Representatives of a Novel Archaeal Phylum Or a Fast-Evolving
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Extremozymes of the Hot and Salty Halothermothrix Orenii
Extremozymes of the Hot and Salty Halothermothrix orenii Author Kori, Lokesh D Published 2012 Thesis Type Thesis (PhD Doctorate) School School of Biomolecular and Physical Sciences DOI https://doi.org/10.25904/1912/2191 Copyright Statement The author owns the copyright in this thesis, unless stated otherwise. Downloaded from http://hdl.handle.net/10072/366220 Griffith Research Online https://research-repository.griffith.edu.au Extremozymes of the hot and salty Halothermothrix orenii LOKESH D. KORI (M.Sc. Biotechnology) School of Biomolecular and Physical Sciences Science, Environment, Engineering and Technology Griffith University, Australia Submitted in fulfillment of the requirements of the degree of Doctor of Philosophy December 2011 STATEMENT OF ORIGINALITY STATEMENT OF ORIGINALITY This work has not previously been submitted for a degree or diploma in any university. To the best of my knowledge and belief, the thesis contains no material previously published or written by another person except where due reference is made in the thesis itself. LOKESH DULICHAND KORI II ACKNOWLEDGEMENTS ACKNOWLEDGEMENTS I owe my deepest gratitude to my supervisor Prof. Bharat Patel, for offering me an opportunity for being his postgraduate. His boundless knowledge motivates me for keep going and enjoy the essence of science. Without his guidance, great patience and advice, I could not finish my PhD program successfully. I take this opportunity to give my heartiest thanks to Assoc. Prof. Andreas Hofmann, (Structural Chemistry, Eskitis Institute for Cell & Molecular Therapies, Griffith University) for his support and encouragement for crystallographic work. I am grateful to him for teaching me about the protein structures, in silico analysis and their hidden chemistry. -
The Status of Natural Resources on the High-Seas
The status of natural resources on the high-seas Part 1: An environmental perspective Part 2: Legal and political considerations An independent study conducted by: The Southampton Oceanography Centre & Dr. A. Charlotte de Fontaubert The status of natural resources on the high-seas i The status of natural resources on the high-seas Published May 2001 by WWF-World Wide Fund for Nature (Formerly World Wildlife Fund) Gland, Switzerland. Any reproduction in full or in part of this publication must mention the title and credit the above mentioned publisher as the copyright owner. The designation of geographical entities in this book, and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of WWF or IUCN concerning the legal status of any country, territory, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. The views expressed in this publication do not necessarily reflect those of WWF or IUCN. Published by: WWF International, Gland, Switzerland IUCN, Gland, Switzerland and Cambridge, UK. Copyright: © text 2001 WWF © 2000 International Union for Conservation of Nature and Natural Resources © All photographs copyright Southampton Oceanography Centre Reproduction of this publication for educational or other non-commercial purposes is authorized without prior written permission from the copyright holder provided the source is fully acknowledged. Reproduction of this publication for resale or other commercial purposes is prohibited without prior written permission of the copyright holder. Citation: WWF/IUCN (2001). The status of natural resources on the high-seas. WWF/IUCN, Gland, Switzerland. Baker, C.M., Bett, B.J., Billett, D.S.M and Rogers, A.D. -
Thermodynamics of DNA Binding by DNA Polymerase I and Reca
