Single-Cell Transcriptomics Identifies CD44 As a New Marker and Regulator of Haematopoietic Stem Cells Development

Supplementary Information Oatley, Vargel-Bölükbası et al.

Single-cell transcriptomics identifies CD44 as a new marker and regulator of haematopoietic stem cells development

Morgan Oatley1*, Özge Vargel-Bölükbası1,2*, Valentine Svensson3,4,5, Maya Shvartsman1, Kerstin Ganter1, Katharina Zirngibl6, Polina V. Pavlovich1,7, Vladislava Milchevskaya6,8, Vladimira Foteva1, Kedar N. Natarajan3,9, Bianka Baying10, Vladimir Benes10, Kiran R. Patil6, Sarah A. Teichmann3 & Christophe Lancrin1,11

1 European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, Monterotondo, Italy. 2 Current address: Boston's Children Hospital/Harvard Medical School, Boston, USA. 3 Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, UK. 4 European Molecular Biology Laboratory, EMBL-EBI, Wellcome Genome Campus, Hinxton, UK. 5 Current address: Pachter Lab, Caltech, California, USA. 6 European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany 7 Moscow Institute of Physics and Technology, Institutskii Per. 9, Moscow Region, Dolgoprudny 141700, Russia. 8 Current address: Institut für Medizinische Statistik und Bioinformatik, Köln, Germany 9 Current address: The University of Southern Denmark, Danish Institute for Advanced Study, Department of Biochemistry and Molecular Biology, Odense, Denmark. 10 European Molecular Biology Laboratory, Genomics Core Facility, Heidelberg, Germany. 11 Correspondence: [email protected] * Co-first authors Supplementary Figure S1: Experimental layout for the experiments for antibody screen and single-cell RNA sequencing

a 1) Differentiation of ESCs into blood b 1) Isolation of the AGM region and cells analysis of VE-Cadherin and CD41 ESCs markers

EB culture for 3 days

AGM 2) Isolation of Flk-1+ BL-CFCs

2) FACS sorting of VE-Cadherin+ cells Haemangioblast AGM E10.5 culture for 1.5 days

105

3) FACS Analysis for VE-cadherin, 104 CD41 and a panel of 176 markers

(BD mouse Lyoplate) Cad - 103 VE APC-A: ve-cadh

102 Cad - 0 VE

2 3 4 5 Antibody 0 10 10 10 10 PE-A:CD41 CD41

4) Identification of forty-two markers 3) Capture of 96 cells on the expressed by VE-Cad+. Sixteen of Fluidigm C1 platform them displayed bimodal expression.

4) Preparation of 96 cDNA libraries and next generation sequencing on Illumina HiSeq.

5) Sequencing analysis, AGM identification of subpopulations and selection of candidate marker genes. 5) Isolation of the AGM region and test the expression of the 16 markers

Oatley, Vargel-Bölükbası et al. 2018 Supplementary Figure S1: Experimental layout for the experiments for antibody screen and single-cell RNA sequencing (a) Strategy used for the antibody screen. (b) Description of the different steps following for the single cell RNA sequencing analysis in the AGM. See also Table S1 and Figure 1.

Oatley, Vargel-Bölükbası et al. 2018 Supplementary Table S1: Results of the antibody screen

Target protein Gene symbol Gene name Bimodal expression CD9 Cd9 CD9 antigen No CD13 Anpep alanyl (membrane) aminopeptidase No CD19 Cd19 CD19 antigen No CD23 Fcer2a Fc receptor, IgE, low affinity II, alpha polypeptide Yes CD24 Cd24a CD24a antigen No CD29 Itgb1 integrin beta 1 (fibronectin receptor beta) No CD31 Pecam1 platelet/endothelial cell adhesion molecule 1 No CD34 Cd34 CD34 antigen Yes CD35 Cr2 complement receptor 2 No CD38 Cd38 CD38 antigen No CD41 Itga2b integrin alpha 2b Yes CD44 Cd44 CD44 antigen Yes CD47 Cd47 CD47 antigen No CD49d Itga4 integrin alpha 4 Yes CD49e Itga5 integrin alpha 5 (fibronectin receptor alpha) No CD51 Itgav integrin alpha V Yes CD54 Icam1 intercellular adhesion molecule 1 Yes CD55 Cd55 CD55 molecule, decay accelerating factor for complement Yes CD61 Itgb3 integrin beta 3 Yes CD62e Sele selectin, endothelial cell No CD71 Tfrc transferrin receptor Yes CD81 Cd81 CD81 antigen No CD93 Cd93 CD93 antigen No CD94 Klrd1 killer cell lectin-like receptor, subfamily D, member 1 No CD98 Slc3a2 solute carrier family, member 2 No CD102 Icam2 intercellular adhesion molecule 2 No CD104 Itgb4 integrin beta 4 No CD106 Vcam1 vascular cell adhesion molecule 1 Yes CD117 Kit KIT proto-oncogene receptor tyrosine kinase Yes CD119 Ifngr1 interferon gamma receptor 1 Yes CD137 Tnfrsf9 tumor necrosis factor receptor superfamily, member 9 No CD138 Sdc1 syndecan 1 No CD144 Cdh5 cadherin 5 No CD147 basigin basigin No CD200 Cd200 CD200 antigen No CD284 Tlr4 toll-like receptor 4 No CD309 Kdr kinase insert domain protein receptor No Crry/p65 Cr1l complement component (3b/4b) receptor 1-like No MadCam1 MadCam1 mucosal vascular addressin cell adhesion molecule 1 Yes Meca32 Plvap plasmalemma vesicle associated protein No PIR-A/B NA NA Yes Sca1 Ly6a/e lymphocyte antigen 6 complex, locus A/E Yes

Oatley, Vargel-Bölükbası et al. 2018 et al. 2018 E10_30_to32sp E10_5_36_to39sp E11_5_42_to45sp E9_5_23_to26sp E10_30_to32sp E10_5_36_to39sp E11_5_42_to45sp E9_5_23_to26sp E10_30_to32sp E10_5_36_to39sp E11_5_42_to45sp E9_5_23_to26sp CD44high CD44lo_Kitneg CD44lo_Kitpos CD44neg P4_AGM_VE.cad_High_CD44_High_40 P4_AGM_VE.cad_High_CD44_High_01 P4_AGM_VE.cad_High_CD44_High_43 P4_AGM_VE.cad_Low_CD44._04 P4_AGM_VE.cad_High_CD44_High_17 P4_AGM_VE.cad_Low_CD44._17 P4_AGM_VE.cad_Low_CD44._19 P4_AGM_VE.cad_High_CD44_High_35 P4_AGM_VE.cad_High_CD44_High_09 P4_AGM_VE.cad_High_CD44_High_19 P4_AGM_VE.cad_High_CD44_High_27 P4_AGM_VE.cad_Low_CD44._21 P4_AGM_VE.cad_Low_CD44._02 P4_AGM_VE.cad_Low_CD44._24 P4_AGM_VE.cad_High_CD44_High_44 P3_AGM_VE.cad_High_CD44_low_24 P4_AGM_VE.cad_High_CD44_High_45 P4_AGM_VE.cad_High_CD44_High_11 P1_AGM_VE.cad._CD44._08 P4_AGM_VE.cad_Low_CD44._10 P4_AGM_VE.cad_Low_CD44._34 P4_AGM_VE.cad_Low_CD44._27 P4_AGM_VE.cad_Low_CD44._32 P4_AGM_VE.cad_High_CD44_High_31 P4_AGM_VE.cad_Low_CD44._11 P4_AGM_VE.cad_Low_CD44._39 P4_AGM_VE.cad_High_CD44_High_20 P4_AGM_VE.cad_Low_CD44._16 P4_AGM_VE.cad_Low_CD44._35 P4_AGM_VE.cad_Low_CD44._41 P1_AGM_VE.cad._CD44._18.1 P4_AGM_VE.cad_Low_CD44._44 P4_AGM_VE.cad_High_CD44_High_10 P4_AGM_VE.cad_Low_CD44._08 P4_AGM_VE.cad_Low_CD44._06 P4_AGM_VE.cad_High_CD44_High_23 P4_AGM_VE.cad_High_CD44_High_25 P4_AGM_VE.cad_Low_CD44._25 P4_AGM_VE.cad_High_CD44_High_15 P4_AGM_VE.cad_High_CD44_High_08 P4_AGM_VE.cad_High_CD44_High_12 P4_AGM_VE.cad_High_CD44_High_05 P4_AGM_VE.cad_Low_CD44._20 P1_AGM_VE.cad._CD44._11.1 P4_AGM_VE.cad_Low_CD44._01 P4_AGM_VE.cad_Low_CD44._07 P4_AGM_VE.cad_Low_CD44._05 P4_AGM_VE.cad_High_CD44_High_07 P4_AGM_VE.cad_Low_CD44._38 P4_AGM_VE.cad_Low_CD44._43 P4_AGM_VE.cad_High_CD44_High_03 P4_AGM_VE.cad_Low_CD44._42 P4_AGM_VE.cad_Low_CD44._14 P4_AGM_VE.cad_High_CD44_High_46 P4_AGM_VE.cad_Low_CD44._33 P1_AGM_VE.cad._CD44._02.1 P4_AGM_VE.cad_High_CD44_High_42 P4_AGM_VE.cad_High_CD44_High_02 P1_AGM_VE.cad._CD44._15.1 P1_AGM_VE.cad._CD44._10.1 P4_AGM_VE.cad_Low_CD44._29 P4_AGM_VE.cad_Low_CD44._28 P4_AGM_VE.cad_High_CD44_High_22 P4_AGM_VE.cad_Low_CD44._13 P4_AGM_VE.cad_Low_CD44._36 P1_AGM_VE.cad._CD44._06 P1_AGM_VE.cad._CD44._01.1 P4_AGM_VE.cad_High_CD44_High_38 P4_AGM_VE.cad_High_CD44_High_13 P4_AGM_VE.cad_Low_CD44._15 P4_AGM_VE.cad_High_CD44_High_18 P4_AGM_VE.cad_High_CD44_High_39 P4_AGM_VE.cad_High_CD44_High_30 P4_AGM_VE.cad_High_CD44_High_21 P4_AGM_VE.cad_High_CD44_High_24 P5_AGM_VE.cad._CD44._10 P5_AGM_VE.cad_High_CD44_low_46 P5_AGM_VE.cad._CD44._36 P6_CD44low_C.kit._45 P5_AGM_VE.cad_High_CD44_low_36 P5_AGM_VE.cad_High_CD44_low_02 P5_AGM_VE.cad_High_CD44_low_19 P5_AGM_VE.cad._CD44._20 P5_AGM_VE.cad._CD44._11 P1_AGM_VE.cad._CD44._05 P1_AGM_VE.cad._CD44._13 P3_AGM_VE.cad._CD44._15 P1_AGM_VE.cad._CD44._09 P3_AGM_VE.cad._CD44._06 P3_AGM_VE.cad._CD44._46 P5_AGM_VE.cad._CD44._07 P5_AGM_VE.cad._CD44._30 P1_AGM_VE.cad._CD44._22 P3_AGM_VE.cad._CD44._28 P3_AGM_VE.cad._CD44._27 P3_AGM_VE.cad._CD44._22 P3_AGM_VE.cad._CD44._42 P1_AGM_VE.cad._CD44._02 P3_AGM_VE.cad._CD44._35 P3_AGM_VE.cad._CD44._20 P3_AGM_VE.cad._CD44._13 P1_AGM_VE.cad._CD44._19 P3_AGM_VE.cad_High_CD44_low_22 P5_AGM_VE.cad_High_CD44_low_45 P1_AGM_VE.cad._CD44._23 P5_AGM_VE.cad_High_CD44_low_22 P5_AGM_VE.cad._CD44._16 P1_AGM_VE.cad._CD44._20 P5_AGM_VE.cad._CD44._44 P5_AGM_VE.cad._CD44._21 P5_AGM_VE.cad._CD44._40 P5_AGM_VE.cad._CD44._23 P5_AGM_VE.cad._CD44._41 P5_AGM_VE.cad_High_CD44_low_23 P5_AGM_VE.cad._CD44._18 P5_AGM_VE.cad._CD44._25 P5_AGM_VE.cad._CD44._26 P5_AGM_VE.cad._CD44._24 P5_AGM_VE.cad._CD44._22 P6_CD44low_C.kit._26 P6_CD44low_C.kit._13 P6_CD44low_C.kit._25 P5_AGM_VE.cad_High_CD44_low_07 P3_AGM_VE.cad_High_CD44_low_06 P6_CD44low_C.kit._03 P6_CD44low_C.kit._41 P3_AGM_VE.cad_High_CD44_low_30 P3_AGM_VE.cad_High_CD44_low_20 P5_AGM_VE.cad_High_CD44_low_10 P3_AGM_VE.cad._CD44._31 P3_AGM_VE.cad_High_CD44_low_45 P1_AGM_VE.cad._CD44._15 P1_AGM_VE.cad._CD44._04 P1_AGM_VE.cad._CD44._14 P1_AGM_VE.cad._CD44._16 P6_CD44low_C.kit._09 P6_CD44low_C.kit._34 P6_CD44low_C.kit._15 P6_CD44low_C.kit._01 P3_AGM_VE.cad_High_CD44_low_46 P3_AGM_VE.cad_High_CD44_low_34 P6_CD44low_C.kit._18 P6_CD44low_C.kit._38 P3_AGM_VE.cad_High_CD44_low_21 P6_CD44low_C.kit._24 P6_CD44low_C.kit._25.1 P6_CD44low_C.kit._11 P5_AGM_VE.cad_High_CD44_low_34 P3_AGM_VE.cad_High_CD44_low_29 P3_AGM_VE.cad_High_CD44_low_05 P3_AGM_VE.cad_High_CD44_low_03 P3_AGM_VE.cad_High_CD44_low_10 P3_AGM_VE.cad_High_CD44_low_33 P3_AGM_VE.cad_High_CD44_low_13 P6_CD44low_C.kit._04 P6_CD44low_C.kit._39 P6_CD44low_C.kit._35 P3_AGM_VE.cad_High_CD44_low_44 P3_AGM_VE.cad_High_CD44_low_14 P3_AGM_VE.cad_High_CD44_low_17 P3_AGM_VE.cad_High_CD44_low_38 P3_AGM_VE.cad_High_CD44_low_12 P1_AGM_VE.cad._CD44._12 P3_AGM_VE.cad_High_CD44_low_25 P6_CD44low_C.kit._29 P6_CD44low_C.kit._30 P6_CD44low_C.kit._40 P6_CD44low_C.kit._22 P6_CD44low_C.kit._46 P6_CD44low_C.kit._20 P5_AGM_VE.cad_High_CD44_low_11 P5_AGM_VE.cad_High_CD44_low_37 P5_AGM_VE.cad_High_CD44_low_29 P6_CD44low_C.kit._43.1 P6_CD44low_C.kit._17 P6_CD44low_C.kit._42 P6_CD44low_C.kit._38.1 P5_AGM_VE.cad_High_CD44_low_16 P6_CD44low_C.kit._06 P6_CD44low_C.kit._12 P5_AGM_VE.cad_High_CD44_low_17 P6_CD44low_C.kit._21 P6_CD44low_C.kit._05 P6_CD44low_C.kit._39.1 P6_CD44low_C.kit._44 P6_CD44low_C.kit._02 P5_AGM_VE.cad_High_CD44_low_12 P6_CD44low_C.kit._27 P6_CD44low_C.kit._10 P5_AGM_VE.cad_High_CD44_low_39 P6_CD44low_C.kit._43 P6_CD44low_C.kit._18.1 P6_CD44low_C.kit._34.1 P6_CD44low_C.kit._32.1 P6_CD44low_C.kit._33.1 P6_CD44low_C.kit._11.1 P6_CD44low_C.kit._30.1 P6_CD44low_C.kit._20.1 P6_CD44low_C.kit._27.1 P5_AGM_VE.cad_High_CD44_low_32 P6_CD44low_C.kit._08.1 P6_CD44low_C.kit._46.1 P6_CD44low_C.kit._05.1 P5_AGM_VE.cad_High_CD44_low_13 P3_AGM_VE.cad_High_CD44_low_18 P6_CD44low_C.kit._03.1 P6_CD44low_C.kit._36.1 P6_CD44low_C.kit._14 P6_CD44low_C.kit._06.1 P6_CD44low_C.kit._42.1 P6_CD44low_C.kit._19 P5_AGM_VE.cad_High_CD44_low_28 P6_CD44low_C.kit._12.1 P6_CD44low_C.kit._41.1 P6_CD44low_C.kit._26.1 P6_CD44low_C.kit._28.1 P6_CD44low_C.kit._01.1 P6_CD44low_C.kit._35.1 P6_CD44low_C.kit._16.1 P6_CD44low_C.kit._45.1 P3_AGM_VE.cad_High_CD44_low_11 P6_CD44low_C.kit._40.1 P6_CD44low_C.kit._02.1 P6_CD44low_C.kit._04.1 P6_CD44low_C.kit._07.1 P6_CD44low_C.kit._29.1 P6_CD44low_C.kit._21.1 P6_CD44low_C.kit._28 P6_CD44low_C.kit._08 P6_CD44low_C.kit._22.1 P6_CD44low_C.kit._37.1 P6_CD44low_C.kit._31.1 P4_AGM_VE.cad_Low_CD44._18 P4_AGM_VE.cad_Low_CD44._46 P4_AGM_VE.cad_High_CD44_High_34 P4_AGM_VE.cad_Low_CD44._03 P4_AGM_VE.cad_Low_CD44._23 P4_AGM_VE.cad_High_CD44_High_14 P4_AGM_VE.cad_Low_CD44._12 P4_AGM_VE.cad_Low_CD44._22 P4_AGM_VE.cad_High_CD44_High_32 P4_AGM_VE.cad_High_CD44_High_28 P6_CD44low_C.kit._07 P3_AGM_VE.cad_High_CD44_low_28 P6_CD44low_C.kit._36 P5_AGM_VE.cad_High_CD44_low_14 P3_AGM_VE.cad_High_CD44_low_04 P6_CD44low_C.kit._16 P3_AGM_VE.cad_High_CD44_low_15 P1_AGM_VE.cad._CD44._17.1 P5_AGM_VE.cad_High_CD44_low_26 P6_CD44low_C.kit._37 P3_AGM_VE.cad_High_CD44_low_07 P5_AGM_VE.cad_High_CD44_low_31 P3_AGM_VE.cad_High_CD44_low_43 P6_CD44low_C.kit._15.1 P5_AGM_VE.cad_High_CD44_low_24 P6_CD44low_C.kit._10.1 P5_AGM_VE.cad_High_CD44_low_09 P6_CD44low_C.kit._17.1 P6_CD44low_C.kit._23.1 P6_CD44low_C.kit._44.1 P5_AGM_VE.cad_High_CD44_low_42 P6_CD44low_C.kit._23 P6_CD44low_C.kit._09.1 P5_AGM_VE.cad_High_CD44_low_18 P6_CD44low_C.kit._31 P3_AGM_VE.cad._CD44._14 P3_AGM_VE.cad._CD44._02 P6_CD44low_C.kit._33 P5_AGM_VE.cad_High_CD44_low_20 P1_AGM_VE.cad._CD44._07 P1_AGM_VE.cad._CD44._03 P1_AGM_VE.cad._CD44._14.1 P1_AGM_VE.cad._CD44._19.1 P1_AGM_VE.cad._CD44._18 P1_AGM_VE.cad._CD44._13.1 P5_AGM_VE.cad._CD44._27 P1_AGM_VE.cad._CD44._12.1 P1_AGM_VE.cad._CD44._10 P5_AGM_VE.cad_High_CD44_low_41 P5_AGM_VE.cad_High_CD44_low_03 P5_AGM_VE.cad._CD44._43 P5_AGM_VE.cad._CD44._37 P5_AGM_VE.cad._CD44._08 P5_AGM_VE.cad_High_CD44_low_08 P5_AGM_VE.cad._CD44._31 P5_AGM_VE.cad._CD44._06 P5_AGM_VE.cad_High_CD44_low_15 P3_AGM_VE.cad._CD44._41 P5_AGM_VE.cad._CD44._38 P5_AGM_VE.cad_High_CD44_low_30 P5_AGM_VE.cad_High_CD44_low_06 P5_AGM_VE.cad_High_CD44_low_33 P5_AGM_VE.cad_High_CD44_low_44 P5_AGM_VE.cad._CD44._04 P5_AGM_VE.cad._CD44._39 P1_AGM_VE.cad._CD44._22.1 P1_AGM_VE.cad._CD44._01 P3_AGM_VE.cad._CD44._03 P3_AGM_VE.cad_High_CD44_low_42 P3_AGM_VE.cad._CD44._04 P5_AGM_VE.cad._CD44._15 P5_AGM_VE.cad_High_CD44_low_40 P6_CD44low_C.kit._24.1 P1_AGM_VE.cad._CD44._05.1 P1_AGM_VE.cad._CD44._21 P5_AGM_VE.cad._CD44._32 P5_AGM_VE.cad_High_CD44_low_38 P5_AGM_VE.cad._CD44._05 P5_AGM_VE.cad._CD44._02 P5_AGM_VE.cad._CD44._01 P5_AGM_VE.cad_High_CD44_low_43 P5_AGM_VE.cad._CD44._03 P1_AGM_VE.cad._CD44._21.1 P3_AGM_VE.cad._CD44._01 P6_CD44low_C.kit._32 P6_CD44low_C.kit._13.1 P1_AGM_VE.cad._CD44._11 P3_AGM_VE.cad_High_CD44_low_39 P3_AGM_VE.cad._CD44._21 P3_AGM_VE.cad._CD44._39 P3_AGM_VE.cad._CD44._44 P5_AGM_VE.cad_High_CD44_low_27 P5_AGM_VE.cad._CD44._09 P5_AGM_VE.cad_High_CD44_low_05 P5_AGM_VE.cad._CD44._29 P3_AGM_VE.cad_High_CD44_low_36 P3_AGM_VE.cad_High_CD44_low_19 P3_AGM_VE.cad._CD44._07 P3_AGM_VE.cad._CD44._26 P3_AGM_VE.cad_High_CD44_low_23 P1_AGM_VE.cad._CD44._17 P1_AGM_VE.cad._CD44._20.1 P1_AGM_VE.cad._CD44._09.1 P3_AGM_VE.cad._CD44._23 P3_AGM_VE.cad._CD44._38 P3_AGM_VE.cad._CD44._36 P5_AGM_VE.cad._CD44._28 P1_AGM_VE.cad._CD44._16.1 P5_AGM_VE.cad._CD44._12 P5_AGM_VE.cad._CD44._34 GroupID GroupID GroupID

