Acipenser Baerii in Environmental Condition Iran

Feasibility studied on production brood stocking Acipenser baerii in environmental condition Iran

Item Type monograph

Authors Yazdani Sadati, Mohammad Ali; Mohseni, M.; Pourali, H.R.; Shakourian, M.; Pour Asadi, M.; Yousefi Jourdehi, A.; Halajian, A.; Sayed Hassani, M.H.; Pourdehghani, M.; Bahmani, M.; Kazemi, R.

Publisher Iranian Fisheries Science Research Institute

Download date 24/09/2021 04:11:57

Link to Item http://hdl.handle.net/1834/39838 –

: (Acipenser baerii ) : 43248

( Acipenser baerii ) : 122 12 86070 : : / :( ) : / :( ) :( ) :( ) : 86 10/ 1/ : 5 : : 1393 : . .

« / » ( Acipenser baerii ) :

122 12 86070 :

92 26/4/ : 43248 :( )

. 18 91 28/8/

█

« » 1 ...... 2...... 1 ....3 ...... 11 3...... 21 8 ...... 2 8 ...... 1: 12 11 ...... 2: 22 15 ...... 3: 32 20 ...... 3 20 ...... 13 32 ...... 23 44 ...... 4 54 ...... 58 ...... رانه .../١

( ) 100 . 4 . 5/243/5

( 17 α- h ydroxyprogestrone) ) 17 α-OHP Estradiol (E 2) Testeosterone (T) (11-Ket) (Tetostesterone ) .

( E2) . IV 11-KT T . III IV II (. P< 05/0 ) 17 α-OHP . IV . 10 31 90 . 69 GV 45 19500 . 10500 8500 60 . 10 3/ 8/6 . . 5 :

٢ / ارش حه

1 1970 . 15000 1991 33000 ( FAO, 2010 ) 385 2009 . ( ) . ( 2009 ) 32576 2009 400 1980 . ) 1389 1500 ( . 100 . . . . . . . . 18 17 14 11 رانه .../٣

. (Acipenser stallatus ) (Acipenser nudiventris ) ( Huso huso ) ( Acipenser persicus ) .

: 11 1 2 3

Acipenseriforms 4 Acipenseridae .( Bemis et al., 1997 ) 27 . Scaphirhynchus Huso Acipenser Pseudoscaphhirynchus . ( Acipenser baerii Brandt, 1869) Acipenser ( Dadswell et al., 1984 ) Kingdom Animalia Phylum Chordata Subphylum Vertabrata Superclass Gnathostomata Class Osteichthyes Subclass Actinopterygii Infraclass Chondrostie Order Acipenseriformes Family Acipenseridae Subfamily Acipenserinae Genus Acipense r Species Baerii (Brandt) ٤ / ارش حه

. 32 62 10 12 . 5 . 167 . .( FAO, 2009 ) . Acipenser baerii baerii, Acipenser baerii baikalensis, Acipenser baerii stenorrhynchus (Anon., CITES (Sokolov and Vaasiev, 1989). . 2000). ( Ob ) . . . 65 210 2 . 60 . 50 . .( FAO, 2009 ) 100 150 20 CITES UNEP-WCMC . .( Ruban, 2002) . 170 200 (Ruban , 2006). 1961 . ( Irtish ) ( Ob ) Lebedev . Akimova Mulitin Sokolov . . رانه .../٥

(Sokolov and Vasilev, 1989). (Ruban and Konoplja. 1994). . (FAO , 2009) ( ) .( Ruban, 2005 ) . . (Sokolov and vasilev, 1989; Ruban, 2006 ) . 10 12 109 ( ) . . 87 43 .( Sokolov and Vasilev, 1989 ) . 189 53 .( CITES ) 32 . 15/1

10 11 40 12 13 .(Sokolov and Vasilev, 1989 Egrov, 1961 ) .( Ruban, 2001) 86 . 832000 2111000 . 60000 . 20000 . . 16500 13600 . 10/8 25 37/2 97/2 . . ٦ / ارش حه

19/5 82/25/2 . (Sokolov and Vasilev, 1989 ) 18/5 . 1940 . 1970 . 1990 .( Williot et al., 2001 ) FAO, ) Acipenser naccarri .( 2009 Acipenser baerii

