AR TICLE Basidioascus Undulatus: Genome, Origins, and Sexuality

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AR TICLE Basidioascus Undulatus: Genome, Origins, and Sexuality IMA FUNGUS · 6(1): 215–231 (2015) [!644"E\ 56!46F6!!7 Basidioascus undulatus: genome, origins, and sexuality ARTICLE 9 <V>!5< G 3 9 !5 7 2 /@ !5 !< + W @ Z % Q _6U G Q Q 2!>F>4G K . [ !"E7^ 5+ % US G Q G / / .WG "F6G /%Q Q 2!/6GFG 3U G S G Q G / / .WG "F6G /%Q Q 2!/ 6GFG 7@* @ / O @ Z % V/E45E#.!F6!Z@/ Abstract: Basidioascus undulatus Geminibasidiales Key words: V $ % [ Agaricomycotina WallemiomycetesV Geminibasidiales % [$ N Basidiomycota ? B. undulatus $ % Geminibasidiomycetes V $ _5U O % 57 F!5_ ; % B. undulatus Wallemiomycetes %$ N [ $ %% Z _4 . $ [ B. undulatus Wallemia AgaricomycotinaV % Geminibasidiales Wallemiomycetes Geminibasidiomycetes%W Agaricomycotina Wallemiomycetes Geminibasidiomycetes Article info:@ [!FU 56!4K/ [!_`56!4K; [!"`56!4 INTRODUCTION undulatus Basidioascus, B. magus/ . B. persicus Z U 566_ N > et al.56!7@ Basidioascus undulatus Basidioascus Basidioascus 9% G V > ; / U 566_ <et al.!"E6 B. undulatus, which he @ <>/? % Basidioascus Wallemiomycetes O V Basidiomycota SaccharomycetesAscomycota Basidioascus O . > et al. 56!_V [ % P undulatus % $ . O % P U 566_ V [ / B. undulatus . $ U 566_ O . U+ et al.56!_ Ainsworth & Bisby’s Dictionary of the Fung 2 et al.566E [ U B. undulatus in Saccharomycetes $ % Geotrichum [ > et $ $ al. 56!_ V Basidioascus, Galactomyces Dipodascus. GeminibasidiumG. donsium G. hirsutum < % G [ Geminibasidiales > et > et al. 56!_ B. al. 56!_ V Geminibasidiales © 2015 International Mycological Association You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution: [ Non-commercial: No derivative works: For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights. VOLUME 6 · NO. 1 215 Nguyen et al. Wallemiales % "_ ? De novo Wallemiomycetes > et al. 56!_ Wallemiales @;/%_6+ % et al.56!5 Wallemia with three + 9 > et al. 56!_ [W. sebi, W. muriae W. ichthyophaga et [ .. al.5664V Wallemiomycetes 5! _! 7! 4! F! #! E! "! W ARTICLE [ U et @@;/GS%56+ O et al. al. 566F 56!! [.$!.74.!6. 6. 6#.4. 6#. !6.O5666.V!F.! 5666 % 9% / Z/@V %5_] % et al.56!_V + 5 % Agaricomycotina %5!6 * I @ O 56!5/ ; et al.56!5 et al.56!_ + 5 @/U % +/U N [ % @/U %6!!"* et al.566" $ B. undulatus % %6E!] . % / et al.56!5V [ > et al. 56!_ W GS]U/ % 54 ; et al. 566# $ % % GS] W ? B. undulatus ] %% 9 et al. 56!! ?. [ Q % % U % 764 [\\ . % Geminibasidiales in Wallemiomycetes [ . . _4 [\\ \ V . B. undulatus U/2S5%5!69 I % 56!! N U/2S5 B. undulatus Wallemia . The ]U .S@%5_+ %I* O % % 56!!ab initio >G+N % [ @? ? . $ %556@ I+ 5664; Agaricomycotina % < et al. 566" $ % $ B. undulatus, G. donsium, W. sebi $ ? . ?G<@; % [ Geminibasidiales $ V ? . N ; @ 4GF ` et al. 56!7 [ MATERIALS AND METHODS ... . Z ; \@ .; Growth, DNA extraction and sequencing %555EV U* % V$. Basidioascus undulatus</QU57!"4F 555E N ; @ + 5]Q%5#! 5z $ S G et al.5664 \ 54G 5V % [ ? V 46*W +*/@V % .% !6S.!66 !6666& 4 V ? % 1#6z V O ? % % [\\ <>/ $ \\ >/R Q ; ].+ @ * UQ >/ % !5 * et al. 566# < R <>/ ? ? [ % >G+N ]/ > % [ * V + G `V*@66666666% `V*@6!666666K+ ; / .? !6! . >;`>/57#""5 `]NUG ] % et N 9 @?5466 V @?X_ al.56!7 > G G @ @ #" +*/@V [ Basidioascus undulatus Genome assembly and annotation V ? V ? % . % W G%6!6! [\\ \ +*/@V \ ?\ Z $ W/@V. %555EG et al.566" V % 66!_K [\\ \ $ \ ] % %% 4R in Saccharomyces cerevisiae Cryptococcus neoformans 216 IMA FUNGUS Basdioascus genome, origins, and sexuality 9 et al.56!! ? .% % ;9*N; @ X ARTICLE ¢!6S.64 B. undulatus +*/@V %74_]et al.56!6V % 555E Saccharomyces cerevisiae C. O % neoformans ;+ _ %!7 * et al. 56!_ * . [ et al. 566" G/V.]V * I ; 9 et al.56!! 5667 / % U % O{!66 $ ¢6! [ N ; @ % 555E [.$!66646 ! 5 Saccharomyces cerevisiae _ [.$!666 ! 5 _ ? .% ¢!6S.64 / Q $R Phylogenomics and molecular dating . O% ;+ _ %_5 ; ? Agaricus bisporus . % bisporus 9"# % 56 U et al 56!5 Alternaria % + % [ . brassicicola Q et al 56!5 Arthrobotrys oligospora .V . $ . /VGG 57"5# et al 56!! Aureobasidium pullulans $ . ! Rhizophagus irregularisGlomeromycota % pullulans SW.!46 ] et al 56!7 Auricularia [ < % subglabra % 56 W et al 56!5 Botrytis cinerea% 9 et al. !""# !6 @ % 2 56!5 Coccidioides immitis @ @ et al. 5667V I + 566# + IV @ et al 566" Coprinopsis cinerea @ et al 56!6 ; I 56!_K $ 56!6 Cryptococcus neoformans % neoformans `SG5! % [ ? G * et al 5664 Dacryopinax <`U #_! @@;! % % [.$!66646 !6 W et al 56!5 Fomitiporia mediterranea % !6 ! ;+ _ W et al 56!5 Fomitopsis pinicola W;.4E45# @@! %_6 W et al 56!5 Malassezia globosa et al Confocal laser scanning microscopy 566# Mixia osmundae N/U !7_57 % !6 V et al V % 56!7 Monacrosporium haptotylumG+@56646U Basidioascus undulatus</QU57!"4F et al56!_Neurospora crassaQ#7/%56] et GU// U * al566_Penicillium chrysogenum 47.!544% UN ! <>/ [</;N.W . + et al566EPuccinia striiformistritici;@V. 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