GroupID

15 10 5 0 5 0 15 10 15 10 5 0 5 0 15 10 ESAM

ADCY4 NOTCH1

bh1

GDPD5

- KIT

BH1 BH1 BH1 BH1

− − PCDH12 − −

Ptprb Col4a2 Tek Acvrl1 Eng Pecam1 Palld Bmpr1a Acvr1b Lyl1 Smad7 Fbn1 Smad6 Dpysl3 Erg Sox7 Meis2 Npr1 Tgfbr1 Fli1 Smad4 Lmo2 Smad1 Smad5 Smad2 Tal1 Bmpr2 Smad3 Upp1 Cdh2 Pcdh12 Kit Gdpd5 Notch1 Adcy4 Esam Lad1 Bmp4 Lat Samd4 Snai1 Pdzd2 She Tgfb2 Atp2a3 Myb Gata2 Gfi1 Itgb3 Fmo1 Met Serpine1 Myom1 Epor Mpo Gfi1b Gata1 Epo Thpo Lgr5 Smad9 Gria4 Tgfb3 Cldn5 Cacna2d1 Flrt2 Hbb Sash1 Snai2 Acta2 Col4a5 Gpr126 Itgam Sla Enpp1 Eps8 Ptprc Runx1 Spi1 Ppia Ctnnb1 Tgfb1 Cdh5 Kdr Ramp2 Ptprm Acvr2a Fam122b Acvr2b Ppp1r16b Acvr1 Plcd1 Tgfbr2 Dcaf12l1 Itga2b FLI1 SMAD4 LMO2 SMAD1 SMAD5 SMAD2 TAL1 BMPR2 SMAD3 UPP1 CDH2 PCDH12 KIT GDPD5 NOTCH1 ADCY4 ESAM CDH2 GFI1B GATA1 EPO THPO LGR5 SMAD9 GRIA4 TGFB3 CLDN5 CACNA2D1 FLRT2 HBB SASH1 SNAI2 ACTA2 COL4A5 GPR126 ITGAM SLA ENPP1 EPS8 PTPRC RUNX1 SFPI1 PPIA CTNNB1 TGFB1 CDH5 KDR RAMP2 PTPRM PTPRB COL4A2 TEK ACVRL1 ENG PECAM1 PALLD BMPR1A ACVR1B LYL1 SMAD7 FBN1 SMAD6 DPYSL3 ERG SOX7 MEIS2 NPR1 TGFBR1 GroupID ACVR2A FAM122B ACVR2B PPP1R16B ACVR1 PLCD1 TGFBR2 DCAF12L1 ITGA2B LAD1 BMP4 LAT SAMD4 SNAI1 PDZD2 SHE TGFB2 ATP2A3 MYB GATA2 GFI1 ITGB3 FMO1 MET SERPINE1 MYOM1 EPOR MPO KIT GDPD5 NOTCH1 ADCY4 ESAM PECAM1 PALLD BMPR1A ACVR1B LYL1 SMAD7 FBN1 SMAD6 DPYSL3 ERG SOX7 MEIS2 NPR1 TGFBR1 FLI1 SMAD4 LMO2 SMAD1 SMAD5 SMAD2 TAL1 BMPR2 SMAD3 UPP1 CDH2 PCDH12 FLRT2 HBB SASH1 SNAI2 ACTA2 COL4A5 GPR126 ITGAM SLA ENPP1 EPS8 PTPRC RUNX1 SFPI1 PPIA CTNNB1 TGFB1 CDH5 KDR RAMP2 PTPRM PTPRB COL4A2 TEK ACVRL1 ENG SAMD4 SNAI1 PDZD2 SHE TGFB2 ATP2A3 MYB GATA2 GFI1 ITGB3 FMO1 MET SERPINE1 MYOM1 EPOR MPO GFI1B GATA1 EPO THPO LGR5 SMAD9 GRIA4 TGFB3 CLDN5 CACNA2D1 GroupID ACVR2A FAM122B ACVR2B PPP1R16B ACVR1 PLCD1 TGFBR2 DCAF12L1 ITGA2B LAD1 BMP4 LAT KIT GDPD5 NOTCH1 ADCY4 ESAM ENG PECAM1 PALLD BMPR1A ACVR1B LYL1 SMAD7 FBN1 SMAD6 DPYSL3 ERG SOX7 MEIS2 NPR1 TGFBR1 FLI1 SMAD4 LMO2 SMAD1 SMAD5 SMAD2 TAL1 BMPR2 SMAD3 UPP1 CDH2 PCDH12 CACNA2D1 FLRT2 HBB SASH1 SNAI2 ACTA2 COL4A5 GPR126 ITGAM SLA ENPP1 EPS8 PTPRC RUNX1 SFPI1 PPIA CTNNB1 TGFB1 CDH5 KDR RAMP2 PTPRM PTPRB COL4A2 TEK ACVRL1 BMP4 LAT SAMD4 SNAI1 PDZD2 SHE TGFB2 ATP2A3 MYB GATA2 GFI1 ITGB3 FMO1 MET SERPINE1 MYOM1 EPOR MPO GFI1B GATA1 EPO THPO LGR5 SMAD9 GRIA4 TGFB3 CLDN5 GroupID ACVR2A FAM122B ACVR2B PPP1R16B ACVR1 PLCD1 TGFBR2 DCAF12L1 ITGA2B LAD1 SMAD4 LMO2 SMAD1 SMAD5 SMAD2 TAL1 BMPR2 SMAD3 UPP1 CDH2 PCDH12 KIT GDPD5 NOTCH1 ADCY4 ESAM EPO THPO LGR5 SMAD9 GRIA4 TGFB3 CLDN5 CACNA2D1 FLRT2 HBB SASH1 SNAI2 ACTA2 COL4A5 GPR126 ITGAM SLA ENPP1 EPS8 PTPRC RUNX1 SFPI1 PPIA CTNNB1 TGFB1 CDH5 KDR RAMP2 PTPRM PTPRB COL4A2 TEK ACVRL1 ENG PECAM1 PALLD BMPR1A ACVR1B LYL1 SMAD7 FBN1 SMAD6 DPYSL3 ERG SOX7 MEIS2 NPR1 TGFBR1 FLI1 GroupID ACVR2A FAM122B ACVR2B PPP1R16B ACVR1 PLCD1 TGFBR2 DCAF12L1 ITGA2B LAD1 BMP4 LAT SAMD4 SNAI1 PDZD2 SHE TGFB2 ATP2A3 MYB GATA2 GFI1 ITGB3 FMO1 MET SERPINE1 MYOM1 EPOR MPO GFI1B GATA1 Groups Embryo stages

P6_CD44low_C.kit._07 Bölükbası P3_AGM_VE.cad_High_CD44_low_28P3_AGM_VE.cad_High_CD44_low_28P6_CD44low_C.kit._07 UPP1 P6_CD44low_C.kit._07P6_CD44low_C.kit._07 - P3_AGM_VE.cad_High_CD44_low_28P3_AGM_VE.cad_High_CD44_low_28 P5_AGM_VE.cad_High_CD44_low_14P6_CD44low_C.kit._36P6_CD44low_C.kit._36 P6_CD44low_C.kit._36P6_CD44low_C.kit._36

P3_AGM_VE.cad_High_CD44_low_04P3_AGM_VE.cad_High_CD44_low_04P5_AGM_VE.cad_High_CD44_low_14 P3_AGM_VE.cad_High_CD44_low_04P5_AGM_VE.cad_High_CD44_low_14P3_AGM_VE.cad_High_CD44_low_04P5_AGM_VE.cad_High_CD44_low_14 P3_AGM_VE.cad_High_CD44_low_15P6_CD44low_C.kit._16P6_CD44low_C.kit._16 SMAD3 P6_CD44low_C.kit._16P6_CD44low_C.kit._16 P1_AGM_VE.cad._CD44._17.1P1_AGM_VE.cad._CD44._17.1P3_AGM_VE.cad_High_CD44_low_15 P1_AGM_VE.cad._CD44._17.1P3_AGM_VE.cad_High_CD44_low_15P1_AGM_VE.cad._CD44._17.1P3_AGM_VE.cad_High_CD44_low_15

P5_AGM_VE.cad_High_CD44_low_26P6_CD44low_C.kit._37P6_CD44low_C.kit._37 P5_AGM_VE.cad_High_CD44_low_26P6_CD44low_C.kit._37 P3_AGM_VE.cad_High_CD44_low_07P3_AGM_VE.cad_High_CD44_low_07P5_AGM_VE.cad_High_CD44_low_26 BMPR2 P3_AGM_VE.cad_High_CD44_low_07P6_CD44low_C.kit._37P3_AGM_VE.cad_High_CD44_low_07P5_AGM_VE.cad_High_CD44_low_26

P3_AGM_VE.cad_High_CD44_low_43P5_AGM_VE.cad_High_CD44_low_31P5_AGM_VE.cad_High_CD44_low_31 P5_AGM_VE.cad_High_CD44_low_31P5_AGM_VE.cad_High_CD44_low_31

P6_CD44low_C.kit._15.1P6_CD44low_C.kit._15.1P3_AGM_VE.cad_High_CD44_low_43 P3_AGM_VE.cad_High_CD44_low_43P3_AGM_VE.cad_High_CD44_low_43 TAL1 P6_CD44low_C.kit._15.1P6_CD44low_C.kit._15.1 P5_AGM_VE.cad_High_CD44_low_24P6_CD44low_C.kit._10P6_CD44low_C.kit._10 P5_AGM_VE.cad_High_CD44_low_24P6_CD44low_C.kit._10 P5_AGM_VE.cad_High_CD44_low_09P5_AGM_VE.cad_High_CD44_low_09P5_AGM_VE.cad_High_CD44_low_24 P5_AGM_VE.cad_High_CD44_low_09P6_CD44low_C.kit._10.1P5_AGM_VE.cad_High_CD44_low_09P5_AGM_VE.cad_High_CD44_low_24

P6_CD44low_C.kit._17.1P6_CD44low_C.kit._23.1P6_CD44low_C.kit._23.1 P6_CD44low_C.kit._17.1P6_CD44low_C.kit._23.1 P6_CD44low_C.kit._44.1P6_CD44low_C.kit._44.1P6_CD44low_C.kit._17.1 SMAD2 P6_CD44low_C.kit._44.1P6_CD44low_C.kit._23.1P6_CD44low_C.kit._44.1P6_CD44low_C.kit._17.1 P5_AGM_VE.cad_High_CD44_low_42P6_CD44low_C.kit._23P6_CD44low_C.kit._23 P5_AGM_VE.cad_High_CD44_low_42P6_CD44low_C.kit._23

P6_CD44low_C.kit._09.1P6_CD44low_C.kit._09.1P5_AGM_VE.cad_High_CD44_low_42 P6_CD44low_C.kit._23P5_AGM_VE.cad_High_CD44_low_42 P6_CD44low_C.kit._31P6_CD44low_C.kit._31 SMAD5 P5_AGM_VE.cad_High_CD44_low_18P6_CD44low_C.kit._09.1P6_CD44low_C.kit._31P6_CD44low_C.kit._09.1

P3_AGM_VE.cad._CD44._14P5_AGM_VE.cad_High_CD44_low_18P5_AGM_VE.cad_High_CD44_low_18 P6_CD44low_C.kit._31P5_AGM_VE.cad_High_CD44_low_18

P3_AGM_VE.cad._CD44._02P3_AGM_VE.cad._CD44._02P3_AGM_VE.cad._CD44._14 P3_AGM_VE.cad._CD44._02P3_AGM_VE.cad._CD44._14P3_AGM_VE.cad._CD44._02P3_AGM_VE.cad._CD44._14 P6_CD44low_C.kit._33 SMAD1 P5_AGM_VE.cad_High_CD44_low_20P5_AGM_VE.cad_High_CD44_low_20P6_CD44low_C.kit._33 P5_AGM_VE.cad_High_CD44_low_20P6_CD44low_C.kit._33P5_AGM_VE.cad_High_CD44_low_20P6_CD44low_C.kit._33 P1_AGM_VE.cad._CD44._03P1_AGM_VE.cad._CD44._07P1_AGM_VE.cad._CD44._07 P1_AGM_VE.cad._CD44._07P1_AGM_VE.cad._CD44._07

P1_AGM_VE.cad._CD44._14.1P1_AGM_VE.cad._CD44._14.1P1_AGM_VE.cad._CD44._03 P1_AGM_VE.cad._CD44._03P1_AGM_VE.cad._CD44._03 P1_AGM_VE.cad._CD44._19.1P1_AGM_VE.cad._CD44._19.1 LMO2 P1_AGM_VE.cad._CD44._19.1P1_AGM_VE.cad._CD44._14.1P1_AGM_VE.cad._CD44._19.1P1_AGM_VE.cad._CD44._14.1 P5_AGM_VE.cad._CD44._27P1_AGM_VE.cad._CD44._18P1_AGM_VE.cad._CD44._18 P1_AGM_VE.cad._CD44._18P1_AGM_VE.cad._CD44._18

P1_AGM_VE.cad._CD44._13.1P1_AGM_VE.cad._CD44._13.1P5_AGM_VE.cad._CD44._27 P5_AGM_VE.cad._CD44._27P1_AGM_VE.cad._CD44._13.1P1_AGM_VE.cad._CD44._13.1P5_AGM_VE.cad._CD44._27 P1_AGM_VE.cad._CD44._10P1_AGM_VE.cad._CD44._12P1_AGM_VE.cad._CD44._12 SMAD4 P1_AGM_VE.cad._CD44._12.1P1_AGM_VE.cad._CD44._12 P5_AGM_VE.cad_High_CD44_low_41P5_AGM_VE.cad_High_CD44_low_41P1_AGM_VE.cad._CD44._10 P5_AGM_VE.cad_High_CD44_low_41P1_AGM_VE.cad._CD44._10P5_AGM_VE.cad_High_CD44_low_41P1_AGM_VE.cad._CD44._10

P5_AGM_VE.cad._CD44._43P5_AGM_VE.cad_High_CD44_low_03P5_AGM_VE.cad_High_CD44_low_03 P5_AGM_VE.cad_High_CD44_low_03P5_AGM_VE.cad_High_CD44_low_03 P5_AGM_VE.cad._CD44._37P5_AGM_VE.cad._CD44._37P5_AGM_VE.cad._CD44._43 FLI1 P5_AGM_VE.cad._CD44._37P5_AGM_VE.cad._CD44._43P5_AGM_VE.cad._CD44._37P5_AGM_VE.cad._CD44._43 P5_AGM_VE.cad_High_CD44_low_08P5_AGM_VE.cad._CD44._08P5_AGM_VE.cad._CD44._08 P5_AGM_VE.cad._CD44._08P5_AGM_VE.cad._CD44._08

P5_AGM_VE.cad._CD44._31P5_AGM_VE.cad._CD44._31P5_AGM_VE.cad_High_CD44_low_08 P5_AGM_VE.cad._CD44._31P5_AGM_VE.cad_High_CD44_low_08P5_AGM_VE.cad._CD44._31P5_AGM_VE.cad_High_CD44_low_08 P5_AGM_VE.cad_High_CD44_low_15P5_AGM_VE.cad._CD44._06P5_AGM_VE.cad._CD44._06 TGFBR1 P5_AGM_VE.cad._CD44._06P5_AGM_VE.cad._CD44._06 P3_AGM_VE.cad._CD44._41P3_AGM_VE.cad._CD44._41P5_AGM_VE.cad_High_CD44_low_15 P3_AGM_VE.cad._CD44._41P5_AGM_VE.cad_High_CD44_low_15P3_AGM_VE.cad._CD44._41P5_AGM_VE.cad_High_CD44_low_15

P5_AGM_VE.cad_High_CD44_low_30P5_AGM_VE.cad._CD44._38P5_AGM_VE.cad._CD44._38 P5_AGM_VE.cad._CD44._38P5_AGM_VE.cad._CD44._38 P5_AGM_VE.cad_High_CD44_low_06P5_AGM_VE.cad_High_CD44_low_06P5_AGM_VE.cad_High_CD44_low_30 NPR1 P5_AGM_VE.cad_High_CD44_low_06P5_AGM_VE.cad_High_CD44_low_30P5_AGM_VE.cad_High_CD44_low_06P5_AGM_VE.cad_High_CD44_low_30

P5_AGM_VE.cad._CD44._04P5_AGM_VE.cad_High_CD44_low_33P5_AGM_VE.cad_High_CD44_low_33 P5_AGM_VE.cad_High_CD44_low_33P5_AGM_VE.cad_High_CD44_low_33

P5_AGM_VE.cad_High_CD44_low_44P5_AGM_VE.cad_High_CD44_low_44P5_AGM_VE.cad._CD44._04 P5_AGM_VE.cad_High_CD44_low_44P5_AGM_VE.cad._CD44._04 MEIS2 P5_AGM_VE.cad._CD44._04P5_AGM_VE.cad_High_CD44_low_44 P1_AGM_VE.cad._CD44._22.1P5_AGM_VE.cad._CD44._39P5_AGM_VE.cad._CD44._39 P5_AGM_VE.cad._CD44._39P5_AGM_VE.cad._CD44._39

P1_AGM_VE.cad._CD44._01P1_AGM_VE.cad._CD44._22.1 Oatley, Vargel P1_AGM_VE.cad._CD44._22.1P1_AGM_VE.cad._CD44._22.1

P3_AGM_VE.cad._CD44._03P3_AGM_VE.cad._CD44._03P1_AGM_VE.cad._CD44._01 P3_AGM_VE.cad._CD44._03P1_AGM_VE.cad._CD44._01P3_AGM_VE.cad._CD44._03P1_AGM_VE.cad._CD44._01 P3_AGM_VE.cad_High_CD44_low_42 SOX7 P3_AGM_VE.cad._CD44._04P3_AGM_VE.cad._CD44._04P3_AGM_VE.cad_High_CD44_low_42 P3_AGM_VE.cad._CD44._04P3_AGM_VE.cad_High_CD44_low_42P3_AGM_VE.cad._CD44._04P3_AGM_VE.cad_High_CD44_low_42 P5_AGM_VE.cad_High_CD44_low_40P5_AGM_VE.cad._CD44._15P5_AGM_VE.cad._CD44._15 P5_AGM_VE.cad._CD44._15P5_AGM_VE.cad._CD44._15

P6_CD44low_C.kit._24.1P6_CD44low_C.kit._24.1P5_AGM_VE.cad_High_CD44_low_40 P5_AGM_VE.cad_High_CD44_low_40P5_AGM_VE.cad_High_CD44_low_40 P1_AGM_VE.cad._CD44._05.1P1_AGM_VE.cad._CD44._05.1 ERG P1_AGM_VE.cad._CD44._05.1P6_CD44low_C.kit._24.1P1_AGM_VE.cad._CD44._05.1P6_CD44low_C.kit._24.1

P5_AGM_VE.cad._CD44._32P1_AGM_VE.cad._CD44._21.1P1_AGM_VE.cad._CD44._21.1 P1_AGM_VE.cad._CD44._21P1_AGM_VE.cad._CD44._21.1

P5_AGM_VE.cad_High_CD44_low_38P5_AGM_VE.cad_High_CD44_low_38P5_AGM_VE.cad._CD44._32 P5_AGM_VE.cad_High_CD44_low_38P5_AGM_VE.cad._CD44._32P5_AGM_VE.cad_High_CD44_low_38P5_AGM_VE.cad._CD44._32 P5_AGM_VE.cad._CD44._05 DPYSL3 P5_AGM_VE.cad._CD44._02P5_AGM_VE.cad._CD44._02P5_AGM_VE.cad._CD44._05 P5_AGM_VE.cad._CD44._02P5_AGM_VE.cad._CD44._05P5_AGM_VE.cad._CD44._02P5_AGM_VE.cad._CD44._05 P5_AGM_VE.cad._CD44._03P5_AGM_VE.cad._CD44._01P5_AGM_VE.cad._CD44._01 P5_AGM_VE.cad._CD44._01P5_AGM_VE.cad._CD44._01

P5_AGM_VE.cad_High_CD44_low_43P5_AGM_VE.cad_High_CD44_low_43P5_AGM_VE.cad._CD44._03 P5_AGM_VE.cad_High_CD44_low_43P5_AGM_VE.cad._CD44._03 P1_AGM_VE.cad._CD44._21P1_AGM_VE.cad._CD44._21 SMAD6 P1_AGM_VE.cad._CD44._21.1P5_AGM_VE.cad._CD44._03P1_AGM_VE.cad._CD44._21P5_AGM_VE.cad_High_CD44_low_43 P6_CD44low_C.kit._32.1P3_AGM_VE.cad._CD44._01P3_AGM_VE.cad._CD44._01 P3_AGM_VE.cad._CD44._01P3_AGM_VE.cad._CD44._01

P6_CD44low_C.kit._13.1P6_CD44low_C.kit._13.1P6_CD44low_C.kit._32.1 P6_CD44low_C.kit._13.1P6_CD44low_C.kit._32P6_CD44low_C.kit._13.1P6_CD44low_C.kit._32.1 P1_AGM_VE.cad._CD44._11P3_AGM_VE.cad_High_CD44_low_39P3_AGM_VE.cad_High_CD44_low_39 FBN1 P1_AGM_VE.cad._CD44._11P3_AGM_VE.cad_High_CD44_low_39 P3_AGM_VE.cad._CD44._39P3_AGM_VE.cad._CD44._39P1_AGM_VE.cad._CD44._11 P3_AGM_VE.cad._CD44._21P3_AGM_VE.cad_High_CD44_low_39P3_AGM_VE.cad._CD44._39P1_AGM_VE.cad._CD44._11

P3_AGM_VE.cad._CD44._44P3_AGM_VE.cad._CD44._21P3_AGM_VE.cad._CD44._21 P3_AGM_VE.cad._CD44._39P3_AGM_VE.cad._CD44._21 P5_AGM_VE.cad._CD44._09P5_AGM_VE.cad._CD44._09P3_AGM_VE.cad._CD44._44 SMAD7 P5_AGM_VE.cad_High_CD44_low_27P3_AGM_VE.cad._CD44._44P5_AGM_VE.cad._CD44._09P3_AGM_VE.cad._CD44._44 P5_AGM_VE.cad._CD44._29P5_AGM_VE.cad_High_CD44_low_27P5_AGM_VE.cad_High_CD44_low_27 P5_AGM_VE.cad._CD44._09P5_AGM_VE.cad_High_CD44_low_27

