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Fluorolog®-3 with Fluoressence™ Fluorolog-3 Operation Manual rev. G (2 May 2014) Fluorolog®-3 with FluorEssence™ Operation Manual www.HORIBA.com/scientific rev. G i Fluorolog-3 Operation Manual rev. G (2 May 2014) Copyright © 2002–2014 by HORIBA Instruments Incorporated. All rights reserved. No part of this work may be reproduced, stored, in a retrieval system, or transmitted in any form by any means, including electronic or mechanical, photocopying and recording, without prior written permission from HORIBA Instruments Incorporated. Requests for permission should be requested in writing. Origin® is a registered trademark of OriginLab Corporation. Alconox® is a registered trademark of Alconox, Inc. Ludox® is a registered trademark of W.R. Grace and Co. Teflon® is a registered trademark of E.I. du Pont de Nemours and Company. Windows® and Excel® are registered trademarks of Microsoft Corporation. Information in this manual is subject to change without notice, and does not represent a commitment on the part of the vendor. May 2014 Part number J81014 ii Fluorolog-3 Operation Manual rev. G (2 May 2014) Table of Contents 0: Introduction ................................................................................................. 0-1 About the Fluorolog®-3 ........................................................................................................................... 0-1 Chapter overview .................................................................................................................................... 0-2 Disclaimer ............................................................................................................................................... 0-4 Safety summary ...................................................................................................................................... 0-6 Risks of ultraviolet exposure ................................................................................................................... 0-9 Additional risks of xenon lamps ............................................................................................................ 0-11 1: Requirements & Installation ............................................................................. 1-1 Surface requirements ............................................................................................................................ 1-1 Environmental requirements. .................................................................................................................. 1-2 Electrical requirements .......................................................................................................................... 1-3 Installation ............................................................................................................................................... 1-4 2: System Description ....................................................................................... 2-1 Overview ................................................................................................................................................. 2-1 Configurations ......................................................................................................................................... 2-3 3: System Operation ......................................................................................... 3-1 Turning on the system ............................................................................................................................ 3-1 Checking system performance ............................................................................................................... 3-3 Useful materials for characterizing system and samples ..................................................................... 3-16 4: Data Acquisition ............................................................................................ 4-1 Introduction ............................................................................................................................................. 4-1 Experiment Menu button......................................................................................................................... 4-2 Previous Experiment Setup button ......................................................................................................... 4-4 Auto Run Previous Experiment button ................................................................................................... 4-5 Make Overlay File button ........................................................................................................................ 4-6 3D Scan to 3D Profile button ................................................................................................................. 4-7 Run JY Batch Experiments button .......................................................................................................... 4-9 Real Time Control button ...................................................................................................................... 4-11 Create/Use Calibration Curve from CWA Data button ......................................................................... 4-12 2D Intensity Map button ........................................................................................................................ 4-16 Launch DataStation button ................................................................................................................... 4-17 Running an unknown sample ............................................................................................................... 4-18 Using corrected signals in FluorEssence™ .......................................................................................... 4-23 5: Optimizing Data ........................................................................................... 5-1 Cuvette preparation ............................................................................................................................... 5-1 Sample preparation ............................................................................................................................... 5-2 Running a scan on a sample .................................................................................................................. 5-4 Data collection techniques ...................................................................................................................... 5-6 Correcting data ..................................................................................................................................... 5-12 6: System Maintenance ..................................................................................... 6-1 External Case ........................................................................................................................................ 6-1 Lamp-housing exhaust filters .................................................................................................................. 6-1 Xenon lamp ............................................................................................................................................. 6-2 Installing an optional new photomultiplier tube ..................................................................................... 6-17 Reference signal detector ..................................................................................................................... 6-18 Gratings ................................................................................................................................................ 6-19 Mirrors ................................................................................................................................................... 6-22 Automated 4-position turret .................................................................................................................. 6-23 iii Fluorolog-3 Operation Manual rev. G (2 May 2014) 7: Troubleshooting ............................................................................................ 7-1 Chart ....................................................................................................................................................... 7-1 Using diagnostic spectra ....................................................................................................................... 7-3 Further assistance .................................................................................................................................. 7-8 8: Producing Correction Factors ........................................................................... 8-1 Introduction ............................................................................................................................................. 8-1 Types of Correction-Factor Kits .............................................................................................................. 8-2 Generating emission correction factors with the F-3026 Correction Factor Kit ...................................... 8-3 Calculating emission correction factors .................................................................................................. 8-8 Calculating excitation correction factors ................................................................................................. 8-9 Using correction-factor files .................................................................................................................
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