Yalkut-4883.Pdf
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
LC-MS and LC-PDA Analysis of Hypericum Empetrifolium and Hypericum Sinaicum Feras Q
LC-MS and LC-PDA Analysis of Hypericum empetrifolium and Hypericum sinaicum Feras Q. Alalia,*, Khaled Tawahab, and Mohammad Gharaibehc a Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Al Isra Private University, P. O. Box 22, 33, Amman 11622, Jordan. E-mail: [email protected] b Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman 11942, Jordan c Department of Natural Resources and Environment, Faculty of Agriculture, Jordan University of Science and Technology, P. O. Box 3030, Irbid 22110, Jordan * Author for correspondence and reprint requests Z. Naturforsch. 64 c, 476 – 482 (2009); received February 25/March 26, 2009 Within the framework of our continuous efforts to explore Hypericum species from Jor- dan, we report the analysis of the major active metabolites, naphthodianthrones and phloro- glucinols, in the methanolic extracts of two under-explored Hypericum species; H. empetri- folium Willd. and H. sinaicum Hochst. & Steud. ex Boiss., using LC-(+,–)-ESI-MS (TIC and SIM) and LC-UV/Vis spectroscopy. Based on their LC-UV/Vis profi les, retention times and (+,–)-ESI-MS (TIC and SIM) spectral data, hypericin, protohypericin and pseudohypericin were identifi ed in both of the investigated species. In addition adhyperfi rin was only de- tected in H. empetrifolium, while hyperforin and protopseudohypericin were only detected in H. sinaicum. This is the fi rst report documenting the presence of hypericin, protohypericin, pseudohypericin, protopseudohypericin, and hyperforin in H. sinaicum, and adhyperfi rin in H. empetrifolium. Key words: Hypericum, Naphthodianthrones, Phloroglucinols Introduction species of Hypericum can be identifi ed by: (i) op- posite simple entire exstipulate leaves contain- Hypericum is a genus of about 450 species ing translucent and often black or red glandular of herbs or shrubs belonging to the family Clu- secretions; (ii) fl owers with a 5-merous perianth siaceae, formerly Hypericaceae. -
Die Soinmerflora Des Antilibanon
© Zool.-Bot. Ges. Österreich, Austria; download unter www.biologiezentrum.at Die Soinmerflora des Antilibanon. Yon Dr. Theodor Kotschy. Vorgetragen in der Sitzung vom 6- November 1861. -L'as Land Syrien, von Nord nach Süd langgestreckt, im West vom Mittel- meer, im Ost von der einem Sandmeere gleichenden Wüste begrenzt, bildet ein natürliches Verbindungsglied zwischen dem Hochlande des Tauriisgebietes und dem Tieflande Egypten's. Drei Gebirgketten sind es, welche ihm wesentlich seine plastische Gestaltung geben: der Amanus, Libanon und Antilibanon. Ihre Berg- züge, Hochrücken, Gipfelreihen und Thalspalten folgen vorzugsweise der Längenachse des Landes. Der Jordan und Orontes sind Gegenströme und ihre Normalrichtung bezeichnet den Parallelismus der Hauptthäler Syrien's. Dieses Land, dessen folgenreiche Geschichte einen so entschiedenen Einfluss auf die ganze übrige Welt genommen hat, zeigt im Osten beider Hauptströme ein Binnenland mit einer Plateaufläche bei 1200—2000 Fuss mittlerer Höhe über dem Meer, von weit gegen Osten hin gleichförmiger Ausdehnung. Die Westgrenzen bildet ein Küstensaum, niedrig gelegen und mehr oder minder breit.' Zwischen beiden liegt als Mittelglied der aus den drei genannten Gruppen bestehende Gebirgszug, wechselnd an mannigfaltiger Gestaltung und. Erhebung bis zu der Höhe von 9500 Fuss. Durch die Verschiedenheit der geognostischen Unterlage unterscheiden sich diese drei Bergsysteme in ihrem Baue von einander. Der Amanus weicht-von den beiden südlichen Ketten, welche vor- herrschend aus Kalkstein bestehen, gänzlich ab, indem bei seiner Entste- hung grösstentheils plutonische Eruptionen thätig waren. Die Höhen sind theilweise abgerundet, theilweise mehr ausgezeichnet kantig, ja im nörd- lichen Theil kegelförmig und steigen sogar im Duldul nadelartig auf. Der südliche Theil hat einen trachytischen Charakter aus Aphauit, Serpentin, Euphotid im Hauptzug , der übrige Theil wechselt mit crystallinischen und massigen Gesteinen und den Gebilden aus der Kreidereihe ab. -
