Comprehensive Transcriptome Data for Endemic Schizothoracinae Fish In
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The Status of the Endangered Freshwater Fishes in China and the Analysis of the Endangered Causes Institute of Hydrobiology
The status of the endangered freshwater fishes in China and The analysis of the endangered causes HE Shunping, CIIEN Yiyu Institute of Hydrobiology, CAS, Wuhan, ITubei Province, 430072 Abstract More than 800 species of freshwater fishes are precious biological resources in inland water system of China. Among them, there are a great number of endemic and precious group, and a lot of monotypic genera and species. Recently, owing to the synthetic effects of the natural and human-beings, many of these fishes gradually became endangered. The preliminary statistic result indicates that 92 species are endangered fishes and account for 10% of the total freshwater fishes in China. For the purpose of protection of the biodiversity of fishes, it is necessary to analyse these causes which have led the fishes to become endangered. This report could be used as a scientific reference for researching and saving the endemic precious freshwater fishes in China. Key words Endangered freshwater fishes, Endangered causes, China In the process of the evolution of living things, along with the origin of life, the extinction of life also existed. In the long_ life history, the speciation and the extinction of living things often keep a relative balance. As time goes on, especially after by the impact of human beings activity of production and life, the pattern of the biodiversity were changed or damaged, more or less. At last, in the modern society, human beings activity not only accelerate the progress of society and the development of economy, but also, as a special species, become the source of disturbing_ to other species. -
Duplication of Toll-Like Receptor 22 in Teleost Fishes
Fish and Shellfish Immunology 94 (2019) 752–760 Contents lists available at ScienceDirect Fish and Shellfish Immunology journal homepage: www.elsevier.com/locate/fsi Full length article Duplication of toll-like receptor 22 in teleost fishes T ∗ Delin Qia, , Yan Chaob, Cunfang Zhanga, Zhenji Wangc, Wei Wanga, Qichang Chena, Ziqin Zhenga,b, Zhao Zhanga,b a State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, China b Animal Science Department of Agriculture and Animal Husbandry College, Qinghai University, Xining, 810016, China c Fishery Environmental Monitoring Station of Qinghai Province, Xining, 810012, China ARTICLE INFO ABSTRACT Keywords: The TLRs of teleost fishes have distinct features and are highly diverse, but the duplication characteristics and Toll-like receptor 22 expression patterns of the tlr22 gene remain unclear. Here, we identified paralogous tlr22 genes in 13 teleost Duplication fishes by screening available fish genomic resources and using molecular cloning. We then conducted com- Expression pattern prehensive bioinformatics analyses and investigated spatiotemporal differences in the expression patterns of the Gymnocypris eckloni tlr22 genes in G. eckloni. The results indicated that more than three paralogous tlr22 genes were possessed by Teleost fishes some teleost fishes. Of these, tlr22c is specific to some subfamilies of the Cyprinidae (e.g., Barbinae, Cyprininae, Schizothoracinae, and Leuciscinae). Phylogenetic and syntenic analyses showed that the paralogous tlr22 genes originated from two single-gene duplication events. Molecular clock calculations dated the two gene duplication events at 49.5 and 39.3 MYA, which is before the common carp-specific genome duplication event and well after the fish-specific genome duplication. -
