (RPS17) ribosomal protein S18 8 X691SO US 2010/01 24746 A1 May 20, 2010

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. K Li ri bosomal protein S19 7 M81757 high in many libraries (RPS19) ri bosomal protein S2 4 high in many libraries (RPS2) RIBOSOMAL PROTEINS2 P1588O (RPS4) ri bosomal protein S20 LO6498 high in many libraries (RPS20) ri bosomal protein S21 LO4483 high in CD34+/CD38 (RPS21) hematopoietic cells and skin tumor ri bosomal protein S23 D14530 (RPS23) ri bosomal protein S24 high in uterus (RPS24) ri bosomal protein S25 M64716 high in barstead (RPS25) OState ri bosomal protein S26 X69654 (RPS26) ri bosomal protein S27 U57847 ((metallopainstimulin 1) (RPS27) ri bosomal protein S28 US8682 (RPS28) ri bosomal protein S29 U14973 (RPS29) ri bosomal protein S3 X55715 high in many libraries (RPS3) ri bosomal protein S3 U14990 (RPS3) (low match) ri bosomal protein S3A Z83334 high in many libraries (RPS3A) ri bosomal protein S3A M77234 (RPS3A) (low score) ri bosomal protein S4, X MS8458 high in ovary and linked (RPS4X) Synovial sarcoma ri bosomal protein S4, Y MS8459 linked (RPS4Y) ri bosomal protein S5 U14970 high in lymphoma (RPS5) RIBOSOMAL PROTEIN S6 P10660 (PHOSPHOPROTEIN NP33) ri bosomal protein S6 22 (RPS6) ri bosomal protein S6 M77232 ( RPS6)) ((non-exact 86% ) bosomal protein S6 LO7597 kinase, 90 kD, polypeptide (RPS6KA1) bosomal protein S6 X85106 kinase, 90 kD, polypeptide

bosomal protein S7 Z25749

bosomal protein S8 X67247

bosomal protein S9 U14971 colon tumor

bosomal protein, large, PO 18 M1788S bosomal protein, large, P1 12 M17886

ibosomal RNA 18S 11 (=M10098; KO3432) =polyadenylating Sequence) ibosomal RNA28S M11167 US 2010/01 24746 A1 May 20, 2010 76

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu ribosomal RNA, 16S U25.123 ring finger protein (non AJOO1019 exact 58%) ring finger protein 3 (RNF3) AJOO1019 ring finger protein 4 (RNF4) 3 ABOOO468 ring zinc-finger protein 3 U41315 : : : : (ZNF127-Xp) RNA (guanine-7-) ABOO7858 methyltransferase (RNMT) RNA binding motif protein 5 4 U23946 -- (RBMS) RNA binding motif, single D28483 stranded interacting protein 2 (RBMS2) RNA helicase (putative), X98743 -- (Myc-regulated DEAD box protein) (MRD8) RNA helicase-related AFO832SS protein RNA pol II largest subunit 2 X74872 RNA polymerase I subunit AFOO8442 (RPA40) RTVP-1 protein 2 X91911 ------S100 calcium-binding 2 M81457 protein A10 (annexin II igand, calpactin I, light polypeptide (p11)) (S100A10) S100 calcium-binding protein A11 (calgizZarin) (S100A11) S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)(S100A4) S100 calcium-binding 7 M21 OOS high in bone marrow protein A8 (calgranulin A) (S100A8) S100 calcium-binding 14 XO6233 high in invasive protein A9 (calgranulin B) larynx squamous cell (S100A9) carcinoma S164 gene 1 AF109907 S-adenosylmethionine 3 M880O3 -- decarboxylase 1 (AMD1) SB classII 5 M27487 -- histocompatibility antigen alpha-chain SC35-interacting protein 1 S AFO3O234 -- (SRRP129) scaffold attachment factor 1 UT2355 -- B (SAFB) scaffold attachment factor 1 UT2355 B (SAFB) (non-exact 78%) scRNA molecule, 1 L13713 transcribed from Alu repeat SEC14 (S. cerevisiae)-like 4 D67029 (SEC14L) SEC23-like protein B 2 X97O65 -- (SEC23B) SEC63 (SEC63) 1 AF1 OO141 Secreted protein, acidic, M2S746 high in bone marrow cysteine-rich (osteonectin) Strona (SPARC) Secretory carrier 1 AFO38966 membrane protein 1 (SCAMP1) US 2010/01 24746 A1 May 20, 2010 77