Genetic Variation in Sinai's Range-Restricted Plant Taxa <I>Hypericum Sinaicum</I> and <I>Origanum Syriacu
Plant Ecology and Evolution 147 (2): 187–201, 2014 http://dx.doi.org/10.5091/plecevo.2014.838 REGULAR PAPER Genetic variation in Sinai’s range-restricted plant taxa Hypericum sinaicum and Origanum syriacum subsp. sinaicum and its conservational implications Mohamed S. Zaghloul1,*, Peter Poschlod2 & Christoph Reisch2 1Botany Department, Suez Canal University, Ismailia, Egypt 2Institut für Botanik, Universität Regensburg, DE-93053 Regensburg, Germany *Author for correspondence: [email protected] Background and aims – It is a key conservation aim to maintain genetic diversity within populations of rare and threatened species. The flora of the Sinai Peninsula is unique and, therefore, of strong interest. However, in only few studies genetic structure and variation within and among populations of Sinai plants have been analysed. In the study presented here, we analysed the genetic structure of Hypericum sinaicum and Origanum syriacum subsp. sinaicum, which are two rare respectively near-endemic and endemic medicinal perennial plants with overlapping ranges restricted to the mountainous region of southern Sinai in Egypt. Methods and key results – We used AFLP markers and calculated standard genetic diversity measures. Both species exhibited much higher genetic diversity and lower genetic differentiation than generally reported for endemic plants. Although the taxa differed in distribution range and density of populations in the study region (local scale), molecular variation within populations was not significantly different between both taxa. H. sinaicum, the taxon with the narrower range and fewer populations, exhibited a stronger population differentiation than O. syriacum subsp. sinaicum, the taxon with the wider range and more populations at the scale of the study. Populations of both species followed the isolation-by-distance model. -
Checklist of the Vascular Plants of San Diego County 5Th Edition
cHeckliSt of tHe vaScUlaR PlaNtS of SaN DieGo coUNty 5th edition Pinus torreyana subsp. torreyana Downingia concolor var. brevior Thermopsis californica var. semota Pogogyne abramsii Hulsea californica Cylindropuntia fosbergii Dudleya brevifolia Chorizanthe orcuttiana Astragalus deanei by Jon P. Rebman and Michael G. Simpson San Diego Natural History Museum and San Diego State University examples of checklist taxa: SPecieS SPecieS iNfRaSPecieS iNfRaSPecieS NaMe aUtHoR RaNk & NaMe aUtHoR Eriodictyon trichocalyx A. Heller var. lanatum (Brand) Jepson {SD 135251} [E. t. subsp. l. (Brand) Munz] Hairy yerba Santa SyNoNyM SyMBol foR NoN-NATIVE, NATURaliZeD PlaNt *Erodium cicutarium (L.) Aiton {SD 122398} red-Stem Filaree/StorkSbill HeRBaRiUM SPeciMeN coMMoN DocUMeNTATION NaMe SyMBol foR PlaNt Not liSteD iN THE JEPSON MANUAL †Rhus aromatica Aiton var. simplicifolia (Greene) Conquist {SD 118139} Single-leaF SkunkbruSH SyMBol foR StRict eNDeMic TO SaN DieGo coUNty §§Dudleya brevifolia (Moran) Moran {SD 130030} SHort-leaF dudleya [D. blochmaniae (Eastw.) Moran subsp. brevifolia Moran] 1B.1 S1.1 G2t1 ce SyMBol foR NeaR eNDeMic TO SaN DieGo coUNty §Nolina interrata Gentry {SD 79876} deHeSa nolina 1B.1 S2 G2 ce eNviRoNMeNTAL liStiNG SyMBol foR MiSiDeNtifieD PlaNt, Not occURRiNG iN coUNty (Note: this symbol used in appendix 1 only.) ?Cirsium brevistylum Cronq. indian tHiStle i checklist of the vascular plants of san Diego county 5th edition by Jon p. rebman and Michael g. simpson san Diego natural history Museum and san Diego state university publication of: san Diego natural history Museum san Diego, california ii Copyright © 2014 by Jon P. Rebman and Michael G. Simpson Fifth edition 2014. isBn 0-918969-08-5 Copyright © 2006 by Jon P. -
