DOCSLIB.ORG

Genomic and in Situ Analyses Reveal the Micropruina Spp. As Abundant Fermentative Glycogen Accumulating Organisms in Enhanced Biological Phosphorus Removal Systems

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Genomic and in Situ Analyses Reveal the Micropruina Spp. As Abundant Fermentative Glycogen Accumulating Organisms in Enhanced Biological Phosphorus Removal Systems This may be the author’s version of a work that was submitted/accepted for publication in the following source: McIlroy, SJ, Onetto, CA, McIlroy, B, Herbst, FA, Dueholm, MS, Kirkegaard, RH, Fernando, Warnakulasuriya Eustace Yrosh, Karst, SM, Nierychlo, M, Kristensen, JM, Eales, KL, Grbin, PR, Wimmer, R, & Nielsen, PH (2018) Genomic and in Situ Analyses Reveal the Micropruina spp. as Abundant Fermentative Glycogen Accumulating Organisms in Enhanced Biological Phosphorus Removal Systems. Frontiers in Microbiology, 9, Article number: 1004. This file was downloaded from: https://eprints.qut.edu.au/204131/ c 2018 The Author(s) This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the docu- ment is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recog- nise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to [email protected] License: Creative Commons: Attribution 4.0 Notice: Please note that this document may not be the Version of Record (i.e. published version) of the work. Author manuscript versions (as Sub- mitted for peer review or as Accepted for publication after peer review) can be identified by an absence of publisher branding and/or typeset appear- ance. If there is any doubt, please refer to the published source. https://doi.org/10.3389/fmicb.2018.01004 fmicb-09-01004 May 19, 2018 Time: 14:41 # 1 ORIGINAL RESEARCH published: 23 May 2018 doi: 10.3389/fmicb.2018.01004 Genomic and in Situ Analyses Reveal the Micropruina spp. as Abundant Fermentative Glycogen Accumulating Organisms in Enhanced Biological Phosphorus Removal Systems Simon J. McIlroy1, Cristobal A. Onetto2, Bianca McIlroy1, Florian-Alexander Herbst1, Morten S. Dueholm1, Rasmus H. Kirkegaard1, Eustace Fernando1, Søren M. Karst1, Marta Nierychlo1, Jannie M. Kristensen1, Kathryn L. Eales2, Paul R. Grbin2, Reinhard Wimmer1 and Per Halkjær Nielsen1* 1 Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark, 2 School of Agriculture, Food, and Wine, The University of Adelaide, Adelaide, SA, Australia Edited by: Sabine Kleinsteuber, Helmholtz-Zentrum für Enhanced biological phosphorus removal (EBPR) involves the cycling of biomass Umweltforschung (UFZ), Germany through carbon-rich (feast) and carbon-deficient (famine) conditions, promoting the Reviewed by: Christopher Evan Lawson, activity of polyphosphate accumulating organisms (PAOs). However, several alternate University of Wisconsin–Madison, metabolic strategies, without polyphosphate storage, are possessed by other United States organisms, which can compete with the PAO for carbon at the potential expense of Ramesh K. Goel, The University of Utah, United States EBPR efficiency. The most studied are the glycogen accumulating organisms (GAOs), *Correspondence: which utilize aerobically stored glycogen to energize anaerobic substrate uptake and Per Halkjær Nielsen storage. In full-scale systems the Micropruina spp. are among the most abundant of [email protected] the proposed GAO, yet little is known about their ecophysiology. In the current study, Specialty section: genomic and metabolomic studies were performed on Micropruina glycogenica str. This article was submitted to Lg2T and compared to the in situ physiology of members of the genus in EBPR plants Microbiotechnology, Ecotoxicology and Bioremediation, using state-of-the-art single cell techniques. The Micropruina spp. were observed to a section of the journal take up carbon, including sugars and amino acids, under anaerobic conditions, which Frontiers in Microbiology were partly fermented to lactic acid, acetate, propionate, and ethanol, and partly stored Received: 05 December 2017 as glycogen for potential aerobic use. Fermentation was not directly demonstrated Accepted: 30 April 2018 Published: 23 May 2018 for the abundant members of the genus in situ, but was strongly supported by the Citation: confirmation of anaerobic uptake of carbon and glycogen storage in the