A REVIEW of the STATUS and THREATS to WETLANDS in NEPAL Re! on the Occasion Of3 I UCN World Conservation Congress, 2004
A REVIEW OF THE STATUS AND THREATS TO WETLANDS IN NEPAL re! On the occasion of3 I UCN World Conservation Congress, 2004 A REVIEW OF THE STATUS AND THREATS TO WETLANDS IN NEPAL IUCN Nepal 2004 IUCN The World Conservation Union IUCN The World Conservation Union The support of UNDP-GEF to IUCN Nepal for the studies and design of the national project on Wetland Conservation and Sustainable Use and the publication of this document is gratefully acknowledged. Copyright: © 2004 IUCN Nepal Published June 2004 by IUCN Nepal Country Office Reproduction of this publication for educational or other non-commercial purposes is authorised without prior written permission from the copyright holder provided the source is fully acknowledged. Reproduction of this publication for resale or other commercial purposes is prohibited without prior written permission of the copyright holder. Citation: IUCN Nepal (2004). A Review o(the Status andThreats to Wetlands in Nepal 78+v pp. ISBN: 99933-760-9-4 Editing: Sameer Karki and Samuel Thomas Cover photo: Sanchit Lamichhane Design & Layout: WordScape, Kathmandu Printed by: Jagadamba Press, Hattiban, Lalitpur Available from: IUCN Nepal, P.O. Box 3923, Kathmandu, Nepal Tel: (977-1) 5528781,5528761,5526391, Fax:(977-I) 5536786 email: [email protected], URL: http://www.iucnnepal.org Foreword This document is the result of a significant project development effort undertaken by the IUCN Nepal Country Office over the last two years, which was to design a national project for conservation and sustainable use of wetlands in the country.This design phase was enabled by a UNDP-GEF PDF grant. -
6 Days Lhasa Namtso Lake Tour
www.lilysunchinatours.com 6 Days Lhasa Namtso Lake Tour Basics Tour Code: LCT - Tibet - 6D - 01 Attractions: Potala Palace, Jokhang Temple, Barkhor Street, Norbulingka, Drepung Monastery, Sera Monastery, Namtso Lake Overview: Lhasa is not just about history and Buddhism; it’s also about the scenery and landscape. This specially designed itinerary combines both the city highlights of Lhasa but the picture-postcard Namtso Lake. The exotic Buddhist monasteries in Lhasa city will give you a sense of what modern Tibetans look like, while the beautiful yet harsh landscape of the holy Namtso Lake will tell you about the Tibetan nomads. Join us for a once-in-a-lifetime journey. Highlights Shower in the warm sunshine, azure blue sky and cool weather of Lhasa city; Be moved by the devoutness of the Tibetan Buddhist pilgrims; Taste the exotic Tibetan culture in the world-famous Barkhor Street; Marvel at the stunning scenery of Namtso Lake; Immerse yourself in the vigorous debates over Buddhism. Itinerary Date Starting Time Destination Day 1 Flexible Arrival in Lhasa Day 2 9:00 a.m Potala Palace, Jokhang Temple, Barkhor Street Day 3 9:00 a.m Drepung Monastery, Sera Monastery Day 4 7:00 a.m Drive to Namtso Lake, Overnight in a Namtso Guesthouse Tel: +86 18629295068 1 Email: [email protected]; [email protected] www.lilysunchinatours.com Day 5 9:00 a.m Namtso - Lhasa Day 6 Flexible Lhasa Departure Day 1: Arrival in Lhasa Upon your arrival in Lhasa, either by train or plane, our Lhasa guide and driver will pick you up and transfer you to your hotel. -
附录1 甘肃省鱼类种类组成appendix 1 Fish Species Composition in Gansu
