Structural Approaches to Probing Metal Interaction with Proteins
Journal of Inorganic Biochemistry 115 (2012) 138–147 Contents lists available at SciVerse ScienceDirect Journal of Inorganic Biochemistry journal homepage: www.elsevier.com/locate/jinorgbio Structural approaches to probing metal interaction with proteins Lorien J. Parker a,1,2, David B. Ascher a,2, Chen Gao a,2, Luke A. Miles a,b,c,3, Hugh H. Harris d,3, Michael W. Parker a,c,⁎,3 a Biota Structural Biology Laboratory, St. Vincent's Institute of Medical Research, 9 Princes Street, Fitzroy, Victoria 3065, Australia b Department of Chemical and Biomolecular Engineering, The University of Melbourne, Victoria 3010, Australia c Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Road, Parkville, Victoria 3010, Australia d School of Chemistry and Physics, University of Adelaide, South Australia 5005, Australia article info abstract Article history: In this mini-review we focus on metal interactions with proteins with a particular emphasis on the evident Received 1 December 2011 synergism between different biophysical approaches toward understanding metallobiology. We highlight Received in revised form 2 February 2012 three recent examples from our own laboratory. Firstly, we describe metallodrug interactions with glutathi- Accepted 20 February 2012 one S-transferases, an enzyme family known to attack commonly used anti-cancer drugs. We then describe a Available online 3 March 2012 protein target for memory enhancing drugs called insulin-regulated aminopeptidase in which zinc plays a role in catalysis and regulation. Finally we describe our studies on a protein, amyloid precursor protein, Keywords: Amyloid precursor protein that appears to play a central role in Alzheimer's disease.
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