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Human Cell Cycle Regulation Panel, 96

Panel of prevalidated qPCR assays for gene expression profiling of human genes involved in cell cycle regulation

Cat. No. 05 339 359 001 2 plates, each containing 96 assays Version November 2008 N Store at +2 to +8°C N Store protected from light!

Plate Layout

123456789101112 A 1 2 3 4 5 6 7 8 9 10 11 12 ABL 1 ANAPC2 ANAPC4 ATM ATR BAX BCCIP BCL2 BIRC5 BRCA1 BRCA2 CCNA2 B 13 14 15 16 17 18 19 20 21 22 23 24 CCNB1 CCNB2 CCNC CCND1 CCND2 CCND3 CCNE1 CCNE2 CCNF CCNG1 CCNG2 CCNH C 25 26 27 28 29 30 31 32 33 34 35 36 CCNT1 CCNT2 CDC14A CDC16 CDC2 CDC20 CDC25A CDC25B CDC25C CDC34 CDK2 CDK4 D 37 38 39 40 41 42 43 44 45 46 47 48 CDK5R1 CDK6 CDK7 CDK8 CDKN1A CDKN1B CDKN2A CDKN2B CDKN3 CHEK1 CHEK2 CKS1B E 49 50 51 52 53 54 55 56 57 58 59 60 CKS2 CUL1 CUL2 CUL3 E2F1 E2F2 E2F3 E2F4 E2F5 E2F6 GADD45A GTF2H1 F 61 62 63 64 65 66 67 68 69 70 71 72 GTSE1 JUN KNTC1 KPNA2 LGALS3 MAD2L1 MAD2L2 MCM2 MCM3 MYC PCNA PLK1 G 73 74 75 76 77 78 79 80 81 82 83 84 RAD1 RAD17 RAD51 RAD9A RB1 RBL1 RBL2 SKP2 TFDP1 TFDP2 TP53 WEE1 H 85 86 87 88 89 90 91 92 93 94 95 96 ACTB B2M GAPDH HPRT1 RPL13A 18S YWHAZ RT+ RT+ RT+ RT- RT-