250

200 150 100 50 0 2005 2006 2007 2008 2009 2010

1: 2005 2006 2007 2008 2009 2010 ( ) 54 146 86 75 114 194 رانه .../٧

( ) 5/5 (Adamek et al ., 2007) 74 (. Sokolov and Vasilev, 1989 ) 25/1 Akimova, ) 97 109 . .( 1980 30 25 10 (. Sokolov and Vasilev, 1989 ) . ( ) 35/1 . 50 80 .( FAO, 2009 ) ( Kolksal et al., 2000) .( Williot et al., 2001 ) 8/6 65 (. Doroshov et al., 1997 ) . CITES . ) 433 360950 283 1998 30000 2000 . ( . ٨ / ارش حه

2 : 1: 2: 3:

1: 12

: 1

. 1390 1387 . . 2 . 1000 100 1 800 200 . 5 1500 10 100 . 5/0 2×2 . . . 1 240 . 6 5/2 60 1 8 1 2 4 6 4 . 2/1 12 4 رانه .../٩

8 5/0 ( Yazdani et al., 2009 ) 10 12(. ) 200

2 ١٠ / ارش حه

: 3 10 18 20 18 16 40 45 . . 22 20 . E C 23 18 . 39 3 . 31 1 4 . . 24 7 150 200 500 10 3(. ) 3 رانه .../ ١١

: %BWI = 100 × (BWf - BWi)/BWi (Hung et al., 1989) BWI = ( ) BWF = ( ) F.C.R = F/(Wt-W0) ( Ronyai et al., 1990) (Abdelghany & Ahmad, 2002) F= W0= ( ) Wt= ( ) G.R = (BWf - BWi) / n (Hung, et al., 1989) BWI = ( ) BWF = ( ) n= %BWI = 100 × (BWf - BWi)/BWi (Hung et al., 1989) BWI = ( ) BWF = ( )

S.G.R = (lnWt -lnW0 )/t ×100 (Ronyai et al., 1990) W0= ( ) Wt= ( ) T= ( )

: 2 22 . ١٢ / ارش حه

) . ( . 1 . 107 150 –200 . 4 3 . ( ) . 3 . 5 4(. ) 5

4 . رانه .../ ١٣

2 . . . 5(. )

5 ) . ( 37 +5 15 + . ١٤ / ارش حه

( ) 1 ( 96 80 70 50 ) . – 1 2 . ( ) 3 37 56 12 . 4 . 5 5 1512 37 . . 5 . (H&E – ) 6 . 7

10 . E600 . 8 ( Caudal vein ) 2 cc . 5 cc . رانه .../ ١٥

2 cc . 9 3000 5 ( Labofuge200 Heraeus sepatech ) . .(Barannikova et al., 20 2004, Sisneros et al., 2004; Guerriero et al., 2005) 17 17 10 17 IM1119 Immunotech (T) IM1452 Immunotech (17 α-HP)

A21854 Immunotech ( ( E2 ) 17 (I125) 125 ) Radioimmunoassay LKB (ng/mL) ( LKB . I125 . (11-KT) 11 11 582751 Cayman 11 11 ( ) 11 ELISA (ng/ml) . SLT

3: 32

GV 30 . . 35 ١٦ / ارش حه

GV . a PI = ⋅100 a (Polarization Index) PI . b . b ( ) GV

IV 10 ( GV ) 16 LHLH 10 I ) 6(. ) 5 ( 90 II 6 5/0 50 . . 50 . رانه .../ ١٧

. . . Rebling Nr.9610003 .Type – 13 ) ( ) ( 40 ( ) ( ) pH 10 ( 5 5000 ) . 2 10 5 . ( 10 ) . . 45 3 15 ( 750 ) 5/04/0 10 . 16 . 100 ( 3:30 ) . % 5 . MST800 1/0 ( D0006 A&D ) 8.( ) . ( ) ١٨ / ارش حه

4 20 7/0×2 .( 9 ) 5/0 300 20 15 4 ( 30 20 ) 60 50 . 100 10 . ( ) 30 25 . 80 9 8 4 Repeated measures . between subject within-subject . Bonferroni . Paired T-test . . ( IV III II ) رانه .../ ١٩

. Two-way ANOVA Tukey . ( Version 13, Inc.,Chicago, IL, USA )SPSS . . ٢٠ / ارش حه