P5_AGM_VE.cad_High_CD44_low_05P5_AGM_VE.cad_High_CD44_low_05P5_AGM_VE.cad._CD44._29 P5_AGM_VE.cad._CD44._29P5_AGM_VE.cad_High_CD44_low_05P5_AGM_VE.cad_High_CD44_low_05P5_AGM_VE.cad._CD44._29 P3_AGM_VE.cad_High_CD44_low_19P3_AGM_VE.cad_High_CD44_low_36P3_AGM_VE.cad_High_CD44_low_36 LYL1 P3_AGM_VE.cad_High_CD44_low_36P3_AGM_VE.cad_High_CD44_low_36 P3_AGM_VE.cad._CD44._07P3_AGM_VE.cad._CD44._07P3_AGM_VE.cad_High_CD44_low_19 P3_AGM_VE.cad._CD44._07P3_AGM_VE.cad_High_CD44_low_19P3_AGM_VE.cad._CD44._07P3_AGM_VE.cad_High_CD44_low_19

P3_AGM_VE.cad_High_CD44_low_23P3_AGM_VE.cad._CD44._26P3_AGM_VE.cad._CD44._26 P3_AGM_VE.cad._CD44._26P3_AGM_VE.cad._CD44._26 P1_AGM_VE.cad._CD44._20P1_AGM_VE.cad._CD44._20P3_AGM_VE.cad_High_CD44_low_23 ACVR1B P1_AGM_VE.cad._CD44._17P3_AGM_VE.cad_High_CD44_low_23P1_AGM_VE.cad._CD44._20P3_AGM_VE.cad_High_CD44_low_23

P1_AGM_VE.cad._CD44._09.1P1_AGM_VE.cad._CD44._17P1_AGM_VE.cad._CD44._17 P1_AGM_VE.cad._CD44._20.1P1_AGM_VE.cad._CD44._17

P3_AGM_VE.cad._CD44._38P3_AGM_VE.cad._CD44._38P1_AGM_VE.cad._CD44._09.1 E11.5) P1_AGM_VE.cad._CD44._09.1P1_AGM_VE.cad._CD44._09.1 P3_AGM_VE.cad._CD44._23P3_AGM_VE.cad._CD44._23 BMPR1A P3_AGM_VE.cad._CD44._23P3_AGM_VE.cad._CD44._38

- P3_AGM_VE.cad._CD44._38P3_AGM_VE.cad._CD44._23

P5_AGM_VE.cad._CD44._28P3_AGM_VE.cad._CD44._36P3_AGM_VE.cad._CD44._36 P3_AGM_VE.cad._CD44._36P3_AGM_VE.cad._CD44._36

P1_AGM_VE.cad._CD44._16P1_AGM_VE.cad._CD44._16P5_AGM_VE.cad._CD44._28 P1_AGM_VE.cad._CD44._16.1P5_AGM_VE.cad._CD44._28P1_AGM_VE.cad._CD44._16P5_AGM_VE.cad._CD44._28 P5_AGM_VE.cad._CD44._34 PALLD P5_AGM_VE.cad._CD44._12P5_AGM_VE.cad._CD44._12P5_AGM_VE.cad._CD44._34 P5_AGM_VE.cad._CD44._34P5_AGM_VE.cad._CD44._12P5_AGM_VE.cad._CD44._12P5_AGM_VE.cad._CD44._34 P5_AGM_VE.cad._CD44._36P5_AGM_VE.cad._CD44._10P5_AGM_VE.cad._CD44._10 P5_AGM_VE.cad._CD44._10P5_AGM_VE.cad._CD44._10

P5_AGM_VE.cad_High_CD44_low_46P5_AGM_VE.cad_High_CD44_low_46P5_AGM_VE.cad._CD44._36 P5_AGM_VE.cad_High_CD44_low_46P5_AGM_VE.cad._CD44._36 P6_CD44low_C.kit._45P6_CD44low_C.kit._45 PECAM1 P6_CD44low_C.kit._45P5_AGM_VE.cad._CD44._36P6_CD44low_C.kit._45P5_AGM_VE.cad_High_CD44_low_46

P5_AGM_VE.cad_High_CD44_low_19P5_AGM_VE.cad_High_CD44_low_36P5_AGM_VE.cad_High_CD44_low_36 P5_AGM_VE.cad_High_CD44_low_36P5_AGM_VE.cad_High_CD44_low_36

P5_AGM_VE.cad_High_CD44_low_02P5_AGM_VE.cad_High_CD44_low_02P5_AGM_VE.cad_High_CD44_low_19 P5_AGM_VE.cad_High_CD44_low_19P5_AGM_VE.cad_High_CD44_low_02P5_AGM_VE.cad_High_CD44_low_02P5_AGM_VE.cad_High_CD44_low_19 P5_AGM_VE.cad._CD44._20 ENG P5_AGM_VE.cad._CD44._11P5_AGM_VE.cad._CD44._11P5_AGM_VE.cad._CD44._20 P5_AGM_VE.cad._CD44._11P5_AGM_VE.cad._CD44._20P5_AGM_VE.cad._CD44._11P5_AGM_VE.cad._CD44._20 P1_AGM_VE.cad._CD44._05P1_AGM_VE.cad._CD44._13P1_AGM_VE.cad._CD44._13 P1_AGM_VE.cad._CD44._05P1_AGM_VE.cad._CD44._13

P3_AGM_VE.cad._CD44._15P3_AGM_VE.cad._CD44._15P1_AGM_VE.cad._CD44._05 P1_AGM_VE.cad._CD44._13P1_AGM_VE.cad._CD44._05 P1_AGM_VE.cad._CD44._09P1_AGM_VE.cad._CD44._09 ACVRL1 P1_AGM_VE.cad._CD44._09P3_AGM_VE.cad._CD44._15P1_AGM_VE.cad._CD44._09P3_AGM_VE.cad._CD44._15 P3_AGM_VE.cad._CD44._06P3_AGM_VE.cad._CD44._46P3_AGM_VE.cad._CD44._46 P3_AGM_VE.cad._CD44._06P3_AGM_VE.cad._CD44._46

P5_AGM_VE.cad._CD44._07P5_AGM_VE.cad._CD44._07P3_AGM_VE.cad._CD44._06 P5_AGM_VE.cad._CD44._07P3_AGM_VE.cad._CD44._46P5_AGM_VE.cad._CD44._07P3_AGM_VE.cad._CD44._06 P1_AGM_VE.cad._CD44._22P5_AGM_VE.cad._CD44._30P5_AGM_VE.cad._CD44._30 TEK P5_AGM_VE.cad._CD44._30P5_AGM_VE.cad._CD44._30 P3_AGM_VE.cad._CD44._28P3_AGM_VE.cad._CD44._28P1_AGM_VE.cad._CD44._22 P3_AGM_VE.cad._CD44._28P1_AGM_VE.cad._CD44._22P3_AGM_VE.cad._CD44._28P1_AGM_VE.cad._CD44._22

P3_AGM_VE.cad._CD44._22P3_AGM_VE.cad._CD44._27P3_AGM_VE.cad._CD44._27 P3_AGM_VE.cad._CD44._27P3_AGM_VE.cad._CD44._27 P3_AGM_VE.cad._CD44._42P3_AGM_VE.cad._CD44._42P3_AGM_VE.cad._CD44._22 COL4A2 P3_AGM_VE.cad._CD44._42P3_AGM_VE.cad._CD44._22P3_AGM_VE.cad._CD44._42P3_AGM_VE.cad._CD44._22 P3_AGM_VE.cad._CD44._35P1_AGM_VE.cad._CD44._02P1_AGM_VE.cad._CD44._02 P1_AGM_VE.cad._CD44._02P1_AGM_VE.cad._CD44._02

P3_AGM_VE.cad._CD44._20P3_AGM_VE.cad._CD44._20P3_AGM_VE.cad._CD44._35 P3_AGM_VE.cad._CD44._20P3_AGM_VE.cad._CD44._35P3_AGM_VE.cad._CD44._20P3_AGM_VE.cad._CD44._35 P3_AGM_VE.cad_High_CD44_low_22P3_AGM_VE.cad._CD44._13P3_AGM_VE.cad._CD44._13 PTPRB P3_AGM_VE.cad._CD44._13P3_AGM_VE.cad._CD44._13 P1_AGM_VE.cad._CD44._19P1_AGM_VE.cad._CD44._19P3_AGM_VE.cad_High_CD44_low_22 P3_AGM_VE.cad_High_CD44_low_22P1_AGM_VE.cad._CD44._19P1_AGM_VE.cad._CD44._19P3_AGM_VE.cad_High_CD44_low_22

P1_AGM_VE.cad._CD44._23P5_AGM_VE.cad_High_CD44_low_45P5_AGM_VE.cad_High_CD44_low_45 P5_AGM_VE.cad_High_CD44_low_45P5_AGM_VE.cad_High_CD44_low_45 P5_AGM_VE.cad._CD44._16P5_AGM_VE.cad._CD44._16P1_AGM_VE.cad._CD44._23 PTPRM P5_AGM_VE.cad_High_CD44_low_22P1_AGM_VE.cad._CD44._23P5_AGM_VE.cad._CD44._16P1_AGM_VE.cad._CD44._23 P1_AGM_VE.cad._CD44._20.1P5_AGM_VE.cad_High_CD44_low_22P5_AGM_VE.cad_High_CD44_low_22 P5_AGM_VE.cad._CD44._16P5_AGM_VE.cad_High_CD44_low_22

P5_AGM_VE.cad._CD44._44P5_AGM_VE.cad._CD44._44P1_AGM_VE.cad._CD44._20.1 P1_AGM_VE.cad._CD44._20P1_AGM_VE.cad._CD44._20.1 P5_AGM_VE.cad._CD44._21P5_AGM_VE.cad._CD44._21 RAMP2 P5_AGM_VE.cad._CD44._21P5_AGM_VE.cad._CD44._44P5_AGM_VE.cad._CD44._21P5_AGM_VE.cad._CD44._44

P5_AGM_VE.cad._CD44._41P5_AGM_VE.cad._CD44._40P5_AGM_VE.cad._CD44._40 P5_AGM_VE.cad._CD44._40P5_AGM_VE.cad._CD44._40

P5_AGM_VE.cad._CD44._23P5_AGM_VE.cad._CD44._23P5_AGM_VE.cad._CD44._41 P5_AGM_VE.cad._CD44._41P5_AGM_VE.cad._CD44._23P5_AGM_VE.cad._CD44._23P5_AGM_VE.cad._CD44._41 P5_AGM_VE.cad_High_CD44_low_23 KDR P5_AGM_VE.cad._CD44._18P5_AGM_VE.cad._CD44._18P5_AGM_VE.cad_High_CD44_low_23 P5_AGM_VE.cad_High_CD44_low_23P5_AGM_VE.cad_High_CD44_low_23 PCR analysis of the four populations populations four the of analysis PCR P5_AGM_VE.cad._CD44._18P5_AGM_VE.cad._CD44._18

P5_AGM_VE.cad._CD44._25P5_AGM_VE.cad._CD44._26P5_AGM_VE.cad._CD44._26 P5_AGM_VE.cad._CD44._25P5_AGM_VE.cad._CD44._26

P5_AGM_VE.cad._CD44._24P5_AGM_VE.cad._CD44._24P5_AGM_VE.cad._CD44._25 P5_AGM_VE.cad._CD44._26P5_AGM_VE.cad._CD44._25 P5_AGM_VE.cad._CD44._22P5_AGM_VE.cad._CD44._22 CDH5 P5_AGM_VE.cad._CD44._24P5_AGM_VE.cad._CD44._24

- P5_AGM_VE.cad._CD44._22P5_AGM_VE.cad._CD44._22

P6_CD44low_C.kit._13P6_CD44low_C.kit._26P6_CD44low_C.kit._26 P6_CD44low_C.kit._26P6_CD44low_C.kit._26

P6_CD44low_C.kit._25.1P6_CD44low_C.kit._25.1P6_CD44low_C.kit._13 P6_CD44low_C.kit._25P6_CD44low_C.kit._13P6_CD44low_C.kit._25.1P6_CD44low_C.kit._13 P5_AGM_VE.cad_High_CD44_low_07 TGFB1 P3_AGM_VE.cad_High_CD44_low_06P3_AGM_VE.cad_High_CD44_low_06P5_AGM_VE.cad_High_CD44_low_07 P3_AGM_VE.cad_High_CD44_low_06P5_AGM_VE.cad_High_CD44_low_07P3_AGM_VE.cad_High_CD44_low_06P5_AGM_VE.cad_High_CD44_low_07 P6_CD44low_C.kit._03P6_CD44low_C.kit._41P6_CD44low_C.kit._41 P6_CD44low_C.kit._03P6_CD44low_C.kit._41

P3_AGM_VE.cad_High_CD44_low_30P3_AGM_VE.cad_High_CD44_low_30P6_CD44low_C.kit._03 P6_CD44low_C.kit._41P6_CD44low_C.kit._03 P3_AGM_VE.cad_High_CD44_low_20P3_AGM_VE.cad_High_CD44_low_20 CTNNB1 P3_AGM_VE.cad_High_CD44_low_20P3_AGM_VE.cad_High_CD44_low_30P3_AGM_VE.cad_High_CD44_low_20P3_AGM_VE.cad_High_CD44_low_30 P3_AGM_VE.cad._CD44._31P5_AGM_VE.cad_High_CD44_low_10P5_AGM_VE.cad_High_CD44_low_10 P5_AGM_VE.cad_High_CD44_low_10P5_AGM_VE.cad_High_CD44_low_10

P3_AGM_VE.cad_High_CD44_low_45P3_AGM_VE.cad_High_CD44_low_45P3_AGM_VE.cad._CD44._31 P3_AGM_VE.cad_High_CD44_low_45P3_AGM_VE.cad._CD44._31P3_AGM_VE.cad_High_CD44_low_45P3_AGM_VE.cad._CD44._31 P1_AGM_VE.cad._CD44._15P1_AGM_VE.cad._CD44._15 PPIA P1_AGM_VE.cad._CD44._15P1_AGM_VE.cad._CD44._15 P1_AGM_VE.cad._CD44._16.1P1_AGM_VE.cad._CD44._04P1_AGM_VE.cad._CD44._04 P1_AGM_VE.cad._CD44._04P1_AGM_VE.cad._CD44._04

P1_AGM_VE.cad._CD44._14P1_AGM_VE.cad._CD44._14P1_AGM_VE.cad._CD44._16.1 P1_AGM_VE.cad._CD44._14P1_AGM_VE.cad._CD44._16.1

RT P6_CD44low_C.kit._34 P1_AGM_VE.cad._CD44._16P1_AGM_VE.cad._CD44._14 P6_CD44low_C.kit._34 SFPI1 P6_CD44low_C.kit._09P6_CD44low_C.kit._34 P6_CD44low_C.kit._09 (E9.5) pairs somite 26 (E10) pairs somite 32 (E10.5) pairs somite 39 (E11 pairs somite 45 P6_CD44low_C.kit._15P6_CD44low_C.kit._15P6_CD44low_C.kit._09 P6_CD44low_C.kit._15P6_CD44low_C.kit._34P6_CD44low_C.kit._15P6_CD44low_C.kit._09

P6_CD44low_C.kit._01P6_CD44low_C.kit._01- - - -

- P3_AGM_VE.cad_High_CD44_low_46P3_AGM_VE.cad_High_CD44_low_46 P3_AGM_VE.cad_High_CD44_low_46P6_CD44low_C.kit._01P3_AGM_VE.cad_High_CD44_low_46P6_CD44low_C.kit._01 P6_CD44low_C.kit._38P3_AGM_VE.cad_High_CD44_low_34P3_AGM_VE.cad_High_CD44_low_34 RUNX1 P3_AGM_VE.cad_High_CD44_low_34P3_AGM_VE.cad_High_CD44_low_34

P6_CD44low_C.kit._18P6_CD44low_C.kit._18P6_CD44low_C.kit._38 P6_CD44low_C.kit._38P6_CD44low_C.kit._18P6_CD44low_C.kit._18P6_CD44low_C.kit._38

P6_CD44low_C.kit._25P3_AGM_VE.cad_High_CD44_low_21P3_AGM_VE.cad_High_CD44_low_21 P3_AGM_VE.cad_High_CD44_low_21P3_AGM_VE.cad_High_CD44_low_21 P6_CD44low_C.kit._25 PTPRC P6_CD44low_C.kit._24P6_CD44low_C.kit._25 P6_CD44low_C.kit._11.1P6_CD44low_C.kit._24P6_CD44low_C.kit._24 P6_CD44low_C.kit._25.1P6_CD44low_C.kit._24 P5_AGM_VE.cad_High_CD44_low_34P6_CD44low_C.kit._11.123 30 36 42 P6_CD44low_C.kit._11P6_CD44low_C.kit._11.1

P3_AGM_VE.cad_High_CD44_low_29P3_AGM_VE.cad_High_CD44_low_29P5_AGM_VE.cad_High_CD44_low_34 P5_AGM_VE.cad_High_CD44_low_34P5_AGM_VE.cad_High_CD44_low_34 P3_AGM_VE.cad_High_CD44_low_05P3_AGM_VE.cad_High_CD44_low_05 EPS8 P3_AGM_VE.cad_High_CD44_low_05P3_AGM_VE.cad_High_CD44_low_29P3_AGM_VE.cad_High_CD44_low_05P3_AGM_VE.cad_High_CD44_low_29

P3_AGM_VE.cad_High_CD44_low_10P3_AGM_VE.cad_High_CD44_low_03P3_AGM_VE.cad_High_CD44_low_03 P3_AGM_VE.cad_High_CD44_low_03P3_AGM_VE.cad_High_CD44_low_03

P3_AGM_VE.cad_High_CD44_low_33P3_AGM_VE.cad_High_CD44_low_33P3_AGM_VE.cad_High_CD44_low_10 P3_AGM_VE.cad_High_CD44_low_33P3_AGM_VE.cad_High_CD44_low_10P3_AGM_VE.cad_High_CD44_low_33P3_AGM_VE.cad_High_CD44_low_10 P3_AGM_VE.cad_High_CD44_low_13 ENPP1 P6_CD44low_C.kit._39P6_CD44low_C.kit._39P3_AGM_VE.cad_High_CD44_low_13 P6_CD44low_C.kit._04P3_AGM_VE.cad_High_CD44_low_13P6_CD44low_C.kit._39P3_AGM_VE.cad_High_CD44_low_13 P6_CD44low_C.kit._35P6_CD44low_C.kit._04P6_CD44low_C.kit._04 P6_CD44low_C.kit._39P6_CD44low_C.kit._04

P3_AGM_VE.cad_High_CD44_low_44P3_AGM_VE.cad_High_CD44_low_44P6_CD44low_C.kit._35 P6_CD44low_C.kit._35P6_CD44low_C.kit._35 P3_AGM_VE.cad_High_CD44_low_14P3_AGM_VE.cad_High_CD44_low_14 SLA P3_AGM_VE.cad_High_CD44_low_14P3_AGM_VE.cad_High_CD44_low_44P3_AGM_VE.cad_High_CD44_low_14P3_AGM_VE.cad_High_CD44_low_44

P3_AGM_VE.cad_High_CD44_low_38P3_AGM_VE.cad_High_CD44_low_17P3_AGM_VE.cad_High_CD44_low_17 P3_AGM_VE.cad_High_CD44_low_17P3_AGM_VE.cad_High_CD44_low_17

P3_AGM_VE.cad_High_CD44_low_12P3_AGM_VE.cad_High_CD44_low_12P3_AGM_VE.cad_High_CD44_low_38stages Embryo P3_AGM_VE.cad_High_CD44_low_12P3_AGM_VE.cad_High_CD44_low_38P3_AGM_VE.cad_High_CD44_low_12P3_AGM_VE.cad_High_CD44_low_38 P1_AGM_VE.cad._CD44._12.1 ITGAM P3_AGM_VE.cad_High_CD44_low_25P3_AGM_VE.cad_High_CD44_low_25P1_AGM_VE.cad._CD44._12.1 P3_AGM_VE.cad_High_CD44_low_25P1_AGM_VE.cad._CD44._12P3_AGM_VE.cad_High_CD44_low_25P1_AGM_VE.cad._CD44._12.1 P6_CD44low_C.kit._29P6_CD44low_C.kit._30P6_CD44low_C.kit._30 P6_CD44low_C.kit._29P6_CD44low_C.kit._30

P6_CD44low_C.kit._40P6_CD44low_C.kit._40P6_CD44low_C.kit._29 P6_CD44low_C.kit._30P6_CD44low_C.kit._29 P6_CD44low_C.kit._22P6_CD44low_C.kit._22 GPR126 P6_CD44low_C.kit._22P6_CD44low_C.kit._40P6_CD44low_C.kit._22P6_CD44low_C.kit._40 cell q cell P6_CD44low_C.kit._20P6_CD44low_C.kit._46P6_CD44low_C.kit._46 P6_CD44low_C.kit._46P6_CD44low_C.kit._46

P5_AGM_VE.cad_High_CD44_low_37P5_AGM_VE.cad_High_CD44_low_37P6_CD44low_C.kit._20 P5_AGM_VE.cad_High_CD44_low_11P6_CD44low_C.kit._20P5_AGM_VE.cad_High_CD44_low_37P6_CD44low_C.kit._20 P5_AGM_VE.cad_High_CD44_low_11P5_AGM_VE.cad_High_CD44_low_11 COL4A5 P5_AGM_VE.cad_High_CD44_low_37P5_AGM_VE.cad_High_CD44_low_11 - P6_CD44low_C.kit._43 P5_AGM_VE.cad_High_CD44_low_29P5_AGM_VE.cad_High_CD44_low_29P6_CD44low_C.kit._43 P6_CD44low_C.kit._43.1P5_AGM_VE.cad_High_CD44_low_29P5_AGM_VE.cad_High_CD44_low_29P6_CD44low_C.kit._43

P6_CD44low_C.kit._38.1P6_CD44low_C.kit._17P6_CD44low_C.kit._17 P6_CD44low_C.kit._17P6_CD44low_C.kit._17 P6_CD44low_C.kit._42P6_CD44low_C.kit._42P6_CD44low_C.kit._38.1 ACTA2 P6_CD44low_C.kit._38.1P6_CD44low_C.kit._42P6_CD44low_C.kit._42P6_CD44low_C.kit._38.1

P5_AGM_VE.cad_High_CD44_low_16P5_AGM_VE.cad_High_CD44_low_16 Pos P6_CD44low_C.kit._12 Neg P5_AGM_VE.cad_High_CD44_low_16P5_AGM_VE.cad_High_CD44_low_16

P6_CD44low_C.kit._06P6_CD44low_C.kit._06P6_CD44low_C.kit._12 P6_CD44low_C.kit._12P6_CD44low_C.kit._06P6_CD44low_C.kit._06P6_CD44low_C.kit._12 P5_AGM_VE.cad_High_CD44_low_17P6_CD44low_C.kit._21P6_CD44low_C.kit._21 SNAI2 P5_AGM_VE.cad_High_CD44_low_17P6_CD44low_C.kit._21 P6_CD44low_C.kit._05P6_CD44low_C.kit._05P5_AGM_VE.cad_High_CD44_low_17 P6_CD44low_C.kit._05P6_CD44low_C.kit._21P6_CD44low_C.kit._05P5_AGM_VE.cad_High_CD44_low_17