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

Secretory carrier 1 AFOOSO38 ------membrane protein 2 (SCAMP2) Secretory carrier AFOOSO39 membrane protein 3 (SCAMP3) Secretory granule M33649 proteoglycan core (clones lambda-PG|6,7,8) Selectin L (lymphocyte 43 X17519 -- adhesion molecule 1) (SELL) selectin P ligand (SELPLG) UO2297 -- Sema domain, U60800 : immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D) SeriArg-related nuclear AFO48977 matrix protein (plenty of prolines 101-like) (SRM160) serine palmitoyltransferase YO8685 ------subunit I (SPTI) serine palmitoyltransferase, ABO11098 ------subunit II (LCB2) serine protease JO2907 serine protease inhibitor, U78095 ------Kunitz type, 2 (SPINT2) serine/threonine kinase 10 ABO15718 ------(STK10) serine/threonine kinase 19 L26260 ------(STK19) serine/threonine kinase 4 U18297 -- (STK4) serine/threonine protein X66358 ------kinase KKLALRE (KKIALRE) serine/threonine protein -- -- kinase (NIK) SERINETHREONINE P37023 PROTEINKINASE RECEPTORR3 PRECURSOR (SKR3) Serologically defined colon FO39694 cancer antigen 16 (NY-CO 16) Serologically defined colon FO396.98 B, T -- -- cancer antigen 33 (SDCCAG33) Serologically defined colon FO396.98 cancer antigen 33 (SDCCAG33) (low score) Serologically defined colon FO396.98 cancer antigen 33 (SDCCAG33) (low score) serum deprivation FO85481.1 response (phosphatidylserine-binding protein) (SDPR) (=S67386) serum glucocorticoid Y OO32 ------regulated kinase (SGK) SET domain, bifurcated 1 D31891 ------(SETDB1) US 2010/01 24746 A1 May 20, 2010 78

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI H K Li Lu

S H2 domain protein 1A, 1 AFO73019 T -- Duncan's disease lymphoproliferative syndrome) (SH2D1A) S H3 binding protein (SAB) 2 ABOOSO47 - S H3 domain protein 1B 4 U61167 -- : : H3BGR PROTEIN (-21 1 P55822 GLUTAMIC ACID-RICH P ROTEIN; 21-GARP) (non exact 82% aa) S H3-binding domain 1 AFO42O81 -- 9. utamic acid-rich protein li ke (SH3BGRL) S H3-domain GRB2-like 1 1 U65999 --

HC (Src homology 2 2 X68148 domain-containing) transforming protein 1 (SHC1) siah binding protein 1 2 US 1586 (SiahBP1) siah binding protein 1 1 US 1586 (SiahBP1) (non-exact, 69%) Sialomucin CD164 9 D14043 (CD164) sialophorin (gpL115, 2 JO4536 leukosialin, CD43) (SNP) sialyltransferase (STHM) U145SO ------sialyltransferase 1 (beta 2 X17247 -- galactoside alpha-2,6- sialytransferase) (SIAT1) sialyltransferase 4A (beta AFOS9321 B galactosidase alpha-2,3- sialytransferase) (SIAT4A) sialyltransferase 8 (alpha L41680 ,8-polysialytransferase) D (SIAT8D) signal peptidase 25kDa. Subunit signal recognition particle X73459 -- 14 kD (homologous Alu RNA-binding protein) (SRP14) signal recognition particle US 1920 54 kD (SRP54) signal recognition particle 2 U20998 9 signal recognition particle 5 XO6272 receptor (docking protein) signal regulatory protein, 5 Y10376 beta, 1 (SIRP-BETA-1) signal sequence receptor, 2 Z12830 alpha (translocon associated protein alpha) signal sequence receptor, 2 X74104 -- beta (translocon associated protein beta) signal transducer and 4 L41142 -- activator of transcription (STATSA) signal transducer and 1 U18671 activator of transcription 2, 113 KD (STAT2) US 2010/01 24746 A1 May 20, 2010 79

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu signal transducer and 3 L29277 activator of transcription 3 (acute-phase response factor) (STAT3) signal transducer and 2 U4873O -- activator of transcription 5A (STATSA) signal transducing adaptor 1 U43899 molecule (SH3 domain and ITAM motif) 1 (STAM) silencing mediator of 1 U37146 retinoid and thyroid hormone action (SMRT) similar to beta-transducin 1 UO2609 ------Superfamily proteins (SAZD) similar to S. cerevisiae 1 ABO11169 ------SSM4 (TEB4) similar to yeast pre-mRNA 1 AFO26031 ------splicing factors, Prp1 Zer1 and Prp6 SIT protein 1 AJO10059.1 Sjogren syndrome antigen 2 M62800 -- A1 (52 kD, ribonucleoprotein autoantigen SS-A/Ro) (SSA1) Sjogren syndrome antigen 1 M62800 A1 (52 kD, ribonucleoprotein autoantigen SS-A/Ro) (SSA1) (non-exact 63%) (match to Zinc finger) SKAP55 homologue 1 AJOO4886 ------(SKAP-HOM) skb1 (S. pombe) homolog 2 AFO15913 ------(SKB1) skeletal muscle abundant 1 X87613 ------protein SMA3 (SMA3) 1 X833OO ------Small acidic protein 3 U51678 ------Small EDRK-rich factor 2 2 Y10351 ------high in fetal lung (SERF2) Small inducible cytokine A5 2 M21121 -- high in many libraries (RANTES) (SCYA5) Small inducible cytokine 1 D63789 Subfamily C, member 2 (SCYC2) Small nuclear 2 M15841 ribonucleoprotein polypeptide B" (SNRPB2) Small nuclear 4 JO4615 -- ribonucleoprotein polypeptide N (SNRPN) Small nuclear 2 JO4564 -- ribonucleoprotein polypeptides B and B1 (SNRPB) Small nuclear RNA 1 AFO93593 -- activating complex, polypeptide 5, 19 kD (SNAPC5) Smallest subunit of 1 DSS636 -- high in fetal lung ubiquinol-cytochrome c reductase SMC (mouse) homolog, X 1 L2S270 -- chromosome (SMCX) SMT3B protein (2) 2 X99585 -- US 2010/01 24746 A1 May 20, 2010 80

