Agilent Infinitylab LC Series 1260 Infinity II Binary Pump User Manual
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Agilent InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Agilent Technologies Notices © Agilent Technologies, Inc. 2016-2018 Warranty receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June No part of this manual may be reproduced The material contained in this docu- in any form or by any means (including 1987). U.S. Government users will receive ment is provided “as is,” and is sub- no greater than Limited Rights as defined in electronic storage and retrieval or transla- ject to being changed, without notice, FAR 52.227-14 (June 1987) or DFAR tion into a foreign language) without prior agreement and written consent from Agi- in future editions. 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This manual covers the Agilent InfinityLab LC Series 1260 Infinity II Binary Pump (G7112B). 1Introduction This chapter gives an introduction to the module and an instrument overview. 2 Site Requirements and Specifications This chapter provides information about site requirements and specifications for the binary pump. 3Using the Pump This chapter explains the operational parameters of the Binary Pump. 4 Optimizing Performance This chapter gives information on how to optimize the performance of the Binary Pump under special operational conditions. 5 Troubleshooting and Diagnostics Overview of the troubleshooting and diagnostic features. 6 Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. 7 Test Functions and Calibration This chapter explains all test functions that are available for the binary pump. 8 Maintenance This chapter describes the maintenance of the module. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 3 In This Guide... 9 Parts and Materials for Maintenance This chapter lists all parts and tools that are required for maintenance and simple repairs. 10 Identifying Cables This chapter provides information on cables used with the Agilent InfinityLab LC Series modules. 11 Hardware Information This chapter provides detailed technical information about your binary pump. 12 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC. 13 Appendix This chapter provides addition information on safety, legal and web. 4 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Contents Contents 1Introduction9 Instrument and Operation 10 Overview of the Hydraulic Path 15 Leak and Waste Handling 20 2 Site Requirements and Specifications 25 Site Requirements 26 Physical Specifications 29 Performance Specifications 30 3Using the Pump33 Magnets 34 Turn on/off 35 Status Indicators 37 Best Practices 38 Leak and Waste Handling 40 Hints for Successful Use of the Binary Pump 41 Setting up the Pump with the G4208A Instant Pilot 42 Setting up the Pump with the Instrument Control Interface 43 Solvent Information 48 Algae Growth in HPLC Systems 54 Prevent Blocking of Solvent Filters 55 Normal Phase Applications 57 4 Optimizing Performance 59 When to Use a Vacuum Degasser 60 When to Use the Active Seal Wash Option 61 When to Use the Low Volume Mixer 62 When to Remove Damper and Mixer 63 How to Optimize the Compressibility Compensation Setting 66 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 5 Contents 5 Troubleshooting and Diagnostics 69 Overview of the Module’s Indicators and Test Functions 70 User Interfaces 72 Agilent Lab Advisor Software 73 6 Error Information 75 What Are Error Messages 77 General Error Messages 78 Module Error Messages 84 7 Test Functions and Calibration 95 (System) Pressure Test 96 Valve Test 101 Binary Pump Solvent Compressibility Calibration 103 Pump Elasticity Calibration 105 Pump Leak Rate Test 107 8 Maintenance 111 Introduction to Maintenance 112 Cautions and Warnings 113 Cleaning the Module 115 Overview of Maintenance and Simple Repairs 116 Maintenance Procedures 117 Remove and Install Doors 118 Exchange the Purge Valve Frit or the Purge Valve 120 Replace the O-Ring on the Purge Valve 123 Remove the Pump Head Assembly 125 Maintenance of a Pump Head without Seal Wash 127 Maintenance of a Pump Head with Seal Wash 130 Reinstall the Pump Head Assembly 134 Seal Wear-in Procedure 136 Exchange the Active Inlet Valve (AIV) or its Cartridge 137 Exchange the Seal Wash Cartridge 140 Replace Leak Handling System Parts 142 Exchange the Outlet Valve 144 Installation of the Solvent Selection Valve Upgrade Kit 146 Exchange the Solvent Selection Valve 148 Replacing Module Firmware 151 6 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Contents 9 Parts and Materials for Maintenance 153 Hydraulic Path with Solvent Selection Valve 154 Hydraulic Path without Solvent Selection Valve 156 Pump Head Assembly Without Seal Wash 158 Pump Head Assembly with Seal Wash Option 160 Outlet Valve 162 Purge Valve Assembly 163 Active Inlet Valve Assembly 164 HPLC System Tool Kit 165 Active Seal Wash Option 166 Solvent Cabinet 167 Bottle Head Assembly 168 Cover Parts 169 Accessory Kit 170 10 Identifying Cables 171 Cable Overview 172 Analog Cables 174 Remote Cables 176 CAN/LAN Cables 180 RS-232 Cable Kit 181 Agilent 1200 Module to Printer 182 11 Hardware Information 183 Firmware Description 184 Electrical Connections 187 Interfaces 189 Setting the 6-bit Configuration Switch 197 Early Maintenance Feedback 201 Instrument Layout 202 12 LAN Configuration 203 What You Have to Do First 204 TCP/IP parameter configuration 205 Configuration Switches 206 Initialization Mode Selection 207 Dynamic Host Configuration Protocol (DHCP) 209 Manual Configuration 212 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 7 Contents 13 Appendix 217 General Safety Information 218 Waste Electrical and Electronic Equipment (WEE) Directive 224 Radio Interference 225 Sound Emission 226 Agilent Technologies on the Internet 227 8 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 1 Introduction Instrument and Operation 10 Introduction to the Pump 10 Principle of Operation 11 Product Description 14 Features 14 Overview of the Hydraulic Path 15 Leak and Waste Handling 20 Leak Sensor 23 Waste Concept 24 This chapter gives an introduction to the module and an instrument overview. Agilent Technologies 9 1 Introduction Instrument and Operation Instrument and Operation Introduction to the Pump The binary pump comprises two identical pumps integrated into one housing. Binary gradients are created by high-pressure mixing. A built-in degasser is available for applications that require best flow stability, especially at low flow rates, for maximum detector sensitivity. Pulse damper and mixer can be bypassed for low flowrate applications or whenever a minimal transient volume is desirable. Typical applications are high throughput methods with fast gradients on high resolution 2.1 mm columns. The pump is capable of delivering flow in the range of 0.1 – 5 mL/min against up to 600 bar. A solvent selection valve (optional) allows to form binary mixtures (isocratic or gradient) from one of two solvents per channel. Active seal wash (optional) is available for use with concentrated buffer solutions. Figure 1 Overview of the binary pump 10 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Introduction 1 Instrument and Operation Principle of Operation The binary pump is based on a two-channel, dual-piston in-series design which comprises all essential functions that a solvent delivery system has to fulfill.