P6_CD44low_C.kit._44P6_CD44low_C.kit._39.1P6_CD44low_C.kit._39.1 P6_CD44low_C.kit._39.1P6_CD44low_C.kit._39.1 P6_CD44low_C.kit._02P6_CD44low_C.kit._02P6_CD44low_C.kit._44 SASH1 P6_CD44low_C.kit._44P6_CD44low_C.kit._44 Kit P6_CD44low_C.kit._02P6_CD44low_C.kit._02

P5_AGM_VE.cad_High_CD44_low_12P6_CD44low_C.kit._27P6_CD44low_C.kit._27 Kit P5_AGM_VE.cad_High_CD44_low_12P6_CD44low_C.kit._27

P6_CD44low_C.kit._10.1P6_CD44low_C.kit._10.1P5_AGM_VE.cad_High_CD44_low_12 P6_CD44low_C.kit._27P5_AGM_VE.cad_High_CD44_low_12 BH1 − P5_AGM_VE.cad_High_CD44_low_39P5_AGM_VE.cad_High_CD44_low_39 HBB P5_AGM_VE.cad_High_CD44_low_39P6_CD44low_C.kit._10P5_AGM_VE.cad_High_CD44_low_39P6_CD44low_C.kit._10.1

P6_CD44low_C.kit._18.1P6_CD44low_C.kit._43.1P6_CD44low_C.kit._43.1 P6_CD44low_C.kit._43P6_CD44low_C.kit._43.1

P6_CD44low_C.kit._34.1P6_CD44low_C.kit._34.1P6_CD44low_C.kit._18.1 P6_CD44low_C.kit._34.1P6_CD44low_C.kit._18.1P6_CD44low_C.kit._34.1P6_CD44low_C.kit._18.1 P6_CD44low_C.kit._32 FLRT2 P6_CD44low_C.kit._33.1P6_CD44low_C.kit._33.1P6_CD44low_C.kit._32 P6_CD44low_C.kit._32.1P6_CD44low_C.kit._32 Low High P6_CD44low_C.kit._33.1P6_CD44low_C.kit._33.1 Low P6_CD44low_C.kit._11P6_CD44low_C.kit._30.1P6_CD44low_C.kit._30.1Neg P6_CD44low_C.kit._11.1P6_CD44low_C.kit._30.1

P6_CD44low_C.kit._27.1P6_CD44low_C.kit._27.1P6_CD44low_C.kit._11 P6_CD44low_C.kit._30.1P6_CD44low_C.kit._11 P6_CD44low_C.kit._20.1P6_CD44low_C.kit._20.1 CACNA2D1 P6_CD44low_C.kit._27.1P6_CD44low_C.kit._20.1P6_CD44low_C.kit._20.1P6_CD44low_C.kit._27.1

P6_CD44low_C.kit._08P5_AGM_VE.cad_High_CD44_low_32P5_AGM_VE.cad_High_CD44_low_32 P5_AGM_VE.cad_High_CD44_low_32P5_AGM_VE.cad_High_CD44_low_32

P6_CD44low_C.kit._46.1P6_CD44low_C.kit._46.1P6_CD44low_C.kit._08 P6_CD44low_C.kit._46.1P6_CD44low_C.kit._08.1P6_CD44low_C.kit._46.1P6_CD44low_C.kit._08 P6_CD44low_C.kit._05.1 CLDN5 P5_AGM_VE.cad_High_CD44_low_13P5_AGM_VE.cad_High_CD44_low_13P6_CD44low_C.kit._05.1 P5_AGM_VE.cad_High_CD44_low_13P6_CD44low_C.kit._05.1P5_AGM_VE.cad_High_CD44_low_13P6_CD44low_C.kit._05.1

P6_CD44low_C.kit._36.1P3_AGM_VE.cad_High_CD44_low_18P3_AGM_VE.cad_High_CD44_low_18 P3_AGM_VE.cad_High_CD44_low_18P3_AGM_VE.cad_High_CD44_low_18

P6_CD44low_C.kit._03.1P6_CD44low_C.kit._03.1P6_CD44low_C.kit._36.1 P6_CD44low_C.kit._03.1P6_CD44low_C.kit._36.1 TGFB3 P6_CD44low_C.kit._36.1P6_CD44low_C.kit._03.1 Single P6_CD44low_C.kit._14P6_CD44low_C.kit._14 P6_CD44low_C.kit._14P6_CD44low_C.kit._14

P6_CD44low_C.kit._06.1P6_CD44low_C.kit._42.1P6_CD44low_C.kit._42.1 P6_CD44low_C.kit._06.1P6_CD44low_C.kit._42.1

P6_CD44low_C.kit._19P6_CD44low_C.kit._19P6_CD44low_C.kit._06.1 P6_CD44low_C.kit._19P6_CD44low_C.kit._42.1P6_CD44low_C.kit._19P6_CD44low_C.kit._06.1 P5_AGM_VE.cad_High_CD44_low_28 GRIA4 P6_CD44low_C.kit._41.1P6_CD44low_C.kit._41.1P5_AGM_VE.cad_High_CD44_low_28 P5_AGM_VE.cad_High_CD44_low_28P5_AGM_VE.cad_High_CD44_low_28

CD44 P6_CD44low_C.kit._12.1P6_CD44low_C.kit._41.1 P6_CD44low_C.kit._12.1P6_CD44low_C.kit._12.1 CD44 CD44 CD44 P6_CD44low_C.kit._41.1P6_CD44low_C.kit._12.1

P6_CD44low_C.kit._01.1P6_CD44low_C.kit._26.1P6_CD44low_C.kit._26.1 P6_CD44low_C.kit._26.1P6_CD44low_C.kit._26.1 P6_CD44low_C.kit._28P6_CD44low_C.kit._28P6_CD44low_C.kit._01.1 SMAD9 P6_CD44low_C.kit._01.1P6_CD44low_C.kit._28.1P6_CD44low_C.kit._28P6_CD44low_C.kit._01.1 P6_CD44low_C.kit._16.1P6_CD44low_C.kit._35.1P6_CD44low_C.kit._16.1P6_CD44low_C.kit._35.1 P6_CD44low_C.kit._16.1P6_CD44low_C.kit._35.1P6_CD44low_C.kit._16.1P6_CD44low_C.kit._35.1

and Kitand P6_CD44low_C.kit._45.1P6_CD44low_C.kit._45.1 P6_CD44low_C.kit._45.1P6_CD44low_C.kit._45.1 P6_CD44low_C.kit._40.1P3_AGM_VE.cad_High_CD44_low_11P3_AGM_VE.cad_High_CD44_low_11 LGR5 P3_AGM_VE.cad_High_CD44_low_11P3_AGM_VE.cad_High_CD44_low_11 P6_CD44low_C.kit._02.1P6_CD44low_C.kit._02.1P6_CD44low_C.kit._40.1 P6_CD44low_C.kit._02.1P6_CD44low_C.kit._40.1P6_CD44low_C.kit._02.1P6_CD44low_C.kit._40.1

P6_CD44low_C.kit._29.1P6_CD44low_C.kit._04.1P6_CD44low_C.kit._04.1 P6_CD44low_C.kit._04.1P6_CD44low_C.kit._04.1 P6_CD44low_C.kit._29.1 THPO P6_CD44low_C.kit._07.1P6_CD44low_C.kit._29.1 P6_CD44low_C.kit._28.1P6_CD44low_C.kit._07.1P6_CD44low_C.kit._07.1 P6_CD44low_C.kit._29.1P6_CD44low_C.kit._07.1

P6_CD44low_C.kit._21.1P6_CD44low_C.kit._21.1P6_CD44low_C.kit._28.1 P6_CD44low_C.kit._28P6_CD44low_C.kit._21.1P6_CD44low_C.kit._21.1P6_CD44low_C.kit._28.1 P6_CD44low_C.kit._22.1P6_CD44low_C.kit._22.1Groups EPO P6_CD44low_C.kit._08P6_CD44low_C.kit._22.1 P6_CD44low_C.kit._37.1P6_CD44low_C.kit._08.1P6_CD44low_C.kit._37.1P6_CD44low_C.kit._08.1 P6_CD44low_C.kit._37.1P6_CD44low_C.kit._22.1P6_CD44low_C.kit._37.1P6_CD44low_C.kit._08.1

P4_AGM_VE.cad_Low_CD44._46P6_CD44low_C.kit._31.1P4_AGM_VE.cad_Low_CD44._46P6_CD44low_C.kit._31.1 P4_AGM_VE.cad_Low_CD44._18P6_CD44low_C.kit._31.1P4_AGM_VE.cad_Low_CD44._46P6_CD44low_C.kit._31.1

P4_AGM_VE.cad_Low_CD44._18P4_AGM_VE.cad_Low_CD44._18 GATA1 P4_AGM_VE.cad_Low_CD44._46P4_AGM_VE.cad_Low_CD44._18

P4_AGM_VE.cad_Low_CD44._03P4_AGM_VE.cad_High_CD44_High_34P4_AGM_VE.cad_Low_CD44._03P4_AGM_VE.cad_High_CD44_High_34 P4_AGM_VE.cad_High_CD44_High_34P4_AGM_VE.cad_High_CD44_High_34

0 P4_AGM_VE.cad_Low_CD44._03P4_AGM_VE.cad_Low_CD44._03

P4_AGM_VE.cad_High_CD44_High_14P4_AGM_VE.cad_Low_CD44._23P4_AGM_VE.cad_High_CD44_High_14P4_AGM_VE.cad_Low_CD44._23 P4_AGM_VE.cad_Low_CD44._23P4_AGM_VE.cad_Low_CD44._23 0 GFI1B P4_AGM_VE.cad_High_CD44_High_14P4_AGM_VE.cad_High_CD44_High_14 P4_AGM_VE.cad_Low_CD44._22P4_AGM_VE.cad_Low_CD44._12P4_AGM_VE.cad_Low_CD44._22P4_AGM_VE.cad_Low_CD44._12 P4_AGM_VE.cad_Low_CD44._22P4_AGM_VE.cad_Low_CD44._12P4_AGM_VE.cad_Low_CD44._22P4_AGM_VE.cad_Low_CD44._12

P4_AGM_VE.cad_High_CD44_High_40P4_AGM_VE.cad_High_CD44_High_32P4_AGM_VE.cad_High_CD44_High_40P4_AGM_VE.cad_High_CD44_High_32 P4_AGM_VE.cad_High_CD44_High_28P4_AGM_VE.cad_High_CD44_High_32P4_AGM_VE.cad_High_CD44_High_40P4_AGM_VE.cad_High_CD44_High_32 P4_AGM_VE.cad_High_CD44_High_28P4_AGM_VE.cad_High_CD44_High_28 MPO P4_AGM_VE.cad_High_CD44_High_40P4_AGM_VE.cad_High_CD44_High_28 P4_AGM_VE.cad_Low_CD44._04P4_AGM_VE.cad_High_CD44_High_01P4_AGM_VE.cad_High_CD44_High_01 P4_AGM_VE.cad_High_CD44_High_01P4_AGM_VE.cad_High_CD44_High_01

Cad P4_AGM_VE.cad_Low_CD44._04 P4_AGM_VE.cad_High_CD44_High_43P4_AGM_VE.cad_Low_CD44._04

P4_AGM_VE.cad_High_CD44_High_17P4_AGM_VE.cad_High_CD44_High_43P4_AGM_VE.cad_High_CD44_High_17P4_AGM_VE.cad_High_CD44_High_43 P4_AGM_VE.cad_Low_CD44._04P4_AGM_VE.cad_High_CD44_High_43 EPOR P4_AGM_VE.cad_High_CD44_High_17P4_AGM_VE.cad_High_CD44_High_17 P4_AGM_VE.cad_Low_CD44._17P4_AGM_VE.cad_Low_CD44._19P4_AGM_VE.cad_Low_CD44._17P4_AGM_VE.cad_Low_CD44._19 P4_AGM_VE.cad_Low_CD44._17P4_AGM_VE.cad_Low_CD44._19 - P4_AGM_VE.cad_Low_CD44._19P4_AGM_VE.cad_Low_CD44._17

P4_AGM_VE.cad_High_CD44_High_19P4_AGM_VE.cad_High_CD44_High_35P4_AGM_VE.cad_High_CD44_High_19P4_AGM_VE.cad_High_CD44_High_35 P4_AGM_VE.cad_High_CD44_High_09P4_AGM_VE.cad_High_CD44_High_35P4_AGM_VE.cad_High_CD44_High_19P4_AGM_VE.cad_High_CD44_High_35 P4_AGM_VE.cad_High_CD44_High_09P4_AGM_VE.cad_High_CD44_High_09 MYOM1 P4_AGM_VE.cad_High_CD44_High_19P4_AGM_VE.cad_High_CD44_High_09

P4_AGM_VE.cad_Low_CD44._21P4_AGM_VE.cad_High_CD44_High_27P4_AGM_VE.cad_Low_CD44._21P4_AGM_VE.cad_High_CD44_High_27 P4_AGM_VE.cad_Low_CD44._21P4_AGM_VE.cad_High_CD44_High_27P4_AGM_VE.cad_Low_CD44._21P4_AGM_VE.cad_High_CD44_High_27

P4_AGM_VE.cad_Low_CD44._02P4_AGM_VE.cad_Low_CD44._24P4_AGM_VE.cad_Low_CD44._24 P4_AGM_VE.cad_Low_CD44._02P4_AGM_VE.cad_Low_CD44._24 P4_AGM_VE.cad_Low_CD44._02 SERPINE1 P4_AGM_VE.cad_Low_CD44._24P4_AGM_VE.cad_Low_CD44._02 P4_AGM_VE.cad_High_CD44_High_45P4_AGM_VE.cad_High_CD44_High_44P4_AGM_VE.cad_High_CD44_High_45P4_AGM_VE.cad_High_CD44_High_44 P3_AGM_VE.cad_High_CD44_low_24P4_AGM_VE.cad_High_CD44_High_44P4_AGM_VE.cad_High_CD44_High_45P4_AGM_VE.cad_High_CD44_High_44

P4_AGM_VE.cad_High_CD44_High_11P3_AGM_VE.cad_High_CD44_low_24P4_AGM_VE.cad_High_CD44_High_11P3_AGM_VE.cad_High_CD44_low_24 P4_AGM_VE.cad_High_CD44_High_11P4_AGM_VE.cad_High_CD44_High_45P3_AGM_VE.cad_High_CD44_low_24 P1_AGM_VE.cad._CD44._08P1_AGM_VE.cad._CD44._08 MET P1_AGM_VE.cad._CD44._08P1_AGM_VE.cad._CD44._08P4_AGM_VE.cad_High_CD44_High_11 P4_AGM_VE.cad_Low_CD44._10P4_AGM_VE.cad_Low_CD44._34P4_AGM_VE.cad_Low_CD44._10P4_AGM_VE.cad_Low_CD44._34 P4_AGM_VE.cad_Low_CD44._34P4_AGM_VE.cad_Low_CD44._10P4_AGM_VE.cad_Low_CD44._10P4_AGM_VE.cad_Low_CD44._34

P4_AGM_VE.cad_Low_CD44._27P4_AGM_VE.cad_Low_CD44._32P4_AGM_VE.cad_Low_CD44._32 P4_AGM_VE.cad_Low_CD44._27P4_AGM_VE.cad_Low_CD44._32 P4_AGM_VE.cad_Low_CD44._27 FMO1 P4_AGM_VE.cad_Low_CD44._32P4_AGM_VE.cad_Low_CD44._27

P4_AGM_VE.cad_Low_CD44._39P4_AGM_VE.cad_High_CD44_High_31P4_AGM_VE.cad_Low_CD44._39P4_AGM_VE.cad_High_CD44_High_31 P4_AGM_VE.cad_Low_CD44._11P4_AGM_VE.cad_High_CD44_High_31P4_AGM_VE.cad_Low_CD44._39P4_AGM_VE.cad_High_CD44_High_31

P4_AGM_VE.cad_High_CD44_High_20P4_AGM_VE.cad_Low_CD44._11P4_AGM_VE.cad_High_CD44_High_20P4_AGM_VE.cad_Low_CD44._11 P4_AGM_VE.cad_Low_CD44._39P4_AGM_VE.cad_Low_CD44._11 ITGB3 P4_AGM_VE.cad_High_CD44_High_20P4_AGM_VE.cad_High_CD44_High_20 P4_AGM_VE.cad_Low_CD44._41P4_AGM_VE.cad_Low_CD44._16P4_AGM_VE.cad_Low_CD44._41P4_AGM_VE.cad_Low_CD44._16 P4_AGM_VE.cad_Low_CD44._35P4_AGM_VE.cad_Low_CD44._16P4_AGM_VE.cad_Low_CD44._41P4_AGM_VE.cad_Low_CD44._16

P4_AGM_VE.cad_Low_CD44._44P4_AGM_VE.cad_Low_CD44._35P4_AGM_VE.cad_Low_CD44._44P4_AGM_VE.cad_Low_CD44._35 P1_AGM_VE.cad._CD44._18.1P4_AGM_VE.cad_Low_CD44._41P4_AGM_VE.cad_Low_CD44._44P4_AGM_VE.cad_Low_CD44._35 P1_AGM_VE.cad._CD44._18.1P1_AGM_VE.cad._CD44._18.1 GFI1 P4_AGM_VE.cad_Low_CD44._44P1_AGM_VE.cad._CD44._18.1 P4_AGM_VE.cad_Low_CD44._08P4_AGM_VE.cad_High_CD44_High_10P4_AGM_VE.cad_Low_CD44._08P4_AGM_VE.cad_High_CD44_High_10 P4_AGM_VE.cad_Low_CD44._08P4_AGM_VE.cad_High_CD44_High_10P4_AGM_VE.cad_Low_CD44._08P4_AGM_VE.cad_High_CD44_High_10

P4_AGM_VE.cad_High_CD44_High_23P4_AGM_VE.cad_Low_CD44._06P4_AGM_VE.cad_High_CD44_High_23P4_AGM_VE.cad_Low_CD44._06 P4_AGM_VE.cad_Low_CD44._06P4_AGM_VE.cad_Low_CD44._06

P4_AGM_VE.cad_High_CD44_High_25 GATA2 P4_AGM_VE.cad_High_CD44_High_23P4_AGM_VE.cad_High_CD44_High_23 P4_AGM_VE.cad_Low_CD44._25P4_AGM_VE.cad_Low_CD44._25P4_AGM_VE.cad_High_CD44_High_255 P4_AGM_VE.cad_Low_CD44._25P4_AGM_VE.cad_High_CD44_High_25P4_AGM_VE.cad_Low_CD44._25P4_AGM_VE.cad_High_CD44_High_25

P4_AGM_VE.cad_High_CD44_High_08P4_AGM_VE.cad_High_CD44_High_15P4_AGM_VE.cad_High_CD44_High_08P4_AGM_VE.cad_High_CD44_High_15 P4_AGM_VE.cad_High_CD44_High_08P4_AGM_VE.cad_High_CD44_High_15P4_AGM_VE.cad_High_CD44_High_08P4_AGM_VE.cad_High_CD44_High_15 P4_AGM_VE.cad_High_CD44_High_12P4_AGM_VE.cad_High_CD44_High_12 MYB 5 P4_AGM_VE.cad_High_CD44_High_12P4_AGM_VE.cad_High_CD44_High_12

P4_AGM_VE.cad_High_CD44_High_05P4_AGM_VE.cad_Low_CD44._20P4_AGM_VE.cad_High_CD44_High_05P4_AGM_VE.cad_Low_CD44._20 P4_AGM_VE.cad_Low_CD44._20P4_AGM_VE.cad_High_CD44_High_05P4_AGM_VE.cad_High_CD44_High_05P4_AGM_VE.cad_Low_CD44._20

P1_AGM_VE.cad._CD44._11.1P4_AGM_VE.cad_Low_CD44._01P1_AGM_VE.cad._CD44._11.1P4_AGM_VE.cad_Low_CD44._01 P1_AGM_VE.cad._CD44._11.1P4_AGM_VE.cad_Low_CD44._01 ATP2A3 P4_AGM_VE.cad_Low_CD44._01P1_AGM_VE.cad._CD44._11.1 P4_AGM_VE.cad_Low_CD44._05P4_AGM_VE.cad_Low_CD44._07P4_AGM_VE.cad_Low_CD44._05P4_AGM_VE.cad_Low_CD44._07 P4_AGM_VE.cad_Low_CD44._05P4_AGM_VE.cad_Low_CD44._07P4_AGM_VE.cad_Low_CD44._05P4_AGM_VE.cad_Low_CD44._07

P4_AGM_VE.cad_Low_CD44._43P4_AGM_VE.cad_High_CD44_High_07P4_AGM_VE.cad_Low_CD44._43P4_AGM_VE.cad_High_CD44_High_07 P4_AGM_VE.cad_Low_CD44._38P4_AGM_VE.cad_High_CD44_High_07P4_AGM_VE.cad_Low_CD44._43P4_AGM_VE.cad_High_CD44_High_07 P4_AGM_VE.cad_Low_CD44._38P4_AGM_VE.cad_Low_CD44._38 TGFB2 P4_AGM_VE.cad_Low_CD44._43P4_AGM_VE.cad_Low_CD44._38

P4_AGM_VE.cad_High_CD44_High_03P4_AGM_VE.cad_Low_CD44._42P4_AGM_VE.cad_High_CD44_High_03P4_AGM_VE.cad_Low_CD44._42 P4_AGM_VE.cad_Low_CD44._42P4_AGM_VE.cad_High_CD44_High_03P4_AGM_VE.cad_High_CD44_High_03P4_AGM_VE.cad_Low_CD44._42

P4_AGM_VE.cad_High_CD44_High_46P4_AGM_VE.cad_Low_CD44._14P4_AGM_VE.cad_Low_CD44._14 P4_AGM_VE.cad_Low_CD44._14P4_AGM_VE.cad_Low_CD44._14 P4_AGM_VE.cad_High_CD44_High_46 SHE P4_AGM_VE.cad_High_CD44_High_46P4_AGM_VE.cad_High_CD44_High_46 P1_AGM_VE.cad._CD44._02.1P4_AGM_VE.cad_Low_CD44._33P1_AGM_VE.cad._CD44._02.1P4_AGM_VE.cad_Low_CD44._33 P1_AGM_VE.cad._CD44._02.1P4_AGM_VE.cad_Low_CD44._33P1_AGM_VE.cad._CD44._02.1P4_AGM_VE.cad_Low_CD44._33