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu SNARE protein (YKT6) (low 1 U95735 match) SNC19 1 U20428 SNC73 protein (SNC73) 2 JOO220 -- -- high in many libraries solute carrier family 1 2 U53347 : : : (neutral amino acid transporter), member 5 (SLC1A5) Solute carrier family 11 7 DSO403 -- (proton-coupled divalent metal ion transporters), member 1 (SLC11A1) solute carrier family 17 1 U90545 -- (sodium phosphate), member 3 (SLC17A3) solute carrier family 19 1 U17566 B, -- -- (folate transporter), lymphoma member 1 (SLC19A1) solute carrier family 2 1 KO3195 ------(facilitated glucose transporter), member 1 (SLC2A1) solute carrier family 23 3 D87075 ------(nucleobase transporters), member 2 (SLC23A2) solute carrier family 25 1 AFO70548 B, T ------(mitochondrial carrier; oxoglutarate carrier), member 11 (SLC25A11) solute carrier family 31 3 U.83461 -- -- (copper transporters), member 2 (SLC31A2) Solute carrier family 4, 1 X621.37 ------anion exchanger, member 2 (erythrocyte membrane protein band 3-like 1) Solute carrier family 4, 1 ABO18282 -- Sodium bicarbonate cotransporter, member 8 (SLC4A8) solute carrier family 7 2 M8O244 T, W ------(cationic amino acid transporter, y+ system), member 5 (SLC7A5) solute carrier family 7 3 D87432 ------(cationic amino acid transporter, y+ system), member 6 (SLC7A6) solute carrier family 7 1 D87432 (cationic amino acid transporter, y+ system), member 6 (SLC7A6) (non exact 77%) solute carrier family 9 1 AFO3O409 ------(sodium hydrogen exchanger), isoform 6 Somatic cytochrome c 2 M22877 (HCS) SON DNA binding protein 2 X63753 ------(SON) Son of sevenless 1 L13858 ------(Drosophila) homolog 1 (SOS1) sorcin (SRI) 1 M32886 sortilin 1 (SORT1) 2 X98248 ------US 2010/01 24746 A1 May 20, 2010 81

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu sortilin-related receptor, 6 YO8110 L(DLR class) A repeats containing (SORL1) sorting nexin 1 (SNX1) 3 U53225 ------sorting nexin 2 (SNX2) 2 AFO43453 sorting nexin 6 (SNX6) 1 AF121856.1 (=U83194.1 TRAF4 associated factor 2) Sp3 transcription factor 1 X68560 ------(SP3) Sp3 transcription factor 4 M97191 ------(SP3) special AT-rich sequence 1 M97287 binding protein 1 (binds to nuclear matrix scaffold associating DNAs) (SATB1) speckle-type POZ protein 4 AJOOO644 (SPOP) speckle-type POZ protein 1 AJOOO644 (SPOP) (non-exact) spectrin SH3 domain 6 U87166 ------binding protein 1 (SSH3BP1) Spectrin, alpha, non 2 JOS243 ------erythrocytic 1 (alpha-fodrin) (SPTAN1) spermidine?spermine N1 11 M55580 acetyltransferase (SAT) spermidine?spermine N1 1 U40369 acetyltransferase (SAT) (non-exact, 84%) spermine synthase (SMS) 1 ADOO1528 ------SPF31 (SPF31) 1 AFO831.90 ------sphingomyelin 1 X52679 ------phosphodiesterase 1, acid lysosomal (acid phingomyelinase) SMPD1) PINDLINEHOMOLOG 1 Q99865 PROTEIN DXF34) pinocerebellar ataxia 1 3 X792.04 B -- -- O ivopontocerebellar ataxia , autosomal dominant, axin 1) (SCA1) pinocerebellar ataxia 2 1 UTO323 B -- O ivopontocerebellar ataxia , autosomal dominant, axin 2) (SCA2) pi l O C erebellar ataxia 7 2 AJOOOS17 -- ivopontocerebellar trophy with retinal egeneration) (SCA7) Spliceosome associated 3 U41371 ------protein (SAP 145) plicing factor (CC1.3) 2 L10910 ------CC1.3) icing factor SRp40-1 7 U3O826 ------S Rp40) plicing factor, 3 M740O2 B ------arginine serine-rich 11 (SFRS11) splicing factor, 4 L41887 ------arginine serine-rich 7 (35 kD) (SFRS7) Src-like adapter protein 1 U3O473 (non-exact, 76% aa) Src-like-adapter (SLA) 6 D89077 ------US 2010/01 24746 A1 May 20, 2010 82

