agronomy Article Screening of the Candidate DNA Barcodes for Three Important Amorphophallus Species Identification 1, 1, 1,2 3 1 1 Chufeng Zhao y , Xuan She y, Erxi Liu , Nunung Harijati , Teng Cheng , Zhongli Hu , Surong Jin 4,* and Ying Diao 1,* 1 State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China;
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[email protected] (Z.H.) 2 Institute of Konjac, Enshi Academy of Agricultural Sciences, Enshi 445000, China 3 Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl.Veteran Malang 65145, Indonesia;
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[email protected] (Y.D.); Tel.: +86-27-13971209084 (S.J.); +86-27-68753606 (Y.D.) Authors contribute equally to this work. y Received: 18 August 2020; Accepted: 7 September 2020; Published: 11 September 2020 Abstract: Amorphophallus is widely distributed in Southeast Asia, Africa, and other places, with more than 170 species. Amorphophallus has high medicinal value and is commonly used in medicine. However, the current classification based on morphology is challenging in with regard to Amorphophallus and closely related species. This study used six barcodes, namely ITS2, matK, rcbL, nad1, trnH-psbA, and trnL-trnF, to evaluate their identification ability for three important Amorphophallus species, including A. konjac, A. albus, and A. muelleri. We recommend that trnH-psbA can be applied to the Amorphophallus trade, quickly identify the purity of A.