DOCSLIB.ORG

Lucidascocarpa Pulchella, a New Ascomycete Genus and Species from Freshwater Habitats in the American Tropics

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Lucidascocarpa Pulchella, a New Ascomycete Genus and Species from Freshwater Habitats in the American Tropics Mycologia, 100(4), 2008, pp. 642–646. DOI: 10.3852/07-152R2 # 2008 by The Mycological Society of America, Lawrence, KS 66044-8897 Lucidascocarpa pulchella, a new ascomycete genus and species from freshwater habitats in the American tropics Astrid Ferrer1 species in this family. We therefore describe this Huzefa A. Raja fungus as a new genus and species in the Dothidea- Carol A. Shearer ceae. Department of Plant Biology, University of Illinois, Room 265 Morrill Hall, 505 South Goodwin Avenue, Urbana, Illinois 61801 MATERIALS AND METHODS Collection techniques and fungal isolation methods are presented in Ferrer and Shearer (2005). Ascomata on Abstract: A new fungus collected from submerged wood were removed and crushed in a drop of distilled wood in Costa Rica and Ecuador has ascostromatic water on a glass slide. One percent aqueous nigrosin was ascomata with fissitunicate asci and lacks pseudopar- added to the aqueous mounts to reveal gelatinous sheaths. aphyses, characters that place it in the Dothideaceae Measurements of the asci and ascospores were made of (Dothideales). It is unusual in the order because it material mounted in distilled water or material fixed in has white ascomata. Based on other morphological glycerin or lactic acid. Slides were preserved with the double cover glass method of Volkmann-Kohlmeyer and Kohlmeyer characters however this fungus could not be accom- (1996). Digital images were shot with a Spot RT digital modated in any existing genus in the Dothideaceae camera with an Olympus microscope equipped with and it is described herein as a new genus and species, Nomarski interference optics. Images were edited in Lucidascocarpa pulchella. These morphological fea- Photoshop CS2. Specimens were deposited in the Herbar- tures are characteristic of L. pulchella:ascomata ium of the University of Illinois at Urbana-Champaign glistening, white, each with a long, periphysate neck; (ILL). a membranous peridium composed of 5–7 thin- walled, hyaline cells; pseudoparaphyses absent; asci TAXONOMY fissitunicate, clavate, eight-spored; ascospores seven- septate, hyaline, multiguttulate, verruculose, sur- Lucidascocarpa A. Ferrer, Raja & Shearer, gen. nov. rounded by a large, regular, gelatinous sheath. Type species: Lucidascocarpa pulchella A. Ferrer, Raja & Key words: aquatic fungi, ascomycetes, Dothi- Shearer deales, Dothideomycetes, fungi, submerged woody Etymology: From Latin Lucido 5 shining; ascocarpa 5 debris, systematics ascocarp Ascomata albea, superficialis vel immersa, solitaria vel gregaria, globosa vel subglobosa, ostiolata. Peridium mem- INTRODUCTION branaceum. Asci fissitunicati, clavati, pedicellati, octospori, During a latitudinal survey of freshwater ascomy- biseriati. Pseudoparaphyses absentia. Ascosporae ellipsoi- cetes in Central and South America an unusual deae-fusiformes, hyalinae, septatae, guttulatae, verruculo- ascomycete species was found on woody debris sae, circumcinctae vagina gelatinosa. submerged in streams. This species can be placed Ascomata white, superficial or immersed, scat- within the Dothideaceae, Dothideales, a family of tered or clustered, venter globose to subglobose. ascostromatic fungi that have fissitunicate asci but Neck long, cylindrical, white, periphysate. Peridium that lack pseudoparaphyses (Barr 1987, Eriksson membranous, composed of 5–7 elongated, thin- 2006, Rossman 1987). This new species shares the walled, hyaline cells; tissue of textura angularis in diagnostic morphological characteristics of several surface view. Pseudoparaphyses absent. Asci fissituni- genera in the Dothideaceae but is distinct from other cate, clavate, pedicellate, with gelatinous material around the apex of the endoascus, containing Accepted for publication 29 April 2008. eight overlapping, biseriate ascospores. Ascospores 1 Corresponding author. E-mail: [email protected] ellipsoidal to fusiform, seven-septate, hyaline, multi- R FIGS. 1–10. Lucidascocarpa pulchella from the holotype. 