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu Src-like-adapter (SLA) (low D89077 match) Src-like-adapter (SLA) (low U44403 score) stannin (SNN) 2 AFO3O196 ------STAT induced STAT ABOO4904 -- inhibitor 3 (SSI-3) STE20-like kinase 3 (MST 2 AFO246.36 ------3) step II splicing factor SLU7 AF101.074 ------(SLU7) steroid sulfatase M17591 steroid sulfatase JO4964 -- -- (microsomal), arylsulfatase C, isozyme S (STS) sterol carrier protein 2 MSS421 (SCP2) sterol O-acyltransferase AFOS92O2 (acyl-Coenzyme A: cholesterol acyltransferase) 1 (SOAT1) stimulated trans-acting 6 X82200 -- factor (50 kDa) (STAF50) Striatin, calmodulin-binding 1 U17989 protein (STRN) (low match,

Stromal antigen 2 (STAG2) 2 Z75331 ------stromal interaction 3 US2426 ------molecule 1 (STIM1) structure Specific 1 M86737 ------recognition protein 1 (SSRP1) Succinate dehydrogenase S L21936 -- complex, Subunit A, flavoprotein (Fp) (SDHA) Succinate dehydrogenase 1 U17248 ------complex, Subunit B, iron sulfur (Ip) (SDHB) Succinate dehydrogenase 1 US 7877 ------complex, Subunit C, integral membrane protein, 15 kD (SDHC) Succinate dehydrogenase 3 ABOO62O2 -- -- complex, Subunit D, Integral membrane protein (SDHD) Succinate-CoA ligase, 1 AFOS8954 GDP-forming, beta subunit (SUCLG2) Succinyl CoA synthetase 1 Z68204 SudD (Suppressor of AFO13591 -- -- bim)6, Aspergilius nidulans) homolog (SUDD) Sulfotransferase family 1A, 1 L19999 -- -- phenol-preferring, member 1 (SULT1A1) Sulfotransferase family 1A, 1 U376.86 phenol-preferring, member 3 (SULT1A3) (non-exact 67%) Superoxide dismutase 1, 4 XO2317 ------Soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1) Superoxide dismutase 2, 5 YOO985 mitochondrial (SOD2) Supervillin (SVIL) 2 AFOS1851 Suppression of 2 U15131 -- -- tumorigenicity 5 (ST5) US 2010/01 24746 A1 May 20, 2010 83

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu

Suppression of 1 U15779 tumorigenicity 5 (ST5) (non-exact 82%) Suppressor of K+ transport 1 AFO38960 -- -- defect 1 (SKD1) Suppressor of Ty 1 AFO64804 + ------(S. cerevisiae) 3 homolog (SUPT3H) Suppressor of Ty 2 U38817 ------(S. cerevisiae) 4 homolog 1 (SUPT4H1) Suppressor of Ty 2 US 64O2 (S. cerevisiae) 5 homolog (SUPTSH) Suppressor of Ty 2 U46691 -- (S. cerevisiae) 6 homolog (SUPT6H) Suppressor of variegation 1 AFO19968 3-9 (Drosophila) homolog 1 (SUV39H1) Survival of motor neuron 1, 1 U18423 telomeric (SMN1) SWI/SNF related, matrix 1 M881 63 associated, actin dependent regulator o chromatin, Subfamily a, member 1 (SMARCA1) (non-exact, 75%) SWI/SNF related, matrix 2 D2615S associated, actin dependent regulator o chromatin, Subfamily a, member 2 (SMARCA2) SWI/SNF related, matrix 1 D261.56 -- associated, actin dependent regulator o chromatin, Subfamily a, member 4 (SMARCA4) SWI/SNF related, matrix 4 U66616 -- associated, actin dependent regulator o chromatin, Subfamily c, member 2 (SMARCC2) SWI/SNF related, matrix 2 AFO35262 B, W ------associated, actin dependent regulator o chromatin, Subfamily e, member 1 (SMARCE1) synaptobrevin-like 1 1 X958O3 ------(SYBL1) synaptosomal-associated 2 AJO11915 ------protein, 23 kD (SNAP23) Syndecan binding protein 15 AFOO6636 ------(syntenin) (SDCBP) synovial sarcoma, 2 X792O1 translocated to X chromosome (SSXT) Syntaxin 16 1 AFO38.897 Syntaxin 3A (STX3A) 2 U32315 -- -- Syntaxin 6 (STX6) 1 AJOO2O78.1 SYNTAXIN BINDING 1 OOO186 PROTEIN3 (UNC-18 HOMOLOG3) (UNC-18C) Syntaxin-16C 1 AFOO8937 SYT interacting protein 1 AFO80S61 ------(SIP) T cell activation, increased 4 M88.282 -- late expression (TACTILE) US 2010/01 24746 A1 May 20, 2010 84