13 16 8/ ± 176/1 ± 8/0 2/7 ± 255/0 1/ ± 2/1 . 16 18/ ± 1/1 . 6/6 ± 265/0 5/ ± 6/82/1 ± 245/0 7/ ± 2/1 72/75/8 123(. () P>0/05 ) . 1/8 ± 102/0 5/ ± 1/83/0 ± 5/0 1387 30 25 25.1± 0.9 24.1 ± 1.1 24.3 ± 0.85 20 21.3± 0.95 21.4 ± 0.8 15 15.9± 1.1 18.1± 1.3 14.5± 0.75 10 11.9± 0.8 10.9± 0.8 (() 9.3± 0.55 5 7.2± 0.4

0 1 2 3 4 5 6 7 8 9 101112

رانه .../ ٢١

30 25 24.7± 1.5 24.4± 1.3 20 22.8± 1.1 20.4± 0.9 20.1± 1.1 15 17.4± 1.1 17.2± 0.5

10 13.2± 1.2 11.5± 0.43 8.6± 0.23 (() 11.5± 0.55 5 11.1± 0.85 0 1 2 3 4 5 6 7 8 9 101112

: 2

30 25 25.7± 1.3 26.5± 1.4 20 23± 0.9 21± 0.5 21± 0.8

15 12± 1.3 14.5± 0.9 8.2± 0.34 10 12.1± 0.8

(() 9.5± 0.38 9.1± 0.54 5 6.6± 0.5

0 1 2 3 4 5 6 7 8 9 101112

3: ٢٢ / ارش حه

( ) . )49( 3/7 . 45 54/ /0 417 . 07/5 /0 055 4/1 . 43/7 رانه .../ ٢٣

3000.0

2500.0

2000.0

1500.0 () 2440.5 ± 61.5 2565.5 ± 51.2 1000.0 1448.8 ± 53.2 2101.3 ± 58.2 500.0 0.0 1 2 3 4

: 4

30000.0

25000.0

20000.0 24405 ± 615 256450 ± 512 15000.0 21012.5 ± 580.5 () 10000.0 14447.5 ± 430.2

5000.0

0.0 1 2 3 4

٢٤ / ارش حه

S.G.R Temperature ( c) 30.000 24.5 ± 1.5 25.000 18.5± 1.2 ±53.2 20.000 15.6 ± 1.3

15.000 9.5 ± 0.9 10.000

0.417± 0.015 0.055± 0.001 0.346± 0.012 0.166± 0.014 5.000 ±53.2 ±53.2 ±53.2 ±53.2

0.000 1 2 3 4

6: Growth rate (gr/day) temperature (c)

30.0 24.5 ± 1.5 25.0 18.5± 1.2 20.0 ±53.2 15.6 ± 1.3 15.0 9.5 ± 0.9 7.3± 0.81 ±53.2 10.0 4.3± 0.61 3.8± 0.23 ±53.2 ±53.2 5.0 1.4± 0.015 ±53.2 0.0 1 2 3 4

: 7

رانه .../ ٢٥

BWI (%)

Temperature ( c) 50.00 45.00 36.62± 1.8 45.54± 0.81 40.00 ±53.2 ±53.2 35.00

30.00 24.5 ± 1.5 25.00 18.5± 1.2 16.9± 1.1 20.00 ±53.2 ±53.2 15.00 9.5 ± 0.9 10.00 15.6 ± 1.3

5.00 5.07± 0.45 0.00 ±53.2 1 2 3 4

: 8

F.C.R 30.00 Temperature (c) 24.5 ± 1.5 25.00 18.5± 1.2 ±53.2 20.00 15.6 ± 1.3

15.00 9.5 ± 0.9 10.00

5.02±0.85 7.43±0.95 2.1±0.06 2.48±0.13 5.00 ±53.2 ±53.2 ±53.2 ±53.2

0.00 : 9 ٢٦ / ارش حه

2/6 4 59 45/ /0 194 . . 37/3 . 19 4/ 37 04/ . 11 98/ 20 4/ . . ﻧﻤﻮدار وزن ﺑﺪن ﺗﺎﺳﻤﺎﻫﻲ ﺳﻴﺒﺮي در ﻃﻮل دوره ﭘﺮورش