absence McIlroy SJ, Onetto CA, McIlroy B, of detectable polyhydroxyalkanoates or polyphosphate reserves. This physiology is Herbst F-A, Dueholm MS, Kirkegaard RH, Fernando E, markedly different from the classical GAO model. The amount of carbon stored by Karst SM, Nierychlo M, fermentative organisms has potentially important implications for phosphorus removal – Kristensen JM, Eales KL, Grbin PR, as they compete for substrates with the Tetrasphaera PAO and stored carbon is not Wimmer R and Nielsen PH (2018) Genomic and in Situ Analyses Reveal made available to the “Candidatus Accumulibacter” PAO under anaerobic conditions. the Micropruina spp. as Abundant This study shows that the current models of the competition between PAO and GAO Fermentative Glycogen Accumulating Organisms in Enhanced Biological are too simplistic and may need to be revised to take into account the impact of potential Phosphorus Removal Systems. carbon storage by fermentative organisms. Front. Microbiol. 9:1004. doi: 10.3389/fmicb.2018.01004 Keywords: activated sludge, EPBR, fermentation, Micropruina, GAO, PAO Frontiers in Microbiology| www.frontiersin.org 1 May 2018| Volume 9| Article 1004 fmicb-09-01004 May 19, 2018 Time: 14:41 # 2 McIlroy et al. Characterization of the Micropruina GAO INTRODUCTION anaerobic carbon storage compounds are reported for full-scale activated sludge, including triacylglycerols (TAGs), gamma- Enhanced biological phosphorus removal (EBPR) activated aminobutyric acid (GABA) as well as intracellular pools of sludge systems have been widely implemented for the removal of non-polymerized fermentation by-products, long chain fatty nutrients from wastewaters. Phosphorus (P) removal is achieved acids, amino acids, and trehalose (Satoh et al., 1998; Santos in these systems by cycling of the biomass through carbon-rich et al., 1999; Kristiansen et al., 2013; McIlroy et al., 2013; (feast) anaerobic and carbon-deficient (famine) conditions Nguyen et al., 2015; Marques et al., 2017). Some organisms to encourage the activity of the so-called polyphosphate exhibiting the classical GAO and PAO phenotypes reportedly accumulating organisms (PAOs) [see Oehmen et al.(2007) for utilize sugars and amino acids directly for PHA production review]. (Liu et al., 1996; Burow et al., 2007; Oyserman et al., 2015) Classical models for the PAO phenotype stipulate that and some may also ferment glycogen stores or glucose to aerobically stored polyphosphate provides energy for lactate as an additional anaerobic energy source (McIlroy et al., anaerobic uptake and storage of volatile fatty acids (VFAs) 2014). Most notably, the Tetrasphaera spp. and Microlunatus as polyhydroxyalkanoates (PHAs). Hydrolysis of aerobically phosphovorusT, both within the phylum Actinobacteria, cycle stored glycogen, and activity of the tricarboxylic acid (TCA) polyphosphate without PHA storage with dynamic feast–famine cycle provides required reducing power and additional conditions. Instead, these organisms exhibit a fermentative energy. Stored PHAs are utilized under subsequent aerobic metabolism where polyphosphate supplements anaerobic conditions supporting growth and replenishing glycogen and energy demands (Nakamura et al., 1995; Kong et al., 2005; polyphosphate stores, with wastage of aerobic biomass giving Kristiansen et al., 2013). Importantly, surveys of full-scale net P removal (Comeau et al., 1986; Wentzel et al., 1986: systems revealed that the Tetrasphaera spp. are in much higher Mino et al., 1987). Such a phenotype has been demonstrated abundance than the “Ca. Accumulibacter” PAO, questioning in the widely studied “Ca. Accumulibacter” genus within the the long-held belief that the latter classical-PAO are the most Betaproteobacteria (Hesselmann et al., 1999; He and McMahon, important for EBPR (Nguyen et al., 2011; Mielczarek et al., 2011). 