Plant Community Restoration on Mima Mounds at Turnbull National Wildlife Refuge, WA Brandy K

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Plant Community Restoration on Mima Mounds at Turnbull National Wildlife Refuge, WA Brandy K Eastern Washington University EWU Digital Commons EWU Masters Thesis Collection Student Research and Creative Works 2012 Plant community restoration on mima mounds at Turnbull National Wildlife Refuge, WA Brandy K. Reynecke Eastern Washington University Follow this and additional works at: http://dc.ewu.edu/theses Part of the Biology Commons Recommended Citation Reynecke, Brandy K., "Plant community restoration on mima mounds at Turnbull National Wildlife Refuge, WA" (2012). EWU Masters Thesis Collection. 62. http://dc.ewu.edu/theses/62 This Thesis is brought to you for free and open access by the Student Research and Creative Works at EWU Digital Commons. It has been accepted for inclusion in EWU Masters Thesis Collection by an authorized administrator of EWU Digital Commons. For more information, please contact [email protected]. Plant Community Restoration on Mima Mounds at Turnbull National Wildlife Refuge, WA A Thesis Presented to Eastern Washington University Cheney, Washington In Partial Fulfillment of the Requirements for the Degree Master of Science By Brandy K. Reynecke Summer 2012 Abstract: Prevention and management of exotic plant species invasion can diminish their negative ecological and economic impacts and maintain ecological functions provided by native species. This is particularly important in rare landscapes that provide essential habitat. The most successful management plans integrate techniques to reduce invasive species abundance, increase native species abundance and reduce invasibility. Eastern Washington’s Channeled Scablands bioregion has highly fragmented remnant patches of grassland underlain by glacial alluvium and basalt bedrock that are dotted with Mima mounds (naturally occurring aggregations of fine particle soil). The mounds provide valuable habitat for native plants and animals and many have been heavily invaded by exotic plant species, particularly winter annual grasses such as Bromus tectorum and Ventenata dubia . This study was designed to characterize Mima mound plant communities in the Channeled Scablands and to test the effects of restoration techniques on those communities. I conducted a vegetation survey to characterize community composition and developed three experiments to: 1) test effectiveness of Imazapic, sucrose soil amendment and native seed addition ( Pseudoroegnia spicata and Festuca idahoensis ), for exotic plant reduction on two geologic substrates (basalt and alluvium), 2) test effectiveness of a biocontrol insect (Mecinus janthiniformis) to reduce Linaria dalmatica , and 3) test effectiveness of an experimental strain of rhizobacteria, Pseudomonas fluorescens D7, for invasive annual grass reduction. I surveyed plant species composition and cover in 235 1 m 2 vegetation plots in June- July 2009, measured environmental variables for 203 of the plots, applied treatments in summer and fall 2009, and resurveyed plots in June-July 2010. Species assembly was analyzed using NMS ordination, indicator species and cluster analyses. Preliminary abundance comparisons between substrates were conducted using Dunnett’s T3 pair-wise comparisons for unequal iv variance. Mean change in abundance over time was compared among treatments using general linear models (GLM). Community analysis revealed ten community types and a distinct difference in species composition between substrate types. The experimental application of control techniques did not yield the expected results; treatments containing herbicide reduced all species, neither biocontrol agent reduced the abundance of target species, native seed addition had no effect and sucrose addition reduced native species cover but did not affect exotic cover. In control plots, invasive species cover increased significantly over the course of one year, suggesting that management is needed to prevent further invasion. Insight gleaned from both the community analyses and experimental results will be used to direct future study and guide adaptive management practices. v Acknowledgements I would like to thank my advisor Dr. Rebecca L. Brown for support on this project and for all of the long hours she spent with me in the field, at school, and in her home to make this project a success. I owe a debt of gratitude to my committee members Dr. Suzanne Schwab and Dr. Robin O’Quinn for being very candid and for imparting their expertise in the fields of botany and ecology. I would also like to thank Dr. Ross Black for his enthusiasm and sharing his statistical prowess. The advice and guidance I received from my professors has enabled me to grow professionally and personally. I would also like to extend my gratitude to Mike Rule, TNWR biologist, for his advice and guidance on this project as well as for securing funding through a grant from the US Fish and Wildlife Service. I also received funding through a mini-grant from EWU. I want to thank Jessica Bryant for her extensive assistance in experimental design, surveying and treatment application. Also I want to thank my field crew, Holly O’Connor, Brittany Davidson, Cara Hulce, Jenni Grimes, Joe Miles, Trenton Reynecke, Aaron Clausen, Dash Hibbard and Mario Lawrence. Without your assistance in the field I could not have completed this project. I would like to thank Dr. Ann Kennedy at WSU for supplying the rhizobacteria and to Jeremy Hensen for applying it. Thanks to Larry Skillestad of the Spokane office USDA for supplying insects and for sharing his extensive knowledge. Thank you to Dr. Julie Beckstead for supplying me with materials for insect cages and insight on sucrose addition. vi Table of Contents Abstract ........................................................................................................................................iv Acknowledgements ......................................................................................................................vi List of Figures ..............................................................................................................................ix List of Tables ................................................................................................................................x Introduction ...................................................................................................................................1 Restoration approaches and techniques ............................................................................1 Prairie patches in the Channeled Scablands ......................................................................2 Mima mound prairies of eastern Washington ...................................................................3 Native plant community restoration approaches on Mima mounds .................................7 Objectives ….....................................................................................................................9 Methods ......................................................................................................................................10 Study site description ....................................................................................................10 Survey of Mima mound vegetation ...............................................................................14 Experimental design ......................................................................................................15 Experiment 1: Overall reduction of invasive species .........................................15 Experiment 2: Targeted control of Linaria dalmatica .......................................18 Experiment 3: P.f. D7 for targeted control of B. tectorum .................................20 Data analyses ..................................................................................................................22 Results ........................................................................................................................................23 vii Vegetation survey ...........................................................................................................23 Ordination analysis of 203 plot ......................................................................................24 Comparison of pre-treatment IAG abundance and richness across substrate type ........24 Experiment 1: Change in abundance and richness of invasive and native plants due to combinations of herbicide, native seed addition, sucrose soil amendment and geologic substrate ..........................................................................................................................30 Experiments 2 and 3: Effects of biocontrol agents on L. dalmatica and B. tectorum ....35 Discussion ..................................................................................................................................38 Community description ..................................................................................................38 Restoration technique experiments ................................................................................39 Literature cited ...........................................................................................................................40 Appendix I: Supplemental figures from NMS ordination and community analyses .................53 Appendix II: ANOVA / GLM tables for combined effects of herbicide, sucrose and native seed ............................................................................................................................................58 Appendix III: Change
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