DOCSLIB.ORG

NGF-TRKA ENDOSOME DYNAMICS, SIGNALING and FUNCTION in SYMPATHETIC NEURON DENDRITES by Kathryn M. Lehigh a Dissertation Submitted

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
NGF-TRKA ENDOSOME DYNAMICS, SIGNALING and FUNCTION in SYMPATHETIC NEURON DENDRITES by Kathryn M. Lehigh a Dissertation Submitted NGF-TRKA ENDOSOME DYNAMICS, SIGNALING AND FUNCTION IN SYMPATHETIC NEURON DENDRITES by Kathryn M. Lehigh A dissertation submitted to Johns Hopkins University in conformity with the requirements of the degree of Doctor of Philosophy Baltimore, Maryland May, 2016 Abstract Nerve growth factor (NGF) is the prototypical neurotrophin, playing key roles in cell growth, survival, and target innervation as well as dendritic growth and the formation and maintenance of synaptic connections. Sympathetic neurons are dependent on target- derived NGF for survival and as such have become an exemplary model for studying NGF function. NGF signals via retrogradely transported NGF-TrkA endosomes. Recently we have discovered the retrograde transport of TrkA endosomes into the dendritic compartment of sympathetic neurons where they may contribute to the formation and organization of synapses. In this study, we developed a real time imaging paradigm to examine the mobility and transport of TrkA in dendrites, comparing findings to TrkA endosome movement and transport in axons and cell bodies. Using this method we observe that after application of NGF to the distal axon compartment of cultured neurons, TrkA endosomes move in a saltatory manner retrogradely through the axon, slow down or halt in the soma, and move in a bidirectional manner in dendrites. Although TrkA endosomes in dendrites move at a comparable rate to those in axons, their unique dynamics (i.e. more direction changes) result in a smaller net displacement than endosomes in axons. Further, immunocytochemistry using antibodies specific for a TrkA phosphorylated residue (Y785) that supports downstream signaling cascades of the NGF- TrkA complex demonstrates that retrogradely trafficked TrkA endosomes within dendrites are signaling competent and that P-TrkA positive endosomes juxtapose postsynaptic density complexes, in vitro and in vivo. These findings suggest that target- derived NGF-TrkA endosomes signal within dendrites to form and maintain synapses. Functional experiments that combine chemical genetics with drug loaded PLGA ii microspheres allow for spatially specific inhibition of TrkA kinase activity and have revealed that TrkA activity is necessary in the somatodendritic compartment for both synapse formation (in vitro) and maintenance (in vivo). We have achieved the ability to inhibit TrkA kinase activity in dendrites of cultured neurons, observing that retrogradely transported TrkA endosomes signal within dendrites to maintain PSD clusters. This work reveals a novel mode of NGF-dependent synapse formation and maintenance, and the mechanism by which target fields control circuit assembly. Thesis Advisor and Reader #1 David Ginty, Ph.D. Committee Member and Reader #2 Rick Huganir, Ph.D. Committee Member Mollie Meffert, Ph.D. Committee Member Larry Schramm, M.D. Ph.D. iii Acknowledgements The greatest lesson I have learned through the completion of my dissertation is that both science and life are better as collaborative efforts. First, I wish to thank my advisor and mentor Dr. David Ginty because his enthusiasm for good, interesting science always invigorated me, and consequently, my project. I think that David is a scientist for all the right reasons and that has created an incredible lab environment in which to learn how to think critically and innovate. I also want to thank the members of my thesis committee who were always willing to discuss the thesis and contributed valuable insights, ideas, and suggestions: Dr. Richard Huganir, Dr. Larry Schramm, and Dr. Mollie Meffert. I am grateful to Rick, for his strong support and encouragement and for volunteering to be a careful reader of this thesis. Larry for generously providing me with hard copies of innumerable papers documenting the history of the sympathetic nervous system and nerve growth factor field--from him I learned to appreciate that our work stands on the shoulders of those who came before us. I am eternally grateful to Mollie for welcoming me into her lab to use her spinning disk microscope; it was there that I first saw the trafficking of endosomes in real time and experienced a memorable moment of joyful discovery. I am also thankful