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A New Species of Mouse Spider (Actinopodidae, Missulena) from the Goldfields Region of Western Australia

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A New Species of Mouse Spider (Actinopodidae, Missulena) from the Goldfields Region of Western Australia Evolutionary Systematics 1 2017, 39–46 | DOI 10.3897/evolsyst.1.14665 A new species of Mouse Spider (Actinopodidae, Missulena) from the Goldfields region of Western Australia Volker. W Framenau1,2,3,4, Danilo Harms4 1 Phoenix Environmental Sciences Pty Ltd, 1/511 Wanneroo Road, Balcatta, Western Australia 6021, Australia 2 School of Animal Biology, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6009, Australia 3 Department of Terrestrial Zoology, Western Australian Museum, Locked Bag 49, Welshpool DC, Western Australia 6986, Australia 4 Zoological Museum, Center of Natural History, University of Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany http://zoobank.org/6FB6F2EA-4F6F-44ED-A644-DB99C9411E36 Corresponding author: Volker W. Framenau ([email protected]) Abstract Received 23 June 2017 Accepted 18 August 2017 A new species of Mouse Spider (family Actinopodidae Simon, 1892), Missulena hare- Published 22 December 2017 woodi, is described from near the City of Kalgoorlie-Boulder in the Goldfields region of Western Australia. It differs from all other Missulena species by the unusual light grey Academic editor: colouration of the abdomen in combination with small body size and shiny carapace. A Martin Husemann phylogenetic analysis of a fragment (658 bp) of the COI barcoding gene places M. hare- woodi sp. n. in a clade with four Missulena species from the Pilbara region of Western Australia, more than 900 km away. Missulena harewoodi sp. n. is one of the many spe- Key Words cies in this genus that are currently only known from a single, or a very limited number of specimen, highlighting the paucity of fauna collections in many arid regions of Australia Taxonomy and the difficulties in sampling these cryptic spiders. systematics barcoding COI Mygalomorphae Introduction red possibly relates to the diurnal activity of mate-search- ing Missulena males, whereas the majority of males in The spider family Actinopodidae Simon, 1892 has a other mygalomorph families are strictly nocturnal (Harms Gondwanan distribution with species found in Australia and Framenau 2013). and South America (World Spider Catalog 2017). In Aus- The Australian fauna in this family currently comprises tralia, these spiders are commonly called Mouse Spiders 16 species in the single genus Missulena Walckenaer, and widely known because they can be quite large (up to 1805 (Miglio et al. 2014; World Spider Catalog 2017). 3–4 cm in body size) and superficially resemble the ven- However, species-level diversity in this genus is clearly omous Funnel-web Spiders (family Hexathelidae Simon, much higher, with dozens of undescribed species identi- 1892). Some species may have a potent venom although fied in recent barcoding analyses (e.g. Castalanelli et al. spiders appear to generally apply a dry bite in defense 2014). The fauna of Western Australia, where 14 of the 16 (e.g. Isbister 2004; Isbister and Gray 2004; Rash et al. described species occur (Miglio et al. 2014), is particularly 2000). The colour patterns in male Missulena belong to diverse. Recent barcoding projects focusing on the Pilbara the most spectacular amongst all mygalomorph spiders. region in the northwest of the state recovered 13 distinct Most striking are the sometimes bright red fangs and/or genetic clades, only five representing described species carapace in males, iridescent blue abdomens, or an ab- (Castalanelli et al. 2014). The two most recent taxonomic domen with light colour patterns (Framenau et al. 2014). studies (Harms and Framenau 2013, Miglio et al. 2014) on Vivid colouration with typical warning colours such as Missulena developed a protocol for the description of spe- Copyright Volker W. Framenau, Danilo Harms. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 40 Volker W. Framenau & Danilo Harms: A new species of Mouse Spider (Actinopodidae, Missulena)... Table 1. Missulena specimen sequenced for this study. WAM Identification GenBank Sex Location Latitude Longitude registration (WAM database) accession no. T142820 M. harewoodi sp. n. male 20 km E of Kalgoorlie -30.744722 121.566944 MF693350 T124777 M. ‘DNA02’ immature 5 km WNW of Mt Farquhar -22.282259 116.720715 MF693351 T128168 M. ‘DNA03’ immature 100 km W of Tom Price -22.538166 116.869921 MF693352 T128170 M. ‘DNA03’ immature 100 km W of Tom Price -22.708678 116.850098 MF693353 T128154 M. ‘DNA03’ immature 100 km W of Tom Price -22.6009787 117.028344 MF693354 cies after several decades of taxonomic neglect and added designation as provided by the Western Australian Muse- six new species to the Australian fauna. They also provid- um (WAM) or Castalanelli et al. (2014). ed an updated key to the males of all Australian species Molecular analyses. Sequencing of the mitochondri- (Harms and Framenau 2013; Miglio et