2013a; Saunders et al., 2016) and reinforcing the need to Although EBPR systems are considered as an economical consider diverse physiologies for organisms important for strategy for wastewater treatment, they are subject to periods EBPR. of inefficiency and failure. Deterioration of EBPR has been In addition to the fermentative actinobacterial PAO, it has attributed to a variety of conditions; such as high rainfall, been shown that in dynamic feast–famine systems unidentified nutrient limitation, and high nitrate loading to the anaerobic organisms can store glucose directly as glycogen anaerobically, zone (Oehmen et al., 2007). Another proposed reason is energized by fermentation, without cycling polyphosphate microbial competition, where the proliferation of organisms (Carucci et al., 1999). The role of these “fermentative GAO” competing for anaerobic carbon supply, without excess in EBPR is of interest, given their potential as competitors of polyphosphate storage, is at the theoretical expense of P removal the abundant fermentative Tetrasphaera PAO. A likely candidate efficiency (Satoh et al., 1994). Bacteria with the glycogen for this phenotype is the activated sludge isolate Micropruina accumulating organism (GAO) phenotype have received glycogenicaT – a member of the family Propionibacteriaceae considerable attention as potential
Load more

Recommended publications
  • Bacterial Community Structure in Waste Water Treatment
    International Journal of Research Studies in Microbiology and Biotechnology (IJRSMB) Volume 3, Issue 1, 2017, PP 1-9 ISSN 2454-9428 (Online) http://dx.doi.org/10.20431/2454-9428.0301001 www.arcjournals.org Bacterial Community Structure in Waste Water Treatment Hiral Borasiya & Shah MP Division of Applied & Environmental Microbiology, Enviro Technology Limited, Industrial Waste Water Research Laboratory, Gujarat, India [email protected] Abstract: All data suggest that microbial community structures or samples of sludge with a content of phosphate between 8 and 12% were very similar but distinct from those containing phosphate at 1.8%. In all samples analyzed, ubiquinones, menaquinone and fatty acids were the main components. Dominance and E5 suggested that a large number of organisms belonging to the b and subclasses Proteobacteria and Actinobacteria from higher GMC Gram-positive bacteria, respectively, were present. Denaturing gradient gel electrophoresis analysis revealed at least 6-10 predominant DNA bands and numerous other fragments in each sample. Five major denaturing gradient gel electrophoresis fragments from each of 1.8% and 11.8% phosphate containing sludge samples, respectively, were successfully isolated and sequenced. Phylogenetic analysis of the sequences revealed that both 3% and 15% phosphate -containing sludge samples shared three common phylotypes which are separately associated with new bacterial groups of subclass C Proteobacteria, two E5 containing Actinobacteria, and Caulobacter spp. The subclass Proteobacteria. Phylogenetic analysis revealed useful phylotypes unique for both samples sludge. Therefore, changes in the phosphate content did not affect the composition and quantity prevailing microbial population, although specific phylotypes could not be unambiguously associated with EBPR.
    [Show full text]
  • Midas 4: a Global Catalogue of Full-Length 16S Rrna Gene
    bioRxiv preprint doi: https://doi.org/10.1101/2021.07.06.451231; this version posted July 6, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. MiDAS ‌ 4: ‌ A ‌ global ‌ catalogue‌ of ‌ full-length ‌ 16S ‌ rRNA‌ gene ‌ sequences ‌ and‌ ‌ taxonomy for‌ studies ‌ of‌ bacterial‌ communities‌ in‌ wastewater‌ treatment‌ plants‌ Authors: Morten Simonsen Dueholm, Marta Nierychlo, Kasper Skytte Andersen, Vibeke ‌ Rudkjøbing, Simon Knutsson, the MiDAS Global Consortium, Mads Albertsen, and Per ‌ Halkjær Nielsen* Affiliation: Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark. *Correspondence to: Per Halkjær Nielsen, Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, ‌ 9220 Aalborg, Denmark; Phone: +45 9940 8503; Fax: Not available; E-mail: [email protected] Running title: Global microbiota of wastewater treatment plants ‌ 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.07.06.451231; this version posted July 6, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Abstract Biological wastewater treatment and an increased focus on resource recovery is ‌ fundamental for environmental protection, human health, and sustainable development. Microbial communities are responsible for these processes, but our knowledge of their diversity and function is still poor, partly due to the ‌lack of ‌good reference databases and comprehensive global studies.