王太, 张艳萍, 管丽红, 杜岩岩, 娄忠玉, 焦文龙. 甘肃省鱼类资源现状及DNA条形码在鱼类物种鉴定中的应用. 生物多样性, 2015, 23(3): 306-313. http://www.biodiversity-science.net/CN/article/downloadArticleFile.do?attachType=PDF&id=10015 附录1 甘肃省鱼类种类组成 Appendix 1 Fish species composition in Gansu Province 目 Order 科 Family 属 Genus 种 Species 鲑形目 Salmoniformes 鲑科 Salmonidae 细鳞鲑属 Brachymystax 秦岭细鳞鲑 Brachymystax lenok tsinlingensis 公鱼属 Hypomesus 池沼公鱼 Hypomesus olidus 鲇形目 Siluriformes 鲇科 Siluridae 鲇属 Silurus 鲇鱼 Silurus asotus 怀头鲶 Silurus soldatovi 鮡科 Sisoridae 鮡属 Pareuchiloglanis 前臀鮡 Pareuchiloglanis anteanalis 纹胸鮡属 Glyptothorax 中华纹胸鮡 Glyptothorax sinense 鲿科 Bagridae 拟鲿属 Pseudobagrus 切尾拟鲿 Pseudobagrus truncatus 鱯属 Mystus 大鳍鱯 Mystus macropterus 鲈形目 Perciformes 鰕虎鱼科 Gobiidae 吻鰕虎鱼属 Rhinogobius 褐吻鰕虎鱼 Rhinogobius brunneus 塘鳢科 Hypseleotris 黄黝鱼属 Hypseleotris 黄黝鱼 Hypseleotris swinhonis 合鳃鱼目 Symbranchiformes 合鳃鱼科 Synbranchidae 黄鳝属 Monopterus 黄鳝 Monopterus albus 鲤形目 Cypriniformes 鲤科 Cyprinidae 鰟鮍属 Rhodeus 高体鰟鮍 Rhodeus ocellatus 棒花鱼属 Abbottina 棒花鱼 Abbottina rivularis 颌须鮈属 Gnathopogon 嘉陵颌须鮈 Gnathopogon herzensteini 短须颌须鮈 Gnathopogon imberbis 胡鮈属 Huigobio 清徐胡鮈 Huigobio chinssuensis 鮈属 Gobio 黄河鮈 Gobio huanghensis 似铜鮈 Gobio coriparoides 蛇鮈属 Saurogobio 蛇鮈 Saurogobio dabryi 似鮈属 Pseudogobio 似鮈 Pseudogobio vaillanti 片唇鮈属 Platysmacheilus 裸腹片唇鮈 Platysmacheilus nudiventris 麦穗鱼属 Pseudorasbora 麦穗鱼 Pseudorasbora parva 属 Hemibarbus 唇 Hemibarbus labeo 花 Hemibarbus maculatus 似 属 Belligobio 似 Belligobio nummifer 马口鱼属 Opsariichthys 马口鱼 Opsariichthys bidens 鱲属 Zacco 宽鳍鱲 Zacco platypus 属 Phoxinus 拉氏 Phoxinus lagowskii 尖头 Phoxinus -
De Novo Assembly of Schizothorax Waltoni Transcriptome to Identify Immune-Related Genes Cite This: RSC Adv.,2018,8, 13945 and Microsatellite Markers†
RSC Advances View Article Online PAPER View Journal | View Issue De novo assembly of Schizothorax waltoni transcriptome to identify immune-related genes Cite this: RSC Adv.,2018,8, 13945 and microsatellite markers† Hua Ye,ab Zhengshi Zhang,ab Chaowei Zhou,ab Chengke Zhu,ab Yuejing Yang,ab Mengbin Xiang,ab Xinghua Zhou,ab Jian Zhou*c and Hui Luo *ab Schizothorax waltoni (S. waltoni) is one kind of the subfamily Schizothoracinae and an indigenous economic tetraploid fish to Tibet in China. It is rated as a vulnerable species in the Red List of China's Vertebrates, owing to overexploitation and biological invasion. S. waltoni plays an important role in ecology and local fishery economy, but little information is known about genetic diversity, local adaptation, immune system and so on. Functional gene identification and molecular marker development are the first and essential step for the following biological function and genetics studies. For this purpose, the transcriptome from pooled tissues of three adult S. waltoni was sequenced and Creative Commons Attribution-NonCommercial 3.0 Unported Licence. analyzed. Using paired-end reads from the Illumina Hiseq4000 platform, 83 103 transcripts with an N50 length of 2337 bp were assembled, which could be further clustered into 66 975 unigenes with an N50 length of 2087 bp. The majority of the unigenes (58 934, 87.99%) were successfully annotated by 7 public databases, and 15 KEGG pathways of immune-related genes were identified for the following functional research. Furthermore, 19 497 putative simple sequence repeats (SSRs) of 1–6 bp unit length were detected from 14 690 unigenes (21.93%) with an average distribution density of 1 : 3.28 kb. -
Sichuan/Gansu/Qinghai/Tibet (14 Days) We Love Road Journeys