Louisiana State University LSU Digital Commons LSU Doctoral Dissertations Graduate School 2014 Thermodynamics of DNA Binding by DNA Polymerase I and RecA Recombinase from Deinococcus radiodurans Jaycob Dalton Warfel Louisiana State University and Agricultural and Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_dissertations Recommended Citation Warfel, Jaycob Dalton, "Thermodynamics of DNA Binding by DNA Polymerase I and RecA Recombinase from Deinococcus radiodurans" (2014). LSU Doctoral Dissertations. 2382. https://digitalcommons.lsu.edu/gradschool_dissertations/2382 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Doctoral Dissertations by an authorized graduate school editor of LSU Digital Commons. For more information, please [email protected]. THERMODYNAMICS OF DNA BINDING BY DNA POLYMERASE I AND RECA RECOMBINASE FROM DEINOCOCCUS RADIODURANS A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Department of Biological Sciences by Jaycob Dalton Warfel B.S. Louisiana State University, 2006 May 2015 ACKNOWLEDGEMENTS I would like to express my utmost gratitude to the myriad of individuals who have lent their support during the time it has taken to complete this dissertation. First and foremost is due glory to God, The Father, The Son and The Holy Spirit, through whom all is accomplished. It is with extreme thankfulness for the blessings bestowed upon me, and with vast appreciation for the beauty of God’s creation that I have pursued a scientific education. -
Evidence of Active Methanogen Communities in Shallow Sediments
Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps Adrien Vigneron, St´ephaneL'Haridon, Anne Godfroy, Erwan Roussel, Barry A Cragg, R. John Parkes, Laurent Toffin To cite this version: Adrien Vigneron, St´ephaneL'Haridon, Anne Godfroy, Erwan Roussel, Barry A Cragg, et al.. Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps. Applied and Environmental Microbiology, American Society for Microbiology, 2015, 81 (10), pp.3451-3459. <10.1128/AEM.00147-15>. <hal-01144705> HAL Id: hal-01144705 http://hal.univ-brest.fr/hal-01144705 Submitted on 23 Apr 2015 HAL is a multi-disciplinary open access L'archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destin´eeau d´ep^otet `ala diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publi´esou non, lished or not. The documents may come from ´emanant des ´etablissements d'enseignement et de teaching and research institutions in France or recherche fran¸caisou ´etrangers,des laboratoires abroad, or from public or private research centers. publics ou priv´es. Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License Evidence of Active Methanogen Communities in Shallow Sediments of the Sonora Margin Cold Seeps Adrien Vigneron,a,b,c,e Stéphane L’Haridon,b,c Anne Godfroy,a,b,c Erwan G. Roussel,a,b,c,d Barry A. Cragg,d R. John Parkes,d Downloaded from Laurent Toffina,b,c Ifremer, Laboratoire de Microbiologie -
Access to Electronic Thesis
Access to Electronic Thesis Author: Khalid Salim Al-Abri Thesis title: USE OF MOLECULAR APPROACHES TO STUDY THE OCCURRENCE OF EXTREMOPHILES AND EXTREMODURES IN NON-EXTREME ENVIRONMENTS Qualification: PhD This electronic thesis is protected by the Copyright, Designs and Patents Act 1988. No reproduction is permitted without consent of the author. It is also protected by the Creative Commons Licence allowing Attributions-Non-commercial-No derivatives. If this electronic thesis has been edited by the author it will be indicated as such on the title page and in the text. USE OF MOLECULAR APPROACHES TO STUDY THE OCCURRENCE OF EXTREMOPHILES AND EXTREMODURES IN NON-EXTREME ENVIRONMENTS By Khalid Salim Al-Abri Msc., University of Sultan Qaboos, Muscat, Oman Mphil, University of Sheffield, England Thesis submitted in partial fulfillment for the requirements of the Degree of Doctor of Philosophy in the Department of Molecular Biology and Biotechnology, University of Sheffield, England 2011 Introductory Pages I DEDICATION To the memory of my father, loving mother, wife “Muneera” and son “Anas”, brothers and sisters. Introductory Pages II ACKNOWLEDGEMENTS Above all, I thank Allah for helping me in completing this project. I wish to express my thanks to my supervisor Professor Milton Wainwright, for his guidance, supervision, support, understanding and help in this project. In addition, he also stood beside me in all difficulties that faced me during study. My thanks are due to Dr. D. J. Gilmour for his co-supervision, technical assistance, his time and understanding that made some of my laboratory work easier. In the Ministry of Regional Municipalities and Water Resources, I am particularly grateful to Engineer Said Al Alawi, Director General of Health Control, for allowing me to carry out my PhD study at the University of Sheffield. -