to Beth and Rita in the Hopkins Neuroscience department for getting stuff done, being supportive, and always showing compassion during the difficult moments of graduate school. The long hours and roller coaster emotions that accompany experimental science turn co- workers into friends. Moving from one university to another turns co-workers into family. I’m grateful to all members of my Hopkins and Harvard Ginty lab family for support, advice, reagents, and scientific discussion. A lab of our size doesn’t run on its own; Dori, Sarah, Jessie, and Steve have been invaluable to making the Ginty lab an excellent home for six years. Particular thanks to past members whom I consider mentors—Coryse, Tony, Kevin, and Tracy, and to current members whom I turn to daily—Lauren, Vicky, and Emily. There’s a forever special bond between myself, Emily, and Krissy, who were not only my baymates but also my roommates through our not always smooth transition. iv All of my work has been made possible by incredible core staff members at both Hopkins and Harvard. Thank you Michele Pucak, Huy Vo, Daniel Tom, Lai Ding, Michelle Ocana, and Hunter Eliot for being so conscientious with your work and for taking the time to make my life easier and science better. I’m so grateful for my friendships forged through grad school trials and tribulations; business meetings with Clint and coffee meetings with Alex, Claudia and Erin are amazing memories. I feel so lucky that I have the most wonderful volleyball, book club, roommate, home and college friendships to rejuvenate me outside of the lab. Of course, the best support system of all has been my family. I could never thank my Mom and Dad enough for everything they have done for me. Nate has made me beautiful neuron drawings and has listened to every struggle whether in person or in email. Fortunately, he also married Justine who always kept me well fed. Nick, Becky, and Danny are amazing siblings, I’m grateful that they think I’m smart enough to do this and (sometimes) find it cool. Finally, I’m so happy I have Ron, who may have come along last but his support of my career has been the most tangible. Simply put, thanks for moving to Boston and for being my life partner. Last, I have to express gratitude for the opportunity to pursue my scientific interests, and for the incredible mentorship of Dr. Maria Donoghue who jumpstarted my scientific career. As David once said to me, “as scientists we get to discover things no one else knows, and it really doesn’t get any better than that”. v Table of Contents Title Page…………………………………………………………………………………..i Abstract……………………………………………………………………………………ii Acknowledgements……………………………………………………………………….iv Table of Contents…………………………………………………………………………vi List of Figures……………………………………………………………………………vii Chapter 1. Introduction……………………………………………………………………1 Chapter 2. Trka endosome dynamics, signaling and function in sympathetic neuron dendrites…………………………………………………………………………………19 Chapter 3. Discussion and Future Directions……………………………………………81 Materials and Methods………………………………………………………………….100 References………………………………………………………………………………112 Curriculum Vitae……………………………………………………………………….127 vi List of Figures Figure 2.1. Live cell assay to monitor endogenous receptor TrkA trafficking. Figure 2.2. Dynamics of axonal TrkA endosomes. Figure 2.3. The dynamics of TrkA endosomes in cell bodies. Figure 2.4. Dynamics of TrkA endosomes in dendrites. Figure 2.5. Comparison of TrkA endosome dynamics within different cellular compartments. Figure 2.6. Flag-TrkA endosomes from the target are transcytosed to CB/dendrite plasma membrane. Figure 2.7. Specificity of phosphorylated TrkA antibodies in vitro. Figure 2.8. Target-derived TrkA endosomes in dendrites are signaling competent. Figure 2.9. Target-derived Flag-TrkA endosomes are found in close proximity to PSDs. Figure 2.10. Target-derived signaling endosomes are located in close proximity to PSDs. Figure 2.11. Assay for visualizing target-derived vesicles in vivo. Figure 2.12 Target-derived endosomes containing TrkA are transported into dendrites in vivo. Figure 2.13 Specificity of phosphorylated TrkA antibody in vivo. Figure 2.14 TrkA signaling endosomes are found in close proximity to synapses in vivo. Figure 2.15 Ipsilateral injection of PLGA-1NM-PP1 microspheres results in local inhibition of TrkA kinase activity. Figure 2.16 PLGA-1NM-PP1 microspheres, not control PLGA microspheres, specifically F592A inhibit TrkA kinase activity in TrkA mice. Figure 2.17 Local inhibition of TrkA kinase