Tibetan Highlands: Sichuan/Gansu/Qinghai/Tibet (14 Days) We love road journeys. They are by far our favourite way of traveling. We think the world of western China and the countries that border on this region – think Vietnam, Lao, Thailand, Myanmar, for example. On the Road Experiences is all about sharing with like-minded travelers just how beautiful a road journey in these varied lands can be. Now turn the page to find out what we’ve come to love so much… p2 p3 Itinerary Map …where you will travel… p. 006 Yes, it is possible… p. 008 Journey of Discovery… p. 010 Day-by-day… p. 056 In closing... Any car you like, so long as it is an SUV… p. 077 Adventures and discoveries in local cuisines p. 078 What’s included/Best Months to Go... p. 080 Photo credits p. 083 p5 Itinerary Map Day1 Day8 Arrival in Chengdu – Dulan to Golmud – Apply for your temporary driving Across the Qaidam Basin to Golmud license and visit Chengdu’s beautiful Panda Reserve Day9 Golmud to Tuotuohe – Day2 Up, up, up - Onto the Plateau and Chengdu to Maerkang – into the highlands of Qinghai Through the valleys to the Gyarong Tibetan region Day10 Tuotuohe to Naqu – Day3 Cross the famous Tanggula Pass on Maerkang to Ruoergai – your way to Tibet itself Towards the very north of Sichuan on the way to Gansu Day11 Naqu to Damxung – Day4 Visit one of Tibet’s holiest lakes, Ruoergai to Xiahe – Lake Nam-tso Your first and only stop in Gansu province Day12 Damxung to Lhasa – Day5 Complete your journey with Xiahe to Qinghai’s capital, Xining – a beautiful drive to your final On your way to Qinghai destination Day6 Day13 Xining – In and around Lhasa – Spend a day in and around Xining for Visit Potala Palace and explore the a bit of rest and visit the spectacular old city of Lhasa Ta’er Monastery Day14 Day7 Depart from Lhasa – Xining to Dulan – Lift must go on...Farewell Lhasa On the way to Golmud.. -
Phylogeography of Schizopygopsis Stoliczkai (Cyprinidae) in Northwest Tibetan Plateau Area
Received: 11 April 2017 | Revised: 14 August 2017 | Accepted: 17 August 2017 DOI: 10.1002/ece3.3452 ORIGINAL RESEARCH Phylogeography of Schizopygopsis stoliczkai (Cyprinidae) in Northwest Tibetan Plateau area Kunyuan Wanghe1,2,3,4 | Yongtao Tang1,2,3,4 | Fei Tian1,2,4 | Chenguang Feng1,2,3,4 | Renyi Zhang5 | Guogang Li6 | Sijia Liu1,2,3,4 | Kai Zhao1,2,4 1Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Abstract Plateau Biology, Chinese Academy of Schizopygopsis stoliczkai (Cyprinidae, subfamily Schizothoracinae) is one of the major Sciences, Xining, Qinghai, China freshwater fishes endemic to the northwestern margin of the Tibetan Plateau. In the 2Laboratory of Plateau Fish Evolutionary and Functional Genomics, Northwest Institute current study, we used mitochondrial DNA markers cytochrome b (Cyt b) and 16S of Plateau Biology, Chinese Academy of rRNA (16S), as well as the nuclear marker, the second intron of the nuclear beta- actin Sciences, Xining, Qinghai, China gene (Act2), to uncover the phylogeography of S. stoliczkai. In total, we obtained 74 3Qinghai Key Laboratory of Animal Ecological Genomics, Xining, Qinghai, China haplotypes from 403 mitochondrial concatenated sequences. The mtDNA markers 4University of Chinese Academy of Sciences, depict the phylogenetic structures of S. stoliczkai, which consist of clade North and Beijing, China clade South. The split time of the two clades is dated back to 4.27 Mya (95% 5Guizhou Normal University, Guiyang, China HPD = 1.96–8.20 Mya). The estimated split time is earlier than the beginning of the 6Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, ice age of Pleistocene (2.60 Mya), suggesting that the northwestern area of the Tibetan Mengla, China Plateau probably contain at least two glacial refugia for S. -
From Kunlun Pass Basin, Northeastern Tibetan Plateau and Their Bearings on Development of Water System and Uplift of the Area
