Forschungsbericht 2020 Inhaltsverzeichnis

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Forschungsbericht 2020 Inhaltsverzeichnis Stiftung Tierärztliche Hochschule Hannover Forschungsbericht 2020 Inhaltsverzeichnis Anatomisches Institut .............................................................................................................................. 2 Außenstelle für Epidemiologie (Bakum) ................................................................................................ 13 Fachgebiet Fischkrankheiten, Zentrum für Infektionsmedizin ............................................................... 19 Klinik für Geflügel .................................................................................................................................. 24 Klinik für Heimtiere, Reptilien, Zier- und Wildvögel ............................................................................... 29 Institut für Biometrie, Epidemiologie und Informationsverarbeitung ...................................................... 33 Institut für Immunologie ......................................................................................................................... 49 Klinik für kleine Klauentiere u. forensische Medizin und Ambulatorische Klinik ................................... 52 Klinik für Kleintiere ................................................................................................................................. 70 Institut für Lebensmittelqualität und -sicherheit ..................................................................................... 94 Fachgebiet Allgemeine Radiologie und Medizinische Physik ............................................................. 108 Institut für Mikrobiologie, Zentrum für Infektionsmedizin ..................................................................... 114 Institut für Parasitologie, Zentrum für Infektionsmedizin ..................................................................... 121 Institut für Pathologie ........................................................................................................................... 131 Klinik für Pferde ................................................................................................................................... 144 Institut für Pharmakologie, Toxikologie und Pharmazie ...................................................................... 157 Institut für Physiologie und Zellbiologie ............................................................................................... 163 Institut für Biochemie ........................................................................................................................... 173 Reproduktionsmedizinische Einheit der Kliniken ................................................................................ 190 Research Center for Emerging Infections and Zoonoses ................................................................... 205 Klinik für Rinder ................................................................................................................................... 224 Institut für Terrestrische und Aquatische Wildtierforschung, Büsum .................................................. 237 Institut für Terrestrische und Aquatische Wildtierforschung, Hannover .............................................. 263 Institut für Tierernährung ..................................................................................................................... 274 Institut für Tierhygiene, Tierschutz und Nutztierethologie ................................................................... 284 Institut für Tierökologie ........................................................................................................................ 301 Institut für Tierzucht und Vererbungsforschung .................................................................................. 305 Institut für Virologie, Zentrum für Infektionsmedizin ............................................................................ 317 Institut für Zoologie .............................................................................................................................. 326 ZELDA ................................................................................................................................................. 