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu T cell receptor Valpha 2 XS8744 gene segmentV-alpha-7 (clone IGRa11) T cell receptor Valpha 1 X5874O gene segmentV-alpha-w27 T3 receptor-associating S S83390 ------cofactor-1 tafazzin (cardiomyopathy, 1 X92763 ------dilated 3A (X-linked): endocardial fibroelastosis 2: Barth syndrome) (TAZ) TAFII100 protein (non 1 U8O191 exact 53%) tankyrase, TRF1 1 AFO82556 ------interacting ankyrin-related ADP-ribose polymerase (TNKS) TAP1, TAP2, LMP2, LMP7 1 X664O1 and DOB TAR DNA-binding protein 6 U23731 ------43 at interactive protein 2 U4O989 ------(60 kD) (TIP60) TATA box binding protein 1 OOO268 (TBP)-associated factor, RNA polymerase II, C1, 30 kD (TAF2C1) (non exact, 55%) TATA box binding protein 4 X97999 ------(TBP)-associated factor, RNA polymerase II, F, 5 kD (TAF2F) TATA box binding protein 2 U21858 ------( B C ) -associated factor, RNA polymerase II, G, 2 kD (TAF2G) TATA box binding protein D63705 ------(TBP)-associated factor, RNA polymerase II, I, 28 kD (TAF2I) ax1 (human T-ce U33821 ------eukemia virus type I) binding protein 1 (TAX1BP1) T-box 2 (TBX2) (non-exact U28049 ------77%) TBP-associated factor 172 AJOO1017 ------TAF-172) (T-cell death-associated U95218 -- ene 8 (TDAG8) T-cell leukemia/lymphoma X82240 -- A (TCL1A) T-cell leukemia, lymphoma X82240 A (TCL1A) (low match) T-cell receptor (delta D2 M22197 1-region) (clone K3B) T-cell receptor (V beta 5.1, M97705 beta 1.5, C beta1) (low match) T-cell receptor alpha delta 2 AEOOO662 (=M94081) T-cell receptor alpha 1 B3962S enhancer-binding protein, short form (=X58636 Mouse LEF1 lymphoid enhancer binding factor 1 (=D16503)) US 2010/01 24746 A1 May 20, 2010 85

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu T-cell receptor delta gene 1 M22197 D2-J1-region, clone K3B T-cell receptor germline 1 M11955 beta chain gene V-region (V)V-beta-MT1-1 T-cell receptor germline 1 M14159 -- only in blood beta-chain gene J2.1 exon T-cell receptor germline 2 M221S2 elta-chain D-J region T-cell receptor interacting 2 AJ224878 -- molecule (TRIM) protein T-cell receptor rearranged 1 M21784 elta-chain, V-region (V- elta 3-J) T-cell receptor, alpha 3 AEOOO660 ------(V, D, J, C) (TCRA) T-cell receptor, beta cluster 3 L34740 ------+ high in pancreas (TCRB) T-cell receptor, delta 2 X73617 ------(V, D, J, C) (TCRD) T-cell, immune regulator 1 3 U4528S only found in tumor (TCIRG1) TCF-1 mRNA for T cell X59870 factor TCF-1 mRNA for T cell X59870 factor 1 (splice form B) (low match) T-COMPLEX PROTEIN 1, Q99832 ETA SUBUNIT (TCP-1- ETA) (CCT-ETA) (HIV-1 NEF INTERACTING PROTEIN) T-COMPLEX PROTEIN 1, P50990 THETA SUBUNIT (TCP-1- THETA) (CCTTHETA) (KIAAO002) TCR eta = T cell S94421 receptor(eta-exon) TCRW Beta 13.2 X75419 TERA ACOO4472 estis enhanced gene 33 X75861 ------transcript (TEGT) etracycline transporter-like 2 L11669 ------protein (TETRAN) etratricopeptide repeat U46570 ------domain 1 (TTC1) etratricopeptide repeat U46571 ------domain 2 (TTC2) etratricopeptide repeat D84296 ------domain 3 (TTC3) TGFB1-induced anti- D86970 ------apoptotic factor 1 (TIAF1) hioredoxin reductase 1 3 S79851 ------(TXNRD1) THIOREDOXIN- P3004.8 DEPENDENT PEROXIDE REDUCTASE PRECURSOR, mitochondrial (ANTI OXIDANT PROTEIN 1) (AOP-1) hreonyl-tRNA synthetase 1 M6318O ------(TARS) hrombin inhibitor 1 Z22658 hrombospondin 1 (THBS1) 2 XO4665 ------hromboxane A synthase 1 1 M80647 ------(platelet, cytochrome P450, subfamily V) (TBXAZ1) US 2010/01 24746 A1 May 20, 2010 86