1. Hyaline ascomata on surface of wood. 2. Neck with hyaline hyphae. 3. Peridium of an ascoma. 4. One ascoma in optical section showing discharge of ascospores through the neck. 5. Clavate ascus. 6. Ascus stained with 1% nigrosin showing the staining reaction of the ascospore end cells. 7. Ascus fixed with 642 FERRER ET AL: LUCIDASCOCARPA PULCHELLA GEN ET SP. NOV. 643 lactic acid. Arrows indicating the gelatinous material between the ecto- and endoascus at ascus apex. 8. Fissitunicate ascus dehiscence. 9. Partially empty ascus with arrows indicating the gelatinous material surrounding the apex of the endoascus. 10. Released ascus with arrows indicating the inward curling of the ectoascus wall. Bars: 1 5 500 mm; 2, 3 5 10 mm; 4, 5, 7, 8, 9, 10 5 10 mm; 6 5 20 mm. 644 MYCOLOGIA guttulate, verruculose, surrounded by a large gelati- Additional specimens examined: COSTA RICA. ALA- nous sheath. JUELA: Can˜o Negro Reserve, Frio Stream, on submerged, decorticated wood, 10u539N, 84u459W, water 28 C, pH 5, 15 Dec 2005, A. Ferrer & M. Salazar, AF280-1. (ILL 40555); Lucidascocarpa pulchella A. Ferrer, Raja & Shearer, LIMON: Barra del Colorado and Tortuguero National Park, sp. nov. Las Palmas stream, on submerged, decorticated wood, Ascomata 105–200 3 90–180 mm, solitaria vel gre- 10u359N, 83u319W, water 25 C, pH 5, 18 Dec 2005, A. Ferrer garia, superficialis vel immersa, albidus, globosa vel sub- & M. Salazar, AF280-2. (ILL 40556); HEREDIA: La Selva, globosa, rostrata, membranacea. Rostrum 160–280 3 38– Sarapiqui River, on submerged, decorticated wood, 50 mm, cylindraceum, periphysatum, albidum. Peridium 10– 10u269N, 84u019W, water 25 C, pH 5, 10 Jan 2006, M. 14 mm crassum. Asci 145–150 3 28–30 mm, fissitunicati, Salazar, AF280-5. (ILL 40557). clavati, pedicellati, octospori, biseriati. Pseudoparaphyses absentia. Ascosporae 36–50 3 12–16 mm, 7–septatae, DISCUSSION hyalinae, ellipsoideae-fusiformes, multiguttulatae, verrucu- losae, circumcinctae vagina gelatinosa, grandia, extensa in Noteworthy features of Lucidascocarpa pulchella aqua. include the white, glistening ascomata with long Ascomata on wood, scattered to aggregated, nu- necks (FIGS. 1–2) that are easily seen on the substra- merous, superficial to partially immersed with white, tum, the fissitunicate ascus in which the endoascus glistening necks protruding from the substrate, venter apex is covered with gelatinous material (FIGS.7–9) globose to subglobose, 105–200 3 90–180 mm diam, and the hyaline, phragmoseptate, rough-walled asco- white, glistening (FIG. 1). Neck 160–280 3 38–50 mm, spores (FIG. 15) that have guttules in a dictyose periphysate, cylindrical, curving irregularly, short pattern (FIGS. 11, 12). Apical cells of the ascospores hyphae protruding from the surface of the neck have fewer guttules than the other cells of the (FIGS. 1, 2, 4). Peridium 10–14 mm wide, composed of ascospore (FIGS. 11, 12) and they stain darkly in 5–7 elongated, thin-walled, hyaline cells (FIG.3), nigrosin (FIGS. 5, 6). The ascospore sheath maintains tissue of textura angularis in surface view. Pseudopar- a regular edge and shape as it enlarges in water aphyses absent. Asci 145–150 3 28–30 mm (mean 5 (FIGS. 11, 12, 14) and is difficult to observe once 144 3 29 mm, n 5 20), clavate, pedicellate, expanded. The sheath of ascospores mounted directly straight or at times slightly curved toward the base on lactic acid or nigrosin remains unexpanded (FIGS. 5, 6), with gelatinous material deposited (FIGS. 13, 15). Some sheaths that were ruptured around the apex of endoascus (FIGS. 7, 9), containing