al. 2014). al CO1 gene of the holotype followed the protocol de- Recent collections as part of environmental assess- scribed in a previous study (Harms and Framenau 2013). ment studies in the semi-arid Goldfields region of West- Phylogenetic analyses were undertaken on an alignment ern Australia recovered a small male of Missulena with comprising all 84 Missulena sequences from previous the feature of light grey abdomen both as live (VWF pers. studies and available on GenBank (Castalanelli et al. obs.) and preserved (Fig. 1A) specimen, another facet of 2014; Harms and Framenau 2013), four specimens that the striking colour variations present within this genus. were sampled in Western Australia since then and the ho- Light abdomen discolourations have previously only lotype of M. harewoodi sp. n. (Table 1). COI sequenc- been described from two larger species with very differ- es of a single specimen of Euagrus chisoseus Gertsch, ent distribution in Australia (M. pruinosa Levitt-Gregg, 1939 (Dipluridae Simon, 1889) and five unidentified 1966 from tropical northern Australia and M. bradleyi specimens of Conothele Thorell, 1878 (family Ctenizidae Rainbow, 1914 from the subtropical/temperate eastern Thorell, 1887) were extracted from GenBank and includ- parts of the country), but the current species is smaller ed in the analyses as outgroups. A barcoding threshold of and the pattern is more pronounced. 9.5% pairwise sequence divergence was used for species In, this paper we describe this unusual species of Mis- delineation at a molecular level (Castalanelli et al. 2014). sulena based on male morphology. A phylogenetic anal- Phylogenetic analyses were executed using MrBayes ysis of a fragment of the CO1 barcoding gene is used to Version 3.2.1 for Macintosh (Ronquist et al. 2012), with explore the systematic position of this species and consol- the GTR+I+G model of nucleotide substitution suggested idate its taxonomic concept developed based on morphol- by MrModeltest Version 3.7 (Posada and Crandall 1998) ogy. In documenting this species, we hope to raise further for an unpartitioned dataset. Four Markov chain Monte awareness for the Australian Mouse Spiders by document- Carlo (MCMC) chains were run for 40,000,000 genera- ing another morphological rarity in its diverse fauna. tions, sampling every 1,000 generations and discarding the first of 25% of sampled trees as ‘burnin’. FigTree ver- sion 1.4.3 (Rambaut 2016) was used to visualise and edit Methods the tree. Morphology. The holotype of M. harewoodi sp. no. was examined in 75% ethanol under Leica M205C and Systematics M80 stereomicroscopes. Digital images were taken us- ing a Leica DFC 295 digital camera attached to the Lei- Family Actinopodidae Simon, 1892 ca M205C stereomicroscope controlled by the Leica Missulena Walckenaer, 1805 Application Suite Version 3.8. The images were edited and formatted in Adobe Photoshop CC, release 2017. For Missulena Walckenaer, 1805: 8. Type species: Missulena measurement protocols and overall format of the descrip- occatoria Walckenaer, 1805, by monotypy. tion please see Griswold and Ledford (2001), Harms and Eriodon Latreille, 1806: 85. Type species: Eriodon occa- Framenau (2013) and Miglio et al. (2014). Abbreviations torius Latreille, 1806, by monotypy. for morphological characters are as follows: (EL) embo- lar lamella, (DET) distal embolar tooth, (BEI) basal em- bolar intumescence, (PME) posterior median eyes, (PLE) Missulena harewoodi sp. n. posterior lateral eyes, (ALE) anterior lateral eyes, (AME) http://zoobank.org/3FE6A340-C871-45B8-ACFC-E9677586C417 anterior median eyes, (MOQ) median ocular quadrangle, Figs 1A–G, 2A–F, 4 (OAL) ocular area length, (OAW) ocular area width. Leg spination: (d) dorsal, (pv) proventral, (rv) retroventral, Type material. AUSTRALIA: Western Australia: ho- (v) ventral. WAM refers to the Western Australian Muse- lotype male, 20 km East of Kalgoorlie, 30°44’41”S, um. The nomenclature of undescribed species follows the 121°34’01”E, 14–16 April 2015, Greg Harewood, dry evolsyst.pensoft.net Evolutionary Systematics 1 2017, 39–46 41 Figure 1. Missulena harewoodi sp. n., male holotype (WAM T142820) A, habitus, dorsal view; B, ventral view; C, eye region, dorsal view; D, carapace, lateral view; E, sternum, ventral view; F, abdomen, dorsal view; G, maxillae and labium, ventral view. Scale bars: A, B – 2.0; C – 0.1; D, E, F – 1.0; G – 0.5. Arrows point to pair of anterior setae (C) and labio-sternal sigillae (E) (see text). evolsyst.pensoft.net 42 Volker W. Framenau & Danilo Harms: A new species of Mouse Spider (Actinopodidae, Missulena)... pitfall trap, Goldfields Blackbutt low woodland over open Labium: ca. 0.82 long, 0.70 wide; conical, 11 pointed scrub on loam (WAM T142820). cuspules anteriorly (Figs 1G); labiosternal suture poorly de- Etymology. The specific epithet is a patronym in hon- veloped; a pair of sigilla near labiosternal suture (Fig. 1E). our of Greg Harewood, the collector of the type specimen. Sternum: 2.17 long, 2.00 wide; pear-shaped and re- Diagnosis.
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