21.10.2014CNOT4 antibody (pAb)

Rabbit Anti-Human/Mouse/Rat CCR4-NOT Transcription Complex Subunit 4 (NOT4, NOT4H)

Instruction Manual

Catalog Number PK-AB718-4813 Synonyms CNOT4 Antibody: CCR4-NOT transcription complex subunit 4, NOT4, NOT4H Description CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist. Quantity 100 µg Source / Host Rabbit Immunogen CNOT4 antibody was raised against a 19 amino acid peptide near the amino terminus of the human CNOT4. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. CNOT4 antibody can be used for detection of CNOT4 by Western blot at 1 - 2 µg/mL. Images Available upon request. References Albert TK, Hanzawa H, Legtenberg IA, et al. Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex. EMBO J. 2002; 21:355-64. Winkler GS, Albert TK, Dominguez C, et al. An altered specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair. J. Mol. Biol. 2004; 337:157-65. Mulder KW, Inagaki A, Cameroni E, et al. Modulation of Ubc4p/Ubc5p-mediated stress responses by the RING-finger-dependent ubiquitin-protein ligase Not4p in Saccharomyces cerevisiae. Genetics 2007; 176:181-92.

Images Available upon request. Related Products Cat.No. PK-AB718-4813P - CNOT4 Peptide Cat. No. PK-AB718-1203 - A549 Cell Lysate

FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014