    [Show full text]
  • Table S5. the Information of the Bacteria Annotated in the Soil Community at Species Level
    Table S5. The information of the bacteria annotated in the soil community at species level No. Phylum Class Order Family Genus Species The number of contigs Abundance(%) 1 Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus 1749 5.145782459 2 Bacteroidetes Cytophagia Cytophagales Hymenobacteraceae Hymenobacter Hymenobacter sedentarius 1538 4.52499338 3 Gemmatimonadetes Gemmatimonadetes Gemmatimonadales Gemmatimonadaceae Gemmatirosa Gemmatirosa kalamazoonesis 1020 3.000970902 4 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas indica 797 2.344876284 5 Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus Lactococcus piscium 542 1.594633558 6 Actinobacteria Thermoleophilia Solirubrobacterales Conexibacteraceae Conexibacter Conexibacter woesei 471 1.385742446 7 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas taxi 430 1.265115184 8 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas wittichii 388 1.141545794 9 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas sp. FARSPH 298 0.876754244 10 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sorangium cellulosum 260 0.764953367 11 Proteobacteria Deltaproteobacteria Myxococcales Polyangiaceae Sorangium Sphingomonas sp. Cra20 260 0.764953367 12 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas panacis 252 0.741416341
    [Show full text]
  • Propionicicella Superfundia Gen. Nov., Sp. Nov., a Chlorosolvent-Tolerant Propionate-Forming, Facultative Anaerobic Bacterium Is
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Estudo Geral ARTICLE IN PRESS Systematic and Applied Microbiology 29 (2006) 404–413 www.elsevier.de/syapm Propionicicella superfundia gen. nov., sp. nov., a chlorosolvent-tolerant propionate-forming, facultative anaerobic bacterium isolated from contaminated groundwater Hee-Sung Baea, William M. Moeb,Ã, Jun Yanb, Igor Tiagoc, Milton S. da Costad, Fred A. Raineya aDepartment of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA bDepartment of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA cDepartamento de Zoologia and Centro de Neurocieˆncias, Universidade de Coimbra, 3004-517 Coimbra, Portugal dDepartamento de Bioquı´mica and Centro de Neurocieˆncias, Universidade de Coimbra, 3001-401 Coimbra, Portugal Received 5 October 2005 Abstract A novel strain, designated as BL-10T, was characterized using a polyphasic approach after isolation from groundwater contaminated by a mixture of chlorosolvents that included 1,1,2-trichloroethane, 1,2-dichloroethane, and vinyl chloride. Stain BL-10T is a facultatively anaerobic bacterium able to ferment glucose to form propionate, acetate, formate, lactate, and succinate. Fermentation occurred in the presence of 1,2-dichloroethane and 1,1,2-trichloroethane at concentrations to at least 9.8 and 5.9 mM, respectively. Cells are Gram-positive, rod-shaped, non-motile, and do not form spores. Oxidase and catalase are not produced and nitrate reduction did not occur in PYG medium. Menaquinone MK-9 is the predominant respiratory quinone and meso-diaminopimelic acid is present in the cell wall peptidoglycan layer. Major cellular fatty acids are C15:0, iso C16:0, and anteiso C15:0.
    [Show full text]
  • Mitigating Biofouling on Reverse Osmosis Membranes Via Greener Preservatives
    Mitigating biofouling on reverse osmosis membranes via greener preservatives by Anna Curtin Biology (BSc), Le Moyne College, 2017 A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of MASTER OF APPLIED SCIENCE in the Department of Civil Engineering, University of Victoria © Anna Curtin, 2020 University of Victoria All rights reserved. This Thesis may not be reproduced in whole or in part, by photocopy or other means, without the permission of the author. Supervisory Committee Mitigating biofouling on reverse osmosis membranes via greener preservatives by Anna Curtin Biology (BSc), Le Moyne College, 2017 Supervisory Committee Heather Buckley, Department of Civil Engineering Supervisor Caetano Dorea, Department of Civil Engineering, Civil Engineering Departmental Member ii Abstract Water scarcity is an issue faced across the globe that is only expected to worsen in the coming years. We are therefore in need of methods for treating non-traditional sources of water. One promising method is desalination of brackish and seawater via reverse osmosis (RO). RO, however, is limited by biofouling, which is the buildup of organisms at the water-membrane interface. Biofouling causes the RO membrane to clog over time, which increases the energy requirement of the system. Eventually, the RO membrane must be treated, which tends to damage the membrane, reducing its lifespan. Additionally, antifoulant chemicals have the potential to create antimicrobial resistance, especially if they remain undegraded in the concentrate water. Finally, the hazard of chemicals used to treat biofouling must be acknowledged because although unlikely, smaller molecules run the risk of passing through the membrane and negatively impacting humans and the environment.