Agrobacterium Rhizogenes-Mediated Transformation of Hypericum Sinaicum L
Brazilian Journal of Pharmaceutical Sciences Article http://dx.doi.org/10.1590/s2175-97902020000118327 Agrobacterium rhizogenes-mediated transformation of Hypericum sinaicum L. for the development of hairy roots containing hypericin Heba Desouky Khlifa1*, Magdalena Klimek-Chodacka2, Rafal Baranski2, Michal Combik3, Hussein Sayed Taha iD 1* 1Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Dokki, Cairo, Egypt, 2Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Krakow, Poland, 3Vascular Plants Department, W. Szafer Institute of Botany, Polish Academy of Sciences, Krakow, Poland Hypericum sinaicum L. is an endangered Egyptian medicinal plant of high importance due to the presence of naphthodianthrones (hypericins), which have photodynamic properties and pharmaceutical potential. We sought to assess H. sinaicum ability to develop hairy roots that could be cultured in contained conditions in vitro and used as a source for hypericin production. We used four A. rhizogenes strains differing in their plasmids and chromosomal backgrounds to inoculate excised H. sinaicum root, stem and leaf explants to induce hairy root development. Additionally, inoculum was applied to shoots held in Rockwool cubes supporting their stand after removal of the root system. All explant types were susceptible to A. rhizogenes although stem explants responded more frequently (over 90%) than other explant types. The A4 and A4T A. rhizogenes strains were highly, and equally effective in hairy root induction on 66-72% of explants while the LBA1334 strain was the most effective in transformation of shoots. Sonication applied to explants during inoculation enhanced the frequency of hairy root development, the most effective was 60 s treatment doubling the percentage of explants with hairy roots. -
²êî²æ²üæ² Takhtajania Тахтаджяния
вÚÎ²Î²Ü ´àôê²´²Ü²Î²Ü ÀÜκðàôÂÚàôÜ Ð²Ú²êî²ÜÆ Ð²Üð²äºîàôÂÚ²Ü ¶ÆîàôÂÚàôÜÜºðÆ ²¼¶²ÚÆÜ ²Î²¸ºØÆ²ÚÆ ²© ²Êî²æÚ²ÜÆ ²Üì²Ü ´àôê²´²ÜàôÂÚ²Ü ÆÜêîÆîàôî ARMENIAN BOTANICAL SOCIETY INSTITUTE OF BOTANY AFTER A. TAKHTAJYAN OF NATIONAL ACADEMY OF SCIENCES REPUBLIC ARMENIA АРМЯНСКОЕ БОТАНИЧЕСКОЕ ОБЩЕСТВО ИНСТИТУТ БОТАНИКИ ИМ. А. ТАХТАДЖЯНА НАЦИОНАЛЬНОЙ АКАДЕМИИ НАУК РЕСПУБЛИКИ АРМЕНИЯ Â²Êî²æ²ÜƲ äñ³Ï 4 TAKHTAJANIA Issue 4 ТАХТАДЖЯНИЯ Выпуск 4 ºñ¨³Ý Yerevan Ереван 2018 УДК 581. 9 ББК 28.5 Т244 Печатается по решению редакционного совета TAKHTAJANIA Редакционный совет: Варданян Ж. А., Грёйтер В. (Палермо), Аверьянов Л. В. (Санкт-Петербург), Гельтман Д. В. (Санкт-Петербург), Витек Э. (Вена), Осипян Л. Л., Нанагюлян С. Г. Редакционная коллегия: Оганезова Г. Г. (главный редактор), Оганесян М. Э., Файвуш Г. М., Элбакян А. А. (ответственный секретарь) Takhtajania /Армянское ботаническое общ-во, Институт ботаники им. А. Тахтаджяна НАН РА; Т 244 Ред. коллегия: Оганезова Г. Г. и др. – Ер.: Арм. ботаническое общество, 2018. Вып. 4. – 132 с. Основной тематикой сборника являются систематика растений, морфология, анатомия, флористика, эволюция, палинология, кариология, палеоботаника, геоботаника, биология и другие проблемы. 0040, Армения, Ереван, ул. Ачаряна 1, Армянское ботаническое общество (редакция Takhtajania). Телефон: (37410) 61 42 41; e-mail: [email protected] ВАК Армении включает Тахтаджания в перечень периодических научных изданий, в которых могут быть опубликованы основные результаты и положения для кандидатских диссертаций Рецензируемое издание Следующие выпуски Тахтаджания будут выходить ежегодно только в электронном виде Электронный вариант доступен на сайте www.flib.sci.am ISBN 978 –99941–2–564–7 УДК 581. 9 ББК 28.5 © Арм. -