appeared to adhere to other materials in the slide 8 overlapping, obliquely biseriate ascospores (FIGS.5, preparation by sticky material within the sheath. This 6). Dehiscence fissitunicate, ectotunica rupturing at feature could help the spores attach to substrata in the apex and becoming strongly wrinkled and curled flowing water. inwards, endotunica elongating to a quarter length of Among the Dothideomycetes with light colored the ascus (FIGS. 8, 9, 10). Ascospores 36–50 3 12– ascocarps Lucidascocarpa is similar to Aliquandostipite 16 mm (mean 5 40 3 13 mm, n 5 50), seven-septate, Inderb., a genus in the Jahnulales (Pang et al 2002) in hyaline, ellipsoidal to fusiform, verruculose (FIGS. 11– having translucent, light-colored fruiting bodies and 13, 15), multiguttulate in a dictyose pattern (FIGS. 11, septate ascospores surrounded by a broad gelatinous 12), end cells lacking guttules, staining blue in sheath (Inderbitzin et al 2001). However Lucidasco- aqueous nigrosin (FIGS. 6, 11–13), surrounded by a carpa does not have pseudoparaphyses or the wide, large gelatinous sheath; gelatinous sheath ca. 2 mm brown, septate hyphae that characterize the Jahnu- wide in ascus (FIGS. 8, 9, 11), expanding in water to lales (Campbell et al 2007). 28–60 mm wide at the mid region, 68–128 mm long at Lucidascocarpa can be accommodated in the ascospore apices (FIG. 14), germinating from asco- Dothideales (sensu Barr 1987) because it has spore apices (FIG. 16). Colonies on potato dextrose fissitunicate asci and lacks pseudoparaphyses. It is agar (Difco), slow growing, 0.5 cm diam in 4 wk at unusual in the order because of its white ascomata. 24 C, dark brown, compact, with little aerial myceli- Among the Dothideales Lucidascocarpa shows some um. Anamorph not observed. similarities to the genus Hyalocrea H. Sydow & Sydow Known distribution: Costa Rica and Ecuador. in the Dothideaceae (Rossman 1987), in having light Etymology: From Latin Pulchella 5 very pretty. colored ascomata. However the ascomata
Load more

Recommended publications
  • Old Woman Creek National Estuarine Research Reserve Management Plan 2011-2016
    Old Woman Creek National Estuarine Research Reserve Management Plan 2011-2016 April 1981 Revised, May 1982 2nd revision, April 1983 3rd revision, December 1999 4th revision, May 2011 Prepared for U.S. Department of Commerce Ohio Department of Natural Resources National Oceanic and Atmospheric Administration Division of Wildlife Office of Ocean and Coastal Resource Management 2045 Morse Road, Bldg. G Estuarine Reserves Division Columbus, Ohio 1305 East West Highway 43229-6693 Silver Spring, MD 20910 This management plan has been developed in accordance with NOAA regulations, including all provisions for public involvement. It is consistent with the congressional intent of Section 315 of the Coastal Zone Management Act of 1972, as amended, and the provisions of the Ohio Coastal Management Program. OWC NERR Management Plan, 2011 - 2016 Acknowledgements This management plan was prepared by the staff and Advisory Council of the Old Woman Creek National Estuarine Research Reserve (OWC NERR), in collaboration with the Ohio Department of Natural Resources-Division of Wildlife. Participants in the planning process included: Manager, Frank Lopez; Research Coordinator, Dr. David Klarer; Coastal Training Program Coordinator, Heather Elmer; Education Coordinator, Ann Keefe; Education Specialist Phoebe Van Zoest; and Office Assistant, Gloria Pasterak. Other Reserve staff including Dick Boyer and Marje Bernhardt contributed their expertise to numerous planning meetings. The Reserve is grateful for the input and recommendations provided by members of the Old Woman Creek NERR Advisory Council. The Reserve is appreciative of the review, guidance, and council of Division of Wildlife Executive Administrator Dave Scott and the mapping expertise of Keith Lott and the late Steve Barry.