    [Show full text]
  • Comparison of Methods to Identify Pathogens and Associated Virulence Functional Genes in Biosolids from Two Different Wastewater Treatment Facilities in Canada
    RESEARCH ARTICLE Comparison of Methods to Identify Pathogens and Associated Virulence Functional Genes in Biosolids from Two Different Wastewater Treatment Facilities in Canada Etienne Yergeau1*, Luke Masson2, Miria Elias1, Shurong Xiang3, Ewa Madey4, a11111 Hongsheng Huang5, Brian Brooks5, Lee A. Beaudette3 1 National Research Council Canada, Energy Mining and Environment, Montreal, Qc, Canada, 2 National Research Council Canada, Human Health Therapeutics, Montreal, Qc, Canada, 3 Environment Canada, Biological Assessment and Standardization Section, Ottawa, On, Canada, 4 Canadian Food Inspection Agency, Fertilizer Safety Office, Plant Health & Biosecurity Directorate, Ottawa, On, Canada, 5 Canadian Food Inspection Agency, Ottawa Laboratory – Fallowfield, Ottawa, On, Canada OPEN ACCESS * [email protected] Citation: Yergeau E, Masson L, Elias M, Xiang S, Madey E, Huang H, et al. (2016) Comparison of Methods to Identify Pathogens and Associated Abstract Virulence Functional Genes in Biosolids from Two Different Wastewater Treatment Facilities in Canada. The use of treated municipal wastewater residues (biosolids) as fertilizers is an attractive, PLoS ONE 11(4): e0153554. doi:10.1371/journal. inexpensive option for growers and farmers. Various regulatory bodies typically employ pone.0153554 indicator organisms (fecal coliforms, E. coli and Salmonella) to assess the adequacy and Editor: Leonard Simon van Overbeek, Wageningen efficiency of the wastewater treatment process in reducing pathogen loads in the final University and Research Centre, NETHERLANDS product. Molecular detection approaches can offer some advantages over culture-based Received: October 28, 2015 methods as they can simultaneously detect a wider microbial species range, including Accepted: March 31, 2016 non-cultivable microorganisms. However, they cannot directly assess the viability of the Published: April 18, 2016 pathogens.
    [Show full text]
  • Systematic Research on Actinomycetes Selected According
    Systematic Research on Actinomycetes Selected according to Biological Activities Dissertation Submitted in fulfillment of the requirements for the award of the Doctor (Ph.D.) degree of the Math.-Nat. Fakultät of the Christian-Albrechts-Universität in Kiel By MSci. - Biol. Yi Jiang Leibniz-Institut für Meereswissenschaften, IFM-GEOMAR, Marine Mikrobiologie, Düsternbrooker Weg 20, D-24105 Kiel, Germany Supervised by Prof. Dr. Johannes F. Imhoff Kiel 2009 Referent: Prof. Dr. Johannes F. Imhoff Korreferent: ______________________ Tag der mündlichen Prüfung: Kiel, ____________ Zum Druck genehmigt: Kiel, _____________ Summary Content Chapter 1 Introduction 1 Chapter 2 Habitats, Isolation and Identification 24 Chapter 3 Streptomyces hainanensis sp. nov., a new member of the genus Streptomyces 38 Chapter 4 Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae 52 Chapter 5 A new member of the family Micromonosporaceae, Planosporangium flavogriseum gen nov., sp. nov. 67 Chapter 6 Promicromonospora flava sp. nov., isolated from sediment of the Baltic Sea 87 Chapter 7 Discussion 99 Appendix a Resume, Publication list and Patent 115 Appendix b Medium list 122 Appendix c Abbreviations 126 Appendix d Poster (2007 VAAM, Germany) 127 Appendix e List of research strains 128 Acknowledgements 134 Erklärung 136 Summary Actinomycetes (Actinobacteria) are the group of bacteria producing most of the bioactive metabolites. Approx. 100 out of 150 antibiotics used in human therapy and agriculture are produced by actinomycetes. Finding novel leader compounds from actinomycetes is still one of the promising approaches to develop new pharmaceuticals. The aim of this study was to find new species and genera of actinomycetes as the basis for the discovery of new leader compounds for pharmaceuticals.