activity in the SCG decreases number of synaptic puncta. Figure2.18 NGF-TrkA signaling is required for synapse formation in cell body/dendrite compartment. Figure 2.19 PLGA microspheres loaded with 1NM-PP1 inhibit TrkA kinase signaling and PSDs. Figure 2.20 In vitro assay for local inhibition of TrkA kinase activity within dendrites. Figure 2.21 Local TrkA kinase activity maintains synapses. vii Figure 3.1 Summary: Target-derived NGF-TrkA endosomes signal for synaptic maintenance. viii Chapter 1 Introduction 1.1. The sympathetic nervous system is an important and accessible system for the study of system and circuit formation At the heart of the study of neuroscience is the question of how individual neurons communicate with one another, coordinating the complex functions that are attributed to the central and peripheral nervous
Load more

Recommended publications
  • GSK3 Signalling Regulates Mammalian Axon Regeneration by Inducing the Expression of Smad1
    ARTICLE Received 28 Mar 2013 | Accepted 27 Sep 2013 | Published 28 Oct 2013 DOI: 10.1038/ncomms3690 PI3K–GSK3 signalling regulates mammalian axon regeneration by inducing the expression of Smad1 Saijilafu1,*, Eun-Mi Hur1,2,*, Chang-Mei Liu1, Zhongxian Jiao1, Wen-Lin Xu1 & Feng-Quan Zhou1,3 In contrast to neurons in the central nervous system, mature neurons in the mammalian peripheral nervous system (PNS) can regenerate axons after injury, in part, by enhancing intrinsic growth competence. However, the signalling pathways that enhance the growth potential and induce spontaneous axon regeneration remain poorly understood. Here we reveal that phosphatidylinositol 3-kinase (PI3K) signalling is activated in response to peripheral axotomy and that PI3K pathway is required for sensory axon regeneration. Moreover, we show that glycogen synthase kinase 3 (GSK3), rather than mammalian target of rapamycin, mediates PI3K-dependent augmentation of the growth potential in the PNS. Furthermore, we show that PI3K–GSK3 signal is conveyed by the induction of a transcription factor Smad1 and that acute depletion of Smad1 in adult mice prevents axon regeneration in vivo. Together, these results suggest PI3K–GSK3–Smad1 signalling as a central module for promoting sensory axon regeneration in the mammalian nervous system. 1 Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA. 2 Center for Neuroscience, Brain Science Institute, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea. 3 The Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA. * These authors contributed equally to this work.
    [Show full text]
  • At Storrs Th 5:00 Pm5:00 Universityconnecticutof Annual – Laurelhall,Floor First – 8:30 Pm 8:30 Th , 2016 , ITINERARY 5 Pm – 6 Pm …… Keynote Lecture
    An event organized by the HERE PLACE PLACE UCONN Interdisciplinary STAMP Neuroscience Program Steering Committee with the support of the 20th Annual UCONN OVPR Scholarship Facilitation Fund Neuroscience and the contribution of the at Storrs departments of Biomedical Engineering Electrical & Computer Eng. Monday, October 24th, 2016 Pharmaceutical Sciences Physiology & Neurobiology Psychological Sciences and the CT Institute for the Brain For more info visit 5:00 pm – 8:30 pm and Cognitive Sciences http://neuroscience.uconn.edu/ University of Connecticut Storrs Campus – Laurel Hall, First Floor ITINERARY 5 pm – 6 pm …… Keynote Lecture David Ginty, Ph.D. Professor, Harvard Medical School, Boston MA 6 pm – 8:30 pm ......Poster Session & Reception During the poster session, Ph.D. students, postdoctoral fellows, and researchers from across campus will present their work in Keynote Lecture Keynote Speaker poster format. Everybody is welcome to interact informally over food and drinks. A Molecular-genetic David Ginty, Ph.D. Professor, Harvard Medical 7 pm – 8 pm ……………. Data Blitz Approach to Decoding School Investigator, Howard Hughes The Data Blitz is a fun way for trainees to the Sense of Touch Medical Institute present their research in a concise manner http://gintylab.hms.harvard.edu/ to a diverse audience by encapsulating Abstract: The somatosensory system endows us Bio: Dr. Ginty received a degree in biology from Mount their work in a 3 minute-long presentation with a remarkable capacity for object recognition, Saint Mary’s College (1984) and a Ph.D. in physiology and limited to only 3 PowerPoint slides. The texture discrimination, sensory-motor feedback, from East Carolina University School of Medicine bell will be rung at the end of the 3 and social exchange.