4500 4000 3148.3± 235.2 ±53.2 3500 4088.3± 246.2 ±53.2 3000 2949.3± 112.5 ±53.2 2500

() 2000

1500 1000 500 0 0 3TH 6TH : 10 رانه .../ ٢٧

25 37.04± 2.1 ±53.2 20

BWI ( %day) ( BWI 15 19.6±1.5 10 ±53.2

0 0 6TH 12TH

BWI ﻣﻴﺰان درﺟﻪ ﺣﺮارت ودرﺻﺪ اﻓﺰاﻳﺶ وزن ﺑﺪن ﺗﺎﺳﻤﺎﻫﻲ ﺳﻴﺒﺮي در دوره ﻫﺎي ﺷﺶ ﻣﺎﻫﻪ ﭘﺮورش

Tmperature (C) 40 37.04± 2.1 ±53.2 35

25 19.6±1.5 20 ±53.2

15 20.48± 1.8 10 ±53.2 11.98± 0.5 5 ±53.2

0 temperature : 12 ٢٨ / ارش حه

85/5 3/5 . 34 38/ . /2 911 . /0 123 . IV III

6000 5388± 221.5 ±53.2 5000 4088± 215.2 ±53.2 4000

3000 () 2000

1000

0 Oth 12th

: 13

رانه .../ ٢٩

. 08/46/5 13/2 . 35/68/5 99/4 26/4 . . 91/2 37/3 . /0 123 /0 194 25/0 34 38/ 25 85/ .( 59 41/ ) .

6000.00 5388 ± 221.5 5000.00 4088.3 ± 246.2 4000.00

3000.00 2564.5 ± 51.2 () 2000.00

1000.00

0.00 1 2 3

14:

٣٠ / ارش حه

7.00 6.35 ± 0.52 5.85 ± 0.68 6.00

5.00 4.19 ± 0.39 4.00 3.00 2.00 (() 1.00

0.00 1 2 3

15:

4.50 4.00 3.376 3.50 4.19 ± 0.5 2.917245186 3.00 3.37 ± 0.35 2.50 2.91 ± 0.28 2.00 1.50 ( 1.00 0.50 0.00 1 2 3

16: رانه .../ ٣١

0.30

0.25

0.20 0.25 ± 0.041 0.15 0.194 ± 0.021 0.10 0.12 ± 0.0035 0.05 (() 0.00 1 2 3

17:

70.00

60.00

50.00

40.00

30.00 59.455 ±6.1

20.00 34.38 ±3.8 25.85 ±3.2 (%) 10.00

0.00 1 2 3

18: ٣٢ / ارش حه

23 : II . 31( a ) . . . . I II . III 71 43/ . ( Vitellogenesis ) III . .( 31 b,c ) . . . . . . . II . . IV 4 28 57/ رانه .../ ٣٣

IV IV 31( d ) III III . . 6 . (External Zona Radiata) (Basal layer) (Teca layer) . (Pigments) (Fat layer) (Intertnal Zona Radiata) IV 31

(a) (b)

oc cy nu n gl yp ve n (c) yp ve .

: II (a) .( IV III II ) 31: 6 : IV (d) . : III (b,c) . = ve = gl = oc = cy = nu = n . = yg = bl = zri = zre = yp . = tl ٣٤ / ارش حه

. 31.( ) 421 47/ ±77 81/ IV 230 15/ ±21 47/ II

. ( Acipenser baerii ) . . ± : : II .( 32 a ) . . . III 55 6/ . . :III . ( ) . رانه .../ ٣٥

III . . .( 32 b ) . IV 44 4/ ( ) ( ) ( ) : IV .( 32 c ) . . (a) (b) bv

sc sc

sd sd

cs cs

csz sz sz

bv bv : II (a) .( IV III II ) : 32 : III (b) . = sc = cs : IV (c) . . = csz = sz = sd = bv ٣٦ / ارش حه

II 11-KT II 11-KT . (P<0.05) III .( 8/6 ± 67/0 47/2 ± 73/0 ng/ml ) 11-KT . (P ≥ 0.24) 11-KT . (P< 0.05) IV 32.( ) 1/8( ± 6/0 ng/ml )

( Acipenser baerii ) 11-KT 32: .* ± . IV III II n= .)3 (P ≥0.05) ns (P<0.05) ( IV III II