    [Show full text]
  • Of Bergey's Manual
    BERGEY’S MANUAL® OF Systematic Bacteriology Second Edition Volume Five The Actinobacteria, Part A and B BERGEY’S MANUAL® OF Systematic Bacteriology Second Edition Volume Five The Actinobacteria, Part A and B Michael Goodfellow, Peter Kämpfer, Hans-Jürgen Busse, Martha E. Trujillo, Ken-ichiro Suzuki, Wolfgang Ludwig and William B. Whitman EDITORS, VOLUME FIVE William B. Whitman DIRECTOR OF THE EDITORIAL OFFICE Aidan C. Parte MANAGING EDITOR EDITORIAL BOARD Fred A. Rainey, Chairman, Peter Kämpfer, Vice Chairman, Paul De Vos, Jongsik Chun, Martha E. Trujillo and William B. Whitman WITH CONTRIBUTIONS FROM 116 COLLEAGUES William B. Whitman Bergey’s Manual Trust Department of Microbiology 527 Biological Sciences Building University of Georgia Athens, GA 30602-2605 USA ISBN 978-0-387-95043-3 ISBN 978-0-387-68233-4 (eBook) DOI 10.1007/978-0-387-68233-4 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2012930836 © 2012, 1984–1989 Bergey’s Manual Trust Bergey’s Manual is a registered trademark of Bergey’s Manual Trust. All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights.
    [Show full text]
  • Diversity and Function of the Microbial Community on Anodes of Sediment Microbial Fuel Cells Fueled by Root Exudates
    Diversity and Function of the Microbial Community on Anodes of Sediment Microbial Fuel Cells fueled by Root Exudates Doctoral thesis Submitted in partial fulfillment of the requirement for a doctoral degree “Doktorgrad der Naturwissenschaften (Dr. rer. nat.)” to the faculty of biology – Philipps-Universität Marburg by Angela Cabezas da Rosa from Montevideo, Uruguay Marburg / Lahn | 2010 The research for the completion of this work was carried out from April 2007 to September 2010 at the Max-Planck Institute for Terrestrial Microbiology under the supervision of Prof. Michael W. Friedrich Thesis was submitted to the Faculty of Biology, Philipps-Universität, Marburg Doctoral thesis accepted on: 24.11.2010 Date of oral examination: 26.11.2010 First reviewer: Prof. Dr. Michael W. Friedrich Second reviewer: Prof. Dr. Wolfgang Buckel The following manuscripts originated from this work and were published or are in preparation: De Schamphelaire L, Cabezas A, Marzorati M, Friedrich MW, Boon N & Verstraete W (2010) Microbial Community Analysis of Anodes from Sediment Microbial Fuel Cells Powered by Rhizodeposits of Living Rice Plants. Applied and Environmental Microbiology 76: 2002-2008. Cabezas A, de Schamphelaire L, Boon N, Verstraete W, Friedrich MW. Rice root exudates select for novel electrogenic Geobacter and Anaeromyxobacter populations on sediment microbial fuel cell anodes. In preparation. Cabezas A, Köhler T, Brune A, Friedrich MW. Identification of β-Proteobacteria and Anaerolineae as active populations degrading rice root exudates on
    [Show full text]
  • Transfer of Skin Microbiota Between Two Dissimilar Autologous Microenvironments: a Pilot Study
    RESEARCH ARTICLE Transfer of skin microbiota between two dissimilar autologous microenvironments: A pilot study 1 2¤ 2,3 Benji PerinID *, Amin Addetia , Xuan Qin 1 University of Washington Division of Dermatology and Dermatology Residency, Seattle, WA, United States of America, 2 Seattle Children's Hospital, Seattle, WA, United States of America, 3 University of Washington Department of Laboratory Medicine, Seattle, WA, United States of America a1111111111 ¤ Current address: University of Washington Department of Laboratory Medicine, Seattle, WA, United States a1111111111 of America a1111111111 * [email protected] a1111111111 a1111111111 Abstract Dysbiosis of skin microbiota is associated with several inflammatory skin conditions, includ- ing atopic dermatitis, acne, and hidradenitis suppurativa. There is a surge of interest by clini- OPEN ACCESS cians and the lay public to explore targeted bacteriotherapy to treat these dermatologic Citation: Perin B, Addetia A, Qin X (2019) Transfer conditions. To date, skin microbiota transplantation studies have focused on moving single, of skin microbiota between two dissimilar autologous microenvironments: A pilot study. enriched strains of bacteria to target sites rather than a whole community. In this prospective PLoS ONE 14(12): e0226857. https://doi.org/ pilot study, we examined the feasibility of transferring unenriched skin microbiota communi- 10.1371/journal.pone.0226857 ties between two anatomical sites of the same host. We enrolled four healthy volunteers Editor: Miroslav Blumenberg, NYU Langone (median age: 28 [range: 24, 36] years; 2 [50%] female) who underwent collection and trans- Medical Center, UNITED STATES fer of skin microbiota from the forearm to the back unidirectionally. Using culture methods Received: August 25, 2019 and 16S rRNA V1-V3 deep sequencing, we compared baseline and mixed ("transplant") Accepted: December 5, 2019 communities, at T = 0 and T = 24 hours.