    [Show full text]
  • Alumni Director Cover Page.Pub
    Harvard University Program in Neuroscience History of Enrollment in The Program in Neuroscience July 2018 Updated each July Nicholas Spitzer, M.D./Ph.D. B.A., Harvard College Entered 1966 * Defended May 14, 1969 Advisor: David Poer A Physiological and Histological Invesgaon of the Intercellular Transfer of Small Molecules _____________ Professor of Neurobiology University of California at San Diego Eric Frank, Ph.D. B.A., Reed College Entered 1967 * Defended January 17, 1972 Advisor: Edwin J. Furshpan The Control of Facilitaon at the Neuromuscular Juncon of the Lobster _______________ Professor Emeritus of Physiology Tus University School of Medicine Albert Hudspeth, M.D./Ph.D. B.A., Harvard College Entered 1967 * Defended April 30, 1973 Advisor: David Poer Intercellular Juncons in Epithelia _______________ Professor of Neuroscience The Rockefeller University David Van Essen, Ph.D. B.S., California Instute of Technology Entered 1967 * Defended October 22, 1971 Advisor: John Nicholls Effects of an Electronic Pump on Signaling by Leech Sensory Neurons ______________ Professor of Anatomy and Neurobiology Washington University David Van Essen, Eric Frank, and Albert Hudspeth At the 50th Anniversary celebraon for the creaon of the Harvard Department of Neurobiology October 7, 2016 Richard Mains, Ph.D. Sc.B., M.S., Brown University Entered 1968 * Defended April 24, 1973 Advisor: David Poer Tissue Culture of Dissociated Primary Rat Sympathec Neurons: Studies of Growth, Neurotransmier Metabolism, and Maturaon _______________ Professor of Neuroscience University of Conneccut Health Center Peter MacLeish, Ph.D. B.E.Sc., University of Western Ontario Entered 1969 * Defended December 29, 1976 Advisor: David Poer Synapse Formaon in Cultures of Dissociated Rat Sympathec Neurons Grown on Dissociated Rat Heart Cells _______________ Professor and Director of the Neuroscience Instute Morehouse School of Medicine Peter Sargent, Ph.D.
    [Show full text]
  • Iwyeth Been Identified in Many Diverse Animal Species and Also in Mammalian Viruses
    Conference Lecture Series Courses About the Workshop This workshop will be a unique chance to regroup and share results from scientists interested in semaphorins and their receptors . Submission of Applications • 10fellowships of 600 euros each will be provided by the "Ecole des Neurosciences de Paris" to students presenting posters at the meeting to cover the registration cas t. Students will be selected among the registered participants by the organizers. The Semaphorins are one of the t?l\111 Boehri~ger largest family of axon guidance "'llIhv Ingelhelm molecules, with more than 25 distinct genes characterized in vertebrates and invertebrates. Semaphorins have IWyeth been identified in many diverse animal species and also in mammalian viruses. Semaphorins participate in a variety of developmental and pathological processes. In vitro most semaphorins have potent repulsive effects on specific classes of embryonic axons, although some exert attractive effects. In addition to their ro Ie in axon guidance, many results suggest that semaphorins are involved in other developmental or pathological processes, such as apopt osis, tumorigenesis, angiogenesis, neurodegenerative diseases Conference Lecture Series Courses Speakers (confirmed) Britta Eickholt INSERMU841 MRC Centre for Developmental Neurobiology Faculte de MMecine Kings College London Submission of 8 rue du General Sarrail New Hunts House, Guys Campus Applications 94010 Creteil Strand, London WC2R 2LS France U.K [email protected] David Ginty Mary C. Halloran The Solomon H. Snyder Department of Neuroscience University of Wisconsin-Madison Zoology Howard Hughes Medical Institute 307 Zoo Research The Johns Hopkins University School of Medicine 1300 University Ave. Room 10 15, PCfB Madison, WI 53706-1532 t?l\111 Boehri~geJ 725 N.