P4_AGM_VE.cad_High_CD44_High_02P4_AGM_VE.cad_High_CD44_High_42P4_AGM_VE.cad_High_CD44_High_02P4_AGM_VE.cad_High_CD44_High_42 P4_AGM_VE.cad_High_CD44_High_02P4_AGM_VE.cad_High_CD44_High_42P4_AGM_VE.cad_High_CD44_High_42 PDZD2 P4_AGM_VE.cad_High_CD44_High_02 P4_AGM_VE.cad_Low_CD44._29P1_AGM_VE.cad._CD44._15.1P4_AGM_VE.cad_Low_CD44._29P1_AGM_VE.cad._CD44._15.1 P1_AGM_VE.cad._CD44._10.1P1_AGM_VE.cad._CD44._15.1P4_AGM_VE.cad_Low_CD44._29P1_AGM_VE.cad._CD44._15.1

P4_AGM_VE.cad_Low_CD44._28P1_AGM_VE.cad._CD44._10.1P4_AGM_VE.cad_Low_CD44._28P1_AGM_VE.cad._CD44._10.1 P4_AGM_VE.cad_Low_CD44._28P4_AGM_VE.cad_Low_CD44._29P4_AGM_VE.cad_Low_CD44._28P1_AGM_VE.cad._CD44._10.1 P4_AGM_VE.cad_Low_CD44._13P4_AGM_VE.cad_Low_CD44._13 SNAI1 P4_AGM_VE.cad_High_CD44_High_22P4_AGM_VE.cad_Low_CD44._13

P4_AGM_VE.cad_Low_CD44._36P4_AGM_VE.cad_High_CD44_High_22P4_AGM_VE.cad_Low_CD44._36P4_AGM_VE.cad_High_CD44_High_22 P4_AGM_VE.cad_Low_CD44._36P4_AGM_VE.cad_Low_CD44._13P4_AGM_VE.cad_Low_CD44._36P4_AGM_VE.cad_High_CD44_High_22

P1_AGM_VE.cad._CD44._01.1P1_AGM_VE.cad._CD44._06P1_AGM_VE.cad._CD44._01.1P1_AGM_VE.cad._CD44._06 P1_AGM_VE.cad._CD44._06P1_AGM_VE.cad._CD44._06 SAMD4 P1_AGM_VE.cad._CD44._01.1P1_AGM_VE.cad._CD44._01.1 P4_AGM_VE.cad_High_CD44_High_13P4_AGM_VE.cad_High_CD44_High_38P4_AGM_VE.cad_High_CD44_High_13P4_AGM_VE.cad_High_CD44_High_38 P4_AGM_VE.cad_High_CD44_High_13P4_AGM_VE.cad_High_CD44_High_38P4_AGM_VE.cad_High_CD44_High_13P4_AGM_VE.cad_High_CD44_High_38

P4_AGM_VE.cad_High_CD44_High_18P4_AGM_VE.cad_Low_CD44._15P4_AGM_VE.cad_High_CD44_High_18P4_AGM_VE.cad_Low_CD44._15 P4_AGM_VE.cad_High_CD44_High_18P4_AGM_VE.cad_Low_CD44._15P4_AGM_VE.cad_High_CD44_High_18P4_AGM_VE.cad_Low_CD44._15 P4_AGM_VE.cad_High_CD44_High_30P4_AGM_VE.cad_High_CD44_High_39P4_AGM_VE.cad_High_CD44_High_30P4_AGM_VE.cad_High_CD44_High_39 LAT P4_AGM_VE.cad_High_CD44_High_30P4_AGM_VE.cad_High_CD44_High_39P4_AGM_VE.cad_High_CD44_High_30P4_AGM_VE.cad_High_CD44_High_39

P4_AGM_VE.cad_High_CD44_High_24P4_AGM_VE.cad_High_CD44_High_21P4_AGM_VE.cad_High_CD44_High_24P4_AGM_VE.cad_High_CD44_High_21 P4_AGM_VE.cad_High_CD44_High_24P4_AGM_VE.cad_High_CD44_High_21P4_AGM_VE.cad_High_CD44_High_24P4_AGM_VE.cad_High_CD44_High_21 BMP4

LAD1 ITGA2B

DCAF12L1

TGFBR2

PLCD1

ACVR1

PPP1R16B

ACVR2B

E9_5_23_to26sp

FAM122B E11_5_42_to45sp

E10_5_36_to39sp ACVR2A 15

defined by CD44, VE Supplementary Figure S2: S2: Figure Supplementary E10_30_to32sp

GroupID

15 Log2 Gene Expression Supplementary Figure S2: Single-cell q-RT-PCR analysis of the four populations defined by CD44, VE-Cad and Kit Single cells from indicated populations were isolated and tested for the expression of 95 genes by single-cell q-RT-PCR. The heatmap shows the result of the hierarchical clustering analysis (cells were clustered by Euclidian distance and the genes by Pearson correlation). Genes coding for Gata2, Runx1, Lyl1, Lmo2, Tal1, Fli1 and Erg transcription factors are specifically co-expressed in the CD44LowKitPos population but not in the other two (see genes indicated by arrows). See also Figure 3 and Supplementary File S2.

Oatley, Vargel-Bölükbası et al. 2018 Supplementary Figure S3: Results of single cell q-RT-PCR analysis of Pro-HSC, Pre-HSC-I and Pre-HSC-II a Log2 Gene Expression GroupID GroupID Pre_I PPIA 14 Sox6 14 Pre_II CDH2 Pro COL4A5 PCDH12 1212 GPR126 Pde2a Pag1 BMP4 1010 SNAI1 GATA1 GFI1B 88 HBB−BH1 EPOR Col3a1 CXCL12 66 HMMR Lrp1 ITGAM 44 Gpx1 FLI1 TGFB1 CD47 22 CD44 EMB Csf1r 0 PTPRC 0 Dock8 CORO1A SFPI1 Group ID Ly6e Laptm5 ALOX5AP PLA2G4A Pro-HSC Dock2 RUNX1 Ptpn6 Dapp1 Pre-HSC-I Ikzf1 Dok2 Was Notch2 Pre-HSC-II SLA LGALS9 SMAD2 SMAD3 Plcg2 LMO2 Ptk2b Rbm38 Cbfa2t3 TAL1 Nmt2 Stxbp2 Pfas Angpt1 Adgrg1 Ikzf2 KIT Bcl11a Mdk CDH5 PECAM1 ACTA2 Cbfb ATP2A3 Mctp1 Rab38 Med12l Mycn ITGB3 ITGA2B Dnmt3b Stk26 Nfe2 MYB GFI1 GATA2 ACVRL1 KDR TEK Emcn RAMP2 COL4A2 PTPRB Pde3a SERPINF1 MEIS2 Sox17 FBN1 SMAD7 NOTCH1 SMAD6 Pbx1 ESAM ERG P2_Pre.HSPCs.type.I_01_15 P2_Pre.HSPCs.type.I_01_08 P2_Pre.HSPCs.type.I_01_20 P2_Pre.HSPCs.type.I_01_38 P2_Pre.HSPCs.type.I_01_31 P2_Pre.HSPCs.type.I_01_02 P2_Pre.HSPCs.type.I_01_25 P2_Pre.HSPCs.type.I_01_41 P2_Pre.HSPCs.type.I_01_35 P2_Pre.HSPCs.type.I_01_36 P2_Pre.HSPCs.type.I_01_05 P2_Pre.HSPCs.type.I_01_43 P2_Pre.HSPCs.type.I_01_09 P2_Pro.HSPCs_01_22 P2_Pre.HSPCs.type.I_01_40 P1_Pro.HSPCs_01_11 P2_Pre.HSPCs.type.I_01_11 P2_Pre.HSPCs.type.I_01_21 P2_Pre.HSPCs.type.I_01_07 P2_Pre.HSPCs.type.I_01_24 P2_Pro.HSPCs_01_02 P2_Pre.HSPCs.type.I_01_14 P2_Pre.HSPCs.type.I_01_17 P2_Pro.HSPCs_01_44 P2_Pre.HSPCs.type.I_01_33 P2_Pre.HSPCs.type.I_01_26 P2_Pre.HSPCs.type.I_01_04 P2_Pre.HSPCs.type.I_01_10 P2_Pre.HSPCs.type.I_01_01 P2_Pro.HSPCs_01_10 P2_Pre.HSPCs.type.I_01_29 P2_Pre.HSPCs.type.I_01_44 P2_Pre.HSPCs.type.I_01_19 P2_Pre.HSPCs.type.I_01_39 P2_Pre.HSPCs.type.I_01_06 P2_Pre.HSPCs.type.I_01_16 P2_Pre.HSPCs.type.I_01_32 P2_Pro.HSPCs_01_09 P2_Pre.HSPCs.type.I_01_42 P2_Pro.HSPCs_01_17 P2_Pre.HSPCs.type.I_01_30 P2_Pre.HSPCs.type.I_01_23 P2_Pro.HSPCs_01_42 P2_Pro.HSPCs_01_16 P1_Pro.HSPCs_01_10 P1_Pre.HSPCs.type.I_01_07 P1_Pro.HSPCs_01_01 P2_Pro.HSPCs_01_12 P2_Pro.HSPCs_01_38 P2_Pro.HSPCs_01_41 P2_Pro.HSPCs_01_23 P2_Pro.HSPCs_01_04 P2_Pro.HSPCs_01_21 P2_Pro.HSPCs_01_19 P2_Pro.HSPCs_01_18 P2_Pro.HSPCs_01_29 P2_Pro.HSPCs_01_07 P2_Pro.HSPCs_01_20 P2_Pro.HSPCs_01_28 P2_Pro.HSPCs_01_03 P2_Pro.HSPCs_01_33 P2_Pro.HSPCs_01_40 P2_Pro.HSPCs_01_35 P2_Pre.HSPCs.type.I_01_22 P2_Pro.HSPCs_01_31 P1_Pro.HSPCs_01_13 P1_Pro.HSPCs_01_16 P1_Pro.HSPCs_01_12 P2_Pro.HSPCs_01_43 P2_Pro.HSPCs_01_05 P2_Pro.HSPCs_01_01 P1_Pre.HSPCs.type.II_01_26 P1_Pre.HSPCs.type.II_01_09 P1_Pre.HSPCs.type.II_01_11 P1_Pre.HSPCs.type.II_01_37 P1_Pre.HSPCs.type.II_01_53 P1_Pre.HSPCs.type.II_01_29 P1_Pre.HSPCs.type.II_01_03 P1_Pre.HSPCs.type.II_01_04 P1_Pre.HSPCs.type.II_01_10 P1_Pre.HSPCs.type.II_01_05 P1_Pre.HSPCs.type.II_01_47 P1_Pre.HSPCs.type.II_01_58 P1_Pre.HSPCs.type.II_01_33 P1_Pre.HSPCs.type.II_01_60 P1_Pre.HSPCs.type.II_01_32 P1_Pre.HSPCs.type.II_01_23 P1_Pre.HSPCs.type.II_01_18 P1_Pre.HSPCs.type.II_01_49 P1_Pre.HSPCs.type.II_01_02 P1_Pre.HSPCs.type.II_01_16 P1_Pre.HSPCs.type.II_01_07 P1_Pre.HSPCs.type.II_01_36 P1_Pre.HSPCs.type.II_01_27 P1_Pre.HSPCs.type.II_01_25 P1_Pre.HSPCs.type.II_01_34 P1_Pre.HSPCs.type.II_01_43 P1_Pre.HSPCs.type.II_01_28 P1_Pre.HSPCs.type.II_01_61 P1_Pre.HSPCs.type.II_01_21 P1_Pre.HSPCs.type.II_01_01 P1_Pre.HSPCs.type.II_01_45 P1_Pre.HSPCs.type.II_01_42 P1_Pre.HSPCs.type.II_01_51 P1_Pre.HSPCs.type.II_01_54 P1_Pre.HSPCs.type.II_01_17 P1_Pre.HSPCs.type.II_01_12 P1_Pre.HSPCs.type.II_01_35 P1_Pre.HSPCs.type.II_01_20 P1_Pre.HSPCs.type.II_01_30 P1_Pre.HSPCs.type.II_01_08 P1_Pre.HSPCs.type.II_01_24 P1_Pre.HSPCs.type.II_01_44 P1_Pre.HSPCs.type.II_01_41 P1_Pre.HSPCs.type.II_01_19 P1_Pre.HSPCs.type.II_01_48 P1_Pre.HSPCs.type.II_01_55 P1_Pre.HSPCs.type.II_01_46 P1_Pre.HSPCs.type.II_01_22 P1_Pre.HSPCs.type.II_01_38 P1_Pre.HSPCs.type.II_01_39 P1_Pre.HSPCs.type.II_01_52 P1_Pre.HSPCs.type.II_01_13 P1_Pre.HSPCs.type.II_01_40 P1_Pre.HSPCs.type.II_01_57 P1_Pre.HSPCs.type.II_01_15 P1_Pre.HSPCs.type.II_01_50 P1_Pre.HSPCs.type.II_01_06 P1_Pre.HSPCs.type.II_01_59 P1_Pre.HSPCs.type.II_01_14 P1_Pre.HSPCs.type.II_01_31 P1_Pre.HSPCs.type.II_01_56 P2_Pro.HSPCs_01_27 P2_Pro.HSPCs_01_37 P2_Pro.HSPCs_01_32 P2_Pro.HSPCs_01_24 P2_Pro.HSPCs_01_08 P2_Pro.HSPCs_01_39 P2_Pro.HSPCs_01_14 P2_Pro.HSPCs_01_06 P1_Pro.HSPCs_01_14 P2_Pro.HSPCs_01_36 P1_Pre.HSPCs.type.I_01_14 P1_Pre.HSPCs.type.I_01_11 P2_Pro.HSPCs_01_15 P1_Pre.HSPCs.type.I_01_15 P1_Pre.HSPCs.type.I_01_08 P2_Pro.HSPCs_01_25 P2_Pro.HSPCs_01_34 P2_Pro.HSPCs_01_13 P1_Pro.HSPCs_01_08 P1_Pre.HSPCs.type.I_01_06 P1_Pre.HSPCs.type.I_01_13 P1_Pro.HSPCs_01_15 P2_Pro.HSPCs_01_30 P1_Pro.HSPCs_01_06 P1_Pro.HSPCs_01_04 P1_Pre.HSPCs.type.I_01_05 b tSNE plot Group ID 20 Pro-HSC Pre-HSC-I Pre-HSC-II

samples$GroupID Pre_I Pre_II Pro Dimension 2

Dimension 2 Dimension 0

−10

−30 −20 −10 0 10 20 Dimension 1 Dimension 1 Oatley, Vargel-Bölükbası et al. 2018 Supplementary Figure S3: Results of single cell q-RT-PCR analysis of Pro-HSC, Pre-HSC-I and Pre-HSC-II (a) Single cells from Pro-HSCs (VE-Cad+ CD41+ CD45-CD43-), Pre-HSC-I (VE-Cad+ CD41+ CD45- CD43+), Pre-HSC-II (VE-Cad+ CD45+) populations were isolated and tested by single-cell q-RT-PCR. The heatmap shows the result of the hierarchical clustering analysis (cells were clustered by Euclidian distance and the genes by Pearson correlation). (b) tSNE plot from single cell q-RT-PCR data shown in (a). See also Figure 4 and Supplementary File S4.

Oatley, Vargel-Bölükbası et al. 2018 Pos et al. 2018 Kit Neg Low EC_CD44neg Pre_HSC_I Pre_HSC_II GroupID 3 2.5 2 1.5 1 0.5 0 Genes expressed by both endothelial and blood cells Blood genes specific CD44 genes specific CD44 genes specific Endothelial genes specific Bölükbası - Hacd4 Stat4 Pecam1 Cd47 Cbfb Lmo2 Gata2 Fli1 Lyl1 Cbfa2t3 Tal1 Notch1 Gpr126 Pde3a Snai1 Bmp4 Nr2f2 Nrp2 Pdlim1 Vwf Mycn Bcl11a Kit Angpt1 Gpr56 Gfi1 Mllt3 Pbx1 Itgb3 Acta2 Sox6 Smad6 Smad7 Sox17 Irf6 Fbn1 Hey2 Gja5 Jag1 Ctsc Ptprc Alox5ap Emb Dok2 Nfe2 Runx1 Cd44 Ikzf1 Gfi1b F10 Gcnt1 Rps6ka5 Ikzf2 GroupID Emcn Tek Fgd5 Cd93 Cdh5 Col4a2 Mdk Esam Ramp2 Dll4 Efnb2 Ptprb Pde2a Kdr Nrp1 Acvrl1 Ephb4 Flt4 Aplnr Ccnd1 Clec1b Lef1 Prl3b1 Cdh2 Stab2 Notch4 Pcdh12 Piezo2 T2_2712 T2_2413 T2_2705 seq dataset seq T2_2412 II - T2_2718 - T2_2717 T2_2415 T2_2407 T2_2709 Oatley, Vargel T2_2102 HSC T2_2719 -

RNA T2_2111 T2_2105 - T2_2113 T2_2103 Pre T2_2104 T2_3524 T2_2711 T2_3519 T2_3514 T2_3505 T2_3501 T2_3516 T1_3006 T2_3503 T2_3506 T1_2510 T1_2501 T1_2509 T1_2615 T1_3007 T1_2607 T2_2401 T1_2603 T1_3002 T1_2613 T1_3001 T1_2608 T1_3005 T2_2704 T2_2701 T2_2419 T2_2417 T2_2408 I T1_2614 - T2_2716 T2_3520 T2_2713 T2_3509 T2_2416

HSC T2_3525 - T2_3502 T2_3512 T2_3508 T2_3522 Pre T2_3511 T2_3504

Analysis of the Zhou et al. sc al. et Zhou the of Analysis T2_3523 T2_3521 T1_2504 T1_2606 T1_2602 T2_3510 T2_2418 T1_3009 GroupID GroupID T1_3010 T1CD201neg_3626EC_CD44neg Emcn T1_2508 T1_2616 Pre_HSC_I

33 Tek T1CD201neg_3612 EC_3211 Pre_HSC_II Fgd5 T1_3011 T1_2612 Cd93 T1_2604 EC_32222.5 Cdh5 EC_32092.5 Col4a2 T1CD201neg_3620 EC_3227 Mdk EC_3228 T1CD201neg_3615 22 Esam T1CD201neg_3617 T1CD201neg_3622 Ramp2 T1CD201neg_3609 T1_2610 Dll4 T1CD201neg_3621 T1CD201neg_36271.5 Efnb2 T1CD201neg_36111.5

Neg EC_3224 Ptprb EC_3207 Pde2a T1CD201neg_3616 EC_3208 11 Kdr T1CD201neg_3610 EC_3226 EC_3223

CD44 Nrp1 EC_3221 Acvrl1 EC_3213

(1+FKPM) Gene Expression T1_26050.5 Ephb4 EC_32170.5 10 EC_3216

Flt4 EC_3214

Aplnr EC_3219

Log Supplementary Figure S4: S4: Figure Supplementary EC_320500 Ccnd1 Clec1b Lef1 Prl3b1 Cdh2 Stab2 Notch4 Pcdh12 Piezo2 Snai1 Bmp4 Nr2f2 Nrp2 Pdlim1 Vwf Mycn Bcl11a Kit Angpt1 Gpr56 Gfi1 Mllt3 Pbx1 Acta2 Sox6 Smad6 Smad7 Sox17 Irf6 Fbn1 Hey2 Gja5 Jag1 Ctsc Ptprc Alox5ap Emb Dok2 Nfe2 Runx1 Cd44 Ikzf1 Gfi1b F10 Gcnt1 Rps6ka5 Ikzf2 Itgb3 Hacd4 Stat4 Pecam1 Cd47 Cbfb Lmo2 Gata2 Fli1 Lyl1 Cbfa2t3 Tal1 Notch1 Gpr126 Pde3a T2_2407 T2_2415 T2_2717 T2_2718 T2_2412 T2_2705 T2_2413 T2_2712 T2_3504 T2_3511 T2_3522 T2_3508 T2_3512 T2_3502 T2_3525 T2_2416 T2_3509 T2_2713 T2_3520 T2_2716 T1_2614 T2_2408 T2_2417 T2_2419 T2_2701 T2_2704 T1_3005 T1_2608 T1_3001 T1_2613 T1_3002 T1_2603 T2_2401 T1_2607 T1_3007 T1_2615 T1_2509 T1_2501 T1_2510 T2_3506 T2_3503 T1_3006 T2_3516 T2_3501 T2_3505 T2_3514 T2_3519 T2_2711 T2_3524 T2_2104 T2_2103 T2_2113 T2_2105 T2_2111 T2_2719 T2_2102 T2_2709 EC_3205 EC_3219 EC_3214 EC_3216 EC_3217 T1_2605 EC_3213 EC_3221 EC_3223 EC_3226 T1CD201neg_3610 EC_3208 T1CD201neg_3616 EC_3207 EC_3224 T1CD201neg_3611 T1CD201neg_3627 T1CD201neg_3621 T1_2610 T1CD201neg_3609 T1CD201neg_3622 T1CD201neg_3617 T1CD201neg_3615 EC_3228 EC_3227 T1CD201neg_3620 EC_3209 EC_3222 T1_2604 T1_2612 T1_3011 EC_3211 T1CD201neg_3612 T1_2616 T1_2508 T1CD201neg_3626 T1_3010 T1_3009 T2_2418 T2_3510 T1_2602 T1_2606 T1_2504 T2_3521 T2_3523 Supplementary Figure S4: Analysis of the Zhou et al. sc-RNA-seq dataset The heatmap shows the expression pattern of genes selected from Fig. 3 and Fig. 5 in the single cells studied by Zhou et al. 14 using sc-RNA-seq. These cells were isolated from E11 mouse embryos and included endothelial cells (CD44 Neg), Pre-HSC-I and Pre-HSC-II. The genes were grouped in the five indicated categories. See also Supplementary File S6.