    [Show full text]
  • Propionicicella Superfundia Gen. Nov., Sp. Nov., a Chlorosolvent-Tolerant Propionate-Forming, Facultative Anaerobic Bacterium Is
    ARTICLE IN PRESS Systematic and Applied Microbiology 29 (2006) 404–413 www.elsevier.de/syapm Propionicicella superfundia gen. nov., sp. nov., a chlorosolvent-tolerant propionate-forming, facultative anaerobic bacterium isolated from contaminated groundwater Hee-Sung Baea, William M. Moeb,Ã, Jun Yanb, Igor Tiagoc, Milton S. da Costad, Fred A. Raineya aDepartment of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA bDepartment of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA cDepartamento de Zoologia and Centro de Neurocieˆncias, Universidade de Coimbra, 3004-517 Coimbra, Portugal dDepartamento de Bioquı´mica and Centro de Neurocieˆncias, Universidade de Coimbra, 3001-401 Coimbra, Portugal Received 5 October 2005 Abstract A novel strain, designated as BL-10T, was characterized using a polyphasic approach after isolation from groundwater contaminated by a mixture of chlorosolvents that included 1,1,2-trichloroethane, 1,2-dichloroethane, and vinyl chloride. Stain BL-10T is a facultatively anaerobic bacterium able to ferment glucose to form propionate, acetate, formate, lactate, and succinate. Fermentation occurred in the presence of 1,2-dichloroethane and 1,1,2-trichloroethane at concentrations to at least 9.8 and 5.9 mM, respectively. Cells are Gram-positive, rod-shaped, non-motile, and do not form spores. Oxidase and catalase are not produced and nitrate reduction did not occur in PYG medium. Menaquinone MK-9 is the predominant respiratory quinone and meso-diaminopimelic acid is present in the cell wall peptidoglycan layer. Major cellular fatty acids are C15:0, iso C16:0, and anteiso C15:0. Genomic DNA G+C content is 69.9 mol%.
    [Show full text]
  • Enhanced Biological Phosphorus Removal from Wastewater in the Tropics
    This document is downloaded from DR‑NTU (https://dr.ntu.edu.sg) Nanyang Technological University, Singapore. Enhanced biological phosphorus removal from wastewater in the tropics Cokro, Angel Anisa 2018 Cokro, A. A. (2018). Enhanced biological phosphorus removal from wastewater in the tropics. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/75924 https://doi.org/10.32657/10356/75924 Downloaded on 24 Sep 2021 05:49:19 SGT Enhanced Biological Phosphorus Removal from Wastewater in the Tropics Angel Anisa Cokro School of Civil and Environmental Engineering 2018 Enhanced Biological Phosphorus Removal from Wastewater in the Tropics Angel Anisa Cokro School of Civil and Environmental Engineering Nanyang Technological University A thesis submitted to Nanyang Technological University in partial fulfilment of the requirement for the degree of Doctor of Philosophy 2018 ACKNOWLEDGEMENTS I would like to take this opportunity to express my sincerest gratitude to those who have supported me during my journey as a PhD student. First and foremost, I would like to thank Prof. Stefan Wuertz for his continuous support and guidance. Thank you for giving me the chance to embark on this exciting PhD programme in the first place. Thank you for your constant encouragement so that I can continuously grow as a scientist. It is a great privilege for me to complete my PhD under your guidance, and I am extremely thankful for all the valuable lessons that I have learned. I am grateful for the research grant, which is supported by the Singapore National Research Foundation under its Environment & Water Research Programme and administered by PUB, Singapore’s national water agency, and the Ministry of Education under the Research Centre of Excellence Programme.
    [Show full text]