    [Show full text]
  • Historical Perspective Neuroscience at Johns Hopkins
    CORE Metadata, citation and similar papers at core.ac.uk Provided by Elsevier - PublisherNeuron, Connector Vol. 48, 201–211, October 20, 2005, Copyright ª2005 by Elsevier Inc. DOI 10.1016/j.neuron.2005.10.005 Neuroscience at Historical Perspective Johns Hopkins Solomon H. Snyder* ing these talents and himself making major contribu- Department of Neuroscience tions to pathology. William Osler defined the field of in- Johns Hopkins University School of Medicine ternal medicine, and William Halstead inaugurated 725 North Wolfe Street modern surgery. There was no Neurology Department Baltimore, Maryland 21205 nor even a neurology division of Medicine. Neurosurgery remained a subdivision of the surgery department for al- most 70 years till Donlin Long was appointed the direc- In 1979, Joshua Lederburg, recently appointed presi- tor of a new Neurosurgery Department. dent of Rockefeller University, was in recruiting mode. Based on his long-term interest in the brain and psychi- Neurosurgery and Systems Neuroscience atry, Josh approached me with an attractive offer for In 1906, Harvey Cushing was appointed the first head of myself and my colleagues Joe Coyle and Mike Kuhar. neurosurgery at Hopkins (Figure 1). He revolutionized pi- I visited our Dean, Richard Ross, to say goodbye, as tuitary surgery and, by carefully monitoring symptoms I knew that Hopkins could never provide us Rockefeller- following removal of pituitary tumors, he was able to elu- like resources. While Ross couldn’t match the Rockefel- cidate the role of excess or deficient secretion of the an- ler offer for a professor, he had an alternative proposal. terior pituitary and to confirm his clinical observations Years earlier, an advisory committee had recommended with studies in animals (Cushing, 1909).
    [Show full text]
  • Poly(ADP-Ribosyl)Ation Basally Activated by DNA Strand Breaks Reflects Glutamate–Nitric Oxide Neurotransmission
    Poly(ADP-ribosyl)ation basally activated by DNA strand breaks reflects glutamate–nitric oxide neurotransmission Andrew A. Pieper*, Seth Blackshaw*, Emily E. Clements†, Daniel J. Brat‡, David K. Krug*, Alison J. White†, Patricia Pinto-Garcia†, Antonella Favit†, Jill R. Conover†, Solomon H. Snyder*§, and Ajay Verma† *Departments of Neuroscience, Pharmacology and Molecular Sciences, and Psychiatry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205; †Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814; and ‡Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 1364 Clifton Road Northeast, Atlanta, GA 30322 Contributed by Solomon H. Snyder, December 8, 1999 Poly(ADP-ribose) polymerase (PARP) transfers ADP ribose groups specific neuronal regions of the brain and in peripheral tissues. from NAD؉ to nuclear proteins after activation by DNA strand Basal in vivo neuronal PARP activity and DNA damage are breaks. PARP overactivation by massive DNA damage causes cell diminished, whereas basal NADϩ levels are elevated after ,death via NAD؉ and ATP depletion. Heretofore, PARP has been treatment with NMDA-R antagonists, free radical scavengers thought to be inactive under basal physiologic conditions. We now and nNOS inhibitors, as well as in nNOSϪ͞Ϫ mice. report high basal levels of PARP activity and DNA strand breaks in discrete neuronal populations of the brain, in ventricular ependy- Materials and Methods mal and subependymal cells and in peripheral tissues. In some We employed, at 2 weeks, primary rat cerebral cortical neurons peripheral tissues, such as skeletal muscle, spleen, heart, and (16), cerebellar granule cells (23), and cerebral cortical astro- kidney, PARP activity is reduced only partially in mice with PARP-1 cytes (24).