Functionalized Membrane Domains: an Ancestral Feature of Archaea? Maxime Tourte, Philippe Schaeffer, Vincent Grossi, Phil Oger
Functionalized Membrane Domains: An Ancestral Feature of Archaea? Maxime Tourte, Philippe Schaeffer, Vincent Grossi, Phil Oger To cite this version: Maxime Tourte, Philippe Schaeffer, Vincent Grossi, Phil Oger. Functionalized Membrane Domains: An Ancestral Feature of Archaea?. Frontiers in Microbiology, Frontiers Media, 2020, 11, pp.526. 10.3389/fmicb.2020.00526. hal-02553764 HAL Id: hal-02553764 https://hal.archives-ouvertes.fr/hal-02553764 Submitted on 20 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. fmicb-11-00526 March 30, 2020 Time: 21:44 # 1 ORIGINAL RESEARCH published: 31 March 2020 doi: 10.3389/fmicb.2020.00526 Functionalized Membrane Domains: An Ancestral Feature of Archaea? Maxime Tourte1†, Philippe Schaeffer2†, Vincent Grossi3† and Phil M. Oger1*† 1 Université de Lyon, INSA Lyon, CNRS, MAP UMR 5240, Villeurbanne, France, 2 Université de Strasbourg-CNRS, UMR 7177, Laboratoire de Biogéochimie Moléculaire, Strasbourg, France, 3 Université de Lyon, ENS Lyon, CNRS, Laboratoire de Géologie de Lyon, UMR 5276, Villeurbanne, France Bacteria and Eukarya organize their plasma membrane spatially into domains of distinct functions. Due to the uniqueness of their lipids, membrane functionalization in Archaea remains a debated area. -
And Thermo-Adaptation in Hyperthermophilic Archaea: Identification of Compatible Solutes, Accumulation Profiles, and Biosynthetic Routes in Archaeoglobus Spp
Universidade Nova de Lisboa Osmo- andInstituto thermo de Tecnologia-adaptation Química e Biológica in hyperthermophilic Archaea: Subtitle Subtitle Luís Pedro Gafeira Gonçalves Osmo- and thermo-adaptation in hyperthermophilic Archaea: identification of compatible solutes, accumulation profiles, and biosynthetic routes in Archaeoglobus spp. OH OH OH CDP c c c - CMP O O - PPi O3P P CTP O O O OH OH OH OH OH OH O- C C C O P O O P i Dissertation presented to obtain the Ph.D degree in BiochemistryO O- Instituto de Tecnologia Química e Biológica | Universidade Nova de LisboaP OH O O OH OH OH Oeiras, Luís Pedro Gafeira Gonçalves January, 2008 2008 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica Osmo- and thermo-adaptation in hyperthermophilic Archaea: identification of compatible solutes, accumulation profiles, and biosynthetic routes in Archaeoglobus spp. This dissertation was presented to obtain a Ph. D. degree in Biochemistry at the Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa. By Luís Pedro Gafeira Gonçalves Supervised by Prof. Dr. Helena Santos Oeiras, January, 2008 Apoio financeiro da Fundação para a Ciência e Tecnologia (POCI 2010 – Formação Avançada para a Ciência – Medida IV.3) e FSE no âmbito do Quadro Comunitário de apoio, Bolsa de Doutoramento com a referência SFRH / BD / 5076 / 2001. ii ACKNOWNLEDGMENTS The work presented in this thesis, would not have been possible without the help, in terms of time and knowledge, of many people, to whom I am extremely grateful. Firstly and mostly, I need to thank my supervisor, Prof. Helena Santos, for her way of thinking science, her knowledge, her rigorous criticism, and her commitment to science. -
Open Dissertation 4-7-09.Pdf
The Pennsylvania State University The Graduate School Department of Civil and Environmental Engineering IMPROVED ANAEROBIC DIGESTER STABILITY TO ORGANIC LOADING RATE SHOCKS WITH THE USE OF AN ENVIRONMENTALLY DERIVED INOCULUM A Dissertation in Environmental Engineering and Biogeochemistry by Lisa Marie Steinberg © 2009 Lisa Marie Steinberg Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy May 2009 The dissertation of Lisa Marie Steinberg was reviewed and approved* by the following: John Regan Associate Professor of Environmental Engineering Dissertation Advisor Chair of Committee Christopher House Associate Professor of Geosciences Bruce Logan Kappe Professor of Environmental Engineering Jenn Macalady Assistant Professor of Geosciences Peggy Johnson Department Head and Professor of Civil Engineering *Signatures are on file in the Graduate School ABSTRACT Anaerobic digestion broadly describes technology that utilizes microorganisms