Oatley, Vargel-Bölükbası et al. 2018

Rx1ko_CD44low.C.kit._01_25

Rx1ko_CD44low.C.kit._01_05.1

P2_CD44low.C.kit._01_12

Rx1ko_CD44low.C.kit._01_30

Rx1ko_CD44low.C.kit._01_31

Rx1ko_CD44low.C.kit._01_12

Rx1ko_CD44low.C.kit._01_11

Rx1ko_CD44low.C.kit._01_21

Rx1ko_CD44low.C.kit._01_01

Rx1ko_CD44low.C.kit._01_16

P1_CD44low.C.kit._01_17

P2_CD44low.C.kit._01_13

P2_CD44low.C.kit._01_04

Rx1ko_CD44low.C.kit._01_35

Rx1ko_CD44low.C.kit._01_13

Rx1ko_CD44low.C.kit._01_18

Rx1ko_CD44low.C.kit._01_02

Rx1ko_CD44low.C.kit._01_22

Rx1ko_CD44low.C.kit._01_10

Rx1ko_CD44low.C.kit._01_33

Rx1ko_CD44low.C.kit._01_06

Rx1ko_CD44low.C.kit._01_15

Rx1ko_CD44low.C.kit._01_04.1

Rx1ko_CD44low.C.kit._01_05

Rx1ko_CD44neg_01_09

Rx1ko_CD44neg_01_03

Rx1ko_CD44neg_01_10

P1_CD44low.C.kit._01_02

P1_CD44low.C.kit._01_18

P2_CD44low.C.kit._01_06

P1_CD44low.C.kit._01_01

P2_CD44low.C.kit._01_11

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P2_CD44low.C.kit._01_15

P2_CD44low.C.kit._01_09

P1_CD44low.C.kit._01_13.1

Rx1ko_CD44low.C.kit._01_14

Rx1ko_CD44low.C.kit._01_01.1

Rx1ko_CD44low.C.kit._01_02.1

Rx1ko_CD44low.C.kit._01_27

Rx1ko_CD44low.C.kit._01_32

Rx1ko_CD44low.C.kit._01_24

Rx1ko_CD44low.C.kit._01_26

P1_nokitP7_.1._01

P1_nokitP7_.1._22

P2_CD44low.C.kit._01_01

P2_CD44low.C.kit._01_02

P1_CD44low.C.kit._01_06

P2_CD44low.C.kit._01_08

P1_CD44low.C.kit._01_15

P1_CD44low.C.kit._01_21

P1_CD44low.C.kit._01_19

P2_CD44low.C.kit._01_10

P1_CD44low.C.kit._01_22

P2_CD44low.C.kit._01_07

Rx1ko_CD44low.C.kit._01_04

Rx1ko_CD44low.C.kit._01_17

Rx1ko_CD44low.C.kit._01_29

P1_CD44low.C.kit._01_08

P1_nokitP7_.1._06

P1_nokitP7_.1._16

P1_nokitP7_.1._23

P1_nokitP7_.1._02

P1_nokitP7_.1._04

P1_nokitP7_.1._19

P1_nokitP7_.1._24

Rx1ko_CD44low.C.kit._01_09 Supplementary Figure S5: Results of single cell q-RT-PCR analysis in the wild

Rx1ko_CD44low.C.kit._01_08

Rx1ko_CD44low.C.kit._01_19

P1_CD44low.C.kit._01_16

Rx1ko_CD44low.C.kit._01_07

P2_CD44low.C.kit._01_14

Rx1ko_CD44low.C.kit._01_23 -/-

P1_nokitP7_.1._08

P1_nokitP7_.1._17

P1_nokitP7_.1._13 type and Runx1 AGM

P1_nokitP7_.1._18

Rx1ko_CD44low.C.kit._01_03

P2_CD44low.C.kit._01_17

P1_nokitP7_.1._11

P1_nokitP7_.1._20

P1_nokitP7_.1._10

P1_nokitP7_.1._14

P1_nokitP7_.1._05

P1_nokitP7_.1._21

Rx1ko_CD44low.C.kit._01_20

P1_CD44low.C.kit._01_12

P2_CD44low.C.kit._01_05

P1_CD44low.C.kit._01_05

P1_CD44low.C.kit._01_07

P1_CD44low.C.kit._01_10

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P2_CD44low.C.kit._01_03

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P1_CD44low.C.kit._01_03

P1_CD44low.C.kit._01_09

P1_CD44low.C.kit._01_11

Rx1ko_CD44low.C.kit._01_34

P1_nokitP7_.1._07

P1_nokitP7_.1._12

P1_CD44neg_01_12

P1_CD44neg_01_14

P1_CD44neg_01_07

P1_CD44neg_01_22

P1_CD44neg_01_23

P1_CD44neg_01_06

P1_CD44neg_01_21

P1_CD44neg_01_19 GroupID GroupID P1_kitP7_.1._09

P1_kitP5_.1._01 14 Rx1ko_44lo_Kitneg

P1_kitP5_.1._19 PPIA P1_kitP5_.1._13

P1_kitP5_.1._12 Emcn Rx1ko_44lo_Kitpos P1_kitP7_.1._08 P1_kitP5_.1._03 KDR

Rx1ko_CD44neg

P1_nokitP7_.1._15 RAMP2 P1_kitP5_.1._02

P1_kitP7_.1._20 COL4A2 12 SC_1 P1_kitP5_.1._15

P1_kitP5_.1._20 TEK

P1_CD44neg_01_15 CDH5 SC_2 P1_kitP7_.1._21

P1_kitP7_.1._11 Mdk SC_3 P1_kitP7_.1._12

P1_kitP7_.1._16 PECAM1 10

P1_kitP7_.1._02 Dock8 SC_4 P1_kitP5_.1._07

P1_kitP7_.1._17 Stxbp2 SC_5 P1_kitP5_.1._21

P1_kitP5_.1._09 Ly6e

P1_kitP5_.1._22 Csf1r

P1_kitP5_.1._10 8

P1_kitP5_.1._08 PTPRC P1_kitP7_.1._03

P1_CD44low.C.kit._01_21.1 LGALS9

Rx1ko_CD44low.C.kit._01_08.1 Dock2 P1_kitP5_.1._11

P1_nokitP7_.1._03 Ikzf1

Rx1ko_CD44low.C.kit._01_06.1 6

Rx1ko_CD44low.C.kit._01_07.1 SLA

P1_kitP7_.1._22 Ptpn6 P1_kitP7_.1._19

Rx1ko_CD44neg_01_06 Dok2 Rx1ko_CD44low.C.kit._01_03.1

Rx1ko_CD44neg_01_11 RUNX1

P1_CD44neg_01_13 Was 4 P1_CD44neg_01_20

Rx1ko_CD44neg_01_01 PLA2G4A Rx1ko_CD44neg_01_07

Rx1ko_CD44neg_01_08 CORO1A

P1_CD44neg_01_09 SFPI1 P1_CD44neg_01_17

P1_CD44neg_01_02 ALOX5AP 2 Rx1ko_CD44neg_01_13

Rx1ko_CD44neg_01_12 Dapp1

P1_kitP5_.1._14 FLI1 P1_kitP5_.1._17

P1_kitP5_.1._05 TGFB1 P1_kitP5_.1._06

P1_kitP5_.1._18 LMO2

P1_kitP5_.1._04 Nmt2 0 P1_kitP5_.1._16

P1_CD44neg_01_10 Gpx1 P1_CD44neg_01_05

P1_CD44neg_01_16 CD47

P1_CD44neg_01_01 Pfas P1_CD44neg_01_08

Rx1ko_CD44neg_01_04 Laptm5 Rx1ko_CD44neg_01_14

Rx1ko_CD44neg_01_02 CD44

Rx1ko_CD44neg_01_05 EMB P1_kitP6_.1._09

P1_kitP6_.1._10 Adgrg1 P1_kitP6_.1._19

P1_CD44high_01_17 KIT

P1_kitP6_.1._13 Rbm38 P1_kitP6_.1._24

P1_kitP6_.1._04 TAL1 P1_kitP6_.1._15

P1_CD44high_01_23 SMAD2

P1_kitP6_.1._23 SMAD3 P1_kitP6_.1._03

P1_CD44high_01_10 MEIS2 P1_CD44high_01_18

P1_CD44high_01_02 SMAD6

P1_CD44high_01_16

P1_CD44low.C.kit._01_03.1 SMAD7

P1_kitP6_.1._20 Bcl11a P1_kitP6_.1._07

P1_kitP6_.1._05 Pbx1

P1_kitP6_.1._17

P1_kitP6_.1._08 Cbfa2t3

P1_kitP6_.1._12 ERG P1_kitP6_.1._22

P1_CD44high_01_08 FBN1

P1_CD44high_01_01

P1_CD44high_01_07 Pde3a

P1_CD44high_01_09 COL4A5 P1_CD44high_01_19

P1_CD44high_01_11 GPR126 P1_CD44high_01_20

P1_CD44low.C.kit._01_04 CDH2

P2_CD44low.C.kit._01_11.1 PCDH12 P1_CD44high_01_13

P1_CD44high_01_15 ACVRL1 P1_CD44high_01_03

P1_CD44high_01_04 Sox17

P1_CD44high_01_21 ESAM P2_CD44low.C.kit._01_09.1

P1_CD44high_01_06 PTPRB P1_CD44high_01_12

P1_kitP6_.1._11 Pde2a

P1_kitP6_.1._21 BMP4 P1_kitP7_.1._01

P1_nokitP7_.1._09 SNAI1 P1_kitP6_.1._01

P1_CD44low.C.kit._01_14 GATA1

P1_CD44low.C.kit._01_01.1 Mycn P1_CD44low.C.kit._01_05.1

P2_CD44low.C.kit._01_02.1 HBB−BH1 P1_CD44low.C.kit._01_09.1

P1_CD44high_01_05 EPOR

P1_CD44high_01_24 GFI1B P1_CD44high_01_22

P1_kitP6_.1._02 Pag1 P1_kitP7_.1._15

P1_CD44low.C.kit._01_07.1 ITGAM

P1_kitP6_.1._06 Lrp1 P1_CD44low.C.kit._01_02.1

P1_CD44low.C.kit._01_22.1 HMMR P2_CD44low.C.kit._01_14.1

P2_CD44low.C.kit._01_08.1 Notch2

P2_CD44low.C.kit._01_17.1 Col3a1 P2_CD44low.C.kit._01_03.1

P2_CD44low.C.kit._01_10.1 CXCL12 P1_kitP7_.1._23

P1_kitP6_.1._18 NOTCH1

P1_kitP7_.1._06 Cbfb P1_kitP7_.1._14

P1_kitP7_.1._10 GATA2 P1_kitP7_.1._13

P1_CD44low.C.kit._01_15.1 Dnmt3b

P1_CD44high_01_14 ITGA2B P1_CD44low.C.kit._01_04.1

P1_CD44low.C.kit._01_10.1 Nfe2 P2_CD44low.C.kit._01_16

P1_kitP7_.1._04 GFI1

P1_kitP7_.1._07 Rab38 P2_CD44low.C.kit._01_06.1

P2_CD44low.C.kit._01_04.1 Stk26 P2_CD44low.C.kit._01_12.1

P2_CD44low.C.kit._01_18 Mctp1

P1_CD44low.C.kit._01_11.1 Med12l P1_CD44low.C.kit._01_20.1

P1_kitP7_.1._05 Plcg2 P1_kitP7_.1._18

P2_CD44low.C.kit._01_05.1 ATP2A3

P2_CD44low.C.kit._01_13.1 MYB P1_CD44low.C.kit._01_08.1

P1_CD44low.C.kit._01_19.1 Ptk2b P2_CD44low.C.kit._01_01.1

P2_CD44low.C.kit._01_07.1 Ikzf2

P2_CD44low.C.kit._01_15.1 Angpt1 P1_CD44low.C.kit._01_18.1

P1_CD44low.C.kit._01_06.1 ITGB3 P1_CD44low.C.kit._01_14.1

P1_CD44low.C.kit._01_12.1 ACTA2

P1_CD44low.C.kit._01_16.1 P1_CD44low.C.kit._01_23.1 SERPINF1 Sox6 Rx1ko_CD44low.C.kit._01_25 Rx1ko_CD44low.C.kit._01_05.1 P2_CD44low.C.kit._01_12 Rx1ko_CD44low.C.kit._01_30 Rx1ko_CD44low.C.kit._01_31 Rx1ko_CD44low.C.kit._01_12 Rx1ko_CD44low.C.kit._01_11 Rx1ko_CD44low.C.kit._01_21 Rx1ko_CD44low.C.kit._01_01 Rx1ko_CD44low.C.kit._01_16 P1_CD44low.C.kit._01_17 P2_CD44low.C.kit._01_13 P2_CD44low.C.kit._01_04 Rx1ko_CD44low.C.kit._01_35 Rx1ko_CD44low.C.kit._01_13 Rx1ko_CD44low.C.kit._01_18 Rx1ko_CD44low.C.kit._01_02 Rx1ko_CD44low.C.kit._01_22 Rx1ko_CD44low.C.kit._01_10 Rx1ko_CD44low.C.kit._01_33 Rx1ko_CD44low.C.kit._01_06 Rx1ko_CD44low.C.kit._01_15 Rx1ko_CD44low.C.kit._01_04.1 Rx1ko_CD44low.C.kit._01_05 Rx1ko_CD44neg_01_09 Rx1ko_CD44neg_01_03 Rx1ko_CD44neg_01_10 P1_CD44low.C.kit._01_02 P1_CD44low.C.kit._01_18 P2_CD44low.C.kit._01_06 P1_CD44low.C.kit._01_01 P2_CD44low.C.kit._01_11 P1_CD44low.C.kit._01_23 P2_CD44low.C.kit._01_15 P2_CD44low.C.kit._01_09 P1_CD44low.C.kit._01_13.1 Rx1ko_CD44low.C.kit._01_14 Rx1ko_CD44low.C.kit._01_01.1 Rx1ko_CD44low.C.kit._01_02.1 Rx1ko_CD44low.C.kit._01_27 Rx1ko_CD44low.C.kit._01_32 Rx1ko_CD44low.C.kit._01_24 Rx1ko_CD44low.C.kit._01_26 P1_nokitP7_.1._01 P1_nokitP7_.1._22 P2_CD44low.C.kit._01_01 P2_CD44low.C.kit._01_02 P1_CD44low.C.kit._01_06 P2_CD44low.C.kit._01_08 P1_CD44low.C.kit._01_15 P1_CD44low.C.kit._01_21 P1_CD44low.C.kit._01_19 P2_CD44low.C.kit._01_10 P1_CD44low.C.kit._01_22 P2_CD44low.C.kit._01_07 Rx1ko_CD44low.C.kit._01_04 Rx1ko_CD44low.C.kit._01_17 Rx1ko_CD44low.C.kit._01_29 P1_CD44low.C.kit._01_08 P1_nokitP7_.1._06 P1_nokitP7_.1._16 P1_nokitP7_.1._23 P1_nokitP7_.1._02 P1_nokitP7_.1._04 P1_nokitP7_.1._19 P1_nokitP7_.1._24 Rx1ko_CD44low.C.kit._01_09 Rx1ko_CD44low.C.kit._01_08 Rx1ko_CD44low.C.kit._01_19 P1_CD44low.C.kit._01_16 Rx1ko_CD44low.C.kit._01_07 P2_CD44low.C.kit._01_14 Rx1ko_CD44low.C.kit._01_23 P1_nokitP7_.1._08 P1_nokitP7_.1._17 P1_nokitP7_.1._13 P1_nokitP7_.1._18 Rx1ko_CD44low.C.kit._01_03 P2_CD44low.C.kit._01_17 P1_nokitP7_.1._11 P1_nokitP7_.1._20 P1_nokitP7_.1._10 P1_nokitP7_.1._14 P1_nokitP7_.1._05 P1_nokitP7_.1._21 Rx1ko_CD44low.C.kit._01_20 P1_CD44low.C.kit._01_12 P2_CD44low.C.kit._01_05 P1_CD44low.C.kit._01_05 P1_CD44low.C.kit._01_07 P1_CD44low.C.kit._01_10 P1_CD44low.C.kit._01_20 P2_CD44low.C.kit._01_03 P1_CD44low.C.kit._01_13 P1_CD44low.C.kit._01_03 P1_CD44low.C.kit._01_09 P1_CD44low.C.kit._01_11 Rx1ko_CD44low.C.kit._01_34 P1_nokitP7_.1._07 P1_nokitP7_.1._12 P1_CD44neg_01_12 P1_CD44neg_01_14 P1_CD44neg_01_07 P1_CD44neg_01_22 P1_CD44neg_01_23 P1_CD44neg_01_06 P1_CD44neg_01_21 P1_CD44neg_01_19 P1_kitP7_.1._09 P1_kitP5_.1._01 P1_kitP5_.1._19 P1_kitP5_.1._13 P1_kitP5_.1._12 P1_kitP7_.1._08 P1_kitP5_.1._03 P1_nokitP7_.1._15 P1_kitP5_.1._02 P1_kitP7_.1._20 P1_kitP5_.1._15 P1_kitP5_.1._20 P1_CD44neg_01_15 P1_kitP7_.1._21 P1_kitP7_.1._11 P1_kitP7_.1._12 P1_kitP7_.1._16 P1_kitP7_.1._02 P1_kitP5_.1._07 P1_kitP7_.1._17 P1_kitP5_.1._21 P1_kitP5_.1._09 P1_kitP5_.1._22 P1_kitP5_.1._10 P1_kitP5_.1._08 P1_kitP7_.1._03 P1_CD44low.C.kit._01_21.1 Rx1ko_CD44low.C.kit._01_08.1 P1_kitP5_.1._11 P1_nokitP7_.1._03 Rx1ko_CD44low.C.kit._01_06.1 Rx1ko_CD44low.C.kit._01_07.1 P1_kitP7_.1._22 P1_kitP7_.1._19 Rx1ko_CD44neg_01_06 Rx1ko_CD44low.C.kit._01_03.1 Rx1ko_CD44neg_01_11 P1_CD44neg_01_13 P1_CD44neg_01_20 Rx1ko_CD44neg_01_01 Rx1ko_CD44neg_01_07 Rx1ko_CD44neg_01_08 P1_CD44neg_01_09 P1_CD44neg_01_17 P1_CD44neg_01_02 Rx1ko_CD44neg_01_13 Rx1ko_CD44neg_01_12 P1_kitP5_.1._14 P1_kitP5_.1._17 P1_kitP5_.1._05 P1_kitP5_.1._06 P1_kitP5_.1._18 P1_kitP5_.1._04 P1_kitP5_.1._16 P1_CD44neg_01_10 P1_CD44neg_01_05 P1_CD44neg_01_16 P1_CD44neg_01_01 P1_CD44neg_01_08 Rx1ko_CD44neg_01_04 Rx1ko_CD44neg_01_14 Rx1ko_CD44neg_01_02 Rx1ko_CD44neg_01_05 P1_kitP6_.1._09 P1_kitP6_.1._10 P1_kitP6_.1._19 P1_CD44high_01_17 P1_kitP6_.1._13 P1_kitP6_.1._24 P1_kitP6_.1._04 P1_kitP6_.1._15 P1_CD44high_01_23 P1_kitP6_.1._23 P1_kitP6_.1._03 P1_CD44high_01_10 P1_CD44high_01_18 P1_CD44high_01_02 P1_CD44high_01_16 P1_CD44low.C.kit._01_03.1 P1_kitP6_.1._20 P1_kitP6_.1._07 P1_kitP6_.1._05 P1_kitP6_.1._17 P1_kitP6_.1._08 P1_kitP6_.1._12 P1_kitP6_.1._22 P1_CD44high_01_08 P1_CD44high_01_01 P1_CD44high_01_07 P1_CD44high_01_09 P1_CD44high_01_19 P1_CD44high_01_11 P1_CD44high_01_20 P1_CD44low.C.kit._01_04 P2_CD44low.C.kit._01_11.1 P1_CD44high_01_13 P1_CD44high_01_15 P1_CD44high_01_03 P1_CD44high_01_04 P1_CD44high_01_21 P2_CD44low.C.kit._01_09.1 P1_CD44high_01_06 P1_CD44high_01_12 P1_kitP6_.1._11 P1_kitP6_.1._21 P1_kitP7_.1._01 P1_nokitP7_.1._09 P1_kitP6_.1._01 P1_CD44low.C.kit._01_14 P1_CD44low.C.kit._01_01.1 P1_CD44low.C.kit._01_05.1 P2_CD44low.C.kit._01_02.1 P1_CD44low.C.kit._01_09.1 P1_CD44high_01_05 P1_CD44high_01_24 P1_CD44high_01_22 P1_kitP6_.1._02 P1_kitP7_.1._15 P1_CD44low.C.kit._01_07.1 P1_kitP6_.1._06 P1_CD44low.C.kit._01_02.1 P1_CD44low.C.kit._01_22.1 P2_CD44low.C.kit._01_14.1 P2_CD44low.C.kit._01_08.1 P2_CD44low.C.kit._01_17.1 P2_CD44low.C.kit._01_03.1 P2_CD44low.C.kit._01_10.1 P1_kitP7_.1._23 P1_kitP6_.1._18 P1_kitP7_.1._06 P1_kitP7_.1._14 P1_kitP7_.1. Sox6 SERPINF1 ACTA2 ITGB3 Angpt1 Ikzf2 Ptk2b MYB ATP2A3 Plcg2 Med12l Mctp1 Stk26 Rab38 GFI1 Nfe2 ITGA2B Dnmt3b GATA2 Cbfb NOTCH1 CXCL12 Col3a1 Notch2 HMMR Lrp1 ITGAM Pag1 GFI1B EPOR HBB Mycn GATA1 SNAI1 BMP4 Pde2a PTPRB ESAM Sox17 ACVRL1 PCDH12 CDH2 GPR126 COL4A5 Pde3a FBN1 ERG Cbfa2t3 Pbx1 Bcl11a SMAD7 SMAD6 MEIS2 SMAD3 SMAD2 TAL1 Rbm38 KIT Adgrg1 EMB CD44 Laptm5 Pfas CD47 Gpx1 Nmt2 LMO2 TGFB1 FLI1 Dapp1 ALOX5AP SFPI1 CORO1A PLA2G4A Was RUNX1 Dok2 Ptpn6 SLA Ikzf1 Dock2 LGALS9 PTPRC Csf1r Ly6e Stxbp2 Dock8 PECAM1 Mdk CDH5 TEK COL4A2 RAMP2 KDR Emcn PPIA GroupID − BH1 Log2 Gene expression Group ID Group ID Genotype SC_1 Runx1-/- CD44Neg 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 Runx1-/- Runx1-/- type Wild CD44neg Runx1-/- CD44lo_Kitpos CD44lo_Kitpos Runx1-/- CD44lo_Kitneg Runx1-/- SC_5 SC_5 SC_1 SC_4 SC_4 SC_3 SC_2

0 2 4 6 8 10 12 14 GroupID SC_2 Runx1-/- CD44LowKitNeg

SC_5 SC_4 SC_3 SC_2 SC_1 Rx1ko_CD44neg Rx1ko_44lo_Kitpos Rx1ko_44lo_Kitneg 2 Gene expression Gene Log