    [Show full text]
  • Biphasic Functions for the GDNF-Ret Signaling Pathway in Chemosensory
    Biphasic functions for the GDNF-Ret signaling pathway PNAS PLUS in chemosensory neuron development and diversification Christopher R. Donnellya, Amol A. Shaha, Charlotte M. Mistrettaa, Robert M. Bradleya, and Brian A. Pierchalaa,1 aDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109 Edited by Solomon H. Snyder, Johns Hopkins University School of Medicine, Baltimore, MD, and approved November 21, 2017 (received for review May 27, 2017) The development of the taste system relies on the coordinated on the soft palate as well as somatosensory neurons projecting to regulation of cues that direct the simultaneous development of the external ear (8). This major ganglion for orofacial sensation both peripheral taste organs and innervating sensory ganglia, but is complex and multimodal, with soma for taste, touch, and lin- the underlying mechanisms remain poorly understood. In this study, gual temperature reception (5–7). However, our knowledge of we describe a novel, biphasic function for glial cell line-derived the molecular mechanisms dictating GG chemosensory neuron neurotrophic factor (GDNF) in the development and subsequent development and postnatal heterogeneity remain poorly under- diversification of chemosensory neurons within the geniculate stood, especially compared with primary sensory afferent neurons in ganglion (GG). GDNF, acting through the receptor tyrosine kinase the DRG and TG. Ret, regulates the expression of the chemosensory fate determinant Previous studies have focused on the role of the neurotrophins Ret−/− Retfx/fx Phox2b early in GG development. mice, but not ; in chemosensory GG development and maintenance, with NT-4 Phox2b -Cre mice, display a profound loss of Phox2b expression with and BDNF emerging as the principal regulators of GG axon guid- subsequent chemosensory innervation deficits, indicating that Ret is ance (9), GG neuron survival (10), and CT nerve regeneration (11).
    [Show full text]
  • Basic Research Considerations for NINDS Analysis And
    Basic Research Considerations for NINDS Analysis and Recommendations Advisory Panel for Basic Research Gary L. Westbrook, MD, Vollum Institute, (Panel Chair) Dora E. Angelaki, PhD, Washington University School of Medicine Bruce Bean, PhD, Harvard Medical School David Bredt, MD, PhD, Eli Lilly and Company Dan Feldman, PhD, University of California, Berkeley David Ginty, PhD, Johns Hopkins University Sabine Kastner, MD, PhD, Princeton University Pat Levitt, PhD, University of Vanderbilt, Kennedy Center Earl Miller, PhD, Massachusetts Institute of Technology Robert H. Miller, PhD, Case Western Reserve University Joshua Sanes, PhD, Harvard University Leslie B. Vosshall, PhD, The Rockefeller University February, 2009 1 Table of Contents (Ctrl+Click to go to page) EXECUTIVE SUMMARY ................................................................................................ 4 PANEL CHARGE AND REPORT PROCESS ................................................................ 8 A. Charge 8 B. Process 8 FINDINGS AND RECOMMENDATIONS: ....................................................................... 9 I. NINDS SUPPORT FOR BASIC RESEARCH ............................................................ 9 A. Definition of Basic Research 9 B. A Unique Role for NINDS within the Biomedical Research Enterprise 9 C. Outreach and Education about the Importance of Basic Neuroscience Research 11 II. BALANCE OF THE NINDS BASIC RESEARCH PORTFOLIO .............................. 12 A. Scientific Distribution of Neuroscience Research at NINDS and NIH 12 B. Determination