Hypericum Sinaicum L. in Vitro Regeneration and Analysis of Hypericin Content
Int.J.Curr.Microbiol.App.Sci (2016) 5(8): 182-196 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 8 (2016) pp. 182-196 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.508.020 Hypericum sinaicum L. in Vitro Regeneration and Analysis of Hypericin Content Heba D. Khlifa1, Ibrahim A. Ibrahim2, M. Bekhit2, M. Szkop3 and H.S. Taha1* 1Plant Biotechnology Department, National Research Centre (NRC), Dokki, P.O.12622Cairo Egypt 2Plant Biotechnology Dept., Genetic Engineering Institute (GEBRI), University of Sadat City, Egypt 3Biochemistry Department, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (SGGW), Poland *Corresponding author ABSTRACT The present study aimed to use plant biotechnology techniques and biochemical analyses to achieve promising procedures for regenerated shootlets of Hypericum sinaicum L. via direct/ indirect regeneration. So and related to this concern, this investigation was carried to examine the effect of different explant types and culture conditions, with emphasis on the type and concentrations of plant growth K e yw or ds regulators (PGRs) in culture media on shootlets regeneration and prolonged Hypericum maintenance of in vitro material. The results showed that, direct/indirect shoot sinaicum , formation were induced by all levels of PGRs but the optimum medium for direct in vitro shootlets induction and multiplication was MS supplemented with 0.1 mg/l NAA propagation, combination with 1mg/l BA compared with other supplementations. The maximum regeneration, number of direct shootlets regeneration (24 shootlets per explant) was recorded mul tiplication, with nodal explant cultured on MS medium supplemented with 0.1 NAA mg/l plus hypericin. -
Phytochemische Und Pharmakologische in Vitro Untersuchungen Zu Hypericum Empetrifolium WILLD
Phytochemische und pharmakologische in vitro Untersuchungen zu Hypericum empetrifolium WILLD. Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften (Dr. rer. nat.) der Fakultät für Chemie und Pharmazie der Universität Regensburg vorgelegt von Apotheker Sebastian Schmidt aus Bamberg 2013 Diese Arbeit wurde im Zeitraum von September 2009 bis September 2013 unter der Anleitung von Herrn Prof. Dr. Jörg Heilmann am Lehrstuhl für Pharmazeutische Biologie der Universität Regensburg angefertigt. Das Promotionsgesuch wurde eingereicht am: 19. September 2013 Tag der mündlichen Prüfung: 08. November 2013 Prüfungsausschuss: Prof. Dr. Gerhard Franz (Vorsitzender) Prof. Dr. Jörg Heilmann (Erstgutachter) Prof. Dr. Adolf Nahrstedt (Zweitgutachter) Prof. Dr. Joachim Wegener (Dritter Prüfer) Danksagung Zuallererst möchte ich mich bei Prof. Dr. Jörg Heilmann für die Möglichkeit bedanken, in seinem Arbeitskreis zu arbeiten und eine Dissertation zu einem interessanten Thema anzufertigen. Vielen Dank, lieber Jörg, für Deine stets herzliche und freundschaftliche Art, mir in allen fachlichen Fragen, aber auch in privaten Dingen mit Rat und Tat zur Seite zu stehen. Meinem Freund Dr. Guido Jürgenliemk, der mir durch seine Erfahrung in phytochemischen, botanischen, zwischenmenschlichen und vor allem kulinarischen Problemen eine große Hilfe war, möchte ich ganz besonders danken. Lieber Guido, Du warst immer da! Dr. Birgit Kraus sei herzlich gedankt für Ihre Unterstützung im Umgang mit den Mikroskopen und den vielen guten Anregungen und Tipps rund um die Zellkultur. Bei Gabi Brunner und Anne Grashuber möchte ich mich and dieser Stelle ausdrücklich und von Herzen für die wertvolle Hilfe in allen praktischen Dingen bedanken. Unserer Sekretärin Hedwig Ohli wünsche ich Alles Gute und hoffe, dass sie ganz bald schon wieder die unterhaltsame erste Ansprechpartnerin im Sekretariat des Lehrstuhls sein wird. -
