RESEARCH ARTICLE A New Species of Cleisostoma (Orchidaceae) from the Hon Ba Nature Reserve in Vietnam: A Multidisciplinary Assessment Jan Ponert1,2,3*, Pavel Trávníček3,4, Truong Ba Vuong5, Romana Rybková1, Jan Suda3,4 1 Prague Botanical Garden, Prague, Czech Republic, 2 Department of Experimental Plant Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic, 3 Department of Botany, Faculty of Science, Charles University in Prague, Prague, Czech Republic, 4 Institute of Botany, The Czech Academy of Sciences, Průhonice, Czech Republic, 5 Institute of Tropical Biology, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam *
[email protected] Abstract OPEN ACCESS A new species, Cleisostoma yersinii J. Ponert & Vuong, is described and illustrated based Citation: Ponert J, Trávníček P, Vuong TB, Rybková on the material collected in the Hon Ba Nature Reserve in southern Vietnam. In addition to R, Suda J (2016) A New Species of Cleisostoma conventional (macro)morphological examination we comparatively investigated root and (Orchidaceae) from the Hon Ba Nature Reserve in leaf anatomy (using light and fluorescent microscopy), assessed nectar characteristics Vietnam: A Multidisciplinary Assessment. PLoS ONE 11(3): e0150631. doi:10.1371/journal.pone.0150631 (using HPLC analysis), determined nuclear genome size (using DNA flow cytometry) and reconstructed phylogenetic relationships (using nrITS sequences). Cleisostoma yersinii dif- Editor: Zhong-Jian Liu, The National Orchid Conservation Center of China; The Orchid fers from its putative closest relative C. birmanicum in wider and shorter leaves, larger flow- Conservation & Research Center of Shenzhen, ers, distinct lip with S-shaped tip of the mid-lobe, and a shallow spur with two large nectar CHINA sacks separated by prominent calli and septum.