III II E2

E2 II . (P< 0.05)

III E2 .( 33 ) 104 ±21 55/ ng/ml

E2 . 34 ±12 50/ ng/ml .( 33 ) ( 102 66/ ± 33/4 ng/ml )

E2 IV 1

E2 . (II, III) .( 33 ) 12 ng/ml رانه .../ ٣٧

( Acipenser baerii ) E2 : 33 *. ± .( ) IV ( ) III ( ) II ns . (P<0.05) III II n= 1 III II n= 3). (P ≥0.05) .( IV ٣٨ / ارش حه

T . II T II (P=0.82) .( 42 33/ ± 40/4 ng/ml, P<0.05 ) . (P=0.1) III T . ( P=0.75 : ; P=0.13 : ; P=0.52 : ) ( P=0.17 : ; P=0.2 : ; P=0.51 : ) IV 34.( )

( Acipenser baerii ) T 34: *. ± . IV III II n= 3). (P ≥0.05) ns . (P<0.05) II (. IV n= 1 IV III II

( ) II 17 α-OHP ng/ml (P<0.05) ng/ml 17 α-OHP /0 158 ± 04/0 IV .( 35 ) 04/0 ± 01/0 35( ) 16 23 ng/ml . (P=0.5) 17 α-OHP رانه .../ ٣٩

Acipenser ) 17 α-OHP 35: *. ± .( ) IV ( ) II ( baerii n= )3 ns . (P<0.05) (. IV n= 1 II

(P<0.05) III 17 α-OHP 17 α- III 17 α-OHP 36.( ) 4 OHP ٤٠ / ارش حه

± . : 36 n= 3). ns . (P< 0.05) *. (. IV n= 1 II (IV III II) . IV 11-KT 37.( )( 1/8 ± 6/0 ng/ml P<0.05 )

( Acipenser baerii ) 11-KT : 37 n= )3 . ± .(

رانه .../ ٤١

P=0.000) E2

E2 . IV II III III II 38.( )( 102 6/ ± 33/4 ng/ml ) III

. ( Acipenser baeri ) E2 : 38 n= )3 . ± .(

. (P=0.01) 17 α-OHP T T 3 ) 67( ± 5/2 ng/ml ) IV T 9.(

Acipenser ) T (ng/ml) 39: . ± (. baerii . n .3= ٤٢ / ارش حه

. (P=0.02) 17 α- OHP 18/0( ± /0 047 ng/ml ) 17 α- OHP 10.( 3 ) IV

(ng/ml) 17 α- OHP : 10 3 . ± (. Acipenser baerii ) . n .3= 17 α-OHP T II T . II /0 037 ± /0 017 ng/ml 17 α-OHP 12 ± 08/2 ng/ml 39 ) (T=67±2.5 ng/ml, 17 α-OHP=0.18±0.047 ng/ml) IV 10.( 3 3: . 9 6 GV 90 . 5/4 5/7 750 . 7/5 10 7/ . 400 55 . 8/2 4/2 . 8 7 16 . 97 رانه .../ ٤٣

20 . 19500 . . 16 57 20 . 20 ( )3 . 25 . 60 45 22 . 40 30 ( ) . 10500 8500

4 . 10 3/ 8/6 33(. )

٤٤ / ارش حه

4 : 1 . . . . . 24 16 10 30 10 . 30 . . . 24 5/ /0 417 3/7 15 6/ . . 5/9 . 88/0 25 15 1991 Kaushik . (Yazdani et al., 2011) 17 5/ . . 12 10 78 45 54/ 3/7 . 6 . Deng . 19 376/ 04/ 10 63/ 41 08/ رانه .../ ٤٥

(2002 ) 2001 Billard Chebanov . ( 2004 ) Feist . . . 47 . (Brechevski et al., 1983 ) 97 109 .( Akimova, 1980 ) 10 . Sokolov and Vasilev, ) 30 25 .( 1989 : . . . (Brett, 1979; Bertt & Groves, 1979). 13 14 . 13 1700 1991 Kaushik Medale 90 400 . 5/0 55/13/1 1989 Kaushik Luo . 17 5/ ( E.Coioides ) 2006 . . FCR . ٤٦ / ارش حه