H1 – H7: Reference genes H8 – H10: RT positive control H11 – H12: RT minus control Sample Details No. Pos. HGNC- Description No. Pos. HGNC- Description Symbol Symbol 20 B 08 CCNE2 G1/S-specific cyclin-E2. 1 A 01 ABL 1 Proto-oncogene tyrosine-protein kinase ABL1 21 B 09 CCNF G2/mitotic-specific cyclin-F. 2 A 02 ANAPC2Anaphase-promoting complex subunit 2 (APC2) 22 B 10 CCNG1 Cyclin-G1 (Cyclin-G) 3 A 03 ANAPC4Anaphase-promoting complex subunit 4 (APC4) 23 B 11 CCNG2 Cyclin-G2. 4 A 04 ATM Serine-protein kinase ATM 24 B 12 CCNH Cyclin-H (MO15-associated protein) 5 A 05 ATR Serine/threonine-protein kinase ATR 25 C 01 CCNT1 Cyclin-T1 (CycT1) 6 A 06 BAX Apoptosis regulator BAX. 26 C 02 CCNT2 Cyclin-T2 (CycT2) 7 A 07 BCCIP BRCA2 and CDKN1A-interacting protein (Protein TOK- 1) 27 C 03 CDC14A Dual specificity protein phosphatase CDC14A 8 A 08 BCL2 Apoptosis regulator Bcl-2. 28 C 04 CDC16 Cell division cycle protein 16 homolog (CDC16Hs) 9 A 09 BIRC5 Baculoviral IAP repeat-containing protein 5 (Apoptosis 29 C 05 CDC2 Cell division control protein 2 homolog inhibitor survivin) 30 C 06 CDC20 Cell division cycle protein 20 homolog (p55CDC) 10 A 10 BRCA1 Breast cancer type 1 susceptibility protein (RING finger 31 C 07 CDC25AM-phase inducer phosphatase 1 protein 53) 32 C 08 CDC25B M-phase inducer phosphatase 2 11 A 11 BRCA2 Breast cancer type 2 susceptibility protein (Fanconi anemia group D1 protein) 33 C 09 CDC25C M-phase inducer phosphatase 3 12 A 12 CCNA2 Cyclin-A2 (Cyclin-A) 34 C 10 CDC34 Ubiquitin-conjugating enzyme E2 R1 13 B 01 CCNB1 G2/mitotic-specific cyclin-B1. 35 C 11 CDK2 Cell division protein kinase 2 14 B 02 CCNB2 G2/mitotic-specific cyclin-B2. 36 C 12 CDK4 Cell division protein kinase 4 15 B 03 CCNC Cyclin-C (hSRB11) 37 D 01 CDK5R1 Cyclin-dependent kinase 5 activator 1 precursor (CDK5 activator 1) 16 B 04 CCND1 G1/S-specific cyclin-D1 (PRAD1 oncogene) 38 D 02 CDK6 Cell division protein kinase 6 17 B 05 CCND2 G1/S-specific cyclin-D2. 39 D 03 CDK7 Cell division protein kinase 7 18 B 06 CCND3 G1/S-specific cyclin-D3. 40 D 04 CDK8 Cell division protein kinase 8 19 B 07 CCNE1 G1/S-specific cyclin-E1. www.roche-applied-science.com No. Pos. HGNC- Description No. Pos. HGNC- Description Symbol Symbol 41 D 05 CDKN1 Cyclin-dependent kinase inhibitor 1 (p21) 70 F 10 MYC Myc proto-oncogene protein (c-Myc) A 71 F 11 PCNA Proliferating cell nuclear antigen (PCNA) 42 D 06 CDKN1 Cyclin-dependent kinase inhibitor 1B (Cyclin-depen- 72 F 12 PLK1 Serine/threonine-protein kinase PLK1 B dent kinase inhibitor p27) 73 G 01 RAD1 Cell cycle checkpoint protein RAD1 43 D 07 CDKN2 Cyclin-dependent kinase inhibitor 2A, isoform 4 A (p14ARF) 74 G 02 RAD17 Cell cycle checkpoint protein RAD17 (hRad17) 44 D 08 CDKN2 Cyclin-dependent kinase 4 inhibitor B (p14-INK4b) 75 G 03 RAD51 DNA repair protein RAD51 homolog 1 (hRAD51) B 76 G 04 RAD9A Cell cycle checkpoint control protein RAD9A 45 D 09 CDKN3 Cyclin-dependent kinase inhibitor 3 77 G 05 RB1 Retinoblastoma-associated protein (pRb) 46 D 10 CHEK1 Serine/threonine-protein kinase Chk1 78 G 06 RBL1 Retinoblastoma-like protein 1 (107 kDa retinoblastoma- 47 D 11 CHEK2 Serine/threonine-protein kinase Chk2 associated protein) 48 D 12 CKS1B Cyclin-dependent kinases regulatory subunit 1 (CKS-1) 79 G 07 RBL2 Retinoblastoma-like protein 2 (130 kDa retinoblastoma- associated protein) 49 E 01 CKS2 Cyclin-dependent kinases regulatory subunit 2 (CKS-2) 80 G 08 SKP2 S-phase kinase-associated protein 2 (F-box protein 50 E 02 CUL1 Cullin-1 (CUL-1) Skp2) 51 E 03 CUL2 Cullin-2 (CUL-2) 81 G 09 TFDP1 Transcription factor Dp-1 (E2F dimerization partner 1) 52 E 04 CUL3 Cullin-3 (CUL-3) 82 G 10 TFDP2 Transcription factor Dp-2 (E2F dimerization partner 2) 53 E 05 E2F1 Transcription factor E2F1 (E2F-1) 83 G 11 TP53 Cellular tumor antigen p53 (Tumor suppressor p53) 54 E 06 E2F2 Transcription factor E2F2 (E2F-2) 84 G 12 WEE1 Wee1-like protein kinase 55 E 07 E2F3 Transcription factor E2F3 (E2F-3) 85 H 01 ACTB Reference Gene: 56 E 08 E2F4 Transcription factor E2F4 (E2F-4) Actin, cytoplasmic 1 (Beta-actin) 57 E 09 E2F5 Transcription factor E2F5 (E2F-5) 86 H 02 B2M Reference Gene: 58 E 10 E2F6 Transcription factor E2F6 (E2F-6) Beta-2-microglobulin precursor 59 E 11 GADD45Growth arrest and DNA-damage-inducible protein 87 H 03 GAPDH Reference Gene: A GADD45 alpha (DNA- damage-inducible transcript 1) Glyceraldehyde-3-phosphate dehydrogenase 60 E 12 GTF2H1 General transcription factor IIH subunit 1 (General tran- 88 H 04 HPRT1 Reference Gene: scription factor IIH polypeptide 1) Hypoxanthine-guanine phosphoribosyltransferase 61 F 01 GTSE1 G2 and S phase-expressed protein 1 (B99 homolog) 89 H 05 RPL13A Reference Gene: 60S ribosomal protein L13a (23 kDa highly basic pro- 62 F 02 JUN Transcription factor AP-1 (Activator protein 1) tein) 63 F 03 KNTC1 Kinetochore-associated protein 1 (Rough deal 90 H 06 18S Reference Gene: homolog) 18S ribosomal RNA 64 F 04 KPNA2 Importin subunit alpha-2 (Karyopherin subunit alpha-2) 91 H 07 YWHAZ Reference Gene: 65 F 05 LGALS3 Galectin-3 (Galactose-specific lectin 3) 14-3-3 protein zeta/delta (Protein kinase C inhibitor protein 1) 66 F 06 MAD2L Mitotic spindle assembly checkpoint protein MAD2A 1 (MAD2-like 1) 92 H 08 RT positive control 5’ 67 F 07 MAD2L Mitotic spindle assembly checkpoint protein MAD2B 93 H 09 RT positive control middle’ 2 (MAD2-like 2) 94 H 10 RT positive control 3’ 68 F 08 MCM2 DNA replication licensing factor MCM2 (Minichromo- 95 H 11 RT minus control, cDNA some maintenance protein 2 homolog) 96 H 12 RT minus control, RNA 69 F 09 MCM3 DNA replication licensing factor MCM3 (DNA poly- merase alpha holoenzyme-associated protein P1)

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