to break down organic matter under anaerobic conditions through the coordinated efforts of several trophic groups of microorganisms. The last step is catalyzed by methanogens which produce primarily methane, carbon dioxide, and water as products of metabolism. Anaerobic digestion occurs naturally in a variety of water-saturated sediments, but is also used to treat waste in constructed reactors. There are a number of advantages to treating waste with anaerobic digestion, but perhaps the greatest is that waste treatment can be coupled to energy generation by the production of a methane-rich biogas. Despite the advantages, anaerobic digestion is severely under-utilized in waste treatment mainly due to the belief that anaerobic digesters are less stable than aerobic treatment processes. Anaerobic digesters are typically operated under warm temperatures and circumneutral pH, with operation outside of these conditions leading to instability and potential reactor failure. -
Virus World As an Evolutionary Network of Viruses and Capsidless Selfish Elements
Virus World as an Evolutionary Network of Viruses and Capsidless Selfish Elements Koonin, E. V., & Dolja, V. V. (2014). Virus World as an Evolutionary Network of Viruses and Capsidless Selfish Elements. Microbiology and Molecular Biology Reviews, 78(2), 278-303. doi:10.1128/MMBR.00049-13 10.1128/MMBR.00049-13 American Society for Microbiology Version of Record http://cdss.library.oregonstate.edu/sa-termsofuse Virus World as an Evolutionary Network of Viruses and Capsidless Selfish Elements Eugene V. Koonin,a Valerian V. Doljab National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, USAa; Department of Botany and Plant Pathology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon, USAb Downloaded from SUMMARY ..................................................................................................................................................278 INTRODUCTION ............................................................................................................................................278 PREVALENCE OF REPLICATION SYSTEM COMPONENTS COMPARED TO CAPSID PROTEINS AMONG VIRUS HALLMARK GENES.......................279 CLASSIFICATION OF VIRUSES BY REPLICATION-EXPRESSION STRATEGY: TYPICAL VIRUSES AND CAPSIDLESS FORMS ................................279 EVOLUTIONARY RELATIONSHIPS BETWEEN VIRUSES AND CAPSIDLESS VIRUS-LIKE GENETIC ELEMENTS ..............................................280 Capsidless Derivatives of Positive-Strand RNA Viruses....................................................................................................280 -
In Vivo Interactions of Archaeal Cdc6 Orc1 and Minichromosome
In vivo interactions of archaeal Cdc6͞Orc1 and minichromosome maintenance proteins with the replication origin Fujihiko Matsunaga*, Patrick Forterre*, Yoshizumi Ishino†, and Hannu Myllykallio*§ *Institut de Ge´ne´ tique et Microbiologie, Universite´de Paris-Sud, 91405 Orsay, France; and †Department of Molecular Biology, Biomolecular Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan Communicated by Bruce W. Stillman, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, July 25, 2001 (received for review May 25, 2001) Although genome analyses have suggested parallels between basis of asymmetry in base composition between leading and archaeal and eukaryotic replication systems, little is known about lagging strands (10, 11). In Pyrococcus abyssi, the location of the the DNA replication mechanism in Archaea. By two-dimensional predicted origin coincides with an early replicating chromosomal gel electrophoreses we positioned a replication origin (oriC) within segment of 80 kb identified by radioactive labeling of chromo- 1 kb in the chromosomal DNA of Pyrococcus abyssi, an anaerobic somal DNA in cultures released from a replication block (12). hyperthermophile, and demonstrated that the oriC is physically Our in silico and labeling data also allowed us to conclude that linked to the cdc6 gene. Our chromatin immunoprecipitation as- the hyperthermophilic archaeon P. abyssi uses a single bidirec- says indicated that P. abyssi Cdc6 and minichromosome mainte- tional origin to replicate its genome. We proposed that this origin nance (MCM) proteins bind preferentially to the oriC region in the would correspond to the long intergenic region conserved in all exponentially growing cells. Whereas the oriC association of MCM three known Pyrococcus sp. -
