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2018 Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion Margot L. K. Williams Washington University School of Medicine in St. Louis

Atsushi Sawada Washington University School of Medicine in St. Louis

Terin Budine Washington University School of Medicine in St. Louis

Chunyue Yin Vanderbilt University

Paul Gontarz Washington University School of Medicine in St. Louis

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Recommended Citation Williams, Margot L. K.; Sawada, Atsushi; Budine, Terin; Yin, Chunyue; Gontarz, Paul; and Solnica-Krezel, Lilianna, ,"Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion genes." Nature communications.9,1. 1319. (2018). https://digitalcommons.wustl.edu/open_access_pubs/7584

This Open Access Publication is brought to you for free and open access by Digital Commons@Becker. It has been accepted for inclusion in Open Access Publications by an authorized administrator of Digital Commons@Becker. For more information, please contact [email protected]. Authors Margot L. K. Williams, Atsushi Sawada, Terin Budine, Chunyue Yin, Paul Gontarz, and Lilianna Solnica- Krezel

This open access publication is available at Digital Commons@Becker: https://digitalcommons.wustl.edu/open_access_pubs/7584 ARTICLE

DOI: 10.1038/s41467-018-03715-w OPEN Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion genes

Margot L.K. Williams1, Atsushi Sawada1,2, Terin Budine1, Chunyue Yin2,3, Paul Gontarz1 & Lilianna Solnica-Krezel1,2

1234567890():,; Anteroposterior (AP) axis extension during gastrulation requires embryonic patterning and morphogenesis to be spatiotemporally coordinated, but the underlying genetic mechanisms remain poorly understood. Here we define a role for the conserved chromatin factor Gon4l, encoded by ugly duckling (udu), in coordinating tissue patterning and axis extension during zebrafish gastrulation through direct positive and negative regulation of expression. Although identified as a recessive enhancer of impaired axis extension in planar cell polarity (PCP) mutants, udu functions in a genetically independent, partially overlapping fashion with PCP signaling to regulate mediolateral cell polarity underlying axis extension in part by promoting notochord boundary formation. Gon4l limits expression of the cell–cell and cell–matrix adhesion molecules EpCAM and Integrinα3b, excesses of which perturb the notochord boundary via tension-dependent and -independent mechanisms, respectively. By promoting formation of this AP-aligned boundary and associated cell polarity, Gon4l coop- erates with PCP signaling to coordinate morphogenesis along the AP embryonic axis.

1 Department of Developmental Biology, Washington University School of Medicine, Saint Louis, MO 63110, USA. 2 Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA. 3Present address: Division of Pediatric Gastroenterology, Hepatology, and Nutrition, Cincinnati Children’s Hospital, Cincinnati, OH 45229, USA. Correspondence and requests for materials should be addressed to L.S-K. (email: [email protected])

NATURE COMMUNICATIONS | (2018) 9:1319 | DOI: 10.1038/s41467-018-03715-w | www.nature.com/naturecommunications 1 ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/s41467-018-03715-w

astrulation is a critical period of animal development PCP signaling to coordinate morphogenesis that extends the AP during which the three primordial germ layers—ecto- embryonic axis. G — fi derm, mesoderm, and endoderm are speci ed, patterned, and shaped into a rudimentary body plan. During Results vertebrate