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu hymidine kinase 2, 2 X76104 -- -- mitochondrial (TK2) hymidylate kinase (CDC8) 1 L16991 ------hymine-DNA glycosylase US 1166 ------(TDG) Thymosin, beta 10 2 M2O259 -- (TMSB10) hymosin, beta 4, X 29 M17733 ------chromosome (TMSB4X) hyroid autoantigen 70 kD 7 JO4611 (Kuantigen) (G22P1) hyroid hormone receptor 1 AFO16270 -- -- coactivating protein (SMAP) hyroid hormone receptor 2 L40357 ------interactor 7 (TRIP7) hyroid hormone receptor 4 L40411 interactor 8r (TRIP8) hyroid hormone receptor D83.783 associated protein, 230 kDa subunit (TRAP230) hyroid receptor interacting 2 L40388 ------protein 15 (TRIP15) TI-227H D50525 TIA1 cytotoxic granule M77142 ------associated RNA-binding protein (TIA1) issue inhibitor of XO2598 -- metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) (TIMP1) issue inhibitor of M32304 -- high in placenta metalloproteinase 2 (TIMP2) issue specific US8766 -- transplantation antigen P35B (TSTA3) itin (TTN) X64697 ------high in muscle TNF receptor-associated U12597 : ------actor 2 (TRAF2) TNF receptor-associated AF1109081 actor 3 (TRAF3) TNF receptor-associated U78798 actor 6 (TRAF6) (low match) ol-like receptor 1 (TLR1) U88540 -- ol-like receptor 2 (TLR2) U88878 ------ol-like receptor 4 (TLR4) U8888O -- ol-like receptor 5 (TILR5) -- opoisomerase (DNA) I -- -- (TOP1) opoisomerase (DNA) II ------beta (180 kD) (TOP2B) opoisomerase (DNA) III 3 D87012 -- beta (TOP3B) TR3beta 1 D85245 -- TRAF family member 3 U63830 -- associated NF-kB activator (TANK) TRANSALDOLASE 1 P37837 transaldolase 1 (TALDO1) L19437 transaldolase-related 1 AFO10398 protein transcobalamin II (TCII) 1 AFO47576 transcription elongation 2 Z47087 -- factor B (SIII), polypeptide 1-like (TCEB1L) US 2010/01 24746 A1 May 20, 2010 87

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI K Li Lu transcription elongation 1 L47345 ------actor B (SIII), polypeptide 3 (110 kD, elongin A) (TCEB3) transcription factor 12 1 M83233 -- (HTF4, helix-loop-helix transcription factors 4) (TCF12) transcription factor 17 2 D89928 (TCF17 transcription factor 4 2 X52079 (TCR4) transcription factor 6-like 1 2 M62810 -- (mitochondrial transcription actor 1-like) (TCF6L1) transcription factor 7-like 2 Y11306 (T-cell specific, HMG-box) (TCF7L2) transcription factor binding X96717 -- o IGHM enhancer 3 (TFE3 transcription factor IL-4 7 AFO67575 -- Stat transcription factor IL-4 U16031 Stat (low match) transcription factor ISGF-3 4 M97935 (=M97936) transcription factor REST AS6138 transcription factor TFIID Z22828 transcriptional adaptor 2 AFO64094 (ADA2, yeast, homolog)- ike (TADA2L) transcriptional intermediary AFOO9353 actor 1 (TIF1) (non-exact 72%) transducin (beta)-like 1 Y12781 -- (TBL1) transducin-like enhancer of M994.38 -- split 3, homolog of Drosophila E(sp1) (TLE3) Transformation transcription AFO76974 -- domain-associated protein (TRRAP) transformation-sensitive, 2 M86752 similar to Saccharomyces cerevisiae STI1 (STI1L) transforming growth factor 1 ABOO9356 beta-activated kinase 1 (TAK1) (non-exact 78%) transforming growth factor 3 AJ2227OO -- beta-stimulated protein TSC-22 (TSC22) transforming growth factor, 1 LO7594 beta receptor III (betaglycan, 300 kD) (TGFBR3) transforming growth factor, 2 4507466 -- beta-induced, 68kD (TGFBI) TRANSFORMING 2 Q15582 GROWTH FACTOR-BETA NDUCED PROTEINIG-H3 PRECURSOR (BETA IG H3) transforming, acidic coiled 1 AFO4991O coil containing protein 1 (TACC1) (non-exact 70%) transgelin 2 (TAGLN2) 14 D21261 -- US 2010/01 24746 A1 May 20, 2010 88

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu transgelin 2 (TAGLN2) D21261 (non-exact) trans-Golgi network protein 2 AFO29316 (46,48, 51 kD isoforms) (TGN51) transient receptor potential X89066 channel 1 (TRPC1) transketolase (Wernicke 7 L12711 Korsakoff syndrome) (TKT) translation factor Sui1 AFO 646O7 homolog (GC20) translin (TSN) 3 X78627 -- translin-associated factor X X95073 : : : : (TSNAX) transmembrane U79725 glycoprotein (A33) transmembrane protein X6991O -- (63 kD), endoplasmic reticulum Golgi intermediate compartment (P63) transmembrane protein 1 1 ABOO1523 (TMEM2) TRANSMEMBRANE 1 P51805 PROTEINSEX PRECURSOR (non-exact 65%) transmembrane trafficking 2 X97442 -- protein (TMP21) transporter 1, ABC (ATP 3 L21208 -- binding cassette) (TAP1) Treacher Collins 2 U40847 -- -- high in many libraries Franceschetti syndrome 1 (TCOF1) triosephosphate isomerase 2 X69723 -- (TPI1) tropomyosin tropomyosin 4 (TPM4) : : : : : TRPM-2 protein 2 M63376 tryptase I precursor (non A35863 exact 64%)(=P20231) tryptophan rich basic Y12478 protein (WRB) tryptophanyl-tRNA XS9892 -- synthetase (WARS) Ts translation elongation L37936 -- actor, mitochondrial (TSFM) topoisomerase (DNA) II Z15115 beta (180 kD) Tu translation elongation 4 L3899S actor, mitochondrial (TUFM) tuberous Sclerosis 1 AFO131.68 (TSC1) tuberous Sclerosis 2 X75621 (TSC2) tubulin, alpha 1 (testis XO6956 specific) (TUBA1) tubulin, alpha, ubiquitous 11 KOOSS8 -- -- high in many libraries (K-ALPHA-1) tubulin, alpha, ubiquitous KOOSS8 (K-ALPHA-1) (low match) tubulin-specific chaperone U61234 c (TBCC) tumor necrosis factor 7 U37518 (ligand) Superfamily, member 10 (TNFSF10) US 2010/01 24746 A1 May 20, 2010