    [Show full text]
  • Harvard University Program in Neuroscience
    Harvard University Program in Neuroscience History of Enrollment in The Program in Neuroscience July 2016 Updated each July Nicholas Spitzer, M.D./Ph.D. B.A., Harvard College Entered 1966 * Defended May 14, 1969 Advisor: David Poer A Physiological and Histological Invesgaon of the Intercellular Transfer of Small Molecules _____________ Professor of Neurobiology University of California at San Diego Eric Frank, Ph.D. B.A., Reed College Entered 1967 * Defended January 17, 1972 Advisor: Edwin J. Furshpan The Control of Facilitaon at the Neuromuscular Juncon of the Lobster _______________ Professor Emeritus of Physiology Tus University School of Medicine Albert Hudspeth, M.D./Ph.D. B.A., Harvard College Entered 1967 * Defended April 30, 1973 Advisor: David Poer Intercellular Juncons in Epithelia _______________ Professor of Neuroscience The Rockefeller University David Van Essen, Ph.D. B.S., California Instute of Technology Entered 1967 * Defended October 22, 1971 Advisor: John Nicholls Effects of an Electronic Pump on Signaling by Leech Sensory Neurons ______________ Professor of Anatomy and Neurobiology Washington University Richard Mains, Ph.D. Sc.B., M.S., Brown University Entered 1968 * Defended April 24, 1973 Advisor: David Poer Tissue Culture of Dissociated Primary Rat Sympathec Neurons: Studies of Growth, Neurotransmier Metabolism, and Maturaon _______________ Professor of Neuroscience University of Conneccut Health Center Peter MacLeish, Ph.D. B.E.Sc., University of Western Ontario Entered 1969 * Defended December 29, 1976 Advisor: David Poer Synapse Formaon in Cultures of Dissociated Rat Sympathec Neurons Grown on Dissociated Rat Heart Cells _______________ Professor and Director of the Neuroscience Instute Morehouse School of Medicine Peter Sargent, Ph.D.
    [Show full text]
  • MEETING REPORT Workshop on “SEMAPHORIN FUNCTION AND
    MEETING REPORT Workshop on “SEMAPHORIN FUNCTION AND MECHANISMS OF ACTION” Location: FRANCE. The meeting was held at the "Abbaye des Vaulx de Cernay" near Paris. Dates: May 8-11. 2008. APPLICANT: ANALIA RICHERI. PhD student. IIBCE. MONTEVIDEO, URUGUAY. Organizers of the workshop: Dr Alain Chédotal Dr Valérie Castellani Dr Alex Kolodkin CNRS UMR 7102 CNRS UMR 5534 The Solomon H. Snyder Departm Université Paris 6, Université Claude Bernard of Neuroscience Batiment B, Case 12, Lyon1, Bâtiment Mendel 741, Howard Hughes Medical Institute 9 Quai Saint Bernard, 2ème étage, 43 Bd du 11 Novem The Johns Hopkins School of 75005 Paris 1918 Medicine France 69622 Villeurbanne cedex 725 N. Wolfe St./1001 PCTB tel: (33) 144 273447/144 272130 France Baltimore, MD 21205 mail: [email protected] tel: (33) 472 432691 USA email: [email protected] Phone: (001) 410 6149499 email: [email protected] The expected benefits stated in my application were overwhelmed. My participation in the workshop gave me the opportunity to discuss with scientists working on different experimental systems involving semaphorins. I appreciated very much having the chance to discuss my own results and interact with Dr. Klagsbrun, Dr. A. Bagri, (angiogenesis and cancer), Christine Holt, Oded Behar (Neurvous System). Indeed, I was able to establish academic contact with Dr. A. Bagri. This collaboration allowed me to receive an antibody directed against one of the semaphorins receptors, which will be essential for my future work. That is why I am strongly grateful for CAEN’s financial support that allowed me to attend this workshop as well for being awarded for my poster presentation by the European Molecular Biology Organization (EMBO).