    [Show full text]
  • The Taxonomy, Phylogeny and Impact of Mycosphaerella Species on Eucalypts in South-Western Australia
    The Taxonomy, Phylogeny and Impact of Mycosphaerella species on Eucalypts in South-Western Australia By Aaron Maxwell BSc (Hons) Murdoch University Thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy School of Biological Sciences and Biotechnology Murdoch University Perth, Western Australia April 2004 Declaration I declare that the work in this thesis is of my own research, except where reference is made, and has not previously been submitted for a degree at any institution Aaron Maxwell April 2004 II Acknowledgements This work forms part of a PhD project, which is funded by an Australian Postgraduate Award (Industry) grant. Integrated Tree Cropping Pty is the industry partner involved and their financial and in kind support is gratefully received. I am indebted to my supervisors Associate Professor Bernie Dell and Dr Giles Hardy for their advice and inspiration. Also, Professor Mike Wingfield for his generosity in funding and supporting my research visit to South Africa. Dr Hardy played a great role in getting me started on this road and I cannot thank him enough for opening my eyes to the wonders of mycology and plant pathology. Professor Dell’s great wit has been a welcome addition to his wealth of knowledge. A long list of people, have helped me along the way. I thank Sarah Jackson for reviewing chapters and papers, and for extensive help with lab work and the thinking through of vexing issues. Tania Jackson for lab, field, accommodation and writing expertise. Kar-Chun Tan helped greatly with the RAPD’s research. Chris Dunne and Sarah Collins for writing advice.
    [Show full text]
  • Molecular Systematics of the Marine Dothideomycetes
    available online at www.studiesinmycology.org StudieS in Mycology 64: 155–173. 2009. doi:10.3114/sim.2009.64.09 Molecular systematics of the marine Dothideomycetes S. Suetrong1, 2, C.L. Schoch3, J.W. Spatafora4, J. Kohlmeyer5, B. Volkmann-Kohlmeyer5, J. Sakayaroj2, S. Phongpaichit1, K. Tanaka6, K. Hirayama6 and E.B.G. Jones2* 1Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand; 2Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Paholyothin Road, Khlong 1, Khlong Luang, Pathum Thani, 12120, Thailand; 3National Center for Biothechnology Information, National Library of Medicine, National Institutes of Health, 45 Center Drive, MSC 6510, Bethesda, Maryland 20892-6510, U.S.A.; 4Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, 97331, U.S.A.; 5Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina 28557, U.S.A.; 6Faculty of Agriculture & Life Sciences, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan *Correspondence: E.B. Gareth Jones, [email protected] Abstract: Phylogenetic analyses of four nuclear genes, namely the large and small subunits of the nuclear ribosomal RNA, transcription elongation factor 1-alpha and the second largest RNA polymerase II subunit, established that the ecological group of marine bitunicate ascomycetes has representatives in the orders Capnodiales, Hysteriales, Jahnulales, Mytilinidiales, Patellariales and Pleosporales. Most of the fungi sequenced were intertidal mangrove taxa and belong to members of 12 families in the Pleosporales: Aigialaceae, Didymellaceae, Leptosphaeriaceae, Lenthitheciaceae, Lophiostomataceae, Massarinaceae, Montagnulaceae, Morosphaeriaceae, Phaeosphaeriaceae, Pleosporaceae, Testudinaceae and Trematosphaeriaceae. Two new families are described: Aigialaceae and Morosphaeriaceae, and three new genera proposed: Halomassarina, Morosphaeria and Rimora.