SCIENCE CHINA Earth Sciences • RESEARCH PAPER • April 2010 Vol.53 No.4: 485–500 doi: 10.1007/s11430-010-0048-5 Pliocene cyprinids (Cypriniformes, Teleostei) from Kunlun Pass Basin, northeastern Tibetan Plateau and their bearings on development of water system and uplift of the area WANG Ning & CHANG Mee-mann* Key Laboratory of Evolutionary Systematics of Vertebrates, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044, China Received November 23, 2009; accepted February 25, 2010 Here described are the cyprinid fossils from the Pliocene Lower Member of Qiangtang Formation of the Kunlun Pass Basin, northeastern Tibetan Plateau, collected at a locality 4769 m above the sea level (asl). The materials consist of numerous disar- ticulated and incomplete bones as well as thousands of pharyngeal teeth, fin rays, and vertebrae. The fossils were referred to the genus Gymnocypris, lineage Schizothoracini, family Cyprinidae; the lineage Schizothoracini; and the family Cyprinidae respectively. The Schizothoracini is a freshwater fish group endemic to the Tibetan Plateau and its surrounding area. Previous workers on living schizothoracins regarded that Gymnocypris belongs to the highly specialized grade of the group, colonizing higher altitudes than other members of the group. Two species are so far unequivocally assigned to the genus, i.e., G. przewalskii and G. eckloni, and they are inhabiting Qinghai Lake and the waters on both north (the Golmud River) and south (upper reach of the Yellow River) sides of the East Kunlun Mountain, respectively. The abundant fossil schizothoracins occur in the Kunlun Pass Basin on the southern slope of the East Kunlun Mountain (at 4769 m asl), close to the present Golmud River, indicating comparatively rich waters in the area and possible connections between the water systems on north and south sides of the East Kunlun Mountain during the Pliocene. -
Everest North Face Base Camp & Holy
EVEREST NORTH FACE BASE CAMP & HOLY NAMTSO LAKE Tashi delek! Tibetan Guide Travel Tours is a small travel agency based in Lhasa. We always work hard and take responsible for our clients by using local services as much as possible. Of course we use Tibetan drivers and tour guides. Who are experienced, have rich knowledge about Tibetan culture and also excellent attitude. We are confident that you would not be disappointed if you choose our services letting us show you our mother land. Proposed itinerary Day 1: Lhasa arrival [3650m] Upon arrival in Lhasa you will be welcomed by your English-speaking Tibetan Guide and Tibetan Driver who will bring you to your hotel. Acclimatization to high altitude: please, drink lots of water and take plenty of rest in order to minimize altitude sickness. Overnight at Shang Bala Hotel 4*, which is located in Lhasa city center and few walking steps to Barkhor Square and Jokhang Temple. Day 2: Lhasa sightseeing We begin visiting Ramoche Temple, built in honor of the image of Jowo Rinpoche that Chinese princess Wencheng brought by marrying Songtsen Gampo, the first king of Buddhist doctrine and who unified the Tibetan empire in the 7th century. Thereafter, we continue with Jokhang Temple, the most sacred monastery in Tibet. It was also founded in the 7th century by Songtsen Gampo. Later you can explore the surrounding Barkhor old quarter and spend time walking around Jokhang Temple following pilgrims from all over the Tibetan plateau. In the afternoon we go to Sera Monastery, one of three great universities of Gelugpa Sect. -
Teleostei: Cypriniformes: Cyprinidae) Inferred from Complete Mitochondrial Genomes