    [Show full text]
  • Agenda Final
    Janelia Farm Conference: Constructing Neural Circuits, April 29-May 2, 2012 Sunday, April 29th 3:00 pm Check-in 6:00 pm Reception (Lobby) 7:00 pm Dinner 8:00 pm Welcome / Opening Remarks (Organizers) 8:05 pm Keynote Talk: Liqun Luo, HHMI/Stanford University Assembly of the fly olfactory circuit 9:05 pm Refreshments available at Bob’s Pub NOTE: All meals are in the Dining Room All talks are in the Seminar Room Posters are in the Lobby updated 03/26/12 1 Janelia Farm Conference: Constructing Neural Circuits, April 29-May 2, 2012 Monday, April 30th 7:30 am Breakfast (service ends at 8:45 am) 9:00 am Session 1: Target selection and guidance Chair: Carla Shatz 9:00 am Greg Bashaw, University of Pennsylvania Molecular mechanisms regulating midline axon guidance 9:25 am Carol A. Mason, Columbia University Patterning the visual pathways from eye to brain: Genes and rules for retinal axon decussation, organization, and targeting 9:50 am Iris Salecker, National Institute for Medical Research Layer-specific positional guidance cues control targeting of photoreceptor axons in Drosophila 10:15 am Break 10:45 am Session 2: Target selection and guidance (cont'd) Chair: Sam Pfaff 10:45 am Hitoshi Sakano, University of Tokyo Neural circuit formation in the mouse olfactory system 11:10 am Alex L. Kolodkin, HHMI/Johns Hopkins University Molecular cues that regulate neural connectivity in the developing mammalian visual system 11:35 am Andrew D. Huberman, University of California, San Diego Mechanisms that generate functional specificity of visual circuits 12:00 pm Jean-François Cloutier, Montreal Neurological Institute Improper glomerular development in the accessory olfactory bulb of kirrel-3 mutant mice is associated with a loss of male-male aggression 12:25 pm Lunch (service ends at 1:00 pm) updated 03/26/12 2 Janelia Farm Conference: Constructing Neural Circuits, April 29-May 2, 2012 2:00 pm Session 3: RNA-based guidance mechanisms Chair: David Ginty 2:00 pm Robert Darnell, HHMI/Rockefeller University Mapping RNA control of neural circuits 2:25 pm John G.
    [Show full text]
  • Biphasic Functions for the GDNF-Ret Signaling Pathway in Chemosensory Neuron Development and Diversification
    Biphasic functions for the GDNF-Ret signaling pathway in chemosensory neuron development and diversification Christopher R. Donnellya, Amol A. Shaha, Charlotte M. Mistrettaa, Robert M. Bradleya, and Brian A. Pierchalaa,1 aDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109 Edited by Solomon H. Snyder, Johns Hopkins University School of Medicine, Baltimore, MD, and approved November 21, 2017 (received for review May 27, 2017) The development of the taste system relies on the coordinated on the soft palate as well as somatosensory neurons projecting to regulation of cues that direct the simultaneous development of the external ear (8). This major ganglion for orofacial sensation both peripheral taste organs and innervating sensory ganglia, but is complex and multimodal, with soma for taste, touch, and lin- the underlying mechanisms remain poorly understood. In this study, gual temperature reception (5–7). However, our knowledge of we describe a novel, biphasic function for glial cell line-derived the molecular mechanisms dictating GG chemosensory neuron neurotrophic factor (GDNF) in the development and subsequent development and postnatal heterogeneity remain poorly under- diversification of chemosensory neurons within the geniculate stood, especially compared with primary sensory afferent neurons in ganglion (GG). GDNF, acting through the receptor tyrosine kinase the DRG and TG. Ret, regulates the expression of the chemosensory fate determinant Previous studies have focused on the role of the neurotrophins Ret−/− Retfx/fx Phox2b early in GG development. mice, but not ; in chemosensory GG development and maintenance, with NT-4 Phox2b -Cre mice, display a profound loss of Phox2b expression with and BDNF emerging as the principal regulators of GG axon guid- subsequent chemosensory innervation deficits, indicating that Ret is ance (9), GG neuron survival (10), and CT nerve regeneration (11).
    [Show full text]