    [Show full text]
  • A Taxonomic and Phylogenetic Investigation of Conifer Endophytes
    A Taxonomic and Phylogenetic Investigation of Conifer Endophytes of Eastern Canada by Joey B. Tanney A thesis submitted to the Faculty of Graduate and Postdoctoral Affairs in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biology Carleton University Ottawa, Ontario © 2016 Abstract Research interest in endophytic fungi has increased substantially, yet is the current research paradigm capable of addressing fundamental taxonomic questions? More than half of the ca. 30,000 endophyte sequences accessioned into GenBank are unidentified to the family rank and this disparity grows every year. The problems with identifying endophytes are a lack of taxonomically informative morphological characters in vitro and a paucity of relevant DNA reference sequences. A study involving ca. 2,600 Picea endophyte cultures from the Acadian Forest Region in Eastern Canada sought to address these taxonomic issues with a combined approach involving molecular methods, classical taxonomy, and field work. It was hypothesized that foliar endophytes have complex life histories involving saprotrophic reproductive stages associated with the host foliage, alternative host substrates, or alternate hosts. Based on inferences from phylogenetic data, new field collections or herbarium specimens were sought to connect unidentifiable endophytes with identifiable material. Approximately 40 endophytes were connected with identifiable material, which resulted in the description of four novel genera and 21 novel species and substantial progress in endophyte taxonomy. Endophytes were connected with saprotrophs and exhibited reproductive stages on non-foliar tissues or different hosts. These results provide support for the foraging ascomycete hypothesis, postulating that for some fungi endophytism is a secondary life history strategy that facilitates persistence and dispersal in the absence of a primary host.
    [Show full text]
  • Phylogenetic Lineages in the Botryosphaeriaceae
    STUDIES IN MYCOLOGY 55: 235–253. 2006. Phylogenetic lineages in the Botryosphaeriaceae Pedro W. Crous1*, Bernard Slippers2, Michael J. Wingfield2, John Rheeder3, Walter F.O. Marasas3, Alan J.L. Philips4, Artur Alves5, Treena Burgess6, Paul Barber6 and Johannes Z. Groenewald1 1Centraalbureau voor Schimmelcultures, Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD, Utrecht, The Netherlands; 2Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, South Africa; 3PROMEC Unit, Medical Research Council, P.O. Box 19070, 7505 Tygerberg, South Africa; 4Centro de Recursos Microbiológicos, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal; 5Centro de Biologia Celular, Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal; 6School of Biological Sciences & Biotechnology, Murdoch University, Murdoch 6150, WA, Australia *Correspondence: Pedro W. Crous, [email protected] Abstract: Botryosphaeria is a species-rich genus with a cosmopolitan distribution, commonly associated with dieback and cankers of woody plants. As many as 18 anamorph genera have been associated with Botryosphaeria, most of which have been reduced to synonymy under Diplodia (conidia mostly ovoid, pigmented, thick-walled), or Fusicoccum (conidia mostly fusoid, hyaline, thin-walled). However, there are numerous conidial anamorphs having morphological characteristics intermediate between Diplodia and Fusicoccum, and there are several records of species outside the Botryosphaeriaceae that have anamorphs apparently typical of Botryosphaeria s.str. Recent studies have also linked Botryosphaeria to species with pigmented, septate ascospores, and Dothiorella anamorphs, or Fusicoccum anamorphs with Dichomera synanamorphs. The aim of this study was to employ DNA sequence data of the 28S rDNA to resolve apparent lineages within the Botryosphaeriaceae.
    [Show full text]
  • AR TICLE a New Family and Genus in Dothideales for Aureobasidium-Like
    IMA FUNGUS · 8(2): 299–315 (2017) doi:10.5598/imafungus.2017.08.02.05 A new family and genus in Dothideales for Aureobasidium-like species ARTICLE isolated from house dust Zoë Humphries1, Keith A. Seifert1,2, Yuuri Hirooka3, and Cobus M. Visagie1,2,4 1Biodiversity (Mycology), Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON, Canada, K1A 0C6 2Department of Biology, University of Ottawa, 30 Marie-Curie, Ottawa, ON, Canada, K1N 6N5 3Department of Clinical Plant Science, Faculty of Bioscience, Hosei University, 3-7-2 Kajino-cho, Koganei, Tokyo, Japan 4Biosystematics Division, ARC-Plant Health and Protection, P/BagX134, Queenswood 0121, Pretoria, South Africa; corresponding author e-mail: [email protected] Abstract: An international survey of house dust collected from eleven countries using a modified dilution-to-extinction Key words: method yielded 7904 isolates. Of these, six strains morphologically resembled the asexual morphs of Aureobasidium 18S and Hormonema (sexual morphs ?Sydowia), but were phylogenetically distinct. A 28S rDNA phylogeny resolved 28S strains as a distinct clade in Dothideales with families Aureobasidiaceae and Dothideaceae their closest relatives. BenA Further analyses based on the ITS rDNA region, β-tubulin, 28S rDNA, and RNA polymerase II second largest subunit black yeast confirmed the distinct status of this clade and divided strains among two consistent subclades. As a result, we Dothidiomycetes introduce a new genus and two new species as Zalaria alba and Z. obscura, and a new family to accommodate them in ITS Dothideales. Zalaria is a black yeast-like fungus, grows restrictedly and produces conidiogenous cells with holoblastic RPB2 synchronous or percurrent conidiation.