Asexual Propagation and Ex Situ Conservation of Hypericum
Journal of Medicinal Plants Studies 2018; 6(6): 235-241 ISSN (E): 2320-3862 ISSN (P): 2394-0530 Asexual propagation and ex situ conservation of NAAS Rating: 3.53 JMPS 2018; 6(6): 235-241 Hypericum empetrifolium Willd. Subsp. empetrifolium © 2018 JMPS Received: 05-09-2018 (Hypericaceae), an East Mediterranean medicinal Accepted: 06-10-2018 plant with ornamental value Virginia Sarropoulou Hellenic Agricultural, Organization (HAO)-Demeter, Virginia Sarropoulou, Nikos Krigas, Katerina Grigoriadou and Eleni Maloupa Institute of Plant Breeding and Genetic Resources, Laboratory Abstract of Protection and Evaluation of Native and Floriculture Species, Hypericum empetrifolium subsp. empetrifolium is a medicinal plant of conservation concern and Balkan Botanic Garden of ornamental value restricted to Greece, Albania, Western Turkey and Cyrenaica in Libya. For vegetative Kroussia, Thermi, Thessaloniki, propagation in mid-autumn, shoot tip softwood cuttings (5-6 cm) were immersed for 1 min in solutions Greece of 4 IBA concentrations (0, 1000, 2000 and 4000 mg L-1). Cuttings were placed in propagation trays in a peat: perlite (1:3) substrate, under mist. For in vitro propagation, shoot tips explants were cultured in MS Nikos Krigas medium. The effect of BA alone and combined with auxins on shoot proliferation was studied. For in Hellenic Agricultural, vitro rooting, different auxins (IBA, NAA, IAA) applied at different concentrations were tested. Highest Organization (HAO)-Demeter, rooting percentage for cuttings was noticed when using 1000 mg L-1 IBA (17.17 roots 2.84 cm long, Institute of Plant Breeding and 85.71% rooting) (6 weeks). The 0.1 mg L-1 BA + 0.01 mg L-1 IBA hormonal combination was the best for Genetic Resources, Laboratory in vitro shoot proliferation, yielding 5.5 shoots/explant, 100% shoot formation and 5.42 shoot of Protection and Evaluation of proliferation rate (4 weeks). -
Morphological and Phytochemical Diversity Among Hypericum Species of the Mediterranean Basin
® Medicinal and Aromatic Plant Science and Biotechnology ©2011 Global Science Books Morphological and Phytochemical Diversity among Hypericum Species of the Mediterranean Basin Nicolai M. Nürk1 • Sara L. Crockett2* 1 Leibniz Institute of Plant Genetics and Crop Research (IPK), Genbank – Taxonomy & Evolutionary Biology, Corrensstrasse 3, 06466 Gatersleben, Germany 2 Institute of Pharmaceutical Sciences, Department of Pharmacognosy, Universitätsplatz 4/1, Karl-Franzens-Universität Graz, 8010 Graz, Austria Corresponding author : * [email protected] ABSTRACT The genus Hypericum L. (St. John’s wort, Hypericaceae) includes more than 480 species that occur in temperature or tropical mountain regions of the world. Monographic work on the genus has resulted in the recognition and description of 36 taxonomic sections, delineated by specific combinations of morphological characteristics and biogeographic distribution. The Mediterranean Basin has been recognized as a hot spot of diversity for the genus Hypericum, and as such is a region in which many endemic species occur. Species belonging to sections distributed in this area of the world display considerable morphological and phytochemical diversity. Results of a cladistic analysis, based on 89 morphological characters that were considered phylogenetically informative, are given here. In addition, a brief overview of morphological characteristics and the distribution of pharmaceutically relevant secondary metabolites for species native to this region of the world are presented. _____________________________________________________________________________________________________________ -