Presence of Archaea in the Indoor Environment and Their Relationships with Housing Characteristics
Microb Ecol (2016) 72:305–312 DOI 10.1007/s00248-016-0767-z ENVIRONMENTAL MICROBIOLOGY Presence of Archaea in the Indoor Environment and Their Relationships with Housing Characteristics Sepideh Pakpour1,2 & James A. Scott3 & Stuart E. Turvey 4,5 & Jeffrey R. Brook3 & Timothy K. Takaro6 & Malcolm R. Sears7 & John Klironomos1 Received: 3 April 2016 /Accepted: 5 April 2016 /Published online: 20 April 2016 # Springer Science+Business Media New York 2016 Abstract Archaea are widespread and abundant in soils, Based on the results, Archaea are not equally distributed with- oceans, or human and animal gastrointestinal (GI) tracts. in houses, and the areas with greater input of outdoor However, very little is known about the presence of Archaea microbiome and higher traffic and material heterogeneity tend in indoor environments and factors that can regulate their to have a higher abundance of Archaea. Nevertheless, more re- abundances. Using a quantitative PCR approach, and search is needed to better understand causes and consequences of targeting the archaeal and bacterial 16S rRNA genes in floor this microbial group in indoor environments. dust samples, we found that Archaea are a common part of the indoor microbiota, 5.01 ± 0.14 (log 16S rRNA gene copies/g Keywords Archaea . Bacteria . Indoor environment . qPCR . dust, mean ± SE) in bedrooms and 5.58 ± 0.13 in common Building characteristics . Human activities rooms, such as living rooms. Their abundance, however, was lower than bacteria: 9.20 ± 0.32 and 9.17 ± 0.32 in bed- rooms and common rooms, respectively. In addition, by mea- Introduction suring a broad array of environmental factors, we obtained preliminary insights into how the abundance of total archaeal The biology and ecology of the third domain of life, Archaea, 16S rRNA gene copies in indoor environment would be asso- have been studied far less when compared to the other do- ciated with building characteristics and occupants’ activities. -
Evidence for Associated Horizontal Gene Transfer in Pyrococcus Furiosus
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Digital.CSIC J Appl Genet 50(4), 2009, pp. 421–430 Original article CRISPR elements in the Thermococcales: evidence for associated horizontal gene transfer in Pyrococcus furiosus M.C. Portillo, J.M. Gonzalez Institute for Natural Resources and Agrobiology, IRNAS-CSIC, Sevilla, Spain Abstract. The presence and distribution of CRISPR (clustered regularly interspaced short palindrome repeat)el- ements in the archaeal order Thermococcales were analyzed. Four complete genome sequences from the species Pyrococcus abyssi, P. furiosus, P. horikoshii, and Thermococcus kodakaraensis were studied. A fragment of the genome of P. furiosus was flanked by CRISPR elements upstream and by a single element downstream. The composition of the gene sequences contained in this genome fragment (positions 699013 to 855319) showed sig- nificant differences from the other genes in the P. furiosus genome. Differences were observed in the GC content at the third codon positions and the frequency of codon usage between the genes located in the analyzed fragment and the other genes in the P. furiosus genome. These results represent the first evidence suggesting that repeated CRISPR elements can be involved in horizontal gene transfer and genomic differentiation of hyperthermophilic Archaea. Keywords: CRISPR, gene transfer, genome, hyperthermophilic Archaea, Pyrococcus furiosus. Introduction spacers and repetitive elements modified the phage-resistance phenotype of bacteria Genomic regions consisting of tandem repeat (Barrangou et al. 2007). The incorporation of short DNA elements, typically of 21–47 base pairs (bp) fragments of foreign DNA into the genome of in length, separated by nonrepetitive spacer se- prokaryotes at CRISPR loci has been reported quences of approximately similar length, have (Bolotin et al.