    [Show full text]
  • Proposed Generic Names for Dothideomycetes
    Naming and outline of Dothideomycetes–2014 Nalin N. Wijayawardene1, 2, Pedro W. Crous3, Paul M. Kirk4, David L. Hawksworth4, 5, 6, Dongqin Dai1, 2, Eric Boehm7, Saranyaphat Boonmee1, 2, Uwe Braun8, Putarak Chomnunti1, 2, , Melvina J. D'souza1, 2, Paul Diederich9, Asha Dissanayake1, 2, 10, Mingkhuan Doilom1, 2, Francesco Doveri11, Singang Hongsanan1, 2, E.B. Gareth Jones12, 13, Johannes Z. Groenewald3, Ruvishika Jayawardena1, 2, 10, James D. Lawrey14, Yan Mei Li15, 16, Yong Xiang Liu17, Robert Lücking18, Hugo Madrid3, Dimuthu S. Manamgoda1, 2, Jutamart Monkai1, 2, Lucia Muggia19, 20, Matthew P. Nelsen18, 21, Ka-Lai Pang22, Rungtiwa Phookamsak1, 2, Indunil Senanayake1, 2, Carol A. Shearer23, Satinee Suetrong24, Kazuaki Tanaka25, Kasun M. Thambugala1, 2, 17, Saowanee Wikee1, 2, Hai-Xia Wu15, 16, Ying Zhang26, Begoña Aguirre-Hudson5, Siti A. Alias27, André Aptroot28, Ali H. Bahkali29, Jose L. Bezerra30, Jayarama D. Bhat1, 2, 31, Ekachai Chukeatirote1, 2, Cécile Gueidan5, Kazuyuki Hirayama25, G. Sybren De Hoog3, Ji Chuan Kang32, Kerry Knudsen33, Wen Jing Li1, 2, Xinghong Li10, ZouYi Liu17, Ausana Mapook1, 2, Eric H.C. McKenzie34, Andrew N. Miller35, Peter E. Mortimer36, 37, Dhanushka Nadeeshan1, 2, Alan J.L. Phillips38, Huzefa A. Raja39, Christian Scheuer19, Felix Schumm40, Joanne E. Taylor41, Qing Tian1, 2, Saowaluck Tibpromma1, 2, Yong Wang42, Jianchu Xu3, 4, Jiye Yan10, Supalak Yacharoen1, 2, Min Zhang15, 16, Joyce Woudenberg3 and K. D. Hyde1, 2, 37, 38 1Institute of Excellence in Fungal Research and 2School of Science, Mae Fah Luang University,
    [Show full text]
  • The Genera of Fungi – G 4: Camarosporium and Dothiora
    IMA FUNGUS · 8(1): 131–152 (2017) doi:10.5598/imafungus.2017.08.01.10 ARTICLE The Genera of Fungi – G 4: Camarosporium and Dothiora Pedro W. Crous1,2,3, and Johannes Z. Groenewald1 1Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; corresponding author e-mail: p.crous@ westerdijkinstitute.nl 2Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands 3Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, P. Bag X20, Pretoria 0028, South Africa Abstract: The current paper represents the fourth contribution in the Genera of Fungi series, linking type species Key words: of fungal genera to their morphology and DNA sequence data. The present paper focuses on two genera of DNA Barcodes microfungi, Camarosporium and Dothiora, which are respectively epi- and neotypified. The genusCamarosporium fungal systematics is typified by C. quaternatum, which has a karstenula-like sexual morph, and phoma-like synasexual morph. ITS Furthermore, Camarosporomyces, Foliophoma and Hazslinszkyomyces are introduced as new camarosporium- LSU like genera, while Querciphoma is introduced as a new phoma-like genus. Libertasomycetaceae is introduced typification as a new family to accommodate Libertasomyces and Neoplatysporoides. Dothiora, which is typified by www.GeneraofFungi.org D. pyrenophora, is shown to produce dothichiza- and hormonema-like synasexual morphs in culture, and D. cactacearum is introduced as a new species. In addition to their typification, ex-type cultures have been deposited in the Westerdijk Fungal Biodiversity Institute (CBS Culture Collection), and species-specific DNA barcodes in GenBank. Authors interested in contributing accounts of individual genera to larger multi-authored papers in this series should contact the associate editors listed on the List of Protected Generic Names for Fungi.