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu tumor necrosis factor 1 AFO46888 ------(ligand) Superfamily, member 13 (TNFSF13) tumor necrosis factor 1 AFO36581 (ligand) Superfamily, member 14 (TNFSF14) tumor necrosis factor 1 D38122 -- Found only in library (ligand) Superfamily, 386: T-cell lymphoma member 6 (TNFSF6) tumor necrosis factor 1 LO9753 B only (ligand) Superfamily, member 8 (TNFSF8) tumor necrosis factor 1 AFO 61034 ------alpha-inducible cellular protein containing leucine Zipper domains (FIP2) Tumor necrosis factor 2 M63928 -- -- receptor Superfamily member 7 (TNFRSF7) tumor necrosis factor 1 AFO16266 ------receptor Superfamily, member 10b (TNFRSF10B) tumor necrosis factor 3 AFO12629 -- receptor Superfamily, member 10c, decoy without an intracellular domain (TNFRSF10C) tumor necrosis factor 1 AFO23849 found only in prostate receptor Superfamily, member 10d, decoy with truncated death domain (TNFRSF1OD) (non-exact 84%) tumor necrosis factor 1 U945.08 ------receptor Superfamily, member 12 (translocating chain-association membrane protein) (TNFRSF12) tumor necrosis factor 1 UTO321 ------receptor Superfamily, member 14 (herpesvirus entry mediator) (TNFRSF14) tumor necrosis factor 5 US 21.65 ------receptor Superfamily, member 1B (TNFRSF1B) tumor necrosis factor 1 X63717 B, W -- receptor Superfamily, member 6 (TNFRSF6) tumor necrosis factor 1 M63928 -- -- receptor Superfamily, member 7 (TNFRSF7) tumor necrosis factor, 8 M92357 ------alpha-induced protein 2 (TNFAIP2) tumor necrosis factor, 2 M59465 alpha-induced protein 3 (TNFAIP3) tumor protein 53-binding 1 AFO78776 ------protein, 1 (TP53BP1) tumor protein p53 (Li 1 M14695 ------Fraumeni syndrome) (TP53) Tumor protein p53-binding 1 U82939 ------protein (TP53BPL) US 2010/01 24746 A1 May 20, 2010 90

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

of Accession Tissue Distribution

Gene Identification ESTS No. BI K Li Lu tumor protein, 3S X16064 translationally-controlled 1 (TPT1) tumor protein, X16064 translationally-controlled 1 (TPT1) (low score) tumor rejection antigen X15187 -- (gp96) 1 (TRA1) tumorous imaginal discs AFO61749 (Drosophila) homolog (TID1) TXK tyrosine kinase (TXK) L27071 type II integral membrane AJOO1685 found only in fetal protein (NKG2-E) liverispleen TYRO protein tyrosine AFO 19562 kinase binding protein (TYROBP) tyrosine 3 XS7346 -- -- high in ecnorm monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB) tyrosine 3 M86400 monooxygenase/tryptophan 5-monooxygenase activation protein, Zeta polypeptide (YWHAZ) tyrosine 3 M86400 monooxygenase/tryptophan 5-monooxygenase activation protein, Zeta polypeptide (YWHAZ) Tyrosine kinase 2 (TYK2) XS4637 TYROSINE-PROTEIN P43403 KINASEZAP-70 (70 KD ZETA-ASSOCIATED PROTEIN) (SYK-RELATED TYROSINE KINASE) tyrosyl-tRNA synthetase U89436 -- (YARS) O1 small nuclear RNA M14387 U19H snoRNA (=M63485 AJ224166 R. norvegicus matrin 3) U2(RNU2) Small nuclear M96982 RNA auxillary factor 1 (non standard symbol) (U2AF1) U22 snoRNA host gene (UHG) O4/U6-associated RNA AFO16370 splicing factor (HPRP3P) O49 small nuclear RNA X96649 U5 SnRNP-specific protein ABOO7510 - (220 kD), ortholog of S. cerevisiae Prp8p (PRP8) U5 SnRNP-specific protein, D21163 -- 16 kD (U5-116 KD) U5 SnRNP-specific protein, Z70200 200 kDa (DEXH RNA helicase family) (U5-200-KD) Uba80 mRNA for ubiquitin S79522 -- hig h in ovary ubiquinol-cytochrome c DSS636 -- hig h in fetal lung reductase (6.4 kD) subunit (UQCR) US 2010/01 24746 A1 May 20, 2010 91