Biochemical Systematics and Ecology 64 (2016) 6e13 Contents lists available at ScienceDirect Biochemical Systematics and Ecology journal homepage: www.elsevier.com/locate/biochemsyseco Molecular phylogeny of the subfamily Schizothoracinae (Teleostei: Cypriniformes: Cyprinidae) inferred from complete mitochondrial genomes * Jie Zhang a, b, Zhuo Chen a, Chuanjiang Zhou b, Xianghui Kong b, a College of Life Science, Henan Normal University, Xinxiang 453007, PR China b College of Fisheries, Henan Normal University, Xinxiang 453007, PR China article info abstract Article history: The schizothoracine fishes, members of the Teleost order Cypriniformes, are one of the Received 16 June 2015 most diverse group of cyprinids in the QinghaieTibetan Plateau and surrounding regions. Received in revised form 19 October 2015 However, taxonomy and phylogeny of these species remain unclear. In this study, we Accepted 14 November 2015 determined the complete mitochondrial genome of Schizopygopsis malacanthus. We also Available online xxx used the newly obtained sequence, together with 31 published schizothoracine mito- chondrial genomes that represent eight schizothoracine genera and six outgroup taxa to Keywords: reconstruct the phylogenetic relationships of the subfamily Schizothoracinae by different Mitochondrial genome Phylogeny partitioned maximum likelihood and partitioned Bayesian inference at nucleotide and fi Schizothoracinae amino acid levels. The schizothoracine shes sampled form a strongly supported mono- Schizopygopsis malacanthus phyletic group that is the sister taxon to Barbus barbus. A sister group relationship between the primitive schizothoracine group and the specialized schizothoracine group þ the highly specialized schizothoracine group was supported. Moreover, members of the specialized schizothoracine group and the genera Schizothorax, Schizopygopsis, and Gym- nocypris were found to be paraphyletic. © 2015 Published by Elsevier Ltd. -
Global Tissue Transcriptomic Analysis to Improve Genome Annotation And
www.nature.com/scientificreports OPEN Global tissue transcriptomic analysis to improve genome annotation and unravel skin pigmentation in goldfsh Wu Gan1, Yu‑Wen Chung‑Davidson2, Zelin Chen3, Shiying Song1, Wenyao Cui1, Wei He1, Qinghua Zhang1,4, Weiming Li2, Mingyou Li1* & Jianfeng Ren1,4* Goldfsh is an ornamental fsh with diverse phenotypes. However, the limited genomic resources of goldfsh hamper our understanding of the genetic basis for its phenotypic diversity. To provide enriched genomic resources and infer possible mechanisms underlying skin pigmentation, we performed a large‑scale transcriptomic sequencing on 13 adult goldfsh tissues, larvae at one‑ and three‑days post hatch, and skin tissues with four diferent color pigmentation. A total of 25.52 Gb and 149.80 Gb clean data were obtained using the PacBio and Illumina platforms, respectively. Onto the goldfsh reference genome, we mapped 137,674 non‑redundant transcripts, of which 5.54% was known isoforms and 78.53% was novel isoforms of the reference genes, and the remaining 21,926 isoforms are novel isoforms of additional new genes. Both skin‑specifc and color‑specifc transcriptomic analyses showed that several signifcantly enriched genes were known to be involved in melanogenesis, tyrosine metabolism, PPAR signaling pathway, folate biosynthesis metabolism and so on. Thirteen diferentially expressed genes across diferent color skins were associated with melanogenesis and pteridine synthesis including mitf, ednrb, mc1r, tyr, mlph and gch1, and xanthophore diferentiation such as pax7, slc2a11 and slc2a15. These transcriptomic data revealed pathways involved in goldfsh pigmentation and improved the gene annotation of the reference genome. Goldfsh (Carassius auratus auratus) is an important ornamental fsh, and has been subjected to extremely intensive artifcial selection during its domestication history1,2.