    [Show full text]
  • Dothidea Eucalypti Fungal Planet Description Sheets 401
    400 Persoonia – Volume 39, 2017 Dothidea eucalypti Fungal Planet description sheets 401 Fungal Planet 684 – 20 December 2017 Dothidea eucalypti Crous, sp. nov. Etymology. Name refers to Eucalyptus, the host genus from which this Notes — Genera in the Dothideaceae commonly form fungus was collected. Do thi chiza and hormonema-like morphs in culture (Crous & Classification — Dothideaceae, Dothideales, Dothideomy- Groenewald 2017), which were also seen in cultures of Dothi- cetes. dea eucalypti in this study. Based on a megablast search using the ITS sequence, the Conidiomata separate, erumpent, pycnidial, brown, 50–250 µm closest matches in NCBIs GenBank nucleotide database diam with central ostiole, exuding a crystalline conidial mass; were Dothidea berberidis (GenBank EU167601; Identities wall of 3–6 layers of brown textura angularis. Conidio phores 497/515 (97 %), 3 gaps (0 %)), Dothidea ribesia (GenBank reduced to conidiogenous cells, lining the inner cavity, pale KY929142; Identities 501/515 (97 %), 3 gaps (0 %)) and Dothi- brown, smooth, doliiform, 6–10 × 4–5 µm, with central phialidic dea hippophaeos (GenBank KF147924; Identities 497/515 locus. Conidia hyaline, smooth, guttulate, aseptate, subcylin- (97 %), 2 gaps (0 %)). The highest similarities using the LSU drical, apex obtuse, base truncate, (7–)8–10(–12) × (2.5–)3 sequence were Dothidea sambuci (GenBank AF382387; Iden- µm. Hyphae 3–5 µm diam, brown, thick-walled, verruculose, tities 856/857 (99 %), no gaps), Dothidea ribesia (GenBank constricted at septa, giving rise to hormonema-like synasexual KY929175; Identities 855/857 (99 %), no gaps) and Dothidea morph. insculpta (GenBank NG_027643; Identities 854/856 (99 %), no Culture characteristics — Colonies flat, spreading, with sparse gaps).
    [Show full text]
  • Pest Risk Assessment of the Importation Into the United States of Unproc- Essed Eucalyptus Logs and Chips from South America
    United States Department of Agriculture Pest Risk Assessment Forest Service of the Importation into Forest Products Laboratory the United States of General Technical Unprocessed Eucalyptus Report FPL−GTR−124 Logs and Chips from South America A moderate pest risk potential was assigned to eleven other Abstract organisms or groups of organisms: eucalypt weevils In this report, we assess the unmitigated pest risk potential of (Gonipterus spp.), carpenterworm (Chilecomadia valdivi- importing Eucalyptus logs and chips from South America ana) on two Eucalyptus species other than E. nitens, platy- into the United States. To do this, we estimated the likeli- podid ambrosia beetle (Megaplatypus parasulcatus), yellow hood and consequences of introducing representative insects phorancantha borer (Phoracantha recurva), subterranean and pathogens of concern. Nineteen individual pest risk termites (Coptotermes spp., Heterotermes spp.), foliar assessments were prepared, eleven dealing with insects and diseases (Aulographina eucalypti, Cryptosporiopsis eight with pathogens. The selected organisms were represen- eucalypti, Cylindrocladium spp., Phaeophleospora spp., tative examples of insects and pathogens found on the foli- Mycosphaerella spp.), eucalyptus rust (Puccinia psidii), age, on the bark, in the bark, and in the wood of Eucalyptus Cryphonectria canker (Cryphonectria cubensis), Cytospora spp. Among the insects and pathogens assessed, eight were cankers (Cytospora eucalypticola, Cytospora eucalyptina), rated a high risk potential: purple moth (Sarsina
    [Show full text]