. (Nafusi Bahabadi and Soltani, 2008 ) (. Hung et al., 1993; Eroldogan et al.,2004) 1 . 2: 1 (17 α-OHP T; E2;11- KT) . ( IV III II ) ( Acipenser baerii ) II .( Biswass, 1993) (Huso huso × Acipenser ruthenus 2005 Omoto . ( Lateolabrax maculates ) 2002 Lee Yang . II II 1999 Msiska Xasta-Pierce . III Malawi ( Oreochromis karongae ) III . . . 997 Doroshov Acipenser gueldunstaedtii 1993 Detlaff . IV IV ( Acipenser transmontanus ) (Linares-Casenava et al., 2003). رانه .../ ٤٧

Dahle . II 2003 ( Gadus marhua L. ) . II . (III )Spermatidogenesis III Oreochromis karongae . ( IV ) Spermiogenesis Acipenser brevirostrum .( Msiska & Costa-Pierce, 1999 ) IV 2007 Wildhaber . Missouri ( Hurvitz et al ., 2008 ) A.gueldenstardtii III II : IV . IV III II 2008 Hurvitz . II ( A.gueldenstardtii ) IV III . III . IV-c ) 9 II III III ( . IV-a ( 9 ) II . III 1995 Doroshov Vitelogenin . III II . ( IV ) Huso huso × 1996 Amini . ٤٨ / ارش حه

III 5 II ( Acipenser ruthinus) . III ( ) ( IV ) . IV-b 9( ) II Oreochromis karongae . 2 III 2 . (Msiska & Costa-pierce, 1999). ( (Turner %Robinson, 1991). 5/23 Oreochromis karongae Williot ) 86 65 ( et al., 2001 5 4 . ( Holcik, 1989 ) ( Nagahama & Yanashita, 2008 ) 2003 Dahla ( Gadus morhua L. ) III . III II Lecuciscus cephalus . ( V ) . II ( III ) Lepisosteus platyrhincus .( IV ) .( Orland et al ., 2007 )( II II ) II 1997 Doroshov . 9( )2 IV IV Acipender transmontanus . Rhombosolea tapirina 1999 Pankhurst Barnett 250 ≤ D ≤150 II . . 50 IV 250 ≤ D ≤450 III رانه .../ ٤٩

II ( Garcia- Lopes et al., 2007 ) Solea senegalensis 458 ± 5/4 IV 309 ± /3 8 III 101 ± 3/2 ≤ D 282 II . . 394 IV-a 292 ≤ D ≤394 III 144 ≤ . IV III .( Garcia-Lopes et al., 2007 ) : IV II 11-KT IV II (P<0.05) .( 1/8 ± 8/66/0 ± 67/0 : ) III . 11-KT . (P ≥ 0.05) . 11-KT IV ( 1/8 ± )6/0 . ( 07/5 ± 54/0 ) II T . . ( ) II T ( 42 33/ ± 22/4 ) T T IV III (Halobatrachus didactylus )toadfish T . .(Modesto & Canario, 2003) (I, II) T . (Callard et al ., 1976; kuchling et al ., 1999) ٥٠ / ارش حه

IV T . (Bukovskata and Bayunova 1989). vT . 14 2/ ± 5/2 13 1/ ± 9/2 72 T . (. Kuchling et al ., 1999) Perca fluviatilis T Rudriguez et al ., 2001 . ( Sulistyo et al ., 2000) . T.horsfieldi T ( 2003 ) Lagard T T . (Dabrowski et al ., 1996) T . (Kime,1993) IV . (Kime, 1993)

T E2 steroidogenesis T E2 IV E2 . . (Staub & De Beer, 1997) 38 33/ III T 109 )7/ Giant sturgeon (Largarde et al ., 2003) ( 27 41/ ) 161 )7/ (Barannikova et al ., 2004) ( (Semenkova et al ., 2002) ( 166 )6/ (Webb et al., 2001) ( 80 ) (Barannikiva et al ., 2006) ( 184 )8/ . (Scott et al ., 1989) ( T Perca fluviatilis ( 2000 ) Sulistyo . ( III ) رانه .../ ٥١