Tàxons Informatitzats I Nombre De Plecs a BCN Tàxon N Plecs
Tàxons informatitzats i nombre de plecs a BCN Tàxon N plecs Aaronsohnia pubescens (Desf.) K.Bremer & Humphries 6 Abelia chinensis R.Br. 2 Abelia coronata 1 Abies alba Mill. 19 Abies cephalonica Loudon 1 Abies concolor (Gordon) Hildebr. 4 Abies grandis (D.Don) Lindl. 1 Abies pinsapo Boiss. 8 Abies pinsapo Boiss. subsp. marocana (Trab.) Emb. & Maire 1 Abies sp. 2 Abies x masjoanis 2 Abuta grandifolia (Mart.) Sandwith 1 Abutilon grandifolium (Willd.) Sweet 2 Abutilon pictum Walp. 2 Abutilon sp. 2 Abutilon striatum Dicks. ex Lindl. 4 Abutilon theophrasti Medik. 22 Acacia baileyana F.Muell. 3 Acacia calamifolia Lindl. 1 Acacia catechu (L.f.) Willd. 1 Acacia caven (Molina) Molina 1 Acacia cultriformis A.Cunn. 2 Acacia cyanophylla Lindl. 6 Acacia dealbata Link 15 Acacia decurrens (J.C.Wendl.) Willd. 1 Acacia eburnea (L.f.) Willd. 2 Acacia farnesiana (L.) Willd. 5 Acacia horrida Willd. 1 Acacia julibrissin Willd. 1 Acacia karoo Hayne 4 Acacia leiophylla Benth. 2 1 Tàxons informatitzats i nombre de plecs a BCN Acacia linifolia (Vent.) Willd. 1 Acacia longifolia (Andrews) Willd. 10 Acacia melanoxylon R.Br. 4 Acacia podalyriifolia A.Cunn. ex G.Don 2 Acacia polyphylla DC. 1 Acacia retinodes Schltdl. 12 Acacia rostellifera Benth. 2 Acacia salicina Lindl. 1 Acacia saligna (Labill.) H.L.Wendl. 3 Acacia seyal Delile 1 Acacia sp. 7 Acacia verticillata (L'Hér.) Willd. 1 Acacia wattsiana Benth. 1 Acalypha brachystachya Hornem. 1 Acalypha sp. 5 Acanthorrhinum ramosissimum (Coss. & Durieu) Rothm. 1 Acanthospermum australe (Loefl.) Kuntze 1 Acanthus mollis L. 7 Acanthus mollis L. subsp. platyphyllus Murb. 1 Acer campestre L. -
Anadolu Üniversitesi
HYPERICUM THYMOPSIS BOISS. (HYPERICACEAE) ÜZERİNDE FİTOKİMYASAL VE BİYOAKTİVİTE ÇALIŞMALARI Yüksek Lisans Tezi Benan OKYAY Eskişehir 2020 HYPERICUM THYMOPSIS BOISS. (HYPERICACEAE) ÜZERİNDE FİTOKİMYASAL VE BİYOAKTİVİTE ÇALIŞMALARI Benan OKYAY YÜKSEK LİSANS TEZİ Farmakognozi Anabilim Dalı Danışman: Prof. Dr. Nilgün ÖZTÜRK Eskişehir Anadolu Üniversitesi Sağlık Bilimleri Enstitüsü Temmuz 2020 Bu tez çalışması BAP Komisyonunca kabul edilen 1705S340 no.lu proje kapsamında desteklenmiştir. ÖZET HYPERICUM THYMOPSIS BOISS. (HYPERICACEAE) ÜZERİNDE FİTOKİMYASAL VE BİYOAKTİVİTE ÇALIŞMALARI Benan OKYAY Farmakognozi Anabilim Dalı Anadolu Üniversitesi Sağlık Bilimleri Enstitüsü, Temmuz 2020 Danışman: Prof. Dr. Nilgün ÖZTÜRK Bu çalışmada, Sivas-Kangal-Gürün yakınından toplanan endemik Hypericum thymopsis Boiss’in (Hypericaeae) toprak üstü kısımlarından hazırlanan farklı polaritedeki ekstrelerin ve infüzyonun toplam fenolik madde ve toplam flavonoid içerikleri spektrofotometrik metotla, hiperisin, psödohiperisin ve ayrıca fenolik asitlerinin miktarları ters-faz YBSK- DAD ile belirlenmiştir. Ayrıca, ekstrelerin antioksidan aktivitesi DPPH• serbest radikal süpürücü etki, ABTS katyon radikali süpürücü etki, metal şelatlama ve redükleyici güç tayinleri ile, asetilkolinesteraz ve butirilkolinesteraz enzim inhibisyonu aktivite tayinleri ile, antimikrobiyal aktivite tayini broth dilüsyon yöntemi ile; sitotoksitite çalışmalar ise Caco-2 kolon kanseri ve 3T3 hücre hatlarında gerçekleştirilmiştir. Sonuçlar Hypericum perforatum L. sonuçları ile karşılaştırılmıştır.