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI Br H K Li Lu UBIQUINOL 1 P47985 CYTOCHROMEC REDUCTASE IRON SULFUR SUBUNIT PRECURSOR (RIESKE RON-SULFUR PROTEIN) (RISP) (low match) ubiquitin A-52 residue 2 XS 6999 ribosomal protein fusion product 1 (UBA52) ubiquitin activating enzyme 1 AFO94516 -- -- E1-like protein (GSA7) ubiquitin C (UBC) S ABOO9010 ------high in ovary ubiquitin carboxyl-terminal 1 M3O496 -- : ------esterase L3 (ubiquitin hiolesterase) (UCHL3) ubiquitin fusion degradation 1 U64444 ------like (UFD1L) ubiquitin protein ligase E3A 1 U84404 B -- -- (human papilloma virus E6 associated protein, Angelman syndrome) (UBE3A) biquitin Specific protease 4 D80012 ------0 (USP10) biquitin Specific protease U44839 -- 1 (USP11) biquitin Specific protease 3 ABO11101 ------5 (USP15) biquitin Specific protease ABO2O698 9 (USP19) biquitin Specific protease AFO1730S B ------(proto-oncogene) (USP4) biquitin Specific protease AFO17306 (proto-oncogene) (USP4) non-exact, 66%) biquitin Specific protease Z724.99 ------(herpesvirus-associated) P7) biquitin Specific protease 5 D29956 ------(USP8) BIQUITIN-ACTIVATING P22314 YME E1 (A1S9 OTEIN) (56%) biquitin-activating enzyme MS8028 ------1 (A1S9T and BN75 temperature sensitivity complementing) (UBE 1) ubiquitin-activating enzyme L341.70 ------E1, like (UBE1L) UBIQUITIN-BINDING U41806 -- -- PROTEIN P62; phosphotyrosine independent ligand for the Lck SH2 domain pé2 (P62) ubiquitin-conjugating 2 U49278 -- enzyme E2 variant 1 (UBE2V1) ubiquitin-conjugating 1 X98091 enzyme E2 variant 2 (UBE2V2) UBIQUITIN 1 Q16781 CONUGATING ENZYME E2-17 KD (UBIQUITIN PROTEIN LIGASE) ubiquitin-conjugating 1 M74525 -- enzyme E2B (RAD6 homolog) (UBE2B) US 2010/01 24746 A1 May 20, 2010 92

TABLE 2-continued Comparison of 1,800 Unique Genes Identified in the Blood Cell cDNA Library to Genes Previously Identified in Specific Tissues

No. of Accession Tissue Distribution

Gene Identification ESTS No. BI H K Li Lu ubiquitin-conjugating 1 AFO32456 ------enzyme E2G 2 (homologous to yeast UBC7) (UBE2G2) ubiquitin-conjugating Z29328 -- enzyme E2H (homologous to yeast UBC8) (UBE2H) ubiquitin-conjugating X92962 enzyme E2L 1 (UBE2IL1) ubiquitin-conjugating AJOOOS 19 enzyme E2L 3 (UBE2IL3) ubiquitin-conjugating AFO31141 enzyme E2L6 (UBE2IL6) ubiquitin-like 1 (Sentrin) U61397 -- (UBL1) UDP-N-acetyl-alpha-D- galactosamine:polypeptide N acetylgalactosaminyltransferase 2 (GalNAc-T2) (GALNT2) UDP-N-acetyl-alpha-D- X92689 galactosamine:polypeptide N acetylgalactosaminyltransferase 3 (GalNAc-T3) (GALNT3) (non-exact 65%) unactive progesterone L24804 receptor, 23 Kod (P23) unconventional myosin-ID U57053 (MYO1F) uncoupling protein U94592 homolog (UCPH) uncoupling protein U94592 homolog (UCPH) (low match 67%) Unknown gene product ACOO2310 unknown mRNA (clone AFO70542 24514) unknown protein (clone Z70223 ICRFp507L0677) unknown protein AFO70626 -- s.93832) nknown protein IT14 ppressor of Ty D79984 -- cerevisiae) 6 homolog pregulated by 1.25 74 S73591 -- -- high in heart ihydroxyvitamin D-3 VDUP1) pregulated by 1.25 S73591 di hydroxyvitamin D-3 (VDUP1) (low match) upregulated by 1,25 S73591 dihydroxyvitamin D-3 (VDUP1) (low match) upregulated by 1,25 S73591 dihydroxyvitamin D-3 (VDUP1) (low score) upstream binding factor XS3461 -- (hUBF) UV radiation resistance associated gene (UVRAG) vacuolar proton-ATPase, X71490 subunit D: V-ATPase, subunit D (ATP6DV) v-akt murine thymoma viral M631.67 -- oncogene homolog 1 (AKT1)