SC_3 Runx1-/-CD44LowKitPos SC_4

SC_5

Rx1ko_CD44low.C.kit._01_25 Rx1ko_CD44low.C.kit._01_25

Rx1ko_CD44low.C.kit._01_25

Rx1ko_CD44low.C.kit._01_05.1 Rx1ko_CD44low.C.kit._01_05.1

Rx1ko_CD44low.C.kit._01_05.1

P2_CD44low.C.kit._01_12 P2_CD44low.C.kit._01_12

P2_CD44low.C.kit._01_12

Rx1ko_CD44low.C.kit._01_30 Rx1ko_CD44low.C.kit._01_30

Rx1ko_CD44low.C.kit._01_30

Rx1ko_CD44low.C.kit._01_31 Rx1ko_CD44low.C.kit._01_31

Rx1ko_CD44low.C.kit._01_31

Rx1ko_CD44low.C.kit._01_12 Rx1ko_CD44low.C.kit._01_12

Rx1ko_CD44low.C.kit._01_12

Rx1ko_CD44low.C.kit._01_11 Rx1ko_CD44low.C.kit._01_11

Rx1ko_CD44low.C.kit._01_11

Rx1ko_CD44low.C.kit._01_21 Rx1ko_CD44low.C.kit._01_21

Rx1ko_CD44low.C.kit._01_21

Rx1ko_CD44low.C.kit._01_01 Rx1ko_CD44low.C.kit._01_01

Rx1ko_CD44low.C.kit._01_01

Rx1ko_CD44low.C.kit._01_16 Rx1ko_CD44low.C.kit._01_16

Rx1ko_CD44low.C.kit._01_16

P1_CD44low.C.kit._01_17 P1_CD44low.C.kit._01_17

P1_CD44low.C.kit._01_17

P2_CD44low.C.kit._01_13 P2_CD44low.C.kit._01_13

P2_CD44low.C.kit._01_13

P2_CD44low.C.kit._01_04 P2_CD44low.C.kit._01_04

P2_CD44low.C.kit._01_04

Rx1ko_CD44low.C.kit._01_35 Rx1ko_CD44low.C.kit._01_35

Rx1ko_CD44low.C.kit._01_35

Rx1ko_CD44low.C.kit._01_13 Rx1ko_CD44low.C.kit._01_13

Rx1ko_CD44low.C.kit._01_13

Rx1ko_CD44low.C.kit._01_18 Rx1ko_CD44low.C.kit._01_18

Rx1ko_CD44low.C.kit._01_18

Rx1ko_CD44low.C.kit._01_02 Rx1ko_CD44low.C.kit._01_02

Rx1ko_CD44low.C.kit._01_02

Rx1ko_CD44low.C.kit._01_22 Rx1ko_CD44low.C.kit._01_22

Rx1ko_CD44low.C.kit._01_22

Rx1ko_CD44low.C.kit._01_10 Rx1ko_CD44low.C.kit._01_10

Rx1ko_CD44low.C.kit._01_10

Rx1ko_CD44low.C.kit._01_33 Rx1ko_CD44low.C.kit._01_33

Rx1ko_CD44low.C.kit._01_33

Rx1ko_CD44low.C.kit._01_06 Rx1ko_CD44low.C.kit._01_06

Rx1ko_CD44low.C.kit._01_06

Rx1ko_CD44low.C.kit._01_15 Rx1ko_CD44low.C.kit._01_15

Rx1ko_CD44low.C.kit._01_15

Rx1ko_CD44low.C.kit._01_04.1 Rx1ko_CD44low.C.kit._01_04.1

Rx1ko_CD44low.C.kit._01_04.1

Rx1ko_CD44low.C.kit._01_05 Rx1ko_CD44low.C.kit._01_05

Rx1ko_CD44low.C.kit._01_05

Rx1ko_CD44neg_01_09 Rx1ko_CD44neg_01_09

Rx1ko_CD44neg_01_09

Rx1ko_CD44neg_01_03 Rx1ko_CD44neg_01_03

Rx1ko_CD44neg_01_03

Rx1ko_CD44neg_01_10 Rx1ko_CD44neg_01_10

Rx1ko_CD44neg_01_10

P1_CD44low.C.kit._01_02 P1_CD44low.C.kit._01_02

P1_CD44low.C.kit._01_02

P1_CD44low.C.kit._01_18 P1_CD44low.C.kit._01_18

P1_CD44low.C.kit._01_18

P2_CD44low.C.kit._01_06 P2_CD44low.C.kit._01_06

P2_CD44low.C.kit._01_06

P1_CD44low.C.kit._01_01 P1_CD44low.C.kit._01_01

P1_CD44low.C.kit._01_01

P2_CD44low.C.kit._01_11 P2_CD44low.C.kit._01_11

P2_CD44low.C.kit._01_11

P1_CD44low.C.kit._01_23 P1_CD44low.C.kit._01_23

P1_CD44low.C.kit._01_23

P2_CD44low.C.kit._01_15 P2_CD44low.C.kit._01_15

P2_CD44low.C.kit._01_15

P2_CD44low.C.kit._01_09 P2_CD44low.C.kit._01_09

P2_CD44low.C.kit._01_09

P1_CD44low.C.kit._01_13.1 P1_CD44low.C.kit._01_13.1

P1_CD44low.C.kit._01_13.1

Rx1ko_CD44low.C.kit._01_14 Rx1ko_CD44low.C.kit._01_14

Rx1ko_CD44low.C.kit._01_14

Rx1ko_CD44low.C.kit._01_01.1 Rx1ko_CD44low.C.kit._01_01.1

Rx1ko_CD44low.C.kit._01_01.1

Rx1ko_CD44low.C.kit._01_02.1 Rx1ko_CD44low.C.kit._01_02.1

Rx1ko_CD44low.C.kit._01_02.1

Rx1ko_CD44low.C.kit._01_27 Rx1ko_CD44low.C.kit._01_27

Rx1ko_CD44low.C.kit._01_27

Rx1ko_CD44low.C.kit._01_32 Rx1ko_CD44low.C.kit._01_32

Rx1ko_CD44low.C.kit._01_32

Rx1ko_CD44low.C.kit._01_24 Rx1ko_CD44low.C.kit._01_24

Rx1ko_CD44low.C.kit._01_24

Rx1ko_CD44low.C.kit._01_26 Rx1ko_CD44low.C.kit._01_26

Rx1ko_CD44low.C.kit._01_26

P1_nokitP7_.1._01 P1_nokitP7_.1._01

P1_nokitP7_.1._01

P1_nokitP7_.1._22 P1_nokitP7_.1._22

P1_nokitP7_.1._22

P2_CD44low.C.kit._01_01 P2_CD44low.C.kit._01_01

P2_CD44low.C.kit._01_01

P2_CD44low.C.kit._01_02 P2_CD44low.C.kit._01_02

P2_CD44low.C.kit._01_02

P1_CD44low.C.kit._01_06 P1_CD44low.C.kit._01_06

P1_CD44low.C.kit._01_06

P2_CD44low.C.kit._01_08 P2_CD44low.C.kit._01_08

P2_CD44low.C.kit._01_08

P1_CD44low.C.kit._01_15 P1_CD44low.C.kit._01_15

P1_CD44low.C.kit._01_15

P1_CD44low.C.kit._01_21 P1_CD44low.C.kit._01_21

P1_CD44low.C.kit._01_21

P1_CD44low.C.kit._01_19 P1_CD44low.C.kit._01_19

P1_CD44low.C.kit._01_19

P2_CD44low.C.kit._01_10 P2_CD44low.C.kit._01_10

P2_CD44low.C.kit._01_10

P1_CD44low.C.kit._01_22 P1_CD44low.C.kit._01_22

P1_CD44low.C.kit._01_22

P2_CD44low.C.kit._01_07 P2_CD44low.C.kit._01_07

P2_CD44low.C.kit._01_07

Rx1ko_CD44low.C.kit._01_04 Rx1ko_CD44low.C.kit._01_04

Rx1ko_CD44low.C.kit._01_04

Rx1ko_CD44low.C.kit._01_17 Rx1ko_CD44low.C.kit._01_17

Rx1ko_CD44low.C.kit._01_17

Rx1ko_CD44low.C.kit._01_29 Rx1ko_CD44low.C.kit._01_29

Rx1ko_CD44low.C.kit._01_29

P1_CD44low.C.kit._01_08 P1_CD44low.C.kit._01_08

P1_CD44low.C.kit._01_08

P1_nokitP7_.1._06 P1_nokitP7_.1._06

P1_nokitP7_.1._06

P1_nokitP7_.1._16 P1_nokitP7_.1._16

P1_nokitP7_.1._16

P1_nokitP7_.1._23 P1_nokitP7_.1._23

P1_nokitP7_.1._23

P1_nokitP7_.1._02 P1_nokitP7_.1._02

P1_nokitP7_.1._02

P1_nokitP7_.1._04 P1_nokitP7_.1._04

P1_nokitP7_.1._04

P1_nokitP7_.1._19 P1_nokitP7_.1._19

P1_nokitP7_.1._19

P1_nokitP7_.1._24 P1_nokitP7_.1._24

P1_nokitP7_.1._24

Rx1ko_CD44low.C.kit._01_09 Rx1ko_CD44low.C.kit._01_09

Rx1ko_CD44low.C.kit._01_09

Rx1ko_CD44low.C.kit._01_08 Rx1ko_CD44low.C.kit._01_08

Rx1ko_CD44low.C.kit._01_08

Rx1ko_CD44low.C.kit._01_19 Rx1ko_CD44low.C.kit._01_19

Rx1ko_CD44low.C.kit._01_19

P1_CD44low.C.kit._01_16 P1_CD44low.C.kit._01_16

P1_CD44low.C.kit._01_16

Rx1ko_CD44low.C.kit._01_07 Rx1ko_CD44low.C.kit._01_07

Rx1ko_CD44low.C.kit._01_07

P2_CD44low.C.kit._01_14 P2_CD44low.C.kit._01_14

P2_CD44low.C.kit._01_14

Rx1ko_CD44low.C.kit._01_23 Rx1ko_CD44low.C.kit._01_23

Rx1ko_CD44low.C.kit._01_23

P1_nokitP7_.1._08 P1_nokitP7_.1._08

P1_nokitP7_.1._08

P1_nokitP7_.1._17 P1_nokitP7_.1._17

P1_nokitP7_.1._17

P1_nokitP7_.1._13 P1_nokitP7_.1._13

P1_nokitP7_.1._13

P1_nokitP7_.1._18 P1_nokitP7_.1._18

P1_nokitP7_.1._18

Rx1ko_CD44low.C.kit._01_03 Rx1ko_CD44low.C.kit._01_03

Rx1ko_CD44low.C.kit._01_03

P2_CD44low.C.kit._01_17 P2_CD44low.C.kit._01_17

P2_CD44low.C.kit._01_17

P1_nokitP7_.1._11 P1_nokitP7_.1._11

P1_nokitP7_.1._11

P1_nokitP7_.1._20 P1_nokitP7_.1._20

P1_nokitP7_.1._20

P1_nokitP7_.1._10 P1_nokitP7_.1._10

P1_nokitP7_.1._10

P1_nokitP7_.1._14 P1_nokitP7_.1._14

P1_nokitP7_.1._14

P1_nokitP7_.1._05 P1_nokitP7_.1._05

P1_nokitP7_.1._05

P1_nokitP7_.1._21 P1_nokitP7_.1._21

P1_nokitP7_.1._21

Rx1ko_CD44low.C.kit._01_20 Rx1ko_CD44low.C.kit._01_20

Rx1ko_CD44low.C.kit._01_20

P1_CD44low.C.kit._01_12 P1_CD44low.C.kit._01_12

P1_CD44low.C.kit._01_12

P2_CD44low.C.kit._01_05 P2_CD44low.C.kit._01_05

P2_CD44low.C.kit._01_05

P1_CD44low.C.kit._01_05 P1_CD44low.C.kit._01_05

P1_CD44low.C.kit._01_05

P1_CD44low.C.kit._01_07 P1_CD44low.C.kit._01_07

P1_CD44low.C.kit._01_07

P1_CD44low.C.kit._01_10 P1_CD44low.C.kit._01_10

P1_CD44low.C.kit._01_10

P1_CD44low.C.kit._01_20 P1_CD44low.C.kit._01_20

P1_CD44low.C.kit._01_20

P2_CD44low.C.kit._01_03 P2_CD44low.C.kit._01_03

P2_CD44low.C.kit._01_03

P1_CD44low.C.kit._01_13 P1_CD44low.C.kit._01_13

P1_CD44low.C.kit._01_13

P1_CD44low.C.kit._01_03 P1_CD44low.C.kit._01_03

P1_CD44low.C.kit._01_03

P1_CD44low.C.kit._01_09 P1_CD44low.C.kit._01_09

P1_CD44low.C.kit._01_09

P1_CD44low.C.kit._01_11 P1_CD44low.C.kit._01_11

P1_CD44low.C.kit._01_11

Rx1ko_CD44low.C.kit._01_34 Rx1ko_CD44low.C.kit._01_34

Rx1ko_CD44low.C.kit._01_34

P1_nokitP7_.1._07 P1_nokitP7_.1._07

P1_nokitP7_.1._07

P1_nokitP7_.1._12 P1_nokitP7_.1._12

P1_nokitP7_.1._12

P1_CD44neg_01_12 P1_CD44neg_01_12

P1_CD44neg_01_12

P1_CD44neg_01_14 P1_CD44neg_01_14

P1_CD44neg_01_14

P1_CD44neg_01_07 P1_CD44neg_01_07

P1_CD44neg_01_07

P1_CD44neg_01_22

P1_CD44neg_01_23 P1_CD44neg_01_23

P1_CD44neg_01_23

P1_CD44neg_01_06

P1_CD44neg_01_21

P1_CD44neg_01_19

P1_kitP7_.1._09

P1_kitP5_.1._01

P1_kitP5_.1._19

P1_kitP5_.1._13

P1_kitP5_.1._12

P1_kitP7_.1._08

P1_kitP5_.1._03

P1_nokitP7_.1._15

P1_kitP5_.1._02 P1_kitP5_.1._02 P1_kitP5_.1._02

P1_kitP5_.1._02

P1_kitP7_.1._20

P1_kitP7_.1._20 P1_kitP7_.1._20

P1_kitP7_.1._20

P1_kitP5_.1._15 P1_kitP5_.1._15

P1_kitP5_.1._15

P1_kitP5_.1._20 P1_kitP5_.1._20 P1_kitP5_.1._20

P1_kitP5_.1._20

P1_CD44neg_01_15 P1_CD44neg_01_15 P1_CD44neg_01_15

P1_CD44neg_01_15

P1_kitP7_.1._21 P1_kitP7_.1._21 P1_kitP7_.1._21

P1_kitP7_.1._21

P1_kitP7_.1._11 P1_kitP7_.1._11 P1_kitP7_.1._11

P1_kitP7_.1._11

P1_kitP7_.1._12 P1_kitP7_.1._12 P1_kitP7_.1._12

P1_kitP7_.1._12

P1_kitP7_.1._16 P1_kitP7_.1._16 P1_kitP7_.1._16

P1_kitP7_.1._16

P1_kitP7_.1._02 P1_kitP7_.1._02 P1_kitP7_.1._02

P1_kitP7_.1._02

P1_kitP5_.1._07 P1_kitP5_.1._07 P1_kitP5_.1._07

P1_kitP5_.1._07

P1_kitP7_.1._17 P1_kitP7_.1._17 P1_kitP7_.1._17

P1_kitP7_.1._17

P1_kitP5_.1._21 P1_kitP5_.1._21 P1_kitP5_.1._21

P1_kitP5_.1._21

P1_kitP5_.1._09 P1_kitP5_.1._09 P1_kitP5_.1._09

P1_kitP5_.1._09

P1_kitP5_.1._22 P1_kitP5_.1._22 P1_kitP5_.1._22

P1_kitP5_.1._22

P1_kitP5_.1._10 P1_kitP5_.1._10 P1_kitP5_.1._10

P1_kitP5_.1._10

P1_kitP5_.1._08 P1_kitP5_.1._08 P1_kitP5_.1._08

P1_kitP5_.1._08

P1_kitP7_.1._03 P1_kitP7_.1._03 P1_kitP7_.1._03

P1_kitP7_.1._03

P1_CD44low.C.kit._01_21.1 P1_CD44low.C.kit._01_21.1 P1_CD44low.C.kit._01_21.1

P1_CD44low.C.kit._01_21.1

Rx1ko_CD44low.C.kit._01_08.1 Rx1ko_CD44low.C.kit._01_08.1 Rx1ko_CD44low.C.kit._01_08.1

Rx1ko_CD44low.C.kit._01_08.1

P1_kitP5_.1._11 P1_kitP5_.1._11 P1_kitP5_.1._11

P1_kitP5_.1._11

P1_nokitP7_.1._03 P1_nokitP7_.1._03 P1_nokitP7_.1._03

P1_nokitP7_.1._03

Rx1ko_CD44low.C.kit._01_06.1 Rx1ko_CD44low.C.kit._01_06.1 Rx1ko_CD44low.C.kit._01_06.1

Rx1ko_CD44low.C.kit._01_06.1

Rx1ko_CD44low.C.kit._01_07.1 Rx1ko_CD44low.C.kit._01_07.1 Rx1ko_CD44low.C.kit._01_07.1

Rx1ko_CD44low.C.kit._01_07.1

P1_kitP7_.1._22 P1_kitP7_.1._22 P1_kitP7_.1._22

P1_kitP7_.1._22

P1_kitP7_.1._19 P1_kitP7_.1._19 P1_kitP7_.1._19

P1_kitP7_.1._19

Rx1ko_CD44neg_01_06 Rx1ko_CD44neg_01_06 Rx1ko_CD44neg_01_06

Rx1ko_CD44neg_01_06

Rx1ko_CD44low.C.kit._01_03.1 Rx1ko_CD44low.C.kit._01_03.1 Rx1ko_CD44low.C.kit._01_03.1

Rx1ko_CD44low.C.kit._01_03.1

Rx1ko_CD44neg_01_11 Rx1ko_CD44neg_01_11 Rx1ko_CD44neg_01_11

Rx1ko_CD44neg_01_11

P1_CD44neg_01_13 P1_CD44neg_01_13 P1_CD44neg_01_13

P1_CD44neg_01_13

P1_CD44neg_01_20 P1_CD44neg_01_20 P1_CD44neg_01_20

P1_CD44neg_01_20

Rx1ko_CD44neg_01_01 Rx1ko_CD44neg_01_01 Rx1ko_CD44neg_01_01

Rx1ko_CD44neg_01_01

Rx1ko_CD44neg_01_07 Rx1ko_CD44neg_01_07 Rx1ko_CD44neg_01_07

Rx1ko_CD44neg_01_07

Rx1ko_CD44neg_01_08 Rx1ko_CD44neg_01_08 Rx1ko_CD44neg_01_08

Rx1ko_CD44neg_01_08

P1_CD44neg_01_09 P1_CD44neg_01_09 P1_CD44neg_01_09

P1_CD44neg_01_09

P1_CD44neg_01_17 P1_CD44neg_01_17 P1_CD44neg_01_17

P1_CD44neg_01_17

P1_CD44neg_01_02 P1_CD44neg_01_02 P1_CD44neg_01_02

P1_CD44neg_01_02

Rx1ko_CD44neg_01_13 Rx1ko_CD44neg_01_13 Rx1ko_CD44neg_01_13

Rx1ko_CD44neg_01_13

Rx1ko_CD44neg_01_12 Rx1ko_CD44neg_01_12 Rx1ko_CD44neg_01_12

Rx1ko_CD44neg_01_12

P1_kitP5_.1._14 P1_kitP5_.1._14 P1_kitP5_.1._14

P1_kitP5_.1._14

P1_kitP5_.1._17 P1_kitP5_.1._17 P1_kitP5_.1._17

P1_kitP5_.1._17

P1_kitP5_.1._05 P1_kitP5_.1._05 P1_kitP5_.1._05

P1_kitP5_.1._05

P1_kitP5_.1._06 P1_kitP5_.1._06 P1_kitP5_.1._06

P1_kitP5_.1._06

P1_kitP5_.1._18 P1_kitP5_.1._18 P1_kitP5_.1._18

P1_kitP5_.1._18

P1_kitP5_.1._04 P1_kitP5_.1._04 P1_kitP5_.1._04

P1_kitP5_.1._04

P1_kitP5_.1._16 P1_kitP5_.1._16 P1_kitP5_.1._16

P1_kitP5_.1._16

P1_CD44neg_01_10 P1_CD44neg_01_10 P1_CD44neg_01_10

P1_CD44neg_01_10

P1_CD44neg_01_05 P1_CD44neg_01_05 P1_CD44neg_01_05

P1_CD44neg_01_05

P1_CD44neg_01_16 P1_CD44neg_01_16 P1_CD44neg_01_16

P1_CD44neg_01_16

P1_CD44neg_01_01 P1_CD44neg_01_01 P1_CD44neg_01_01

P1_CD44neg_01_01

P1_CD44neg_01_08 P1_CD44neg_01_08 P1_CD44neg_01_08

P1_CD44neg_01_08

Rx1ko_CD44neg_01_04 Rx1ko_CD44neg_01_04 Rx1ko_CD44neg_01_04

Rx1ko_CD44neg_01_04

Rx1ko_CD44neg_01_14 Rx1ko_CD44neg_01_14 Rx1ko_CD44neg_01_14

Rx1ko_CD44neg_01_14

Rx1ko_CD44neg_01_02 Rx1ko_CD44neg_01_02 Rx1ko_CD44neg_01_02

Rx1ko_CD44neg_01_02

Rx1ko_CD44neg_01_05 Rx1ko_CD44neg_01_05 Rx1ko_CD44neg_01_05

Rx1ko_CD44neg_01_05 Oatley, Vargel-Bölükbası et al. 2018 P1_kitP6_.1._09 P1_kitP6_.1._09 P1_kitP6_.1._09