  • The Tubeufiaceae and Similar Loculoascomycetes
    Issued 10th February, 1987 Mycological Papers, No. 157 THE TUBEUFIACEAE AND SIMILAR LOCULOASCOMYCETES by AMY Y. ROSSMAN Mycology Laboratory, Plant Protection Institute United States Department of Agriculture Agricultural Research Service Beltsville Agricultural Research Center Beltsville, Maryland 20705, USA CAB INTERNATIONAL MYCOLOGICAL INSTITUTE C.A.B. International Mycological Institute (CMI), Ferry Lane, Kew, Surrey TW9 3AF, UK. Published by C.A.B International, Farnham Royal, Slough SL2 3BN, United Kingdom Tel: Farnham Common (02814) 2281 Telex: 847964 (COMAGG G) Telegrams: Comag, Slough International Dialcom: 84: CAU001 ISSN 0027-5522 ISBN 0 85198 5807 © C.A.B. International, 1987 All rights reserved. No part of this publication may be reproduced in any form or by any means, electronically, mechanically, by photocopying, recording or otherwise, without prior permission of the copyright owner. This and other publications of CAB International can be obtained through any major bookseller or direct from CAB International, Farnham Royal, Slough SL2 3BN, UK. Printed in Great Britain by the Cambrian News (Aberystwyth) Ltd SUMMARY A study of the fungi having fleshy, white to bright-coloured, uniloculate ascocarps with bitunicate asci is presented based on an examination of type specimens and all other available specimens. Fifty-three species are accepted in the Tubeufiaceae, Pleosporales. In addition, ten species similar to the Tubeufiaceae are included in this paper: one species in the Dimeriaceae, Pleosporales; four species in the Dothideaceae, Dothideales; and five species of discomycetes with bitunicate asci of uncertain disposition. Keys to sixteen genera and sixty-one species are provided. Thirty-six species are fully described and illustrated. The remaining species of Tubeufiaceae and similar Loculoascomycetes are discussed with reference to full descriptions found elsewhere.
    [Show full text]
  • Appendix 1—Reviewers and Contributers
    Appendix 1—Reviewers and Contributers The following individuals provided assistance, information, and review of this report. It could not have been completed without their cooperation. USDA APHIS-PPQ: D. Alontaga*, T. Culliney*, H. Meissner*, L. Newton* Hawai’i Department of Agriculture, Plant Industry Division: B. Kumashiro, C. Okada, N. Reimer University of Hawai’i: F. Brooks*, H. Spafford* USDA Forest Service: K. Britton*, S. Frankel* USDI Fish and Wildlife Service: D. Cravahlo Forest Research Institute Malaysia: S. Lee* 1 U.S. Department of the Interior, Geological Survey: L. Loope* Hawai’i Department of Land and Natural Resources, Division of Forestry and Wildlife: R. Hauff New Zealand Ministry for Primary Industries: S. Clark* Hawai’i Coordinating Group on Alien Pest Species: C. Martin* *Provided review comments on the draft report. 2 Appendix 2—Scientific Authorities for Chapters 1, 2, 3, and 5 Hypothenemus obscurus (F.) Kallitaxila granulatae (Stål) Insects Klambothrips myopori Mound & Morris Charaxes khasianus Butler Monema flavescens Walker Acizzia uncatoides (Ferris & Klyver) Neopithecops zalmora Butler Actias luna L. Nesopedronia dura Beardsley Adoretus sinicus (Burmeister) Nesopedronia hawaiiensis Beardsley Callosamia promethea Drury Odontata dorsalis (Thunberg) Ceresium unicolor White Plagithmysus bilineatus Sharp Chlorophorus annularis (F.) Quadrastichus erythrinae Kim Citheronia regalis Fabricus Scotorythra paludicola Butler Clastoptera xanthocephala Germ. Sophonia rufofascia Kuoh & Kuoh Cnephasia jactatana Walker Specularis
    [Show full text]