T 11-KT T . ( Barannikova et al ., 2004) . (Barannikova et al ., , 2004) IV III . II 17 α-OHP ( ) 17 α-OHP . . 39 07/ II ( ) Agu L.moculates 17 α-OHP L.moculates ( ) Nov . (Lee & Yang, 2002) . II 17 α-OHP 17 α-OHP IV . II 03/0 17 α-OHP . IV 18/0 2/3 (Semenkova et al ., 2002) A.stellatus 19/0 : IV 17 α-OHP . (Mylonas et al .,1997) Morone saxatilis (Pankhurst et al ., 1999) Acanthochromis polyacanthanus (Scott et al ., 1998) Pleuronectes platessa . (Lee & Yang, 2002) Lateolabrax maculates Sanferancisco Prochilodus argentheus Arantes 17 α- T . III II 17 α-OHP OHP II 17 α-OHP . IV 17 α-OHP . (kime, 1993) 17,20 βDHP 17 α-OHP . (Suresh et al ., 2000) (MIS) 17 α-OHP ٥٢ / ارش حه

(Nagahama et al ., 1994; Carolsfeld et al .,. 1996; Sulistyo spermiation (Kime, 1993) (Nagahama et al ., 1994) 17 α-OHP . et al ., 2000) . 17 α-OHP (II, III) 17 α-OHP (Nagahama, 1994) . (Doroshov et al ., 1995)

رانه .../ ٥٣

. .

. . . . . ) . ( .

٥٤ / ارش حه

. . . . . . . - .( 1384 a) . . . . . . . . . . . . . - (Acipenser stellatus ) .( 1384 b). 31 . 1384 4. . . . . - . 1390 . . . . . . . . . - . 1390 . . . . . GnRH

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ABSTRACT This sturdy was carried out in three phases including evaluation of growth factors, determination of sexual hormone levels, caviar obtaining and reproducing of Siberian sturgeon ( Acipenser baerii) brood stocks with the aims of brood stocks school production during 4 years. A total of 100 fingerlings with mean weight 1 kg were selected and reached to 2.5, 4 and 5.3 during 3 years, respectively. Steroid hormone levels such as testosterone

(T), 11keto - testosterone (11- KT), estradiol (E 2) and 17 α-hydroxyprogestrone (17 α-OHP) were measured for sexual development studying in males and females at different seasons during 3 years. Results showed that the most levels of 11- KT, E 2 and T were observed at stage IV, III in summer and stage IV of sexual maturation, respectively, that was increased with sex development both in males and females. T levels showed significant difference between males and females (P< 0.05). At the third year of rearing, 31% of females were matured and 10 fish that their GV was between 6 to 9 were selected and injected with LHRH hormone. More than 90% of breeders had possitive response to hormone injection and 19500 larvae were produced from each fish. Larvae converted into fingerlings during 45 – 60 days. 8500 – 10500 fingerlings were produced from each breeder. Also some fish used for caviar obtaining. Results showed that the amount of caviar obtained from each fish was from minimum 6.8 % to maximum 10.3 % of body weight in Acipenser baerii .

Key words: Siberian sturgeon, Brood stocks, Growth, Steroid hormones, Reproduction, Fingerlings and Caviar

Ministry of Jihad – e – Agriculture AGRICULTURAL RESEARCH, EDUCATION & EXTENSION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION – International Sturgeon Research Institute

Project Title : Feasibility studied on production brood stocking Acipenser baerii in environmental condition Iran Approved Number: 2-12-12-86070 Author: Mohammad Ali Yazdani Sadati Project Researcher : Mohammad Ali Yazdani Sadati Collaborator(s) : Mahmoud mohseni, Hamid Reza Pourali, Mahmoud Shakourian, Mohsen PourAsadi, Ayoub yousefi Jourdehi, Ali Halajian, Mir Hamed Sayed Hassani, Mohamad Pourdehghani, Mahmoud Bahmani, Rezvanollah Kazemi Advisor(s): Supervisor: Location of execution : Guilan province Date of Beginning : 2008 Period of execution : 5 Years Publisher : Iranian Fisheries Research Organization Date of publishing : 2014

MINISTRY OF JIHAD - E - AGRICULTURE AGRICULTURAL RESEARCH, EDUCATION & EXTENSION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION - International Sturgeon Research Institute

Project Title : Feasibility studied on production brood stocking Acipenser baerii in environmental condition Iran

Project Researcher : Mohammad Ali Yazdani Sadati