P1_kitP6_.1._09

P1_kitP6_.1._10 P1_kitP6_.1._10 P1_kitP6_.1._10

P1_kitP6_.1._10

P1_kitP6_.1._19 P1_kitP6_.1._19 P1_kitP6_.1._19

P1_kitP6_.1._19

P1_CD44high_01_17 P1_CD44high_01_17 P1_CD44high_01_17

P1_CD44high_01_17

P1_kitP6_.1._13 P1_kitP6_.1._13 P1_kitP6_.1._13

P1_kitP6_.1._13

P1_kitP6_.1._24 P1_kitP6_.1._24 P1_kitP6_.1._24

P1_kitP6_.1._24

P1_kitP6_.1._04 P1_kitP6_.1._04 P1_kitP6_.1._04

P1_kitP6_.1._04

P1_kitP6_.1._15 P1_kitP6_.1._15 P1_kitP6_.1._15

P1_kitP6_.1._15

P1_CD44high_01_23 P1_CD44high_01_23 P1_CD44high_01_23

P1_CD44high_01_23

P1_kitP6_.1._23 P1_kitP6_.1._23 P1_kitP6_.1._23

P1_kitP6_.1._23

P1_kitP6_.1._03 P1_kitP6_.1._03 P1_kitP6_.1._03

P1_kitP6_.1._03

P1_CD44high_01_10 P1_CD44high_01_10 P1_CD44high_01_10

P1_CD44high_01_10

P1_CD44high_01_18 P1_CD44high_01_18 P1_CD44high_01_18

P1_CD44high_01_18

P1_CD44high_01_02 P1_CD44high_01_02 P1_CD44high_01_02

P1_CD44high_01_02

P1_CD44high_01_16 P1_CD44high_01_16 P1_CD44high_01_16

P1_CD44high_01_16

P1_CD44low.C.kit._01_03.1 P1_CD44low.C.kit._01_03.1 P1_CD44low.C.kit._01_03.1

P1_CD44low.C.kit._01_03.1

P1_kitP6_.1._20 P1_kitP6_.1._20 P1_kitP6_.1._20

P1_kitP6_.1._20

P1_kitP6_.1._07 P1_kitP6_.1._07 P1_kitP6_.1._07

P1_kitP6_.1._07

P1_kitP6_.1._05 P1_kitP6_.1._05 P1_kitP6_.1._05

P1_kitP6_.1._05

P1_kitP6_.1._17 P1_kitP6_.1._17 P1_kitP6_.1._17

P1_kitP6_.1._17

P1_kitP6_.1._08 P1_kitP6_.1._08 P1_kitP6_.1._08

P1_kitP6_.1._08

P1_kitP6_.1._12 P1_kitP6_.1._12 P1_kitP6_.1._12

P1_kitP6_.1._12

P1_kitP6_.1._22 P1_kitP6_.1._22 P1_kitP6_.1._22

P1_kitP6_.1._22

P1_CD44high_01_08 P1_CD44high_01_08 P1_CD44high_01_08

P1_CD44high_01_08

P1_CD44high_01_01 P1_CD44high_01_01 P1_CD44high_01_01

P1_CD44high_01_01

P1_CD44high_01_07 P1_CD44high_01_07 P1_CD44high_01_07

P1_CD44high_01_07

P1_CD44high_01_09 P1_CD44high_01_09 P1_CD44high_01_09

P1_CD44high_01_09

P1_CD44high_01_19 P1_CD44high_01_19 P1_CD44high_01_19

P1_CD44high_01_19

P1_CD44high_01_11 P1_CD44high_01_11 P1_CD44high_01_11

P1_CD44high_01_11

P1_CD44high_01_20 P1_CD44high_01_20 P1_CD44high_01_20

P1_CD44high_01_20

P1_CD44low.C.kit._01_04 P1_CD44low.C.kit._01_04 P1_CD44low.C.kit._01_04

P1_CD44low.C.kit._01_04

P2_CD44low.C.kit._01_11.1 P2_CD44low.C.kit._01_11.1 P2_CD44low.C.kit._01_11.1

P2_CD44low.C.kit._01_11.1

P1_CD44high_01_13 P1_CD44high_01_13 P1_CD44high_01_13

P1_CD44high_01_13

P1_CD44high_01_15 P1_CD44high_01_15 P1_CD44high_01_15

P1_CD44high_01_15

P1_CD44high_01_03 P1_CD44high_01_03 P1_CD44high_01_03

P1_CD44high_01_03

P1_CD44high_01_04 P1_CD44high_01_04 P1_CD44high_01_04

P1_CD44high_01_04

P1_CD44high_01_21 P1_CD44high_01_21 P1_CD44high_01_21

P1_CD44high_01_21

P2_CD44low.C.kit._01_09.1 P2_CD44low.C.kit._01_09.1 P2_CD44low.C.kit._01_09.1

P2_CD44low.C.kit._01_09.1

P1_CD44high_01_06 P1_CD44high_01_06 P1_CD44high_01_06

P1_CD44high_01_06

P1_CD44high_01_12 P1_CD44high_01_12 P1_CD44high_01_12

P1_CD44high_01_12

P1_kitP6_.1._11 P1_kitP6_.1._11 P1_kitP6_.1._11

P1_kitP6_.1._11

P1_kitP6_.1._21 P1_kitP6_.1._21 P1_kitP6_.1._21

P1_kitP6_.1._21

P1_kitP7_.1._01 P1_kitP7_.1._01 P1_kitP7_.1._01

P1_kitP7_.1._01

P1_nokitP7_.1._09 P1_nokitP7_.1._09 P1_nokitP7_.1._09

P1_nokitP7_.1._09

P1_kitP6_.1._01 P1_kitP6_.1._01 P1_kitP6_.1._01

P1_kitP6_.1._01

P1_CD44low.C.kit._01_14 P1_CD44low.C.kit._01_14 P1_CD44low.C.kit._01_14

P1_CD44low.C.kit._01_14

P1_CD44low.C.kit._01_01.1 P1_CD44low.C.kit._01_01.1 P1_CD44low.C.kit._01_01.1

P1_CD44low.C.kit._01_01.1

P1_CD44low.C.kit._01_05.1 P1_CD44low.C.kit._01_05.1 P1_CD44low.C.kit._01_05.1

P1_CD44low.C.kit._01_05.1

P2_CD44low.C.kit._01_02.1 P2_CD44low.C.kit._01_02.1 P2_CD44low.C.kit._01_02.1

P2_CD44low.C.kit._01_02.1

P1_CD44low.C.kit._01_09.1 P1_CD44low.C.kit._01_09.1 P1_CD44low.C.kit._01_09.1

P1_CD44low.C.kit._01_09.1

P1_CD44high_01_05 P1_CD44high_01_05 P1_CD44high_01_05

P1_CD44high_01_05

P1_CD44high_01_24 P1_CD44high_01_24 P1_CD44high_01_24

P1_CD44high_01_24

P1_CD44high_01_22 P1_CD44high_01_22 P1_CD44high_01_22

P1_CD44high_01_22

P1_kitP6_.1._02 P1_kitP6_.1._02 P1_kitP6_.1._02

P1_kitP6_.1._02

P1_kitP7_.1._15 P1_kitP7_.1._15 P1_kitP7_.1._15

P1_kitP7_.1._15

P1_CD44low.C.kit._01_07.1 P1_CD44low.C.kit._01_07.1 P1_CD44low.C.kit._01_07.1

P1_CD44low.C.kit._01_07.1

P1_kitP6_.1._06 P1_kitP6_.1._06 P1_kitP6_.1._06

P1_kitP6_.1._06

P1_CD44low.C.kit._01_02.1 P1_CD44low.C.kit._01_02.1 P1_CD44low.C.kit._01_02.1

P1_CD44low.C.kit._01_02.1

P1_CD44low.C.kit._01_22.1 P1_CD44low.C.kit._01_22.1 P1_CD44low.C.kit._01_22.1

P1_CD44low.C.kit._01_22.1

P2_CD44low.C.kit._01_14.1 P2_CD44low.C.kit._01_14.1 P2_CD44low.C.kit._01_14.1

P2_CD44low.C.kit._01_14.1

P2_CD44low.C.kit._01_08.1 P2_CD44low.C.kit._01_08.1 P2_CD44low.C.kit._01_08.1

P2_CD44low.C.kit._01_08.1

P2_CD44low.C.kit._01_17.1 P2_CD44low.C.kit._01_17.1 P2_CD44low.C.kit._01_17.1

P2_CD44low.C.kit._01_17.1

P2_CD44low.C.kit._01_03.1 P2_CD44low.C.kit._01_03.1 P2_CD44low.C.kit._01_03.1

P2_CD44low.C.kit._01_03.1

P2_CD44low.C.kit._01_10.1 P2_CD44low.C.kit._01_10.1 P2_CD44low.C.kit._01_10.1

P2_CD44low.C.kit._01_10.1

P1_kitP7_.1._23 P1_kitP7_.1._23 P1_kitP7_.1._23

P1_kitP7_.1._23

P1_kitP6_.1._18 P1_kitP6_.1._18 P1_kitP6_.1._18

P1_kitP6_.1._18

P1_kitP7_.1._06 P1_kitP7_.1._06 P1_kitP7_.1._06

P1_kitP7_.1._06

P1_kitP7_.1._14 P1_kitP7_.1._14 P1_kitP7_.1._14

P1_kitP7_.1._14

P1_kitP7_.1._10 P1_kitP7_.1._10 P1_kitP7_.1._10

P1_kitP7_.1._10

P1_kitP7_.1._13 P1_kitP7_.1._13 P1_kitP7_.1._13

P1_kitP7_.1._13

P1_CD44low.C.kit._01_15.1 P1_CD44low.C.kit._01_15.1 P1_CD44low.C.kit._01_15.1

P1_CD44low.C.kit._01_15.1

P1_CD44high_01_14 P1_CD44high_01_14 P1_CD44high_01_14

P1_CD44high_01_14

P1_CD44low.C.kit._01_04.1 P1_CD44low.C.kit._01_04.1 P1_CD44low.C.kit._01_04.1

P1_CD44low.C.kit._01_04.1

P1_CD44low.C.kit._01_10.1 P1_CD44low.C.kit._01_10.1 P1_CD44low.C.kit._01_10.1

P1_CD44low.C.kit._01_10.1

P2_CD44low.C.kit._01_16 P2_CD44low.C.kit._01_16 P2_CD44low.C.kit._01_16

P2_CD44low.C.kit._01_16

P1_kitP7_.1._04 P1_kitP7_.1._04 P1_kitP7_.1._04

P1_kitP7_.1._04

P1_kitP7_.1._07 P1_kitP7_.1._07 P1_kitP7_.1._07

P1_kitP7_.1._07

P2_CD44low.C.kit._01_06.1 P2_CD44low.C.kit._01_06.1 P2_CD44low.C.kit._01_06.1

P2_CD44low.C.kit._01_06.1

P2_CD44low.C.kit._01_04.1 P2_CD44low.C.kit._01_04.1 P2_CD44low.C.kit._01_04.1

P2_CD44low.C.kit._01_04.1

P2_CD44low.C.kit._01_12.1 P2_CD44low.C.kit._01_12.1 P2_CD44low.C.kit._01_12.1

P2_CD44low.C.kit._01_12.1

P2_CD44low.C.kit._01_18 P2_CD44low.C.kit._01_18 P2_CD44low.C.kit._01_18

P2_CD44low.C.kit._01_18

P1_CD44low.C.kit._01_11.1 P1_CD44low.C.kit._01_11.1 P1_CD44low.C.kit._01_11.1

P1_CD44low.C.kit._01_11.1

P1_CD44low.C.kit._01_20.1 P1_CD44low.C.kit._01_20.1 P1_CD44low.C.kit._01_20.1

P1_CD44low.C.kit._01_20.1

P1_kitP7_.1._05 P1_kitP7_.1._05 P1_kitP7_.1._05

P1_kitP7_.1._05

P1_kitP7_.1._18 P1_kitP7_.1._18 P1_kitP7_.1._18

P1_kitP7_.1._18

P2_CD44low.C.kit._01_05.1 P2_CD44low.C.kit._01_05.1 P2_CD44low.C.kit._01_05.1

P2_CD44low.C.kit._01_05.1

P2_CD44low.C.kit._01_13.1 P2_CD44low.C.kit._01_13.1 P2_CD44low.C.kit._01_13.1 P2_CD44low.C.kit._01_13.1

P1_CD44low.C.kit._01_08.1 P1_CD44low.C.kit._01_08.1 P1_CD44low.C.kit._01_08.1 P1_CD44low.C.kit._01_08.1

P1_CD44low.C.kit._01_19.1 P1_CD44low.C.kit._01_19.1 P1_CD44low.C.kit._01_19.1

P1_CD44low.C.kit._01_19.1

P2_CD44low.C.kit._01_01.1 P2_CD44low.C.kit._01_01.1 P2_CD44low.C.kit._01_01.1 P2_CD44low.C.kit._01_01.1

P2_CD44low.C.kit._01_07.1 P2_CD44low.C.kit._01_07.1 P2_CD44low.C.kit._01_07.1 P2_CD44low.C.kit._01_07.1

P2_CD44low.C.kit._01_15.1 P2_CD44low.C.kit._01_15.1 P2_CD44low.C.kit._01_15.1 P2_CD44low.C.kit._01_15.1

P1_CD44low.C.kit._01_18.1 P1_CD44low.C.kit._01_18.1 P1_CD44low.C.kit._01_18.1 P1_CD44low.C.kit._01_18.1

P1_CD44low.C.kit._01_06.1 P1_CD44low.C.kit._01_06.1 P1_CD44low.C.kit._01_06.1 P1_CD44low.C.kit._01_06.1

P1_CD44low.C.kit._01_14.1 P1_CD44low.C.kit._01_14.1 P1_CD44low.C.kit._01_14.1 P1_CD44low.C.kit._01_14.1

P1_CD44low.C.kit._01_12.1 P1_CD44low.C.kit._01_12.1 P1_CD44low.C.kit._01_12.1 P1_CD44low.C.kit._01_12.1

P1_CD44low.C.kit._01_16.1 P1_CD44low.C.kit._01_16.1 P1_CD44low.C.kit._01_16.1 P1_CD44low.C.kit._01_16.1

P1_CD44low.C.kit._01_23.1 P1_CD44low.C.kit._01_23.1 P1_CD44low.C.kit._01_23.1 P1_CD44low.C.kit._01_23.1 Genotype Group ID Sox6 SERPINF1 ACTA2 ITGB3 Angpt1 Ikzf2 Ptk2b MYB ATP2A3 Plcg2 Med12l Mctp1 Stk26 Rab38 GFI1 Nfe2 ITGA2B Dnmt3b GATA2 Cbfb NOTCH1 CXCL12 Col3a1 Notch2 HMMR Lrp1 ITGAM Pag1 GFI1B EPOR HBB Mycn GATA1 SNAI1 BMP4 Pde2a PTPRB ESAM Sox17 ACVRL1 PCDH12 CDH2 GPR126 COL4A5 Pde3a FBN1 ERG Cbfa2t3 Pbx1 Bcl11a SMAD7 SMAD6 MEIS2 SMAD3 SMAD2 TAL1 Rbm38 KIT Adgrg1 EMB CD44 Laptm5 Pfas CD47 Gpx1 Nmt2 LMO2 TGFB1 FLI1 Dapp1 ALOX5AP SFPI1 CORO1A PLA2G4A Was RUNX1 Dok2 Ptpn6 SLA Ikzf1 Dock2 LGALS9 PTPRC Csf1r Ly6e Stxbp2 Dock8 PECAM1 Mdk CDH5 TEK COL4A2 RAMP2 KDR Emcn PPIA GroupID Sox6 SERPINF1 ACTA2 ITGB3 Angpt1 Ikzf2 Ptk2b MYB ATP2A3 Plcg2 Med12l Mctp1 Stk26 Rab38 GFI1 Nfe2 ITGA2B Dnmt3b GATA2 Cbfb NOTCH1 CXCL12 Col3a1 Notch2 HMMR Lrp1 ITGAM Pag1 GFI1B EPOR HBB Mycn GATA1 SNAI1 BMP4 Pde2a PTPRB ESAM Sox17 ACVRL1 PCDH12 CDH2 GPR126 COL4A5 Pde3a FBN1 ERG Cbfa2t3 Pbx1 Bcl11a SMAD7 SMAD6 MEIS2 SMAD3 SMAD2 TAL1 Rbm38 KIT Adgrg1 EMB CD44 Laptm5 Pfas CD47 Gpx1 Nmt2 LMO2 TGFB1 FLI1 Dapp1 ALOX5AP SFPI1 CORO1A PLA2G4A Was RUNX1 Dok2 Ptpn6 SLA Ikzf1 Dock2 LGALS9 PTPRC Csf1r Ly6e Stxbp2 Dock8 PECAM1 Mdk CDH5 TEK COL4A2 RAMP2 KDR Emcn PPIA GroupID Sox6 SERPINF1 ACTA2 ITGB3 Angpt1 Ikzf2 Ptk2b MYB ATP2A3 Plcg2 Med12l Mctp1 Stk26 Rab38 GFI1 Nfe2 ITGA2B Dnmt3b GATA2 Cbfb NOTCH1 CXCL12 Col3a1 Notch2 HMMR Lrp1 ITGAM Pag1 GFI1B EPOR HBB Mycn GATA1 SNAI1 BMP4 Pde2a PTPRB ESAM Sox17 ACVRL1 PCDH12 CDH2 GPR126 COL4A5 Pde3a FBN1 ERG Cbfa2t3 Pbx1 Bcl11a SMAD7 SMAD6 MEIS2 SMAD3 SMAD2 TAL1 Rbm38 KIT Adgrg1 EMB CD44 Laptm5 Pfas CD47 Gpx1 Nmt2 LMO2 TGFB1 FLI1 Dapp1 ALOX5AP SFPI1 CORO1A PLA2G4A Was RUNX1 Dok2 Ptpn6 SLA Ikzf1 Dock2 LGALS9 PTPRC Csf1r Ly6e Stxbp2 Dock8 PECAM1 Mdk CDH5 TEK COL4A2 RAMP2 KDR Emcn PPIA GroupID Sox6 SERPINF1 ACTA2 ITGB3 Angpt1 Ikzf2 Ptk2b MYB ATP2A3 Plcg2 Med12l Mctp1 Stk26 Rab38 GFI1 Nfe2 ITGA2B Dnmt3b GATA2 Cbfb NOTCH1 CXCL12 Col3a1 Notch2 HMMR Lrp1 ITGAM Pag1 GFI1B EPOR HBB Mycn GATA1 SNAI1 BMP4 Pde2a PTPRB ESAM Sox17 ACVRL1 PCDH12 CDH2 GPR126 COL4A5 Pde3a FBN1 ERG Cbfa2t3 Pbx1 Bcl11a SMAD7 SMAD6 MEIS2 SMAD3 SMAD2 TAL1 Rbm38 KIT Adgrg1 EMB CD44 Laptm5 Pfas CD47 Gpx1 Nmt2 LMO2 TGFB1 FLI1 Dapp1 ALOX5AP SFPI1 CORO1A PLA2G4A Was RUNX1 Dok2 Ptpn6 SLA Ikzf1 Dock2 LGALS9 PTPRC Csf1r Ly6e Stxbp2 Dock8 PECAM1 Mdk CDH5 TEK COL4A2 RAMP2 KDR Emcn PPIA GroupID − − − − BH1 BH1 BH1 BH1 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 GroupID GroupID GroupID GroupID SC_5 SC_4 SC_3 SC_2 SC_1 Rx1ko WT Rx1ko WT Rx1ko_CD44neg Rx1ko_44lo_Kitpos Rx1ko_44lo_Kitneg SC_5 SC_4 SC_3 SC_2 SC_1 Rx1ko_CD44neg Rx1ko_44lo_Kitpos Rx1ko_44lo_Kitneg Supplementary Figure S5: Results of single cell q-RT-PCR analysis in the wild type and Runx1-/- AGM Single cells from Runx1-/- CD44Neg, Runx1-/- CD44LowKitNeg and Runx1-/- CD44LowKitPos populations were isolated and tested by single-cell q-RT-PCR. The heatmap shows the result of the hierarchical clustering analysis in combination with the wild type single-cells from Figure 3a (cells were clustered by Euclidian distance and the genes by Pearson correlation). See also Figure 7 and Supplementary File S7.

Oatley, Vargel-Bölükbası et al. 2018 Supplementary Figure S6: Time course of CD44 expression during Haemangioblast culture

Blast Day 1 Blast Day 2 Blast Day 3 12.2% 6.16% 5 4.7% 17.8% 5 22.9% 5 17.8% 10 10 10

4 4 4 10 10 10 Cad -

3 3 3 10 10 10 VE

0 0 0

3 3 3 -10 7.1% 70.4% -10 10.4% 54.5% -10 18.1% 57.9%

3 4 5 3 4 5 3 4 5 0 10 10 10 0 10 10 10 0 10 10 10

CD44

Oatley, Vargel-Bölükbası et al. 2018 Supplementary Figure S6: Time course of CD44 expression during haemangioblast culture Flow cytometry analysis of CD44 expression in Haemangioblast culture between day 1 and day 3. The dot plots show expression of VE-Cadherin and CD44 at the indicated time points. See also Figure 9.

Oatley, Vargel-Bölükbası et al. 2018 Description of the supplementary files and tables

Supplementary File S1: Expression Data from single-cell RNA-seq from Fig. 1d

The first worksheet contains log10(1+TPM) expression data from single-cell RNA-seq and the second the metadata relative to the cells shown in Fig. 1d.

Supplementary File S2: Results of single-cell q-RT-PCR from Fig. S2 The first worksheet contains log2 expression data from single-cell q-RT-PCR and the second the metadata relative to the cells shown in Fig. S2.

Supplementary File S3: Results of single-cell q-RT-PCR from Fig. 3 The first worksheet contains log2 expression data from single-cell q-RT-PCR and the second the metadata relative to the cells shown in Fig. 3.

Supplementary File S4: Results of single-cell q-RT-PCR from Fig. S3 The first worksheet contains log2 expression data from single-cell q-RT-PCR and the second the metadata relative to the cells shown in Fig. S3.

Supplementary File S5: Results of the RNA sequencing from Fig. 5 First worksheet: Matrix showing rlog transformed expression values after normalization with the DSEQ2 package. Second worksheet: Metadata related to the samples in Fig.5 Third worksheet: Gene list resulting from the differential expression analysis between the CD44Neg and CD44LowKitNeg populations (p-value_adjusted <0.01). The results were obtained following the Wald statistical test. Negative LogFC values indicate higher gene expression in CD44LowKitNeg compared to CD44Neg while positive LogFC values indicate higher expression in CD44Neg compared to CD44LowKitNeg. Third worksheet: Expression matrix used in Fig. 5b. Fourth worksheet: Expression matrix used in Fig. 5c. Fifth worksheet: Expression matrix used in Fig. 5d.

Oatley, Vargel-Bölükbası et al. 2018 Supplementary File S6: Expression Data from single-cell RNA-seq from Fig. S4

The first worksheet contains log10(1+TPM) expression data from single-cell RNA-seq and the second the metadata relative to the cells shown in Fig. 1d.

Supplementary File S7: Results of single-cell q-RT-PCR from Fig. S5 The first worksheet contains log2 expression data from single-cell q-RT-PCR and the second the metadata relative to the cells shown in Fig. S5.

Supplementary Table S1: Results of the antibody screen List of the forty-two antigens (out of 176) expressed by VE-Cad+ cells from day 1.5 haemangioblast culture following the antibody screen. Sixteen of these markers have a bimodal expression (indicated in bold). See also Supplementary Figure S1.

Supplementary Table S2: List of primers for single-cell q-RT-PCR These primers were used to detect the genes shown in Fig. 3, Fig. S3 and Fig. S5.

Supplementary Table S3: Results of the reporter metabolite analysis from Fig. 6 Table listing the results of the reporter metabolite analysis generated from the comparison of differentially expression genes between CD44Neg and CD44LowKitNeg populations.

Oatley, Vargel-Bölükbası et al. 2018