350 1 TiHo Forschungsbericht 2020 Anatomisches Institut Forschungsprofil Abteilung "Anatomie" Prof. Dr. Christiane Pfarrer Forschungsschwerpunkte: Vergleichende Plazentologie - Plazentation bei Hund und Katze - Expression von Wachstumsfaktoren in der Fleischfresserplazenta - Plazentation der Stute - Expression von Wachstumsfaktoren in der Pferdeplazenta - Hämochoriale Plazentationstypen Plazenta des Rindes - In/ex vivo Expression von Wachstumsfaktoren - Zell-Zell Interaktionen (Connexine, Integrine, Extrazelluläre Matrix, Matrixmetalloproteinasen und ihre Inhibitoren) - Bedeutung der Zell-Zell- und Zell-Matrix-Interaktionen für die Nachgeburtsverhaltung des Rindes - In-vitro-Modelle zur plazentären fetomaternalen Kommunikation, Trophoblastinvasion und Angiogenese - Expression von Transportern in Zellen der fetomaternalen Barriere und deren Beeinflussung - Plazenta von geklonten Rinderfeten - Plazentäre Veränderungen nach in vitro Kultur der Embryonen Endometrium des Rindes - Einfluss des IGF-Systems und der Galectine auf die Entwicklung postpartaler Metritiden beim Rind Funktionelle Anatomie der Gelenke der Haustiere - Morphometrie der Gelenkflächen - Struktur der Gelenkbänder Arbeitsgruppe Funktionelle Anatomie Prof. Dr. Hagen Gasse Forschungsschwerpunkte: #Anatomie/Histologie des Stimmapparats - Histologie/Histomorphometrie und Korrosionsanatomische Untersuchung der Plica vocalis #Craniometrie und Encephalometrie - Klinisch/neurologische und archäozoologische Schwerpunkte #Bildgebende Verfahren Abteilung "Funktionelle Histologie und Zellbiologie" Prof. Dr. Ralph Brehm Forschungsschwerpunkte: Der Forschungsschwerpunkt der Abteilung bzw. Arbeitsgruppe Brehm liegt auf dem Gebiet der männlichen Reproduktionsmedizin. Aktuelle Forschungsprojekte beschäftigen sich vor allem mit der direkten Zell-Zell-Kommunikation über Connexine im Hoden verschiedener Spezies, der Rolle dieser Gap Junction Proteine in der normalen Spermatogenese und mit der Relevanz von Connexin43 in der Pathogenese caniner und humaner Hodentumore. Ein weiterer Schwerpunkt der Forschung liegt auf Untersuchungen zur Bildung, Zusammensetzung und Regulation der Blut-Hoden-Schranke (mit Claudin-3, -5, -11 und Occludin). Genutzt werden sowohl funktionelle, transgene Tiermodelle als auch ein breites, zell- und molekularbiologisches Methodenspektrum. Dienstleistungsangebot: Einbettautomat und Paraffinausgießstation, Histologische Auswertungen von z.B. Hodenbiopsien zur Fertilitätsdiagnostik TiHo Forschungsbericht 2020 2 Forschungsprojekte 1. Auswirkungen einer Keimzell-spezifischen Deletion des Connexin43-Gens auf die Regulation der Spermatogenese. Effects of a germ cell-specific deletion of connexin43 on the regulation of spermatogenesis. Prof. Dr. Ralph Brehm Dr. Kristina Rode In diesem transgenen Tiermodell werden die Folgen einer Deletion von Connexin43 in Keimzellen auf die Entwicklung des Hodens und die Spermatogenese histologisch und immunhistochemisch untersucht. Verwendet werden hier Keimzell-spezifische VASA-Cre und Cx43 gefloxte Mauslinien. Laufzeit: April 2009 bis Dezember 2021 Kooperationspartner: PD Dr. Daniela Fietz, JLU Giessen Prof. Dr. Martin Bergmann, JLU Giessen 2. Auswirkungen einer Sertoli Zell-spezifischen Deletion des Connexin43-Gens auf die Regulation der Spermatogenese in transgenen Mäusen unter Verwendung des Cre/loxP- Rekombinasesystems Effects of a Sertoli cell (SC) specific knockout of the connexin43-gene on the regulation of spermatogenesis in transgenic mice using the Cre/loxP-recombination system Prof. Dr. Ralph Brehm Dr. Kristina Rode Erika Hilbold In diesem Projekt werden in einem transgenen Mausmodell die Folgen einer Sertoli Zell- spezifischen Deletion des Connexin43-Gens auf die Regulation der Spermatogenese u.a. mittels Microarray und qRT-PCR untersucht. Ausgewählte signifikant veränderte Kandidatengene aus den Mausversuchen werden zudem mit Hodenbiopsien von infertilen Männern verglichen. Das Projekt bildet die Grundlage verschiedener Forschungsprojekte und Dissertationen sowie künftiger Drittmittelprojekte. Resultate: https://www.sciencedirect.com/science/article/pii/S1642431X17302371?via%3Dihub Laufzeit: Mai 2008 bis Dezember 2021 Kooperationspartner: Dr. Daniela Fietz, JLU Giessen Klinische Forschergruppe Giessen + Marburg http://www.uni-giessen.de/cms/fbz/fb11/forschung/forschergruppen/kfo_181/home 3. Auswirkungen einer Sertoli Zell-spezifischen Deletion des Connexin45-Gens auf die Regulation der Spermatogenese in transgenen Mäusen unter Verwendung des Cre/loxP- Rekombinasesystems Effects of a Sertoli cell (SC) specific knockout of the connexin45-gene on the regulation of spermatogenesis in transgenic mice using the Cre/loxP-recombination system Prof. Dr. Ralph Brehm Dr. Kristina Rode Erika Hilbold In diesem Projekt werden in einem transgenen Mausmodell die Folgen einer Sertoli Zell- spezifischen Deletion des Connexin45-Gens auf die Regulation der Spermatogenese u.a. mittels 3 TiHo Forschungsbericht 2020 IHC und WB untersucht. Die zelluläre Lokalisation von Connexin45 im Hoden adulter Mäuse soll mittels Cx45EGFP-Mäusen bestätigt werden. Laufzeit: Mitte 2012 bis Dezember 2021 Kooperationspartner: Frau PD Dr. Karin Dedek, Universität Oldenburg. 4. Auswirkungen eines Keimzell-spezifischen Knockouts des Connexin 43-Gens auf die Spermatogenese in transgenen
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