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ARHGAP25 (A-12): Sc-137297 SAN TA C RUZ BI OTEC HNOL OG Y, INC . ARHGAP25 (A-12): sc-137297 BACKGROUND SOURCE GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP ARHGAP25 (A-12) is an affinity purified goat polyclonal antibody raised hydrolysis of Ras-related proteins, resulting in downregulation of their active against a peptide mapping within an internal region of ARHGAP25 of human form. ARHGAP25 (Rho GTPase activating protein 25), also known as Rho-type origin. GTPase-activating protein 25, is a 645 amino acid protein that contains one Pleckstrin homology (PH) domain and one Rho-GAP domain. Encoded by a PRODUCT gene that maps to human chromosome 2p14, ARHGAP25 exists as four alter - Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide natively spliced isoforms and shares significant homology with ARHGAP22 and 0.1% gelatin. and ARHGAP24 by exhibiting a common domain structure (PH-RhoGAP-CC); however, tissue expression of ARHGAP25 is myeloid-specific. ARHGAP25 is Blocking peptide available for competition studies, sc-137297 P, (100 µg a candidate epigenetic biomarker for non-invasive prenatal diagnosis of Down peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). syndrome, as well as a candidate gene in a chromosome 2p susceptibility locus linked to salt-sensitive hypertension and drug response. APPLICATIONS ARHGAP25 (A-12) is recommended for detection of ARHGAP25 isoforms 1 REFERENCES and 2 of mouse, rat and human origin by Western Blotting (starting dilution 1. Katoh, M., et al. 2004. Identification and characterization of ARHGAP24 1:100, dilution range 1:50-1:500), immunofluorescence (starting dilution 1:25, and ARHGAP25 genes in silico. Int. J. Mol. Med. 14: 333-338. dilution range 1:25-1:250) and solid phase ELISA (starting dilution 1:30, dilu - tion range 1:30-1:3000); non cross-reactive with other ARHGAP family 2. Hjelmervik, T.O., et al. 2005. Gene expression profiling of minor salivary mem bers. glands clearly distinguishes primary Sjögren’s syndrome patients from healthy control subjects. Arthritis Rheum. 52: 1534-1544. ARHGAP25 (A-12) is also recommended for detection of ARHGAP25 isoforms 1 and 2 in additional species, including equine, canine, bovine 3. Kandpal, R.P. 2006. Rho GTPase activating proteins in cancer phenotypes. and porcine. Curr. Protein Pept. Sci. 7: 355-365. Suitable for use as control antibody for ARHGAP25 siRNA (h): sc-94483, 4. Padmanabhan, S., et al. 2006. Chromosome 2p shows significant linkage ARHGAP25 siRNA (m): sc-141212, ARHGAP25 shRNA Plasmid (h): to antihypertensive response in the British genetics of hypertension study. sc-94483-SH, ARHGAP25 shRNA Plasmid (m): sc-141212-SH, ARHGAP25 Hypertension 47: 603-608. shRNA (h) Lentiviral Particles: sc-94483-V and ARHGAP25 shRNA (m) 5. Ohta, Y., et al. 2006. FilGAP, a Rho- and ROCK-regulated GAP for Rac binds Lentiviral Particles: sc-141212-V. filamin A to control actin remodelling. Nat. Cell Biol. 8: 803-814. Molecular Weight of ARHGAP25: 73 kDa. 6. Old, R.W., et al. 2007. Candidate epigenetic biomarkers for non-invasive prenatal diagnosis of Down syndrome. Reprod. Biomed. Online 15: 227- 235. RECOMMENDED SECONDARY REAGENTS 7. Newkirk, K.M., et al. 2008. Microarray analysis demonstrates a role for To ensure optimal results, the following support (secondary) reagents are Slug in epidermal homeostasis. J. Invest. Dermatol. 128: 361-369. recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey 8. Thakur, A., et al. 2008. Gene expression profiles in primary pancreatic anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ tumors and metastatic lesions of Ela-c-myc transgenic mice. Mol. Cancer Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 7: 11. sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- 9. Yi, P., et al. 2010. Key genes and proteins involved in CTCM-reducing rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- microvascular endothelial cell permeability induced by SLT-IIv using gene 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) chips and DIGE. Cell. Immunol. 265: 9-14. with UltraCruz™ Mounting Medium: sc-24941. CHROMOSOMAL LOCATION PROTOCOLS Genetic locus: ARHGAP25 (human) mapping to 2p13.3; Arhgap25 (mouse) See our web site at www.scbt.com or our catalog for detailed protocols and mapping to 6 D1. support products. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. RESEARCH USE For research use only, not for use in diagnostic procedures. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.
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